Whitening and soothing composition, nano microemulsion containing same and preparation method of nano microemulsion

Abstract

The invention relates to a whitening and soothing composition, a nano microemulsion containing the same and a preparation method of the nano microemulsion. The whitening and soothing composition comprises a litchi fruit extract, a quercus root extract, a malt extract and palmitoyl tripeptide-5. According to the functional composition, the litchi fruit extract, the oak root extract, the malt extract and the palmitoyl tripeptide-5 are creatively combined for use, so that the functional composition has excellent skin whitening and soothing effects, and the four components have an obvious synergistic interaction effect in the aspects of whitening and soothing the skin; the functional composition can be widely applied to cosmetics with related functions. The nano micro-emulsion prepared from the functional composition is subjected to shearing emulsification and then high-pressure micro-jet treatment, so that the prepared nano micro-emulsion is better in stability and better in appearance property, and the nano micro-emulsion can stably play a role in whitening and relieving for a long time.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to a whitening and soothing composition, a nanoemulsion containing the same, and a method for preparing the same. Background Technology

[0002] Whitening products are applied directly to facial skin, requiring not only effective and comprehensive whitening and spot-removing properties, but also gentle comfort to prevent skin allergies and related skin problems. They must also effectively protect against external irritants for more comprehensive and lasting whitening results—qualities often overlooked in existing whitening and spot-removing products. Therefore, whitening products generally face the challenge of balancing safety and efficacy, leading to increased attention to natural and pure skincare concepts and a growing emphasis on the rational combination of whitening functional ingredients in product formulations.

[0003] Skin color differences are primarily determined by the melanin-producing capacity of melanocytes. The quantity, type, and distribution of melanin play a decisive role in skin color. Tyrosinase, dopachrome metaplasia enzyme, DHICA oxidase, and endothelin play significant roles in melanin formation. Melanocytes in the skin are responsible for producing melanin. Ultraviolet radiation can activate melanocytes and increase the activity of tyrosinase. In melanocytes, tyrosine is converted into dopa by tyrosinase. Dopa is further oxidized to form dopaquinone, which then undergoes further oxidation to form melanin. This melanin is transferred to epidermal cells through basal cell division and then excreted through metabolism. When metabolism slows down, melanin accumulates, forming age spots, freckles, and dullness. Besides ultraviolet radiation, other factors such as skin inflammation (inflammatory factors, inflammatory mediators, etc.), stress, exhaust gases, and reactive oxygen species can also stimulate melanocyte activity, leading to excessive melanin production. Therefore, skin whitening often requires the assistance of other treatments, especially soothing agents, which can reduce melanin production by alleviating external irritation to the skin.

[0004] Most conventional whitening cosmetics suffer from the following defects: the selected raw materials are of a single type; the combination of selected raw materials is unreasonable, resulting in poor synergistic effects. Not only do they fail to enhance efficacy, but they may even cancel each other out, weakening the efficacy of each component when it works alone.

[0005] Furthermore, in the preparation of emulsion-type cosmetics, emulsions formed using traditional emulsification techniques carry the risk of insufficient stability. Over time, the emulsion may separate into oil and water phases, most commonly resulting in a liquid-like surface. Moreover, traditional emulsion techniques produce relatively large particles, which can lead to a thicker texture that is difficult for the skin to absorb, affecting the user experience and efficacy. To ensure emulsion stability, traditional emulsification processes often require a larger amount of emulsifier; however, high concentrations of emulsifiers may irritate sensitive skin or affect the efficacy of active ingredients. Summary of the Invention

[0006] In view of the shortcomings of the prior art, the purpose of this invention is to provide a whitening and soothing composition, a nanoemulsion containing the same, and a method for preparing the same.

[0007] To achieve this objective, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a whitening and soothing composition comprising lychee fruit extract, oak root extract, malt extract and palmitoyl tripeptide-5.

[0009] The claimed efficacy composition of this invention creatively combines four components: lychee fruit extract, oak root extract, malt extract, and palmitoyl tripeptide-5, giving it both excellent skin-whitening and skin-soothing effects. The lychee fruit extract is rich in polyphenols such as proanthocyanidins, catechins, rutin, and quercetin. The oak root extract is a natural component extracted from the dried roots of the oak tree (Quercus acutissima), and its main components include various active substances such as saponins. The malt extract is rich in antioxidants such as vitamin E and flavonoids, as well as polysaccharides. The combination of these three specific plant extracts with palmitoyl tripeptide-5 exhibits a significant synergistic effect in whitening and soothing the skin. This efficacy composition can be widely used in cosmetics with related effects.

[0010] Preferably, the whitening and soothing composition comprises, by weight, 0.01-0.3 parts of lychee fruit extract, 0.01-1 parts of oak root extract, 0.01-2.8 parts of malt extract and 0.01-3 parts of palmitoyl tripeptide-5.

[0011] The above-mentioned mass fractions are based on the actual mass of litchi fruit extract, oak root extract, malt extract and palmitoyl tripeptide-5 in commercially available raw materials. In addition to the actual active ingredients, commercially available raw materials may selectively contain any one or at least two of the following: solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents and antioxidants.

