Edible fungus polysaccharide health-care food capable of resisting fatigue and improving immunity

Abstract

The invention is suitable for the technical field of health-care food, and provides an anti-fatigue immunity-improving edible mushroom polysaccharide health-care food, which comprises the following raw materials: composite edible mushroom powder, a eucommia ulmoides extract, a kelp extract, a herba lycopi extract, a radix astragali seu hedysari extract, and an enzyme complex microbial inoculant. The composite edible fungus powder comprises armillaria luteo-virens powder, sparassis crispa powder, tremellodon gelatinosum powder and bamboo fungus powder.The edible fungus polysaccharide health-care food capable of resisting fatigue and improving immunity, provided by the invention, has the characteristics of being natural and safe by adding the composite edible fungus powder, the eucommia ulmoides extract, the kelp extract, the herba lycopi extract, the astragalus membranaceus extract and the enzyme complex microbial inoculants; rich edible fungus polysaccharide, plant polysaccharide, antioxidant substances and the like are contained and are cooperated, so that the health-care food has good effects of resisting fatigue and improving immunity.

Description

Technical Field

[0001] This invention belongs to the field of health food technology, and in particular relates to an edible fungus polysaccharide health food that combats fatigue and enhances immunity. Background Technology

[0002] Fatigue is a complex physiological and biochemical process in the body, and it is a normal physiological phenomenon that inevitably occurs when a person's mental or physical strength reaches a certain stage. The occurrence of fatigue can lead to endocrine disorders, decreased immunity, and even organic lesions, becoming an important factor threatening human health. When the body's immunity is low, it reduces the body's ability to fight infection, its ability to recognize and eliminate aging tissue cells, and its ability to kill and eliminate abnormal mutated cells in the body, resulting in a decline in the body's disease resistance and self-healing ability, leaving the body in a state of sub-health for a long time. Edible fungi are large fungi that can be consumed by humans. They contain bioactive substances such as high-molecular-weight polysaccharides, β-glucan and RNA complexes, natural organic germanium, nucleic acid degradation products, cAMP and triterpenoids, which have important utilization value for maintaining human health. This application aims to provide an edible fungi health food with anti-fatigue and immune-enhancing effects, which has significant market value and social significance. Summary of the Invention

[0003] This invention provides an edible fungus polysaccharide health food that combats fatigue and enhances immunity, aiming to solve the aforementioned problems.

[0004] This invention is achieved as follows: an edible fungus polysaccharide health food for combating fatigue and enhancing immunity, comprising the following raw materials in parts by weight: 80-100 parts of compound edible fungus powder, 10-20 parts of Eucommia ulmoides extract, 15-25 parts of kelp extract, 10-30 parts of Lycopus lucidus extract, 5-40 parts of Astragalus membranaceus extract, and 0.1-0.5 parts of enzyme compound microbial agent. The compound edible fungus powder includes Armillaria mellea powder, Hydrangea macrophylla powder, Pteris vittata powder, and Dictyophora indica powder, with a mass ratio of 2-5:1-3:1-2:1.

[0005] Preferably, the raw materials include the following by weight: 85-95 parts of compound edible fungus powder, 12-18 parts of Eucommia ulmoides extract, 18-22 parts of kelp extract, 15-25 parts of Eupatorium fortunei extract, 15-30 parts of Astragalus membranaceus extract, and 0.2-0.4 parts of enzyme compound microbial agent. The compound edible fungus powder includes Armillaria mellea powder, Hydrangea macrophylla powder, Pteris vittata powder, and Dictyophora indica powder, with a mass ratio of 2.5-4.5:1.5-2.5:1.2-1.8:1.

[0006] Preferably, the raw materials include the following by weight: 90 parts of compound edible fungus powder, 15 parts of Eucommia ulmoides extract, 20 parts of kelp extract, 20 parts of Lycopus lucidus extract, 22.5 parts of Astragalus membranaceus extract, and 0.3 parts of enzyme compound microbial agent. The compound edible fungus powder includes Armillaria mellea powder, Hydrangea macrophylla powder, Pteris vittata powder, and Dictyophora indica powder, with a mass ratio of 3.5:2:1.5:1.

[0007] Preferably, the preparation method of the compound edible fungus powder is as follows: Weigh each edible fungus according to the ratio, pulverize the compound edible fungus to a particle size of less than 0.5 mm to obtain pulverized material, and treat it with microwave irradiation at a power of 280-300W for 60-100s during the pulverization process. Add the pulverized material and 15-20 times the weight of water to a container, and then add 1-2 times the weight of carbon nanotubes to the pulverized material. Install positive and negative electrodes on both sides of the container, and stir while applying electricity for 5-10 minutes to obtain a mixture. The voltage of the electricity is 20-40V and the current is 1-5A. Heat the mixture to 50-60℃, and then add 0.1-0.3% of cellulase by weight of the pulverized material for enzymatic hydrolysis for 20-30 minutes. Adjust the temperature to 80-90℃ and maintain it for 2-5 minutes. Then adjust the temperature to 45-55℃, and then add 0.1-0.3% of flavor protease by weight of the pulverized material for enzymatic hydrolysis for 10-20 minutes. Centrifuge to obtain the supernatant, and spray dry the supernatant to obtain the compound edible fungus powder.

