High-efficiency antibacterial mite-killing spray and preparation method thereof

Abstract

The invention provides an efficient antibacterial and mite-killing spray and a preparation method of the efficient antibacterial and mite-killing spray. The spray is prepared from the following components: 0.1 to 0.5 percent of tetrasodium glutamate diacetate, 0.5 to 1.5 percent of lauryl amine dipropylene diamine, 0.1 to 0.5 percent of dichlorobenzyl alcohol, 0.2 to 2.0 percent of PEG-40 hydrogenated castor oil, 0.5 to 2.0 percent of persimmon extract, 0.5 to 1.5 percent of soybean ethyl sulfate morpholine, 0.2 to 1.0 percent of dodecyl dimethyl benzyl ammonium chloride, 0 to 0.5 percent of lactobacillus / rye fine powder fermentation product, 1 to 5 percent of glycerol, 0.5 to 1.0 percent of ethylhexylglycine, 0 to 1.0 percent of lactic acid and the balance of water. 0 to 0.3 percent of essence; and 82.7 to 96.9 percent of deionized water. A unique odor control technology is adopted to be compounded with the persimmon extract, odor molecules are decomposed from the source at a multiple speed, and the environment is purified. The mite-killing spray has the effects of removing odor, inhibiting bacteria, killing mites and balancing micro-ecology, and pets and the surrounding environment can be cared and cared in a multi-effect manner.

Description

Technical Field

[0001] This invention relates to the field of spray technology, specifically to a highly efficient antibacterial and anti-mite spray and its preparation method. Background Technology

[0002] Pet sprays are products specifically designed for pet care, primarily used for pet skin care, environmental disinfection, and odor reduction. Pet deodorizing and antibacterial sprays are mainly used to eliminate odors on pets and kill bacteria, protecting the health of pets and their families. They contain deodorants, bactericides, and plant extracts, eliminating odors through physical adsorption, chemical neutralization, or biodegradation, and killing bacteria and viruses through bactericides. Chinese invention patent application CN110314128A discloses a pet shampoo with insecticidal function, belonging to the field of daily necessities technology. It is prepared from the following components in parts by weight: fipronil 0.5-5 parts, pyrethrin 0.5-2 parts, antibacterial agent 0.01-0.3 parts, fresh ginger juice extract 15-25 parts, motherwort extract 5-10 parts, codonopsis pilosula 5-8 parts, angelica sinensis powder 5-8 parts, honey 10-18 parts, beer 18-25 parts, surfactant 20-30 parts, chelating agent 3-5 parts, preservative 1-2 parts, and rose fragrance 0.1-0.5 parts.

[0003] Regarding the aforementioned technologies, the inventors believe that the following defects exist: Although fipronil and pyrethrum are effective killing agents for mites and other pests, and have obvious killing effects, fipronil accounts for a relatively high proportion. Pets have a natural instinct to lick their fur, and high levels of fipronil can easily interfere with the chloride ion channels of the nervous system, causing excessive excitation of the central nervous system, leading to convulsions, ataxia, or even coma in pets. Therefore, it is necessary to develop a spray that has good killing effect on mites and other pests and has good safety. Summary of the Invention

[0004] To address the problems existing in the prior art, this invention provides a highly efficient antibacterial and anti-mite spray and its preparation method.

[0005] To achieve the above objectives, the present invention provides the following technical solution: This application discloses a highly effective antibacterial and anti-mite spray. The spray comprises, by weight percentage: 0.1-0.5% tetrasodium glutamate diacetate, 0.5-1.5% laurylamine dipropylenediamine, 0.1-0.5% dichlorobenzyl alcohol, 0.2-2.0% PEG-40 hydrogenated castor oil, 0.5-2.0% persimmon extract, 0.5-1.5% soybean ethyl sulfate morpholine, 0.2-1.0% dodecyl dimethyl benzyl ammonium chloride, 0-0.5% Lactobacillus / rye flour fermentation product, 1-5% glycerol, 0.5-1.0% ethylhexyl glycerin, 0-1.0% lactic acid, 0-0.3% fragrance, and 82.7-96.9% deionized water.

