Compound spray disinfectant for farms and preparation process of compound spray disinfectant
By preparing a compound spray disinfectant consisting of a lignin-based electrostatic generator and a carboxymethyl chitosan derivative, and utilizing the combination of electrostatic adsorption and traditional Chinese medicine extracts, the problems of low sterilization efficiency and high ammonia content in livestock and poultry farms were solved, achieving efficient and low-toxicity sterilization and ammonia removal effects.
Patent Information
- Application Number
- CN202510964375.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-14
- Publication Date
- 2025-10-17
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Existing chemical disinfectants have problems such as high corrosivity, strong irritation, high drug resistance, and inability to effectively reduce ammonia content in livestock and poultry farms. Traditional Chinese medicine preparations have low bactericidal efficiency in spray disinfectants and cannot quickly bind with airborne harmful bacteria.
A compound spray disinfectant was prepared by combining a lignin-based electrostatic generator and a carboxymethyl chitosan derivative through ultrasonic treatment. The electrostatic adsorption generated by the electrostatic generator rapidly kills bacteria, and the compounding with traditional Chinese medicine extracts achieves efficient sterilization and ammonia removal.
It achieves highly efficient sterilization and ammonia removal effects with low toxicity and no residue, improves sterilization efficiency and storage stability, and solves the problems of low sterilization efficiency of traditional Chinese medicine spray disinfectants and drug resistance of chemical disinfectants.
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Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the technical field of biological disinfection, and particularly relates to a compound spray disinfectant for farms and a preparation process thereof. BACKGROUND
[0002] The livestock and poultry farms often contain a large amount of planktonic harmful bacteria and harmful gases such as ammonia generated by fecal fermentation, and both the planktonic harmful bacteria and the ammonia will cause harm to the health of animals if their content is too high. Improving the ventilation condition in the livestock and poultry farms and timely spraying disinfectants into the livestock and poultry farms are the main measures to control the planktonic harmful bacteria and harmful gases in the livestock and poultry farms.
[0003] At present, the commonly used disinfectants in the livestock and poultry farms are mostly chemical disinfectants, such as peroxoacetic acid, glutaraldehyde cetalkonium bromide, benzalkonium chloride, etc. Most of them have the characteristics of strong corrosion, strong irritation and high drug resistance frequency. After disinfecting the farms, the ground and feeding troughs need to be cleaned and ventilated for a long time to avoid the residues of the disinfectants, and most of them cannot effectively reduce the content of ammonia. Therefore, it is necessary to develop a high-efficiency, low-toxicity and residue-free chemical disinfectant substitute.
[0004] Traditional Chinese medicine preparations have the characteristics of pure natural and low toxicity, and have good killing effect on pathogenic microorganisms. However, compared with chemical disinfectants, most of them cannot achieve efficient and rapid sterilization in the solution system through high ionization rate and strong penetration of charged particles like chemical disinfectants. The traditional Chinese medicine preparations applied in the spray disinfectant cannot quickly combine with the planktonic harmful bacteria in the livestock and poultry farms, and have the problem of low sterilization efficiency. SUMMARY
[0005] In order to solve the above technical defects, the application researches a compound spray disinfectant for farms and a preparation process thereof. The prepared compound spray disinfectant can efficiently kill bacteria and remove ammonia, and has the characteristics of low toxicity and environmental protection.
[0006] A compound spray disinfectant for farms and a preparation process thereof are prepared from the following raw materials by weight: 100-120 parts of a traditional Chinese medicine bactericidal composition, 25-30 parts of a lignin-based static electricity generator, 10-15 parts of a bactericidal synergistic stabilizer, 200-250 parts of methyl oleate and 400-500 parts of distilled water. The preparation process comprises the following steps: The bactericidal synergistic stabilizer and the traditional Chinese medicine bactericidal composition are added into distilled water and stirred to disperse uniformly, then the lignin-based static generator is added, and the mixture is placed in a cell crusher for ultrasonic treatment at an ultrasonic frequency of 25-30 kHz for 20-25 minutes to obtain a water dispersant, then methyl oleate is added dropwise into the water dispersant, and the mixture is transferred to the cell crusher for treatment at an ultrasonic frequency of 35-40 kHz for 12-15 minutes to obtain the compound spray disinfectant.