[0008] The combination of crushing force and short-term irradiation promotes molecular movement on the surface of edible fungi, loosening the cell wall structure. Then, carbon nanotubes are added, and electricity is applied while stirring. The hollow tubular structure of the carbon nanotubes can mechanically break down the cell wall, and the electricity intensifies the movement of the carbon nanotubes. When the current passes through, it causes the carbon atoms to vibrate under the influence of the electric field, resulting in energy level transitions of electrons around the atomic nuclei and the release of energy in the form of far-infrared rays, forming a uniformly heated planar heating body, which improves the cell wall breakage rate and thus improves the extraction efficiency.

[0009] Preferably, the preparation method of the Eucommia ulmoides extract is as follows: take Eucommia ulmoides, add 10-20 times the weight of deionized water, extract 3 times at 90-100℃, each extraction time is 2-3 hours, filter the extract, combine, concentrate to the solid content of 5-10%, and obtain Eucommia ulmoides extract concentrate. Dry the Eucommia ulmoides extract concentrate to obtain Eucommia ulmoides extract.

[0010] Preferably, the kelp extract is prepared as follows: kelp is taken and added to 10-15 times its weight of deionized water. High-pressure pulse extraction is performed for 30-50 minutes under the conditions of an electric field strength of 10-20 kV / cm, a pulse time of 500-700 μs, and a pulse frequency of 200-400 Hz. After filtration, the extract is spray-dried to obtain the kelp extract.

[0011] Preferably, the extract of *Eupatorium fortunei* is obtained by reflux extraction with ethanol at a mass percentage concentration of 60-80%, the material-to-liquid ratio is 1:10-15, the extraction temperature is 75-85℃, the extraction is performed twice, and the extraction time is 70-100 min. After extraction, the extract is concentrated to a thick paste with a relative density of 1.1-1.2, and finally spray-dried to obtain the extract of *Eupatorium fortunei*.

[0012] Preferably, the preparation method of the Astragalus extract is as follows: take Astragalus, add 8-12 times the weight of deionized water, extract 2-3 times at a temperature of 40-50℃ and a power of 150-250W, each extraction time is 30-50min, concentrate the extracted extract to a thick paste with a relative density of 1.1-1.2, and finally spray dry to obtain the Astragalus extract.

[0013] Preferably, the enzyme compound microbial agent is a mixture of yeast, acetic acid bacteria, and lactic acid bacteria; the weight ratio of yeast, lactic acid bacteria, and acetic acid bacteria is 1:0.5-1.5:1-2, and the colony count of each of the yeast, lactic acid bacteria, and acetic acid bacteria is 10. 8 -10 10 cfu / g.

[0014] The present invention also provides a method for preparing the above-mentioned edible fungi polysaccharide health food for anti-fatigue and immune enhancement, comprising the following steps: Prepare all raw materials according to the specified proportions; After mixing all the ingredients evenly, sterilize them using microwave. The mixture after microwave sterilization is packaged to obtain health food.

[0015] Compared with the prior art, the embodiments of this application have the following main advantages: The edible fungi polysaccharide health food for combating fatigue and enhancing immunity provided by this invention incorporates compound edible fungi powder, Eucommia ulmoides extract, kelp extract, Lycopus lucidus extract, Astragalus membranaceus extract, and enzyme compound microbial agent. It is natural and safe, and contains abundant edible fungi polysaccharides, plant polysaccharides, antioxidants, etc., which work synergistically to have a good effect on combating fatigue and enhancing immunity. Attached Figure Description

[0016] Figure 1 This is a flowchart of a method for preparing an edible fungus polysaccharide health food that combats fatigue and enhances immunity, provided by the present invention. Detailed Implementation

[0017] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used herein in the specification of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having," and any variations thereof, in the specification, claims, and foregoing drawings of this application are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the specification, claims, or foregoing drawings of this application are used to distinguish different objects, not to describe a particular order.

[0018] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0019] Example 1 This invention provides an edible fungus polysaccharide health food for combating fatigue and enhancing immunity, comprising the following raw materials in parts by weight: 80 parts of compound edible fungus powder, 10 parts of Eucommia ulmoides extract, 15 parts of kelp extract, 10 parts of Lycopus lucidus extract, 5 parts of Astragalus membranaceus extract, and 0.1 parts of enzyme compound microbial agent. The compound edible fungus powder includes Armillaria mellea powder, Hydrangea macrophylla powder, Pteris vittata powder, and Dictyophora indicum powder, in a mass ratio of 2:1:1:1. The preparation method of the edible fungus polysaccharide health food for combating fatigue and enhancing immunity is as follows: Figure 1 As shown, it includes the following steps: Prepare all raw materials according to the specified proportions; After mixing all the ingredients evenly, sterilize them using microwave. The mixture after microwave sterilization is packaged to obtain health food.