[0006] Preferably, the spray composition, by weight percentage, is as follows: 0.30% tetrasodium glutamate diacetate, 1.00% laurylamine dipropylenediamine, 0.15% dichlorobenzyl alcohol, 0.60% PEG-40 hydrogenated castor oil, 1.50% persimmon extract, 1.00% soybean ethyl sulfate morpholine, 0.50% dodecyl dimethyl benzyl ammonium chloride, 0.15% Lactobacillus / rye flour fermentation product, 3.00% glycerin, 0.80% ethylhexyl glycerin, 0.20% lactic acid, 0.10% fragrance, and 90.70% deionized water.

[0007] By employing the above technical solution, tetrasodium glutamate diacetate acts as a chelating agent to help stabilize the product. Dodecyl dimethyl benzyl ammonium chloride, as a cationic surfactant, directly disrupts microbial cell membranes, and its chelating effect with tetrasodium glutamate diacetate synergistically blocks metal-dependent bacterial metabolic pathways (metal ions required for microbial physiology, such as Ca2+). 2+ Mg 2+This combination creates a broad-spectrum bactericidal effect. Dichlorobenzyl alcohol, as an auxiliary acaricide, interferes with the metabolic enzyme activity of mites by damaging their epidermal lipid layer or penetrating into their bodies, leading to dehydration and death. Laurylamine dipropylenediamine, acting as a surfactant and acaricide, enhances the permeability of dichlorobenzyl alcohol, allowing it to penetrate the mite's surface barrier more efficiently. It may also accelerate the killing process by dissolving the lipids in the mite's epidermis. Furthermore, dichlorobenzyl alcohol directly dissolves the lipid layer of the mite's epidermis, disrupting its moisture retention capacity, while laurylamine dipropylenediamine further expands the extent of epidermal damage through emulsification, accelerating mite dehydration. Dichlorobenzyl alcohol can inhibit key enzymes (such as digestive enzymes) in mites, while laurylamine dipropylenediamine blocks their physiological activities by interfering with their calcium ion channels or nerve conduction systems, creating a dual lethal effect. PEG-40 hydrogenated castor oil, acting as a solubilizer, promotes uniform mixing of fragrance, dichlorobenzyl alcohol, persimmon extract, and the aqueous phase, enhancing the stability and aroma of the bactericide. The persimmon extract and soybean ethyl sulfate morpholine complex can form a complex, altering the chemical structure or stability of odor molecules, thereby reducing odor intensity. Furthermore, the combination reduces skin irritation to pets and enhances environmental friendliness. Dichlorobenzyl alcohol, by interfering with cellular metabolic enzyme activity, forms a dual bactericidal target with dodecyl dimethyl benzyl chloride. Glycerin, acting as a co-solvent and humectant, not only helps dissolve dichlorobenzyl alcohol but also reduces the dryness and irritation to pet skin. Ethylhexylglycerin, acting as a preservative synergist, prolongs the duration of antibacterial activity. Lactic acid, acting as a pH adjuster, adjusts the system pH to a slightly acidic level (close to the natural pH of pet skin), enhancing the bactericidal activity of dodecyl dimethyl benzyl chloride while inhibiting fungal growth.

[0008] Preferably, the method for preparing the persimmon extract includes the following steps: A1. Take fresh, good-quality persimmons, remove impurities and wash them more than 3 times, cut them into thin slices and put them into the extraction tank. Add water at 6-8 times the weight of the persimmons, heat to 85℃, keep warm and extract for 2 hours, filter and retain the filtrate. Add the same amount of water as the first extraction to the filter residue and repeat the extraction for 2 hours. Combine the two filtrates. A2. Transfer the filtrate to a single-effect concentrator, control the vacuum degree to 0.06-0.08MPa, the concentration temperature to 60-80℃, and the concentration time to 8-10h. A3. The concentrated juice is transferred to a spray drying tower for spray drying and powdering to obtain powdered persimmon extract.

