Nano composition of pterostilbene, preparation method of nano composition and application of pterostilbene in preparation of anti-alopecia products

Abstract

The invention discloses a nano composition of pterostilbene, a preparation method of the nano composition, and application of the pterostilbene in preparation of an anti-hair loss product. Comprising the following components in percentage by mass: 0.1-25% of pterostilbene, 0.1-30% of polyol ether, 0.1-8% of terpene essential oil, 0.5-30% of a flexible agent, 0.1-20% of phospholipid and / or phospholipid derivative, 0-2% of honokiol, 0-3% of cinnamic acid, 0-35% of an additive and the balance of water. The invention proves that the pterostilbene has an excellent anti-hair loss effect and has relatively strong 5 alpha-reductase inhibitory activity, and the nano composition containing the pterostilbene, honokiol and cinnamic acid can synergistically enhance the 5 alpha-reductase inhibitory and anti-hair loss effects of the pterostilbene, and meanwhile, the nano composition enhances the solubility and stability of the pterostilbene, so that the anti-hair loss effect of the pterostilbene is improved, and the anti-hair loss effect of the pterostilbene is improved. The application of the pterostilbene is expanded.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics and dermatology, and particularly relates to a nano composition of pterostilbene, a preparation method thereof and application of pterostilbene in preparation of anti-hair loss products. BACKGROUND

[0002] With the increase of work pressure, the acceleration of life rhythm, environmental influence and the use of hair styling products such as hair dyeing and perming, hair loss shows signs of youth, and young people have become the main force of hair loss, which has a significant impact on personal mental health and life quality, and safe and effective anti-hair loss products are urgently needed.

[0003] At present, although there are many anti-hair loss products on the market, due to the unclear research on the causes of hair loss, the uncertain content of plant extract ingredients, the unknown mechanism of action, the disordered compatibility of the components in the existing anti-hair loss essence, the poor rationality, the slow effect and the short effective period, it is urgent to develop a safe and effective anti-hair loss product.

[0004] Androgenic alopecia, also known as seborrheic alopecia, is related to at least 95% of male hair loss. The mechanism is that 5α-reductase in hair follicle converts testosterone into more active dihydrotestosterone (DHT). DHT binds to androgen receptors in hair follicles, leading to miniaturization of hair follicles and causing hair loss.

[0005] In addition, studies have found that the activity of 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-hydroxysteroid dehydrogenase (17β-HSD) type 5 in hair loss follicles significantly increases, which can convert androgen precursors into testosterone, and then convert them into DHT by 5α-reductase, thereby affecting hair loss.

[0006] Pterostilbene, also known as 3', 5'-dimethoxy-4-hydroxystilbene, is a natural polyphenolic compound with excellent antioxidant, anti-inflammatory, melanin production inhibition and whitening effects. As a safe and effective new ingredient, pterostilbene completed the new cosmetic raw material filing in CFDA in 2024. The inventors of the present application accidentally found that pterostilbene has excellent 5α-reductase inhibitory activity, but its phenolic hydroxyl group is unstable and cannot be maintained for a long time in light and air. After oxidation, the color becomes darker, and the water solubility is poor. Therefore, it is urgent to develop a nano composition of pterostilbene to solve these problems. SUMMARY

[0007] In order to solve the problems raised in the background art, the purpose of the present application is to provide a nano composition of pterostilbene, a preparation method thereof and application of pterostilbene in preparation of anti-hair loss products.

[0008] In order to achieve the above object, the technical scheme adopted by the present application is: on the one hand, the present application provides a nano composition of pterostilbene, which comprises the following components in mass percentage: pterostilbene 0.1-25%, polyol ether 0.1-30%, terpene essential oil 0.1-8%, flexible agent 0.5-30%, phospholipid and / or phospholipid derivative 0.1-20%, and honokiol 0-2%, cinnamic acid 0-3%, additive 0-35%, and the balance is water.

[0009] Further, it comprises the following components in mass percentage: pterostilbene 0.1-25%, polyol ether 0.1-30%, terpene essential oil 0.1-8%, flexible agent 0.5-30%, phospholipid and / or phospholipid derivative 0.1-20%, and honokiol 0.1-2%, cinnamic acid 0.1-3%, additive 0-35%, and the balance is water.

[0010] Further, it comprises the following components in mass percentage: pterostilbene 0.1-10%, polyol ether 1-12%, terpene essential oil 1-5%, phospholipid and / or phospholipid derivative 0.5-10%, flexible agent 0.5-16%, and honokiol 0.1-2%, cinnamic acid 0.1-2%, polyol 1-15%, additive 0-25%, and the balance is water.

[0011] The polyol includes one or more of glycerol, ethanol, dipropylene glycol, polyethylene glycol, propylene glycol, and butylene glycol.

[0012] Further, the polyol ether includes one or more of myristyl ether, diethylene glycol monoethyl ether, isosorbide dimethyl ether, PEG / PPG / dimethyl ether, and ethylene glycol monobenzyl ether.

[0013] The terpene essential oil includes one or more of melaleuca alternifolia oil, basil essential oil, thyme essential oil, bergamot essential oil, mint essential oil, lemon essential oil, angelica essential oil, clary sage essential oil, chamomile essential oil, cypress essential oil, lavender essential oil, cinnamon essential oil, lemon grass essential oil, cedar essential oil, rosemary essential oil, and eucalyptus leaf oil.

[0014] The phospholipid includes one or more of soybean lecithin and hydrogenated lecithin.

[0015] The phospholipid derivative includes one or more of distearoyl phosphatidylcholine, dioleoyl lecithin, dimyristoyl lecithin, 1-palmitoyl-2-oleoyl lecithin, dipalmitoyl lecithin, distearoyl phosphatidyl ethanolamine, dioleoyl phosphatidyl ethanolamine, 1,2-palmitoyl phosphatidyl glycerol, and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000.

[0016] The flexibility agent includes one or more of sodium cholate, PEG-40 hydrogenated castor oil, PEG castor oil, laureth, glucoside, polyethylene glycol, polyglycerol ester, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, cetearyl alcohol, and glyceryl stearate.

[0017] Further, the PEG castor oil includes one or more of PEG-30 castor oil, PEG-35 castor oil, PEG-40 castor oil.

[0018] Further, the laureth includes one or more of laureth-7, laureth-9, laureth-12, laureth-23, and sodium laureth sulfate.

[0019] Further, the glucoside includes one or more of cocoglycoside, decyl glucoside, octyl decyl glucoside, and lauryl glucoside.

[0020] Cinnamic acid is a lipophilic unsaturated carboxylic acid with a faint floral scent, and is a natural antioxidant and anti-aging agent. The scalp hair follicle cells are damaged by the external environment, causing cell apoptosis, thereby causing the hair rooted in the hair follicle to fall off. Therefore, supplementing cinnamic acid to the damaged hair follicle cells can effectively accelerate the healing of the damaged hair follicle cells and achieve the effect of stabilizing the hair. In the present application, cinnamic acid also plays a role in enhancing the stability of pterostilbene. Cinnamic acid and pterostilbene are both phenylpropene skeletons, and cinnamic acid can protect the double bond and hydroxyl group of pterostilbene from oxidation. In addition, cinnamic acid is commonly found in cinnamon oil, basil essential oil, and Peruvian balsam essential oil, and therefore has excellent compatibility with essential oils and plays a crucial role in enhancing the antioxidant capacity of pterostilbene, essential oils, and phospholipids.

