Temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mouse

Abstract

The invention discloses a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice, and relates to the technical field of cell incubators. The device comprises a support, an incubator body is fixedly connected to the top of the support, and an electric temperature controller is fixedly connected to the top of the incubator body. By means of the linkage design of the rotating assembly and the inflation and deflation assembly, dynamic regulation and control of the culture environment are achieved, the rotating rod drives the culture frame to rotate at a low speed, cultured neuron spheres make uniform contact with nutrition and gas, and central necrosis caused by static culture is avoided; cOmixed gas is uniformly sprayed into the culture box, the problem that gas uniformity is damaged by traditional rotary culture is solved, a pure rotary mode or a rotary inflation and deflation mode can be switched through a double-shaft motor and a gear meshing mechanism, the requirements of different experiment stages are met, and the survival rate and function expression of dopaminergic neurons are remarkably improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of cell incubators, and particularly relates to a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice. BACKGROUND

[0002] A cell incubator is a device used for culturing cells, tissues or microorganisms in a controlled environment, and is widely used in the fields of biology, medical research and biotechnology, etc. The main function of the cell incubator is to provide constant temperature, humidity, gas composition and other environmental conditions for cells to simulate the natural environment in vivo, so as to promote the growth, division and reproduction of cells. The cell incubator is usually equipped with a temperature control system to maintain the appropriate temperature required by cells. The environment in the cell incubator must be kept sterile, so most incubators are also equipped with ultraviolet lamps, filters and other devices to inhibit the growth of bacteria and fungi and ensure the purity of cell culture.

[0003] The cell incubator in the prior art still has some problems in use, such as: when culturing dopaminergic neurons of Parkinson mice, a static culture environment is usually used, which cannot simulate the dynamic neural microenvironment in vivo, resulting in abnormal synaptic development and functional loss of neurons. Although a conventional CO2 incubator can maintain a basic gas environment, there is a significant temperature and gas concentration gradient in the incubator, especially for the culture of brain-like organs. Cell necrosis caused by lack of nutrition and hypoxia often occurs in the central region. In addition, the rotation culture and gas regulation system in the prior art are independent of each other. The rotation device often destroys the gas homogeneity in the incubator, and the gas exchange process may interfere with the mechanical stability of the culture, which cannot meet the dual requirements of mechanical stimulation and oxidative stress research of dopaminergic neurons in the Parkinson model. Moreover, the complex external gas system increases the risk of contamination.

[0004] Therefore, we provide a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice to solve the above problems. SUMMARY

[0005] The purpose of the present application is to provide a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice, which solves the problem of the cell incubator in the prior art, which is mostly a static culture environment, and the rotation culture device easily causes uneven gas concentration in some areas, resulting in internal cell hypoxia and necrosis.

[0006] To solve the above technical problems, the present application is realized by the following technical scheme.

[0007] The utility model provides a kind of temperature-controlled cell incubator for Parkinson mouse dopaminergic neuron culture, including support, the support top is fixedly connected with incubator body, and the incubator body top is fixedly connected with electric temperature controller;The support inner cavity is provided with inflation and deflation assembly, and the inflation and deflation assembly includes horizontal plate, and the horizontal plate is fixedly connected in support inner cavity one side, and the horizontal plate bottom is communicated with switching barrel, and the switching barrel inner cavity is slidably connected with piston rod, and the support inner cavity is slidably connected with gas source box, and the gas source box top one side is movably connected with reciprocating lead screw, and the reciprocating lead screw surface is threadedly connected with threaded plate, and the threaded plate inner cavity one side is fixedly connected with piston rod surface one side, and the switching barrel surface one side is communicated with air inlet pipe, and the air inlet pipe one end is communicated with gas source box top one side, and the air inlet pipe surface is equipped with solenoid valve, and the switching barrel surface other side is communicated with air outlet pipe, and the air outlet pipe one end is communicated with gas source box top one side, and the air outlet pipe surface is equipped with check valve, and the incubator body inner cavity both sides are fixedly connected with electrochemical sensor;The incubator body inner cavity is provided with rotating assembly, and the rotating assembly includes rotating shaft, and the rotating shaft is movably connected in incubator body inner cavity, and the rotating shaft one end is fixedly connected with culture rack, and the culture rack top all around is equipped with culture groove, and the horizontal plate inner cavity one side is fixedly connected with double-shaft motor, and the double-shaft motor one end is fixedly connected with first transmission rod, and the first transmission rod one end is rotatably connected with support inner top one side by pivot, and the first transmission rod surface is fixedly connected with first driving gear, and the rotating shaft surface one side is fixedly connected with first driven gear, and the first driving gear is engaged with first driven gear.