[0020] The preparation method of the compound edible fungus powder is as follows: Weigh each edible fungus according to the ratio, crush the compound edible fungus to a particle size of less than 0.5 mm to obtain pulverized material, and treat it with microwave irradiation at a power of 280W for 60s during the crushing process. Add the pulverized material and 15 times the weight of water to a container, and then add carbon nanotubes with a weight of 1 times the weight of the pulverized material. Install positive and negative electrodes on both sides of the container, and stir while applying electricity for 5 minutes to obtain a mixture. The voltage of the electricity is 20V and the current is 1A. Heat the mixture to 50℃, and then add 0.1% of cellulase by weight of the pulverized material for enzymatic hydrolysis for 20 minutes. Adjust the temperature to 80℃ and hold for 2 minutes, then adjust the temperature to 45℃, and then add 0.1% of flavor protease by weight of the pulverized material for enzymatic hydrolysis for 10 minutes. Centrifuge to obtain the supernatant, and spray dry the supernatant to obtain the compound edible fungus powder.

[0021] In this embodiment, the preparation method of the Eucommia ulmoides extract is as follows: Eucommia ulmoides is taken, 10 times the weight of deionized water is added, and it is extracted 3 times at 90°C for 2 hours each time. The extract is filtered, combined, and concentrated to a solid content of 5% to obtain Eucommia ulmoides extract concentrate. The Eucommia ulmoides extract concentrate is dried to obtain Eucommia ulmoides extract.

[0022] Preferably, the kelp extract is prepared as follows: kelp is taken and added to 10 times its weight of deionized water, and high-pressure pulse extraction is performed for 30 min under the conditions of field strength of 10 kV / cm, pulse time of 500 μs, and pulse frequency of 200 Hz. After filtration, it is spray-dried to obtain the kelp extract.

[0023] In the specific implementation, the extract of Eupatorium fortunei was obtained by reflux extraction with 60% ethanol by mass percentage. The material-to-liquid ratio during the extraction process was 1:10, the extraction temperature was 75℃, the extraction was performed twice, and the extraction time was 70 min. After extraction, the extract was concentrated to a thick paste with a relative density of 1.1, and finally spray-dried to obtain the extract of Eupatorium fortunei.

[0024] In this embodiment, the preparation method of the Astragalus extract is as follows: Astragalus is taken, and 8 times its weight of deionized water is added. The mixture is extracted twice at a temperature of 40°C and a power of 150W, with each extraction lasting 30 minutes. The extracted extract is concentrated to a thick paste with a relative density of 1.1 and then spray-dried to obtain the Astragalus extract.

[0025] In this embodiment, the enzyme compound microbial agent is a mixture of yeast, acetic acid bacteria, and lactic acid bacteria; the weight ratio of yeast, lactic acid bacteria, and acetic acid bacteria is 1:0.5:1, and the colony count of each of the yeast, lactic acid bacteria, and acetic acid bacteria is 10. 8 cfu / g.

[0026] Example 2 This invention provides an edible fungus polysaccharide health food for combating fatigue and enhancing immunity, comprising the following raw materials in parts by weight: 85 parts of compound edible fungus powder, 12 parts of Eucommia ulmoides extract, 18 parts of kelp extract, 15 parts of Lycopus lucidus extract, 15 parts of Astragalus membranaceus extract, and 0.2 parts of enzyme compound microbial agent. The compound edible fungus powder includes Armillaria mellea powder, Hydrangea macrophylla powder, Pleurotus ostreatus powder, and Dictyophora indicum powder, in a mass ratio of 2.5:1.5:1.2:1. The preparation method of the edible fungus polysaccharide health food for combating fatigue and enhancing immunity is as follows: Figure 1 As shown, it includes the following steps: Prepare all raw materials according to the specified proportions; After mixing all the ingredients evenly, sterilize them using microwave. The mixture after microwave sterilization is packaged to obtain health food.

[0027] The preparation method of the compound edible fungus powder is as follows: Weigh each edible fungus according to the ratio, crush the compound edible fungus to a particle size of less than 0.5 mm to obtain pulverized material, and treat it with microwave irradiation at a power of 280W for 60s during the crushing process. Add the pulverized material and 15 times the weight of water to a container, and then add carbon nanotubes with a weight of 1 times the weight of the pulverized material. Install positive and negative electrodes on both sides of the container, and stir while applying electricity for 5 minutes to obtain a mixture. The voltage of the electricity is 20V and the current is 1A. Heat the mixture to 50℃, and then add 0.13% of cellulase by weight of the pulverized material for enzymatic hydrolysis for 20 minutes. Adjust the temperature to 80℃ and hold for 2 minutes, then adjust the temperature to 45℃, and then add 0.1% of flavor protease by weight of the pulverized material for enzymatic hydrolysis for 10 minutes. Centrifuge to obtain the supernatant, and spray dry the supernatant to obtain the compound edible fungus powder.

