Multi-vitamin black true treasure brewing composition as well as preparation method and application thereof

Abstract

The invention provides a multi-vitamin black true treasure brewing composition. The multi-vitamin black true treasure brewing composition is characterized by comprising puffed powder, maltodextrin, black sesame, resistant dextrin, soybean protein isolate, whole milk powder, oat, tartary buckwheat, brown rice, mulberry extract, cordyceps militaris extract, rhizoma polygonati extract, soybean meal, calcium carbonate, fructo-oligosaccharide, xylitol, multi-vitamin and sucralose. The multi-vitamin black Wubao brewing composition comprises various black cereal food materials, various traditional Chinese medicinal material extracts, vitamins and other components, and has the effects of tonifying kidney and qi, enhancing immunity and resisting fatigue.

Description

Technical Field

[0001] This application belongs to the field of health products. Specifically, this application provides a multi-dimensional black treasure brewing composition, its preparation method, and its application. Background Technology

[0002] Traditional Chinese medicine generally believes that different organs in the human body correspond to different colors. Black foods / medicines often enter the kidney meridian, which can invigorate qi and nourish the kidneys, enhance immunity, and delay aging.

[0003] Based on this understanding, there are already many food and health products on the market based on black grains. However, the effects of these foods and health products are mostly limited and cannot fully allow consumers to experience the benefits of black grain products. Therefore, there is a need in this field to continue improving black grain-based foods and health products. Summary of the Invention

[0004] To further improve the effects of black grain foods, especially instant foods, the applicant designed a multi-dimensional black grain instant composition. In addition to black grain food ingredients, it also introduced a variety of Chinese herbal extracts, vitamins and other ingredients. Furthermore, it used a suitable enzyme to modify mulberry extract, which made the composition have significant effects of tonifying the kidneys and replenishing qi, enhancing immunity and anti-fatigue.

[0005] On one hand, this application provides a multi-dimensional black sesame brewing composition, which includes puffed powder, maltodextrin, black sesame, resistant dextrin, soy protein isolate, whole milk powder, oats, buckwheat, brown rice, mulberry extract, cordyceps extract, polygonatum extract, soy flour, calcium carbonate, fructooligosaccharides, xylitol, multivitamins, and sucralose.

[0006] Furthermore, each 30g of the multi-dimensional black sesame powder preparation composition consists of 7.8g of puffed powder, 4g of maltodextrin, 2.5g of black sesame seeds, 2.5g of resistant dextrin, 2.4g of soy protein isolate, 2g of whole milk powder, 1.5g of oats, 1.5g of tartary buckwheat, 1g of brown rice, 1.5g of mulberry extract, 0.5g of cordyceps extract, 0.5g of polygonatum extract, 0.5g of soy flour, 0.5g of calcium carbonate, 0.6g of fructooligosaccharides, 0.5g of xylitol, 0.15g of complex vitamins, and 0.05g of sucralose.

[0007] Due to the large number of components, it is difficult to limit the proportions used. The above limitation uses a 30g limit (30g is the applicant's expected product unit packaging weight). Those skilled in the art will understand that due to production methods, packaging specifications, etc., other weights can be used for the composition, and the content of each component can be increased or decreased proportionally.

[0008] Furthermore, the puffed powder is prepared using black rice, rye, black beans, and black fungus in a mass ratio of 5:2:2:1.

[0009] Furthermore, the mulberry extract is an enzyme-modified mulberry water extract.

[0010] Furthermore, the mulberry extract is a water extract of mulberry modified with papain and β-glucanase, or modified with papain and dextranase.

[0011] Furthermore, the mulberry extract is prepared using the following method:

[0012] The mulberry water extract was prepared into an aqueous solution of 0.1 g / mL. Papain 500 U / mL and dextranase 700 U / mL were added to the aqueous solution, and the pH was adjusted to 5.5. The enzyme was treated at 55 degrees Celsius for 3 hours. The enzyme was then inactivated in a water bath at 90 degrees Celsius for 10 minutes. The extract was obtained by rotary evaporation and freeze drying.

[0013] On the other hand, this application provides the use of the above-mentioned multi-dimensional black treasure brewing composition in the preparation of health products or medicines that enhance immunity.

[0014] On the other hand, this application provides the application of the above-mentioned multi-dimensional black treasure brewing composition in the preparation of health products or medicines that relieve physical fatigue.

[0015] On the other hand, this application provides the application of the above-mentioned multi-dimensional black treasure brewing composition in the preparation of health products or medicines that delay aging.

[0016] On the other hand, this application provides the use of the above-mentioned multi-dimensional black treasure brewing composition in the preparation of kidney-tonifying health products or medicines.

[0017] The preparation method of the above-mentioned multi-dimensional black treasure brewing composition includes the steps of coarse grinding, compounding, fine grinding, sterilization and packaging of each raw material.

[0018] The multi-dimensional black grain health product composition of this application is rich in nutrients, contains no sucrose, is convenient to consume, and effectively expands the uses and effects of black grain health products.

