Liquid phase sample tomography fluorescence continuous scanning device based on piezoelectric zooming and application

Abstract

The invention relates to a liquid phase sample tomography fluorescence continuous scanning device based on piezoelectric zooming and application, and the device comprises a light splitting dichroscope, an incident plane matched with the light splitting dichroscope is provided with a light inlet channel and a reflection channel, and an emergent plane matched with the light splitting dichroscope is provided with a detection channel. A light emitting unit, a sample position and a detection unit are respectively matched with the light inlet channel, the reflection channel and the detection channel; a piezoelectric zooming assembly is also arranged in cooperation with the reflection channel and is used for exciting concentration marks of to-be-detected objects of different faults of the liquid phase sample; the method is applied to magnetic particle liquid-phase fluorescence tomography continuous scanning detection. According to the invention, the piezoelectric component material is adopted, the contraction and extension of the component in the length direction are controlled through the change of voltage, the focal length change in a larger range can be completed by only needing smaller voltage, the up-and-down sampling requirement of the vertical section of sampling liquid in the reaction cup can be completely met, and a more accurate fluorescence signal measurement result can be obtained; the volume of the piezoelectric component material is small, and the piezoelectric component material is very easy to realize in a small space for precise measurement.

Description

Technical Field

[0001] This invention relates to the field of measurement and testing, and in particular to a piezoelectric zoom-based liquid phase sample tomographic fluorescence continuous scanning device and its application. Background Technology

[0002] In a liquid-phase immunoassay system with a single reaction cup, magnetic microspheres made of paramagnetic material are typically used as a solid-phase carrier to adsorb the target antigen or antibody. Then, a secondary antibody with a fluorescent label is attached. The magnetic beads are adsorbed onto the side wall of the reaction cup by a permanent magnet or electromagnet. The liquid inside the reaction cup is then evacuated by inserting a syringe to remove the unbound fluorescent label from the liquid. A diluent is then injected to re-mix the magnetic beads on the tube wall into the solution. The concentration of the target analyte is calculated by detecting the remaining fluorescent label of the secondary antibody adsorbed on the magnetic beads.

[0003] Traditional liquid chromatography fluorescence detection methods typically involve reflecting the excitation source through a spectrophotometer, focusing it onto a fixed point below the surface of the sample solution using a convex lens, and then focusing the fluorescence signal intensity onto the sensor after passing through a filter and the spectrophotometer. Magnetic beads, made primarily of iron oxide (Fe3O4), are microspheres with a diameter generally greater than 1 μm. Since the specific gravity of the beads is greater than that of the diluent, they gradually sink, causing the fluorescent markers bound to the magnetic particles to also settle, resulting in a concentration gradient at the solution surface. However, in traditional methods, the focal point of the single lens on the sample solution is fixed. Since the distribution of magnetic beads in the sample solution cannot be uniformly distributed throughout, the detection results are not representative—variable focal points introduce measurement errors.

[0004] Therefore, a more accurate method for measuring fluorescence signals should sample the entire vertical profile of the solution sample. To achieve vertical profile sampling of liquid samples, the detection optical structure needs to have zoom capabilities. Existing optical solutions often use mechanical structures to move the lens, thereby achieving vertical movement of the focal point. This requires the cooperation of motors and lead screw structures, which often results in high structural complexity, large size, and impracticality in the small spaces required for precision measurements. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a piezoelectric zoom-based liquid phase sample tomographic fluorescence continuous scanning device and its application.

[0006] The technical solution adopted in this invention is a piezoelectric zoom-based liquid phase sample tomographic fluorescence continuous scanning device. The device includes a beam splitter mirror, with an incident light channel and a reflection channel on the incident surface of the beam splitter mirror, and a detection channel on the exit surface of the beam splitter mirror. A light-emitting unit, a sample position, and a detection unit are respectively provided in conjunction with the incident light channel, the reflection channel, and the detection channel.