[0028] In this embodiment, the preparation method of the Eucommia ulmoides extract is as follows: Eucommia ulmoides is taken, 10 times the weight of deionized water is added, and it is extracted 3 times at 90°C for 2 hours each time. The extract is filtered, combined, and concentrated to a solid content of 5% to obtain Eucommia ulmoides extract concentrate. The Eucommia ulmoides extract concentrate is dried to obtain Eucommia ulmoides extract.

[0029] Preferably, the kelp extract is prepared as follows: kelp is taken and added to 10 times its weight of deionized water, and high-pressure pulse extraction is performed for 30 min under the conditions of field strength of 10 kV / cm, pulse time of 500 μs, and pulse frequency of 200 Hz. After filtration, it is spray-dried to obtain the kelp extract.

[0030] In the specific implementation, the extract of Eupatorium fortunei was obtained by reflux extraction with 60% ethanol by mass percentage. The material-to-liquid ratio during the extraction process was 1:10, the extraction temperature was 75℃, the extraction was performed twice, and the extraction time was 70 min. After extraction, the extract was concentrated to a thick paste with a relative density of 1.1, and finally spray-dried to obtain the extract of Eupatorium fortunei.

[0031] In this embodiment, the preparation method of the Astragalus extract is as follows: Astragalus is taken, and 8 times its weight of deionized water is added. The mixture is extracted twice at a temperature of 40°C and a power of 150W, with each extraction lasting 30 minutes. The extracted extract is concentrated to a thick paste with a relative density of 1.1 and then spray-dried to obtain the Astragalus extract.

[0032] In this embodiment, the enzyme compound microbial agent is a mixture of yeast, acetic acid bacteria, and lactic acid bacteria; the weight ratio of yeast, lactic acid bacteria, and acetic acid bacteria is 1:0.5:1, and the colony count of each of the yeast, lactic acid bacteria, and acetic acid bacteria is 10. 8 cfu / g.

[0033] Example 3 This invention provides an edible fungus polysaccharide health food for combating fatigue and enhancing immunity, comprising the following raw materials in parts by weight: 90 parts of compound edible fungus powder, 15 parts of Eucommia ulmoides extract, 20 parts of kelp extract, 20 parts of Lycopus lucidus extract, 22.5 parts of Astragalus membranaceus extract, and 0.3 parts of enzyme compound microbial agent. The compound edible fungus powder includes Armillaria mellea powder, Hydrangea macrophylla powder, Pleurotus ostreatus powder, and Dictyophora indicum powder, in a mass ratio of 3.5:2:1.5:1. The preparation method of the edible fungus polysaccharide health food for combating fatigue and enhancing immunity is as follows: Figure 1 As shown, it includes the following steps: Prepare all raw materials according to the specified proportions; After mixing all the ingredients evenly, sterilize them using microwave. The mixture after microwave sterilization is packaged to obtain health food.

[0034] The preparation method of the compound edible fungus powder is as follows: Weigh each edible fungus according to the ratio, pulverize the compound edible fungus to a particle size of less than 0.5 mm to obtain pulverized material, and treat it with microwave irradiation at a power of 290W for 80s during the pulverization process. Add the pulverized material and 17.5 times the weight of water to a container, and then add 1.5 times the weight of carbon nanotubes to the pulverized material. Install positive and negative electrodes on both sides of the container, and stir while applying electricity for 7.5 minutes to obtain a mixture. The voltage of the electricity is 30V and the current is 3A. Heat the mixture to 55℃, and then add 0.2% of the mass of cellulase of the pulverized material for enzymatic hydrolysis for 5 minutes. Adjust the temperature to 85℃ and maintain it for 3.5 minutes. Then adjust the temperature to 50℃, and then add 0.2% of the mass of flavor protease of the pulverized material for enzymatic hydrolysis for 15 minutes. Centrifuge to obtain the supernatant, and spray dry the supernatant to obtain the compound edible fungus powder.

[0035] In this embodiment, the preparation method of the Eucommia ulmoides extract is as follows: Eucommia ulmoides is taken, 15 times the weight of deionized water is added, and it is extracted 3 times at 95°C, with each extraction time being 2.5 hours. The extract is filtered, combined, and concentrated to a solid content of 7.5% to obtain Eucommia ulmoides extract concentrate. The Eucommia ulmoides extract concentrate is dried to obtain Eucommia ulmoides extract.

[0036] Preferably, the kelp extract is prepared as follows: kelp is added to 12.5 times its weight of deionized water, and high-pressure pulse extraction is performed for 40 min under the conditions of field strength of 15 kV / cm, pulse time of 600 μs, and pulse frequency of 300 Hz. After filtration, the extract is spray-dried to obtain the kelp extract.

