Small-molecular organic carbon water-soluble fertilizer for promoting lignification of eucalyptus and preparation method and application of small-molecular organic carbon water-soluble fertilizer

Abstract

The invention provides a micromolecular organic carbon water-soluble fertilizer for promoting lignification of eucalyptus, and a preparation method and application thereof, and belongs to the technical crossing field of microorganisms, forestry and organic water-soluble fertilizers. The micromolecular organic carbon water-soluble fertilizer for promoting eucalyptus lignification comprises the following components in parts by weight: 3-5 parts of a biological agent, 10-20 parts of food industry leftovers, 20-30 parts of an organic solid fertilizer, 5-15 parts of sodium silicate, 10-20 parts of monocalcium phosphate, 5-15 parts of potassium chloride, 5-15 parts of magnesium nitrate, 5-15 parts of sulfur coated urea, 0.1-0.5 part of a rooting agent, 0.5-1 part of a trace element composite nutrient solution, 5-50 parts of deionized water and 1-3 parts of a pH regulator. The biological agent is prepared by fermenting a new bacillus tequilensis strain, namely, bacillus tequilensis XD63Y, and the collection number of the bacillus tequilensis XD63Y is GDMCC No: 62521. The organic solid fertilizer is prepared by crushing plant straws and then performing composting or fermentation treatment. The micromolecular organic carbon water-soluble fertilizer provided by the invention can promote the growth and lignification process of the eucalyptus, shorten the growth cycle of the eucalyptus, increase the yield of the eucalyptus and enhance the disease and pest resistance of the eucalyptus.

Description

TECHNICAL FIELD

[0001] The application discloses a small-molecule organic carbon water-soluble fertilizer for promoting eucalyptus lignification, a preparation method and application thereof, in particular to a small-molecule organic carbon water-soluble fertilizer with high silicon, calcium, phosphorus and potassium prepared by Bacillus Tequilaensis metabolites, belonging to the technical cross field of microorganisms, forestry and organic water-soluble fertilizer. BACKGROUND

[0003] The prior art still has the following problems: 1. Long growth cycle: Although eucalyptus grows fast, the period to reach the standard of usable wood is still long, usually 6 to 7 years or even longer. Planters are afraid of typhoon impact and dare not to fertilize in the first three years of eucalyptus growth, and let it grow slowly naturally. After 3 to 4 years, the tree trunk gradually lignifies, and then fertilization begins, greatly prolonging the harvesting time of eucalyptus. How to shorten the growth cycle of eucalyptus and achieve rapid and efficient wood production is an important research topic in current forestry. 2. Slow lignification process: The lignification process of eucalyptus, i.e. the process of lignin deposition in cell wall to form hard wood, needs a long time. This process is crucial for the strength and quality of wood, and traditional fertilizers and management methods are difficult to significantly accelerate the lignification process. 3. Low nutrient absorption efficiency: Eucalyptus needs a large amount of nutrients during growth, and the lignification process needs a large amount of small-molecule organic carbon, combined with silicon, calcium, phosphorus and potassium and other mineral elements. However, the existing fertilizer formula often cannot meet the needs of eucalyptus for small-molecule organic carbon nutrients and various mineral elements in the process of rapid growth and lignification, resulting in low nutrient absorption efficiency and poor growth effect. 4. Large environmental impact: Traditional chemical fertilizers, while improving plant growth rate and yield, often cause certain negative effects on soil and environment, such as soil acidification, nutrient loss and water pollution, etc. This poses a challenge to the sustainable development of ecological environment. 5. Traditional compound fertilizer is unbalanced in nutrition and lacks organic matter wrapping. After being applied to soil, it is easily washed away by rainwater, thus shortening the fertilizer effect, lowering the absorption and utilization rate, and causing damage to the soil.

[0004] Therefore, it is urgent to develop a fertilizer rich in small-molecule organic carbon, combined with high silicon, calcium, phosphorus and potassium, and easy to be absorbed by eucalyptus, for promoting the growth of eucalyptus, shortening the growth cycle of eucalyptus and accelerating the lignification process of eucalyptus. SUMMARY

[0005] Based on the above background, the present application provides a small-molecule organic carbon water-soluble fertilizer for promoting eucalyptus lignification and a preparation method thereof, aiming to improve the nutrient utilization efficiency of eucalyptus, realize synchronous lignification of eucalyptus growth, shorten the growth cycle of eucalyptus, improve the yield of eucalyptus, and reduce the negative impact on the environment, thereby providing a new way for efficient production and sustainable development of eucalyptus.

[0006] Through the synergistic effect of the abundant small-molecule organic carbon materials produced by Bacillus tequilensis metabolites and plant straw fermentation and the high-silicon, high-calcium, high-phosphorus and high-potassium components, the healthy growth of eucalyptus root system can be promoted, the stress resistance and disease and pest resistance of plants can be enhanced, and the lignification process of eucalyptus can be comprehensively improved. This innovative method not only provides a new technical solution for eucalyptus planting, but also provides a reference and reference for the efficient planting and management of other economic tree species.

[0007] To achieve the purpose of the application, the technical scheme adopted by the present application is as follows: according to a small-molecule organic carbon water-soluble fertilizer for promoting eucalyptus lignification, the composition of the small-molecule organic carbon water-soluble fertilizer for promoting eucalyptus lignification is as follows: 3-5 parts of biological agent, 10-20 parts of food industry waste, 20-30 parts of organic solid fertilizer, 5-15 parts of sodium silicate, 10-20 parts of calcium dihydrogen phosphate, 5-15 parts of potassium chloride, 5-15 parts of magnesium nitrate, 5-15 parts of sulfur-coated urea, 0.1-0.5 parts of rooting agent, 0.5-1 parts of trace element composite nutrient solution, 5-50 parts of deionized water, and 1-3 parts of pH adjuster; the biological agent is obtained by fermentation of a new strain of Bacillus tequilensis XD63Y, which has a preservation number of GDMCC No: 62521; the preparation steps of the organic solid fertilizer are as follows: collecting plant straw and crushing it to a particle size of less than 5 mm, and then composting or fermenting the crushed waste, maintaining the moisture content of the compost pile at 50-60% during the composting process, turning the compost every 3 days, controlling the fermentation temperature at 50-60℃, and collecting the compost product after fermentation, drying and crushing it to a particle size of less than 1 mm to obtain an organic solid fertilizer rich in organic nutrients.