[0008] The utility model further provides that the horizontal plate bottom one side is fixedly connected with limiting frame, and the double-shaft motor one end is fixedly connected with second transmission rod, and the second transmission rod one end is rotatably connected with the limiting frame inner bottom by pivot.

[0009] The utility model further provides that the second transmission rod surface is fixedly connected with second driving gear, and the reciprocating lead screw surface one side is fixedly connected with second driven gear, and the reciprocating lead screw surface other side is fixedly connected with third driven gear.

[0010] The utility model further provides that the limiting frame one side is slidably connected with support plate, and the support plate bottom is rotatably connected with reciprocating lead screw one end by pivot.

[0011] The utility model further provides that the horizontal plate top is communicated with air supply pipe, and the air supply pipe one end is communicated with switching barrel one end, and the air supply pipe other end is communicated with rotating seal joint with rotating shaft one end.

[0012] The utility model further provides that the piston rod one end extends to the inside of gas source box, and the piston rod one end is fixedly connected with extrusion plate, and the extrusion plate surface is slidably connected with gas source box inner cavity.

[0013] The gas source box is further provided with a convex frame on both sides, a limiting groove is formed around the inner cavity of the support, and the convex frame is slidably connected to the limiting groove.

[0014] The gas source box is further provided with a convex frame on both sides, a limiting groove is formed around the inner cavity of the support, and the convex frame is slidably connected to the limiting groove.

[0015] The gas source box is further provided with a convex frame on both sides, a limiting groove is formed around the inner cavity of the support, and the convex frame is slidably connected to the limiting groove.

[0016] The gas source box is further provided with a convex frame on both sides, a limiting groove is formed around the inner cavity of the support, and the convex frame is slidably connected to the limiting groove.

[0017] The gas source box is further provided with a convex frame on both sides, a limiting groove is formed around the inner cavity of the support, and the convex frame is slidably connected to the limiting groove.

[0018] 1. The present application realizes dynamic regulation of the culture environment through the linkage design of the rotating assembly and the gas charging and discharging assembly. The rotating shaft drives the culture rack to rotate at a low speed, so that the cultured neuron spheres uniformly contact nutrients and gas, avoiding central necrosis caused by static culture. Meanwhile, the multiple gas holes in the rotating shaft uniformly spray CO2 mixed gas into the incubator through the piston type gas charging and discharging system, solving the problem of destruction of gas uniformity in traditional rotary culture. The double-shaft motor and the gear meshing mechanism can switch between pure rotation and rotation plus gas charging and discharging modes, meeting the needs of different experimental stages and significantly improving the survival rate and functional expression of dopaminergic neurons.

[0019] 2. The present application realizes precise circulation of gas in the incubator through the synergistic effect of the gas source box, the switching cylinder and the electromagnetic valve. The electrochemical sensor monitors the concentrations of CO2 and O2 in real time, and feedback controls the opening and closing of the electromagnetic valve and the lifting of the gas cylinder, so that the gas concentration fluctuation is small. The reciprocating motion of the piston rod is driven by the reciprocating screw, ensuring that there is no pulse interference in the gas charging and discharging process, avoiding the negative impact of traditional air pump vibration on neuron synapse growth, and eliminating the risk of external pollution through closed gas path design. It is especially suitable for long-term culture of sensitive neurons in Parkinson's disease models.

[0020] 3. The present application adopts a split design for the incubator, so that the gas source box and the rotating assembly are slidably connected through the limiting groove and the convex frame, which is convenient for disassembly, sterilization or replacement of gas mixing ratio. The lifting mechanism driven by the gas cylinder can quickly switch the gas charging and discharging state, reducing manual intervention. The multi-slot design of the culture rack is compatible with standard culture dishes and microcarriers, expanding the experimental application scenarios. The device has bionics, stability and repeatability in Parkinson's disease research, providing an efficient tool for mechanism research and drug screening of neurodegenerative diseases.

[0021] Of course, implementing any product of the application does not necessarily require achieving all the advantages described above at the same time. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly introduced as follows.

[0023] Figure 1 It is a stereogram of a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice.

[0024] Figure 2 It is a sectional view of the incubator body in a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice.

[0025] Figure 3 It is a sectional view of the bracket in a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice.