[0037] In the specific implementation, the extract of Eupatorium fortunei was obtained by reflux extraction with 70% ethanol by mass percentage. The material-to-liquid ratio during the extraction process was 1:13, the extraction temperature was 80℃, the extraction was performed twice, and the extraction time was 85 min. After extraction, the extract was concentrated to a thick paste with a relative density of 1.15, and finally spray-dried to obtain the extract of Eupatorium fortunei.

[0038] In this embodiment, the preparation method of the Astragalus extract is as follows: Astragalus is taken, 10 times its weight of deionized water is added, and the mixture is extracted 3 times at a temperature of 45°C and a power of 200W, with each extraction time being 40 minutes. The extracted extract is concentrated to a thick paste with a relative density of 1.15, and finally spray-dried to obtain the Astragalus extract.

[0039] In this embodiment, the enzyme compound microbial agent is a mixture of yeast, acetic acid bacteria, and lactic acid bacteria; the weight ratio of yeast, lactic acid bacteria, and acetic acid bacteria is 1:1:1.5, and the colony count of each of the yeast, lactic acid bacteria, and acetic acid bacteria is 10. 9 cfu / g.

[0040] Example 4 This invention provides an edible fungus polysaccharide health food for combating fatigue and enhancing immunity, comprising the following raw materials in parts by weight: 95 parts of compound edible fungus powder, 18 parts of Eucommia ulmoides extract, 22 parts of kelp extract, 25 parts of Lycopus lucidus extract, 30 parts of Astragalus membranaceus extract, and 0.4 parts of enzyme compound microbial agent. The compound edible fungus powder includes Armillaria mellea powder, Hydrangea macrophylla powder, Pleurotus ostreatus powder, and Dictyophora indicum powder, in a mass ratio of 4.5:2.5:1.8:1. The preparation method of the edible fungus polysaccharide health food for combating fatigue and enhancing immunity is as follows: Figure 1 As shown, it includes the following steps: Prepare all raw materials according to the specified proportions; After mixing all the ingredients evenly, sterilize them using microwave. The mixture after microwave sterilization is packaged to obtain health food.

[0041] The preparation method of the compound edible fungus powder is as follows: Weigh each edible fungus according to the ratio, crush the compound edible fungus to a particle size of less than 0.5 mm to obtain pulverized material, and treat it with microwave irradiation at a power of 300W for 100s during the crushing process. Add the pulverized material and 20 times the weight of water to a container, and then add carbon nanotubes with a weight of 2 times the weight of the pulverized material. Install positive and negative electrodes on both sides of the container, and stir while applying electricity for 10 minutes to obtain a mixture. The voltage of the electricity is 40V and the current is 5A. Heat the mixture to 60℃, and then add 0.3% of the mass of cellulase of the pulverized material for enzymatic hydrolysis for 30 minutes. Adjust the temperature to 90℃ and hold for 5 minutes. Then adjust the temperature to 55℃, and then add 0.3% of the mass of flavor protease of the pulverized material for enzymatic hydrolysis for 20 minutes. Centrifuge to obtain the supernatant, and spray dry the supernatant to obtain the compound edible fungus powder.

[0042] In this embodiment, the preparation method of the Eucommia ulmoides extract is as follows: take Eucommia ulmoides, add 20 times the weight of deionized water, extract 3 times at 100℃, each extraction time is 3h, filter the extract, combine, concentrate to the solid content of 10%, and obtain Eucommia ulmoides extract concentrate. The Eucommia ulmoides extract concentrate is dried to obtain Eucommia ulmoides extract.

[0043] Preferably, the kelp extract is prepared as follows: kelp is added to 15 times its weight of deionized water, and high-pressure pulse extraction is performed for 50 min under the conditions of field strength of 20 kV / cm, pulse time of 700 μs, and pulse frequency of 400 Hz. After filtration, the extract is spray-dried to obtain the kelp extract.

[0044] In the specific implementation, the extract of Eupatorium fortunei was obtained by reflux extraction with 80% ethanol by mass percentage. The material-to-liquid ratio during the extraction process was 1:15, the extraction temperature was 85℃, the extraction was performed twice, and the extraction time was 100 min. After extraction, the extract was concentrated to a thick paste with a relative density of 1.2, and finally spray-dried to obtain the extract of Eupatorium fortunei.

[0045] In this embodiment, the preparation method of the Astragalus extract is as follows: Astragalus is taken, 12 times its weight of deionized water is added, and the mixture is extracted 3 times at a temperature of 50°C and a power of 250W, with each extraction time being 50 minutes. The extracted extract is concentrated to a thick paste with a relative density of 1.2, and finally spray-dried to obtain the Astragalus extract.

