A mesenchymal stem cell detection device

Abstract

This invention discloses a mesenchymal stem cell detection device, relating to the field of cell detection technology. It includes a detection unit comprising an operating table, a staining microscope mounted on top of the operating table, and a display screen mounted on top of the operating table. The operator places a culture dish on top of a fixing component and fixes it using a linkage component. A vibration component drives the linkage and fixing components, causing the culture dish to rotate sequentially to the bottom of the staining microscope for detection. The detection results are displayed on the display screen in real time. By using two staining microscopes, two culture dishes can be detected simultaneously and their data compared, ensuring the accuracy of the detection data. During cell staining, the rapid rotation of the vibration component promotes the mixing of the staining agent with the cells, reducing the staining mixing time.

Description

Technical Field

[0001] This invention relates to the field of cell detection technology, and more particularly to a mesenchymal stem cell detection device. Background Technology

[0002] Mesenchymal stem cells (MSCs) are a type of pluripotent stem cell, possessing all the common characteristics of stem cells, namely self-renewal and multi-lineage differentiation capabilities. After culturing MSCs under specific conditions, the cells need to be stained with a staining solution, and then the morphology of the MSCs is observed using a detection device to check for any defects or abnormalities.

[0003] In existing technologies, when culturing and testing mesenchymal stem cells under different growth environments, the detection results of mesenchymal stem cells under different environments by a single detection device have a certain time interval. This results in the culture time of cells that are detected later being longer than that of cells that are detected earlier, affecting the accuracy of the comparison results. In addition, existing detection devices are difficult to vibrate after staining to promote the rapid fusion of the staining agent with the cells, resulting in a longer staining cycle, increasing workload and time costs, and also affecting the reliability of experimental results. Summary of the Invention

[0004] In view of the problems existing in the above-mentioned mesenchymal stem cell detection devices, the present invention is proposed.

[0005] Therefore, the present invention provides a mesenchymal stem cell detection device, the purpose of which is to solve the problems of excessively long cell culture time and long staining cycle in cell detection technology due to long detection time intervals under different environments, difficulty in fixing culture dishes of different sizes and inability to effectively promote staining by vibration.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a mesenchymal stem cell detection device, comprising: a detection unit, including an operating table, a staining microscope disposed on the top of the operating table, and a display screen disposed on the top of the operating table, characterized in that:

[0007] The fixed mixing unit includes a vibration component disposed on the top of the operating table for mixing cell staining, a linkage component disposed inside the vibration component for fixing and driving the cell culture dish, and a fixing component disposed on the linkage component for maintaining the stability of the cell culture dish.

[0008] The vibration assembly includes a fixed housing mounted on the top of the operating table, a motor mounted on the bottom of the fixed housing, and a rotating rod mounted on the output end of the motor, with the other end of the rotating rod connected to the linkage assembly.

[0009] In a preferred embodiment of the mesenchymal stem cell detection device of the present invention, a ring plate is provided inside the fixed shell, and the ring plate is rotatably connected to the rotating rod, and a vibration block is provided on the top of the ring plate.

[0010] In a preferred embodiment of the mesenchymal stem cell detection device of the present invention, a top ring plate is provided on the outer diameter of the rotating rod, a movable part is provided at the bottom of the top ring plate, a roller is rotatably provided at the bottom of the movable part, and the roller is slidably connected to the vibrating block.

[0011] In a preferred embodiment of the mesenchymal stem cell detection device of the present invention, a buffer plate is provided on the outer diameter of the rotating rod, and the other end of the buffer plate is slidably connected to the moving part.

[0012] In a preferred embodiment of the mesenchymal stem cell detection device of the present invention, a reset spring is provided at the bottom of the buffer plate, and the other end of the reset spring is connected to the ring plate.

[0013] As a preferred embodiment of the mesenchymal stem cell detection device of the present invention, the linkage component includes a rotating disk disposed at the top of the rotating rod, a bottom shell disposed at the bottom of the rotating disk, a sliding plate disposed inside the bottom shell, a second reset spring disposed inside the sliding plate, and a locking block disposed at the other end of the second reset spring, wherein the locking block is slidably connected to the sliding plate.

