Anti-aging composition for reducing stress-type fatigue and preparation method and application thereof

Abstract

The application relates to the cosmetic technical field, and particularly discloses an anti-aging composition for reducing stress fatigue, a preparation method and application thereof. The anti-aging composition comprises a camellia chrysantha extract, a prosopis juliflora seed extract, a mu-conotoxin and an ascorbic acid polypeptide; the mass ratio of the camellia chrysantha extract, the prosopis juliflora seed extract, the mu-conotoxin and the ascorbic acid polypeptide is (0.1-5):(0.01-2):(0.001-1):(0.01-5). The anti-aging composition provided by the application can reduce stress damage of skin cells, improve cell vitality, thereby improving the problems of skin dullness, roughness, looseness and fine lines caused by overwork, work and emotional stress, and is mild and non-irritating, and is suitable for sensitive skin people.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and in particular to an anti-aging composition for reducing stress-induced fatigue, its preparation method, and its application. Background Technology

[0002] Under the heavy pressure of a fast-paced life, the skin suffers far more internal and external aggressors than can be seen with the naked eye, forming a typical "stressed skin" state. When staying up late deprives cells of their golden period for nighttime repair, the accumulation of metabolic waste and sluggish microcirculation cause the skin to lose its natural radiance, presenting a stubborn dullness. When work fatigue continuously triggers a cortisol storm, collagen and elastin fibers are rapidly consumed, and the subcutaneous support structure collapses like a ship without anchor, resulting in sagging and drooping facial contours. When emotional stress repeatedly impacts the skin barrier, nerve endings continuously release inflammatory signals, and the stratum corneum's ability to retain moisture breaks down, turning what were originally subtle facial expressions into persistent, fragile fine lines, ultimately weaving together a complex state of roughness, sagging, and withered fatigue. The essence of this "stressed skin" is the long-term consequence of multiple factors such as sleep deprivation, metabolic disorders, collagen loss, and a weakened skin barrier eroding cell vitality.

[0003] However, current mainstream anti-aging technologies are still mainly designed for natural aging mechanisms, making it difficult to meet the core needs of stress-induced skin aging for multi-pathway synergistic repair and gentleness. For example, in the selection of targets, there is an overemphasis on a single pathway that promotes collagen synthesis, while neglecting the reduced cell vitality, metabolic accumulation, and chronic inflammation in stress-induced aging; in the application of active ingredients, traditional anti-wrinkle ingredients such as retinol compounds can improve photoaging wrinkles, but their strong exfoliating effect can further damage the already compromised barrier integrity of stressed skin. Summary of the Invention

[0004] The purpose of this application is to overcome the shortcomings of the prior art and provide an anti-aging composition that reduces stress-induced fatigue, as well as its preparation method and application. The anti-aging composition provided by this application improves skin dullness, roughness, sagging, and fine lines caused by staying up late, work, and emotional stress by reducing stress-induced damage to skin cells and enhancing cell vitality. Furthermore, it is gentle and non-irritating, making it suitable for people with sensitive skin.

[0005] To achieve the above objectives, the technical solution adopted in this application is as follows:

[0006] This application provides an anti-aging composition comprising Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid polypeptide;

[0007] The mass ratio of the Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid polypeptide is (0.1–5):(0.01–2):(0.001–1):(0.01–5).

[0008] Through the above technical solution, four active components—Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid peptides—are used to penetrate and form a cascaded anti-aging network, comprehensively improving dullness, roughness, sagging, and fine lines caused by fatigue and stress. Specifically, Camellia chrysantha extract is rich in tea polyphenols and flavonol glycosides, which stimulate collagen synthesis and provide antioxidant protection, protecting skin cells from stress damage and significantly improving skin sagging. Edamame seed extract is rich in polysaccharides and flavonoid glycosides, which soothe skin stress by lowering the level of the stress hormone cortisol. μ-conospirin is a neuromodulatory peptide that significantly improves expression lines and fine lines by inhibiting muscle contraction signaling pathways and reducing dynamic muscle tension. Ascorbic acid peptides are a complex of vitamin C and peptides that significantly improve dullness, roughness, and signs of aging through powerful antioxidant, whitening, and collagen regeneration effects. Through extensive research and experimentation, the inventors of this application discovered that Camellia chrysantha extract and Edamame seed extract have a synergistic effect in promoting penetration. The polyphenolic compounds in Camellia chrysantha extract enhance lipid fluidity by interfering with the lipid arrangement of skin cells, thereby promoting the penetration of lipophilic components. The polysaccharides in Edamame seed extract increase skin hydration, forming a hydrophilic gel layer that enhances the hydration of the stratum corneum and reduces the diffusion resistance of hydrophilic compounds. The flavonoids in Camellia chrysantha extract and Edamame seed extract expand intercellular tight junctions by affecting the expression of tight junction proteins, thereby assisting the penetration of macromolecular peptides.

[0009] Furthermore, this application achieves a breakthrough intervention in stress-induced skin aging through the following comprehensive advantages: At the mechanistic level, it creatively constructs a multi-target synergistic pathway—flavonoid glycosides in soybean seed extract directly downregulate cortisol levels, blocking collagen degradation and barrier damage induced by stress hormones from the source; at the same time, camellia extract and ascorbic acid peptides form a dual-dimensional antioxidant matrix, synergistically neutralizing reactive oxygen species (ROS) generated by staying up late and emotional stress, and promoting collagen regeneration; while μ-conospirin precisely inhibits calcium ion channels of motor neurons, alleviating dynamic stretch fixation caused by continuous contraction of facial muscles. The four components of camellia extract, soybean seed extract, μ-conospirin, and ascorbic acid peptides form a cascade anti-aging network of "stress blocking-oxidative scavenging-collagen remodeling-muscle relaxation".

[0010] In terms of transdermal absorption of active ingredients, the polyphenols in Camellia chrysantha extract enhance cell membrane lipid fluidity, the hydration gel layer formed by Edamame seed polysaccharide improves stratum corneum permeability, and the flavonoids of both significantly optimize the transdermal efficiency of active ingredients such as μ-conospirin and ascorbic acid peptides through a triple mechanism that synergistically regulates tight junction protein expression to expand intercellular spaces. In terms of safety, the active ingredients avoid traditional strong irritants (such as retinol and high-concentration acids) by combining natural plant extracts (Camellia chrysantha / Edamame seed) with targeted modified peptides, making it suitable for people with sensitive skin.

[0011] Golden camellia extract, edamame seed extract, μ-cono-peptide, and ascorbic acid peptide, in the above-mentioned mass ratios, can reduce skin cell stress damage and enhance cell vitality, thereby improving skin dullness, roughness, sagging, and fine lines caused by staying up late, work, and emotional stress. It is also gentle and non-irritating, making it suitable for people with sensitive skin.