[0008] According to a small-molecule organic carbon water-soluble fertilizer for promoting eucalyptus lignification, the composition of the food industry waste is as follows: 5-20 parts of distiller's grain concentrate waste liquid, 5-20 parts of monosodium glutamate concentrate waste liquid, and 5-20 parts of soy sauce concentrate waste liquid; the organic matter content of the distiller's grain concentrate waste liquid is 45-60 wt%, the organic matter content of the monosodium glutamate concentrate waste liquid is 40-60 wt%, and the organic matter content of the soy sauce concentrate waste liquid is 40-60 wt%.

[0009] According to a kind of small molecule organic carbon water-soluble fertilizer promoting eucalyptus lignification, the trace element composite nutrient solution is chelated iron (EDTA~Fe): 0.01~0.1 parts, zinc nitrate (Zn (NO3) 2): 0.01~0.05 parts, copper nitrate (Cu (NO3) 2): 0.005~0.02 parts, manganese nitrate (Mn (NO3) 2): 0.01~0.05 parts, sodium molybdate (Na2MoO4): 0.001~0.01 parts, boric acid (H3BO3): 0.01~0.05 parts.

[0010] According to a kind of small molecule organic carbon water-soluble fertilizer promoting eucalyptus lignification, the pH regulator is potassium hydrogen phosphate and potassium dihydrogen phosphate.

[0011] According to a kind of small molecule organic carbon water-soluble fertilizer promoting eucalyptus lignification, the plant straw is one or more of wheat straw, cotton straw and corn straw.

[0012] According to a kind of small molecule organic carbon water-soluble fertilizer promoting eucalyptus lignification, the plant growth regulator is indole acetic acid.

[0013] According to a kind of small molecule organic carbon water-soluble fertilizer promoting eucalyptus lignification, the water-soluble fertilizer is divided into two forms of paste and liquid, the preparation method of paste fertilizer, including the following steps: S1 Bacillus tectum XD63Y is inoculated into culture medium, and a constant temperature water bath shaker is used to shake the bottle at a slow speed of 40~50 DEG C and 10~30 revolutions per minute for 24~96 hours; wherein, the culture medium comprises: 500ml of distilled water, 5~40% of glucose, 0.3~3% of yeast extract, 0.5~5% of N, 0.3~3% of P2O5, 0.1~2% of K2O, 0.05~1% of trace elements, and pH is 5.5~8.5; S2 the plant straw after crushing is composted or fermented, the stack humidity is kept at 50~60%, the stack is turned over once every 3 days, the fermentation is carried out for 30~45 days, the temperature is controlled at 50~60 DEG C, after the fermentation is finished, drying and crushing to particle size less than 1 millimeter, organic solid fertilizer is obtained; S3 according to the formula amount, in multifunctional reaction kettle, bacillus tectum metabolic product, food industry waste, organic solid fertilizer, trace element composite nutrient solution and deionized water are added in sequence, the temperature is adjusted to 40~50 DEG C, the stirring speed is 100~200 revolutions per minute, stirring is carried out for 12~24 hours, during which pH regulator is used to keep the pH of mixed solution at 6.0~7.5; S4 sodium silicate, calcium dihydrogen phosphate and potassium chloride are added in the reaction kettle, the temperature is adjusted to 30~40 DEG C, the stirring speed is 100~200 revolutions per minute, continue stirring for 2~4 hours, and cool to room temperature after standing, to obtain organic small molecule water-soluble fertilizer; S5 Put the prepared paste into a degradable biological film package, with a mass of 200-1000g per bag, and place the packaged product in a dry and cool place to avoid direct sunlight and high-temperature environment.

[0014] The preparation method of the liquid fertilizer comprises the following steps: S1 According to the formula, add Bacillus tectivirus metabolic products, food industry waste, trace element compound nutrient solution and deionized water into a multifunctional reaction kettle in sequence, adjust the temperature to 40-50 DEG C, the stirring speed is 100-200 r / min, and stirring is performed for 12-24 hours; during the stirring, a pH regulator is used to keep the pH of the mixed solution at 6.0-7.5; S2 The prepared liquid fertilizer is filtered through a filter screen to remove solid impurities, and then is sealed and stored in a bottle; the prepared liquid fertilizer is placed in a dry and cool place to avoid direct sunlight and high-temperature environment.

[0015] According to a small-molecule organic carbon water-soluble fertilizer for promoting eucalyptus lignification, the small-molecule organic carbon refers to an organic matter composed of 3 or fewer carbons, which can be rapidly decomposed by microorganisms into water and carbon dioxide, thereby providing energy and carbon elements for an ecological system.

[0016] According to a small-molecule organic carbon water-soluble fertilizer for promoting eucalyptus lignification, the fertilizer has two forms: a liquid form and a paste form, and can be applied by pouring or spraying and irrigating; during the growth period of the eucalyptus, the fertilizer is applied every 2-3 months, the application amount is adjusted according to the growth of the eucalyptus, 2-3 L of water is irrigated after the application of the fertilizer, the growth status of the eucalyptus, including the tree height, stem diameter, leaf color and pest and disease conditions, is monitored regularly after the application of the fertilizer, the effect of the fertilizer is evaluated, and the application scheme is adjusted according to the monitoring result.

[0017] The growth cycle is significantly shortened: the present application produces abundant small-molecule organic carbon by fermenting plant straw with Bacillus tectivirus, and combines the small-molecule organic carbon water-soluble fertilizer with high silicon, high calcium, high phosphorus and high potassium, thereby significantly accelerating the growth speed and lignification process of the eucalyptus. The use of the rooting agent promotes the rooting rate of the eucalyptus in the seedling stage and enhances the ability of the eucalyptus seedling to resist typhoons. Compared with the traditional fertilizer, the small-molecule organic water-soluble fertilizer produced by the present application can be applied in the first year of eucalyptus planting, promotes the lignification process of the eucalyptus, and can shorten the growth cycle of the eucalyptus to 4-6 years, thereby greatly improving the wood production efficiency.