[0007] The reflection channel is also equipped with a piezoelectric zoom component, which is used to excite the concentration marking of the analyte in different sections of the liquid phase sample.

[0008] Preferably, the piezoelectric zoom assembly includes a housing, with a plano lens at the upper and lower ends of the housing, a first convex lens and a second convex lens inside the housing, and a piezoelectric unit in conjunction with the first and second convex lenses.

[0009] Preferably, the piezoelectric unit includes a bracket that is respectively arranged to cooperate with the opposing surfaces of the first convex lens and the second convex lens. The bracket has a light-transmitting hole at its center. A piezoelectric material is arranged between the two brackets, and a voltage generator is arranged in cooperation with the piezoelectric material.

[0010] Preferably, the piezoelectric material is evenly distributed on the outer edge of the support.

[0011] Preferably, the light-emitting unit includes an excitation light source, and a collimating lens is provided between the excitation light source and the beam splitter.

[0012] Preferably, the beam splitter has a blind hole on the other side of the light-incident channel.

[0013] Preferably, the detection unit includes a photosensitive sensor located at the end of the detection channel.

[0014] Preferably, a filter and a lens are sequentially arranged between the beam splitter and the photosensitive sensor.

[0015] An application of a piezoelectric zoom-based liquid phase sample tomographic fluorescence continuous scanning device, applied to the detection of magnetic microparticle liquid phase fluorescence tomographic continuous scanning.

[0016] Preferably, the actual detection focal length of the continuous tomographic scan meets the following requirements:

[0017]

[0018] in, and These are the focal lengths of the two convex lenses in the piezoelectric zoom assembly. The real-time distance between the two convex lenses is given.

[0019] This invention relates to a piezoelectric zoom-based continuous scanning device for liquid phase sample tomography fluorescence and its application. It includes a beam splitter, with an incident light channel and a reflection channel on the incident surface of the beam splitter, and a detection channel on the exit surface of the beam splitter. A light-emitting unit, a sample position, and a detection unit are respectively provided in the incident light channel, the reflection channel, and the detection channel. A piezoelectric zoom component is also provided in conjunction with the reflection channel to excite analyte concentration markers in different sections of the liquid phase sample. It is applied to continuous scanning detection of magnetic particle liquid phase fluorescence tomography.

[0020] The beneficial effects of this invention are that by using piezoelectric component materials, the contraction and elongation of the component in the length direction can be controlled by the change of voltage, thereby achieving the change of the focal length of the lens group. Only a small voltage is needed to complete the focal length change over a large range, which can fully meet the vertical sampling requirements of the sampled liquid in the reaction cup, thereby obtaining more accurate fluorescence signal measurement results. The piezoelectric component material is also small in size, making it very easy to implement in a small space for precision measurement. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of the present invention, wherein the arrows indicate the direction of light rays;

[0022] Figure 2 This is a schematic diagram of the structure of the piezoelectric zoom assembly of the present invention;

[0023] Figure 3 This is a schematic diagram of the structure of the piezoelectric unit of the present invention in conjunction with the first convex lens and the second convex lens;

[0024] Figure 4 This is a schematic diagram illustrating the working principle of the first and second convex lenses of the present invention. Detailed Implementation

[0025] The present invention will be further described in detail below with reference to embodiments, but the scope of protection of the present invention is not limited thereto.

[0026] This invention relates to a piezoelectric zoom-based liquid phase sample tomographic fluorescence continuous scanning device. The device includes a beam splitter 1, an incident light channel 2 and a reflection channel 3 on the incident surface of the beam splitter 1, a detection channel 5 on the exit surface of the beam splitter 1, and a light emission unit 6, a sample position 7 and a detection unit 8 respectively on the incident light channel 2, the reflection channel 3 and the detection channel 5.

[0027] The reflection channel 3 is also equipped with a piezoelectric zoom component 9, which is used to excite the concentration marking of the analyte in different sections of the liquid phase sample.