[0012] As a preferred embodiment of the anti-aging composition described in this application, the mass ratio of the Camellia chrysantha extract, Edamame seed extract, μ-cono-peptide and ascorbic acid polypeptide is (0.5-3):(0.05-0.5):(0.01-0.5):(0.1-2).

[0013] In the technical solution of this application, the golden camellia extract, edamame seed extract, μ-conospirin, and ascorbic acid peptide are used in the above-mentioned preferred mass ratio range. The golden camellia extract and edamame seed extract can better synergistically enhance the penetration of μ-conospirin and ascorbic acid peptide, thereby forming a better "stress blocking-oxidative scavenging-collagen remodeling-muscle relaxation" cascade anti-aging network with μ-conospirin and ascorbic acid peptide, comprehensively improving dullness, roughness, sagging, and fine lines caused by fatigue and stress. Furthermore, the golden camellia extract and ascorbic acid peptide form a two-dimensional antioxidant matrix, synergistically neutralizing reactive oxygen species (ROS) generated by staying up late and emotional stress, and promoting collagen regeneration, improving sagging and fine lines caused by fatigue and stress.

[0014] As a preferred embodiment of the anti-aging composition described in this application, the mass ratio of the Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid polypeptide is (1-2):(0.2-0.3):(0.1-0.2):(0.6-0.8).

[0015] When the mass ratio of Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid peptide in this application is more preferably within the above range, it can better form a cascade anti-aging network of "stress blocking-oxidation scavenging-collagen remodeling-muscle relaxation", comprehensively improving dullness, roughness, sagging, and fine lines caused by fatigue and stress.

[0016] In this application, the extract of Camellia chrysantha and ascorbic acid peptides form a two-dimensional antioxidant matrix, which synergistically neutralizes reactive oxygen species (ROS) generated by staying up late and emotional stress, and promotes collagen regeneration. The synergistic combination of the above components can better stimulate collagen synthesis and improve the antioxidant effect. When applied to anti-aging compositions, it can better protect skin cells from stress damage, significantly improve skin sagging, and achieve anti-aging effects.

[0017] As a preferred embodiment of the anti-aging composition described in this application, the method for preparing the grey soybean seed extract includes the following steps:

[0018] 1) Add the gray soybean seed powder to the enzymatic hydrolysate for enzymatic hydrolysis, freeze-dry, and obtain the reactant;

[0019] 2) The reactants were extracted by supercritical CO2 to obtain edamame seed extract;

[0020] The conditions for supercritical CO2 extraction include: extraction pressure of 30–35 MPa, extraction temperature of 50–60 °C, extraction time of 1.5–3 h, separation pressure of 5.5–6.5 MPa, separation temperature of 35–45 °C, and CO2 flow rate of 15–30 L / h.

[0021] This application employs an enzymatic pretreatment combined with supercritical CO2 extraction. While preserving the activity of soybean seed polysaccharides and flavonoid glycosides, it enhances the dissolution rate of target components by precisely breaking down cell walls. At the same time, it utilizes the inertness and low-temperature properties of supercritical CO2 to achieve a green extraction process with no organic solvent residue and low allergenicity, which is significantly superior to traditional organic solvent or high-temperature water extraction methods, and is especially suitable for the high purity and safety requirements of skin care raw materials.

[0022] Compared to other methods, the grey soybean seed extract prepared by the above method is richer in polysaccharides and flavonoid glycosides. When combined with the other three components, it forms an anti-aging composition. When applied to skin care, it can reduce the level of the stress hormone cortisol, thereby blocking the collagen degradation and barrier damage induced by stress hormones from the source and relieving skin stress.

[0023] Among them, the extract of grey soybean seed and ascorbic acid peptide work synergistically to better improve cell vitality and improve skin dullness, roughness, sagging and fine lines caused by staying up late, work and emotional stress.

[0024] In some specific embodiments, the gray soybeans are peeled, the seeds are removed, dried, pulverized, and sieved to obtain gray soybean seed powder.

[0025] In a preferred embodiment of the anti-aging composition described in this application, the weight ratio of soybean seed powder to enzymatic hydrolysate in step 1) is 1:(5-15).

[0026] Using the above weight ratio range for soybean seed powder and enzymatic hydrolysate can yield more polysaccharides and flavonoid glycosides, thus improving the dissolution rate of the target components.

[0027] More preferably, in step 1), the weight ratio of soybean seed powder to enzymatic hydrolysate is 1:(8-12).

[0028] The above-mentioned weight ratio of edamame seed powder and enzymatic hydrolysate is preferred to obtain edamame seed extract of better quality.

[0029] As a preferred embodiment of the anti-aging composition described in this application, in step 1), the enzymatic hydrolysate includes 2.5 million to 5 million U / L cellulase and 800,000 to 1.2 million U / L hemicellulase, and the pH value of the enzymatic hydrolysate is 5.0 to 5.5.

[0030] In a preferred embodiment of the anti-aging composition described in this application, the enzymatic hydrolysis temperature in step 1) is 45-55°C, and the enzymatic hydrolysis time is 1-2 hours.

[0031] In the technical solution of this application, the enzymatic hydrolysate contains the above-mentioned cellulase and hemicellulase, which enzymatically hydrolyze the gray soybean seed powder. Combined with the supercritical CO2 method, more polysaccharides and flavonoid glycosides are obtained, which can better improve skin hydration, form a hydrophilic gel layer to improve the hydration of the stratum corneum, reduce the diffusion resistance of hydrophilic compounds, and promote the penetration of μ-conospirin and ascorbic acid peptides, thereby enhancing the anti-aging effect of the composition.

[0032] Through the above technical solutions, the polyphenolic compounds in Camellia chrysantha extract enhance lipid fluidity and promote the penetration of lipophilic components by interfering with the lipid arrangement of skin cells; the polysaccharides in Edamame seed extract improve skin hydration, form a hydrophilic gel layer to enhance the hydration of the stratum corneum, and reduce the diffusion resistance of hydrophilic compounds; Camellia chrysantha extract and Edamame seed extract have a synergistic penetration-enhancing effect, and the flavonoids in Camellia chrysantha extract and Edamame seed extract expand intercellular tight junctions by affecting the expression of tight junction proteins, assisting the penetration of macromolecular peptides, thereby improving the utilization rate of μ-conospiropeptide and ascorbic acid peptides, and thus exerting the corresponding anti-aging effect.

[0033] This application also provides the use of the above-mentioned anti-aging composition in the preparation of products that reduce skin cell stress damage.

[0034] The anti-aging composition provided in this application can reduce skin cell stress damage and enhance cell vitality, thereby improving skin dullness, roughness, sagging and fine lines caused by staying up late, work and emotional stress. It is also gentle and non-irritating, making it suitable for people with sensitive skin.