[0021] Honokiol is an active ingredient extracted from Magnolia bark and belongs to natural bisphenols, which has significant antioxidant, anti-allergic, and anti-inflammatory activities, can scavenge free radicals and reduce inflammatory reactions, and also has anti-ultraviolet activity, and can synergistically protect pterostilbene and terpene essential oil components sensitive to ultraviolet light in the composition with cinnamic acid. In addition, it can significantly down-regulate transforming growth factor-beta 1 (TGF-β1) that enhances androgen receptor affinity, and thus can prevent hair loss.

[0022] Further, the following components are also included: 3β-HSD inhibitor 0.1-10%, 17β-HSD inhibitor 0.1-7%.

[0023] Further, the 3β-HSD inhibitor is an inhibitor of type 1 3β-HSD, and type 2 isozyme is not expressed in the skin.

[0024] The 17β-HSD inhibitor is an inhibitor of type 3 and / or type 5 17β-HSD, both of which are expressed in the skin, hair follicle.

[0025] Further, the 3β-HSD inhibitor or 17β-HSD inhibitor includes one or more of flavonoids, isoflavonoids, polyphenols, terpenoids, phytosterols, fatty acids, plant extracts in which at least one of them is a main active ingredient, which have very low bioavailability in the body and are safe when used in the form of external use to the skin.

[0026] Further, the 3β-HSD inhibitor includes one or more of quercetin, resveratrol, pinoresinol, chalcone.

[0027] Further, the 17β-HSD inhibitor includes one or more of chrysin, baicalein, 7-hydroxyflavone, pueraria extract, ginkgo biloba extract.

[0028] Further, it further includes a 5α-reductase inhibitor in an amount of 0.2 to 8% by mass.

[0029] Further, the 5α-reductase inhibitor includes one or more of flavonoids, isoflavonoids, polyphenols, terpenoids, phytosterols, fatty acids, plant extracts in which at least one of them is a main active ingredient.

[0030] Further, the 5α-reductase inhibitor includes one or more of myricetin, kaempferol, liquiritigenin, retinol, curcumin, genistein, formononetin, stigmasterol, tocol, β-sitosterol, phytosterol, tea polyphenol, epigallocatechin gallate (EGCG), thujone, anthocyanin, daidzein, dihydroquercetin, safflower yellow, saw palmetto extract, azelaic acid, zinc, luteolin, rutin, fisetin, emodin, sphingosine, oleic acid, linoleic acid, linolenic acid, lauric acid, myristic acid, tocol, ganoderma extract, dutasteride, finasteride.

[0031] Further, the additive includes one or more of an androgen receptor antagonist, a hair loss prevention agent, a bacteriostatic anti-inflammatory agent, a thickening agent, a moisturizing agent, a preservative, a pH adjuster, a protein synthesis promoter, a chelating agent, a stabilizer, an antioxidant, an anti-allergic agent, a surfactant, an anti-dandruff agent, a whitening agent, an anti-wrinkle agent, a liquid lipid material.

[0032] Further, the androgen receptor antagonist includes one or more of ellagic acid, coumestrol, coumarin, baicalin.

[0033] Further, the anti-alopecia agent includes one or more of diaminopyrimidine oxide, radix polygoni multiflori praeparata extract, safflower extract, pyrrolidinyl diaminopyrimidine oxide, biota orientalis extract, adenosine, dianthus superbus extract, caffeine, isoquercitrin, hesperidin, hesperidin methyl chalcone, cedrol, chalcone, biotin, urtica dioica extract, ginger extract, herba epimedii extract, coffee extract, rhinacanthus nasutus extract, pinus palustris extract, radix anemarrhenae extract, prunella vulgaris extract, semen cuscutae extract, pollen extract, olea europaea leaf extract, ligustrum lucidum extract, angelica sinensis extract, panax ginseng extract, minoxidil.

[0034] Further, the bacteriostatic anti-inflammatory agent includes one or more of prunus armeniaca extract, trifolium pratense extract, dictamnus dasycarpus extract, silybum marianum extract, silymarin, magnolol, paeonol, berberine, radix scutellariae extract, naringenin, glycyrrhizin, glycyrrhetinic acid, sophora flavescens extract, aralia elata extract.

[0035] Further, the thickening agent includes one or more of carbomer, cellulose, modified starch, xanthan gum, dimethicone, cetyl alcohol, poloxamer, polyquaternium-10, polyacrylic acid, acrylate copolymer.

[0036] Further, the humectant includes one or more of sodium hyaluronate, ceramide, allantoin, dipotassium glycyrrhizate, ethylhexylglycerin, aloe vera gel, sodium lactate.

[0037] Further, the preservative includes one or more of phenoxyethanol, benzoic acid, parabens, p-hydroxyacetophenone, benzalkonium chloride, caprylyl oxyhydroxamic acid, and carboxin.

[0038] Further, the pH adjuster includes one or more of lactic acid, arginine, citric acid, malic acid, acetic acid, phosphoric acid, triethanolamine; and the pH value is 4-6.

[0039] Further, the protein synthesis promoter includes one or more of tripeptide-1 copper, acetyl tetrapeptide-3, palmitoyl tripeptide-1, oligopeptide, myristoyl pentapeptide-4, acetyl hexapeptide-1, acetyl hexapeptide-8, and collagen.

[0040] Further, the chelating agent includes one or more of disodium EDTA and tetrasodium EDTA.

[0041] Further, the stabilizer includes one or more of polyvinylpyrrolidone (PVP), hydroxypropyl methylcellulose (HPMC), sodium hydroxymethylcellulose (CMC), hydroxypropyl cyclodextrin, cyclodextrin, hydroxypropyl chitosan, carboxymethyl chitosan, alginic acid and its sodium salt.

[0042] Further, the whitening agent includes one or more of arbutin, resorcinol, kojic acid, tranexamic acid, azelaic acid, and nicotinamide.

[0043] Further, the anti-wrinkle agent includes one or more of vitamin A or its derivatives, vitamin C, recombinant collagen, and bakuchiol.

[0044] Further, the liquid lipid material includes one or more of acetylated monoglyceride, jojoba oil, pumpkin seed oil, isopropyl myristate, octyldodecanol, isopropyl palmitate, caprylic / capric triglyceride.

[0045] Further, the use of the nano-composition of pterostilbene in cosmetics or dermatology can further add solvents, gelling agents, softening agents, suspending agents, stabilizing agents, foaming agents, fragrances, ionic or non-ionic emulsifiers, fillers, sequestering agents, vitamins, barrier agents, humectants, dyes, pigments, hydrophilic or hydrophobic activators, lipid vesicles, elastase inhibitors, matrix metalloproteinase inhibitors, melanin synthesis stimulants or inhibitors, anti-wrinkle active agents, depigmenting agents, pro-pigmenting agents, self-tanning agents, anti-aging agents, inhibitors of NO synthase, inhibitors of lysyl and / or prolyl hydroxylase, antioxidants, alpha-hydroxy acids, free radical scavengers and / or anti-air pollution agents, anti-glycation agents, antihistamines, and the like. The above-mentioned aids are introduced in amounts commonly used in the cosmetics or dermatology field.

[0046] Further, the nano-composition of pterostilbene of the present application can employ a cosmetically or dermatologically acceptable skin delivery system selected from the group consisting of liposomes, oleosomes, nanocapsules, microneedles, cyclodextrins, vesicles, micelles, millispheres, microspheres, solutions, nanospheres, lipospheres, microemulsions, nanoemulsions, milliparticles, microparticles, nanoparticles.

[0047] Further, the nano-composition of pterostilbene of the present application can employ a cosmetically or dermatologically acceptable solid organic polymer or solid mineral matrix selected from the group consisting of talc, bentonite, silica, starch, maltodextrin.