[0014] As a preferred embodiment of the mesenchymal stem cell detection device of the present invention, a reset spring three is provided at the bottom of the bottom shell, a conical plate is provided at the other end of the reset spring three, and the conical plate cooperates with the locking block, and a T-shaped pressure plate is provided at the top of the conical plate, and the T-shaped pressure plate is slidably connected to the bottom shell.

[0015] In a preferred embodiment of the mesenchymal stem cell detection device of the present invention, a conical ring is provided on the bottom outer diameter of the T-shaped pressure plate, and the conical ring is slidably connected to the locking block.

[0016] As a preferred embodiment of the mesenchymal stem cell detection device of the present invention, the fixing component includes a rotating shaft rotatably disposed on the inner wall of the bottom shell, an L-shaped connecting rod disposed on the rotating shaft, and a fixing ring disposed at one end of the L-shaped connecting rod, wherein the other end of the L-shaped connecting rod is slidably connected to the T-shaped pressure plate.

[0017] In a preferred embodiment of the mesenchymal stem cell detection device of the present invention, the L-shaped connecting rod is slidably connected to the rotating disk.

[0018] The beneficial effects of this invention are as follows: The operator places the culture dish on top of the fixing component and fixes it through the linkage component. The vibration component drives the linkage component and the fixing component to rotate the culture dish sequentially to the bottom of the staining microscope for detection. The detection results are displayed on the screen in real time. By setting two staining microscopes, two culture dishes can be detected simultaneously and the data can be compared to ensure the accuracy of the detection data. During the staining process of cells, the vibration component rotates rapidly, which promotes the mixing of the staining agent and the cells, reduces the staining mixing time, and ensures stable fixation. This effectively solves the problems of long detection time intervals and long staining cycles in the prior art, and improves the detection efficiency and the reliability of experimental results. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the overall structure of the mesenchymal stem cell detection device of the present invention.

[0021] Figure 2 This is a schematic diagram of the detection unit structure of the mesenchymal stem cell detection device of the present invention.

[0022] Figure 3 This is a schematic diagram of the fixed mixing unit structure of the mesenchymal stem cell detection device of the present invention.

[0023] Figure 4 This is a schematic diagram of the internal structure of the fixed mixing unit of the mesenchymal stem cell detection device of the present invention.

[0024] Figure 5 The mesenchymal stem cell detection device of the present invention Figure 4 A magnified structural diagram at point A.

[0025] Figure 6 This is a schematic cross-sectional view of the fixed mixing unit of the mesenchymal stem cell detection device of the present invention.

[0026] Figure 7 This is a schematic cross-sectional view of the vibration component of the mesenchymal stem cell detection device of the present invention.

[0027] Figure 8 The mesenchymal stem cell detection device of the present invention Figure 7 A magnified structural diagram at point B.

[0028] Figure 9 This is a schematic cross-sectional view of the fixation component of the mesenchymal stem cell detection device of the present invention.

[0029] Figure 10 The mesenchymal stem cell detection device of the present invention Figure 9 A magnified structural diagram at point C.

[0030] Explanation of reference numerals in the attached drawings: 100, Detection unit; 101, Operating table; 102, Staining microscope; 103, Display screen; 200, Fixed mixing unit; 201, Vibration assembly; 2011, Fixed shell; 2012, Motor; 2013, Rotating rod; 2013-1, Return spring one; 2014, Ring plate; 2015, Vibrating block; 2016, Top ring plate; 2017, Moving part; 2018, Roller 2019. Wheel; 202. Buffer plate; 202. Linkage assembly; 2021. Rotary disk; 2022. Conical ring; 2023. Bottom shell; 2024. Slide plate; 2025. Second return spring; 2026. Clamping block; 2027. T-shaped pressure plate; 2028. Conical plate; 2029. Third return spring; 203. Fixing assembly; 2031. Rotating shaft; 2032. L-shaped connecting rod; 2033. Fixing ring. Detailed Implementation

[0031] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0032] Example 1, referring to Figures 1-3 The first embodiment of the present invention provides a mesenchymal stem cell detection device, which includes a detection unit 100 and a fixation and mixing unit 200.

[0033] The detection unit 100 includes an operating table 101, a staining microscope 102 disposed on the top of the operating table 101 for detecting through, and a display screen 103 disposed on the top of the operating table 101 for displaying data. The display screen 103 is electrically connected to the staining microscope 102 to display the data detected by the staining microscope 102 on the display screen 103.