[0035] This application also provides the use of the above-mentioned anti-aging composition in the preparation of brightening, anti-aging, repairing or anti-sensitivity skin care products.

[0036] Preferably, the skincare product includes at least one of toner, lotion, cream, mask, serum, and spray.

[0037] This application also provides an anti-aging skincare product, which includes a lotion;

[0038] The emulsion comprises the following components by weight percentage:

[0039] Thickener 0.05-0.3%, humectant 0.5-3%, pH adjuster 0.01-0.3%, emulsifier 0.5-5%, preservative 0.5-3%, anti-aging composition 1-5%.

[0040] As a preferred embodiment of the anti-aging skin care product described in this application, the thickener includes at least one of the following: polyacrylate crosspolymer-6, carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, argan gum, guar gum, ammonium acryloyl dimethyl taurate / VP copolymer, acrylate copolymer, and sclerotium gum.

[0041] The moisturizer includes at least one of the following: glycerin, D-panthenol, vitamin B5, 1,2-butanediol, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, caprylyl glycol, dipropylene glycol, tremella polysaccharide, trehalose, betaine, allantoin, sodium hyaluronate, β-glucan, budding stalk polysaccharide, and ceramide.

[0042] The pH adjuster includes at least one of arginine, tromethamine, and disodium EDTA.

[0043] The emulsifier includes at least one of the following: caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, sodium polyacrylate, hydrogenated lecithin, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate.

[0044] The preservative includes at least one of p-hydroxyacetophenone and polyols.

[0045] By using the above technical solutions and adding the above types of thickeners, the viscosity of the product can be better adjusted, making the texture of the skin care product more stable, enhancing the product's viscosity and improving its feel.

[0046] Adding these types of moisturizers helps to absorb and retain moisture, reducing skin moisture loss and providing long-lasting hydration. This improves skin's softness and elasticity, and also reduces the appearance of fine lines and wrinkles.

[0047] Adding the above types of pH adjusters can maintain the product's acid-base balance, ensure formula stability, and avoid skin irritation; adding the above types of emulsifiers can promote oil-water mixing, form a stable emulsion structure, and make cosmetics easier to apply and have a more even texture.

[0048] Compared with the prior art, this application has the following beneficial effects:

[0049] This application provides an anti-aging composition for reducing stress-induced fatigue, its preparation method, and its application. The anti-aging composition contains Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid peptides. This application creatively constructs a multi-target synergistic pathway—flavonoid glycosides in Edamame seed extract directly downregulate cortisol levels, blocking stress hormone-induced collagen degradation and barrier damage at the source; simultaneously, Camellia chrysantha extract and ascorbic acid peptides form a dual-dimensional antioxidant matrix, synergistically neutralizing reactive oxygen species (ROS) generated by staying up late and emotional stress, and promoting collagen production. The formula promotes skin cell regeneration; while μ-conospirin precisely inhibits calcium ion channels in motor neurons, alleviating dynamic wrinkle fixation caused by sustained contraction of facial muscles. These four components form a cascade anti-aging network of "stress blocking - oxidative scavenging - collagen remodeling - muscle relaxation." Furthermore, the polyphenols in Camellia chrysantha extract enhance cell membrane lipid fluidity, the hydrating gel layer formed by Edamame seed polysaccharides improves stratum corneum permeability, and the flavonoids in both significantly regulate tight junction protein expression to expand intercellular spaces. This triple mechanism significantly optimizes the transdermal efficiency of active ingredients such as μ-conospirin and ascorbic acid peptides. The anti-aging composition provided in this application improves skin dullness, roughness, sagging, and fine lines caused by staying up late, work, and emotional stress by reducing skin cell stress damage and enhancing cell vitality. It is also gentle and non-irritating, making it suitable for sensitive skin. Attached Figure Description

[0050] Figure 1 The images show the results of improved gloss and skin texture before and after 28 days of use for Application Example 7 and the blank application example. Detailed Implementation

[0051] To better illustrate the purpose, technical solution, and advantages of this application, the following description will be provided in conjunction with the accompanying drawings and specific embodiments.

[0052] In the following examples and comparative examples, unless otherwise specified, the experimental methods used are conventional methods, and the materials and reagents used are commercially available unless otherwise specified. Furthermore, the raw materials used in each parallel experiment are the same.

[0053] Unless otherwise specified, all raw materials used in the embodiments and comparative examples in this application are commercially available products, specifically:

[0054] μ-conopeptide: purchased from Shenzhen Weiqi Biotechnology Co., Ltd.;

[0055] Ascorbic acid peptide: purchased from BioHarvest Sciences, Canada;

[0056] Golden Camellia extract: purchased from Ximuyuan (Xiamen) Biotechnology Co., Ltd.;

[0057] Honeysuckle extract: purchased from Ningbo (Xi'an) Biomedical Technology Co., Ltd.;

[0058] Soybean extract: purchased from Shanxi Kangrui Kejian Biotechnology Co., Ltd.;

[0059] Edamame seed extract 1, homemade, including the following steps:

[0060] 1) Peel the soybeans and remove the seeds. Dry them, crush them, and sieve them to obtain soybean seed powder. Add the powder to an enzymatic hydrolysate (which contains 3.75 million U / L cellulase and 1 million U / L hemicellulase, with a pH of 5.2) to carry out the enzymatic hydrolysis reaction. The weight ratio of soybean seed powder to enzymatic hydrolysate is 1:10. The hydrolysis temperature is 50℃ and the hydrolysis time is 1.5 hours. Freeze-dry the mixture to obtain the reactants.

[0061] 2) The reactants were extracted by supercritical CO2 to obtain edamame seed extract;

[0062] The conditions for supercritical CO2 extraction include: extraction pressure of 33 MPa, extraction temperature of 55℃, extraction time of 2 h, separation pressure of 6.0 MPa, separation temperature of 40℃, and CO2 flow rate of 25 L / h.

[0063] Edamame seed extract 2, homemade, including the following steps:

[0064] 1) Peel the soybeans and remove the seeds. Dry them, then crush and sieve them to obtain soybean seed powder. Add the powder to an enzymatic hydrolysate (which contains 2.5 million U / L cellulase and 800,000 U / L hemicellulase, with a pH of 5) to carry out the enzymatic hydrolysis reaction. The weight ratio of soybean seed powder to enzymatic hydrolysate is 1:5. The hydrolysis temperature is 45℃ and the hydrolysis time is 1 hour. Freeze-dry the mixture to obtain the reactants.