[0048] Further, the cosmetically or dermatologically acceptable carrier of the nano-composition of pterostilbene of the present application comprises one or more emollients including, but not limited to, olive oil, squalene, soybean oil, dimethicone, glyceryl monoricinoleate, glyceryl monostearate, medium-chain triglycerides, isopropyl stearate, butyl stearate, lauryl lactate, octadecan-2-ol, caprylic triglyceride, capric triglyceride, panthenol, hydrogenated vegetable oil, tocopheryl acetate.

[0049] Further, the water is distilled water or purified water.

[0050] Further, the nano-composition of pterostilbene of the present application can be in various forms, including liposome, lipid nanovesicle, nanoparticle, nanoemulsion, microneedle, dressing, emulsion, gel, cream, lotion, hair tonic, mousse, powder, foam, spray, hair loss prevention serum, hair growth water, hair conditioner, scalp serum, shampoo, hair conditioner, pomade, hair cream, hair spray, hair dye, hair relaxer, hair gel, hair agent, mousse, hair fixative, eyebrow growth promoter, eyelash growth promoter, freckle cream, facial mask, toner, whitening cream, acne gel, massage cream, paste, jelly, essence, eye cream, eye serum, cleansing milk, cleansing water, skin care serum.

[0051] In another aspect, the present application provides a preparation method of the nano-composition of pterostilbene of any one of the above, comprising the following steps:

[0052] (1) homogeneously stirring pterostilbene, polyol ether, terpene essential oil, cinnamic acid, phospholipid and / or phospholipid derivative, under normal temperature conditions to obtain component A;

[0053] (2) mixing flexible agent, honokiol, additive, 10%-30% water (10%-30% of the total mass of water) and stirring under heating until completely dissolved to obtain component B;

[0054] (3) slowly adding component B to component A while stirring under normal temperature conditions, and then adding the remaining water to obtain a nanoscale dispersion solution;

[0055] (4) subjecting the nanoscale dispersion solution obtained in step (3) to high-pressure homogenization treatment to obtain the nano-composition of pterostilbene;

[0056] or (1) homogeneously stirring pterostilbene, polyol ether, terpene essential oil, cinnamic acid, phospholipid and / or phospholipid derivative, polyol, under normal temperature conditions to obtain component A;

[0057] (2) mixing flexible agent, honokiol, additive, 10%-30% water (10%-30% of the total mass of water) and stirring under heating until completely dissolved to obtain component B;

[0058] (3) slowly adding component B to component A while stirring under normal temperature conditions, and then adding the remaining water to obtain a nanoscale dispersion solution;

[0059] (4) subjecting the nanoscale dispersion solution obtained in step (3) to high-pressure homogenization treatment to obtain the nano-composition of pterostilbene.

[0060] Further, the rotation speed of the homogenizing stirring in step (1) is 3000-6000 rpm, and the time of the homogenizing stirring is 5-8 min.

[0061] The temperature of the heating in step (2) is 40-70℃, and the stirring speed is 2000-6000rpm;

[0062] The pressure of the high-pressure homogenization in step (4) is 200-500bar, and the number of homogenization is 1-4.

[0063] In another aspect, the present application provides a use of the nano-composition of pterostilbene or pterostilbene in any of the above aspects for the preparation of a product for preventing or improving androgen-dependent skin problems.

[0064] Further, the nano-composition of pterostilbene or pterostilbene in any of the above aspects inhibits 5α-reductase activity.

[0065] In another aspect, the present application provides a use of pterostilbene for the preparation of a cosmetic or dermatological product for preventing or improving hair loss, preventing hair thinning caused by an increase in androgen in the skin, hirsutism, sebaceous gland hyperplasia.

[0066] In another aspect, the present application provides a use of the nano-composition of pterostilbene in any of the above aspects for the preparation of a cosmetic or dermatological product for preventing hair loss or improving, improving hair thinning, white hair, promoting hair growth, preventing hirsutism, sebaceous gland hyperplasia, improving skin aging, pigmentation, dullness, wrinkles, seborrheic dermatitis, acne, skin oil, whitening, inhibiting melanin production, preventing skin physical barrier damage.

[0067] Further, the nano-composition of pterostilbene can be used alone as a product or added to other cosmetic or dermatological formulations as a raw material composition.

[0068] The nano-composition of pterostilbene of the present application is topically applied by being applied to the skin or hair of a subject, and is not limited to a specific subject.

[0069] The nano-composition of pterostilbene of the present application can be used in a form applied to the skin, and can also be used in a form applied using a microneedle or the like to facilitate skin absorption.

[0070] Compared to the prior art, the present application has the following beneficial effects:

[0071] 1) The nano-composition of pterostilbene of the present application has a suitable particle size of 10-200nm, and this particle size ensures an increase in skin absorption and skin retention of pterostilbene.

[0072] 2) The nano composition of the invention can significantly improve the loading capacity of pterostilbene, and the concentration of pterostilbene can reach more than 10%. The nano composition of pterostilbene can act as a reservoir to provide controlled and sustained release effects, maintain an effective concentration for a longer period of time, and better exert active effects. Compared with traditional liposomes, less solvent can be used to achieve a higher concentration, and the skin feel, effectiveness and stability of the product are improved, solving the problem of insufficient application of pterostilbene.

[0073] 3) The nano composition of the invention has good stability: first, the isosorbide dimethyl ether contained in the formula has the same double benzene ring and dimethyl ether structure as pterostilbene, has high compatibility, and can enhance the stability of pterostilbene molecules; second, the terpenoid essential oil, honokiol and cinnamic acid components in the formula have excellent antioxidant activity, which can prevent pterostilbene and phospholipids from being oxidized and discolored, and improve the stability of pterostilbene; at the same time, the bilayer structure of phospholipids can prevent the volatilization of essential oils. The nano composition of pterostilbene of the invention has good stability after being placed at 40℃ for 3 months.

[0074] 4) The use of terpenoid essential oil instead of liquid lipid as the main inner core lipophilic phase can avoid the shortcomings of traditional liposomes using liquid lipids, and can also enhance solubility. At the same time, some essential oils (such as eucalyptus oil and peppermint oil) are natural penetration enhancers, which can provide higher solubility and skin absorption capacity of pterostilbene, and can prevent oxidation and discoloration of pterostilbene when used with other active substances in the composition; the hydrophobic components of terpenoid essential oils have smaller molecular structures than the triglycerides and free fatty acids of liquid lipids, and can synergistically change the fluidity of the lipid bilayer with polyol ethers, providing smaller particle sizes and better absorption.

[0075] 5) The nano composition of pterostilbene of the invention is mild and non-irritating to the scalp, has high safety, and the preferred essential oils and cinnamic acid have preservative and fragrance enhancing effects, so there is no need to add a large amount of alcohol solvent, preservative and fragrance, and the product has a good experience.

[0076] 6) The nano composition of pterostilbene of the invention can target five different hair loss prevention and hair growth promotion targets of androgenic alopecia, namely inhibiting 5α-reductase activity, inhibiting 3β-HSD and 17β-HSD activity, antagonizing androgen receptor expression, and improving scalp microcirculation, and the active ingredients for preventing hair loss in the nano composition can directly reach the target in the hair follicle, and can be released and controlled, significantly improving the bioavailability and enhancing the effect of preventing hair loss and promoting hair growth.