[0028] In this invention, by setting up a piezoelectric component 9, the piezoelectric component material in the piezoelectric zoom component 9 is controlled to contract and elongate in the length direction based on the change of voltage, thereby achieving the goal of changing the focal length of the lens group, and then collecting the concentration labeling information of the analyte in different layers of the liquid sample. Here, the concentration labeling refers to fluorescence.

[0029] In this invention, it is obvious that the reflection channel 3 and the detection channel 5 are located on the same axis.

[0030] The piezoelectric zoom assembly 9 includes a housing 91, with a flat lens 92 provided at the upper and lower ends of the housing 91, a first convex lens 93 and a second convex lens 94 provided inside the housing 91, and a piezoelectric unit provided in conjunction with the first convex lens 93 and the second convex lens 94.

[0031] The piezoelectric unit includes a bracket 95 that is respectively arranged on the opposite surfaces of the first convex lens 93 and the second convex lens 94. The bracket 95 has a light-transmitting hole 96 at its center. A piezoelectric material 97 is arranged between the two brackets 95. A voltage generator (not shown in the figure) is arranged in conjunction with the piezoelectric material 97.

[0032] The piezoelectric material 97 is evenly distributed on the outer edge of the support 95.

[0033] Specifically, in this invention, the encapsulation shell 91 ensures the overall waterproofing of the component, and flat mirrors 92 are provided at the top and bottom ends to ensure normal light transmission; two convex lenses 93 and 94 connected by piezoelectric units are provided inside the encapsulation shell 91. Different voltages applied by a voltage generator act on the piezoelectric material 97, thereby changing the distance between the two supports 95, which in turn changes the distance between the first convex lens 93 and the second convex lens 94, thus changing the overall focal length.

[0034] To ensure that the piezoelectric component material does not affect the actual light transmission effect, in addition to the flat mirrors 92 set at the top and bottom of the encapsulation shell 91, the piezoelectric material 97 is also uniformly set on the outer edge of the bracket 95, so that the light-transmitting hole 96 at the center of the bracket 95 can completely pass through the reflected fluorescence.

[0035] The light-emitting unit 6 includes an excitation light source, and a collimating lens 4 is provided between the excitation light source and the beam splitter.

[0036] The beam splitter 1 has a blind hole (not shown in the figure) on the other side of the light-incident channel 2.

[0037] The detection unit 8 includes a photosensitive sensor located at the end of the detection channel 5.

[0038] A filter 10 and a lens 11 are sequentially arranged between the beam splitter 1 and the photosensitive sensor.

[0039] In this invention, the excitation light emitted from the excitation light source is calibrated by the collimating lens 4 and then directed to the beam splitter 1. The beam splitter 1 reflects the excitation light to the sample position 7. After the sample in the sample position 7 is excited to fluoresce, it is zoomed by the piezoelectric zoom component 9. The fluorescence information of different sections is sampled and reflected back through the filter 10 for filtering and the lens 11 for focusing before being detected by the photosensitive sensor.

[0040] In this invention, the channel for exciting the sample coincides with the detection channel 5 and is perpendicular to the incident light channel 2. The incident surface of the beam splitter 1 faces the incident light channel 2, and the transmission surface (exit surface) faces the detection channel 5.

[0041] This invention also relates to the application of a piezoelectric zoom-based liquid phase sample tomographic fluorescence continuous scanning device, which is used for magnetic particle liquid phase fluorescence tomographic continuous scanning detection.

[0042] The actual detection focal length of the continuous tomographic scan meets the requirements.

[0043]

[0044] in, and These are the focal lengths of the two convex lenses 93 and 94 of the piezoelectric zoom assembly 9, respectively. The real-time distance between the two convex lenses 93 and 94.

