An adjustable fluorescence microscope

Abstract

The application relates to the technical field of microscopes, and discloses an adjustable fluorescence microscope which comprises a base, a mirror arm arranged on the top of the base, and a sample platform arranged on one side of the mirror arm; the top surface of the base is provided with a lifting device; and one end of the sample platform is movably connected with the lifting device. The fluorescence microscope controls the sample platform to move downwards through the lifting device; a first filter automatically turns over around a fixed shaft to the outside of the sample platform under the action of gravity, thereby automatically providing a horizontal operation space for the sample platform; after sample replacement is completed, the sample platform only needs to be lifted to a preset observation position; when the sample is replaced, the sample platform only needs to be controlled to move, manual operation of the filter is not required, so that the sample can be replaced by the staff in a high-frequency sample replacement scene, the human time cost for sample detection is shortened, and the overall working efficiency and operation convenience of the microscopic observation system are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microscopy, in particular to an adjustable fluorescence microscope. BACKGROUND

[0002] A fluorescence microscope is a special optical microscope that uses fluorescence phenomenon to observe and study the structure and characteristics of biological samples or materials through the detection of fluorescence signals, providing high sensitivity and high specificity imaging results, and providing strong support for scientific research and practical applications, and is widely used in the fields of biomedical science, material science, environmental science, etc.

[0003] A microscope light shielding structure disclosed in Chinese Patent No. CN110320655A includes a fixed structure, a light filtering structure, and an adjusting structure for adjusting the position of the light filtering structure. The fixed structure is arranged on a receiving plate between the objective lens and the eyepiece of the microscope. The fixed structure is combined with the microscope, the fixed structure is combined with the light filtering structure, and the light filtering structure is combined with the adjusting structure. During use, the motor structure drives the semicircular gear to rotate, thereby driving the light filter to rotate, allowing the light filter to open a certain distance to the outside of the microscope, thereby facilitating the operator to adjust the slide and other operations.

[0004] The existing technology has the problem of redundant operation. Specifically, when the operator replaces the sample, the operator first needs to control the sample platform to perform longitudinal lowering to create a vertical operation space (i.e., lower the sample platform), and then needs to drive the light filter to open laterally to the outside of the microscope to expand the horizontal operation space. After completing the sample replacement, the operator needs to perform reverse resetting operation to control the sample platform to vertically lift to the preset observation position, and then move the light filter assembly back to the reset position. This repeated manual operation of the light filter not only reduces the observation efficiency in the high-frequency sample replacement scenario, but also forces the operator to perform two independent operations (i.e., two operations to drive the light filter) during a single sample replacement process. When facing the demand for batch sample detection, the cumulative human time cost will be multiplied, which restricts the overall work efficiency of the microscope observation system. SUMMARY

[0005] To achieve the above purpose, the present application provides an adjustable fluorescence microscope, which comprises a base, a mirror arm arranged on the top of the base, and a sample platform arranged on one side of the mirror arm. The top surface of the base is provided with a lifting device, and one end of the sample platform is movably connected with the lifting device.

[0006] One side of the mirror arm is provided with a fixed shaft, the fixed shaft penetrates the two sides of the object platform, a first filter is rotatably arranged on the fixed shaft, and limiting columns for limiting the first filter on the two sides of the object platform are arranged on the two sides of the object platform; when the lifting device controls the upward movement of the object platform, the first filter is turned upward around the fixed shaft under the blocking of the limiting columns to form a light filtering area, and when the object platform moves downward, the first filter is automatically turned downward around the fixed shaft under the action of gravity.

[0007] Further, a through groove is formed between the limiting column and the object platform, and the first filter is arranged in the through groove.

[0008] Further, a fixing device is fixedly arranged at the end of the fixed shaft away from the mirror arm, and the fixing device is used for limiting and fixing the first filter.

[0009] Further, the two sides of the bottom of the object platform are provided with clamping grooves corresponding to the fixed shaft.

[0010] Further, one side of the bottom of the object platform is provided with a control knob, the lifting device comprises a servo motor arranged on the base, one side of the servo motor is provided with a steering gear set, one end of the steering gear set is rotatably provided with a transmission screw rod, the transmission screw rod is rotatably connected with the object platform, the top end of the transmission screw rod is provided with a screw rod top plate, and the screw rod top plate is fixedly connected with the mirror arm.

[0011] Further, the fixing device comprises a positioning partition plate fixedly arranged at one end of the fixed shaft, a ratchet clamping column movably arranged in the positioning partition plate, a pawl fixedly arranged at one end of the ratchet clamping column, a torsion spring arranged on the outer side of the ratchet clamping column, a one-way ratchet rotatably arranged on the end of the fixed shaft away from the mirror arm and corresponding to the pawl, a limiting clamping column fixedly arranged on one side of the one-way ratchet, the limiting clamping column is used for abutting against the outer side of the first filter to limit and fix the first filter, and the limiting clamping column and the limiting column are staggered with each other.