[0012] The mass fractions of the lychee fruit extract can be selected from 0.01 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.12 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, etc.; the mass fractions of the oak root extract can be selected from 0.01 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part, etc.; the mass fractions of the malt extract can be selected from 0.01 parts, 0.05 parts, 0.1 parts, etc. The mass fractions of palmitoyl tripeptide-5 can be selected from 0.01 parts, 0.05 parts, 0.1 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.7 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.5 parts, 2.8 parts, 3 parts, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0013] Based on the synergistic effects of lychee fruit extract, oak root extract, malt extract, and palmitoyl tripeptide-5 in whitening and soothing the skin, the effects are even better when they meet the above specific quality ratio.

[0014] The litchi fruit extract, oak root extract, and malt extract selected in the composition of the present invention can be prepared using conventional extraction methods in the art, but the following specific preparation process is preferred.

[0015] Preferably, the litchi fruit extract is prepared by a method comprising the following steps:

[0016] The litchi pulp was pulped, mixed with enzymes for enzymatic hydrolysis, filtered, and the filtrate was concentrated and dried to obtain the litchi fruit extract.

[0017] Preferably, the amount of enzyme used is 0.1-0.5% of the litchi pulp, such as 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, etc.

[0018] Preferably, the enzyme is a combination of pectinase and cellulase.

[0019] Preferably, the mass ratio of pectinase to cellulase is 1:2 to 2:1, such as 1:2, 2:3, 3:4, 1:1, 4:3, 3:2, 2:1, etc.

[0020] Preferably, the enzymatic hydrolysis treatment is carried out at a temperature of 45-55℃, such as 45℃, 47℃, 48℃, 49℃, 50℃, 51℃, 52℃, 53℃, 54℃, 55℃, etc.; and for a time of 1-2 hours, such as 1 hour, 1.1 hours, 1.2 hours, 1.4 hours, 1.5 hours, 1.7 hours, 1.8 hours, 2 hours, etc.

[0021] Preferably, the filtration method includes plate and frame filtration or centrifugal filtration.

[0022] Preferably, the filtrate is concentrated under reduced pressure until the solid content is 20-30%.

[0023] Preferably, the drying method includes spray drying.

[0024] Other specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0025] Preferably, the oak root extract is prepared by a method comprising the following steps:

[0026] The oak root was dried, pulverized, mixed with an ethanol-water solution, and then refluxed for extraction. The extract was purified and eluted through a macroporous adsorption resin column. The eluent was concentrated and dried to obtain the oak root extract.

[0027] Preferably, the drying process is carried out until the moisture content is less than 10%, for example, 9%, 8%, 7%, 6%, 5%, 4%, etc.; the pulverization process is carried out to 20-40 mesh, for example, 20 mesh, 22 mesh, 25 mesh, 28 mesh, 30 mesh, 32 mesh, 35 mesh, 36 mesh, 38 mesh, 40 mesh, etc.

[0028] Preferably, the ethanol concentration of the aqueous ethanol solution is 70-80%, such as 70%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 80%, etc.

[0029] Preferably, the amount of the ethanol aqueous solution used is 8-10 times the mass of the oak root, for example, 8 times, 8.5 times, 9 times, 9.5 times, 10 times, etc.

[0030] Preferably, the reflux extraction is performed 1-3 times, for example, once, twice or three times; each time for 1.5-2 hours, for example, 1.5 hours, 1.6 hours, 1.7 hours, 1.8 hours, 1.9 hours, 2 hours, etc.

[0031] Preferably, the packing material of the macroporous adsorption resin column includes AB-8, D101, HPD100, and HPD300.

[0032] Preferably, the elution method is as follows: first elute with water, then elute with a 50-60% ethanol aqueous solution, and collect the eluent.

[0033] Preferably, the concentration is reduced to a thick paste.

[0034] Preferably, the drying method includes vacuum drying.

[0035] Other specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0036] Preferably, the malt extract is prepared by a method comprising the following steps:

[0037] The malt is roasted and pulverized, then mixed with water, soaked, heated and extracted, the extract is filtered, centrifuged, the clear liquid is concentrated and dried to obtain the malt extract.

[0038] Preferably, the material is pulverized to 20-40 mesh, such as 20 mesh, 22 mesh, 25 mesh, 28 mesh, 30 mesh, 32 mesh, 35 mesh, 36 mesh, 38 mesh, 40 mesh, etc.

[0039] Preferably, the amount of water used is 10-12 times the mass of the malt, for example, 10 times, 10.5 times, 11 times, 11.5 times, 12 times, etc.

[0040] Preferably, the soaking time is 15-45 minutes, such as 15 minutes, 18 minutes, 20 minutes, 22 minutes, 25 minutes, 28 minutes, 30 minutes, 35 minutes, 40 minutes, 45 minutes, etc.

[0041] Preferably, the heating extraction is carried out at boiling point, and the number of times is 1-3, for example, 1, 2 or 3 times; each time is 1-1.5 hours, for example, 1 hour, 1.1 hours, 1.2 hours, 1.3 hours, 1.4 hours, 1.5 hours, etc.

[0042] Preferably, the centrifugation is performed at 5000-6000 rpm for 15-20 minutes.

[0043] Preferably, the extract is concentrated to a relative density of 1.10-1.20.

[0044] Preferably, the drying method includes spray drying or vacuum freeze drying.