[0045] In this invention, the focal length of the lens group and the focal length of the composed lens are... and The focal length of a piezoelectric component is related to its distance d. When the material is driven by voltage, the magnitude of d changes, which in turn changes the overall focal length. Unlike existing technologies that change the focal length of one lens by using a piezoelectric component, the piezoelectric unit of this invention is installed between the first convex lens 93 and the second convex lens 94. By changing the distance of the combined focal point, the entire lens group can achieve long-distance zoom and magnify the piezoelectric deformation distance.

[0046] The following is an example:

[0047] The lens group uses convex lenses of 93 and 94, with a focal length of... and Both are 8mm long. Under normal conditions without voltage, the length of the piezoelectric material 97 is 12mm. What is the focal length of the combined material? It is 16mm;

[0048] When a positive voltage is applied, the piezoelectric material 97 can elongate to a maximum height of 14 mm, thereby changing the focal length of the combined lens to 32 mm.

[0049] When a reverse voltage is applied, its minimum height is 10mm, thus making the focal length of the combined lens 10.6mm;

[0050] As can be seen from the above embodiments, by driving the piezoelectric component material to expand and contract with voltage, the focal length of the lens group can be varied from 10.6mm to 32mm, with a variation range of 21.4mm, which can fully meet the vertical sampling requirements of the sampled liquid in the reaction cup.

[0051] Although preferred embodiments of the invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the invention.

[0052] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. A continuous scanning device for liquid phase sample tomography fluorescence based on piezoelectric zoom, characterized in that: The device includes a beam splitter, with an incident light channel and a reflection channel on the incident surface of the beam splitter, and a detection channel on the exit surface of the beam splitter. A light-emitting unit, a sample position, and a detection unit are respectively provided in conjunction with the incident light channel, the reflection channel, and the detection channel. The reflection channel is also equipped with a piezoelectric zoom component, which is used to excite the concentration marking of the analyte in different sections of the liquid phase sample.

2. The continuous scanning device for liquid phase sample tomography fluorescence based on piezoelectric zoom according to claim 1, characterized in that: The piezoelectric zoom assembly includes a housing, with a flat lens at the upper and lower ends of the housing, a first convex lens and a second convex lens inside the housing, and a piezoelectric unit in conjunction with the first and second convex lenses.

3. The continuous scanning device for liquid phase sample tomography fluorescence based on piezoelectric zoom according to claim 2, characterized in that: The piezoelectric unit includes a bracket arranged on the opposite surfaces of the first convex lens and the second convex lens respectively. The bracket has a light-transmitting hole at its center. A piezoelectric material is arranged between the two brackets. A voltage generator is arranged in conjunction with the piezoelectric material.

4. The continuous scanning device for liquid phase sample tomography fluorescence based on piezoelectric zoom according to claim 3, characterized in that: The piezoelectric material is evenly distributed on the outer edge of the support.

5. The continuous scanning device for liquid phase sample tomography fluorescence based on piezoelectric zoom according to claim 1, characterized in that: The light-emitting unit includes an excitation light source, and a collimating lens is provided between the excitation light source and the beam splitter.

6. The continuous scanning device for liquid phase sample tomography fluorescence based on piezoelectric zoom according to claim 1, characterized in that: The beam splitter has a blind hole on the other side of the light inlet channel.

7. The continuous scanning device for liquid phase sample tomography fluorescence based on piezoelectric zoom according to claim 1, characterized in that: The detection unit includes a photosensitive sensor located at the end of the detection channel.

8. The continuous scanning device for liquid phase sample tomography fluorescence based on piezoelectric zoom according to claim 7, characterized in that: A filter and a lens are sequentially arranged between the beam splitter and the photosensitive sensor.

9. An application of a piezoelectric zoom-based liquid phase sample tomographic fluorescence continuous scanning device, characterized in that: It is applied to the continuous scanning detection of magnetic microparticle liquid phase fluorescence tomography.

10. The application according to claim 9, characterized in that: The actual detection focal length of the continuous tomographic scan meets the requirements. ， in, and These are the focal lengths of the two convex lenses in the piezoelectric zoom assembly. The real-time distance between the two convex lenses is given.

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