[0007] Further, the preparation method of the lignin-based static generator comprises the following steps: S1.1: hyaluronic acid, sodium lignosulfonate and phytic acid are mixed in deionized water at a mass ratio of 1:(0.5-1):(2-5), and the mass of deionized water is 80-100 times that of hyaluronic acid, to obtain solution A; acrylamide, N,N'-methylenebisacrylamide and ammonium persulfate are dissolved in deionized water at a mass ratio of 1:(0.008-0.01):(0.003-0.005), and the mass of deionized water is 40-45 times that of acrylamide, to obtain solution B; S1.2: solution A and solution B are mixed at a mass ratio of 1:(1-1.2), then 0.25-0.3wt% of tetramethyl ethylenediamine and 1.5-2wt% of zinc oxide are added, the mixture is transferred to a reaction kettle for sealing, and then reacted at 55-60℃ for 4-5 hours to obtain a lignin-based hydrogel, the lignin-based hydrogel is pre-frozen at-20℃ for 20-24 hours, then transferred to a freeze dryer for freeze-drying at-50 to-45℃ for 40-45 hours, and then ground to obtain a lignin-based aerogel powder; S1.3: 3-4 parts of the lignin-based aerogel powder are placed in a large container, 1 part of 1H, 1H, 2H, 2H-perfluorooctyltrichlorosilane is placed in a small container, the small container is sealed, and then the large container is placed in an oven at 90-95℃ for 4-5 hours, then the sample is taken out and heated in an oven at 100-105℃ for 25-30 minutes to obtain a lignin-based static generator.
[0008] Further, the particle size of the lignin-based aerogel powder in step S1.2 is 60-80μm.
[0009] Further, the volume ratio of the small container to the large container in step S1.3 is 1:(25-30).
[0010] Further, the preparation method of the traditional Chinese medicine bactericidal composition comprises the following steps: S2.1: add 100-120 parts of distilled water in a container, heat to 40-45℃, then add 0.5-0.7 parts of evodia extract, 0.4-0.6 parts of acacia extract, 0.3-0.5 parts of andrographis extract and 0.2-0.4 parts of melia azedarach bark extract in turn, stir until the solution is clear and free of particles, cool to room temperature naturally, obtain the bacteriostatic composite solution; S2.2: add 0.4-0.6 parts of soapberry extract and 0.6-0.8 parts of khus extract to the bacteriostatic composite solution prepared in step S2.1, stir at 400-500 rpm for 10-15 minutes, prepare the traditional Chinese medicine bactericidal composition.
[0011] Further, the preparation method of the bactericidal synergistic stabilizer comprises the following steps: S3.1: dissolve carboxymethyl chitosan in distilled water to prepare a carboxymethyl chitosan solution with a mass fraction of 2.5-3%, then add a hydrochloric acid solution with a concentration of 1-1.2 mol / L to adjust the pH to 3.4-3.6, obtain a pre-reaction solution; S3.2: add 2.5-3% of formaldehyde, 8.5-9% of octylamine and 10-12% of cyclohexyl isonitrile based on the mass of carboxymethyl chitosan in the pre-reaction solution, stir at room temperature and 2000-3000 rpm for 30-40 minutes, then adjust the stirring speed to 250-300 rpm and continue stirring for 24-30 hours, obtain a reaction solution; S3.3: place the reaction solution in a dialysis bag with a molecular weight cut-off of 3000-3500 for deionized water dialysis, then transfer to a freeze dryer for freeze drying, obtain the bactericidal synergistic stabilizer.
[0012] Further, the carboxymethyl chitosan in step S3.1 has a carboxylation degree of 80-90%.
[0013] Further, the deionized water dialysis time in step S3.2 is 5-6 days, and the deionized water is replaced every 10-12 hours during dialysis.
[0014] The beneficial effects are: 1, the lignin-based hydrogel is prepared, then freeze-drying is carried out, lignin-based aerogel powder with porosity is prepared, then the surface is modified by chemical vapor deposition with 1H, 1H, 2H, 2H-perfluorooctyltrichlorosilane, and lignin-based static generator with high hydrophobic surface is prepared, the lignin-based static generator is introduced into the compound spray disinfectant, when the lignin-based static generator is sprayed at high speed, the small particle size droplets collide and rub with the lignin-based static generator with high hydrophobicity, the contact charging effect is generated, under the zinc oxide strengthening effect, the droplets containing bactericidal active ingredients are positively charged, when the positively charged droplets are dispersed in the space of the farm, the electrostatic adsorption effect with the negative charge of the bacterial cell wall or cell membrane is generated, so that the outer structure of the bacteria is quickly destroyed, the bactericidal intensity and bactericidal efficiency of the active ingredients are effectively increased, and the problem of low bactericidal efficiency of the traditional compound disinfectant is solved.