[0009] By setting up the above technical solution, both persimmon extract and soybean ethyl ethoxylate morpholine (a soybean derivative) are used as deodorizers. The tannins and other components in the persimmon extract may capture odor molecules through adsorption, while soybean ethyl ethoxylate morpholine decomposes volatile odor substances such as sulfides through chemical neutralization reaction, thus achieving synergistic odor removal.

[0010] Preferably, during the spray drying stage, the inlet air temperature is adjusted to 150-165℃, the outlet air temperature to 65-85℃, the tower internal temperature to 75-90℃, and the negative pressure to 10-18Pa.

[0011] Preferably, the method for preparing the Lactobacillus / rye flour fermentation product includes the following steps: B1. Select high-quality rye, grind it finely, pass it through a 100-mesh sieve, and mix it evenly with water at a ratio of 1:1.2-1:1.5 to form a rye suspension. Let it stand for 30 minutes. B2. After sterilizing the rye suspension at high temperature, quickly cool it to 36-37℃. Take the lyophilized lactobacillus powder, reconstitute it with sterile physiological saline, and add it to the sterilized rye suspension at an inoculation rate of 3-5%. Ferment for 30-36 hours under low-speed stirring. B3. Centrifuge the fermentation product obtained in B2 at 4000 r / min for 13-15 min to obtain the fermentation broth. Pasteurize the fermentation broth and then dry the fermentation product in a drying oven at 55-58℃ until the moisture content is 10%-12%. Crush the product to obtain the Lactobacillus / rye flour fermentation product.

[0012] By setting up the above technical solution, the fermentation products of lactobacillus / rye flour provide prebiotic components, maintain the balance of the pet's skin surface flora, inhibit the colonization of harmful bacteria, and form a three-dimensional protection of "antibacterial + antimicrobial" with dodecyl dimethyl benzyl ammonium chloride.

[0013] Preferably, the high-temperature sterilization temperature is 123-125℃, and the low-speed stirring speed is 100-110 r / min.

[0014] Preferably, in step B3, the pasteurization temperature is 72°C and the time is 20 seconds.

[0015] This application also discloses a method for preparing a highly effective antibacterial and anti-mite spray, which includes the following steps: S1. Add deionized water to a mixing tank and heat to 30-35℃. Add tetrasodium diacetate of glutamic acid while stirring, and stir for 3-5 minutes until it is completely dissolved. S2. Mix dichlorobenzyl alcohol, fragrance, glycerin, PEG-40 hydrogenated castor oil, and ethylhexyl glycerin to form a transparent solution; S3. Add laurylamine dipropylene diamine, soybean ethyl sulfate morpholine, and dodecyl dimethyl benzyl ammonium chloride to the solution obtained in S1, and stir for 10-15 minutes to completely dissolve it. S4. While stirring, add the mixture obtained in S2 to the solution obtained in S3, and stir for 15-25 minutes until it is completely dissolved. S5. While stirring, add lactic acid, persimmon extract and lactobacillus / rye flour fermentation product to the solution obtained in S4, stir for 10-15 minutes, and adjust the pH to slightly acidic. S6. Filter the material through an 800-850 mesh filter, fill it, and you will get a high-efficiency antibacterial and anti-mite spray.

[0016] Preferably, the pH of the solution obtained in step S5 is 4.0-7.0.

[0017] Preferably, the pH of the solution obtained in step S5 is 5.9.

[0018] The beneficial effects of this invention are as follows: 1. The mite-removing spray obtained by this invention has the functions of deodorizing, antibacterial, mite removal, and balancing the microecology, providing multi-effect and safe protection for pets and the surrounding environment.

[0019] 2. The mite-removing spray obtained by this invention uses a unique odor control technology combined with persimmon extract to rapidly break down odor molecules at the source and purify the environment.

[0020] 3. The mite-removing spray obtained by this invention has antibacterial effects against Escherichia coli, Staphylococcus aureus, etc., and is actually non-toxic and non-irritating to the skin.