[0045] Other specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0046] In a second aspect, the present invention provides the use of the whitening and soothing composition according to the first aspect in the preparation of cosmetics.

[0047] Preferably, the cosmetic includes toner, lotion, cream, mask, serum, or gel.

[0048] Thirdly, the present invention provides a nanoemulsion with whitening and soothing effects, wherein the raw materials for preparing the nanoemulsion include the whitening and soothing composition described in the first aspect, a moisturizer, a skin moisturizer, an emulsifier, a thickener, and water.

[0049] Preferably, the raw materials for preparing the nanoemulsion include, by mass parts, 0.1-8 parts of the whitening and soothing composition described in the first aspect, 1-10 parts of moisturizer, 1-6 parts of emollient, 1-15 parts of emulsifier, 0.01-0.5 parts of thickener, and 60-95 parts of water.

[0050] The whitening and soothing composition can be selected in parts by weight of 0.1, 0.5, 1, 2, 3, 4, 5, 6, 7, or 8; the moisturizer can be selected in parts by weight of 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10; and the emollient can be selected in parts by weight of 1, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, or 6. Other specific values ​​within the above ranges are also acceptable and will not be elaborated upon here.

[0051] Preferably, the raw materials for preparing the nanoemulsion also include any one or a combination of at least two of preservatives, fragrances, or antibacterial agents.

[0052] Preferably, the emulsifier comprises any one or a combination of at least two of polyglycerol-10 stearate, polyglycerol-10 oleate, PPG-13-decyltetradecyl alcohol polyether-24, polysorbate-80, polysorbate-20, or polyglycerol-10 myristate.

[0053] Preferably, the emulsifier is a combination of polyglycerol-10 stearate, polyglycerol-10 oleate, PPG-13-decyltetradecyl alcohol polyether-24, polysorbate-80, polysorbate-20 and polyglycerol-10 myristate.

[0054] Preferably, the mass ratio of polyglycerol-10 stearate, polyglycerol-10 oleate, PPG-13-decyltetradecyl alcohol polyether-24, polysorbate-80, polysorbate-20 and polyglycerol-10 myristate is (1-20):(1-20):(10-50):(5-15):(0.5-5):(3-6.5).

[0055] The specific point values ​​in (1-20) can be 1, 3, 4, 8, 10, 12, 15, 18, 20, etc.; the specific point values ​​in (10-50) can be 10, 15, 20, 25, 30, 35, 40, 45, 50, etc.; the specific point values ​​in (5-15) can be 5, 6, 8, 10, 11, 12, 13, 14, 15, etc.; the specific point values ​​in (0.5-5) can be 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, etc.; the specific point values ​​in (3-6.5) can be 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, etc.; other specific point values ​​within the above range can be selected, and will not be elaborated here.

[0056] Preferably, the emollient comprises any one or a combination of at least two of the following: squalane, rice bran oil, olive fruit oil, meadowfoam seed oil, shea butter, sweet almond oil, or grape seed oil.

[0057] Preferably, the thickener comprises any one or a combination of at least two of the following: sodium polyacrylate, carbomer, xanthan gum, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, sorbitan isostearate, hydroxyethyl cellulose, polyacrylate crosspolymer-6, and acrylic (ester) copolymers.

[0058] Preferably, the humectant includes any one or a combination of at least two of the following: betaine, trehalose, sodium hyaluronate, erythritol, sodium hyaluronate, hydrolyzed sodium hyaluronate, glycerin, butylene glycol, soluble proteoglycans, glyceryl polyether-26, and polyglycerol-10.

[0059] Preferably, the preservative includes any one or a combination of at least two of phenoxyethanol, ethylhexylglycerin, phenoxyethanol, methylparaben, propylene glycol, or ethylparaben.

[0060] Preferably, the antibacterial agent comprises any one or a combination of at least two of 1,3-propanediol, caprylyl glycol, ethylhexylglycerin, capryloyl hydroxamic acid, or 1,2-hexanediol.

[0061] Fourthly, the present invention provides a method for preparing nanoemulsions according to the third aspect, the method comprising the following steps:

[0062] (1) The emollient is mixed with water and heated and homogenized. The homogenized product is mixed with emulsifier, thickener and moisturizer and shear emulsification treatment is performed. After cooling, it is mixed with whitening and soothing composition and homogenized to obtain primary emulsion.

[0063] (2) The primary emulsion is subjected to high-pressure microjet treatment to obtain the nano-microemulsion.

[0064] This invention creatively employs a method of first performing shear emulsification followed by high-pressure microjet treatment, resulting in nanoemulsions with better stability and superior appearance, and which can exert their whitening and soothing effects stably for a long time.

[0065] Preferably, the heating is carried out to 60-70℃, such as 60℃, 62℃, 65℃, 66℃, 68℃, 70℃, etc.; and the cooling is carried out to 40-50℃, such as 40℃, 42℃, 43℃, 44℃, 45℃, 48℃, 50℃, etc.

[0066] Preferably, the homogenization is performed at 300-700 rpm (e.g., 300 rpm, 400 rpm, 500 rpm, 600 rpm, 700 rpm, etc.) for 5-15 minutes (e.g., 5 minutes, 7 minutes, 8 minutes, 9 minutes, 10 minutes, 12 minutes, 13 minutes, 15 minutes, etc.).