[0065] 2) The reactants were extracted by supercritical CO2 to obtain edamame seed extract;

[0066] The conditions for supercritical CO2 extraction include: extraction pressure of 30 MPa, extraction temperature of 50 °C, extraction time of 1.5 h, separation pressure of 5.5 MPa, separation temperature of 35 °C, and CO2 flow rate of 15 L / h.

[0067] Grey soybean seed extract 3, homemade, including the following steps:

[0068] 1) Peel the soybeans and remove the seeds. Dry them, crush them, and sieve them to obtain soybean seed powder. Add the powder to an enzymatic hydrolysate (which contains 5 million U / L cellulase and 1.2 million U / L hemicellulase, with a pH of 5.5) to carry out the enzymatic hydrolysis reaction. The weight ratio of soybean seed powder to enzymatic hydrolysate is 1:15. The hydrolysis temperature is 55℃ and the hydrolysis time is 5 hours. Freeze-dry the mixture to obtain the reactants.

[0069] 2) The reactants were extracted by supercritical CO2 to obtain edamame seed extract;

[0070] The conditions for supercritical CO2 extraction include: extraction pressure of 35 MPa, extraction temperature of 60℃, extraction time of 3 h, separation pressure of 6.5 MPa, separation temperature of 45℃, and CO2 flow rate of 30 L / h.

[0071] 4g of grey soybean seed extract, homemade, including the following steps:

[0072] 1) Peel the soybeans and remove the seeds. Dry them, then crush and sieve them to obtain soybean seed powder. Add the powder to an enzymatic hydrolysate (containing 3.75 million U / L cellulase and 1 million U / L hemicellulase, with a pH of 5.2) for enzymatic hydrolysis. The weight ratio of soybean seed powder to enzymatic hydrolysate is 1:8. The hydrolysis temperature is 50℃ and the hydrolysis time is 1.5 hours. Freeze-dry to obtain the reactants.

[0073] 2) The reactants were extracted by supercritical CO2 to obtain edamame seed extract;

[0074] The conditions for supercritical CO2 extraction include: extraction pressure of 33 MPa, extraction temperature of 55℃, extraction time of 2 h, separation pressure of 6.0 MPa, separation temperature of 40℃, and CO2 flow rate of 25 L / h.

[0075] 5g of grey soybean seed extract, homemade, including the following steps:

[0076] 1) Peel the soybeans and remove the seeds. Dry them, crush them, and sieve them to obtain soybean seed powder. Add the powder to an enzymatic hydrolysate (which contains 3.75 million U / L cellulase and 1 million U / L hemicellulase, with a pH of 5.2) to carry out the enzymatic hydrolysis reaction. The weight ratio of soybean seed powder to enzymatic hydrolysate is 1:12. The hydrolysis temperature is 50℃ and the hydrolysis time is 1.5 hours. Freeze-dry the mixture to obtain the reactants.

[0077] 2) The reactants were extracted by supercritical CO2 to obtain edamame seed extract;

[0078] The conditions for supercritical CO2 extraction include: extraction pressure of 33 MPa, extraction temperature of 55℃, extraction time of 2 h, separation pressure of 6.0 MPa, separation temperature of 40℃, and CO2 flow rate of 25 L / h.

[0079] Grey soybean seed extract 6, homemade, including the following steps:

[0080] 1) Peel the soybeans and remove the seeds. Dry them, crush them, and sieve them to obtain soybean seed powder. Add the powder to an enzymatic hydrolysate (which contains 3.75 million U / L cellulase and 1 million U / L hemicellulase, with a pH of 5.2) to carry out the enzymatic hydrolysis reaction. The weight ratio of soybean seed powder to enzymatic hydrolysate is 1:10. The hydrolysis temperature is 50℃ and the hydrolysis time is 1.5 hours. Freeze-dry the mixture to obtain the reactants.

[0081] 2) The reactants were extracted by supercritical CO2 to obtain edamame seed extract;

[0082] The conditions for supercritical CO2 extraction include: extraction pressure of 20 MPa, extraction temperature of 45℃, extraction time of 2 h, separation pressure of 4.5 MPa, separation temperature of 25℃, and CO2 flow rate of 40 L / h.

[0083] Edamame seed extract 7, homemade, including the following steps:

[0084] After peeling the edamame, the seeds were dried, crushed, and sieved to obtain edamame seed powder. The powder was then extracted using supercritical CO2 to obtain edamame seed extract.

[0085] The conditions for supercritical CO2 extraction include: extraction pressure of 33 MPa, extraction temperature of 55℃, extraction time of 2 h, separation pressure of 6.0 MPa, separation temperature of 40℃, and CO2 flow rate of 25 L / h.

[0086] Compared to soybean seed extract 1, soybean seed extract 7 did not undergo enzymatic hydrolysis.

[0087] 8g of grey soybean seed extract, homemade, including the following steps:

[0088] 1) Peel the soybeans and remove the seeds. Dry them, then crush and sieve them to obtain soybean seed powder. Add the powder to an enzymatic hydrolysate (which contains 3.75 million U / L pectinase and 1 million U / L papain, with a pH of 5.2) for enzymatic hydrolysis. The weight ratio of soybean seed powder to hydrolysate is 1:10. The hydrolysis temperature is 50℃ and the hydrolysis time is 1.5 hours. Freeze-dry the mixture to obtain the reactants.

[0089] 2) The reactants were extracted by supercritical CO2 to obtain edamame seed extract;

[0090] The conditions for supercritical CO2 extraction include: extraction pressure of 33 MPa, extraction temperature of 55℃, extraction time of 2 h, separation pressure of 6.0 MPa, separation temperature of 40℃, and CO2 flow rate of 25 L / h.

[0091] Grey soybean seed extract 9, homemade, including the following steps:

[0092] After peeling the soybeans, the seeds were dried, pulverized, and sieved to obtain soybean seed powder. The powder was then added to an ethanol solution (20% by mass) for ultrasonic extraction at 50°C for 30 minutes at 120W with a solid-liquid ratio of 1g:10mL. After ultrasonic extraction, the supernatant was collected by centrifugation and dried to obtain soybean seed extract.

[0093] Examples 1-15 and Comparative Examples 1-9: An anti-aging composition

[0094] Examples 1-15 and Comparative Examples 1-9 provide an anti-aging composition, the formulation of which is shown in Table 1 (parts by weight).

[0095] Table 1

[0096]

[0097]

[0098]

[0099] Comparative Example 10

[0100] Compared with Example 7, the difference in Comparative Example 10 is that honeysuckle extract is used instead of camellia extract, while the other components and quantities are the same as in Example 7.

[0101] Comparative Example 11

[0102] Compared with Example 7, the difference in Comparative Example 11 is that soybean extract is used instead of edamame seed extract, while the other components and quantities are the same as in Example 7.

