Particle composition derived from plant sterilization and disinfection and preparation process thereof

Abstract

The invention relates to the technical field of plant sterilization and disinfection, in particular to a granular composition derived from plant sterilization and disinfection and a preparation process thereof. The particle composition is prepared from the following raw materials in parts by weight: 2 to 3 parts of chelating agent GL47, 8 to 10 parts of fatty acid, 5 to 6 parts of oleic acid, 12 to 15 parts of iso-tridecanol polyoxyethylene ether TO8, 10 to 12 parts of alkyl glycoside APG, 6 to 8 parts of disodium cocoyl glutamate, 1.5 to 2 parts of citric acid, 0.5 to 1 part of protease P300, 2 to 3 parts of seven-enzyme compound enzyme and 3.5 to 4.7 parts of modified hydroxypropyl methyl cellulose-plant extract. The origanum vulgare leaf extract, the garlic extract and the dandelion extract are adopted to form a plant sterilization and disinfection component, the sterilization effect is good, and the problem that the bioavailability is low due to the fact that the origanum vulgare leaf extract, the garlic extract and the dandelion extract serve as sterilization materials and are poor in water solubility is effectively solved.

Description

Technical Field

[0001] This invention relates to the field of plant sterilization and disinfection technology, specifically to a granular composition derived from plant sterilization and disinfection and its preparation process. Background Technology

[0002] Plant-derived bactericides and disinfectants have garnered significant attention in agriculture, medicine, and daily chemical industries due to their natural, low-toxicity, and environmentally friendly properties. Oregano leaf extract, garlic extract, and dandelion extract, as typical plant-based active ingredients, possess broad-spectrum antibacterial effects (e.g., oregano leaf extract can disrupt microbial cell membranes, allicin inhibits sulfhydryl enzyme activity, and taraxacin interferes with metabolic pathways).

[0003] Among them, the solubility of carvacrol contained in oregano leaf extract is 0.1 g / L, which cannot effectively contact pathogens in the aqueous phase; the solubility of allicin contained in allicin extract is 2.3 g / L, which is insufficient to release the minimum inhibitory concentration; and taraxacin contained in dandelion extract has even lower solubility, relying only on surface adsorption for sterilization. Therefore, plant-derived bactericides and disinfectants generally suffer from poor water solubility, resulting in low bioavailability. Summary of the Invention

[0004] The purpose of this invention is to provide a plant-derived bactericidal and disinfectant granular composition and its preparation process. The technical problem solved by this invention is that plant-derived bactericidal and disinfectant agents generally have poor water solubility, resulting in low bioavailability.

[0005] The objective of this invention can be achieved through the following technical solutions:

[0006] A process for preparing a plant-derived bactericidal and disinfectant granular composition includes the following steps:

[0007] Fatty acids, oleic acid, and isomeric tridecyl alcohol polyoxyethylene ether TO8 were mixed at 60°C for 20 minutes to obtain the first phase.

[0008] Deionized water, alkyl glycoside APG, and disodium cocoyl glutamate were mixed at 50°C for 10 min to obtain the second phase; the amount of deionized water used was twice the mass of alkyl glycoside APG08.

[0009] The chelating agent GL47, citric acid, protease P300, modified hydroxypropyl methylcellulose-plant extract, first phase and second phase were stirred at 55℃ for 30 min to obtain a mixed slurry.

[0010] The mixed slurry is granulated to obtain a granular composition derived from plant-based sterilization and disinfection.

[0011] As a further aspect of the present invention: a method for preparing modified hydroxypropyl methylcellulose-plant extract, comprising the following steps:

[0012] Step 1: Under nitrogen protection, methyl methacrylate is mixed with ethyl 2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]acrylate, and azobisisobutyronitrile is added simultaneously. The mixture is reacted at a temperature of 65-80℃ to obtain UV-resistant modified methyl methacrylate.

[0013] 2-Ethyl isocyanate methacrylate was added to UV-resistant modified methyl methacrylate and reacted at 75°C to obtain end-group activated UV-resistant modified methyl methacrylate.

[0014] Step 2: Dissolve hydroxypropyl methylcellulose in anhydrous DMF, then add end-group activated UV-resistant modified methyl methacrylate, and react at 70-75℃ for 3 hours to obtain the modified hydroxypropyl methylcellulose carrier.