[0077] 7) The present application proves that pterostilbene has excellent anti-hair loss effect, and has strong 5α-reductase inhibitory activity itself through in vitro 5α-reductase inhibition test, and the nano composition containing pterostilbene, honokiol and cinnamic acid can synergistically enhance the inhibitory effect of pterostilbene on 5α-reductase and the anti-hair loss effect, while the nano composition enhances the solubility and stability of pterostilbene, and expands the application of pterostilbene.

[0078] 8) The preparation method of the present application is simple, and the components in the formula can be self-emulsified to form a nano dispersion after dissolution, which is suitable for industrialization and large-scale production. The preparation method does not need to heat pterostilbene, phospholipid and essential oil, and the addition of cinnamic acid enhances the antioxidant capacity, thereby ensuring the stability of the composition.

[0079] 9) The nano composition of pterostilbene of the present application is easily dissolved in water, alcohol or oily system, and can be directly added to different types of anti-hair loss and hair growth products, so that the cosmetic has the advantages of anti-hair loss, small irritation and convenient use.

[0080] 10) The nano composition of pterostilbene of the present application can be directly added to shampoo, hair conditioner and other products, or can be directly sprayed on the hair roots, which is convenient and diversified in use, and is suitable for large-scale promotion. BRIEF DESCRIPTION OF DRAWINGS

[0081] Figure 1 is a scanning electron microscope image of the nano composition of pterostilbene prepared in Example 6 of the present application;

[0082] Figure 2 is a morphology diagram of the solution prepared in Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 of the present application stored at 40°C for 90 days. DETAILED DESCRIPTION

[0083] In order to better understand the content of the present application, the content of the present application is further described below in combination with specific implementation methods, but the protection content of the present application is not limited to the following examples.

[0084] Example 1

[0085] The nano composition of pterostilbene comprises the following components with mass percentage: pterostilbene 2%, isosorbide dimethyl ether 6%, basil essential oil 3%, mint essential oil 0.6%, soybean lecithin 2%, laureth-9 2.5%, and purified water added to 100%.

[0086] Preparation of the nano composition of pterostilbene:

[0087] (1) Under normal temperature conditions, 6% isosorbide dimethyl ether, 3% basil essential oil, 0.6% peppermint essential oil, 2% pterostilbene, 2% soybean lecithin were added into the oil pot, and the homogenizing stirring speed was kept at 6000 rpm, and stirring was carried out for 5 min to obtain component A;

[0088] (2) 2.5% lauryl alcohol polyether-9 was added into the water pot, and then purified water with a total mass of 15% purified water was added, heated to 58°C, and the stirring speed was kept at 4800 rpm, and stirring was carried out for 12 min to completely dissolve to obtain component B;

[0089] (3) Under normal temperature conditions, component B was slowly added into component A system while stirring, and then the remaining purified water was added to obtain a nanoscale dispersion solution;

[0090] (4) The nanoscale dispersion solution was subjected to high-pressure homogenization treatment at a pressure of 300 bar for 2 cycles, and then cooled to room temperature to obtain a pterostilbene nanocomposite.

[0091] The particle size of the pterostilbene nanocomposite prepared in Example 1 was detected by a Brookhaven laser particle size analyzer, and the detection result was: the particle size was 10.8 nm.

[0092] Example 2

[0093] The pterostilbene nanocomposite comprises the following components with mass percentage: pterostilbene 2%, diethylene glycol monoethyl ether 2%, isosorbide dimethyl ether 3%, melaleuca alternifolia leaf oil 0.5%, rosemary essential oil 1.3%, hydrogenated lecithin 7.4%, PEG-40 castor oil 1.5%, honokiol 0.3%, and cinnamic acid 1.8%, and purified water is added to 100%.

[0094] Preparation of the pterostilbene nanocomposite:

[0095] (1) Under normal temperature conditions, 2% diethylene glycol monoethyl ether, 3% isosorbide dimethyl ether, 1.8% cinnamic acid, 0.5% melaleuca alternifolia leaf oil, 1.3% rosemary essential oil, 2% pterostilbene, and 7.4% hydrogenated lecithin were added into the oil pot, and the homogenizing stirring speed was kept at 4500 rpm, and stirring was carried out for 5 min to obtain component A;

[0096] (2) 1.5% PEG-40 castor oil, 0.3% honokiol were sequentially added into the water pot, and then purified water with a total mass of 30% purified water was added, heated to 58°C, and the stirring speed was kept at 4000 rpm, and stirring was carried out for 10 min to completely dissolve to obtain component B;

[0097] (3) Under normal temperature conditions, component B was slowly added into component A system while stirring, and then the remaining purified water was added to obtain a nanoscale dispersion solution;

[0098] (4) The nanoscale dispersion solution is subjected to high-pressure homogenization treatment at a pressure of 200 bar for 3 cycles, and cooled to room temperature to obtain the nano composition of pterostilbene.

[0099] The particle size of the nano composition of pterostilbene prepared in Example 2 is detected by a Brookhaven laser particle size analyzer, and the detection result is: the particle size is 12.9 nm.

[0100] Example 3

[0101] The nano composition of pterostilbene comprises the following components in mass percentage: pterostilbene 3%, isosorbide dimethyl ether 2%, melaleuca alternifolia leaf oil 0.6%, eucalyptus oil 1.2%, peppermint essential oil 0.3%, dipalmitoyl lecithin 2.4%, PEG-40 castor oil 1%, honokiol 0.2%, cinnamic acid 0.3%, butanediol 3.5%, resveratrol 0.8%, chrysin 0.5%, EGCG 0.5%, pyrrolidinyl diaminopyrimidine oxide 0.16%, lactic acid 0.6%, and purified water added to 100%.

[0102] Preparation of the nano composition of pterostilbene:

[0103] (1) Under normal temperature conditions, 2% isosorbide dimethyl ether, 0.6% melaleuca alternifolia leaf oil, 0.3% cinnamic acid, 1.2% eucalyptus essential oil, 0.3% peppermint essential oil, 3% pterostilbene, 2.4% dipalmitoyl phosphatidylcholine, and 3.5% butanediol are added to an oil pot, and the homogenizing stirring speed is kept at 6000 rpm, and stirring is performed for 5 min to obtain component A;

[0104] (2) 1% PEG-40 castor oil, 0.2% honokiol, 0.8% resveratrol, 0.5% chrysin, 0.5% EGCG, 0.16% pyrrolidinyl diaminopyrimidine oxide, and 0.6% lactic acid are sequentially added to a water pot, and then purified water with a total mass of 15% is added, heated to 58°C, and stirred at a speed of 5000 rpm for 10 min to completely dissolve to obtain component B;

[0105] (3) Under normal temperature conditions, component B is slowly added to the component A system while stirring, and then the remaining purified water is added to obtain a nanoscale dispersion solution;

[0106] (4) The nanoscale dispersion solution is subjected to high-pressure homogenization treatment at a pressure of 260 bar for 3 cycles, and cooled to room temperature to obtain the nano composition of pterostilbene.

[0107] The particle size of the nano composition of pterostilbene prepared in Example 3 is detected by a Brookhaven laser particle size analyzer, and the detection result is: the particle size is 16.2 nm.

[0108] Example 4

[0109] Nano composition of pterostilbene, including the following mass percentage of components: pterostilbene 5%, myristyl alcohol ether 0.5%, isosorbide dimethyl ether 2%, lemon grass essential oil 1.4%, mint essential oil 0.3%, soybean lecithin 4%, cocoglycoside 1%, honokiol 0.1%, cinnamic acid 0.4%, ethanol 2.6%, butanediol 3.5%, resveratrol 2%, chrysin 0.2%, EGCG 0.3%, quercetin 0.5%, phenoxyethanol 0.6%, ethylhexylglycerin 0.2%, dipotassium glycyrrhizinate 1%, and purified water to 100%.