[0012] Further, the limiting clamping column comprises a connecting block fixedly connected with the one-way ratchet, a limiting rod is fixedly arranged at the end of the connecting block away from the one-way ratchet, and the limiting rod extends to the outer side of the first filter.

[0013] Further, one side of the mirror arm is fixedly provided with a positioning device, a second filter is movably arranged on one side of the positioning device, the bottom of the second filter is in contact with the object platform, and when the object platform moves upward, the second filter moves upward together with the object platform and surrounds the first filter to form a light filtering area.

[0014] Further, the positioning device comprises a fixed plate fixedly arranged on one side of the mirror arm, a side edge positioning plate is fixedly arranged on the top of the fixed plate, support bottom columns are arranged on the top of the fixed plate and located on both sides of the side edge positioning plate, and baffle assemblies are fixedly arranged on both sides of the side edge positioning plate.

[0015] Further, the second filter comprises a filter main plate movably arranged on one side of the side edge positioning plate, filter side plates are fixedly arranged on both sides of the filter main plate away from the side edge positioning plate, a moving groove is formed in the filter main plate close to the side edge positioning plate, the moving groove is matched with the side edge positioning plate, and a limiting baffle is fixedly arranged on the filter main plate close to the side edge positioning plate, the limiting baffle, the support bottom columns and the baffle assemblies correspond to each other.

[0016] Compared with the prior art, the present application provides a fluorescence microscope, which has the following beneficial effects:

[0017] 1. The lifting device controls the downward movement of the object platform, the first filter automatically flips around the fixed shaft to the outside of the object platform under the action of gravity, thereby automatically providing a horizontal operation space for the object platform, and after the sample replacement is completed, the object platform only needs to be lifted to the preset observation position; the scheme disclosed in the present application only needs to control the movement of the object platform when the sample is replaced, without the need for repeated manual operation of the filter, thereby facilitating the replacement of the sample by the staff in the high-frequency sample replacement scenario, and further shortening the labor time cost of sample detection and improving the overall working efficiency and operation convenience of the microscopic observation system.

[0018] 2. When the object platform is finely adjusted (for example, when the focal length is adjusted, the fine adjustment is downward fine adjustment), the first filter can be fixed by the fixing device to avoid downward flipping of the first filter under the action of gravity, and the first filter remains stationary when the downward fine adjustment is performed, thereby avoiding the influence on the filtering effect of the filtering area. When the first filter and the second filter cooperate to form a three-sided filtering area, the first filter can be fixed by the fixing device to avoid the generation of a gap between the first filter and the second filter, thereby further guaranteeing the filtering effect of the filtering area. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0020] Figure 1 It is a schematic diagram of the overall structure of the adjustable fluorescence microscope.

[0021] Figure 2 This is a schematic diagram of the standby state structure of an adjustable fluorescence microscope according to the present invention;

[0022] Figure 3 This is a schematic diagram of the back structure of an adjustable fluorescence microscope according to the present invention;

[0023] Figure 4 This is a schematic diagram of the second filter structure of the present invention;

[0024] Figure 5 This is a schematic diagram of the lifting device structure of the present invention;

[0025] Figure 6 This is a schematic diagram of the cargo platform structure of the present invention;

[0026] Figure 7 This is a schematic diagram of the first filter structure of the present invention;

[0027] Figure 8 For the present invention Figure 7 Enlarged schematic diagram of the structure at point A in the middle;

[0028] Figure 9 For the present invention Figure 8 Enlarged schematic diagram of the structure at point B;

[0029] Figure 10 For the present invention Figure 7 Enlarged schematic diagram of the structure at point C.

[0030] Figure label:

[0031] 11, base; 12, light source device; 13, focusing knob; 14, mirror arm; 15, wide-angle eyepiece; 16, charge-coupled device; 17, achromatic objective; 22, object platform; 23, specimen holder; 231, fixing clamp; 232, specimen card slot; 233, rotating clamp; 234, rotating handle; 25, lifting device; 251, servo motor; 252, steering gear set; 253, transmission screw; 254, screw top plate; 255, control button; 26, card slot; 27, limiting column; 28, control knob; 31, fixed shaft; 32, lower filter plate; 33, first filter; 331, filter main body; 332, support column; 333, extraction frustum; 334, connecting column; 335, insertion frustum; 336, arc-shaped clamping strip; 34, limiting block; 35, connecting device; 351, connecting ring; 352, butt block; 353, elastic column; 354, triangular clamping column; 36, connecting fixed plate; 37, fixing device; 371, positioning partition plate; 372, ratchet clamping column; 373, torsion spring; 374, pawl; 375, protection partition plate; 376, one-way ratchet; 377, limiting clamping column; 41, second filter; 411, filter main plate; 412, filter side plate; 413, moving slot; 414, limiting baffle; 42, positioning device; 421, fixed plate; 422, side positioning plate; 423, support bottom column; 424, baffle assembly. DETAILED DESCRIPTION