[0015] 2, the carboxymethyl chitosan is prepared into a solution, then formaldehyde, octylamine and cyclohexyl isonitrile are added to react, the carboxymethyl chitosan derivative with amphiphilic property is prepared, then the bactericidal synergistic stabilizer is prepared by dialysis, so that the lignin-based static generator is stably dispersed in the compound spray disinfectant, the effective collision and rubbing of the droplets and the lignin-based static generator during spraying are improved, the contact charging effect is strengthened, the storage stability of the compound spray disinfectant is improved, and the bactericidal efficiency of the active ingredients is also improved.
[0016] 3, the traditional Chinese medicine bactericidal composition is prepared by compounding evodia extract, myrobalan extract, andrographis extract, melia azedarach bark extract, Chinese waxgourd extract and shiitake mushroom extract, wherein the evodia extract, myrobalan extract, andrographis extract and melia azedarach bark extract can inhibit the enzyme synthesis of bacteria and fungi, penetrate the cell wall of bacteria, and interfere with the RNA synthesis of parasitic viruses, and a large amount of organic acid in the shiitake mushroom extract can effectively neutralize ammonia gas in the farm, and generate odorless and non-toxic organic salt, and the saponin component in the Chinese waxgourd extract has excellent surface activity and decontamination ability, which can effectively remove the generated organic salt, so as to avoid affecting the bactericidal effect of other traditional Chinese medicine components, so that the compound spray disinfectant prepared in this way is low in toxicity and residue, has good bactericidal capacity and ammonia removal capacity. DETAILED DESCRIPTION
[0017] The technical solutions in the embodiments of the present application will be described below, obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the present application.
[0018] It should be noted that the following examples have no special description. The parts represent weight parts.
[0019] Embodiment 1 The application discloses a compound spray disinfectant for a farm and a preparation process thereof, which is prepared from the following raw materials in parts by weight: 100 parts of a traditional Chinese medicine bactericidal composition, 25 parts of a lignin-based static electricity generator, 10 parts of a bactericidal synergistic stabilizer, 200 parts of methyl oleate and 400 parts of distilled water. The preparation process comprises the following steps: The bactericidal synergistic stabilizer and the traditional Chinese medicine bactericidal composition are added into the distilled water and stirred to be uniformly dispersed, then the lignin-based static electricity generator is added, and the mixture is placed in a cell crusher and subjected to ultrasonic treatment at an ultrasonic frequency of 25 kHz for 20 minutes to obtain a water dispersant; the methyl oleate is added dropwise into the water dispersant, and then the mixture is transferred into the cell crusher and subjected to ultrasonic treatment at an ultrasonic frequency of 35 kHz for 12 minutes to obtain the compound spray disinfectant.
[0020] The preparation method of the lignin-based static electricity generator comprises the following steps: S1.1: hyaluronic acid, sodium lignosulfonate and phytic acid are mixed in a mass ratio of 1:0.5:2 and dissolved in deionized water, and the mass of the deionized water is 80 times that of the mass of the hyaluronic acid, to obtain a solution A; acrylamide, N,N'-methylenebisacrylamide and ammonium persulfate are dissolved in deionized water in a mass ratio of 1:0.008:0.003, and the mass of the deionized water is 40 times that of the mass of the acrylamide, to obtain a solution B; S1.2: the solution A and the solution B are mixed in a mass ratio of 1:1, then 0.25wt% of tetramethyl ethylenediamine and 1.5wt% of zinc oxide are added, the mixture is transferred into a reaction kettle and sealed, then the mixture is reacted at 55°C for 4 hours to obtain a lignin-based hydrogel, the lignin-based hydrogel is pre-frozen at-20°C for 20 hours, then the lignin-based hydrogel is transferred into a freeze dryer and freeze-dried at-50°C for 40 hours, and then the lignin-based aerogel powder with a particle size of 60μm is obtained after crushing and grinding; S1.3: 3 parts of the lignin-based aerogel powder are placed in a large container, 1 part of 1H, 1H, 2H, 2H-perfluorooctyltrichlorosilane is filled in a small container which is placed in the large container, and the volume ratio of the small container to the large container is 1:25, then the large container is sealed and placed in an oven at 90°C for 4 hours, and then the sample is taken out and heated in an oven at 100°C for 25 minutes to obtain the lignin-based static electricity generator.