[0103] Application Examples 1-17 and Comparative Application Examples 1-11

[0104] Application Examples 1-17 and Comparative Application Examples 1-11 provide an anti-aging emulsion. The raw material components and their mass percentages are shown in Table 2. Although Table 2 illustrates the anti-aging compositions used in the corresponding examples / comparative examples, for clarity, it is recommended to illustrate them here by way of example. For instance, Application Example 1 uses the anti-aging composition of Example 1, Application Example 2 uses the anti-aging composition of Example 2, and so on. Comparative Application Example 1 uses the anti-aging composition of Comparative Example 1, Comparative Application Example 2 uses the anti-aging composition of Comparative Example 2, and so on. Application Examples 16-17 use the anti-aging composition of Example 7.

[0105] Table 2

[0106]

[0107] This application provides a method for preparing an anti-aging emulsion, the method comprising the following steps:

[0108] (1) Mix the humectant, thickener and pH adjuster with water and stir. Heat to 85°C and homogenize at 1300 rpm for 4 minutes. After homogenization, keep warm for later use to obtain pre-prepared component A.

[0109] (2) Mix the emulsifier, heat to 75°C, and homogenize at 1300 rpm for 4 min. After homogenization, keep warm for later use to obtain the pre-prepared component B.

[0110] (3) Mix the preservatives and heat to 60°C to melt them to obtain pre-prepared component C;

[0111] (4) Heat the pre-prepared component A to 80°C, add the pre-prepared component B at 300 rpm, stir and mix, then cool down to 60°C, add the pre-prepared component C at 300 rpm and stir and mix, then cool down to below 45°C, add the anti-aging composition and continue stirring for 8 minutes, finally add the remaining pH adjuster (arginine) to adjust the pH to 5.5-7.0, then stop stirring, discharge the material, and obtain the anti-aging emulsion.

[0112] Experimental Example 1: Skin Permeability Test of Anti-aging Composition

[0113] Experimental method: Ex vivo pig skin was used for testing, and the exposed skin area in the diffusion cell was 1.5 cm². 2 The receiving chamber volume was 5.0 mL. A phosphate buffer solution with a pH of 5.8 and anhydrous ethanol at a volume ratio of 50:50 was used as the receiving solution. 200 μL of the anti-aging emulsions prepared in Application Examples 1-17 and Comparative Application Examples 1-11 were taken respectively, applied evenly to the skin, and covered with a thin film to prevent evaporation.

[0114] The initial total peptide content (M0) and the transdermal absorption (M1) of total peptides in the emulsion after 24 hours were determined using the BCA method. The peptide transdermal rate was calculated as M1 / M2. Specifically, 50 μL of sample was added to 1 mL of BCA working solution, reacted at 37°C for 30 min, and the absorbance (OD) at 562 nm was measured using a UV spectrophotometer. The total peptide content of the sample was calculated by referring to the standard curve of bovine serum albumin (BSA). A higher 24-hour peptide transdermal rate indicates better skin permeability of the anti-aging composition. The results are shown in Table 3 below.

[0115] Table 3

[0116]

[0117]

[0118] Conclusion: The above experiments show that the 24-hour peptide transdermal penetration rate of the anti-aging emulsions prepared in Examples 1-17 was 48%-86.4%. Among them, the skin permeability of the anti-aging compositions in Examples 2 and 5 was better than that in Examples 1 and 6, indicating that the optimal mass ratio of Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid peptide was (0.5-3):(0.05-0.5):(0.01-0.5):(0.1-2) to improve skin permeability. The skin permeability of the anti-aging compositions in Examples 3-4 was better than that in Examples 2 and 5, indicating that the optimal mass ratio of Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid peptide was (1-2):(0.2-0.3):(0.1-0.2):(0.6-0.8) to further improve skin permeability. The anti-aging composition in Example 7 showed the best skin permeability.

[0119] When different mass percentages of the anti-aging composition were added to the anti-aging emulsions in Examples 16 and 17, the skin permeability of the anti-aging emulsions varied, indicating that the amount of anti-aging composition added has a significant impact on skin permeability.

[0120] The 24-hour peptide transdermal penetration rate of the anti-aging emulsions prepared in Examples 7-11 was 80.9%-86.4%. The anti-aging emulsions in Examples 10-11 showed better skin permeability than those in Examples 8-9. This indicates that a weight ratio of 1:(8-12) of edamame seed powder to enzymatic hydrolysate in the preparation of edamame seed extract promotes better skin permeability than a weight ratio of 1:(5-15).

[0121] Compared to Application Examples 7-11 and Application Examples 12-14, when the extraction pressure, extraction temperature, and extraction time used in the preparation of the *Edamame oleracea* seed extract in the anti-aging composition of Application Example 12 were outside the scope of this application (extraction pressure of 30-35 MPa, extraction temperature of 50-60°C, and extraction time of 1.5-3 h), the preparation process of the *Edamame oleracea* seed extract in the anti-aging composition of Application Example 13 did not involve enzymatic hydrolysis, and the preparation process of the *Edamame oleracea* seed extract in the anti-aging composition of Application Example 14 used other types of enzymatic hydrolysates, Application Examples 7-11 yielded more *Edamame oleracea* seed polysaccharides and flavonoids, and Application Examples 12-14 were less effective than Application Examples 7-9 in improving skin hydration, reducing diffusion resistance of hydrophilic compounds, and promoting peptide penetration. Compared to Application Example 15, when the *Edamame oleracea* seed extract was prepared using other types of methods (ultrasonic extraction has low extraction efficiency), the skin permeability of the resulting anti-aging composition was poor.

[0122] Compared to application examples 1-3, which did not contain Camellia chrysantha extract and / or Edamame seed extract, and application examples 10-11, which used other types of extracts to replace Camellia chrysantha extract or Edamame seed extract, the 24-hour peptide transdermal permeability was lower, indicating poor skin penetration of the anti-aging composition. This suggests that Camellia chrysantha extract and Edamame seed extract have a synergistic penetration-enhancing effect. The polyphenolic compounds in Camellia chrysantha extract enhance lipid fluidity by interfering with the lipid arrangement of skin cells, thus promoting the penetration of lipophilic components. The polysaccharides in Edamame seed extract increase skin hydration, forming a hydrophilic gel layer that enhances stratum corneum hydration and reduces the diffusion resistance of hydrophilic compounds. The flavonoids in Camellia chrysantha extract and Edamame seed extract expand intercellular tight junctions by affecting the expression of tight junction proteins, thus facilitating the penetration of macromolecular peptides.

[0123] Compared with Application Examples 6 and 7, when the amount of anti-aging composition was kept constant, removing the Camellia chrysantha extract or Edamame seed extract alone resulted in lower skin permeability of the anti-aging composition compared to the anti-aging composition used in Application Example 7.