[0067] Preferably, the shear emulsification process is performed at 8000-12000 rpm (e.g., 8000 rpm, 9000 rpm, 10000 rpm, 11000 rpm, 12000 rpm, etc.) for 5-15 minutes (e.g., 5 minutes, 7 minutes, 8 minutes, 9 minutes, 10 minutes, 12 minutes, 13 minutes, 15 minutes, etc.).

[0068] Preferably, the pressure of the high-pressure microjet treatment is 4000-7000 psi, such as 4000 psi, 5000 psi, 6000 psi, 7000 psi, etc.; and the treatment is cyclically processed 1-4 times, such as once, twice, three times or four times.

[0069] Other specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0070] Compared with the prior art, the present invention has the following beneficial effects:

[0071] The efficacy composition claimed in this invention creatively combines four components—lychee fruit extract, oak root extract, malt extract, and palmitoyl tripeptide-5—to achieve both excellent skin whitening and skin soothing effects. The four components exhibit a significant synergistic effect in whitening and soothing the skin, and this efficacy composition can be widely used in cosmetics with related effects.

[0072] The nanoemulsion prepared with this functional composition also has excellent whitening and soothing effects. Furthermore, the innovative method of first performing shear emulsification and then high-pressure microjet treatment results in better stability and superior appearance of the prepared nanoemulsion. Moreover, it can stably exert its whitening and soothing effects for a long time. Detailed Implementation

[0073] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0074] The pectinase mentioned below is a product with the brand name "pectinase" purchased from Taian Xindeli Bioengineering Co., Ltd.; the cellulase is a product with the brand name "cellulase" purchased from Taian Xindeli Bioengineering Co., Ltd.; and the sodium polyacrylate is a product with the brand name "sodium polyacrylate" purchased from Wuhan Lanabai Pharmaceutical Chemical Co., Ltd.

[0075] Preparation Example 1-1

[0076] This preparation example provides a litchi fruit extract, which is prepared by the following method:

[0077] The litchi pulp was mixed with twice its weight of water and then pulped. Then, pectinase and cellulase were added in a 1:1 mass ratio (the total amount of enzymes was 0.3% of the litchi pulp) for enzymatic hydrolysis. The hydrolysis temperature was 50℃ and the time was 1.5h. The extract was centrifuged at 4000rpm for 5min. The supernatant was concentrated under vacuum (0.08MPa, 55℃) until the solid content was 25%. The concentrate was spray-dried to obtain litchi fruit extract powder.

[0078] Preparation Examples 1-2

[0079] This preparation example provides a litchi fruit extract, which is prepared by the following method:

[0080] The litchi pulp was mixed with twice its weight of water and then pulped. Then, pectinase and cellulase were added in a mass ratio of 1:2 (the total amount of enzymes was 0.4% of the litchi pulp) for enzymatic hydrolysis. The hydrolysis temperature was 55℃ and the time was 1 hour. The extract was centrifuged at 4000 rpm for 5 minutes. The supernatant was concentrated under vacuum (0.08 MPa, 55℃) until the solid content was 25%. The concentrate was spray-dried to obtain litchi fruit extract powder.

[0081] Preparation Examples 1-3

[0082] This preparation example provides a litchi fruit extract, which is prepared by the following method:

[0083] The litchi pulp was mixed with twice its weight of water and then pulped. Then, pectinase and cellulase were added in a mass ratio of 2:1 (the total amount of enzymes was 0.2% of the litchi pulp) for enzymatic hydrolysis. The hydrolysis temperature was 45℃ and the time was 2h. The extract was centrifuged at 4000rpm for 5min. The supernatant was concentrated under vacuum (0.08MPa, 55℃) until the solid content was 25%. The concentrate was spray-dried to obtain litchi fruit extract powder.

[0084] Preparation Examples 1-4

[0085] This preparation example provides a litchi fruit extract, which is prepared by ethanol reflux extraction:

[0086] The litchi pulp was dried until the moisture content was less than 10%, mixed with 10 times its weight of 75% ethanol aqueous solution, and refluxed twice for 1.5 hours each time. The two extracts were combined, concentrated to a thick paste, and vacuum dried to obtain litchi fruit extract powder.

[0087] Preparation Example 2-1

[0088] This preparation example provides an oak root extract, prepared by the following method:

[0089] The oak roots were dried until the moisture content was less than 10%, pulverized to 40 mesh, and mixed with 10 times their weight of 75% ethanol aqueous solution. The mixture was then refluxed twice for 1.5 hours each time. The two extracts were combined and purified by elution through a D101 macroporous adsorption resin column. The solution was first eluted with water and discarded, and then eluted with 55% ethanol aqueous solution. The ethanol eluent was collected, concentrated to a thick paste, and then vacuum dried to obtain oak root extract powder.

[0090] Preparation Example 2-2

[0091] This preparation example provides an oak root extract, prepared by the following method:

[0092] The oak roots were dried until the moisture content was less than 10%, pulverized to 40 mesh, and mixed with 8 times their weight of 80% ethanol aqueous solution. The mixture was then refluxed and extracted three times, each time for 1 hour. The three extracts were combined and purified by elution through a D101 macroporous adsorption resin column. The solution was first eluted with water and discarded, and then eluted with 60% ethanol aqueous solution. The ethanol eluent was collected, concentrated to a thick paste, and then vacuum dried to obtain oak root extract powder.