[0032] To make the objects, technical solutions, and advantages of the embodiments of the present application clearer, the following will briefly describe the technical solutions in the embodiments of the present application with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are some but not all of the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0033] The present application will be further described in connection with the embodiments.

[0034] Embodiment 1

[0035] Reference Figure 1 For the first embodiment of the present application, an adjustable fluorescence microscope is provided, which comprises a base 11, a mirror arm 14 fixedly arranged on the top of the base 11, and an object platform 22 arranged on one side of the mirror arm 14. One side of the mirror arm 14 is provided with a fixed shaft 31, the fixed shaft 31 penetrates through both sides of the object platform 22, and a first filter 33 is rotatably arranged on the fixed shaft 31. The top surface of the base 11 is provided with a lifting device 25, and the lifting device 25 is movably connected to the side of the object platform 22 close to the mirror arm 14. The lifting device 25 is used to drive the object platform 22 to move up and down, so as to facilitate the staff to replace or observe the sample.

[0036] Referring to Figure 6 Both sides of the object platform 22 are fixed with limiting columns 27 which limit the first filter 33 on both sides of the object platform 22, and a through slot is formed between the limiting columns 27 and the object platform 22, and the first filter 33 is arranged in the through slot (as shown in Figure 1 The purpose of forming the through slot is to facilitate the movement of the first filter 33 in the movement direction of the object platform 22, and to avoid the direct contact between the first filter 33 and the object platform 22, so as to prevent the abrasion of the first filter 33.

[0037] Continuously referring to Figure 6 The bottom of the object platform 22 is provided with clamping grooves 26 corresponding to the fixed shafts 31, and the clamping grooves 26 gradually approach and coincide with the fixed shafts 31 during the downward movement of the object platform 22, and at this time, the object platform 22 is in the maximum range of downward movement, so as to prevent the downwardly turned first filter 33 from blocking the light source device 12, and facilitate the adjustment or replacement of the light source device 12 according to the actual needs.

[0038] Referring to Figure 7 The fixed shafts 31 are fixedly provided with a fixing device 37 away from one end of the mirror arm 14, and the fixing device 37 is used to keep the first filter 33 stationary when the object platform 22 is finely adjusted downward, that is, the first filter 33 is in a vertical state, so as to guarantee the filtering effect (see the following description).

[0039] One side of the mirror arm 14 is fixedly provided with a positioning device 42 (as shown in Figure 5 One side of the positioning device 42 is movably provided with a second filter 41, and the bottom of the second filter 41 is in contact with the object platform 22. When the object platform 22 moves upward, the second filter 41 moves upward with the object platform 22, and surrounds the first filter 33 to form a filtering area.

[0040] When the lifting device 25 controls the upward movement of the object platform 22, the second filter 41 is driven to move upward, and the outer side of the first filter 33 is extruded by the limiting columns 27 on both sides of the object platform 22, so that the first filter 33 is turned upward around the fixed shaft 31 and surrounds the second filter 41 to filter light; when the object platform 22 moves downward, the first filter 33 is automatically turned downward around the fixed shaft 31 under the action of gravity, and when the object platform 22 is finely adjusted (for example, the fine adjustment is downward adjustment when the focal length is adjusted), the first filter 33 can be fixed by the fixing device 37 to avoid the downward turning of the first filter 33 under the action of gravity. When the object platform 22 is finely adjusted downward, the first filter 33 remains stationary to avoid affecting the filtering effect of the filtering area. When the first filter 33 and the second filter 41 cooperate to form a three-sided filtering area, the first filter 33 is fixed by the fixing device 37, which can also avoid the gap between the first filter 33 and the second filter 41, and further guarantee the filtering effect of the filtering area.

[0041] Compared with the prior art, in the process of replacing the sample, the carrier platform 22 is controlled to move downward, the first filter 33 is automatically flipped around the fixed shaft 31 to the outside of the carrier platform 22 under the action of gravity (as shown in Figure 2 After the sample is replaced, the carrier platform 22 only needs to be lifted to the preset observation position, that is, in the process of upward movement of the carrier platform 22, the second filter 41 is automatically formed with the first filter 33 to form a filtering area on three sides of the sample (as shown in Figure 1 The second filter 41 further effectively blocks external light and stray light inside the microscope, improves the signal-to-noise ratio of the image, and makes the observation more accurate.

