Composite capsule with synergistic stomach-nourishing and anti-inflammatory effects and preparation method thereof

Abstract

The invention discloses a composite capsule with synergistic stomach-nourishing and anti-inflammatory effects and a preparation method thereof, and relates to the technical field of functional foods, health care products and medicines. The lactic acid bacteria, the egg yolk globulin and the dandelion extract are used as main raw materials to prepare the composite capsule, the ratio of the raw materials is optimized, the three raw materials in the composite capsule generate a synergistic effect, triterpenoid components in the dandelion extract can inhibit helicobacter pylori, and the triterpenoid components, the lactic acid bacteria and the egg yolk globulin jointly repair gastrointestinal mucosa, so that the gastric mucosa is inhibited, and the gastric mucosa is prevented from being damaged. Gastrointestinal health is synergistically regulated, and resistance is enhanced; meanwhile, the dandelion extract and the egg yolk globulin serve as protective carriers, the survival rate of the lactic acid bacteria in gastric acid is increased, and experimental data shows that the survival rate is increased to 81% from 30%; compared with a single formula, the compound capsule can increase the survival rate of lactic acid bacteria and increase the bioavailability of the dandelion extract by enhancing the immune effect of egg yolk globulin.

Description

TECHNICAL FIELD

[0001] The present application relates to the functional food, health care product and pharmaceutical technical field, specifically to a kind of compound capsule with synergistic stomach nourishing and anti-inflammatory and preparation method thereof. BACKGROUND

[0002] In the current stomach nourishing product field, there are many problems to be solved. As for traditional probiotic preparations, common products such as lactic acid bacteria have a survival rate generally less than 30% due to the harsh challenge of gastric acid environment, which greatly limits their colonization effect in the intestinal tract and makes it difficult to fully play a role. At the same time, although the dandelion extract has certain anti-inflammatory and stomach nourishing effect, its bioavailability is not satisfactory, and the synergistic potential between it and other ingredients has not been fully tapped. In addition, egg yolk globulin was mainly used for immunoregulation in the past, but few studies have combined it with probiotics and dandelion plant extracts.

[0003] The existing stomach nourishing and anti-inflammatory products also have obvious defects, showing the characteristics of treating symptoms but not the root cause. Most products such as acid suppressants and antacids only focus on relieving symptoms such as stomach pain and acid reflux, but ignore the root cause of these problems, such as Helicobacter pylori infection, gastric mucosa damage and long-term unhealthy eating habits. And long-term dependence on these products can have a series of negative effects, such as possible inhibition of normal gastric acid secretion, thereby interfering with normal digestive function. For example, if proton pump inhibitors are used excessively, they can even cause atrophic gastritis. From a long-term perspective, some acid suppressants may develop resistance after a period of use, resulting in gradually declining efficacy; at the same time, over-reliance on stomach nourishing products can also mask the true condition and delay timely diagnosis of serious diseases such as gastric cancer and gastric ulcer perforation. In addition, there are great differences in the adaptability of different individuals to these products. What is particularly prominent is that there is still a gap in technology, and there is no compound preparation on the market that can simultaneously achieve the three effects of gastric acid protection, mucosa repair and immune enhancement, and there is no report on the combination of lactic acid bacteria, egg yolk globulin and dandelion extract. SUMMARY

[0004] The purpose of the present application is to provide a kind of compound capsule with synergistic stomach nourishing and anti-inflammatory and preparation method thereof, to solve the problems in the prior art.

[0005] In order to solve the above technical problems, the present application provides the following technical scheme: a kind of compound capsule with synergistic stomach nourishing and anti-inflammatory, the compound capsule includes: 1~30wt% of lactic acid bacteria, 1~20wt% of egg yolk globulin, 1~30wt% of dandelion extract and 1~80wt% of auxiliary materials.

[0006] Further, the lactic acid bacteria are Lactobacillus plantarum or Lactobacillus acidophilus.

[0007] Further, the IgY content of the egg yolk globulin is greater than or equal to 90%.

[0008] Further, the total triterpenoid content of the dandelion extract is greater than or equal to 40%.