[0093] Preparation Examples 2-3

[0094] This preparation example provides an oak root extract, prepared by the following method:

[0095] The oak roots were dried until the moisture content was less than 10%, pulverized to 40 mesh, and mixed with 10 times their weight of 70% ethanol aqueous solution. The mixture was then refluxed twice for 2 hours each time. The two extracts were combined and purified by elution through a D101 macroporous adsorption resin column. The solution was first eluted with water and discarded, and then eluted with 50% ethanol aqueous solution. The ethanol eluent was collected, concentrated to a thick paste, and then vacuum dried to obtain oak root extract powder.

[0096] Preparation Examples 2-4

[0097] This preparation example provides an oak root extract, which is prepared by water extraction:

[0098] The oak root was dried and pulverized to 40 mesh, mixed with 10 times its weight of water, heated to boiling, and boiled twice for 1 hour each time. The two decoctions were combined, filtered, and the filtrate was centrifuged at 5000 rpm for 15 minutes. The supernatant was concentrated under reduced pressure and spray-dried to obtain oak root extract powder.

[0099] Preparation Example 3-1

[0100] This preparation example provides a malt extract, prepared by the following method:

[0101] The malt was roasted and pulverized to 40 mesh, mixed with 10 times its weight of water, soaked at 30°C for 30 minutes, heated to boiling, and boiled twice for 1 hour each time. The two decoctions were combined, filtered, and the filtrate was centrifuged at 5000 rpm for 15 minutes. The supernatant was concentrated under reduced pressure and spray-dried to obtain malt extract powder.

[0102] Preparation Example 3-2

[0103] This preparation example provides a malt extract, prepared by the following method:

[0104] The malt was roasted and pulverized to 40 mesh, mixed with 12 times its weight of water, soaked at 25°C for 45 minutes, heated to boiling, and boiled twice for 1.5 hours each time. The two decoctions were combined, filtered, and the filtrate was centrifuged at 6000 rpm for 10 minutes. The supernatant was concentrated under reduced pressure and spray-dried to obtain malt extract powder.

[0105] Preparation Example 3-3

[0106] This preparation example provides a malt extract, prepared by the following method:

[0107] The malt was roasted and pulverized to 40 mesh, mixed with 12 times its weight of water, soaked at 40℃ for 25 minutes, heated to boiling, and boiled three times for 1 hour each time. The three decoctions were combined, filtered, and the filtrate was centrifuged at 6000 rpm for 10 minutes. The supernatant was concentrated under reduced pressure and spray-dried to obtain malt extract powder.

[0108] Preparation Examples 3-4

[0109] This preparation example provides a malt extract, which is prepared by ethanol reflux extraction:

[0110] The malt was roasted and pulverized to 40 mesh, mixed with 10 times its weight of 75% ethanol aqueous solution, and refluxed twice for 1.5 hours each time. The two extracts were combined, concentrated to a thick paste, and vacuum dried to obtain malt extract powder.

[0111] Example 1

[0112] This embodiment provides a whitening and soothing composition comprising the following active ingredients in parts by weight: 0.2 parts of litchi fruit extract from Preparation Example 1-1, 0.7 parts of oak root extract from Preparation Example 2-1, 0.9 parts of malt extract from Preparation Example 3-1, and 1.2 parts of palmitoyl tripeptide-5.

[0113] Example 2

[0114] This embodiment provides a whitening and soothing composition comprising the following active ingredients in parts by weight: 0.1 parts of lychee fruit extract from Preparation Examples 1-2, 0.9 parts of oak root extract from Preparation Examples 2-2, 0.5 parts of malt extract from Preparation Example 3-2, and 1.8 parts of palmitoyl tripeptide-5.

[0115] Example 3

[0116] This embodiment provides a whitening and soothing composition comprising the following active ingredients in parts by weight: 0.3 parts of lychee fruit extract from Preparation Examples 1-3, 0.1 parts of oak root extract from Preparation Examples 2-3, 2.5 parts of malt extract from Preparation Examples 3-3, and 0.9 parts of palmitoyl tripeptide-5.

[0117] Example 4

[0118] This embodiment provides a whitening and soothing composition, the only difference between which is the lychee fruit extract of Preparation Example 1-1 replaced with the lychee fruit extract of Preparation Example 1-4, while the other components and contents remain unchanged.

[0119] Example 5

[0120] This embodiment provides a whitening and soothing composition, the only difference between which is that the oak root extract of Preparation Example 2-1 is replaced with the oak root extract of Preparation Example 2-4 in equal proportions, while the other components and contents remain unchanged.

[0121] Example 6

[0122] This embodiment provides a whitening and soothing composition, the only difference between which is that the malt extract of Preparation Example 3-1 is replaced with the malt extract of Preparation Example 3-4 in equal proportions, while the other components and contents remain unchanged.

[0123] Comparative Example 1

[0124] This embodiment provides a composition whose components differ from those of Example 1 only in that the litchi fruit extract is omitted. The reduction is proportionally allocated to the proportions of oak root extract, malt extract, and palmitoyl tripeptide-5, while the others remain unchanged.