[0026] Figure 4 It is an exploded view of the air cylinder and the convex bracket in a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice.

[0027] Figure 5 It is a schematic view of the surface structure of the cross plate in a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice.

[0028] Figure 6 It is an exploded view of the internal structure of the switching cylinder in a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice.

[0029] Figure 7 It is an exploded view of the surface structure of the limiting bracket in a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice.

[0030] Figure 8 It is an exploded view of the support plate and the reciprocating lead screw in a temperature-controlled cell incubator for culturing dopaminergic neurons of Parkinson mice.

[0031] In the drawings: 1, bracket; 2, incubator body; 3, electric temperature controller; 4, cross plate; 5, switching cylinder; 6, piston rod; 7, air source box; 8, reciprocating lead screw; 9, threaded plate; 10, air inlet pipe; 11, electromagnetic valve; 12, air outlet pipe; 13, one-way valve; 14, electrochemical sensor; 15, rotating rod; 16, culture rack; 17, culture tank; 18, double-shaft motor; 19, first transmission rod; 20, first driving gear; 21, first driven gear; 22, limiting bracket; 23, second transmission rod; 24, second driving gear; 25, second driven gear; 26, third driven gear; 27, support plate; 28, air feeding pipe; 29, extrusion plate; 30, convex bracket; 31, limiting groove; 32, air cylinder; 33, limiting rod; 34, air hole. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present application will be described below with reference to the drawings in the embodiments of the present application. The described embodiments are only some of the embodiments of the present application, not all.

[0033] Example 1

[0034] Please refer to Figures 1-8The utility model provides a kind of temperature-controlled cell incubator for Parkinson mouse dopaminergic neuron culture, including support 1, support 1 top fixedly connected with incubator 2, incubator 2 one side is hingedly connected with door, door one side is fixedly connected with handle, door inner cavity is equipped with visual port, visual port inner cavity is fixedly connected with toughened glass, and the opening between door and incubator 2 is sealedly connected by sealing structure, incubator 2 top is fixedly connected with electric temperature controller 3, electric temperature controller 3 is existing structure, can carry out temperature and humidity control to incubator 2 inside;Support 1 inner cavity is provided with inflation and deflation assembly, inflation and deflation assembly includes horizontal plate 4, horizontal plate 4 is fixedly connected in support 1 inner cavity one side, horizontal plate 4 bottom is communicated with switching cylinder 5, switching cylinder 5 makes that air is admitted to incubator 2 inside, and air is exhausted from incubator 2 inside and is carried out in this cylinder, piston rod 6 is slidably connected in switching cylinder 5 inner cavity, piston rod 6 top end is sealingly slidably connected with switching cylinder 5 inner cavity, support 1 inner cavity is slidably connected with gas source tank 7, gas source tank 7 one side top is communicated with gas injection pipe, valve is installed on the surface of gas injection pipe, to facilitate the inside of gas source tank 7 to supplement mixed gas or discharge excess gas, so that the gas ratio inside gas source tank 7 reaches standard state, gas source tank 7 top one side is movably connected with reciprocating lead screw 8, reciprocating lead screw 8 surface is threadedly connected with threaded plate 9, threaded plate 9 is used to drive piston rod 6 to reciprocate within a certain range, threaded plate 9 inner cavity one side is fixedly connected with piston rod 6 surface one side, switching cylinder 5 surface one side is communicated with air inlet pipe 10, when second driving gear 24 is engaged with second driven gear 25, piston rod 6 is moved by reciprocating lead screw 8, can use air inlet pipe 10 to pump from the inside of gas source tank 7 to gas hole 34, while second driving gear 24 is engaged with third driven gear 26, air outlet pipe 12 can discharge the gas in incubator body to the inside of gas source tank 7 by one-way valve 13, air inlet pipe 10 one end is communicated with gas source tank 7 top one side, electromagnetic valve 11 is installed on the surface of air inlet pipe 10, when second driving gear 24 is engaged with third driven gear 26, electromagnetic valve 11 closes air inlet pipe 10, switching cylinder 5 surface other side is communicated with air outlet pipe 12, air outlet pipe 12 one end is communicated with gas source tank 7 top one side, one-way valve 13 is installed on the surface of air outlet pipe 12, one-way valve 13 makes that the gas in switching cylinder 5 can be transported to gas source tank 7, and gas in gas source tank 7 will not be discharged to switching cylinder 5, incubator 2 inner cavity both sides are fixedly connected with electrochemical sensor 14, electrochemical sensor 14 is existing structure, for detecting the gas change in incubator 2 inside, not described here again.The inner cavity of the incubator body 2 is provided with a rotating assembly, the rotating assembly comprises a rotating rod 15, one end of the rotating rod 15 extends to the outside of the incubator body 2, and the connection is sealed by a sealing structure, the rotating rod 15 is movably connected in the inner cavity of the incubator body 2, one end of the rotating rod 15 is fixedly connected with a culture rack 16, the culture rack 16 is provided with culture grooves 17 around the top, and the plurality of culture grooves 17 on the top of the culture rack 16 are used for placing a plurality of culture dishes, so that a plurality of cells can be cultured at the same time, a double-shaft motor 18 is fixedly connected on one side in the inner cavity of the horizontal plate 4, one end of the double-shaft motor 18 is fixedly connected with a first transmission rod 19, one end of the first transmission rod 19 is rotatably connected with the inner top of the support 1 on one side, a first driving gear 20 is fixedly connected on the surface of the first transmission rod 19, the first driving gear 20 is meshed with a first driven gear 21, so that the double-shaft motor 18 can drive the rotating rod 15 to rotate, one side of the surface of the rotating rod 15 is fixedly connected with the first driven gear 21, and the first driving gear 20 is meshed with the first driven gear 21.