[0009] Further, the excipients include fillers and lubricants.

[0010] Further, the fillers include oligofructose and malt dextrin.

[0011] Further, the oligofructose includes one or more of sucrose triose, sucrose tetraose and sucrose pentaose.

[0012] Further, the DE value of the malt dextrin is 16-18%.

[0013] Further, the lubricant includes micronized silica gel.

[0014] Further, the micronized silica gel is of a pharmaceutical grade and is purchased from Hangzhou Chengyuan Biotechnology Co., Ltd.

[0015] Further, the preparation method of the complex capsule with synergistic stomach-nourishing and anti-inflammatory effects includes the following steps: mixing lactic acid bacteria dry powder, egg yolk globulin and dandelion extract for 10 minutes, then adding oligofructose, malt dextrin and micronized silica gel, mixing in a mixing machine for 15 minutes, filling the mixed powder into a capsule shell using a capsule filling machine, bottling, labeling and packaging.

[0016] Further, the capsule has a filling capacity of 380-420 mg.

[0017] Further, the complex capsule can improve the survival rate of lactic acid bacteria in gastric acid from 30% to 81%.

[0018] Compared with the prior art, the beneficial effects achieved by the present application are:

[0019] The present application prepares a complex capsule by taking lactic acid bacteria, egg yolk globulin and dandelion extract as main raw materials, optimizes the ratio of raw materials, makes the three in the complex capsule produce synergistic effects, the triterpenoid components in the dandelion extract can inhibit gastric Helicobacter pylori, and the lactic acid bacteria and egg yolk globulin can repair the gastrointestinal mucosa and synergistically regulate the gastrointestinal health and enhance the resistance; meanwhile, the dandelion extract and egg yolk globulin serve as protective carriers to improve the survival rate of lactic acid bacteria in gastric acid, and experimental data show that the survival rate is improved from 30% to 81%; compared with single and compound capsules, the complex capsule can enhance the immune effect of egg yolk globulin, increase the survival rate of lactic acid bacteria and increase the bioavailability of dandelion extract. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0021] In order to more clearly illustrate the method provided by the present application, the following embodiments are described in detail. In the following embodiments, the test methods of various indexes of the composite capsule with synergistic stomach-nourishing and anti-inflammatory effects are as follows.

[0022] 1. Gastrointestinal tract protection effect and bioavailability of dandelion extract:

[0023] (1) Establishing a DSS enteritis model of rats

[0024] DSS was dissolved in distilled water to prepare a 3% (W / W) DSS aqueous solution, which replaced the drinking water and was freely drunk by Wistar rats (SPF level, male, body weight 180-200 g) for 7 days.

[0025] (2) Animal grouping and administration

[0026] After the 7-day adaptation period, the Wistar rats (SPF level, male) were weighed and then randomly divided into 11 groups, 10 rats in each group, namely, a normal group, a model group, an embodiment 1 group, an embodiment 2 group, an embodiment 3 group, an embodiment 4 group, an embodiment 5 group, an embodiment 6 group, a comparative example 1 group, a comparative example 2 group and a comparative example 3 group. The normal group was given normal drinking water and freely drunk; the model group, the embodiment 1 group, the comparative example 1 group, the comparative example 2 group and the comparative example 3 group were respectively given 3% DSS aqueous solution and freely drunk; at the same time, the embodiment 1 group, the comparative example 1 group, the comparative example 2 group and the comparative example 3 group were administered by gavage according to the dosage, once a day, one capsule each time; the model group and the normal group were gavaged with normal saline. Each group was normally fed.

[0027] 2 mL of blood was taken from the forelimb vein before and after the first administration, and the blood sampling points were as follows: before administration, 0.5 h, 1 h, 1.5 h, 2 h, 2.5 h, 3 h, 3.5 h, 4 h, 4.5 h, 5 h, 5.5 h, 6 h, 6.5 h, 7 h, 8 h, 9 h, 10 h, 12 h, 14 h, 16 h and 24 h after administration. After the Wistar rats were orally administered with the sample of the embodiment 1, the sample of the comparative example 1, the sample of the comparative example 2 and the sample of the comparative example 3 under the same dosage, the concentration level of the dandelion active ingredient in the plasma and the basic pharmacokinetic characteristics were determined, the differences of the main parameters Cmax, Tmax and AUClast were compared, and the bioavailability of the dandelion extract was evaluated.