[0021] The preparation method of the traditional Chinese medicine bactericidal composition comprises the following steps: S2.1: 100 parts of distilled water are added into a container, heated to 40°C, and then 0.5 parts of evodia extract, 0.4 parts of chebula extract, 0.3 parts of andrographis extract and 0.2 parts of bitter ash bark extract are sequentially added, stirred until the solution is clear and free of particles, and then naturally cooled to room temperature to obtain an antibacterial composite solution. S2.2: 0.4 parts of soapberry extract and 0.6 parts of trichoderma extract were added into the bacteriostatic composite solution prepared in step S2.1, and stirred at 400 rpm for 10 minutes to prepare a traditional Chinese medicine bactericidal composition.
[0022] The preparation method of the bactericidal synergistic stabilizer comprises the following steps: S3.1: carboxymethyl chitosan with a carboxylation degree of 80% was dissolved in distilled water to prepare a carboxymethyl chitosan solution with a mass fraction of 2.5%, and then a hydrochloric acid solution with a concentration of 1 mol / L was added to adjust the pH to 3.4 to obtain a pre-reaction solution; S3.2: 2.5% of formaldehyde, 8.5% of octylamine and 10% of cyclohexyl isonitrile based on the mass of carboxymethyl chitosan were sequentially added into the pre-reaction solution, and stirred at room temperature and 2000 rpm for 30 minutes, and then the stirring speed was adjusted to 250 rpm and continued to stir for 24 hours to obtain a reaction solution; S3.3: the reaction solution was placed in a dialysis bag with a molecular weight cut-off of 3000 for deionized water dialysis, and the deionized water dialysis time was 5 days, and the deionized water was replaced every 10 hours during dialysis, and then transferred to a freeze dryer for freeze drying to obtain the bactericidal synergistic stabilizer.
[0023] Example 2 A compound spray disinfectant for farms and its preparation process, which is composed of the following raw materials by weight: 120 parts of traditional Chinese medicine bactericidal composition, 30 parts of lignin-based static electricity generator, 15 parts of bactericidal synergistic stabilizer, 250 parts of methyl oleate and 500 parts of distilled water; The preparation process comprises the following steps: The bactericidal synergistic stabilizer and the traditional Chinese medicine bactericidal composition were added into the distilled water and stirred to disperse uniformly, then the lignin-based static electricity generator was added, and placed in a cell crusher for ultrasonic treatment at an ultrasonic frequency of 25 kHz for 20 minutes to obtain a water dispersant, then the methyl oleate was added dropwise into the water dispersant, and then transferred to the cell crusher for treatment at an ultrasonic frequency of 35 kHz for 12 minutes to obtain the compound spray disinfectant.
[0024] The preparation method of the lignin-based static electricity generator comprises the following steps: S1.1: hyaluronic acid, sodium lignosulfonate and phytic acid were mixed in a mass ratio of 1:1:5 and dissolved in deionized water, and the mass of the deionized water was 100 times that of the mass of the hyaluronic acid, to obtain solution A; acrylamide, N,N'-methylene bisacrylamide and ammonium persulfate were dissolved in deionized water in a mass ratio of 1:0.01:0.005, and the mass of the deionized water was 45 times that of the mass of the acrylamide, to obtain solution B; S1.2: Solution A is mixed with solution B at a mass ratio of 1:1.2, then 0.3wt% of tetramethyl ethylenediamine and 2wt% of zinc oxide are added, transferred to a reaction kettle for sealing, then reacted at 55℃ for 4 hours to obtain a lignin-based hydrogel, the lignin-based hydrogel is pre-frozen at-20℃ for 20 hours, then transferred to a freeze dryer for freeze drying at-50℃ for 40 hours, then ground and crushed to obtain a lignin-based aerogel powder with a particle size of 60μm; S1.3: 4 parts of lignin-based aerogel powder are placed in a large container, 1 small container is placed in the large container, the small container is filled with 2 parts of 1H, 1H, 2H, 2H-perfluorooctyltrichlorosilane, the volume ratio of the small container to the large container is 1:30, then the large container is sealed and placed in an oven at 90℃ for 4 hours, then the sample is taken out and placed in an oven at 100℃ for 25 minutes to obtain a lignin-based electrostatic generator.