[0110] Preparation of nano composition of pterostilbene:

[0111] (1) Under normal temperature conditions, add 0.5% myristyl alcohol ether, 2.6% ethanol, 2% isosorbide dimethyl ether, 1.4% lemon grass essential oil, 0.4% cinnamic acid, 0.3% mint essential oil, 5% pterostilbene, 4% soybean lecithin, and 3.5% butanediol into the oil pot, and homogenously stir at a speed of 4000 rpm for 5 min to obtain component A;

[0112] (2) Add 1% cocoglycoside, 0.1% honokiol, 2% resveratrol, 0.2% chrysin, 0.3% EGCG, 0.5% quercetin, 0.6% phenoxyethanol, 0.2% ethylhexylglycerin, and 1% dipotassium glycyrrhizinate into the water pot in sequence, and then add purified water with a total mass of 18% purified water, heat to 68°C, and stir at a speed of 5000 rpm for 8 min to completely dissolve to obtain component B;

[0113] (3) Under normal temperature conditions, slowly add component B into component A system while stirring, and then add the remaining purified water to obtain a nanoscale dispersion solution;

[0114] (4) Subject the nanoscale dispersion solution to high-pressure homogenization treatment at a pressure of 300 bar for 2 cycles, and cool to room temperature to obtain the nano composition of pterostilbene.

[0115] The particle size of the nano composition of pterostilbene prepared in Example 4 was detected by a Brookhaven laser particle size analyzer, and the detection result was: the particle size was 20.7 nm.

[0116] Example 5

[0117] Nano composition of pterostilbene, comprising the following components by mass percentage: pterostilbene 10%, diethylene glycol monoethyl ether 3.5%, isosorbide dimethyl ether 5%, basil essential oil 2.4%, cedar essential oil 0.2%, hydrogenated lecithin 6%, PEG-40 hydrogenated castor oil 2%, laureth-23 0.5%, and honokiol 0.1%, cinnamic acid 0.6%, butanediol 2.5%, pinoresinol 0.3%, quercetin 0.4%, ginkgo leaf extract 0.1%, EGCG 0.5%, pyrrolidinyl diaminopyrimidine oxide 0.1%, phenoxyethanol 0.5%, and purified water added to 100%.

[0118] Preparation of nano composition of pterostilbene:

[0119] (1) Under normal temperature conditions, add 3.5% diethylene glycol monoethyl ether, 5% isosorbide dimethyl ether, 0.6% cinnamic acid, 2.4% basil essential oil, 0.2% cedar essential oil, 10% pterostilbene, 6% hydrogenated lecithin, and 2.5% butanediol to the oil pot, and homogenously stir at a speed of 7000 rpm for 6 min to obtain component A;

[0120] (2) Add 2% PEG-40 hydrogenated castor oil, 0.5% laureth-23, 0.1% honokiol, 0.3% pinoresinol, 0.1% ginkgo leaf extract, 0.5% EGCG, 0.4% quercetin, 0.1% pyrrolidinyl diaminopyrimidine oxide, and 0.5% phenoxyethanol to the water pot in sequence, and then add purified water with a total mass of 20% of purified water, heat to 70°C, and stir at a speed of 5000 rpm for 10 min to completely dissolve to obtain component B;

[0121] (3) Under normal temperature conditions, slowly add component B to component A system while stirring, and then add the remaining purified water to obtain a nanoscale dispersion solution;

[0122] (4) Subject the nanoscale dispersion solution to high-pressure homogenization treatment at a pressure of 300 bar for 2 cycles, and cool to room temperature to obtain the nano composition of pterostilbene.

[0123] The particle size of the nano composition of pterostilbene prepared in Example 5 was detected by a Brookhaven laser particle size analyzer, and the detection result was: the particle size was 44.7 nm.

[0124] Example 6

[0125] A nano composition of pterostilbene, comprising the following components in mass percentage: pterostilbene 5%, isosorbide dimethyl ether 2%, peppermint essential oil 1.4%, cedar essential oil 0.2%, soybean lecithin 3.6%, laureth-9 1%, honokiol 0.1%, cinnamic acid 0.6%, butanediol 2.5%, jojoba oil 1%, sodium cholate 0.5%, resveratrol 0.3%, pycnogenol 0.6%, ginkgo biloba extract 0.1%, chrysin 1.2%, baicalein 0.1%, pyrrolidinyl diaminopyrimidine oxide 0.1%, red clover extract 1%, lactic acid 1%, arginine 1.3%, phenoxyethanol 0.5%, and purified water added to 100%.

[0126] Preparation of a nano composition of pterostilbene:

[0127] (1) Under normal temperature conditions, 2% isosorbide dimethyl ether, 1.4% peppermint essential oil, 0.6% cinnamic acid, 0.2% cedar essential oil, 3% pterostilbene, 3.6% soybean lecithin, and 2.5% butanediol were added to an oil pot, and the homogenizing stirring speed was kept at 6000 rpm for 6 min to obtain component A;

[0128] (2) 0.5% sodium cholate, 1% laureth-9, 0.1% honokiol, 1% jojoba oil, 0.3% resveratrol, 0.1% ginkgo biloba extract, 0.6% pycnogenol, 1.2% chrysin, 0.1% pyrrolidinyl diaminopyrimidine oxide, 1% red clover extract, 0.1% baicalein, 1% lactic acid, 1.3% arginine, and 0.5% phenoxyethanol were sequentially added to a water pot, and then purified water with a total mass of 20% was added, heated to 70°C, and stirred at a speed of 5000 rpm for 6 min to completely dissolve to obtain component B;

[0129] (3) Under normal temperature conditions, component B was slowly added to the component A system while stirring, and then the remaining purified water was added to obtain a nano-level dispersion solution;

[0130] (4) The nano-level dispersion solution was subjected to high-pressure homogenization treatment at a pressure of 300 bar for 2 cycles, and then cooled to room temperature to obtain the nano composition of pterostilbene.

[0131] The particle size of the nano composition of pterostilbene prepared in Example 6 was detected by a Brookhaven laser particle size analyzer, and the detection result was: the particle size was 134.6 nm.

[0132] Example 7

[0133] A nano composition of pterostilbene, comprising the following components in mass percentage: pterostilbene 20%, isosorbide dimethyl ether 12%, ethylene glycol monobenzyl ether 6%, thyme essential oil 2.8%, eucalyptus oil 2.2%, happy clove essential oil 0.6%, hydrogenated lecithin 10%, dipalmitoyl lecithin 6%, PEG-40 hydrogenated castor oil 4.5%, octyldecyl glucoside 5.5%, honokiol 0.4%, cinnamic acid 1.6%, carnauba 0.12%, isopropyl palmitate 1.8%, resveratrol 0.3%, disodium EDTA 0.1%, penta-hydroxy flavone 0.1%, baicalein 0.1%, cypress leaf extract 0.8%, tea polyphenol 1.2%, swertia herb extract 0.4%, azelaic acid 0.15%, diaminopyrimidine oxide 0.3%, hydroxypropyl cyclodextrin 2%, phenoxyethanol 0.5%, acetyl tetrapeptide-3 0.1%, and purified water to 100%.