[0028] After 7 days of administration, the histological changes in the colon of the rats were observed by hematoxylin staining, and scored, with the score representing the degree and extent of mucosal injury, and the higher the score, the higher the degree of injury, and the ulcer index of the stomach of the rats was observed.

[0029] 2. Survival rate of lactic acid bacteria

[0030] The capsules obtained in Examples 1 to 6 and Comparative Examples 1 to 3 were respectively added into simulated gastrointestinal fluid prepared according to the method of reference Minekus in an amount of 10 times the mass of the capsules, and the survival rate of lactic acid bacteria after a certain period of time was analyzed; the survival rate (%) = M1 / M0 x 100%, wherein: M1 represents the number of viable lactic acid bacteria after treatment, in units of cfu / mL; M0 represents the number of viable lactic acid bacteria before treatment, in units of cfu / mL.

[0031] Example 1

[0032] 1. Raw material ratio (400 mg per capsule):

[0033] Material name Amount per capsule Amount per batch Lactobacillus plantarum 50 mg 0.5 kg Egg yolk globulin 10 mg 0.1 kg Dandelion extract 40 mg 0.4 kg Fructo-oligosaccharide 150 mg 1.5 kg Malt dextrin 140 mg 1.4 kg Microfine silica gel 10 mg 0.1 kg Total 1 capsule 10,000 capsules

[0034] 2. Preparation process:

[0035] After mixing the lactic acid bacteria dry powder, egg yolk globulin and dandelion extract for 10 min, fructooligosaccharides, malt dextrin and micronized silica gel were added, and mixed in a mixer for 15 min. The mixed powder was filled into a capsule shell using a capsule filling machine, with a capsule loading capacity of 400 mg, bottled, labeled, and packaged.

[0036] Example 2

[0037] 1. Raw material ratio (400 mg per capsule):

[0038] Material name Amount per capsule Amount per batch Lactobacillus plantarum 80 mg 0.8 kg Egg yolk globulin 40 mg 0.4 kg Dandelion extract 80 mg 0.8 kg Fructo-oligosaccharide 100 mg 1.0 kg Malt dextrin 90 mg 0.9 kg Microfine silica gel 10 mg 0.1 kg Total 1 capsule 10,000 capsules

[0039] 2. Preparation process:

[0040] After mixing the lactic acid bacteria dry powder, egg yolk globulin and dandelion extract for 10 min, fructooligosaccharides, malt dextrin and micronized silica gel were added, and mixed in a mixer for 15 min. The mixed powder was filled into a capsule shell using a capsule filling machine, with a capsule loading capacity of 400 mg, bottled, labeled, and packaged.

[0041] Example 3

[0042] 1. Raw material ratio (400 mg per capsule):

[0043] Material name Amount per capsule Amount per batch Lactobacillus plantarum 70 mg 0.7 kg Egg yolk globulin 30 mg 0.3 kg Dandelion extract 60 mg 0.6 kg Fructo-oligosaccharide 120 mg 1.2 kg Malt dextrin 100 mg 1.0 kg Microfine silica gel 20 mg 0.2 kg Total 1 capsule 10,000 capsules

[0044] 2. Preparation process:

[0045] Mixing the dry powder of lactic acid bacteria, egg yolk globulin and extract of Taraxacum mongolicum for 10 minutes, then adding fructo-oligosaccharide, malt dextrin and micro-powder silica gel, putting into the mixing machine for 15 minutes, filling the mixed powder into the capsule shell using the capsule filling machine, the capsule loading capacity is 400 mg, bottling, labeling and packaging.