[0018] The disease and pest resistance is enhanced: the various organic acids and enzymes in the metabolic products of Bacillus tectivirus can significantly improve the disease and pest resistance of the eucalyptus, reduce the influence of diseases and pests on the growth of the eucalyptus, and further enhance the physical defense ability of the eucalyptus by adding high-silicon ingredients, thereby improving the adaptability of the eucalyptus to environmental stress and reducing the use of pesticides, and improving the healthy growth level of the eucalyptus.

[0019] High-value utilization of plant straw: Using agricultural by-products, such as corn straw and wheat straw, as raw materials for high-value utilization can not only reduce the environmental burden caused by straw burning or the plant diseases caused by straw, but also obtain raw materials locally and reduce transportation costs.

[0020] Reducing Environmental Burden: The long-term use of traditional chemical fertilizers often has negative impacts on soil and the environment, such as soil acidification, nutrient loss, and water pollution. This invention, by using Bacillus tekirae metabolites and a highly efficient mineral element formula, not only improves fertilizer utilization but also reduces the use of chemical components, thereby reducing pollution to soil and the environment and contributing to the sustainable development of the ecological environment.

[0021] Improving nutrient absorption efficiency: The metabolites of *Bacillus tekirae* significantly enhance the vitality of eucalyptus roots, increasing root surface area and absorption capacity. Simultaneously, the scientifically formulated high-silicon, high-calcium, high-phosphorus, and high-potassium content comprehensively meets the nutrient requirements of eucalyptus at different growth stages. Compared to traditional fertilizers, this long-acting topdressing improves the efficiency of eucalyptus nutrient absorption and utilization, reducing fertilizer waste and nutrient loss. Attached Figure Description

[0022] Figure 1 This is the DNA sequencing diagram of the present invention; Figure 2 , 3 4 are optical microscope images of the small molecule organic fertilizer of the present invention. Detailed Implementation

[0023] Example 1

[0024] 1. Domestication and screening of strains Six strains of Bacillus tekirae, numbered XD51Y to XD56Y, were purchased from a bacterial culture center and Taobao. Fifteen strains with cell and colony morphology similar to Bacillus tekirae, numbered XD57Y to XD71Y, were then screened from soil and stinking ditches around Zhongxin Village, Taoyuan Town, Heshan City, Jiangmen, Guangdong Province. These strains were subjected to 20 generations of anaerobic mutagenesis culture, and finally, the strain with the strongest oxygen production capacity in an anaerobic environment was selected.

[0025] 2. Preparation of the above-mentioned anaerobic environment and special culture medium for mutagenesis culture A 1000 ml glass beaker is filled with 900 ml distilled water and 2-20 g of acclimation medium is added. 5 ml of 50-fold diluted sodium sulfite solution is added every 12 hours to remove dissolved oxygen in the water body. The acclimation medium has the following formulation: 5-40% glucose, 0.3-3% yeast extract, 0.5-6% potassium nitrate, 0.3-5% diammonium phosphate, 0.2-2% sodium chloride, 0.02-0.1% zinc nitrate, 0.02-0.1% manganese nitrate, 0.01-0.1% boric acid, and 0.02-0.15% ferric nitrate. Distilled water is added to make up the volume to 100 ml, and the pH is adjusted to 6.5-7.5.

[0026] 3. Cultivation method The 66 beakers containing the culture solution are inoculated with the 21 strains of bacteria, with 3 bottles for each strain. The remaining 3 bottles are used as blank controls. After 48-96 hours of cultivation in a 30-45°C incubator, the dissolved oxygen in the culture solution is measured.

[0027] Table 1: Average dissolved oxygen of the first 7 strains in each test group during the first generation acclimation process

[0028] Table 2: Average dissolved oxygen of the first 7 strains in each test group during the 10th generation acclimation process

[0029] Table 3: Average dissolved oxygen of the first 7 strains in each test group during the 20th generation acclimation process

[0030] 4. Screening method The goal of the screening is to select the strain with the highest dissolved oxygen in the culture solution. First, the top 7 strains with the highest dissolved oxygen in all the culture bottles are selected, and their bacterial strains are isolated and purified. After cultivation, one bacterial colony with the closest morphology to Bacillus tequilensis is selected from each culture dish for the next round of acclimation cultivation. This process is repeated for a total of 20 generations of acclimation and mutation cultivation and screening, and the strain with the strongest ability to increase dissolved oxygen in the water body is finally selected.

[0031] Table 4: Dissolved oxygen in the culture solution of XD63Y during the first, 10th, and 20th generations of acclimation process

[0032] 5. Screening results The final selected strain is named XD63Y. After purification and purification cultivation, it was sent to the Guangdong Academy of Microbiology for DNA sequencing on June 7, 2022. The sequencing results are as follows: Figure 1As shown, the results prove that the strain is a strain of Bacillus tequilensis, and has been preserved by Guangdong Microbial Culture Collection Center, and received the preservation accession number GDMCC No: 62521, and the preservation unit address is No. 59, Building 5, Institute of Microbiology, Guangdong Academy of Sciences, 100, Martyrs' Courtyard, Guangzhou.

[0033] Example Two

[0034] 1. Organic waste collection and crushing: Collect corn straw and crush it to a particle size of less than 5 mm.

[0035] 2. Composting: The crushed straw is composted, and the moisture content of the compost is maintained at 50% during the composting process, and the temperature is controlled at 50°C. Turn the pile every 3 days, and the fermentation time is 35 days. After fermentation, collect the compost product, dry and crush it to a particle size of less than 1 mm to obtain an organic solid fertilizer rich in organic nutrients.

[0036] Example Three

[0037] 1. Organic waste collection and crushing: Collect corn straw and crush it to a particle size of less than 5 mm.

[0038] 2. Composting: The crushed straw is composted, and the moisture content of the compost is maintained at 55% during the composting process, and the temperature is controlled at 55°C. Turn the pile every 3 days, and the fermentation time is 35 days. After fermentation, collect the compost product, dry and crush it to a particle size of less than 1 mm to obtain an organic solid fertilizer rich in organic nutrients.

[0039] Example Four

[0040] 1. Organic waste collection and crushing: Collect corn straw and crush it to a particle size of less than 5 mm.

[0041] 2. Composting: The crushed straw is composted, and the moisture content of the compost is maintained at 60% during the composting process, and the temperature is controlled at 60°C. Turn the pile every 3 days, and the fermentation time is 35 days. After fermentation, collect the compost product, dry and crush it to a particle size of less than 1 mm to obtain an organic solid fertilizer rich in organic nutrients.