[0021] 4. The mite-removing spray obtained by this invention is a plant-based compound mite-removing agent, which is natural, safe, and has multiple synergistic effects to effectively remove mites and reduce their breeding. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Example 1: This embodiment discloses a highly efficient antibacterial and anti-mite spray. The spray's components, by weight percentage, are as follows: 0.5% tetrasodium glutamate diacetate, 1.5% laurylamine dipropylenediamine, 0.5% dichlorobenzyl alcohol, 2.0% PEG-40 hydrogenated castor oil, 2.0% persimmon extract, 1.5% soybean ethyl sulfate morpholine, 1.0% dodecyl dimethyl benzyl ammonium chloride, 0.5% Lactobacillus / rye flour fermentation product, 5% glycerol, 1.0% ethylhexyl glycerin, 1.0% lactic acid, 0.3% fragrance, and 83.2% deionized water.

[0024] The preparation method of persimmon extract includes the following steps: A1. Take fresh, high-quality persimmons, remove impurities and wash them more than 3 times, cut them into thin slices and put them into an extraction tank. Add water at 6 times the weight of the persimmons, heat to 85℃, keep warm and extract for 2 hours, filter and retain the filtrate. Add the same amount of water as the first extraction to the filter residue and repeat the extraction for 2 hours. Combine the two filtrates. A2. Transfer the filtrate to a single-effect concentrator, control the vacuum degree to 0.06MPa, the concentration temperature to 60℃, and the concentration time to 8h. A3. Transfer the concentrated juice to a spray drying tower for spray drying and powdering to obtain powdered persimmon extract; The inlet air temperature is 150℃, the outlet air temperature is 65℃, the internal temperature of the tower is 75℃, and the negative pressure is 10Pa.

[0025] The preparation method of Lactobacillus / rye flour fermentation product includes the following steps: B1. Select high-quality rye, grind it finely, pass it through a 100-mesh sieve, and mix it evenly with water at a mass ratio of 1:1.2 to form a rye suspension. Let it stand for 30 minutes. B2. Sterilize the rye suspension at a high temperature of 123℃, then rapidly cool it to 36℃. Reconstitute the lyophilized lactobacillus powder with sterile physiological saline, add it to the sterilized rye suspension at an inoculation rate of 3%, and stir at a low speed of 100r / min for 30h. B3. Centrifuge the fermentation product obtained in B2 at 4000 r / min for 13 min to obtain the fermentation broth. Pasteurize the fermentation broth at 72℃ for 20 s. Then dry the fermentation product in a drying oven at 55℃ until the moisture content is 10%. Crush the product to obtain the Lactobacillus / rye flour fermentation product.

[0026] This embodiment also discloses a method for preparing a highly effective antibacterial and anti-mite spray, which includes the following steps: S1. Add deionized water to a mixing tank and heat to 30°C. Add tetrasodium glutamate diacetate while stirring and stir for 3 minutes until it is completely dissolved. S2. Mix dichlorobenzyl alcohol, fragrance, glycerin, PEG-40 hydrogenated castor oil, and ethylhexyl glycerin to form a transparent solution; S3. Add laurylamine dipropylene diamine, soybean ethyl sulfate morpholine, and dodecyl dimethyl benzyl ammonium chloride to the solution obtained in S1, and stir for 10 minutes to completely dissolve it. S4. While stirring, add the mixture obtained in S2 to the solution obtained in S3, and stir for 15 minutes to completely dissolve it. S5. While stirring, add lactic acid, persimmon extract and lactobacillus / rye flour fermentation product to the solution obtained in S4, stir for 10 min, and adjust the pH to 4.0. S6. Filter the material through an 800-mesh filter, fill it, and you will get a high-efficiency antibacterial and anti-mite spray.

[0027] Example 2: This embodiment discloses a highly efficient antibacterial and anti-mite spray. The components of the spray, by weight percentage, are as follows: 0.1% tetrasodium glutamate diacetate, 0.5% laurylamine dipropylenediamine, 0.1% dichlorobenzyl alcohol, 0.2% PEG-40 hydrogenated castor oil, 0.5% persimmon extract, 0.5% soybean ethyl sulfate morpholine, 0.2% dodecyl dimethyl benzyl ammonium chloride, 1% glycerol, 0.5% ethylhexyl glycerin, and 96.4% deionized water.