[0134] Preparation of a nano composition of pterostilbene:

[0135] (1) Under normal temperature conditions, add 20% pterostilbene, 12% isosorbide dimethyl ether, 6% ethylene glycol monobenzyl ether, 2.8% thyme essential oil, 2.2% eucalyptus oil, 0.6% happy clove essential oil, 10% hydrogenated lecithin, 6% dipalmitoyl lecithin, and 1.6% cinnamic acid to an oil pan, and homogenously stir at a speed of 6000 rpm for 6 min to obtain component A;

[0136] (2) Add 4.5% PEG-40 hydrogenated castor oil, 5.5% octyldecyl glucoside, 0.4% honokiol, 0.12% carnauba, 1.8% isopropyl palmitate, 0.15% azelaic acid, 0.3% diaminopyrimidine oxide, 0.3% resveratrol, 0.1% disodium EDTA, 0.1% penta-hydroxy flavone, 0.1% baicalein, 0.8% cypress leaf extract, 1.2% tea polyphenol, 0.4% swertia herb extract, 2% hydroxypropyl cyclodextrin, 0.5% phenoxyethanol, and 0.1% acetyl tetrapeptide-3 to a water pan in sequence, and then add purified water with a total mass of 15% purified water, heat to 70°C, and stir at a speed of 6000 rpm for 10 min to completely dissolve to obtain component B;

[0137] (3) Under normal temperature conditions, slowly add component B to the component A system while stirring, and then add the remaining purified water to obtain a nano-level dispersion solution;

[0138] (4) Subject the nano-level dispersion solution to high-pressure homogenization treatment at a pressure of 400 bar for 3 cycles, and cool to room temperature to obtain a nano composition of pterostilbene.

[0139] The particle size of the pterostilbene nanocomposite prepared in Example 7 was measured using a Brookhaven laser particle size analyzer. The result showed that the particle size was 187.7 nm.

[0140] Example 8

[0141] The nanocomposition of pterostilbene comprises the following components in weight percentage: 2% pterostilbene, 2% diethylene glycol monoethyl ether, 3% isosorbide dimethyl ether, 1% propylene glycol, 0.5% Melaleuca alternifolia leaf oil, 1.3% rosemary essential oil, 7.4% hydrogenated lecithin, 1.5% PEG-40 castor oil, 0.3% magnolol, 1.8% cinnamic acid, and purified water to 100%.

[0142] Preparation of nanocomposites of pterostilbene:

[0143] (1) Under normal temperature conditions, add 2% diethylene glycol monoethyl ether, 3% isosorbide dimethyl ether, 1% propylene glycol, 1.8% cinnamic acid, 0.5% Melaleuca alternifolia leaf oil, 1.3% rosemary essential oil, 2% pterostilbene and 7.4% hydrogenated lecithin to an oil pan, maintain the homogenization stirring speed at 4500 rpm, and stir for 5 min to obtain component A;

[0144] (2) Add 1.5% PEG-40 castor oil, 0.3% magnolol and then add purified water of 30% of the total mass of purified water to the water pot in sequence, heat to 58°C, keep the stirring speed at 4000 rpm, stir for 10 min, and completely dissolve to obtain component B;

[0145] (3) Under normal temperature conditions, while stirring, component B is slowly added to the component A system, and then the remaining purified water is added to obtain a nanoscale dispersion solution.

[0146] (4) The nano-scale dispersion solution is subjected to high-pressure homogenization at a pressure of 200 bar, 3 times, and then cooled to room temperature to obtain the nano-composite of pterostilbene.

[0147] The particle size of the pterostilbene nanocomposite prepared in Example 8 was measured using a Brookhaven laser particle size analyzer. The result showed that the particle size was 22.5 nm.

[0148] Comparative Example 1

[0149] Dissolve 2% pterostilbene in 30% ethanol and 30% glycerol, then add water to bring the solution to 100%. All percentages are by mass.

[0150] Comparative Example 2

[0151] Basil essential oil, peppermint essential oil and isosorbide dimethyl ether were not added, and the remaining components and preparation method were the same as in Example 1.

[0152] Comparative Example 3

[0153] Dissolve 2% pterostilbene, 0.3% magnolol, 1.5% cinnamic acid in 30% ethanol, 30% glycerol, and then add water to 100%. The percentages are all mass percentages.

[0154] Comparative Example 4

[0155] No pterostilbene was added, and the remaining components and preparation method were the same as in Example 2.

[0156] Test 1: solubility test of pterostilbene in terpene essential oils, commonly used liquid lipids, and polyhydric alcohol ethers

[0157] An excess of pterostilbene (recorded mass m0) was added to 10 mL of solvent, and constant temperature (25 ± 0.5°C) stirring was performed for 2 hours or more until dissolution equilibrium was reached; the saturated solution was quickly filtered using a funnel and filter paper, and after discarding the first 5 mL of filtrate, the filtrate was collected, and the unsolved solids were transferred together with the filter paper to a pre-weighed watch glass, which was dried to constant weight and then weighed (recorded mass m1); the solubility (mg / mL of solvent) was calculated according to the formula: solubility (mg / mL of solvent) = (m0-m1) / solvent volume, and the average value was taken from three parallel tests.

[0158] Ethyl oleate 47.6 mg / mL, castor oil 34.4 mg / mL, olive oil 35.8 mg / mL, melaleuca alternifolia oil 576.5 mg / mL, eucalyptus oil 953.6 mg / mL, basil essential oil 583.4 mg / mL, rosemary essential oil 374.0 mg / mL, isosorbide dimethyl ether 824.8 mg / mL, isosorbide dimethyl ether + basil essential oil (volume ratio 3:1) 987.7 mg / mL.

[0159] Test 2: morphological characterization

[0160] The morphology of the pterostilbene nanocomposition prepared in Example 6 was characterized by scanning electron microscopy (SEM), and the results are shown in Figure 1 The results show that the morphology of the pterostilbene liposome prepared in Example 6 is spherical and uniform in size.

[0161] Test 3: stability test

[0162] 1) Effect of centrifugal acceleration test on the stability of pterostilbene nanocomposition

[0163] The pterostilbene nanocompositions prepared in Examples 1, 2, 3, 4, 5, 6, and 7 were centrifuged at 20,000 r / min, and after 15 min, the appearance of the nanocomposition remained clear and transparent, and no oil-water layering phenomenon occurred.

[0164] 2) Sample observation test

[0165] The solutions prepared in Example 1 and Comparative Examples 1, 2, and 3 were placed in sealed containers at 40°C for 90 days. The morphology of the samples was then examined for any aggregation or discoloration. The morphological images of the solutions prepared in Example 1, Comparative Examples 1, 2, and 3 after 90 days of storage at 40°C are shown below. Figure 2 As shown in the figures, the 2% pterostilbene solution prepared in Comparative Example 1 was initially colorless, turning light yellow after the second day, and gradually deepening in color over time, reaching a brownish-yellow color after 90 days without any aggregation. Comparative Example 3, based on Comparative Example 1, added magnolol and cinnamic acid; its 2% pterostilbene solution showed no significant color change after 90 days, remaining colorless. The nanocomposite prepared in Comparative Example 2 showed a significant color deepening after 90 days without aggregation. In contrast, the nanocomposite prepared in Example 1 showed a slight color deepening after 90 days without aggregation. This indicates that the addition of terpene essential oils, polyol ethers, magnolol, and cinnamic acid can further enhance the stability of pterostilbene.

[0166] Test 4: Anti-hair loss effect test

[0167] Experimental setup: Seven experimental groups were set up, with 20 test subjects in each group (including 10 men and 10 women), aged between 20 and 55 years old. Among them, the male test subjects in each group had hair loss grades II-IV, and the female test subjects had hair loss grades I or II.