[0025] The preparation method of the traditional Chinese medicine bactericidal composition comprises the following steps: S2.1: 120 parts of distilled water are added to a container, heated to 40℃, then 0.7 parts of evodia extract, 0.6 parts of chebula extract, 0.5 parts of andrographis extract and 0.4 parts of melia azedarach bark extract are added in sequence, stirred until the solution is clear and free of particles, and then naturally cooled to room temperature to obtain a bacteriostatic composite solution; S2.2: 0.6 parts of soapberry extract and 0.8 parts of ganoderma lucidum extract are added to the bacteriostatic composite solution prepared in step S2.1, stirred at 400rpm for 10 minutes to prepare a traditional Chinese medicine bactericidal composition.
[0026] The preparation method of the bactericidal synergistic stabilizer comprises the following steps: S3.1: carboxymethyl chitosan with a carboxylation degree of 80% is dissolved in distilled water to prepare a carboxymethyl chitosan solution with a mass fraction of 3%, then a hydrochloric acid solution with a concentration of 1mol / L is added to adjust the pH to 3.4 to obtain a pre-reaction solution; S3.2: 3% of formaldehyde, 9% of octylamine and 12% of cyclohexyl isonitrile based on the mass of carboxymethyl chitosan are added in sequence to the pre-reaction solution, stirred at room temperature and 2000rpm for 30 minutes, then the stirring speed is adjusted to 250rpm and stirring is continued for 24 hours to obtain a reaction solution; S3.3: the reaction solution is placed in a dialysis bag with a molecular weight cut-off of 3000 for deionized water dialysis, the deionized water dialysis time is 5d, and the deionized water is replaced every 10 hours during dialysis, then transferred to a freeze dryer for freeze drying to obtain a bactericidal synergistic stabilizer.
[0027] Example 3 A farm compound spray disinfectant and a preparation process thereof, which are composed of the following raw materials in parts by weight: 100 parts of a traditional Chinese medicine bactericidal composition, 25 parts of a lignin-based static electricity generator, 10 parts of a bactericidal synergistic stabilizer, 200 parts of methyl oleate and 400 parts of distilled water; The preparation process comprises the following steps: The bactericidal synergistic stabilizer and the traditional Chinese medicine bactericidal composition are added into the distilled water and stirred to disperse uniformly, then the lignin-based static electricity generator is added, and the mixture is placed in a cell crusher for ultrasonic treatment at an ultrasonic frequency of 30 kHz for 25 minutes to obtain a water dispersant, the methyl oleate is added dropwise into the water dispersant, and then the mixture is transferred into the cell crusher for treatment at an ultrasonic frequency of 40 kHz for 15 minutes to obtain the compound spray disinfectant.
[0028] The preparation method of the lignin-based static electricity generator comprises the following steps: S1.1: hyaluronic acid, sodium lignosulfonate and phytic acid are mixed in a mass ratio of 1:0.5:2 and dissolved in deionized water, the mass of the deionized water is 80 times that of the mass of the hyaluronic acid, to obtain solution A; acrylamide, N,N'-methylenebisacrylamide and ammonium persulfate are dissolved in deionized water in a mass ratio of 1:0.008:0.003, the mass of the deionized water is 40 times that of the mass of the acrylamide, to obtain solution B; S1.2: solution A and solution B are mixed in a mass ratio of 1:1, then 0.25wt% of tetramethyl ethylenediamine and 1.5wt% of zinc oxide are added, the mixture is transferred into a reaction kettle for sealing, and then the mixture is reacted at 60℃ for 5 hours to obtain a lignin-based hydrogel, the lignin-based hydrogel is pre-frozen at-20℃ for 24 hours, then transferred into a freeze dryer for freeze drying at-45℃ for 45 hours, and then pulverized and ground to obtain lignin-based aerogel powder with a particle size of 80μm; S1.3: 3 parts of the lignin-based aerogel powder are placed in a large container, 1 part of 1H,1H,2H,2H-perfluorooctyltrichlorosilane is filled in a small container placed in the large container, the volume ratio of the small container to the large container is 1:25, then the large container is sealed and placed in an oven at 95℃ for 5 hours, and then the sample is taken out and heated in an oven at 105℃ for 30 minutes to obtain the lignin-based static electricity generator.