[0028] The preparation method of persimmon extract includes the following steps: A1. Take fresh, high-quality persimmons, remove impurities and wash them more than 3 times, cut them into thin slices and put them into an extraction tank. Add water at 8 times the weight of the persimmons, heat to 85℃, keep warm and extract for 2 hours, filter and retain the filtrate. Add the same amount of water as the first extraction to the filter residue and extract again for 2 hours. Combine the two filtrates. A2. Transfer the filtrate to a single-effect concentrator, control the vacuum degree to 0.08MPa, the concentration temperature to 80℃, and the concentration time to 10h. A3. Transfer the concentrated juice to a spray drying tower for spray drying and powdering to obtain powdered persimmon extract; The inlet air temperature is 165℃, the outlet air temperature is 85℃, the internal temperature of the tower is 90℃, and the negative pressure is 18Pa.

[0029] This embodiment also discloses a method for preparing a highly effective antibacterial and anti-mite spray, which includes the following steps: S1. Add deionized water to a mixing tank and heat to 35°C. Add tetrasodium diacetate of glutamic acid while stirring, and stir for 5 minutes until it is completely dissolved. S2. Mix dichlorobenzyl alcohol, glycerol, PEG-40 hydrogenated castor oil, and ethylhexyl glycerin to form a transparent solution; S3. Add laurylamine dipropylene diamine, soybean ethyl sulfate morpholine, and dodecyl dimethyl benzyl ammonium chloride to the solution obtained in S1, and stir for 15 minutes to completely dissolve it. S4. While stirring, add the mixture obtained in S2 to the solution obtained in S3, and stir for 25 minutes to completely dissolve it. S5. While stirring, add persimmon extract to the solution obtained in S4, stir for 15 minutes, and adjust the pH to 7.0. S6. Filter the material through an 850-mesh filter, fill it, and you will get a high-efficiency antibacterial and anti-mite spray.

[0030] Example 3: This embodiment discloses a highly efficient antibacterial and anti-mite spray. The spray's components, by weight percentage, are as follows: 0.30% tetrasodium glutamate diacetate, 1.00% laurylamine dipropylenediamine, 0.15% dichlorobenzyl alcohol, 0.60% PEG-40 hydrogenated castor oil, 1.50% persimmon extract, 1.00% soybean ethyl sulfate morpholine, 0.50% dodecyl dimethyl benzyl ammonium chloride, 0.15% Lactobacillus / rye flour fermentation product, 3.00% glycerin, 0.80% ethylhexyl glycerin, 0.20% lactic acid, 0.10% fragrance, and 90.70% deionized water.

[0031] The preparation method of persimmon extract includes the following steps: A1. Take fresh, high-quality persimmons, remove impurities and wash them more than 3 times, cut them into thin slices and put them into an extraction tank. Add water at 7 times the weight of the persimmons, heat to 85℃, keep warm and extract for 2 hours, filter and retain the filtrate. Add the same amount of water as the first extraction to the filter residue and extract again for 2 hours. Combine the two filtrates. A2. Transfer the filtrate to a single-effect concentrator, control the vacuum degree to 0.07MPa, the concentration temperature to 70℃, and the concentration time to 9h. A3. Transfer the concentrated juice to a spray drying tower for spray drying and powdering to obtain powdered persimmon extract; The inlet air temperature is 157℃, the outlet air temperature is 75℃, the internal temperature of the tower is 82℃, and the negative pressure is 14Pa.