[0168] Male pattern baldness can be classified into 7 types: Type I: Normal or near-normal hairline; Type II: The hairline in the frontotemporal region recedes in a triangular pattern, reaching up to 2 cm from the coronal line connecting the two ear canals; Type III: The hairline in both temples recedes beyond 2 cm in front of the coronal line, and the top of the head appears sparse; Type IV: The hairline in both temples recedes significantly, accompanied by a receding hairline in the middle of the forehead, but separated by a moderately dense band of hair; Type V: The bald area in the frontotemporal and top regions expands, with a narrow and sparse band of hair in between; Type VI: Horseshoe-shaped baldness, with increased baldness on the sides and back; Type VII: Severe form, with baldness around the ears and the occipital region in addition to the horseshoe-shaped baldness.

[0169] Female hair loss can be classified into three types: Type I (mild): The hair at the front of the crown is thinning, requiring a change in hairstyle to conceal it, but the hairline at the forehead remains intact; Type II (moderate): The thinning of the hair at the front of the crown is more obvious, the area of ​​hair loss expands, and it is difficult to conceal by changing hairstyle; Type III (severe): The top of the head is completely bald or bald, but the hairline at the forehead remains intact.

[0170] Test method: Volunteers washed their hair once a day for 3 consecutive days. During the shampoo, the hair was washed with running water over a water tank lined with a filter cloth. The hair loss on the filter cloth was collected and the average amount of hair loss per shampoo was calculated. Then the experiment began: 1 ml of the product was applied twice a day, morning and evening, for 8 consecutive weeks to 6 test groups: Examples 1, 2, 6 and Comparative Examples 1, 2, 4 and the blank group (using 30% ethanol, 30% glycerin, and 40% water, all percentages are by mass).

[0171] The product was applied primarily to the scalp and hair roots. After 8 weeks, the hair was shampooed once daily for 3 consecutive days, rinsed under running water over a sink lined with a filter cloth. Hair loss was collected from the filter cloth during the three shampooing sessions, and the average amount of hair lost per shampoo was calculated. The test results are shown in the table below:

[0172]

[0173] As can be seen from the table, after using Comparative Example 1 of this application, the average amount of hair loss in the test subjects decreased significantly compared with before use (week 0). Compared with Comparative Example 2, which lacked the polyol ethers and two essential oils, Example 1 showed improved hair loss prevention and hair care effects. The combination of Example 2 with magnolol, cinnamic acid, and the plant-derived inhibitors of 3β-HSD, 17β-HSD, and 5α-reductase from Example 6 further reduced the amount of hair loss after washing, proving that the pterostilbene and related hair loss prevention compositions disclosed in this invention can effectively prevent hair loss, and that the components of the hair loss prevention and hair care compositions provided by this invention have a synergistic effect.

[0174] Test 5: Evaluation Experiment of 5α-Reductase Activity Inhibition Effect

[0175] A blank control group (using 30% ethanol, 30% glycerol, and 40% water, all percentages by mass) (negative experiment), a 2% pterostilbene solution group (Comparative Example 1), and a 2% pterostilbene nanocomposition group (Example 2) were prepared and sent to a third-party institution for testing. The androgen testosterone was converted to dihydrotestosterone by reducing coenzyme NADPH under the action of 5α-reductase. The concentration of reducing coenzyme NADPH was determined by ultraviolet spectrophotometry, and the change in the concentration of reducing coenzyme NADPH reflected the activity of 5α-reductase.

[0176] Weigh 1g of sample solution (blank control group, comparative example 1, and example 2), dilute to 10mL with Tris buffer, shake well to disperse, sonicate for 10min, centrifuge at 5000r / min for 5min, and use the supernatant for experiments; react the supernatant with 5α-reductase, testosterone, and reducing coenzyme, respectively, and detect the change in absorbance (ΔA = A) within 6min at 340nm. 6min –A 0min Finally, the inhibition rate of 5α-reductase activity was calculated.

[0177] 5α-reductase activity inhibition rate calculation formula: 5α-reductase activity inhibition rate (%) = (ΔA1-ΔA2) / ΔA1×100%, wherein: ΔA1 is the absorbance difference of negative experiment, and ΔA2 is the absorbance difference of sample experiment.

[0178] Experimental results:

[0179] The sample 5α-reductase enzyme activity inhibition rate test results are shown in the following table.

[0180] Group Inhibition rate of 5α-reductase activity (%) Blank control group 0 Comparative Example 1 70.40％ Example 2 78.42％

[0181] Compared with the blank control group, the comparative example 1 can significantly inhibit 5α-reductase, while the nano composition of the example 2 pterostilbene further increases the inhibition of 5α-reductase enzyme activity, which is related to the enhanced ability to penetrate the biological membrane and the synergy of the enzyme inhibition of other components (honokiol, cinnamic acid).

[0182] The above is only a specific embodiment of the present application, not all embodiments, any equivalent transformation of the technical solution of the present application by a person skilled in the art through reading the present application is covered by the claims of the present application.

Claims

1. A nano-composition of pterostilbene, characterized in that, comprise the following components in mass percentage: pterostilbene 0.1-25%, polyol ether 0.1-30%, terpene essential oil 0.1-8%, flexer 0.5-30%, phospholipid and / or phospholipid derivative 0.1-20%, honokiol 0-2%, cinnamic acid 0-3%, additive 0-35%, and the balance being water.

2. The nano-composition of pterostilbene as claimed in claim 1, wherein, comprise the following components in mass percentage: pterostilbene 0.1-10%, polyol ether 1-12%, terpene essential oil 1-5%, phospholipid and / or phospholipid derivative 0.5-10%, flexer 0.5-16%, honokiol 0.1-2%, cinnamic acid 0.1-2%, polyol 1-15%, additive 0-25%, and the balance being water; The polyol comprises one or more of glycerol, ethanol, dipropylene glycol, polyethylene glycol, propylene glycol, and butylene glycol.

3. The nano-composition of pterostilbene as claimed in claim 1 or 2, wherein, The polyol ether comprises one or more of myristyl ether, diethylene glycol monoethyl ether, isosorbide dimethyl ether, PEG / PPG / dimethyl ether, and ethylene glycol monobenzyl ether. The terpene essential oil comprises one or more of melaleuca alternifolia oil, basil essential oil, thyme essential oil, bergamot essential oil, mint essential oil, lemon essential oil, angelica essential oil, clary sage essential oil, chamomile essential oil, cypress essential oil, lavender essential oil, cinnamon essential oil, lemon grass essential oil, cedar essential oil, rosemary essential oil, and eucalyptus leaf oil. The phospholipid comprises one or more of soybean lecithin and hydrogenated lecithin. The phospholipid derivative comprises one or more of distearoyl phosphatidylcholine, dioleoyl lecithin, dimyristoyl lecithin, 1-palmitoyl-2-oleoyl lecithin, dipalmitoyl lecithin, distearoyl phosphatidyl ethanolamine, dioleoyl phosphatidyl ethanolamine, 1,2-palmitoyl phosphatidyl glycerol, and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000. The flexer comprises one or more of sodium cholate, PEG-40 hydrogenated castor oil, PEG castor oil, lauryl alcohol polyether, glucoside, polyethylene glycol, polyglycerol ester, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, cetyl stearyl alcohol, and glyceryl stearate. Preferably, the PEG castor oil comprises one or more of PEG-30 castor oil, PEG-35 castor oil, and PEG-40 castor oil. Preferably, the lauryl alcohol polyether comprises one or more of lauryl alcohol polyether-7, lauryl alcohol polyether-9, lauryl alcohol polyether-12, lauryl alcohol polyether-23, and lauryl alcohol polyether sodium sulfate. Preferably, the glucoside comprises one or more of cocoyl glucoside, decyl glucoside, octyldecyl glucoside, and lauryl glucoside.