[0125] Comparative Example 2

[0126] This embodiment provides a composition whose components differ from those of Example 1 only in that it lacks oak root extract. The reduction is proportionally allocated to the proportions of litchi fruit extract, malt extract, and palmitoyl tripeptide-5, while the others remain unchanged.

[0127] Comparative Example 3

[0128] This embodiment provides a composition whose components differ from those of Example 1 only in that malt extract is omitted. The reduction is proportionally allocated to the proportions of litchi fruit extract, oak root extract, and palmitoyl tripeptide-5, while the others remain unchanged.

[0129] Comparative Example 4

[0130] This embodiment provides a composition whose components differ from those of Example 1 only in the absence of palmitoyl tripeptide-5. The reduction in amount is proportionally distributed across the proportions of litchi fruit extract, oak root extract, and malt extract, while the others remain unchanged.

[0131] Application Example 1

[0132] This application example provides a nanoemulsion with whitening and soothing effects, the raw material formula of which is shown in the table below:

[0133]

[0134] Its preparation method is as follows:

[0135] (1) After mixing the emollient with water, heat it to 65°C and stir it at 500 rpm for 15 min using a homogenizer. After mixing the homogenized product with the emulsifier, thickener and moisturizer, perform shear emulsification using a high shear emulsifier (10000 rpm, 5 min). After cooling to 45°C, mix it with the whitening and soothing composition and stir it at 500 rpm for 10 min using a homogenizer to obtain the primary emulsion.

[0136] (2) The primary emulsion is fed into the feed port of the microjet device and subjected to high-pressure microjet treatment. The homogenization pressure is set to 1000 bar, and the process is repeated 4 times. The emulsion is then cooled to 25°C to obtain the nano-microemulsion.

[0137] Application Example 2-6

[0138] This application example provides five nanoemulsions with whitening and soothing effects. The only difference between the raw material formulation and application example 1 is that the whitening and soothing composition of example 1 is replaced in equal amounts with the whitening and soothing compositions of examples 2-6. Other components and their contents remain unchanged.

[0139] Application Example 7

[0140] This application example provides a nanoemulsion with whitening and soothing effects. The difference between its raw material formulation and that of Application Example 1 is only in the formulation of the emulsifier: polyglycerol-10 stearate and polyglycerol-10 oleate are missing, and their reduced amounts are proportionally allocated to the content of other emulsifiers.

[0141] Application Example 8

[0142] This application example provides a nano-microemulsion with whitening and soothing effects. The only difference between its raw material formulation and that of Application Example 1 is the emulsifier formulation: PPG-13-decyltetradecyl alcohol polyether-24 is missing, and its reduced amount is proportionally allocated to the content of other emulsifiers.

[0143] Application Example 9

[0144] This application example provides a nano-microemulsion with whitening and soothing effects. The difference between its raw material formulation and that of Application Example 1 is only in the formulation of the emulsifier: polysorbate-80 and polysorbate-20 are missing, and their reduced amounts are proportionally allocated to the content of other emulsifiers.

[0145] Application Example 10

[0146] This application example provides a nanoemulsion with whitening and soothing effects. The difference between its raw material formulation and that of Application Example 1 is only in the formulation of the emulsifier: polyglycerol-10 myristate is missing, and its reduced amount is proportionally allocated to the content of other emulsifiers.

[0147] Compare and contrast examples 1-4

[0148] This comparative application example provides four nanoemulsions with whitening and soothing effects. The only difference between these and Application Example 1 is that the whitening and soothing composition of Example 1 is replaced in equal amounts with the compositions of Comparative Examples 1-4. Other components and their contents remain unchanged.

[0149] Comparative Application Example 5

[0150] This comparative application example provides a nanoemulsion with whitening and soothing effects. Its raw material formulation is the same as that of Application Example 1, and its preparation method is as follows:

[0151] The emollient was mixed with water and heated to 65°C. The mixture was then stirred at 500 rpm for 15 min using a homogenizer. The homogenized product was then mixed with emulsifier, thickener, and moisturizer and subjected to shear emulsification using a high-shear emulsifier (10,000 rpm, 20 min). After cooling to 45°C, the mixture was mixed with the whitening and soothing composition and stirred at 500 rpm for 30 min using a homogenizer. The mixture was then cooled to 25°C to obtain the nanoemulsion.

[0152] Test Example 1

[0153] Particle size testing:

[0154] For the nanoemulsions corresponding to examples 1-3, particle size was measured after the initial emulsion was prepared and after the second, third, and fourth high-pressure microjets were processed. The volume average particle size of the dispersed particles was determined using a DelsaMax CORE nanoparticle size analyzer and conventional methods.

[0155] Specifically: Place the instrument on a fixed platform, turn on the power, ensure the instrument and computer are properly connected, and allow the instrument to warm up for 15 minutes before starting the experiment. The measurement indicator light will be green. During the experiment, press down the sample chamber door, insert the cleaned sample cell to the bottom of the sample slot, ensuring the sample cell surface is dry. Close the chamber lid by hand, and retain the sample in the instrument for approximately 30 seconds. The sample can then be tested using software. Record 10 sets of data for each test and take the average. The results are shown in Table 1.