[0042] When the sample is replaced, the carrier platform 22 only needs to be controlled to move, without the need for repeated manual operation of the filter, so as to facilitate the replacement of the sample by the staff in the high-frequency sample replacement scenario, thereby shortening the labor time cost of sample detection and improving the overall working efficiency and operation convenience of the microscopic observation system. The specific principle is described as follows:

[0043] Specifically, the lifting device 25 controls the carrier platform 22 to move up and down freely, and in the process of downward movement of the carrier platform 22, since the fixed shaft 31 is fixedly installed on one side of the mirror arm 14 and the first filter 33 is rotationally connected to the surface of the fixed shaft 31, the first filter 33 will rotate along the surface of the fixed shaft 31 under the action of gravity and flip downward along the moving direction of the carrier platform 22 to the outside of the carrier platform 22, until the carrier platform 22 is located at the lowest position set on the lifting device 25, at which time the included angle between the first filter 33 and the vertical plane is between 5°-10° (as shown in Figure 2 Then the staff places the carrier glass on the carrier platform 22 (the sample is placed on the carrier glass) and fixes it, and after the carrier glass is placed, the lifting device 25 is started again to control the carrier platform 22 to move upward, at which time the carrier platform 22 moves upward along the lifting device 25, and the limiting column 27 on the carrier platform 22 extrudes the outside of the first filter 33, so that the first filter 33 flips upward along the fixed shaft 31, until the inside of the first filter 33 is attached to the outside of the second filter 41, and the second filter 41 can also function as a horizontal support for the first filter 33, preventing the first filter 33 from being in contact with the carrier platform 22 and causing friction, which affects the filtering effect of the first filter 33.

[0044] As the platform 22 continues to move upward, it first contacts the bottom of the second filter 41, causing the second filter 41 to move upward along with the platform 22 until the platform 22 reaches the set observation position. At this time, the first filter 33 and the second filter 41 block and filter the light around the slide on the platform 22, thereby effectively blocking external light and stray light inside the microscope, reducing interference from non-specific fluorescence, improving the signal-to-noise ratio of the image, and making the observation more accurate.

[0045] Example 2

[0046] Reference Figure 8 and Figure 9 As shown in the second embodiment of the present invention, an adjustable fluorescence microscope is provided. The first filter 33 includes a filter body 331. A support column 332 is fixedly provided at the bottom of the filter body 331. A connecting column 334 is fixedly provided at the bottom of the support column 332. An extraction cone 333 is movably provided on the outer side of the connecting column 334. An insertion cone 335 is fixedly provided at the top of the connecting column 334. An arc-shaped retaining strip 336 located on one side of the support column 332 is provided at the bottom of the filter body 331. The outer side of the fixed shaft 31 is movably provided. A limiting block 34 is provided, and the arc surface of the arc-shaped locking strip 336 matches the protrusion on the limiting block 34. The limiting block 34 prevents the filter body 331 from continuing to move downward and insert into the connecting device 35 due to its own weight when the filter body 331 forms a filtering area with the filter side plate 412. This would cause the filter body 331 to loosen from the connecting device 35, and then, during the downward movement of the carrying platform 22, when the filter body 331 follows it downward and flips, it would loosen from the connecting device 35 and fall, causing the filter body 331 to fall and be damaged.

[0047] A connecting device 35 is movably provided on the outer side of the fixed shaft 31, located on one side of the limiting block 34, and a connecting plate 36 is fixedly provided on the outer side of the fixed shaft 31. Figure 4 As shown), the connecting plate 36 is fixedly connected to the mirror arm 14, and the lower filter plate 32 is fixedly provided on the outside of the fixed shaft 31. The lower filter plate 32 is used to block and filter the light below the platform 22, further improving the filtering effect during the detection process.

[0048] The connecting device 35 comprises a connecting ring 351 which is rotatably sleeved on the surface of the fixed shaft 31, the top of the connecting ring 351 is fixedly provided with a butt joint block 352, the butt joint block 352 is internally provided with a butt joint groove, the both sides of the butt joint groove are fixedly provided with elastic columns 353, one end of each of the elastic columns 353 is fixedly provided with a triangular clamping column 354, the inclined surface of the triangular clamping column 354 faces upward, and the filter main body 331 can be quickly disassembled from the fixed shaft 31 only when the inclined surface of the triangular clamping column 354 faces upward, so that the staff can replace the filter main body 331 with different colors according to the types of samples and light sources, and specific principles are described below in the embodiment.