[0046] Example 4

[0047] 1. Raw material ratio (400 mg per capsule):

[0048] Material name Amount per capsule Amount per batch Lactobacillus plantarum 60 mg 0.6 kg Egg yolk globulin 20 mg 0.2 kg Dandelion extract 120 mg 1.2 kg Fructo-oligosaccharide 95 mg 0.95 kg Malt dextrin 90 mg 0.9 kg Microfine silica gel 15 mg 0.15 kg Total 1 capsule 10,000 capsules

[0049] 2. Preparation process:

[0050] Mixing the dry powder of lactic acid bacteria, egg yolk globulin and extract of Taraxacum mongolicum for 10 minutes, then adding fructo-oligosaccharide, malt dextrin and micro-powder silica gel, putting into the mixing machine for 15 minutes, filling the mixed powder into the capsule shell using the capsule filling machine, the capsule loading capacity is 400 mg, bottling, labeling and packaging.

[0051] Example 5

[0052] 1. Raw material ratio (400 mg per capsule):

[0053] Material name Amount per capsule Amount per batch Lactobacillus plantarum 50 mg 0.5 kg Egg yolk globulin 80 mg 0.8 kg Dandelion extract 70 mg 0.7 kg Fructo-oligosaccharide 100 mg 1.0 kg Malt dextrin 90 mg 0.9 kg Microfine silica gel 10 mg 0.1 kg Total 1 capsule 10,000 capsules

[0054] 2. Preparation process:

[0055] Mixing the dry powder of lactic acid bacteria, egg yolk globulin and extract of Taraxacum mongolicum for 10 minutes, then adding fructo-oligosaccharide, malt dextrin and micro-powder silica gel, putting into the mixing machine for 15 minutes, filling the mixed powder into the capsule shell using the capsule filling machine, the capsule loading capacity is 400 mg, bottling, labeling and packaging.

[0056] Example 6

[0057] 1. Raw material ratio (400 mg per capsule):

[0058] Material name Amount per capsule Amount per batch Lactobacillus plantarum 120 mg 1.2 kg Egg yolk globulin 60 mg 0.6 kg Dandelion extract 100 mg 0.1 kg Fructo-oligosaccharide 60 mg 0.6 kg Malt dextrin 40 mg 0.4 kg Microfine silica gel 20 mg 0.2 kg Total 1 capsule 10,000 capsules

[0059] 2. Preparation process:

[0060] Mixing the dry powder of lactic acid bacteria, egg yolk globulin and extract of Taraxacum mongolicum for 10 minutes, then adding fructo-oligosaccharide, malt dextrin and micro-powder silica gel, putting into the mixing machine for 15 minutes, filling the mixed powder into the capsule shell using the capsule filling machine, the capsule loading capacity is 400 mg, bottling, labeling and packaging.

[0061] Comparative Example 1

[0062] The difference between Comparative Example 1 and Example 1 is that no egg yolk globulin and dandelion extract is added in the capsule, and the amount of lactobacillus plantarum is 100 mg per capsule; the remaining steps are the same as those in Example 1.

[0063] Comparative Example 2

[0064] The difference between Comparative Example 2 and Example 1 is that no lactobacillus plantarum and dandelion extract is added in the capsule, and the amount of egg yolk globulin is 100 mg per capsule; the remaining steps are the same as those in Example 1.

[0065] Comparative Example 3

[0066] The difference between Comparative Example 3 and Example 1 is that no lactobacillus plantarum and egg yolk globulin is added in the capsule, and the amount of dandelion extract is 100 mg per capsule; the remaining steps are the same as those in Example 1.

[0067] Effect Example

[0068] The performance analysis results of the complex capsules with synergistic stomach-nourishing and anti-inflammatory effect of Examples 1 to 6 and Comparative Examples 1 to 3 of the present application are given in Table 1 below.

[0069] Table 1

[0070] Degree of mucosal damage Extent of mucosal damage Survival rate of lactic acid bacteria (%) Bioavailability of dandelion extract (%) Example 1 1 1 81% 42% Example 2 1 1 77% 38% Example 3 1 1 74% 40% Example 4 1 1 79% 34% Example 5 1 1 76% 38% Example 6 1 1 66% 39% Comparative Example 1 2 1 30% / Comparative Example 2 2 3 / / Comparative Example 3 2 3 / 16%

[0071] At the same time, by observing the normal group, the model group, the Example 1 group, the Comparative Example 1 group, the Comparative Example 2 group and the Comparative Example 3 group, it is found that the ulcer index of the Example 1 group is reduced by 68%, which is significantly better than that of the single component; the ulcer index of the Comparative Example 1 group is reduced by 35%, the Comparative Example 2 group is reduced by 26%, and the Comparative Example 3 group is reduced by 28%.