[0046] In this embodiment, the enzyme compound microbial agent is a mixture of yeast, acetic acid bacteria, and lactic acid bacteria; the weight ratio of yeast, lactic acid bacteria, and acetic acid bacteria is 1:1.5:2, and the colony count of each of the yeast, lactic acid bacteria, and acetic acid bacteria is 10. 10 cfu / g.

[0047] Example 5 This invention provides an edible fungus polysaccharide health food for combating fatigue and enhancing immunity, comprising the following raw materials in parts by weight: 100 parts of compound edible fungus powder, 20 parts of Eucommia ulmoides extract, 25 parts of kelp extract, 30 parts of Lycopus lucidus extract, 40 parts of Astragalus membranaceus extract, and 0.5 parts of enzyme compound microbial agent. The compound edible fungus powder includes Armillaria mellea powder, Hydrangea macrophylla powder, Pteris vittata powder, and Dictyophora indicum powder, in a mass ratio of 5:3:2:1. The preparation method of the edible fungus polysaccharide health food for combating fatigue and enhancing immunity is as follows: Figure 1 As shown, it includes the following steps: Prepare all raw materials according to the specified proportions; After mixing all the ingredients evenly, sterilize them using microwave. The mixture after microwave sterilization is packaged to obtain health food.

[0048] The preparation method of the compound edible fungus powder is as follows: Weigh each edible fungus according to the ratio, crush the compound edible fungus to a particle size of less than 0.5 mm to obtain pulverized material, and treat it with microwave irradiation at a power of 300W for 100s during the crushing process. Add the pulverized material and 20 times the weight of water to a container, and then add carbon nanotubes with a weight of 2 times the weight of the pulverized material. Install positive and negative electrodes on both sides of the container, and stir while applying electricity for 10 minutes to obtain a mixture. The voltage of the electricity is 40V and the current is 5A. Heat the mixture to 60℃, and then add 0.3% of the mass of cellulase of the pulverized material for enzymatic hydrolysis for 30 minutes. Adjust the temperature to 90℃ and hold for 5 minutes. Then adjust the temperature to 55℃, and then add 0.3% of the mass of flavor protease of the pulverized material for enzymatic hydrolysis for 20 minutes. Centrifuge to obtain the supernatant, and spray dry the supernatant to obtain the compound edible fungus powder.

[0049] In this embodiment, the preparation method of the Eucommia ulmoides extract is as follows: take Eucommia ulmoides, add 20 times the weight of deionized water, extract 3 times at 100℃, each extraction time is 3h, filter the extract, combine, concentrate to the solid content of 10%, and obtain Eucommia ulmoides extract concentrate. The Eucommia ulmoides extract concentrate is dried to obtain Eucommia ulmoides extract.

[0050] Preferably, the kelp extract is prepared as follows: kelp is added to 15 times its weight of deionized water, and high-pressure pulse extraction is performed for 50 min under the conditions of field strength of 20 kV / cm, pulse time of 700 μs, and pulse frequency of 400 Hz. After filtration, the extract is spray-dried to obtain the kelp extract.

[0051] In the specific implementation, the extract of Eupatorium fortunei was obtained by reflux extraction with 80% ethanol by mass percentage. The material-to-liquid ratio during the extraction process was 1:15, the extraction temperature was 85℃, the extraction was performed twice, and the extraction time was 100 min. After extraction, the extract was concentrated to a thick paste with a relative density of 1.2, and finally spray-dried to obtain the extract of Eupatorium fortunei.

[0052] In this embodiment, the preparation method of the Astragalus extract is as follows: Astragalus is taken, 12 times its weight of deionized water is added, and the mixture is extracted 3 times at a temperature of 50°C and a power of 250W, with each extraction time being 50 minutes. The extracted extract is concentrated to a thick paste with a relative density of 1.2, and finally spray-dried to obtain the Astragalus extract.

[0053] In this embodiment, the enzyme compound microbial agent is a mixture of yeast, acetic acid bacteria, and lactic acid bacteria; the weight ratio of yeast, lactic acid bacteria, and acetic acid bacteria is 1:1.5:2, and the colony count of each of the yeast, lactic acid bacteria, and acetic acid bacteria is 10. 10 cfu / g.

[0054] Comparative Example 1 Compared to Example 3, the Armillaria mellea powder was replaced with other types of fungal powder without changing the proportions of the other types of fungal powder.

[0055] Comparative Example 2 Compared to Example 3, the hydrangea powder was replaced with other types of powder without changing the proportions of the other types of powder.

[0056] Comparative Example 3 Compared to Example 3, the tiger paw fungus powder was replaced with other types of fungal powder without changing the proportions of the other types of fungal powder.

[0057] Comparative Example 4 Compared to Example 3, bamboo fungus powder was replaced with other types of fungal powder without changing the proportions of the other types of fungal powder.

[0058] Comparative Example 5 Compared to Example 3, the edible mushroom powder was replaced with an equal amount of deionized water.