[0049] Referring to Figure 7 and Figure 10 The fixing device 37 comprises a positioning partition plate 371 fixedly arranged at the end of the fixed shaft 31 away from the mirror arm 14, the positioning partition plate 371 is rotatably provided with a ratchet clamping column 372 in the inside, one end of the ratchet clamping column 372 is fixedly provided with a pawl 374, the outer side of the ratchet clamping column 372 is provided with a torsion spring 373, the torsion spring 373 is used to make the pawl 374 always engage with a one-way ratchet 376, the end of the fixed shaft 31 away from the mirror arm 14 is rotatably sleeved with the one-way ratchet 376 corresponding to the pawl 374, the one-way ratchet 376 can be connected with the fixed shaft 31 through a bearing, and the one-way ratchet 376 is fixedly provided with a limiting clamping column 377 on one side, which is used to abut against the outer side of the first filter 33 to limit and fix the first filter 33.

[0050] The limiting clamping column 377 comprises a connecting block fixedly connected with the one-way ratchet 376, and the end of the connecting block away from the one-way ratchet 376 is fixedly provided with a limiting rod which extends to the outer side of the first filter 33.

[0051] In order to protect the filter main body 331, a protection partition plate 375 is arranged between the one-way ratchet 376 and the filter main body 331, the protection partition plate 375 is fixedly sleeved on the surface of the fixed shaft 31 and does not interfere with the movement of the limiting rod.

[0052] Meanwhile, the limiting clamping column 377 and the limiting column 27 are staggered with each other, so that the limiting clamping column 377 and the limiting column 27 do not collide with each other when the object platform 22 moves downward to the lowest position, and the object platform 22 can normally move downward.

[0053] Specifically, before the fluorescence microscope is used, the light source device 12 corresponding to the sample to be detected is selected, and then the filter main body 331 and the second filter 41 of the type suitable for the light source device 12 are selected, then the limiting block 34 is moved along the outer side of the fixed shaft 31 to the side of the connecting ring 351, so that the limiting block 34 and the connecting ring 351 are embedded together, at this time, the limiting block 34 and the connecting ring 351 which are folded together are held (as shown in FIG. 9), and then the filter main body 331 is disassembled from the fixed shaft 31. Figure 8As shown), then the insertion cone 335 at the bottom of the filter body 331 is aligned with the docking groove of the docking block 352 on the connecting ring 351 until the insertion cone 335 is completely inserted into the docking groove inside the docking block 352, at which time the elastic columns 353 inside the docking groove on both sides will limit the insertion cone 335 (as shown Figure 9 As shown), at which time the arc-shaped clamping strip 336 will be attached together with the top of the limiting block 34, thereby limiting the depth of the insertion cone 335 inserted into the docking block 352, and the other set of insertion cones 335 are inserted together with the connecting device 35, thereby detachably fixing the filter body 331 to the outside of the fixed shaft 31.

[0054] When it is necessary to replace the filter body 331, first separate the limiting block 34 from the connecting ring 351, and move the limiting block 34 out of the range of the arc-shaped clamping strip 336 along the surface of the fixed shaft 31, and then the limiting block 34 naturally flips down along the fixed shaft 31 under the action of gravity, then hold the connecting ring 351, and continue to move the filter body 331 to the connecting ring 351 (at this time, the limiting block 34 is separated from the arc-shaped clamping strip 336, that is, the limiting block 34 is away from below the arc-shaped clamping strip 336, and the filter body 331 can continue to be pressed down without the interference of the limiting block 34), then the insertion cone 335 continues to penetrate into the docking groove, and the extraction cone 333 moves together with the insertion cone 335, until the insertion cone 335 moves to the bottom of the docking groove, at which time continue to move the filter body 331 downward, so that the extraction cone 333 continues to move downward along the connecting column 334, until the bottom of the extraction cone 333 is attached together with the top of the insertion cone 335, and at the same time, the triangular clamping columns 354 at one end of the elastic columns 353 are located on both sides of the extraction cone 333, then pull out the filter body 331 outwardly, since the extraction cone 333 is not completely inserted into the docking groove, and then the extraction cone 333 will drive the insertion cone 335 to pass through the two sets of triangular clamping columns 354, thereby taking out the insertion cone 335 from the docking block 352.

[0055] At the same time, when the extraction cone 333 leaves the clamping of the triangular clamping column 354, the spring inside the extraction cone 333 will automatically reset the extraction cone 333, so as to facilitate the staff to reinsert the filter body 331 into the docking block 352 next time, or to replace the type of filter body 331 according to the type of light source, thereby increasing the application range of the device.