[0034] The fixed mixing unit 200 includes a vibration component 201 disposed on the top of the operating table 101 for mixing cell staining, a linkage component 202 disposed inside the vibration component 201 for fixing and driving the cell culture dish, and a fixation component 203 disposed on the linkage component 202 for stabilizing the cell culture dish. The linkage component 202 and the fixation component 203 cooperate to quickly fix the cell culture dish. When detecting mesenchymal stem cells, the mesenchymal stem cell culture dish is placed on top of the fixation component 203, and the operator presses the culture dish into the linkage component 202, thereby causing the linkage component 202 to drive the fixation component 203. The fixing component 203 moves towards the culture dish and fixes it. After the linkage component 202 and the fixing component 203 have finished fixing the culture dish, the vibration component 201 starts to rotate, causing the linkage component 202 and the fixing component 203 to rotate together with the culture dish inside the linkage component 202 to the bottom of the staining microscope 102. The staining microscope 102 begins to detect and observe the cells in the culture dish, and the detection results are transmitted to the display screen 103 for display. By setting two staining microscopes 102, two culture dishes can be detected simultaneously, and the data of the two culture dishes can be compared to obtain accurate cell detection data.

[0035] After the staining microscope 102 completes the detection of the culture dish, the cells inside the culture dish need to be stained. The staining microscope 102 injects chromosomes into the culture dish to mix with the cells. After the chromosome injection is completed, the vibration component 201 starts to rotate rapidly, so that the vibration component 201, along with the linkage component 202, rotates the culture dish inside the fixation component 203, which accelerates the mixing of cells inside the culture dish, reduces the staining and mixing time, and the fixation by the linkage component 202 and the fixation component 203 can ensure that the cells inside the culture dish are fully mixed. After the cell staining and mixing is completed, the staining microscope 102 is used to detect the cells inside the culture dish again, so as to obtain the detection results quickly, ensure stable fixation, and effectively solve the problems of long detection time interval, difficulty in fixing culture dishes of different sizes, and long staining cycle in the prior art, thereby improving the detection efficiency and the reliability of experimental results.

[0036] During use, when testing mesenchymal stem cells, the culture dish containing the mesenchymal stem cells is placed on top of the fixation component 203. The operator then presses the culture dish into the linkage component 202, causing the linkage component 202 to drive the fixation component 203, which moves towards and fixes the culture dish. After the linkage component 202 and the fixation component 203 have fixed the culture dish, the vibration component 201 begins to rotate, causing the linkage component 202 and the fixation component 203, along with the culture dish inside the linkage component 202, to rotate one by one to the bottom of the staining microscope 102. The staining microscope 102 then begins to detect and observe the cells in the culture dish, and the detection results are displayed on the display screen 103. By setting up two staining microscopes 102, two culture dishes can be detected simultaneously, and the data from the two culture dishes can be compared to obtain accurate cell detection data.

[0037] After the staining microscope 102 completes the detection of the culture dish, the cells inside the culture dish need to be stained. The staining microscope 102 injects chromosomes into the culture dish to mix with the cells. After the chromosome injection is completed, the vibration component 201 starts to rotate rapidly, so that the vibration component 201, along with the linkage component 202, rotates the culture dish inside the fixation component 203, which accelerates the mixing of cells inside the culture dish, reduces the staining and mixing time, and the fixation by the linkage component 202 and the fixation component 203 can ensure that the cells inside the culture dish are fully mixed. After the cell staining and mixing is completed, the staining microscope 102 is used to detect the cells inside the culture dish again, so as to obtain the detection results quickly, ensure stable fixation, and effectively solve the problems of long detection time interval, difficulty in fixing culture dishes of different sizes, and long staining cycle in the prior art, thereby improving the detection efficiency and the reliability of experimental results.