4. The nano-composition of pterostilbene as claimed in claim 1 or 2, wherein, The following components in mass percentage are further included: 3β-HSD inhibitor 0.1-10%, 17β-HSD inhibitor 0.1-7%; Preferably, the 3β-HSD inhibitor comprises one or more of quercetin, resveratrol, pterosil, and chalcone. Preferably, the 17β-HSD inhibitor comprises one or more of chrysin, baicalein, 7-hydroxyflavone, pueraria extract, and ginkgo biloba leaf extract.

5. The nano-composition of pterostilbene as claimed in claim 4, wherein, Also included are components in the following mass percentages: 5α-reductase inhibitor 0.2-8%; Preferably, the 5α-reductase inhibitor includes one or more of myricetin, kaempferol, ligustrazine, riboflavin, curcumin, genistein, formononetin, stigmasterol, tocol, β-sitosterol, phytosterol, tea polyphenol, epigallocatechin gallate (EGCG), thujone, anthocyanin, daidzein, dihydroquercetin, safflower yellow pigment, saw palmetto extract, azelaic acid, zinc, luteolin, rutin, fisetin, emodin, sphingosine, oleic acid, linoleic acid, linolenic acid, lauric acid, myristic acid, toxirin A, ganoderma extract, dutasteride, finasteride.

6. The nano-composition of pterostilbene as claimed in claim 1 or 2, wherein, The additive includes one or more of androgen receptor antagonists, anti-hair loss agents, bacteriostatic anti-inflammatory agents, thickening agents, moisturizing agents, preservatives, pH regulators, protein synthesis promoters, chelating agents, stabilizers, antioxidants, anti-allergic agents, surfactants, anti-dandruff agents, whitening agents, anti-wrinkle agents, and liquid lipid materials; Preferably, the androgen receptor antagonist includes one or more of ellagic acid, coumestrol, coumarin, baicalin; Preferably, the anti-hair loss agent includes one or more of diaminopyrimidine oxide, radix polygoni multiflori extract, safflower extract, pyrrolidinyl diaminopyrimidine oxide, biota leaf extract, adenosine, dianthus extract, caffeine, isoquercitrin, hesperidin, hesperidin methyl chalcone, cedrol, chalcone, biotin, nettle extract, ginger extract, sasa alpina extract, coffee extract, rhinacanthus nasutus extract, pine extract, anemarrhena asphodeloides extract, selfheal extract, cuscuta extract, pollen extract, olea europaea leaf extract, ligustrum lucidum extract, angelica extract, panax ginseng extract, minoxidil; Preferably, the bacteriostatic anti-inflammatory agent includes one or more of prunus mume extract, trifolium pratense extract, dictamnus dasycarpus extract, silybum marianum extract, silymarin, magnolol, paeonol, berberine, scrophularia ningpoensis root extract, naringenin, glycyrrhizin, glycyrrhetinic acid, sophora flavescens extract, aralia decaisneana extract; Preferably, the thickening agent includes one or more of carbomer, cellulose, modified starch, xanthan gum, dimethicone, cetyl alcohol, poloxamer, polyquaternium Q-10, polyacrylic acid, acrylate copolymer; Preferably, the moisturizing agent includes one or more of sodium hyaluronate, ceramide, allantoin, dipotassium glycyrrhizate, ethylhexylglycerin, aloe vera gel, sodium lactate; Preferably, the preservative includes one or more of phenoxyethanol, benzoic acid, parabens, p-hydroxyacetophenone, benzalkonium chloride, caprylyl oxyhydroxamic acid, and carboxin; Preferably, the pH regulator includes one or more of lactic acid, arginine, citric acid, malic acid, acetic acid, phosphoric acid, triethanolamine; and the pH value is 4-6; Preferably, the protein synthesis promoter includes one or more of tripeptide-1 copper, acetyl tetrapeptide-3, palmitoyl tripeptide-1, oligopeptide, myristoyl pentapeptide-4, acetyl hexapeptide-1, acetyl hexapeptide-8, and collagen. Preferably, the chelating agent comprises one or more of disodium EDTA, tetrasodium EDTA; Preferably, the stabilizer comprises one or more of polyvinylpyrrolidone, hydroxypropyl methylcellulose, sodium hydroxymethylcellulose, hydroxypropyl cyclodextrin, cyclodextrin, hydroxypropyl chitosan, carboxymethyl chitosan, alginic acid and its sodium salt; Preferably, the whitening agent comprises one or more of arbutin, resorcinol, kojic acid, tranexamic acid, azelaic acid, nicotinamide; Preferably, the anti-wrinkle agent comprises one or more of vitamin A or its derivatives, vitamin C, recombinant collagen, bakuchiol; Preferably, the liquid lipid material comprises one or more of acetylated monoglyceride, jojoba oil, pumpkin seed oil, isopropyl myristate, octyldodecanol, isopropyl palmitate, caprylic / capric triglyceride.

7. A process for the preparation of nano-composition of pterostilbene as claimed in any one of claims 1 to 6, wherein the process comprises the steps of: The method comprises the following steps: (1) Under normal temperature conditions, homogeneously stir pterostilbene, polyol ether, terpenoid essential oil, cinnamic acid, phospholipid and / or phospholipid derivative to obtain component A; (2) Mix flexible agent, honokiol, additive and 10%-30% water, heat and stir until completely dissolved to obtain component B; (3) Under normal temperature conditions, slowly add component B to component A while stirring, then add the remaining water to obtain a nanoscale dispersion solution; (4) Subject the nanoscale dispersion solution obtained in step (3) to high-pressure homogenization treatment to obtain the pterostilbene nanocomposition; or (1) Under normal temperature conditions, homogeneously stir pterostilbene, polyol ether, terpenoid essential oil, cinnamic acid, phospholipid and / or phospholipid derivative, polyol to obtain component A; (2) Mix flexible agent, honokiol, additive and 10%-30% water, heat and stir until completely dissolved to obtain component B; (3) Under normal temperature conditions, slowly add component B to component A while stirring, then add the remaining water to obtain a nanoscale dispersion solution; (4) Subject the nanoscale dispersion solution obtained in step (3) to high-pressure homogenization treatment to obtain the pterostilbene nanocomposition; Preferably, the stirring speed in step (1) is 3000-6000 rpm, and the stirring time is 5-8 min; Preferably, the heating temperature in step (2) is 40-70℃, and the stirring speed is 2000-6000 rpm; Preferably, the pressure for high-pressure homogenization treatment in step (4) is 200-500 bar, and the number of homogenization is 1-4 times.

8. Use of the pterostilbene nanocomposition or pterostilbene in the preparation of a product for preventing or improving androgen-dependent skin problems according to any one of claims 1-6.

9. Use of pterostilbene in the preparation of a cosmetic or dermatological product for preventing or improving hair loss, preventing hair thinning caused by elevated androgens in the skin, hirsutism, and sebaceous gland hyperplasia.

10. Use of the nano-composition of pterostilbene according to any one of claims 1-6 in the preparation of a cosmetic or dermatological product for preventing or improving hair loss, improving hair thinning, promoting hair growth, preventing hirsutism, sebaceous gland hyperplasia, improving skin aging, pigmentation, dullness, wrinkles, seborrheic dermatitis, acne, skin oiliness, whitening, inhibiting melanin production, preventing physical barrier damage of the skin.

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