[0056] When the object platform 22 is fine-tuned up and down, the filter body 331 will automatically turn down around the fixed shaft 31 under the action of gravity when the object platform 22 moves down, so that a gap is generated between the filter body 331 and the second filter 41, at this time the staff holds the outside of the limiting clamping column 377, and rotates the limiting clamping column 377 counterclockwise around the fixed shaft 31, and then the limiting clamping column 377 drives the one-way ratchet wheel 376 to rotate until the limiting clamping column 377 is attached to one side of the filter body 331, at this time the limiting clamping column 377 is released, and the filter body 331 is blocked in the counterclockwise direction by the limiting clamping column 377. Under the restriction of the pawl 374, the one-way ratchet wheel 376 can only rotate counterclockwise, and cannot rotate clockwise.

[0057] After the fine-tuning of the object platform 22 is completed, the ratchet wheel clamping column 372 is rotated at this time, so that the pawl 374 at one end of the ratchet wheel clamping column 372 is separated from the one-way ratchet wheel 376, and then the limiting clamping column 377 is rotated clockwise to reset, and the limiting clamping column 377 is separated from the filter body 331, and the restriction on the downward turning of the filter body 331 is released (as shown in Figure 7 ). When the object platform 22 is fine-tuned down, the first filter 33 is kept stationary, that is, the first filter 33 is in a vertical state, which guarantees the filtering effect.

[0058] Then the ratchet wheel clamping column 372 is released, and then the torsion spring 373 outside the ratchet wheel clamping column 372 will reset it and make the pawl 374 re-clamp the one-way ratchet wheel 376 to limit and fix it.

[0059] Embodiment 3

[0060] With reference to Figures 3-5 For the third embodiment of the present application, an adjustable fluorescence microscope is provided, one side of the positioning device 42 movably provided with a second filter 41, the positioning device 42 including a fixed plate 421 fixedly provided on one side of the mirror arm 14, a top of the fixed plate 421 fixedly provided with a side edge positioning plate 422, a fixed shaft 31 fixed to one side of the fixed plate 421 (as shown in Figure 4 ), the top of the fixed plate 421 provided with a support bottom column 423 located on both sides of the side edge positioning plate 422, both sides of the side edge positioning plate 422 fixedly provided with a baffle assembly 424, wherein the support bottom column 423 and the baffle assembly 424 are used in combination to limit the maximum range of the upward and downward movement of the filter main plate 411, thereby preventing the filter main plate 411 from interfering with the staff replacing the sample on the object platform 22.

[0061] With reference to Figure 3As shown, the second filter 41 includes a filter main plate 411 movably arranged on one side of the side positioning plate 422, and two filter side plates 412 are fixedly arranged on the two sides of the filter main plate 411 away from the side positioning plate 422. A moving groove 413 is formed on the side of the filter main plate 411 close to the side positioning plate 422, and the moving groove 413 is matched with the side positioning plate 422. A limiting baffle 414 is fixedly arranged on the side of the filter main plate 411 close to the side positioning plate 422. The limiting baffle 414, the supporting bottom column 423 and the baffle assembly 424 correspond to each other, that is, the limiting baffle 414 moves up and down between the supporting bottom column 423 and the baffle assembly 424. Further, the filter main plate 411, the filter side plate 412 and the filter main body 331 that is turned up are folded to form a filter area, and the limiting baffle 414 moves between the supporting bottom column 423 and the baffle assembly 424 to limit the moving range of the filter main plate 411. In addition to avoiding the interference of the filter main plate 411 with the staff replacing the sample on the object table 22, the top of the filter main plate 411 and the filter side plate 412 is prevented from colliding with the top connector of the mirror arm 14 to avoid damage to the filter main plate 411 and the filter side plate 412.

[0062] Specifically, the fixed plate 421 and the side positioning plate 422 are fixedly installed on one side of the mirror arm 14, and the moving groove 413 is formed on the back side of the filter main plate 411, so that the filter main plate 411 is movably installed on the side positioning plate 422. The distance between the two filter side plates 412 on the surface of the filter main plate 411 is matched with the distance between the two filter main bodies 331 on the two sides of the object table 22. When the object table 22 moves upward, the filter main body 331 turns upward around the fixed shaft 31, and the inner side of the filter main body 331 is attached to the outer side of the filter side plate 412, so that the filter main body 331 and the filter side plate 412 are combined together to block and filter harmful light such as ultraviolet light, reduce stray light and optimize imaging conditions, so that the observer can obtain clearer and more comfortable visual experience.

[0063] At the beginning, the limiting baffle 414 is on the supporting bottom column 423, which prevents the filter main plate 411 from being too close to the top surface of the object table 22, which is not convenient for the staff to place the object slide on the object table 22. When the object table 22 moves up and down, the two sides of the object table 22 will abut against the bottom of the filter side plate 412, so as to drive the filter main plate 411 to move upward together with the side positioning plate 422 until the limiting baffle 414 is attached to the baffle assembly 424. At this time, it is the maximum height of the object table 22 rising, and the baffle assembly 424 limits the rising height of the filter main plate 411 to prevent the top of the filter main plate 411 from damaging other parts on the microscope, thereby increasing the safety of the device in use.