[0042] Example Five

[0043] 1. Mixing of ingredients: Prepare the following ingredients by weight: Bacillus tequilensis metabolites: 3 parts; food industry waste: 10 parts; organic solid fertilizer: 20 parts; sodium silicate: 5 parts; calcium dihydrogen phosphate: 10 parts; potassium chloride: 5 parts; magnesium nitrate: 5 parts; sulfur-coated urea: 5 parts; rooting agent: 0.1 parts; trace element compound nutrient solution: 0.5 parts; deionized water: 5 parts; pH adjuster: potassium dihydrogen phosphate, potassium hydrogen phosphate: 1 part; 2. Fertilizer preparation: Bacillus tectum XD63Y is inoculated into the culture medium, and a constant temperature water bath shaker is used for slow shaking at 40°C and 10 rpm for 24 hours. The culture medium comprises 500 ml of distilled water, 5% of glucose, 0.3% of yeast extract, 0.5% of N, 0.3% of P2O5, 0.1% of K2O, 0.05% of trace elements, and the pH is 6; The crushed plant straw is subjected to composting or fermentation treatment, the pile humidity is kept at 50%, the pile is turned over every 3 days, the fermentation is performed for 30 days, the temperature is controlled at 50°C, after the fermentation is completed, drying and crushing are performed to obtain the organic solid fertilizer with a particle size of less than 1 mm; The formula amount is added to a multifunctional reaction kettle, and the Bacillus tectum metabolic product, food industry waste, organic solid fertilizer, trace element compound nutrient solution and deionized water are sequentially added, the temperature is adjusted to 40°C, the stirring speed is 100 rpm, and stirring is performed for 12 hours, during which the pH of the mixed solution is kept at 6 by using a pH adjuster; In the reaction kettle, sodium silicate, calcium dihydrogen phosphate and potassium chloride are added, the temperature is adjusted to 30°C, the stirring speed is 100 rpm, and stirring is continued for 2 hours, and the reaction kettle is cooled to room temperature to obtain the organic small molecule water-soluble fertilizer Fertilizer storage: The prepared paste fertilizer is sealed in a degradable biological film package and stored in a dry and cool place to avoid direct sunlight and high temperature environment. The liquid fertilizer needs to be sealed and stored to prevent evaporation and pollution.

[0044] The prepared paste fertilizer is placed under an optical microscope for observation, and the observation results are shown in Figure 2 .

[0045] Example Six

[0046] 1. Component mixing: The following components are prepared according to the weight fractions: Bacillus tectum metabolic product: 4 parts; food industry waste: 15 parts; organic solid fertilizer: 25 parts; sodium silicate: 10 parts; calcium dihydrogen phosphate: 15 parts; potassium chloride: 10 parts; magnesium nitrate: 10 parts; sulfur-coated urea: 10 parts; rooting agent: 0.3 parts; trace element compound nutrient solution: 1 part; deionized water: 30 parts; pH adjuster: potassium dihydrogen phosphate and potassium hydrogen phosphate: 2 parts; 2. Fertilizer preparation: Bacillus tectum XD63Y is inoculated into the culture medium, and a constant temperature water bath shaker is used for slow shaking at 45°C and 20 rpm for 48 hours. The culture medium comprises 500 ml of distilled water, 20% of glucose, 1% of yeast extract, 3% of N, 2% of P2O5, 1% of K2O, 0.5% of trace elements, and the pH is 7; The crushed plant straw is subjected to composting or fermentation treatment, the pile humidity is kept at 55%, the pile is turned over once every 3 days, fermentation is carried out for 45 days, the temperature is controlled at 55 DEG C, after fermentation, drying and crushing to a particle size of less than 1mm, organic solid fertilizer is obtained; According to the formula, in the multifunctional reaction kettle, Bacillus Tekila metabolic products, food industry waste, organic solid fertilizer, trace element compound nutrient solution and deionized water are added in turn, the temperature is adjusted to 45 DEG C, the stirring speed is 150 r / min, stirring for 24 hours, during which the pH of the mixed solution is kept at 7 by using pH regulator; In the reaction kettle, sodium silicate, calcium dihydrogen phosphate, potassium chloride are added, the temperature is adjusted to 35 DEG C, the stirring speed is 150 r / min, continue stirring for 3 hours, cool to room temperature, get organic small molecule water-soluble fertilizer Fertilizer storage: the prepared paste fertilizer is sealed in degradable biological film package and stored in dry and cool place to avoid direct sunlight and high temperature environment. Liquid fertilizer needs to be sealed and stored to prevent evaporation and pollution.

[0047] The prepared paste fertilizer is placed under optical microscope for observation, and the observation results are shown in Figure 3 .

[0048] Example seven

[0049] 1, component mixing: prepare each component according to the following weight fraction: bacillus tekila metabolic products: 5 parts; food industry waste: 20 parts; organic solid fertilizer: 30 parts; sodium silicate: 15 parts; calcium dihydrogen phosphate: 20 parts; potassium chloride: 15 parts; magnesium nitrate: 15 parts; sulfur coated urea: 15 parts; rooting agent: 0.5 parts; trace element compound nutrient solution: 1 part; deionized water: 50 parts; pH regulator: potassium dihydrogen phosphate, potassium hydrogen phosphate: 3 parts; 2, fertilizer preparation: bacillus tekila XD63Y is inoculated into culture medium, constant temperature water bath shaker, 50 DEG C, 30 r / min slow speed shaking flask, shaking flask 96 hours; wherein, the culture medium contains: distilled water 500ml, glucose 40%, yeast extract 3%, N 5%, P2O5 3%, K2O2%, trace elements 1%, pH 7.5; The crushed plant straw is subjected to composting or fermentation treatment, the pile humidity is kept at 60%, the pile is turned over once every 3 days, fermentation is carried out for 45 days, the temperature is controlled at 60 DEG C, after fermentation, drying and crushing to a particle size of less than 1mm, organic solid fertilizer is obtained; According to the formula, in the multifunctional reaction kettle, bacillus tekila metabolic products, food industry waste, organic solid fertilizer, trace element compound nutrient solution and deionized water are added in turn, the temperature is adjusted to 50 DEG C, the stirring speed is 200 r / min, stirring for 96 hours, during which the pH of the mixed solution is kept at 7.5 by using pH regulator; In the reaction kettle, sodium silicate, calcium dihydrogen phosphate, potassium chloride were added, the temperature was adjusted to 40℃, the stirring speed was 200 revolutions per minute, and the stirring was continued for 4 hours, and then the mixture was cooled to room temperature to obtain an organic small molecule water-soluble fertilizer 3. Fertilizer storage: the prepared paste fertilizer was sealed in a degradable biological film package and stored in a dry and cool place to avoid direct sunlight and high temperature environment. Liquid fertilizer should be sealed and stored to prevent evaporation and pollution.