[0035] Example 2

[0036] Please refer to Figures 1-8 On the basis of example 1, the bottom of the horizontal plate 4 is fixedly connected with a limiting frame 22, the limiting frame 22 is hollow around, so that the second driving gear 24 is meshed with the second driven gear 25 or the third driven gear 26, one end of a second transmission rod 23 is fixedly connected with the double-shaft motor 18, one end of the first transmission rod 19 and the second transmission rod 23 is respectively fixedly connected with the two output ends of the double-shaft motor 18, one end of the second transmission rod 23 is rotatably connected with the inner bottom of the limiting frame 22, a second driving gear 24 is fixedly connected on the surface of the second transmission rod 23, a second driven gear 25 is fixedly connected on one side of the surface of the reciprocating lead screw 8, a third driven gear 26 is fixedly connected on the other side of the surface of the reciprocating lead screw 8, the second driving gear 24 is meshed with the second driven gear 25, so that the piston rod 6 can move to intake air in the incubator body 2, when the second driving gear 24 is meshed with the third driven gear 26, the piston rod 6 can drive the incubator body 2 to exhaust air, when the second driving gear 24 is located between the second driven gear 25 and the third driven gear 26 and is not meshed with the two, the double-shaft motor 18 only drives the rotating rod 15 to rotate, a supporting plate 27 is slidably connected on one side of the limiting frame 22, limiting blocks are fixedly connected on the two sides of the supporting plate 27, limiting openings are formed in the two sides of the limiting frame 22, so as to support and limit the supporting plate 27, the bottom of the supporting plate 27 is rotatably connected with one end of the reciprocating lead screw 8, a gas supply pipe 28 is communicated with the top of the horizontal plate 4, one end of the gas supply pipe 28 is communicated with one end of the switching cylinder 5, the other end of the gas supply pipe 28 is communicated with one end of the rotating rod 15 through a rotary sealing joint, the existing rotary sealing joint is used to make the rotating rod 15 rotate without driving the switching cylinder 5 to rotate, and the two can be sealed and communicated.

[0037] Example 3

[0038] Please refer toFigures 1-8 On the basis of embodiment 1 and embodiment 2, the piston rod 6 extends to the inside of the gas source box 7 at one end, and the piston rod 6 is fixedly connected with the extrusion plate 29 at one end; the reciprocating movement of the piston rod 6 can extrude the internal gas by the extrusion plate 29, so that the air inlet pipe 10 or the air outlet pipe 12 can discharge the gas in the gas source box 7 or suck the external gas into the gas source box 7; the surface of the extrusion plate 29 is in sliding connection with the inner cavity of the gas source box 7; the convex frame 30 is fixedly connected on both sides of the gas source box 7; the limiting grooves 31 are formed in the inner cavity of the support 1 around; the convex frame 30 and the limiting grooves 31 can support and limit the lifting of the gas source box 7; the convex frame 30 is in sliding connection with the inner cavity of the limiting groove 31 at one side; the air cylinders 32 are fixedly connected on both sides of the top of the support 1; the free end of the air cylinder 32 is fixedly connected with the top of one side of the convex frame 30; the limiting rod 33 is fixedly connected with the bottom of one side of the support plate 27; the limiting rod 33 is used for limiting the threaded plate 9; the limiting rod 33 is fixedly connected with the top of one side of the gas source box 7 at one end; the surface of the limiting rod 33 is in sliding connection with one side of the inner cavity of the threaded plate 9; the air holes 34 are formed in the inner cavity of the rotating rod 15 around; the inner cavity of the air hole 34 is in communication with the inside of the switching barrel 5 through the inner cavity of the air supply pipe 28.