[0156] Table 1

[0157] sample Application Example 1 Application Example 2 Application Example 3 colostrum 2.382 2.466 2.554 Second microfluidic treatment 0.372 0.355 0.366 Microfluidic treatment for the third time 0.264 0.241 0.233 4th microfluidic treatment 0.207 0.198 0.218

[0158] As shown in Table 1, the present invention employs a method of first performing shear emulsification and then high-pressure microfluidic treatment, which results in smaller particle size of the prepared nanoemulsion, providing a basis for improved stability. Furthermore, the smaller particle size is more conducive to skin absorption, which can further enhance the whitening and soothing effects of the product.

[0159] Test Example 2

[0160] Stability test:

[0161] The nanoemulsions prepared in Application Examples 1-10 and Comparative Application Examples 1-5 were placed in sealed containers and stored at 25°C (room temperature), 4°C in a refrigerator, and 45°C in an incubator for 3 months, respectively. The appearance of the samples was then observed to determine their stability. The results are shown in Table 2.

[0162] Table 2

[0163]

[0164]

[0165] As shown in Table 2, the present invention employs a method of first performing shear emulsification followed by high-pressure microjets, and selects emulsifiers with specific formulations, resulting in nanoemulsions that exhibit excellent stability at room temperature, low temperature, and high temperature.

[0166] Test Example 3

[0167] Whitening efficacy test:

[0168] Test Method: 150 volunteers (aged 20-50, male and female) were divided into groups, with 10 volunteers participating for each sample. Under stable indoor conditions (temperature 24-27℃, relative humidity 40-50%), the initial ITA value of each group's facial skin was measured using a multifunctional skin analyzer (model: MPA580) manufactured by CourageKhazaka, Germany. Subjects used equal amounts of the products prepared for Examples 1-10 and Control Examples 1-5, applying them twice daily after cleansing, morning and evening, maintaining a consistent application amount, for 4 weeks. The ITA value of each group's facial skin was measured on day 0 (D0) and day 28 (D28) (average value was taken). The average ITA change rate was calculated using the formula: ITA change rate (%) = (post-use data - pre-use data) / pre-use data × 100% to evaluate the whitening effect of the test product. A higher average ITA change rate indicated a better skin tone brightening effect. The results are shown in Table 3.

[0169] Table 3

[0170] Group Average rate of change of ITA (%) Application Example 1 12.35 Application Example 2 10.98 Application Example 3 11.37 Application Example 4 8.61 Application Example 5 7.69 Application Example 6 8.32 Application Example 7 9.62 Application Example 8 10.54 Application Example 9 10.06 Application Example 10 9.82 Comparative Application Example 1 5.69 Comparative Application Example 2 4.63 Comparative Application Example 3 6.04 Comparative Application Example 4 7.32 Comparative Application Example 5 9.31

[0171] As shown in Table 3, this invention combines four components—lychee fruit extract, oak root extract, malt extract, and palmitoyl tripeptide-5—as the active ingredients in a nanoemulsion. The preparation method employs a combination of shear emulsification followed by high-pressure microfluidic treatment, resulting in a nanoemulsion with excellent whitening effects. Furthermore, the emulsifier formulation in the nanoemulsion affects the product's stability, thus influencing its whitening effect to some extent. Additionally, the preparation processes of the lychee fruit extract, oak root extract, and malt extract also affect the product's whitening effect to some degree.

[0172] Test Example 4

[0173] Soothing efficacy test:

[0174] Testing Method: 150 volunteers (aged 20-50, male or female, from the same batch as test case 3) were divided into groups, with 10 volunteers participating for each sample. The inner forearm was the test site. Both forearms were normal skin without skin lesions, redness, flaking, or other discomfort. Subjects were tested in a stable indoor environment with a temperature of 24-27℃ and a relative humidity of 40-50%. A skin color meter was used. The CL400 was used to measure the skin color on the inner forearm of the subjects. The experimenter peeled off adhesive tape 15 times in each of the two test areas, then measured the skin color on the inner forearm. Immediately afterward, nanoemulsions prepared in Application Examples 1-10 and Comparative Examples 1-5 were applied to the test areas, and the skin color on the inner forearm was measured immediately. The control areas served as blank controls. Data changes were recorded immediately after the 15 tape peels and 8 hours after sample application. A higher a* value indicates a redder skin color. The results are shown in Table 4.

[0175] Table 4

[0176]

[0177]

[0178] As shown in Table 4, this invention combines four components—lychee fruit extract, oak root extract, malt extract, and palmitoyl tripeptide-5—as the active ingredients in the nanoemulsion. The preparation method employs a combination of shear emulsification followed by high-pressure microfluidic treatment, resulting in a nanoemulsion with excellent soothing effects (a* value reduction). Furthermore, the emulsifier formulation in the nanoemulsion affects the product's stability, thus influencing its soothing effect to some extent. Additionally, the preparation processes of the lychee fruit extract, oak root extract, and malt extract also affect the product's soothing effect to some degree.

[0179] The applicant declares that the technical solution of this invention is illustrated by the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of this invention.

[0180] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0181] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A whitening and soothing composition, characterized in that, The whitening and soothing composition includes lychee fruit extract, oak root extract, malt extract, and palmitoyl tripeptide-5.

2. The whitening and soothing composition according to claim 1, characterized in that, The whitening and soothing composition comprises, by weight parts, 0.01-0.3 parts of lychee fruit extract, 0.01-1 parts of oak root extract, 0.01-2.8 parts of malt extract and 0.01-3 parts of palmitoyl tripeptide-5.