[0059] Experiment 1: Fatigue Resistance Experiment Experimental methods: Seven-week-old male ICR mice (18-22g) were used as experimental subjects. The mice were randomly assigned to a blank group (the blank group was given physiological saline) and 10 experimental groups (corresponding to the products of Examples 1-5 and Comparative Examples 1-5, respectively), with 10 mice in each group. The mice were given the test drug by gavage once a day at a dose of 0.15g / Kg for 15 days. Then the mice were subjected to a weighted swimming experiment.

[0060] Test method: 30 minutes after the last administration, lead sheets (5% of the mouse's body weight) were placed on the tails of the mice. One mouse was randomly selected from each group and placed in water. Timing began and ended when the mouse reached exhaustion. The time of the mouse's first sinking and the time of exhaustion were recorded. The difference between these two times represents the mouse's endurance. Testing container: Use a water bucket with a diameter of 50cm and a height of 45cm, with a water level of 30cm and a water temperature of 24-26℃, 4 buckets per bucket.

[0061] Testing standards: The first sinking standard is when the tip of the mouse's nose first goes below the water surface, and the exhaustion standard is when the mouse sinks underwater for 10 seconds without surfacing.

[0062] The experimental results are shown in Table 1 below: Table 1. Results of fatigue resistance test The results above show that the edible fungi health food prepared by this invention has a good anti-fatigue effect. The *Armillaria mellea* powder, *Hydrangea macrophylla* powder, *Tiger Paw* powder, and *Dictyophora indica* powder in the edible fungi powder have a synergistic effect, which can further enhance the anti-fatigue effect.

[0063] Experiment 2: Immunity Experiment Experimental Methods: Seven-week-old male ICR mice (18-22g) were used as experimental subjects and randomly divided into groups of 10 mice each. The experimental groups were given the products prepared in Examples 1-5 and Comparative Examples 1-5, while the control group was given physiological saline. Based on body weight, the experimental groups were administered the product by gavage at a dosage of 0.04g / kg; the control group was given the same volume of physiological saline. This was repeated for 10 consecutive days by gavage.

[0064] The effects of the beverage on the immunity of mice were evaluated using the following indicators: (1) Immune organ index: Mice were euthanized by cervical dislocation, and the thymus and spleen were removed, weighed, and the thymus index and spleen index were calculated. Thymus index = thymus weight / body weight; Spleen index = spleen weight / body weight. The experimental results are shown in Table 2.

[0065] Table 2 Results of the Immune Organ Index Experiment The results above show that the thymus and spleen organ indices of Examples 1-5 and Comparative Examples 1-5 were significantly increased. The effect of Examples 1-5 was better than that of Comparative Examples 1-5. The product of the present invention has the effect of improving immunity. Furthermore, the *Armillaria mellea* powder, *Hydrangea macrophylla* powder, *Tiger Paw* powder, and *Dictyophora indica* powder in the edible fungus powder have a synergistic effect, which can further improve immunity.

[0066] In summary, this invention provides an edible fungus polysaccharide health food that combats fatigue and enhances immunity, comprising: It should be noted that, for the sake of simplicity, the foregoing embodiments are all described as a series of actions. However, those skilled in the art should understand that the present invention is not limited to the described order of actions, as some steps may be performed in other orders or simultaneously according to the present invention. Furthermore, those skilled in the art should also understand that the embodiments described in the specification are preferred embodiments, and the actions and modules involved are not necessarily essential to the present invention.

[0067] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit the scope of protection of the invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on these embodiments, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art can still combine, add, delete, or otherwise adjust the features of the various embodiments of the present invention according to the circumstances without conflict or creative effort, thereby obtaining different technical solutions that do not fundamentally depart from the concept of the present invention. These technical solutions also fall within the scope of protection of the present invention.

Claims

1. A health food product made from edible fungi polysaccharides that combats fatigue and enhances immunity, characterized in that, The ingredients include the following raw materials in parts by weight: 80-100 parts of compound edible fungus powder, 10-20 parts of Eucommia ulmoides extract, 15-25 parts of kelp extract, 10-30 parts of Eupatorium fortunei extract, 5-40 parts of Astragalus membranaceus extract, and 0.1-0.5 parts of enzyme compound microbial agent. The compound edible fungus powder includes Armillaria mellea powder, Hydrangea macrophylla powder, Pteris vittata powder, and Dictyophora indica powder, with a mass ratio of 2-5:1-3:1-2:

1.

2. The edible fungi polysaccharide health food for anti-fatigue and immunity enhancement as described in claim 1, characterized in that, The product comprises the following raw materials in parts by weight: 85-95 parts of compound edible fungus powder, 12-18 parts of Eucommia ulmoides extract, 18-22 parts of kelp extract, 15-25 parts of Eupatorium fortunei extract, 15-30 parts of Astragalus membranaceus extract, and 0.2-0.4 parts of enzyme compound microbial agent. The compound edible fungus powder includes Armillaria mellea powder, Hydrangea macrophylla powder, Pteris vittata powder, and Dictyophora indica powder, with a mass ratio of 2.5-4.5:1.5-2.5:1.2-1.8:

1.