[0038] Example 2, refer to Figures 1-7This is the second embodiment of the present invention, which differs from the first embodiment in that: the linkage component 202 includes a rotating disk 2021 disposed at the top of the rotating rod 2013, a bottom shell 2023 disposed at the bottom of the rotating disk 2021, a sliding plate 2024 disposed inside the bottom shell 2023, a second return spring 2025 disposed inside the sliding plate 2024, and a locking block 2026 disposed at the other end of the second return spring 2025, wherein the locking block 2026 is slidably connected to the sliding plate 2024, and a third return spring 2029 is disposed at the bottom of the bottom shell 2023, wherein a conical plate is disposed at the other end of the third return spring 2029. 2028, and the conical plate 2028 cooperates with the locking block 2026. The top of the conical plate 2028 is provided with a T-shaped pressure plate 2027, and the T-shaped pressure plate 2027 is slidably connected to the bottom shell 2023. The bottom outer diameter of the T-shaped pressure plate 2027 is provided with a conical ring 2022, and the conical ring 2022 is slidably connected to the locking block 2026. The fixing component 203 includes a rotating shaft 2031 rotatably disposed on the inner wall of the bottom shell 2023, an L-shaped connecting rod 2032 disposed on the rotating shaft 2031, and a fixing ring 2033 disposed at one end of the L-shaped connecting rod 2032. The other end of the L-shaped connecting rod 2032 is connected to the T-shaped pressure plate 2026. 027 is slidably connected, and L-shaped connecting rod 2032 is slidably connected to rotating disk 2021. When detecting mesenchymal cells, the operator places the culture dish containing cells inside rotating disk 2021 and simultaneously squeezes the culture dish downwards. This causes the culture dish to squeeze the T-shaped pressure plate 2027 inside rotating disk 2021, causing the T-shaped pressure plate 2027, along with the bottom conical plate 2028, to move downwards. During the movement of the T-shaped pressure plate 2027, it squeezes the locking blocks 2026 on both sides, causing the locking blocks 2026 to squeeze the second return spring 2025 and slide into the interior of the second return spring 2025. After the locking block 2026 presses and slides past the conical plate 2028, the locking block 2026 is released from pressure and directly locks onto the top of the conical plate 2028. When the T-shaped pressure plate 2027 moves into the bottom shell 2023, the T-shaped pressure plate 2027 also presses against the rotating shaft 2031, causing the rotating shaft 2031, along with the L-shaped connecting rod 2032 and the fixing ring 2033, to rotate and adjust inside the bottom shell 2023. This causes the fixing ring 2033 to lock onto the outer diameter of the culture dish at the top, thus completing the rapid fixation of the culture dish. Then, the staining microscope 102 is used to begin the detection of cells inside the culture dish.

[0039] After the cell detection is completed, simply squeeze the culture dish downwards again to compress the T-shaped pressure plate 2027. This will cause the locking block 2026 on the outer diameter of the T-shaped pressure plate 2027 to be squeezed again and slide to the top of the conical ring 2022. Under the guidance of the conical ring 2022 and the reaction force of the return spring 2029, the T-shaped pressure plate 2027 will be released and move upwards. At the same time, the rotating shaft 2031 will also be released from compression. The L-shaped connecting rod 2032 and the fixing ring 2033 will unfold from both sides of the culture dish, allowing the culture dish to be removed.

[0040] During use, when testing mesenchymal cells, the operator places the culture dish containing the cells inside the rotating disk 2021 and simultaneously squeezes the culture dish downwards. This causes the culture dish to press against the T-shaped pressure plate 2027 inside the rotating disk 2021, causing the T-shaped pressure plate 2027, along with the bottom conical plate 2028, to move downwards. During this movement, the T-shaped pressure plate 2027 squeezes the locking blocks 2026 on both sides, causing the locking blocks 2026 to press against the second return spring 2025 and slide inside it. The locking blocks 2026 then slide past... After the conical plate 2028 is removed, the locking block 2026 is released from compression and directly locked onto the top of the conical plate 2028. When the T-shaped pressure plate 2027 moves into the bottom shell 2023, the T-shaped pressure plate 2027 also presses against the rotating shaft 2031, causing the rotating shaft 2031, along with the L-shaped connecting rod 2032 and the fixing ring 2033, to rotate and adjust inside the bottom shell 2023. This causes the fixing ring 2033 to lock onto the outer diameter of the culture dish at the top, thus completing the rapid fixation of the culture dish. Then, the cells inside the culture dish can be detected by the staining microscope 102.