[0039] The working principle of the application is as follows: after the incubator is started, the electric temperature controller 3 first stabilizes the temperature in the incubator body 2 in a certain range and maintains the humidity in a suitable range; the double-shaft motor 18 is started through an external controller; the double-shaft motor 18 works to drive the first driving gear 20 and the first driven gear 21 to engage through the first transmission rod 19, drive the rotating rod 15 and the incubator rack 16 to rotate stably and slowly, and make the neuron spheres in the culture tank 17 uniformly contact the culture medium, so as to avoid the gradient effect of static culture; when the electrochemical sensor 14 detects that the CO2 and O2 concentrations deviate from the set values.

[0040] The system starts the gas regulation process. If the gas needs to be supplemented, the air cylinder 32 pushes the gas source box 7 to rise, so that the second driving gear 24 is engaged with the second driven gear 25. At this time, the reciprocating movement area of the top end of the piston rod 6 is at the bottom of the one side of the inlet pipe 10 and at the top of the outlet pipe 12 in the switching cylinder 5. The double-shaft motor 18 drives the reciprocating screw 8 to rotate through the second driving gear 24 and the second driven gear 25 engaged with the second transmission rod 23, so as to drive the threaded plate 9 and the piston rod 6 to reciprocate. The reciprocating extrusion plate 29 at one end of the piston rod 6 extrudes the inside of the gas source box 7, so that a negative pressure is formed in the switching cylinder 5. The mixed gas in the gas source box 7 enters the switching cylinder 5 through the inlet pipe 10 of the opened electromagnetic valve 11, and then is transported to the inside of the rotating rod 15 through the gas conveying pipe 28, and finally is uniformly sprayed from the air hole 34. If the gas needs to be discharged, the air cylinder 32 pulls down the gas source box 7 to make the second driving gear 24 engaged with the third driven gear 26. The piston rod 6 moves down to the bottom of the one side of the outlet pipe 12 in the switching cylinder 5, and reciprocates in this area to press the gas in the box back to the gas source box 7 through the outlet pipe 12, and prevent the gas from flowing back through the one-way valve 13. At the same time, the electromagnetic valve 11 closes the inlet pipe 10. In this process, the rotary sealing joint ensures that the rotating rod 15 does not leak when rotating. The limiting rod 33 and the convex frame 30 cooperate to maintain the stability of the lifting of the gas source box 7. Through the alternately executed rotating, inflating and discharging actions, the system dynamically maintains the concentration of O2 and CO2 in the box and the accurate balance of temperature and humidity, and runs in a closed mode throughout to eliminate the risk of pollution.

[0041] The above only describes some exemplary embodiments of the present application by way of illustration. It is needless to say that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present application. Therefore, the above figures and descriptions are illustrative in nature and should not be understood as limiting the scope of the claims of the present application.

Claims

1. A temperature-controlled cell incubator for culturing of dopaminergic neurons of Parkinson mice, comprising a support (1), characterized in that: The top of the support (1) is fixedly connected with a culture box body (2), and the top of the culture box body (2) is fixedly connected with an electric temperature controller (3); The inner cavity of the support (1) is provided with a gas charging and discharging assembly, the gas charging and discharging assembly comprises a horizontal plate (4), one side of the inner cavity of the horizontal plate (4) is fixedly connected with the support (1), the bottom of the horizontal plate (4) is communicated with a switching cylinder (5), the inner cavity of the switching cylinder (5) is slidably connected with a piston rod (6), the inner cavity of the support (1) is slidably connected with a gas source box (7), one side of the top of the gas source box (7) is movably connected with a reciprocating lead screw (8), the surface of the reciprocating lead screw (8) is threadedly connected with a threaded plate (9), one side of the inner cavity of the threaded plate (9) is fixedly connected with one side of the surface of the piston rod (6), one side of the surface of the switching cylinder (5) is communicated with an air inlet pipe (10), one end of the air inlet pipe (10) is communicated with one side of the top of the gas source box (7), the surface of the air inlet pipe (10) is mounted with a solenoid valve (11), the other side of the surface of the switching cylinder (5) is communicated with an air outlet pipe (12), one end of the air outlet pipe (12) is communicated with one side of the top of the gas source box (7), the surface of the air outlet pipe (12) is mounted with a check valve (13), and the inner cavities of the culture box body (2) are fixedly connected with electrochemical sensors (14) on both sides. The inner cavity of the culture box body (2) is provided with a rotating assembly, the rotating assembly comprises a rotating rod (15), the rotating rod (15) is movably connected in the inner cavity of the culture box body (2), one end of the rotating rod (15) is fixedly connected with a culture rack (16), culture grooves (17) are formed in the top of the culture rack (16) around, one side of the inner cavity of the horizontal plate (4) is fixedly connected with a double-shaft motor (18), one end of the double-shaft motor (18) is fixedly connected with a first transmission rod (19), one end of the first transmission rod (19) is rotatably connected with one side of the inner top of the support (1) through a rotating shaft, the surface of the first transmission rod (19) is fixedly connected with a first driving gear (20), one side of the surface of the rotating rod (15) is fixedly connected with a first driven gear (21), and the first driving gear (20) is engaged with the first driven gear (21).