[0019] The multi-dimensional black treasure brewing composition of this application can be directly brewed with warm water and consumed. Milk powder, protein powder, other health products or medicines can be added during brewing to further increase nutrition or improve taste. Attached Figure Description

[0020] Figure 1 The results of GSH measurement in each group of the D-galactose aging mouse model experiment in Example 2;

[0021] Figure 2The results of SOD activity measurement in each group of the D-galactose aging mouse model experiment in Example 2;

[0022] Figure 3 The results show the IL-6 level measurements in each group of the D-galactose aging mouse model experiment in Example 2. Detailed Implementation

[0023] The following extracts were purchased from Shaanxi Haiyis Biotechnology or prepared in-house:

[0024] Mulberry extract (20:1, water extract, total anthocyanin content 27%);

[0025] Cordyceps militaris aqueous extract (10:11, aqueous extract);

[0026] Polygonatum odoratum aqueous extract (20:11, aqueous extract);

[0027] The puffed powder, maltodextrin, black sesame seeds, resistant dextrin, soy protein isolate, whole milk powder, oats, buckwheat, brown rice, soy flour, calcium carbonate, fructooligosaccharides, xylitol, multivitamins, and sucralose were purchased from Shandong Gushuo Biotechnology, Lanzhou Waterles Biotechnology, etc., and are all food grade.

[0028] α-Amylase, β-amylase, cellulase, pectinase, β-glucanase, arabinofuranylase, dextranase, alkaline protease, papain, and trypsin were purchased from Ningxia Xiasheng and Yuanye Biotechnology.

[0029] Other reagents, raw materials and equipment are described in detail in the examples, or are of conventional types in the art.

[0030] Example 1 Enzymatic Modification of Mulberry Extract

[0031] Mulberry extract was prepared into a 0.1 g / mL aqueous solution. 700 U / mL of α-amylase, β-amylase, cellulase, pectinase, β-glucanase, arabinofuranase, and dextranase were added to the aqueous solution. The pH was adjusted to the optimal range for each enzyme (between 5 and 9, not excessively acidic or alkaline). The enzymes were treated at 55°C for 3 hours; then the enzymes were inactivated in a 90°C water bath for 10 minutes; finally, the modified mulberry extract was obtained by rotary evaporation and freeze-drying.

[0032] The DPPH free radical scavenging rate and reducing power of the original mulberry extract and the modified extract (50 mg / mL aqueous solution) were detected using a kit from Shanghai Yanqi Biotechnology. The results are shown in Table 1 (average of three samples).

[0033] Table 1. Antioxidant properties of polysaccharide-enzyme-modified mulberry extract

[0034]

[0035]

[0036] The results showed that, similar to those of existing technologies, enzymatic modification with most polysaccharide enzymes was detrimental to the antioxidant properties of mulberry extract, possibly because the antioxidant capacity of mulberry polysaccharides was destroyed during the modification process. Only pectinase (pH 5), β-glucanase (pH 8), and dextranase (pH 5) showed positive effects.

[0037] Furthermore, considering the protein components in mulberries and the results of preliminary experiments, as well as the pH range of commonly used proteases, the following enzymes were used to enzymatically modify mulberry extract: alkaline protease 500 U / mL + β-glucanase 700 U / mL (pH 8.5), papain 500 U / mL + pectinase 700 U / mL (pH 5.5), papain 500 U / mL + β-glucanase 700 U / mL (pH 7.5), papain 500 U / mL + dextranase 700 U / mL (pH 5.5), and trypsin 500 U / mL + β-glucanase 700 U / mL (pH 7.5) (basic method as above). The results are shown in Table 2.

[0038] Table 2. Antioxidant properties of mulberry extract modified with compound enzymes

[0039]

[0040] The results showed that only the papain + β-glucanase and papain + dextranase combinations achieved positive results, while the effects of other complex enzymes were not as good as those of single polysaccharide enzymes.

[0041] Example 2: Effects in a D-galactose-induced aging mouse model

[0042] Fifty healthy male BALB / c mice (20g±2g) were randomly divided into 5 groups and treated as follows:

[0043] Positive control: Subcutaneous injection of D-gal 8 mg / day for 6 weeks;

[0044] Negative control: daily subcutaneous injection of normal saline for 6 weeks;

[0045] Raw mulberry extract, D-gal 8mg subcutaneously daily for 6 weeks; from the 8th day onwards, 50mg extract daily by gavage.

[0046] Papain + β-glucanase modified mulberry extract, D-gal 8mg subcutaneously daily for 6 weeks; from the 8th day onwards, 50mg modified extract by gavage daily.

[0047] Papain + dextranase modified mulberry extract, D-gal 8mg subcutaneously daily for 6 weeks; from the 8th day onwards, 50mg modified extract by gavage daily.

[0048] After the 6-week experiment, the serum GSH and SOD activity levels were detected using kits from Shanghai Yanqi Biotechnology and Shanghai Xuanzekang Biotechnology; the serum IL-6 level was detected using a kit from Wuhan Aidikang (IL-6 is generally considered to be associated with diseases such as diabetes, cognitive impairment, and various tumors, and its level gradually increases during aging, serving as one of the markers of aging in mice).