[0050] 4. The prepared paste fertilizer was placed under an optical microscope for observation, and the observation results were as shown in Figure 4 .

[0051] Example Eight

[0052] 1. Ingredient mixing: prepare each ingredient according to the following weight fraction: food industry waste: 10 parts; sodium silicate: 5 parts; calcium dihydrogen phosphate: 10 parts; potassium chloride: 5 parts; magnesium nitrate: 5 parts; sulfur-coated urea: 5 parts; rooting agent: 0.1 part; trace element composite nutrient solution: 0.5 part; deionized water: 5 parts; pH adjuster: potassium dihydrogen phosphate, potassium hydrogen phosphate: 1 part; 2. According to the formula, food industry waste, trace element composite nutrient solution and deionized water were added in a multifunctional reaction kettle, the temperature was adjusted to 40℃, the stirring speed was 100 revolutions per minute, and the stirring was continued for 12 hours, and the pH of the mixed solution was maintained at 6 during the period using the pH adjuster; 3. In the reaction kettle, sodium silicate, calcium dihydrogen phosphate, potassium chloride were added, the temperature was adjusted to 30℃, the stirring speed was 100 revolutions per minute, and the stirring was continued for 2 hours, and then the mixture was cooled to room temperature to obtain an organic small molecule water-soluble fertilizer 4. Fertilizer storage: the prepared liquid fertilizer was sealed in a 1L plastic bottle and stored in a dry and cool place to avoid direct sunlight and high temperature environment. Liquid fertilizer should be sealed and stored to prevent evaporation and pollution.

[0053] Example Nine

[0054] 1. Ingredient mixing: prepare each ingredient according to the following weight fraction: food industry waste: 15 parts; sodium silicate: 10 parts; calcium dihydrogen phosphate: 15 parts; potassium chloride: 10 parts; magnesium nitrate: 10 parts; sulfur-coated urea: 10 parts; rooting agent: 0.3 parts; trace element composite nutrient solution: 1 part; deionized water: 30 parts; pH adjuster: potassium dihydrogen phosphate, potassium hydrogen phosphate: 2 parts; 2. According to the formula, food industry waste, trace element composite nutrient solution and deionized water were added in a multifunctional reaction kettle, the temperature was adjusted to 40℃, the stirring speed was 100 revolutions per minute, and the stirring was continued for 12 hours, and the pH of the mixed solution was maintained at 6 during the period using the pH adjuster; 3. Add sodium silicate, calcium dihydrogen phosphate, and potassium chloride into the reactor, adjust the temperature to 30°C, and stir at a speed of 100 rpm for 2 hours. Let it cool to room temperature to obtain the organic small-molecule water-soluble fertilizer. 4. Fertilizer storage: The prepared liquid fertilizer is sealed in a 1L plastic bottle and stored in a dry and cool place to avoid direct sunlight and high temperature environment. The liquid fertilizer needs to be stored in a sealed manner to prevent evaporation and contamination.

[0055] Example Ten

[0056] 1. Ingredient mixing: Prepare the following ingredients by weight: Bacillus tectivirus metabolite: 5 parts; food industry waste: 20 parts; organic solid fertilizer: 30 parts; sodium silicate: 15 parts; calcium dihydrogen phosphate: 20 parts; potassium chloride: 15 parts; magnesium nitrate: 15 parts; sulfur-coated urea: 15 parts; rooting agent: 0.5 parts; trace element composite nutrient solution: 1 part; deionized water: 50 parts; pH adjuster: potassium dihydrogen phosphate and potassium hydrogen phosphate: 3 parts. 2. According to the formula, add food industry waste, trace element composite nutrient solution, and deionized water in a multifunctional reactor, adjust the temperature to 40°C, and stir at a speed of 100 rpm for 12 hours. During this period, use the pH adjuster to maintain the pH of the mixed solution at 6. 3. Add sodium silicate, calcium dihydrogen phosphate, and potassium chloride into the reactor, adjust the temperature to 30°C, and stir at a speed of 100 rpm for 2 hours. Let it cool to room temperature to obtain the organic small-molecule water-soluble fertilizer. 4. Fertilizer storage: The prepared liquid fertilizer is sealed in a 1L plastic bottle and stored in a dry and cool place to avoid direct sunlight and high temperature environment. The liquid fertilizer needs to be stored in a sealed manner to prevent evaporation and contamination.

[0057] Example Eleven

[0058] The small-molecule organic carbon water-soluble fertilizer prepared in Examples Five, Six, Seven is tested for elements and pH to evaluate whether the prepared organic fertilizer meets the requirements of fertilizer production. The results are shown in the following table:

[0059] According to the national standard, the total amount of nitrogen, phosphorus, silicon, and potassium in organic water-soluble fertilizer needs to exceed 200 g / kg, and the pH value needs to be in the range of 5.0~7.0. Therefore, the water-soluble organic fertilizer prepared in Examples Five, Six, Seven, Eight, Nine, Ten meets the requirements.

[0060] Example Twelve

[0061] 1. Fertilization time and frequency: In the eucalyptus growing season, fertilization is carried out every 2 months in spring and summer.

[0062] 2. Fertilization method: Based on the planting density and root distribution of eucalyptus trees, determine the amount of paste fertilizer with a package weight of 200g to be placed at the root of each eucalyptus tree. After fertilization, irrigate 2L of water to ensure that the fertilizer fully penetrates the root zone.

[0063] 3. Effect monitoring: Regularly monitor the growth conditions of eucalyptus trees, including tree height, trunk diameter, leaf color, and pest and disease conditions. Compare with the non-fertilized area to evaluate the effect of the fertilizer, and adjust the fertilization scheme according to the monitoring results.

[0064] Example XIII

[0065] 1. Fertilization time and frequency: In the spring and summer, fertilize every 3 months.