[0029] The preparation method of the traditional Chinese medicine bactericidal composition comprises the following steps: S2.1: 100 parts of distilled water are added into a container, heated to 45℃, then 0.5 parts of evodia extract, 0.4 parts of chebula extract, 0.3 parts of andrographis extract and 0.2 parts of melia azedarach bark extract are sequentially added, stirred until the solution is clear and free of particles, and then naturally cooled to room temperature to obtain an antibacterial composite solution; S2.2: 0.4 parts of Spondias extract and 0.6 parts of Trametes versicolor extract were added into the bacteriostatic composite solution prepared in step S2.1, and stirred at 500 rpm for 15 minutes to prepare a traditional Chinese medicine bactericidal composition.
[0030] The preparation method of the bactericidal synergistic stabilizer comprises the following steps: S3.1: carboxymethyl chitosan with a degree of carboxylation of 90% was dissolved in distilled water to prepare a carboxymethyl chitosan solution with a mass fraction of 2.5%, and then a hydrochloric acid solution with a concentration of 1.2 mol / L was added to adjust the pH to 3.6 to obtain a pre-reaction solution; S3.2: formaldehyde accounting for 2.5% of the mass of carboxymethyl chitosan, octylamine accounting for 8.5%, and cyclohexyl isonitrile accounting for 10% were sequentially added into the pre-reaction solution, and stirred at room temperature and 3000 rpm for 40 minutes, and then the stirring speed was adjusted to 300 rpm and continued to stir for 30 hours to obtain a reaction solution; S3.3: the reaction solution was placed in a dialysis bag with a molecular weight cut-off of 3500 for deionized water dialysis, and the deionized water dialysis time was 6 days, and the deionized water was replaced every 12 hours during dialysis, and then transferred to a freeze dryer for freeze drying to obtain the bactericidal synergistic stabilizer.
[0031] Comparative Example 1: The difference between Comparative Example 1 and Example 1 is that the compound spray disinfectant prepared in Comparative Example 1 does not contain lignin-based static electricity generator.
[0032] Comparative Example 2: The difference between Comparative Example 2 and Example 1 is that the compound spray disinfectant prepared in Comparative Example 2 does not contain bactericidal synergistic stabilizer.
[0033] Comparative Example 3: The difference between Comparative Example 3 and Example 1 is that the traditional Chinese medicine bactericidal composition in the compound spray disinfectant prepared in Comparative Example 3 is replaced by peracetic acid disinfectant with the same mass and effective ingredient content.
[0034] Experiment 1: The compound spray disinfectants prepared in Examples 1-3 and Comparative Examples 1-3 were taken as samples, and air disinfection test of the samples was carried out in the same experimental conditions according to the air disinfection test operation procedure in the "Disinfection Technical Specification", the temperature of the aerosol cabinet was 25°C, the relative humidity was 60%, the pressure of the 0.5 cm diameter spray gun was set to 0.5 kPa, the spray flow was 5 mL / min, 1.5 mL of ammonia water with a concentration of 25% was first sprayed into the aerosol cabinet to make the ammonia mass concentration in the aerosol cabinet 50 mg / m 3 After the ammonia mass concentration display value of the sensor was stable, the ammonia mass concentration was recorded as m0, and 5 mL of 5×10 6cfu / mL of the active bacterial suspension was sprayed into the aerosol cabinet, stirred for 5 minutes, and then allowed to stand for 5 minutes. The liquid impact sampler was used to sample the air, and the bacterial count V0 was calculated. Then 15 mL / m 3 The sample was sprayed in an aerosol cabinet and allowed to stand for 1 hour. Samples were taken at 30 minutes and 1 hour to calculate the bacterial content in the air V1. The ammonia concentration was recorded as m1. The sterilization rate was calculated as (V0-V1) / V0×100%, and the ammonia elimination rate was calculated as (m0-m1) / m0×100%. Escherichia coli, Staphylococcus aureus, and Salmonella were used as the active bacteria, and each different sample was tested three times. The test results were averaged. The sterilization rate results are shown in Table 1, and the ammonia elimination rate results are shown in Table 2.