[0032] The preparation method of Lactobacillus / rye flour fermentation product includes the following steps: B1. Select high-quality rye, grind it finely, pass it through a 100-mesh sieve, and mix it evenly with water at a mass ratio of 1:1.3 to form a rye suspension. Let it stand for 30 minutes. B2. Sterilize the rye suspension at a high temperature of 124℃, then rapidly cool it to 36.5℃. Reconstitute the lyophilized lactobacillus powder with sterile physiological saline, add it to the sterilized rye suspension at an inoculation rate of 4%, and stir at a low speed of 105r / min for 33h. B3. Centrifuge the fermentation product obtained in B2 at 4000 r / min for 14 min to obtain the fermentation broth. Pasteurize the fermentation broth at 72℃ for 20 s. Then dry the fermentation product in a drying oven at 56℃ until the moisture content is 11%. Crush the product to obtain the Lactobacillus / rye flour fermentation product.

[0033] This embodiment also discloses a method for preparing a highly effective antibacterial and anti-mite spray, which includes the following steps: S1. Add deionized water to a mixing tank and heat to 32°C. Add tetrasodium diacetate of glutamic acid while stirring, and stir for 4 minutes until it is completely dissolved. S2. Mix dichlorobenzyl alcohol, fragrance, glycerin, PEG-40 hydrogenated castor oil, and ethylhexyl glycerin to form a transparent solution; S3. Add laurylamine dipropylene diamine, soybean ethyl sulfate morpholine, and dodecyl dimethyl benzyl ammonium chloride to the solution obtained in S1, and stir for 12 minutes to completely dissolve it. S4. While stirring, add the mixture obtained in S2 to the solution obtained in S3, and stir for 20 minutes to completely dissolve it. S5. While stirring, add lactic acid, persimmon extract and lactobacillus / rye flour fermentation product to the solution obtained in S4, stir for 12 min, and adjust the pH to 5.9. S6. Filter the material through an 825-mesh filter, fill it, and you will get a high-efficiency antibacterial and anti-mite spray.

[0034] Comparative Example 1: A highly effective antibacterial and anti-mite spray, the only difference between this spray and Example 3 is that it does not contain tetrasodium diglutamate.

[0035] Comparative Example 2: A highly effective antibacterial and anti-mite spray, the only difference between this spray and Example 3 is that laurylamine dipropylenediamine is not added.

[0036] Comparative Example 3: A highly effective antibacterial and anti-mite spray, the only difference between this spray and Example 3 is that dichlorobenzyl alcohol is not added.

[0037] Comparative Example 4: A highly effective antibacterial and anti-mite spray, which differs from Example 3 only in that it does not contain PEG-40 hydrogenated castor oil.

[0038] Comparative Example 5: A highly effective antibacterial and anti-mite spray, the only difference between this spray and Example 3 is that no persimmon extract is added.

[0039] Comparative Example 6: A highly effective antibacterial and anti-mite spray, the only difference between this spray and Example 3 is that persimmon powder is used instead of persimmon extract.

[0040] Comparative Example 7: A highly effective antibacterial and anti-mite spray, the only difference between this spray and Example 3 is that it does not contain soybean ethyl sulfate morpholine.

[0041] Comparative Example 8: A highly effective antibacterial and anti-mite spray, the only difference between this spray and Example 3 is that: no dodecyl dimethyl benzyl ammonium chloride is added.

[0042] Comparative Example 9: A highly effective antibacterial and anti-mite spray, the only difference between this spray and Example 3 is that it does not contain lactobacillus / rye flour fermentation products.

[0043] Comparative Example 10: A highly effective antibacterial and anti-mite spray, the only difference between this spray and Example 3 is that fine rye flour is used instead of Lactobacillus / fine rye flour fermentation product.

[0044] Comparative Example 11: A highly effective antibacterial and anti-mite spray, which differs from Example 3 only in that it does not contain ethylhexyl glycerin.

[0045] Comparative Example 12: A highly effective antibacterial and anti-mite spray, which differs from Example 3 only in that it does not contain lactic acid.

[0046] Comparative Example 13: A highly effective antibacterial and anti-mite spray, the only difference between this spray and Example 3 is that no fragrance is added.

[0047] The original spray solutions obtained in Examples 1-3 and Comparative Examples 1-13 were sent to a professional testing institution for testing of Staphylococcus aureus inhibition rate, Escherichia coli inhibition rate, mite killing rate, and ammonia removal rate.