[0066] 2. Fertilization method: Based on the planting density and root distribution of eucalyptus trees, determine the amount of paste fertilizer with a package weight of 500g to be placed at the root of each eucalyptus tree. After fertilization, irrigate 3L of water to ensure that the fertilizer fully penetrates the root zone.

[0067] 3. Effect monitoring: Regularly monitor the growth conditions of eucalyptus trees, including tree height, trunk diameter, leaf color, and pest and disease conditions. Compare with the non-fertilized area to evaluate the effect of the fertilizer, and adjust the fertilization scheme according to the monitoring results.

[0068] Example XIV

[0069] 1. Fertilization time and frequency: In the spring and summer, fertilize every 3 months.

[0070] 2. Fertilization method: Based on the planting density and root distribution of eucalyptus trees, determine the amount of paste fertilizer with a package weight of 1000g to be placed at the root of each eucalyptus tree. After fertilization, irrigate 3L of water to ensure that the fertilizer fully penetrates the root zone.

[0071] 3. Effect monitoring: Regularly monitor the growth conditions of eucalyptus trees, including tree height, trunk diameter, leaf color, and pest and disease conditions. Compare with the non-fertilized area to evaluate the effect of the fertilizer, and adjust the fertilization scheme according to the monitoring results.

[0072] Example XV

[0073] 1. Fertilization time and frequency: In the spring and summer, fertilize every 3 months.

[0074] 2. Fertilization method: Dilute the liquid fertilizer with water at a ratio of 1:100 before use. The diluted liquid fertilizer is applied by irrigation, evenly irrigating around the roots of the eucalyptus trees. After fertilization, irrigate 1L of water to ensure that the fertilizer fully penetrates the root zone.

[0075] 3. Effect monitoring: Regularly monitor the growth conditions of eucalyptus, including tree height, trunk diameter, leaf color, and pest and disease conditions. By comparing with the non-fertilized area, evaluate the effect of fertilizer, and adjust the fertilization scheme according to the monitoring results.

[0076] Example Sixteen

[0077] In order to verify the effect of the small-molecule organic carbon and water soluble fertilizer of the present application in enhancing the disease and pest resistance of eucalyptus, the following experiment was conducted: 1. Experimental materials: Eucalyptus seedlings: Select eucalyptus seedlings with consistent growth conditions, a total of 100, divided into treatment group and control group. Small-molecule organic carbon and water soluble fertilizer: prepared according to the formula of claim 1. Control group: use commercially available ordinary compound fertilizer.

[0078] 2. Experimental method: (1) Fertilization treatment: Treatment group: 200 grams of small-molecule organic carbon and water soluble fertilizer described in examples five, six and seven were applied to each eucalyptus seedling, and applied once every two months.

[0079] Control group: 200 grams of commercially available ordinary compound fertilizer was applied to each eucalyptus seedling, and applied once every two months.

[0080] (2) Environmental conditions: Experimental site: the forest of Xinxin Biological Products Co., Ltd. in Heshan City, Jiangmen, Guangdong Province.

[0081] Irrigation management: consistent irrigation frequency and water volume.

[0082] 3. Experimental observation index: (1) Disease and pest incidence: record the disease and pest occurrence of each eucalyptus seedling during the entire growth period.

[0083] (2) Growth index: record the height, trunk diameter and leaf number of each eucalyptus seedling.

[0084] 4. Experimental results after two years of cultivation period:

[0085] 5. Data analysis (1) Disease and pest incidence: the disease and pest incidence of the treatment group was 10%, which was significantly lower than that of the control group of 35%, indicating that the small-molecule organic carbon and water soluble fertilizer of the present application significantly improved the disease and pest resistance of eucalyptus.

[0086] (2) Growth index: the average height, trunk diameter and leaf number of the treatment group were significantly higher than those of the control group, indicating that the fertilizer of the present application not only improved the disease and pest resistance, but also promoted the healthy growth of eucalyptus.

[0087] 6、Conclusion: The experimental results show that the small molecule organic carbon water-soluble fertilizer can significantly improve the disease and pest resistance of eucalyptus through the synergistic effect of organic acids and enzymes in Bacillus tectum metabolic products and high-silicon components, and reduce the influence of diseases and pests on eucalyptus. At the same time, the common action of various nutrients and biological agents in the fertilizer promotes the healthy growth and lignification of eucalyptus, and improves the wood quality and yield.

[0088] Example seventeen

[0089] In order to detect the absorption efficiency of eucalyptus to silicon, phosphorus, calcium, potassium and trace elements after using the small molecule organic carbon water-soluble fertilizer of the application, the following experiment was conducted: 1. Select 60 eucalyptus seedlings with consistent growth conditions and divide them into experimental group 1, experimental group 2, experimental group 3 and control group.

[0090] 2. The experimental group 1, 2 and 3 respectively use the organic small molecule water-soluble fertilizer described in examples 5, 6 and 7, 200g, and fertilize every 2 months; the control group uses commercially available ordinary fertilizer, 200g, and fertilizes every 2 months.

[0091] 3. Collect eucalyptus leaf, branch and root samples regularly every month, and use plant tissue analyzer to measure the content of nitrogen, phosphorus, potassium, silicon and trace elements, and take their average value.

[0092] 4. The experimental results are as follows:

[0093] According to the following formula, the absorption efficiency of eucalyptus is calculated as follows: Formula:

[0094]

[0095] Conclusion: The nutrient absorption rate of the treated eucalyptus is significantly higher than that of the control group, which proves that the use of small molecule organic carbon water-soluble fertilizer can significantly improve the absorption rate of eucalyptus to nitrogen, phosphorus, potassium, silicon and trace elements. This shows that the fertilizer has a significant effect on improving the nutrient utilization efficiency of eucalyptus.

[0096] Technical personnel should know: although the application has been described according to the above specific embodiments, the inventive idea of the application is not limited to this application, and any modification using the inventive idea will be included in the scope of protection of this patent.

[0097] Example eighteen

[0098] The prepared small molecule organic carbon water-soluble fertilizer was tested for lodging resistance to evaluate whether the prepared organic fertilizer is suitable for use in the seedling stage: Fertilization treatment: select 60 eucalyptus seedlings with the same growth conditions, and divide them into an experimental group and a control group, 30 in each. The experimental group applies 200 g of the small-molecule organic water-soluble fertilizer of the present application to each eucalyptus seedling, once every two months. The control group applies 200 g of ordinary waste material on the market to each eucalyptus seedling, once every two months.