[0041] After cell detection is completed, simply press the culture dish downwards again to compress the T-shaped pressure plate 2027. This will cause the locking block 2026 on the outer diameter of the T-shaped pressure plate 2027 to be compressed again and slide to the top of the conical ring 2022. Under the guidance of the conical ring 2022 and the reaction force of the return spring 2029, the T-shaped pressure plate 2027 will be released and move upwards. At the same time, the rotating shaft 2031 will also be released from compression. The L-shaped connecting rod 2032 and the fixing ring 2033 will unfold from both sides of the culture dish, allowing the culture dish to be removed. This reduces the fixation time of the culture dish and enhances the efficiency of cell detection.

[0042] The remaining structure is the same as that in Example 1.

[0043] Example 3, referring to Figures 1-10This is the third embodiment of the present invention, which differs from the second embodiment in that: the vibration assembly 201 includes a fixed shell 2011 disposed on the top of the operating table 101, a motor 2012 disposed on the bottom of the fixed shell 2011, and a rotating rod 2013 disposed on the output end of the motor 2012, with the other end of the rotating rod 2013 connected to the linkage assembly 202. An annular plate 2014 is disposed inside the fixed shell 2011, and the annular plate 2014 is rotatably connected to the rotating rod 2013. A vibration block 2015 is disposed on the top of the annular plate 2014, a top annular plate 2016 is disposed on the outer diameter of the rotating rod 2013, and a moving part 2017 is disposed at the bottom of the top annular plate 2016. The bottom of the moving part 2017 rotates... The rotating rod 2013 is equipped with a roller 2018, which is slidably connected to the vibrating block 2015. A buffer plate 2019 is installed on the outer diameter of the rotating rod 2013, and the other end of the buffer plate 2019 is slidably connected to the moving part 2017. A return spring 2013-1 is installed at the bottom of the buffer plate 2019, and the other end of the return spring 2013-1 is connected to the ring plate 2014. After the fixing component 203 fixes the culture dish under the drive of the linkage component 202, the motor 2012 starts working, causing the rotating rod 2013 to rotate with the top rotating disk 2021 and the culture dish. With the cooperation of the staining microscope 102, multiple culture dishes are detected and observed. During the detection, the staining microscope 102 is used to detect and observe the culture dishes. The staining microscope 102 adds a detection dye to the inside of the culture dish. After the staining microscope 102 adds the dye, the motor 2012 increases its rotation speed, causing the rotating disk 2021 at the top of the rotating rod 2013 and the culture dish to rotate rapidly. At the same time, the rotating rod 2013 also causes the top ring plate 2016 and the moving part 2017 and roller 2018 at the bottom of the top ring plate 2016 to start rotating. As the roller 2018 rotates, it rotates along the vibrating block 2015 on the ring plate 2014. Under the action of the vibrating block 2015, the roller 2018 and the moving part 2017 continuously reciprocate up and down during the rotation, thereby causing the top ring plate 2016 to move along the top linkage assembly. The staining device 202 and the fixing component 203 vibrate up and down together with the culture dish inside the fixing component 203, which accelerates the mixing of the staining agent and cells inside the culture dish and reduces the mixing time of the staining agent and cells. At the same time, the vibration response of the culture dish inside the rotating disk 2021 is reduced by the cooperation of the buffer plate 2019 on the outer diameter of the rotating rod 2013 and the return spring 2013-1. With the cooperation of the fixing component 203, it is ensured that the staining agent and cells are fully mixed and that the stability of the culture dish is maintained during the rotation and vibration mixing process. After the staining agent and cells are mixed, the cells are still detected by the staining microscope 102, which improves the detection efficiency and enhances the detection accuracy.

[0044] During use, after the fixing component 203 fixes the culture dish under the drive of the linkage component 202, the motor 2012 starts working, causing the rotating rod 2013 to rotate along with the top rotating disk 2021 and the culture dish. With the cooperation of the staining microscope 102, multiple culture dishes are inspected and observed. During inspection, the staining microscope 102 adds the detection stain to the inside of the culture dish. After the staining microscope 102 adds the stain to the inside of the culture dish, the motor 2012 increases the rotation speed, causing the rotating disk 2021 at the top of the rotating rod 2013 and the culture dish to rotate rapidly. At the same time, the rotating rod 2013 also causes the top ring plate 2016 and the moving part 2017 and roller 2018 at the bottom of the top ring plate 2016 to start rotating. When the roller 2018 rotates, it rotates along the vibrating block 2015 on the ring plate 2014. Under the action of the vibrating block 2015, the roller 2018 and the moving part 2017 rotate. During rotation, the device continuously moves up and down, causing the top ring plate 2016, along with the top linkage component 202 and fixing component 203, to vibrate up and down together with the culture dish inside the fixing component 203. This accelerates the mixing of the staining agent and cells inside the culture dish and reduces the mixing time. Simultaneously, the buffer plate 2019 on the outer diameter of the rotating rod 2013 and the return spring 2013-1 reduce the vibration response of the culture dish inside the rotating disk 2021. With the cooperation of the fixing component 203, it ensures that the staining agent and cells are fully mixed and that the culture dish remains stable during the rotation and vibration mixing process. After the staining agent and cells are mixed, the cells are further examined by the staining microscope 102, improving the efficiency and accuracy of the detection. This effectively solves the problems of long detection time intervals and long staining cycles in existing technologies, improving detection efficiency and the reliability of experimental results.