[0124] In comparative application examples 8-9, the mass ratio of Camellia chrysantha extract and Edamame seed extract was outside the scope of this application, resulting in poor skin penetration of the anti-aging composition.

[0125] Experimental Example 2: Effects of Anti-aging Compositions on Skin Cells under Conditions of Staying Up Late / Fatigue and Stress

[0126] Lack of sleep and fatigue accelerate skin aging through a dual mechanism of inducing oxidative stress and elevated cortisol levels. During prolonged periods of staying up late, reactive oxygen species (ROS) accumulate in large quantities, directly attacking the mitochondrial function of dermal fibroblasts, leading to a significant decrease in cell vitality. Simultaneously, stress activates the hypothalamic-pituitary-adrenal axis, causing abnormally high cortisol secretion. Cortisol, by binding to glucocorticoid receptors, inhibits collagen production and activates matrix metalloproteinases (MMPs), accelerating collagen degradation. This synergistic effect of oxidative damage and cortisol creates a "double whammy" of cellular dysfunction, resulting in decreased skin cell vitality, reduced collagen, and ultimately, damage to the dermal matrix structure, loss of skin elasticity, and deepening wrinkles—signs of aging.

[0127] The cell line used in this experiment was human dermal fibroblast HDF (catalog number PC-202h, Wuhan Saisuo Biotechnology Co., Ltd.). The test conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to groups, followed by tests, specifically cell viability testing and type I collagen content detection. The test methods are as follows:

[0128] (1) The cell suspension was seeded into a 96-well cell culture plate at a density of 2000 cells / well. 100 μL of DMEM high glucose culture medium (Gibco) containing 10% fetal bovine serum was added to each well and cultured for 24 h.

[0129] (2) Discard the supernatant. Add 100 μL of DMEM medium (2105341, Gibco) to the blank control group. Add 100 μL of DMEM medium containing 200 μM hydrogen peroxide (Maclean, H792073) and 150 μM cortisol (Shanghai Yuanye Biotechnology, S31360) to the model group. Add 100 μL of medium containing 0.5 wt% of the corresponding anti-aging composition (the anti-aging compositions prepared in Examples 1-15 and Comparative Examples 1-11) and 200 μM hydrogen peroxide and 150 μM cortisol to the sample group. Repeat each group in 6 wells and incubate for 24 h.

[0130] (3) Cell viability assay: The cell viability of each group was detected using the CCK8 cell proliferation kit (Catalog No. C6005M, Suzhou Youyilandi Biotechnology Co., Ltd.). The specific operation was to add 10 μL of CCK8 solution to each well, continue to incubate at 37℃ for 2 h, shake for 30 s using an ELISA reader, and then detect the absorbance OD value at a wavelength of 450 nm using an ELISA reader.

[0131] (4) Detection of type I collagen content: The type I collagen content of each group was detected using a human type I collagen (Col I) ELISA kit (catalog number ml057630, Shanghai Enzyme-Linked Biotechnology). The specific operation was as follows: cells of each group were collected, the supernatant was discarded, and the cells were lysed with RIPA lysis buffer containing protease inhibitor (Shanghai Merck, V900854). The lysed samples were centrifuged at 12000g for 5 minutes, and the supernatant was collected. Then, according to the kit instructions, equal amounts of supernatant were added to the corresponding reaction wells of the kit. After incubation, washing, and color development, the absorbance OD value was measured at a specific wavelength using an ELISA reader. The type I collagen content of each group was calculated according to the standard curve.

[0132] The effect of the composition on improving cell vitality is represented by the improvement in cell survival, and the anti-aging effect is represented by the improvement in type I collagen content. The calculation method is as follows:

[0133] Improvement rate = (OD value) 样品组 -OD value 模型组 ) / OD value 模型组 ×100%; the results are shown in Table 4.

[0134] Table 4

[0135] Group Cell survival improvement rate / % Improvement rate of type I collagen content / % Example 1 37.6 88.8 Example 2 41.3 117.4 Example 3 58.5 147.4 Example 4 58.5 144.9 Example 5 47.2 110.5 Example 6 38.9 103.7 Example 7 63.9 157.3 Example 8 59.1 152.7 Example 9 59.2 152.3 Example 10 61.6 154.9 Example 11 62.3 155.7 Example 12 48.7 143.7 Example 13 50.9 144.6 Example 14 54.1 146.3 Example 15 42.5 111.8 Comparative Example 1 22.4 14.8 Comparative Example 2 16.5 60.7 Comparative Example 3 10.5 11.1 Comparative Example 4 31.9 65.2 Comparative Example 5 11.2 43.5 Comparative Example 6 17.2 63.2 Comparative Example 7 23.0 17.9 Comparative Example 8 35.8 73.8 Comparative Example 9 32.0 66.3 Comparative Example 10 25.1 15.3 Comparative Example 11 8.4 66.2

[0136] Conclusion: The cell survival improvement rate of the anti-aging compositions prepared in Examples 1-15 was 37.6%-63.9%, and the improvement rate of type I collagen content was 88.8%-157.3%, indicating that the anti-aging compositions of this application can effectively improve cell vitality and anti-aging effects.

[0137] Among them, the anti-aging compositions of Examples 2 and 5 showed better cell vitality enhancement and anti-aging effects than those of Examples 1 and 6, indicating that the preferred mass ratio of Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid peptide is (0.5-3):(0.05-0.5):(0.01-0.5):(0.1-2), which can enhance cell vitality and anti-aging effects. The anti-aging compositions of Examples 3 and 4 showed better cell vitality enhancement and anti-aging effects than those of Examples 2 and 5, indicating that the mass ratio of Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid peptide is (1-2):(0.2-0.3):(0.1-0.2):(0.6-0.8), which can better enhance cell vitality and anti-aging effects. The anti-aging composition of Example 7 showed the best cell vitality enhancement and anti-aging effects.

[0138] The anti-aging compositions prepared in Examples 7-11 showed a cell survival improvement rate of 59.1%-63.9% and a type I collagen content improvement rate of 152.3%-157.3%. The anti-aging compositions in Examples 10-11 were superior to those in Examples 8-9 in terms of improving cell vitality and anti-aging effects. This indicates that the weight ratio of soybean seed powder to enzymatic hydrolysate of 1:(8-12) in the preparation of soybean seed extract is more effective in improving cell vitality and anti-aging effects than the weight ratio of soybean seed powder to enzymatic hydrolysate of 1:(5-15).