[0099] Wind simulation: use a wind simulation device (blower) to simulate wind at a wind speed of 7 levels, test the seedling's resistance to lodging under the action of wind, and implement it once every two months, with an action time of 5 minutes each time Record the survival rate of eucalyptus seedlings after one year, and the experimental data are recorded in the following table:

[0100] Conclusion: The small-molecule organic water-soluble fertilizer of the present application can greatly improve the resistance to lodging of eucalyptus seedlings and enhance the survival ability of eucalyptus seedlings in typhoon environment.

[0101] Example Nineteen

[0102] Water-soluble small-molecule organic carbon content determination 1. Method principle The principle is to extract humus in the sample with an extractant (0.1 mol / L Na2P4O7+0.1 mol / L NaOH, pH 13), and then acidify to pH 2 with H2SO4 solution, and then filter the small-molecule organic carbon solution obtained by 0.45 um microporous filter membrane.

[0103] The water-soluble small-molecule organic carbon in the sample is oxidized with a quantitative potassium dichromate-sulfuric acid solution, and the remaining potassium dichromate is titrated with a ferrous sulfate standard titration solution. Based on the reagent blank, the water-soluble small-molecule organic carbon content is obtained according to the volume of oxidizing agent consumed before and after oxidation, with a carbon content of 0.4.

[0104] 2. Experimental instruments Laboratory commonly used instruments and equipment 3. Reagents and preparation Weigh 1.490 g of phenanthroline (analytical pure) and dissolve it in 100 mL of an aqueous solution containing 0.700 g of ferrous sulfate (analytical pure) to constant volume, and store it in a brown bottle.

[0105] Dissolve 15 g of crystalline sodium pyrophosphate (Na2P4O7·10H2O) and 7 g of sodium hydroxide in 1000 mL of water to constant volume, and store it in a brown bottle.

[0106] Take the potassium dichromate (reference reagent) 4.9031 g, which is baked at 130℃ for 3-4 hours, first dissolved with a small amount of water, then transferred into a 1 L volumetric flask, diluted to the mark with water, shaken and ready for use, and stored in a brown bottle.

[0107] Take 39.23 g of potassium dichromate (analytical pure), first dissolved with a small amount of water, then transferred into a 1 L volumetric flask, diluted to the mark, shaken and ready for use, and stored in a brown bottle.

[0108] Take 108.7 mL of sulfuric acid, dissolve in 700 mL of water solution, then transfer into a 1 L volumetric flask, dilute to the mark with water, shake and use.

[0109] Take (FeSO4·7H2O) (analytical pure) 55.6 g, dissolve in 900 mL of water, add 20 mL of sulfuric acid to dissolve, dilute to 1 L, shake and use (filter if necessary). The accurate concentration of this solution is calibrated with 0.1 mol / L potassium dichromate standard solution, and it is calibrated now.

[0110] c (FeSO4) = 0.2 mol / L standard solution calibration: take 20.00 mL of potassium dichromate standard solution into a 150 mL flask, add 3 mL-5 mL of sulfuric acid and 2 drops-3 drops of phenanthroline indicator, titrate with ferrous sulfate standard solution. According to the consumption amount when titrating with ferrous sulfate standard solution, calculate its accurate concentration c according to the following formula (A.1).

[0111]

[0112] In the formula: C1 is the concentration of potassium dichromate standard solution; V1 is the volume of potassium dichromate standard solution taken; V2 is the volume of ferrous sulfate standard solution consumed during titration; 4. Measurement steps Take 0.5 g-1.5 g of sample (accurate to 0.0002 g) into a 250 mL flask, add 100 mL of humus extractant (0.1 mol / L Na2P4O7+0.1 mol / L NaOH, pH 13), heat in a boiling water bath for 30 minutes, shake 3-4 times during the period, cool to room temperature, transfer into a centrifuge cup and centrifuge at 3000 r / min for 30 minutes, then pour the solution into a 250 mL volumetric flask, wash the insoluble matter, collect the washing liquid in a 250 mL volumetric flask by centrifugation, and dilute to the mark. A liquid.

[0113] Take 100 mL of solution A in a beaker, add 20 mL of 2 mol / L sulfuric acid solution, and centrifuge the suspension. After centrifugation, the upper clear liquid is filtered through a 0.45 um microporous filter to obtain a small molecular water-soluble organic carbon solution.

[0114] Take 10 mL of the small molecular water-soluble organic carbon solution in a 250 mL flask, accurately add 5 mL of potassium dichromate c (1 / 6 K2Cr2O7) = 0.8 mol / L solution and 15 mL of sulfuric acid, heat in a boiling water bath for 30 minutes, shake several times in the middle, and take out and cool. Dilute with water to about 100 mL, add 4 drops of phenanthroline indicator, and titrate to the end point (the solution changes from blue-green to brick red) with 0.2 mol / L ferrous sulfate standard solution. At the same time, a blank experiment is performed. If the volume consumed by the titration sample is less than 1 / 3 of the volume consumed by the titration blank, the sample weight should be reduced and re-measured.

[0115] 5. Expression of the analysis results The content of water-soluble small molecular water-soluble organic carbon in the sample is represented by y, which is calculated according to the following formula

[0116] In the formula: c (Fe 2+ ) - concentration of ferrous sulfate standard solution; V0 - volume of ferrous sulfate standard solution consumed in the blank determination; V1 - volume of ferrous sulfate standard solution consumed in the sample determination; D - the fraction, i.e. 250 / 10 = 25; m - the mass of the sample taken; 0.003 - the mass of carbon equivalent to 1.00 mL of ferrous sulfate standard solution with a concentration of 1.000 mol / L, in grams (g); f - the carbon content of DOM, which is 0.4.

[0117] Note to all technical personnel: Although the present invention has been described according to the above specific embodiments, the inventive idea of the present invention is not limited to this invention, and any modification using the inventive idea will be included in the scope of protection of the present patent.