[0064] Embodiment 4

[0065] Reference Figure 5This is the fourth embodiment of the present invention, providing an adjustable fluorescence microscope. The lifting device 25 includes a servo motor 251 mounted on a base 11. A steering gear set 252 is provided on one side of the servo motor 251, and a control button 255 is provided on the other side of the servo motor 251. A transmission screw 253 is meshed at one end of the steering gear set 252. The transmission screw 253 is movably connected to the carrying platform 22. Specifically, the carrying platform 22 is rotatably connected to the transmission screw 253, for example, by ball bearings, which is prior art. A screw top plate 254 is provided at the top of the transmission screw 253, and the screw top plate 254 is fixedly connected to the microscope arm 14. The control button 255 is used to control the switch of the servo motor 251, thereby driving the steering gear set 252 to drive the transmission screw 253 meshing with it to rotate, thereby controlling the up and down movement of the carrying platform 22 connected to the transmission screw 253. Both the control button 255 and the servo motor 251 are prior art.

[0066] Reference Figure 1 , Figure 6 The base 11 has a light source device 12 on top, which is located directly below the platform 22. A wide-angle eyepiece 15 is fixed on one side of the arm 14, and a charge coupler 16 is fixed on the top side of the wide-angle eyepiece 15. An achromatic objective lens 17 is movably mounted on the bottom of the wide-angle eyepiece 15. Focusing knobs 13 are movably mounted on both sides of the base 11 to quickly adjust the distance between the achromatic objective lens 17 and the specimen on the platform 22 so that the image is presented in the field of view. A control knob 28 is located on the bottom side of the platform 22. The control knob 28 controls the forward and backward movement of the platform 22 and the left and right movement of the specimen holder 23 on the platform 22, thereby adjusting the position of the slide so that it is directly below the achromatic objective lens 17, which facilitates the observation of the sample by the staff. The focusing knob 13 and the control knob 28 are existing technologies and will not be described in detail here.

[0067] The specimen clip 23 includes a fixed clip 231 fixedly mounted on the top of the carrying platform 22, a rotating clip 233 movably mounted on one side of the fixed clip 231, a rotating handle 234 fixedly mounted on the outer side of the rotating clip 233, and a specimen slot 232 opened on the inner side of the fixed clip 231.

[0068] Specifically, before using the fluorescence microscope, the fluorescence microscope is as follows: Figure 2As shown, then the staff first hold the rotating handle 234 to open the rotating clamp 233, and then the two sides of the object slide are clamped at the specimen clamping groove 232 of the inner side of the fixing clamp 231, then slowly release the rotating handle 234, so that the rotating clamp 233 fixes the object slide, and the inner side of the specimen clamping groove 232 and the rotating clamp 233 is inclined, and the distance between the inner side of the specimen clamping groove 232 and the rotating clamp 233 gradually decreases from bottom to top, so that the four corners of the object slide are limited inside, thereby fixing the upper side of the object slide at the same time, further increasing the fixing stability of the specimen clamp 23 to the object slide.

[0069] After the object slide is fixed, the clamping groove 26 is just located on the fixing shaft 31, and the filter main body 331 is located on one side of the limiting column 27, then press the control button 255 to start the servo motor 251, and then the servo motor 251 drives the steering gear set 252 to rotate, drives the transmission lead screw 253 to rotate in the lead screw top plate 254, and makes the object platform 22 connected with the transmission lead screw 253 outside move upward, at this time the limiting column 27 will extrude the outside of the filter main body 331, so that the filter main body 331 rotates upward around the fixing shaft 31, and the object platform 22 continues to move upward, when the filter main body 331 is completely vertical between the object platform 22 and the limiting column 27, the top of the object platform 22 just contacts the bottom of the filter side plate 412, and then drives the filter main plate 411 to move upward with the object platform 22.

[0070] Then continue to control the object platform 22 to move to the set distance, at this time stop the servo motor 251, then control the object platform 22 to move forward and backward through the control knob 28, and adjust the specimen clamp 23 to move left and right on the object platform 22, until the object slide is located directly below the achromatic objective lens 17, then the staff observes the object cell through the wide-angle ocular lens 15, and takes a picture of the object slide through the charge-coupled device 16.

[0071] The above embodiments are only used to illustrate the technical solutions of the present application, but not limit it; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that the technical solutions recorded in the foregoing embodiments can be modified, or some technical features can be replaced by equivalent ones; and these modifications or replacements will not make the essence of the corresponding technical solutions deviate from the protection scope of the technical solutions of the embodiments of the present application.