[0139] Compared with Examples 12-14, Examples 7-11 show that the extraction pressure, extraction temperature, and extraction time used in the preparation of the edamame seed extract in the anti-aging composition of Example 12 are not within the scope of this application (extraction pressure is 30-35 MPa, extraction temperature is 50-60°C, and extraction time is 1.5-3 h), the preparation of the edamame seed extract in the anti-aging composition of Example 13 does not contain enzymatic hydrolysis, and the preparation of the edamame seed extract in the anti-aging composition of Example 14 uses other types of enzymatic hydrolysates. Therefore, the cell survival improvement rate and type I collagen content improvement rate of Examples 12-14 are not as good as those of Examples 7-11.

[0140] When the extract of edamame seeds in Example 15 was not extracted using the supercritical CO2 method, the cell survival improvement rate and type I collagen content improvement rate of the obtained anti-aging composition were both lower than those in Examples 7-11.

[0141] Comparative Examples 1-3 did not contain Camellia chrysantha extract and / or Edamame seed extract. Comparative Examples 10-11 used other types of extracts to replace Camellia chrysantha extract or Edamame seed extract. The cell survival improvement rate and type I collagen content improvement rate of the anti-aging compositions were not as good as those of Example 7, indicating that Camellia chrysantha extract and Edamame seed extract can work synergistically to effectively improve cell vitality and anti-aging effects.

[0142] In Comparative Examples 6 and 7, while keeping the amount of anti-aging composition unchanged, removing the edamame seed extract alone resulted in an anti-aging composition with a lower cell vitality effect than the anti-aging composition used in Example 7; removing the camellia oleifera extract alone also resulted in an anti-aging composition with a lower anti-aging effect than the anti-aging composition used in Example 7.

[0143] The mass ratio of Camellia chrysantha extract and Edamame seed extract in Comparative Examples 8 and 9 is outside the scope of this application, and the cell vitality effect and anti-aging effect of the anti-aging composition are not as good as the anti-aging composition used in Example 7.

[0144] Experimental Example 3: Human Skin Patch Test

[0145] Sixty volunteers were recruited, 30 men and 30 women, aged 20-50 years, and randomly divided into two groups of 15 men and 15 women each. A closed patch assay was used. Equal volumes (0.020 mL–0.025 mL) of the test sample (emulsions prepared in Application Examples 1–17, Control Application Examples 1–11, and the blank application example) were placed in a specific patch applicator. The patch was applied to the volunteer's arm with hypoallergenic adhesive tape, and gently pressed to ensure even application to the skin. The patch was left on for 24 hours. The blank control group used distilled water, and the blank application examples used an emulsion without the anti-aging composition. One patch applicator was applied to each arm of each volunteer. Each patch applicator had 10 holes, allowing each volunteer (both arms) to test 20 samples simultaneously. The first group used emulsions prepared in Application Examples 1–17 and the blank application example, while the second group used emulsions prepared in Control Application Examples 1–11 and the blank application example. A blank control was included in each group. After 24 hours, the patch applicator was removed, and skin reactions were observed and recorded at 0.5 hours, 24 hours, and 48 hours. The severity of adverse skin reactions is shown in Table 5 below.

[0146] Table 5

[0147]

[0148] After testing, the anti-aging emulsions prepared in Application Examples 1-17 and Comparative Application Examples 1-11 of this invention showed negative reactions after human patch testing, indicating that they are safe and non-irritating to human skin.

[0149] Experimental Example 4: Human Efficacy Test of Anti-aging Composition in Brightening, Refining, Firming, and Reducing Wrinkles

[0150] Experimental methods:

[0151] 1. Experimental basis:

[0152] The human efficacy evaluation test method shall be followed in accordance with the "Technical Specifications for Cosmetic Safety" (2015 edition).

[0153] 2. Subject screening:

[0154] Inclusion criteria: We are recruiting Asian adult volunteers aged 25-40 who meet the following conditions:

[0155] a) Frequently stay up late (go to bed after 11 p.m. and have an average of less than 6 hours of sleep per day);

[0156] b) High work pressure (medical staff or those working in emerging internet industries, with an average daily working time of 9 hours or more);

[0157] c) A self-reported history of skin allergies (confirmed by a dermatologist);

[0158] d) No history of serious systemic diseases or skin diseases.

[0159] Exclusion criteria: pregnant / lactating women, individuals with severe allergies, and individuals who have participated in other clinical trials within the past 3 months.

[0160] Number of participants: A total of 174 qualified volunteers were included and divided into 29 groups of 6 people each using a random number table.

[0161] 3. Sample application method:

[0162] Test samples: Anti-aging emulsions prepared in Application Examples 1-17, Comparative Application Examples 1-11, and Blank Application Examples (double-blind method, numbered).

[0163] How to use: After cleansing in the morning and evening, volunteers should take 1mL of the sample and apply it evenly to the entire face, gently massaging until fully absorbed.

[0164] 4. Testing cycle and process:

[0165] Test period: 28 days (D0 to D28).

[0166] Visit time points: Instrument testing was conducted at D0 (baseline period) and D28 (end of the test).

[0167] Preparation before testing:

[0168] a) After the participants arrived, they used a uniform, non-irritating facial cleanser to clean their faces;

[0169] b) Rest for 30 minutes in a constant temperature and humidity environment (temperature 21±1℃, humidity 50±10%);

[0170] c) Keep your eyes closed and relax during the test to avoid facial expressions and movements that may interfere with the process.

[0171] 5. Testing instruments and test indicators:

[0172] Before testing, the instruments were calibrated to standard parameters. Researchers used the CK GL200 skin gloss test probe to measure the gloss of the cheekbones; a cutometer was used to measure the firmness (F4) of the cheekbones; and VISIA-CR was used to photograph and analyze the volunteers' skin texture and wrinkles. During the test, the probe pressure and measurement time were kept constant, and the same area was measured three times, and the average value was taken.

[0173] The brightening effect of the samples is represented by the improvement in luster; the skin firming effect is represented by the improvement in the firmness F4 value; the skin smoothing effect is represented by the improvement in the skin texture score; and the fine line reduction effect is represented by the improvement in the number of facial fine lines. The formulas are as follows:

[0174] Gloss improvement rate = (gloss value) T28 - Glossiness T0Gloss T0 ×100%;

[0175] Firmness F4 value improvement rate = (F4 T0 -F4 T28 ) / F4 T0 ×100%;

[0176] Skin texture score improvement rate = (skin texture score) T0 -Skin texture score T28 Skin texture score T0 ×100%;

[0177] Improvement rate of fine line quantity = (number of fine lines) T0 -Number of fine lines T28 ) / Number of fine lines T0 ×100%;

[0178] The results are shown in Table 6.

[0179] Table 6

[0180]

[0181]

[0182] Experiments showed that the anti-aging emulsions prepared using Examples 1-17 improved gloss by 7.9% to 16.9%; firmness F4 by 15.8% to 33.2%; skin texture by 33.9% to 63.1%; and the number of fine lines by 28.7% to 55.7%.