Claims

1. A small molecule organic carbon water-soluble fertilizer for promoting eucalyptus lignification, characterized in that, The composition of the small-molecule organic carbon water-soluble fertilizer for promoting eucalyptus lignification comprises, by weight fraction, 3-5 parts of biological agent, 10-20 parts of food industry waste, 20-30 parts of organic solid fertilizer, 5-15 parts of sodium silicate, 10-20 parts of calcium dihydrogen phosphate, 5-15 parts of potassium chloride, 5-15 parts of magnesium nitrate, 5-15 parts of sulfur-coated urea, 0.1-0.5 parts of rooting agent, 0.5-1 part of trace element composite nutrient solution, 5-50 parts of deionized water and 1-3 parts of pH regulator; the biological agent is obtained by fermentation of a new Bacillus tequilensis strain XD63Y, which has a preservation number of GDMCC No: 62521; the preparation steps of the organic solid fertilizer are as follows: collecting plant straws, crushing the plant straws to a particle size of less than 5 mm, and composting or fermenting the crushed plant straws; during the composting process, the moisture content of the compost is kept at 50-60%, and the compost is turned over once every 3 days; the fermentation time is 30-45 days, and the temperature is controlled at 50-60 DEG C; after the fermentation is completed, the compost product is collected, dried and crushed to a particle size of less than 1 mm to obtain the organic solid fertilizer rich in organic nutrients.

2. A small molecular organic carbon water-soluble fertilizer for promoting eucalyptus lignification according to claim 1, characterized in that, The composition of the food industry waste comprises, by weight fraction, 5-20 parts of distiller's grains concentrated waste liquid, 5-20 parts of monosodium glutamate concentrated waste liquid and 5-20 parts of soy sauce concentrated waste liquid; the organic matter content of the distiller's grains concentrated waste liquid is 45-60 wt%, the organic matter content of the monosodium glutamate concentrated waste liquid is 40-60 wt%, and the organic matter content of the soy sauce concentrated waste liquid is 40-60 wt%.

3. The small molecular organic carbon water-soluble fertilizer for promoting eucalyptus lignification according to claim 1, characterized in that, The trace element composite nutrient solution comprises, by weight fraction, 0.01-0.1 part of chelated iron (EDTA-Fe), 0.01-0.05 part of zinc nitrate (Zn(NO3)2), 0.005-0.02 part of copper nitrate (Cu(NO3)2), 0.01-0.05 part of manganese nitrate (Mn(NO3)2), 0.001-0.01 part of sodium molybdate (Na2MoO4) and 0.01-0.05 part of boric acid (H3BO3).

4. The small molecular organic carbon water-soluble fertilizer for promoting eucalyptus lignification according to claim 1, characterized in that, The pH regulator is potassium dihydrogen phosphate and potassium hydrogen phosphate.

5. The small molecular organic carbon water-soluble fertilizer for promoting eucalyptus lignification according to claim 1, characterized in that, The plant straws are one or more of wheat straw, cotton straw and corn straw.

6. A small molecular organic carbon water-soluble fertilizer for promoting eucalyptus lignification according to claim 1, characterized in that, The rooting agent is indole acetic acid.

7. The small-molecule organic carbon water-soluble fertilizer for promoting eucalyptus lignification according to any one of claims 1 to 5, which is in the form of a paste or a liquid, and a preparation method of the paste fertilizer, characterized in that, The method comprises the following steps: S1 inoculating Bacillus tequilensis XD63Y into a culture medium, and slowly shaking the flask at 40-50 DEG C and 10-30 rounds per minute in a constant-temperature water bath shaker for 24-96 hours; wherein the culture medium comprises 500 ml of distilled water, 5-40% of glucose, 0.3-3% of yeast extract, 0.5-5% of N, 0.3-3% of P2O5, 0.1-2% of K2O, 0.05-1% of trace elements and pH 5.5-8.5; S2 composting or fermenting the crushed plant straws, keeping the moisture content of the compost at 50-60%, turning over the compost once every 3 days, fermenting for 30-45 days, controlling the temperature at 50-60 DEG C, drying and crushing the compost to a particle size of less than 1 mm after the fermentation is completed, and obtaining the organic solid fertilizer; S3 According to the formula, add Bacillus tectum metabolic product, food industry waste, organic solid fertilizer, trace element compound nutrient solution and deionized water in a multifunctional reaction kettle, adjust the temperature to 40-50℃, the stirring speed is 100-200 revolutions / minute, stir for 12-24 hours, and use pH regulator to keep the pH of the mixed solution at 6.0-7.5 during the period; S4 Add sodium silicate, calcium dihydrogen phosphate and potassium chloride in the reaction kettle, adjust the temperature to 30-40℃, the stirring speed is 100-200 revolutions / minute, continue to stir for 2-4 hours, cool to room temperature after standing, and get the organic small molecule water-soluble fertilizer; S5 Put the prepared paste into degradable biological film packaging, each bag weighs 200-1000g, and place it in a dry and cool place after packaging, avoiding direct sunlight and high temperature environment.

8. A method for the preparation of a liquid fertilizer, characterized in that, The following steps are included: S1 According to the formula, add Bacillus tectum metabolic product, food industry waste, trace element compound nutrient solution and deionized water in a multifunctional reaction kettle, adjust the temperature to 40-50℃, the stirring speed is 100-200 revolutions / minute, stir for 12-24 hours, and use pH regulator to keep the pH of the mixed solution at 6.0-7.5 during the period; S2 Put the prepared liquid fertilizer through a filter screen to remove solid impurities, seal and store it in a bottle, and place the prepared liquid fertilizer in a dry and cool place, avoiding direct sunlight and high temperature environment.

9. The small molecular organic carbon water-soluble fertilizer for promoting eucalyptus lignification according to claim 1, characterized in that, The small molecule organic carbon refers to an organic matter composed of 3 or fewer carbons, which can be rapidly decomposed by microorganisms into water and carbon dioxide, providing energy and carbon elements for the ecosystem.

10. The use of the small-molecule organic carbon water-soluble fertilizer for promoting the lignification of eucalyptus according to any one of claims 1 to 6, characterized in that, During the growth period of eucalyptus, apply once every 2-3 months.

11. The use of the small-molecule organic carbon water-soluble fertilizer for promoting the lignification of eucalyptus according to claim 7, characterized in that, After fertilization, regularly detect the growth status of eucalyptus, including tree height, stem diameter, leaf color and pest situation, evaluate the effect of fertilizer, and adjust the fertilization scheme according to the test results.

Images