[0035] Table 1: Killing rate of bacteria by samples at different time points
[0036] Table 2: Ammonia elimination rate of samples at different time points
[0037] From the data of Examples 1-3 in Table 1 and Table 2, it can be seen that the compound spray disinfectants prepared in Examples 1-3 all have high efficiency and strong bactericidal ability, and can effectively remove ammonia in the air; From the data of Comparative Example 1 in Table 1, it can be seen that the lignin-based electrostatic generator was not added to the compound spray disinfectant. The sterilization rate of 30 min was greatly reduced compared with that in Example 1, indicating that the sterilization efficiency was greatly reduced, and the 1 h sterilization rate also decreased, indicating that the sterilization intensity was also reduced. It can be proved that the introduction of the lignin-based electrostatic generator into the compound spray disinfectant can effectively increase the sterilization intensity and sterilization efficiency. Similarly, the data of Comparative Example 2 can prove that the sterilization synergistic stabilizer also has the effect of enhancing the sterilization efficiency and sterilization intensity of the compound spray disinfectant. It can be seen from the data of Comparative Example 3 in Tables 1 and 2 that although traditional chemical disinfectants have good bactericidal ability and can remove ammonia to a certain extent, their ammonia removal ability is far inferior to that of the compound spray disinfectants prepared in Examples 1-3. This proves that the traditional Chinese medicine bactericidal composition of the present application can provide excellent ammonia removal ability while providing excellent bactericidal ability.
[0038] Experiment 2: Three portions of the compound spray disinfectant prepared in Examples 1-3 and Comparative Example 2 were taken and stored at room temperature for 30 days to observe whether stratification, precipitation, adhesion, or agglomeration occurred. If no such phenomenon occurred, it was recorded as stable; otherwise, it was recorded as unstable. The results are shown in Table 3.
[0039] Table 3: Stability of compound spray disinfectant stored at room temperature for 30 days
[0040] As can be seen from the results of Table 3, Examples 1-3 have high storage stability, while Comparative Example 2 is unstable, which proves that the bactericidal synergistic stabilizer prepared in the present application can make the lignin-based static agent stably disperse in the compound spray disinfectant, and improve the storage stability of the compound spray disinfectant.
[0041] The above examples are only illustrative of the principles and effects of the present application, and are not intended to limit the present application. Any person skilled in the art can modify or change the above examples without departing from the spirit and scope of the present application. Therefore, all equivalent modifications or changes made by those skilled in the art without departing from the spirit and technical ideas disclosed in the present application should be covered by the claims of the present application.
Claims
1. A compound spray disinfectant for farms, characterized in that: The method comprises the following raw materials in parts by weight: 100-120 parts of a traditional Chinese medicine bactericidal composition, 25-30 parts of a lignin-based static electricity generator, 10-15 parts of a bactericidal synergistic stabilizer, 200-250 parts of methyl oleate and 400-500 parts of distilled water; The preparation method of the lignin-based static electricity generator comprises the following steps: dissolving hyaluronic acid, sodium lignin sulfonate and phytic acid in water to obtain solution A; dissolving acrylamide, N,N′-methylenebisacrylamide and ammonium persulfate in water to obtain solution B; mixing solution A and solution B, adding 0.3 wt% of tetramethylethylenediamine and 1.5-2 wt% of zinc oxide, heating the mixture to obtain a lignin-based hydrogel, freeze-drying and crushing the mixture, and then performing surface superhydrophobic modification to obtain the lignin-based static electricity generator.
2. A compound spray disinfectant for farms according to claim 1, characterized in that: The preparation method of the lignin-based electrostatic generator specifically comprises the following steps: S1.1: Hyaluronic acid, sodium lignin sulfonate, and phytic acid are mixed and dissolved in deionized water at a mass ratio of 1:(0.5-1):(2-5), where the mass of deionized water is 80-100 times the mass of hyaluronic acid, to obtain solution A. Acrylamide, N,N′-methylenebisacrylamide, and ammonium persulfate are dissolved in deionized water at a mass ratio of 1:(0.008-0.01):(0.003-0.005), where the mass of deionized water is 40-45 times the mass of acrylamide, to obtain solution B. S1.2: Solution A and solution B are mixed in a mass ratio of 1:(1-1.2), and then 0.25-0.3 wt% of tetramethylethylenediamine and 1.5-2 wt% of zinc oxide are added. The mixture is transferred to a sealed reactor and then reacted at 55-60°C for 4-5 hours to obtain a lignin-based hydrogel. The lignin-based hydrogel is pre-frozen at -20°C for 20-24 hours, and then transferred to a freeze dryer and freeze-dried at -50 to -45°C for 40-45 hours, and then pulverized and ground to obtain a lignin-based aerogel powder. S1.3: Take 3-4 parts of lignin-based aerogel powder and place it in a large container. Place a small container in the large container, and fill the small container with 1-2 parts of 1H,1H,2H,2H-perfluorooctyltrichlorosilane. Then seal the large container and place it in an oven at 90-95°C for 4-5 hours. Then take out the sample and place it in an oven at 100-105°C and heat it for 25-30 minutes to obtain a lignin-based static electricity generator.