[0183] Among them, the anti-aging emulsions prepared by Application Examples 1 to 7 showed an improvement rate of 7.9% to 16.9% in gloss value, 15.8% to 33.2% in firmness F4 value, 33.9% to 63.1% in skin texture score, and 28.7% to 55.7% in the number of fine lines. The anti-aging emulsions prepared by Application Examples 1 to 7 can effectively improve gloss, firmness, skin texture, and fine lines. The anti-aging emulsions prepared by Application Examples 3 and 4 are better than those prepared by Application Examples 2 and 5 in improving gloss, firmness, skin texture, and fine lines. The anti-aging emulsions prepared by Application Examples 2 and 5 are better than those prepared by Application Examples 1 and 6 in improving gloss, firmness, skin texture, and fine lines. The anti-aging emulsion prepared by Application Example 7 shows the best improvement in gloss, firmness, skin texture, and fine lines.

[0184] In Application Examples 16-17, different mass percentages of anti-aging composition were added to the anti-aging emulsions. The anti-aging emulsions showed differences in improving radiance, firmness, skin texture, and fine lines, indicating that the amount of anti-aging composition added has a significant impact on improving radiance, firmness, skin texture, and fine lines.

[0185] The anti-aging emulsions prepared in Examples 7-11 showed better improvement rates in gloss, firmness (F4 value), skin texture, and the number of fine lines. The anti-aging emulsions in Examples 10-11 were superior to those in Examples 8-9 in terms of skin permeability. This indicates that a weight ratio of 1:(8-12) of edamame seed powder to enzymatic hydrolysate during the preparation of edamame seed extract is more effective in improving gloss, firmness, skin texture, and fine lines than a weight ratio of 1:(5-15).

[0186] Compared to Application Examples 7-11 and Application Examples 12-14, when the extraction pressure, extraction temperature, and extraction time used in the preparation of the *Edamame oleracea* seed extract in the anti-aging composition of Application Example 12 were outside the scope of this application (extraction pressure of 30-35 MPa, extraction temperature of 50-60°C, and extraction time of 1.5-3 h), and the preparation process of the *Edamame oleracea* seed extract in the anti-aging composition of Application Example 13 did not involve enzymatic hydrolysis, and the preparation process of the *Edamame oleracea* seed extract in the anti-aging composition of Application Example 14 used other types of enzymatic hydrolysates to obtain more *Edamame oleracea* seed polysaccharides and flavonoids, Application Examples 12-14 were less effective than Application Examples 7-11 in improving radiance, firmness, skin texture, and fine lines. Compared to Application Example 15, when other types of preparation methods were used for the *Edamame oleracea* seed extract, the resulting anti-aging compositions were less effective in improving radiance, firmness, skin texture, and fine lines.

[0187] Compared with application examples 1-3, which did not contain Camellia chrysantha extract and / or Edamame seed extract, and compared with application examples 11-12, which used other types of extracts to replace Camellia chrysantha extract or Edamame seed extract, the anti-aging lotions prepared showed lower improvement rates in gloss, firmness F4 value, skin texture score, and fine line count. This indicates that Camellia chrysantha extract and Edamame seed extract have a synergistic effect in improving gloss, firmness, skin texture, and fine lines.

[0188] Compared to application example 4, which does not contain μ-conospirin, and application example 5, which does not contain ascorbic acid peptides, the anti-aging lotion was less effective than application examples 1-7 in improving radiance, firmness, skin texture, and fine lines. Among them, Camellia chrysantha extract, in synergy with ascorbic acid peptides, better improved radiance, and the addition of μ-conospirin could better improve firmness; μ-conospirin, in synergy with ascorbic acid peptides, better improved the number of fine lines.

[0189] Compared with Application Examples 6 and 7, when the amount of anti-aging composition was kept constant, removing the Camellia chrysantha extract or Edamame seed extract alone resulted in anti-aging emulsions that were less effective in improving radiance, firmness, skin texture and fine lines than the anti-aging emulsion used in Application Example 7.

[0190] In comparative application examples 8-9, the mass ratio of Camellia chrysantha extract and Edamame seed extract was outside the scope of this application, and the anti-aging lotion was less effective in improving radiance, firmness, skin texture and fine lines.

[0191] Application Example 7 and the blank application example show the results of improved radiance and skin texture before and after 28 days of use. Figure 1 As shown.

[0192] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit the scope of protection of this application. Although this application has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the substance and scope of the technical solutions of this application.

Claims

1. An anti-aging composition, characterized in that, The anti-aging composition includes Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid peptide; The mass ratio of the Camellia chrysantha extract, Edamame seed extract, μ-conospirin, and ascorbic acid polypeptide is (1~2):(0.2~0.3):(0.1~0.2):(0.6~0.8). The preparation method of the grey soybean seed extract includes the following steps: 1) Add the gray soybean seed powder to the enzymatic hydrolysate for enzymatic hydrolysis, freeze-dry, and obtain the reactant; 2) The reactants were extracted by supercritical CO2 to obtain edamame seed extract.

2. The anti-aging composition according to claim 1, characterized in that, The conditions for supercritical CO2 extraction include: extraction pressure of 30~35 MPa, extraction temperature of 50~60℃, extraction time of 1.5~3 h, separation pressure of 5.5~6.5 MPa, separation temperature of 35~45℃, and CO2 flow rate of 15~30 L / h.

3. The anti-aging composition according to claim 1, characterized in that, In step 1), the weight ratio of gray soybean seed powder to enzymatic hydrolysate is 1:(5~15).

4. The anti-aging composition according to claim 3, characterized in that, In step 1), the weight ratio of gray soybean seed powder to enzymatic hydrolysate is 1:(8~12).

5. The anti-aging composition according to claim 1, characterized in that, In step 1), the enzymatic hydrolysate includes 2.5 million to 5 million U / L cellulase and 800,000 to 1.2 million U / L hemicellulase, and the pH of the enzymatic hydrolysate is 5.0 to 5.

5.

6. The anti-aging composition according to claim 1, characterized in that, In step 1), the enzymatic hydrolysis temperature is 45~55℃ and the enzymatic hydrolysis time is 1~2 hours.

7. The use of the anti-aging composition according to any one of claims 1 to 6 in the preparation of brightening, anti-aging, repairing or anti-sensitivity skin care products.

8. An anti-aging skincare product, characterized in that, The anti-aging skincare product includes a lotion; the lotion comprises the following components by weight percentage: Thickener 0.05-0.3%, humectant 0.5-3%, pH adjuster 0.01-0.3%, emulsifier 0.5-5%, preservative 0.5-3%, and anti-aging composition as described in any one of claims 1-6 1-5%.

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