[0048] The concentration of Staphylococcus aureus was 2.2 × 10⁻⁶. 6 The concentration of CFU / mL and E. coli was 2.8 × 10⁻⁶. 6CFU / mL. During the test, Staphylococcus aureus / Escherichia coli strains were sprayed with the undiluted spray solution of each example and each comparative example for 5 minutes, and the inhibition rate of each strain was calculated.

[0049] Twenty KM mice, half female and half male, were used. Oral toxicity tests were conducted on different mice using the undiluted spray solution of each example and comparative example under an environment of 20-26℃ and 30%-70% relative humidity. The extra mice were used as blank controls for oral toxicity tests with water (the amount of spray and water used in all experimental groups was the same).

[0050] According to TCIAA017-2022 "Test Method and Evaluation of Anti-mite and Anti-mite Performance of Household Cleaning Products", the anti-mite performance of the sprays in each embodiment and comparative example was tested. Specifically, the test was conducted at a temperature of 25.1℃ and a relative humidity of 85%. The sprays in each embodiment and comparative example were diluted with water at a ratio of 1:10 and placed in different petri dishes. 200 dust mites were placed in each petri dish and observed for 30 minutes. The mortality of the dust mites was then examined under a microscope.

[0051] According to the test method of QB / T2761-2006, the sprays of each embodiment and comparative example were used to test 1.5m... 3 The removal rate of ammonia was tested in different environmental chambers with an action time of 24 hours.

[0052] The sprays obtained in Examples 1-3 and Comparative Examples 1-13 were subjected to performance tests, and the comprehensive performance of the sprays was measured. The results are shown in Table 1.

[0053] Table 1

[0054] As shown in Table 1: The sprays obtained in each embodiment and comparative example all showed an inhibition rate of >50% against Staphylococcus aureus and Escherichia coli, which meets the requirements of Q / SNDA003-2024.

[0055] The sprays obtained in each embodiment and comparative example showed no practical toxicity to the acute oral toxicity of male and female mice, meeting the requirements of Q / SNDA003-2024.

[0056] The sprays obtained in each embodiment of the present invention have a killing efficiency of ≥90% against dust mites, and are qualified mite-removing sprays.

[0057] The absence of tetrasodium diacetate, dichlorobenzyl alcohol, dodecyl dimethyl benzyl ammonium chloride, and the absence of Lactobacillus / rye flour fermentation products or the use of rye flour as a substitute for Lactobacillus / rye flour fermentation products all significantly affect the antibacterial effect and mite removal rate of the mite-removing spray.

[0058] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A highly effective antibacterial and anti-mite spray, characterized in that, The composition of this spray, by weight percentage, is as follows: 0.1-0.5% tetrasodium glutamate diacetate, 0.5-1.5% laurylamine dipropylenediamine, 0.1-0.5% dichlorobenzyl alcohol, 0.2-2.0% PEG-40 hydrogenated castor oil, 0.5-2.0% persimmon extract, 0.5-1.5% soybean ethyl ethyl sulfate morpholine, 0.2-1.0% dodecyl dimethyl benzyl ammonium chloride, 0-0.5% Lactobacillus / rye flour fermentation product, 1-5% glycerin, 0.5-1.0% ethylhexyl glycerin, 0-1.0% lactic acid, 0-0.3% fragrance, and 82.7-96.9% deionized water.

2. The high-efficiency antibacterial and anti-mite spray according to claim 1, characterized in that, The composition of this spray, by weight percentage, is as follows: 0.30% tetrasodium glutamate diacetate, 1.00% laurylamine dipropylenediamine, 0.15% dichlorobenzyl alcohol, 0.60% PEG-40 hydrogenated castor oil, 1.50% persimmon extract, 1.00% soybean ethyl sulfate morpholine, 0.50% dodecyl dimethyl benzyl ammonium chloride, 0.15% Lactobacillus / rye milling fermentation product, 3.00% glycerin, 0.80% ethylhexyl glycerin, 0.20% lactic acid, 0.10% fragrance, and 90.70% deionized water.