3. The compound spray disinfectant for farms according to claim 2, characterized in that: In the method for preparing the lignin-based electrostatic generator, the particle size of the lignin-based aerogel powder in step S1.2 is 60-80 μm.
4. A compound spray disinfectant for farms according to claim 2, characterized in that: In the preparation method of the lignin-based electrostatic generator, the volume ratio of the small container to the large container in step S1.3 is 1:(25-30).
5. The compound spray disinfectant for farms according to claim 1, characterized in that: The preparation method of the Chinese medicinal bactericidal composition specifically comprises the following steps: S2.1: Add 100-120 parts of distilled water to a container, raise the temperature to 40-45°C, then add 0.5-0.7 parts of Evodia rutaecarpa extract, 0.4-0.6 parts of Terminalia chebula extract, 0.3-0.5 parts of Andrographis paniculata extract, and 0.2-0.4 parts of Melia azedarach bark extract in sequence, stir until the solution is clear and free of particles, and naturally cool to room temperature to obtain an antibacterial composite solution; S2.2: Add 0.4-0.6 parts of soapberry extract and 0.6-0.8 parts of shilajit extract to the antibacterial composite solution prepared in step S2.1, and stir at 400-500 rpm for 10-15 minutes to prepare a traditional Chinese medicine bactericidal composition.
6. The compound spray disinfectant for farms according to claim 1, characterized in that: The preparation method of the bactericidal synergistic stabilizer specifically comprises the following steps: S3.1: Dissolve carboxymethyl chitosan in distilled water to prepare a 2.5-3% carboxymethyl chitosan solution, then add 1-1.2 mol / L hydrochloric acid solution to adjust the pH to 3.4-3.6 to obtain a pre-reaction solution; S3.2: Add 2.5-3% formaldehyde, 8.5-9% octylamine, and 10-12% cyclohexyl isocyanide, based on the weight of the carboxymethyl chitosan, to the pre-reaction solution in sequence, and stir at room temperature and 2000-3000 rpm for 30-40 minutes. Then adjust the stirring speed to 250-300 rpm and continue stirring for 24-30 hours to obtain a reaction solution. S3.3: The reaction solution is placed in a dialysis bag with a molecular weight cut-off of 3000-3500 for dialysis against deionized water, and then transferred to a freeze dryer for freeze drying to obtain a bactericidal synergistic stabilizer.
7. A compound spray disinfectant for farms according to claim 6, characterized in that: In the preparation method of the bactericidal synergistic stabilizer, the carboxylation degree of the carboxymethyl chitosan in step S3.1 is 80-90%.
8. The compound spray disinfectant for farms according to claim 6, characterized in that: In the preparation method of the bactericidal synergistic stabilizer, the deionized water dialysis time in step S3.2 is 5-6 days, and the deionized water is replaced every 10-12 hours during the dialysis period.
9. A process for preparing the compound spray disinfectant for farms according to any one of claims 1 to 8, characterized in that: The following steps are involved: A bactericidal synergistic stabilizer and a traditional Chinese medicine bactericidal composition are added to distilled water and stirred to disperse evenly, then a lignin-based electrostatic generator is added, and the mixture is placed in a cell crusher and ultrasonically treated at an ultrasonic frequency of 25-30 kHz for 20-25 minutes to obtain a water dispersant, methyl oleate is dripped into the water dispersant, and then the mixture is transferred to a cell crusher and treated at an ultrasonic frequency of 35-40 kHz for 12-15 minutes to obtain a compound spray disinfectant.