3. The whitening and soothing composition according to claim 1 or 2, characterized in that, The litchi fruit extract is prepared by a method comprising the following steps: The litchi pulp was pulped, mixed with enzymes for enzymatic hydrolysis, filtered, and the filtrate was concentrated and dried to obtain the litchi fruit extract. Preferably, the amount of enzyme used is 0.1-0.5% of the litchi pulp; Preferably, the enzyme is a combination of pectinase and cellulase; Preferably, the mass ratio of pectinase to cellulase is 1:2-2:1; Preferably, the enzymatic hydrolysis treatment is performed at a temperature of 45-55°C for 1-2 hours.

4. The whitening and soothing composition according to claim 1 or 2, characterized in that, The oak root extract is prepared by a method comprising the following steps: The oak root was dried, pulverized, mixed with an ethanol-water solution, and then extracted by reflux. The extract was purified and eluted by passing it through a macroporous adsorption resin column. The eluent was concentrated and dried to obtain the oak root extract. Preferably, the drying process is carried out until the moisture content is less than 10%; the pulverization process is carried out to a mesh size of 20-40. Preferably, the ethanol concentration of the aqueous ethanol solution is 70-80%; Preferably, the amount of the ethanol aqueous solution used is 8-10 times the mass of the oak root; Preferably, the reflux extraction is performed 1-3 times, each time for 1.5-2 hours.

5. The whitening and soothing composition according to claim 1 or 2, characterized in that, The malt extract is prepared by a method comprising the following steps: The malt was roasted and pulverized, then mixed with water, soaked, heated and extracted, the extract was filtered, centrifuged, the clear liquid was concentrated and dried to obtain the malt extract. Preferably, the material is pulverized to 20-40 mesh; Preferably, the amount of water used is 10-12 times the mass of the malt; Preferably, the soaking time is 15-45 minutes; Preferably, the heating extraction is carried out at boiling point, 1-3 times, each time for 1-1.5 hours.

6. The use of the whitening and soothing composition according to any one of claims 1-5 in the preparation of cosmetics.

7. A nanoemulsion with whitening and soothing effects, characterized in that, The raw materials for preparing the nanoemulsion include the whitening and soothing composition according to any one of claims 1-5, a moisturizer, a skin-nourishing agent, an emulsifier, a thickener, and water.

8. The nanoemulsion according to claim 7, characterized in that, The raw materials for preparing the nanoemulsion include, by mass parts, 0.1-8 parts of the whitening and soothing composition as described in any one of claims 1-5, 1-10 parts of moisturizer, 1-6 parts of emulsifier, 1-15 parts of thickener, and 60-95 parts of water; Preferably, the raw materials for preparing the nanoemulsion also include any one or a combination of at least two of the following: preservatives, fragrances, or antibacterial agents; Preferably, the emulsifier comprises any one or a combination of at least two of polyglycerol-10 stearate, polyglycerol-10 oleate, PPG-13-decyltetradecyl alcohol polyether-24, polysorbate-80, polysorbate-20 or polyglycerol-10 myristate; Preferably, the emulsifier is a combination of polyglycerol-10 stearate, polyglycerol-10 oleate, PPG-13-decyltetradecyl alcohol polyether-24, polysorbate-80, polysorbate-20 and polyglycerol-10 myristate; Preferably, the mass ratio of polyglycerol-10 stearate, polyglycerol-10 oleate, PPG-13-decyltetradecyl alcohol polyether-24, polysorbate-80, polysorbate-20 and polyglycerol-10 myristate is (1-20):(1-20):(10-50):(5-15):(0.5-5):(3-6.5); Preferably, the emollient comprises any one or a combination of at least two of the following: squalane, rice bran oil, olive fruit oil, meadowfoam seed oil, shea butter, sweet almond oil, or grape seed oil. Preferably, the thickener comprises any one or a combination of at least two of the following: sodium polyacrylate, carbomer, xanthan gum, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, sorbitan isostearate, hydroxyethyl cellulose, polyacrylate crosspolymer-6, and acrylic (ester) copolymers. Preferably, the humectant includes any one or a combination of at least two of the following: betaine, trehalose, sodium hyaluronate, erythritol, sodium hyaluronate, hydrolyzed sodium hyaluronate, glycerin, butylene glycol, soluble proteoglycans, glyceryl polyether-26, and polyglycerol-10.

9. The method for preparing nanoemulsions according to claim 7 or 8, characterized in that, The preparation method includes the following steps: (1) The emollient is mixed with water and heated and homogenized. The homogenized product is mixed with emulsifier, thickener and moisturizer and shear emulsification treatment is performed. After cooling, it is mixed with whitening and soothing composition and homogenized to obtain primary emulsion. (2) The primary emulsion is subjected to high-pressure microjet treatment to obtain the nano-microemulsion.

10. The method for preparing nanoemulsions according to claim 9, characterized in that, The temperature is then raised to 60-70℃ and cooled to 40-50℃. Preferably, the homogenization is performed at 300-700 rpm for 5-15 minutes; Preferably, the shear emulsification process is performed at 8000-12000 rpm for 5-15 minutes; Preferably, the pressure of the high-pressure microjet treatment is 4000-7000 psi, and the treatment is repeated 1-4 times.

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