3. The high-efficiency antibacterial and anti-mite spray according to claim 1 or 2, characterized in that, The preparation method of the persimmon extract includes the following steps: A1. Take fresh, good-quality persimmons, remove impurities and wash them more than 3 times, cut them into thin slices and put them into the extraction tank. Add water at 6-8 times the weight of the persimmons, heat to 85℃, keep warm and extract for 2 hours, filter and retain the filtrate. Add the same amount of water as the first extraction to the filter residue and repeat the extraction for 2 hours. Combine the two filtrates. A2. Transfer the filtrate to a single-effect concentrator, control the vacuum degree to 0.06-0.08MPa, the concentration temperature to 60-80℃, and the concentration time to 8-10h. A3. The concentrated juice is transferred to a spray drying tower for spray drying and powdering to obtain powdered persimmon extract.

4. The high-efficiency antibacterial and anti-mite spray according to claim 3, characterized in that, During the spray drying stage, adjust the inlet air temperature to 150-165℃, the outlet air temperature to 65-85℃, the tower internal temperature to 75-90℃, and the negative pressure to 10-18Pa.

5. The high-efficiency antibacterial and anti-mite spray according to claim 1 or 2, characterized in that, The preparation method of the Lactobacillus / rye flour fermentation product includes the following steps: B1. Select high-quality rye, grind it finely, pass it through a 100-mesh sieve, and mix it evenly with water at a ratio of 1:1.2-1:1.5 to form a rye suspension. Let it stand for 30 minutes. B2. After sterilizing the rye suspension at high temperature, quickly cool it to 36-37℃. Take the lyophilized lactobacillus powder, reconstitute it with sterile physiological saline, and add it to the sterilized rye suspension at an inoculation rate of 3-5%. Ferment for 30-36 hours under low-speed stirring. B3. Centrifuge the fermentation product obtained in B2 at 4000 r / min for 13-15 min to obtain the fermentation broth. Pasteurize the fermentation broth and then dry the fermentation product in a drying oven at 55-58℃ until the moisture content is 10%-12%. Crush the product to obtain the Lactobacillus / rye flour fermentation product.

6. The high-efficiency antibacterial and anti-mite spray according to claim 5, characterized in that, The high-temperature sterilization temperature is 123-125℃, and the low-speed stirring speed is 100-110r / min.

7. The high-efficiency antibacterial and anti-mite spray according to claim 5, characterized in that, In step B3, the pasteurization temperature is 72°C and the time is 20 seconds.

8. A method for preparing a highly effective antibacterial and anti-mite spray according to any one of claims 1-7, characterized in that, The preparation method includes the following steps: S1. Add deionized water to a mixing tank and heat to 30-35℃. Add tetrasodium diacetate of glutamic acid while stirring, and stir for 3-5 minutes until it is completely dissolved. S2. Mix dichlorobenzyl alcohol, fragrance, glycerin, PEG-40 hydrogenated castor oil, and ethylhexyl glycerin to form a transparent solution; S3. Add laurylamine dipropylene diamine, soybean ethyl sulfate morpholine, and dodecyl dimethyl benzyl ammonium chloride to the solution obtained in S1, and stir for 10-15 minutes to completely dissolve it. S4. While stirring, add the mixture obtained in S2 to the solution obtained in S3, and stir for 15-25 minutes until it is completely dissolved. S5. While stirring, add lactic acid, persimmon extract and lactobacillus / rye flour fermentation product to the solution obtained in S4, stir for 10-15 minutes, and adjust the pH to slightly acidic. S6. Filter the material through an 800-850 mesh filter, fill it, and you will get a high-efficiency antibacterial and anti-mite spray.

9. The preparation method of the high-efficiency antibacterial and anti-mite spray according to claim 8, characterized in that, The pH of the solution obtained in step S5 is 4.0-7.

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10. The method for preparing the high-efficiency antibacterial and anti-mite spray according to claim 9, characterized in that, The pH of the solution obtained in step S5 is 5.9.

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