[0045] The remaining structure is the same as that in Example 2.

[0046] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A mesenchymal stem cell detection device, comprising: The detection unit (100) includes an operating table (101), a staining microscope (102) disposed on top of the operating table (101), and a display screen (103) disposed on top of the operating table (101), characterized in that: The fixed mixing unit (200) includes a vibration component (201) disposed on the top of the operating table (101) for mixing cell staining, a linkage component (202) disposed inside the vibration component (201) for fixing and driving the cell culture dish, and a fixing component (203) disposed on the linkage component (202) for keeping the cell culture dish stable. The vibration assembly (201) includes a fixed housing (2011) disposed on the top of the operating table (101), a motor (2012) disposed on the bottom of the fixed housing (2011), and a rotating rod (2013) disposed on the output end of the motor (2012), and the other end of the rotating rod (2013) is connected to the linkage assembly (202); The linkage assembly (202) includes a rotating disk (2021) disposed at the top of the rotating rod (2013), a bottom shell (2023) disposed at the bottom of the rotating disk (2021), a sliding plate (2024) disposed inside the bottom shell (2023), a second return spring (2025) disposed inside the sliding plate (2024), and a locking block (2026) disposed at the other end of the second return spring (2025), and the locking block (2026) is slidably connected to the sliding plate (2024); The bottom of the bottom shell (2023) is provided with a return spring three (2029), and the other end of the return spring three (2029) is provided with a conical plate (2028), and the conical plate (2028) cooperates with the locking block (2026). The top of the conical plate (2028) is provided with a T-shaped pressure plate (2027), and the T-shaped pressure plate (2027) is slidably connected to the bottom shell (2023). The bottom outer diameter of the T-shaped pressure plate (2027) is provided with a conical ring (2022), and the conical ring (2022) is slidably connected to the locking block (2026); The fixing component (203) includes a rotating shaft (2031) rotatably mounted on the inner wall of the bottom shell (2023), an L-shaped connecting rod (2032) mounted on the rotating shaft (2031), and a fixing ring (2033) mounted on one end of the L-shaped connecting rod (2032), and the other end of the L-shaped connecting rod (2032) is slidably connected to the T-shaped pressure plate (2027).

2. The mesenchymal stem cell detection device according to claim 1, characterized in that: The fixed shell (2011) is provided with an annular plate (2014) inside, and the annular plate (2014) is rotatably connected to the rotating rod (2013). A vibrating block (2015) is provided on the top of the annular plate (2014).

3. The mesenchymal stem cell detection device according to claim 2, characterized in that: The outer diameter of the rotating rod (2013) is provided with a top ring plate (2016), the bottom of the top ring plate (2016) is provided with a movable part (2017), the bottom of the movable part (2017) is rotatably provided with a roller (2018), and the roller (2018) is slidably connected to the vibrating block (2015).

4. The mesenchymal stem cell detection device according to claim 3, characterized in that: The outer diameter of the rotating rod (2013) is provided with a buffer plate (2019), and the other end of the buffer plate (2019) is slidably connected to the moving part (2017).

5. The mesenchymal stem cell detection device according to claim 4, characterized in that: The bottom of the buffer plate (2019) is provided with a return spring (2013-1), and the other end of the return spring (2013-1) is connected to the ring plate (2014).

6. The mesenchymal stem cell detection device according to claim 5, characterized in that: The L-shaped connecting rod (2032) is slidably connected to the rotating disk (2021).

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