2. The temperature-controlled cell incubator for culturing of dopaminergic neurons of Parkinson mice according to claim 1, characterized in that: One side of the bottom of the horizontal plate (4) is fixedly connected with a limiting frame (22), one end of the double-shaft motor (18) is fixedly connected with a second transmission rod (23), and one end of the second transmission rod (23) is rotatably connected with the inner bottom of the limiting frame (22) through a rotating shaft.

3. The temperature-controlled cell culture incubator for culturing of dopaminergic neurons of Parkinson mice according to claim 2, characterized in that: The surface of the second transmission rod (23) is fixedly connected with a second driving gear (24), one side of the surface of the reciprocating lead screw (8) is fixedly connected with a second driven gear (25), and the other side of the surface of the reciprocating lead screw (8) is fixedly connected with a third driven gear (26).

4. The temperature-controlled cell culture incubator for culturing of dopaminergic neurons of Parkinson mice according to claim 2, characterized in that: One side of the limiting frame (22) is slidably connected with a supporting plate (27), and the bottom of the supporting plate (27) is rotatably connected with one end of the reciprocating lead screw (8) through a rotating shaft.

5. The temperature-controlled cell culture incubator for culturing of dopaminergic neurons of Parkinson mice according to claim 1, characterized in that: The top of the horizontal plate (4) is communicated with a gas feeding pipe (28), one end of the gas feeding pipe (28) is communicated with one end of the switching cylinder (5), and the other end of the gas feeding pipe (28) is communicated with one end of the rotating rod (15) through a rotary sealing joint.

6. The temperature-controlled cell culture incubator for culturing of dopaminergic neurons of Parkinson mice according to claim 1, characterized in that: The piston rod (6) extends to the inside of the gas source box (7), one end of the piston rod (6) is fixedly connected with the extrusion plate (29), the surface of the extrusion plate (29) is slidably connected with the inner cavity of the gas source box (7).

7. The temperature-controlled cell culture incubator for culturing of dopaminergic neurons of Parkinson mice according to claim 1, characterized in that: The gas source box (7) is fixedly connected with the convex frame (30) on both sides, the inner cavity of the support (1) is provided with the limiting groove (31) around, and the convex frame (30) is slidably connected with the inner cavity of the limiting groove (31) on one side.

8. The temperature-controlled cell culture incubator for culturing of dopaminergic neurons of Parkinson mice according to claim 1, characterized in that: The inner top of the support (1) is fixedly connected with the air cylinder (32) on both sides, and the free end of the air cylinder (32) is fixedly connected with one side of the top of the convex frame (30).

9. The temperature-controlled cell culture incubator for culturing of dopaminergic neurons of Parkinson mice according to claim 4, characterized in that: The bottom of the supporting plate (27) is fixedly connected with the limiting rod (33) on one side, one end of the limiting rod (33) is fixedly connected with one side of the top of the gas source box (7), and the surface of the limiting rod (33) is slidably connected with one side of the inner cavity of the threaded plate (9).

10. The temperature-controlled cell culture incubator for culturing of dopaminergic neurons of Parkinson mice according to claim 1, characterized in that: The inner cavity of the rotating rod (15) is provided with the air hole (34) around, and the inner cavity of the air hole (34) is connected with the inside of the switching barrel (5) through the inner cavity of the air pipe (28).

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