3. The edible fungi polysaccharide health food for anti-fatigue and immunity enhancement as described in claim 2, characterized in that, The ingredients include the following raw materials in parts by weight: 90 parts of compound edible fungus powder, 15 parts of Eucommia ulmoides extract, 20 parts of kelp extract, 20 parts of Lycopus lucidus extract, 22.5 parts of Astragalus membranaceus extract, and 0.3 parts of enzyme compound microbial agent. The compound edible fungus powder includes Armillaria mellea powder, Hydrangea macrophylla powder, Pteris vittata powder, and Dictyophora indica powder, with a mass ratio of 3.5:2:1.5:

1.

4. The edible fungi polysaccharide health food for anti-fatigue and immunity enhancement as described in claim 1, characterized in that, The preparation method of the compound edible fungus powder is as follows: Weigh each edible fungus according to the ratio, pulverize the compound edible fungus to a particle size of less than 0.5 mm to obtain pulverized material. During the pulverization process, microwave irradiation with a power of 280-300W is used for 60-100s. Add the pulverized material and 15-20 times the weight of water to a container, and then add 1-2 times the weight of carbon nanotubes to the pulverized material. Install positive and negative electrodes on both sides of the container, and stir while applying electricity for 5-10 minutes to obtain a mixture. The voltage of the electricity is 20-40V and the current is 1-5A. Heat the mixture to 50-60℃, and then add 0.1-0.3% of cellulase by weight of the pulverized material for enzymatic hydrolysis for 20-30 minutes. Adjust the temperature to 80-90℃ and maintain it for 2-5 minutes. Then adjust the temperature to 45-55℃, and then add 0.1-0.3% of flavor protease by weight of the pulverized material for enzymatic hydrolysis for 10-20 minutes. Centrifuge to obtain the supernatant, filter the supernatant and spray dry it to obtain the compound edible fungus powder.

5. The edible fungi polysaccharide health food for anti-fatigue and immune enhancement as described in claim 4, characterized in that, The preparation method of the Eucommia ulmoides extract is as follows: Take Eucommia ulmoides, add 10-20 times the weight of deionized water, extract 3 times at 90-100℃, each extraction time is 2-3 hours, filter the extract, combine, concentrate to the solid content of 5-10%, and obtain Eucommia ulmoides extract concentrate. Dry the Eucommia ulmoides extract concentrate to obtain Eucommia ulmoides extract.

6. The edible fungi polysaccharide health food for anti-fatigue and immune enhancement as described in claim 1, characterized in that, The preparation method of the kelp extract is as follows: Take kelp, add 10-15 times its weight of deionized water, and perform high-pressure pulse extraction for 30-50 min under the conditions of field strength of 10-20 kV / cm, pulse time of 500-700 μs, and pulse frequency of 200-400 Hz. After filtration, spray dry to obtain kelp extract.

7. The edible fungi polysaccharide health food for anti-fatigue and immunity enhancement as described in claim 6, characterized in that, The extract of *Eupatorium fortunei* was obtained by reflux extraction with 60-80% ethanol (by mass). The material-to-liquid ratio during extraction was 1:10-15, the extraction temperature was 75-85℃, the extraction was performed twice, and the extraction time was 70-100 min. After extraction, the extract was concentrated to a thick paste with a relative density of 1.1-1.2, and finally spray-dried to obtain the extract of *Eupatorium fortunei*.

8. The edible fungi polysaccharide health food for anti-fatigue and immunity enhancement as described in claim 1, characterized in that, The preparation method of the Astragalus extract is as follows: Take Astragalus, add 8-12 times the weight of deionized water, extract 2-3 times at a temperature of 40-50℃ and a power of 150-250W, each extraction time is 30-50min, concentrate the extract obtained after extraction to a thick paste with a relative density of 1.1-1.2, and finally spray dry to obtain Astragalus extract.

9. The edible fungi polysaccharide health food for anti-fatigue and immunity enhancement as described in claim 1, characterized in that, The enzyme compound microbial agent is a mixture of yeast, acetic acid bacteria, and lactic acid bacteria; the weight ratio of yeast, lactic acid bacteria, and acetic acid bacteria is 1:0.5-1.5:1-2, and the colony count of each of the yeast, lactic acid bacteria, and acetic acid bacteria is 10. 8 -10 10 cfu / g.

10. The preparation method of the edible fungi polysaccharide health food for anti-fatigue and immune enhancement as described in any one of claims 1-9, characterized in that, Includes the following steps: Prepare all raw materials according to the specified proportions; After mixing all the ingredients evenly, sterilize them using microwave. The mixture after microwave sterilization is packaged to obtain health food.

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