Claims

1. An adjustable fluorescence microscope comprising a base (11), a mirror arm (14) disposed on top of the base (11), a stage (22) disposed on one side of the mirror arm (14), characterized in that: The top surface of the base (11) is provided with a lifting device (25), one end of the object carrying platform (22) is movably connected with the lifting device (25); One side of the mirror arm (14) is provided with a fixed shaft (31), the fixed shaft (31) penetrates through both sides of the object carrying platform (22), a first filter (33) is rotatably arranged on the fixed shaft (31), both sides of the object carrying platform (22) are fixedly provided with limiting columns (27) for limiting the first filter (33) on both sides of the object carrying platform (22); when the object carrying platform (22) moves upward under the control of the lifting device (25), the first filter (33) is turned upward around the fixed shaft (31) under the block of the limiting columns (27), forming a filtering area, when the object carrying platform (22) moves downward, the first filter (33) is automatically turned downward around the fixed shaft (31) under the action of gravity; a through slot is formed between the limiting column (27) and the object carrying platform (22), and the first filter (33) is arranged in the through slot.

2. An adjustable fluorescence microscope according to claim 1, characterized in that: The end of the fixed shaft (31) away from the mirror arm (14) is fixedly provided with a fixing device (37), and the fixing device (37) is used for limiting and fixing the first filter (33).

3. An adjustable fluorescence microscope according to claim 1, wherein: Both sides of the bottom of the object carrying platform (22) are provided with clamping grooves (26) corresponding to the fixed shaft (31).

4. An adjustable fluorescence microscope according to claim 1, wherein: One side of the bottom of the object carrying platform (22) is provided with a control knob (28), the lifting device (25) comprises a servo motor (251) arranged on the base (11), one side of the servo motor (251) is provided with a steering gear set (252), one end of the steering gear set (252) is engaged with a transmission screw rod (253), the transmission screw rod (253) is rotatably connected with the object carrying platform (22), the top end of the transmission screw rod (253) is provided with a screw rod top plate (254), and the screw rod top plate (254) is fixedly connected with the mirror arm (14).

5. An adjustable fluorescence microscope according to claim 2, wherein: The fixing device (37) comprises a positioning partition plate (371) fixedly arranged at one end of the fixed shaft (31), a ratchet clamping column (372) movably arranged in the positioning partition plate (371), a pawl (374) fixedly arranged at one end of the ratchet clamping column (372), a torsion spring (373) arranged on the outer side of the ratchet clamping column (372), a one-way ratchet (376) rotatably arranged on the end of the fixed shaft (31) away from the mirror arm (14) and corresponding to the pawl (374), a limiting clamping column (377) fixedly arranged on one side of the one-way ratchet (376), the limiting clamping column (377) is used for abutting against the outer side of the first filter (33) to limit and fix the first filter (33), and the limiting clamping column (377) is staggered with the limiting column (27).

6. An adjustable fluorescence microscope according to claim 5, wherein: The limiting clamping column (377) comprises a connecting block fixedly connected with the one-way ratchet (376), a limiting rod fixedly arranged at the end of the connecting block away from the one-way ratchet (376), and the limiting rod extends to the outer side of the first filter (33).

7. An adjustable fluorescence microscope according to claim 1, wherein: One side of the mirror arm (14) is fixedly provided with a positioning device (42), one side of the positioning device (42) is movably provided with a second filter (41), the bottom of the second filter (41) is in contact with the object platform (22), when the object platform (22) moves up, the second filter (41) moves up with the object platform (22) and encloses the first filter (33) to form a filter area.

8. An adjustable fluorescence microscope according to claim 7, wherein: The positioning device (42) comprises a fixed plate (421) fixedly arranged on one side of the mirror arm (14), the top of the fixed plate (421) is fixedly provided with a side positioning plate (422), the top of the fixed plate (421) is provided with a supporting bottom column (423) located on both sides of the side positioning plate (422), and both sides of the side positioning plate (422) are fixedly provided with a baffle assembly (424).

9. An adjustable fluorescence microscope according to claim 8, characterized in that: The second filter (41) comprises a filter main plate (411) movably arranged on one side of the side positioning plate (422), both sides of the filter main plate (411) away from one side of the side positioning plate (422) are fixedly provided with a filter side plate (412), one side of the filter main plate (411) close to the side positioning plate (422) is provided with a moving groove (413), the moving groove (413) is matched with the side positioning plate (422), and one side of the filter main plate (411) close to the side positioning plate (422) is fixedly provided with a limiting baffle (414), the limiting baffle (414), the supporting bottom column (423) and the baffle assembly (424) correspond to each other.

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