[0072] From the comparison of the experimental data of Examples 1 to 6 and Comparative Examples 1 to 3, it can be found that the complex capsules prepared by using lactobacillus, egg yolk globulin and dandelion extract as main raw materials optimize the ratio of raw materials, so that the three produce a synergistic effect in the complex capsules, the triterpenoid components in the dandelion extract can inhibit Helicobacter pylori, and together with lactobacillus and egg yolk globulin, they can repair the gastrointestinal mucosa and synergistically regulate the health of the gastrointestinal system and enhance the resistance; at the same time, the dandelion extract and egg yolk globulin act as protective carriers to improve the survival rate of lactobacillus in gastric acid, and the experimental data shows that the survival rate is increased from 30% to 81%; compared with single and complex capsules, the complex capsules can enhance the immune effect of egg yolk globulin, increase the survival rate of lactobacillus and increase the bioavailability of dandelion extract.

[0073] It will be apparent to those skilled in the art that the application is not limited to the details of the above-exemplified embodiments and that the present application can be implemented in other particular forms without departing from the spirit or essential characteristics of the present application. The embodiments should therefore be considered in all respects as illustrative and not restrictive, the scope of the application being indicated by the appended claims rather than by the above description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. No reference herein to any prior patents or applications shall be taken as an admission that the application is not entitled to antedate such prior patents or applications by virtue of prior application. Any reference to the use of a term in the singular herein shall be taken as an acknowledgment of the existence of the term in the plural and vice versa.

Claims

1. A complex capsule having synergistic stomach-friendly anti-inflammatory, characterized by, The complex capsule comprises 1-30 wt% of lactic acid bacteria, 1-20 wt% of egg yolk globulin, 1-30 wt% of dandelion extract and 1-80 wt% of auxiliary materials.

2. The stomach-friendly and anti-inflammatory synergistic compound capsule according to claim 1, wherein The lactic acid bacteria are Lactobacillus plantarum or Lactobacillus acidophilus.

3. The compound capsule with synergistic stomach-nourishing and anti-inflammatory effects according to claim 1, characterized in that, The IgY content of the egg yolk globulin is greater than or equal to 90%.

4. A compound capsule with synergistic stomach-nourishing and anti-inflammatory effects according to claim 1, characterized in that, The total triterpene content of the dandelion extract is greater than or equal to 40%.

5. A compound capsule with synergistic stomach-nourishing and anti-inflammatory effects according to claim 1, characterized in that, The auxiliary materials comprise fillers and lubricants.

6. A compound capsule with synergistic stomach-nourishing and anti-inflammatory effects according to claim 5, characterized in that, The fillers comprise fructooligosaccharides and malt dextrin.

7. A compound capsule with synergistic stomach-nourishing and anti-inflammatory effects according to claim 5, characterized in that, The lubricants comprise micro-powder silica gel.

8. The method of claim 1, wherein the method of preparing the stomach-friendly and anti-inflammatory synergistic complex capsule is characterized by, The method comprises the following steps: After mixing the lactic acid bacteria dry powder, the egg yolk globulin and the dandelion extract for 10 minutes, the fructooligosaccharides, the malt dextrin and the micro-powder silica gel are added, and then mixed in a mixing machine for 15 minutes; the mixed powder is filled into a capsule shell using a capsule filling machine, bottled, labeled and packaged.

9. The method of claim 8, wherein the method is characterized by the steps of: The capsule has a loading capacity of 380-420 mg.

10. The stomach-friendly and anti-inflammatory synergistic compound capsule according to claim 1, wherein The complex capsule can improve the survival rate of lactic acid bacteria in gastric acid, from 30% to 81%.