[0049] The results are as follows Figure 1 As shown, compared with the negative control, the levels of GSH and SOD in the positive control were significantly decreased, while the level of IL-6 was significantly increased, indicating that the model was successfully established. Compared with papain + β-glucanase, papain + dextranase can more effectively reverse the above changes. Therefore, papain + dextranase modified mulberry extract was selected for further practical application.

[0050] Example 3: Formulation and preparation of the multi-dimensional black genuine treasure brewing composition

[0051] Using the enzyme-modified mulberry extract from Example 2, the multi-dimensional Black Treasure brewing composition was prepared according to the formulation in the table below:

[0052] Table 3 Multi-Dimensional Black Treasure Formula

[0053]

[0054]

[0055] Each raw material is coarsely ground, compounded, finely ground, sterilized, and packaged separately.

[0056] This product can be brewed with warm water. Milk powder, protein powder, etc. can be added during brewing to further increase nutrition or improve the taste.

[0057] Example 4: The effects of the multi-dimensional black genuine treasure brewing composition on relieving fatigue and enhancing immunity in mice.

[0058] Thirty healthy male BALB / c mice weighing 20±2g were randomly divided into three groups and acclimatized in the experimental environment for one week.

[0059] The control group was fed normally. The control group and the multivitamin Black Treasure group swam to exhaustion once in the morning and once in the afternoon each day (at a water temperature of 25 degrees Celsius), with their day and night cycles reversed (rotating every 48 hours); they were given sufficient normal powdered rodent food and drinking water; the multivitamin Black Treasure group had 1 / 3 of their weight of multivitamin Black Treasure mixed into their rodent food.

[0060] For 30 consecutive days (with no deaths or obvious abnormal activity during this period), the mice were tested for exhaustive swimming time on day 31. After sacrifice, their internal organs were removed, and the spleen and thymus coefficients were calculated.

[0061] Table 4 Results of exhaustive swimming time, spleen coefficient, and thymus coefficient in mice

[0062]

[0063]

[0064] The results showed that Multivitamin Black Treasure significantly alleviated fatigue in mice and maintained their immunity.

Claims

1. A multidimensional black sesame brewing composition, characterized in that, The multi-dimensional black sesame powder preparation composition contains puffed powder, maltodextrin, black sesame seeds, resistant dextrin, soy protein isolate, whole milk powder, oats, buckwheat, brown rice, mulberry extract, cordyceps extract, polygonatum extract, soy flour, calcium carbonate, fructooligosaccharides, xylitol, multivitamins, and sucralose.

2. The multivitamin black sesame powder preparation composition according to claim 1, wherein each 30g of the multivitamin black sesame powder preparation composition comprises 7.8g of puffed powder, 4g of maltodextrin, 2.5g of black sesame seeds, 2.5g of resistant dextrin, 2.4g of soy protein isolate, 2g of whole milk powder, 1.5g of oats, 1.5g of tartary buckwheat, 1g of brown rice, 1.5g of mulberry extract, 0.5g of cordyceps militaris extract, 0.5g of polygonatum extract, 0.5g of soy flour, 0.5g of calcium carbonate, 0.6g of fructooligosaccharides, 0.5g of xylitol, 0.15g of complex vitamins, and 0.05g of sucralose.

3. The multidimensional black gluten powder preparation composition according to claim 1, wherein the puffed powder is prepared using black rice, rye, black beans and black fungus in a mass ratio of 5:2:2:

1.

4. The multi-dimensional black mulberry brewing composition according to any one of claims 1-3, wherein the mulberry extract is an enzyme-modified mulberry water extract.

5. The multidimensional black mulberry brewing composition according to claim 4, wherein the mulberry extract is a mulberry water extract modified by papain and β-glucanase, or modified by papain and dextranase.

6. The multidimensional black mulberry extract infusion composition according to claim 5, wherein the mulberry extract is prepared using the following method: The mulberry water extract was prepared into an aqueous solution of 0.1 g / mL. Papain 500 U / mL and dextranase 700 U / mL were added to the aqueous solution, and the pH was adjusted to 5.

5. The enzyme was treated at 55 degrees Celsius for 3 hours, and then the enzyme was inactivated in a water bath at 90 degrees Celsius for 10 minutes. The extract was obtained by rotary evaporation and freeze drying.

7. The use of the multi-dimensional black treasure brewing composition according to any one of claims 1-6 in the preparation of health products or pharmaceuticals that enhance immunity.

8. The use of the multi-dimensional black sesame brewing composition according to any one of claims 1-6 in the preparation of health products or medicines for relieving physical fatigue.

9. The use of the multi-dimensional black sesame brewing composition according to any one of claims 1-6 in the preparation of health products or medicines for delaying aging.

10. The use of the multi-dimensional black sesame powder composition according to any one of claims 1-6 in the preparation of kidney-tonifying health products or medicines.

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