[0015] Step 3: Modified hydroxypropyl methylcellulose is combined with oregano leaf extract, garlic extract and dandelion extract respectively through intermolecular hydrogen bonding to obtain modified hydroxypropyl methylcellulose-plant extract.

[0016] As a further aspect of the present invention, the molar ratio of methyl methacrylate and ethyl 2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]acrylate is controlled to be 5:1.

[0017] As a further aspect of the present invention, the mass ratio of hydroxypropyl methylcellulose to anhydrous DMF is 10-15:100-150.

[0018] As a further aspect of the present invention, the molar ratio of hydroxypropyl methylcellulose to end-group activated UV-resistant modified methyl methacrylate is 3.5-4:1.

[0019] As a further aspect of the present invention: the reaction process of modified hydroxypropyl methylcellulose with oregano leaf extract is as follows:

[0020] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred, then oregano leaf extract was added. The mixture was reacted at pH 5.0-6.5 and temperature 25-35℃ to obtain modified hydroxypropyl methylcellulose-oregano leaf extract.

[0021] As a further aspect of the present invention: the reaction process of modified hydroxypropyl methylcellulose with garlic extract is as follows:

[0022] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred, then activated garlic extract was added, and the mixture was reacted at pH 6-7 and temperature 25-35℃ to obtain modified hydroxypropyl methylcellulose-garlic extract.

[0023] As a further aspect of the present invention: the reaction process of modified hydroxypropyl methylcellulose and dandelion extract is as follows:

[0024] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred, followed by the addition of activated dandelion extract. The mixture was reacted at pH 6-7 and temperature 25-35℃ to obtain modified hydroxypropyl methylcellulose-dandelion extract.

[0025] A granular composition derived from plant-based sterilization and disinfection, comprising the following raw materials in parts by weight:

[0026] 2-3 parts chelating agent GL47, 8-10 parts fatty acids, 5-6 parts oleic acid, 12-15 parts isomeric tridecyl polyoxyethylene ether TO8, 10-12 parts alkyl glycoside APG, 6-8 parts disodium cocoyl glutamate, 1.5-2 parts citric acid, 0.5-1 part protease P300, 2-3 parts hepta-enzyme complex, 3.5-4.7 parts modified hydroxypropyl methylcellulose-plant extract.

[0027] As a further embodiment of the present invention: the modified hydroxypropyl methylcellulose-plant extract comprises the following raw materials in parts by weight: 1.5-2 parts modified hydroxypropyl methylcellulose-oregano leaf extract, 1-1.5 parts modified hydroxypropyl methylcellulose-garlic extract and 1-1.2 parts modified hydroxypropyl methylcellulose-dandelion extract.

[0028] The beneficial effects of this invention are:

[0029] (1) The present invention uses oregano leaf extract, garlic extract and dandelion extract to form a plant bactericidal and disinfecting component, which has a good bactericidal effect. The three have the following synergistic bactericidal and disinfecting effects: oregano leaf extract can destroy the cell membrane phospholipid bilayer and promote allicin to enter the cell, while garlic extract can inhibit sulfhydryl enzyme activity and enhance dandelion acid metabolism interference, and dandelion extract can block sterol synthesis and weaken the microbial membrane repair ability.

[0030] (2) This invention modifies plant extracts with hydroxypropyl methylcellulose, which highly disperses drug molecules, breaks the original crystal structure, and forms an amorphous state with higher energy. In this state, the molecules are disordered, increasing surface free energy and significantly improving the solubility and dissolution rate of the drug in water. The hydroxypropyl methylcellulose polymer binds to the drug through intermolecular hydrogen bonds, preventing the drug molecules from rearranging and crystallizing during drying and storage, thus maintaining the stability of the amorphous state. Furthermore, hydroxypropyl methylcellulose contains hydrophilic hydroxyl groups and ether bonds, which rapidly hydrate upon contact with water to form a gel layer, enhancing the wettability of drug particles. Simultaneously, its water solubility promotes the dissolution of the gel layer, continuously releasing drug molecules. This effectively solves the problem of low bioavailability caused by the poor water solubility of oregano leaf extract, garlic extract, and dandelion extract as bactericidal materials.

[0031] (3) Furthermore, the present invention uses methyl methacrylate and hydroxypropyl methylcellulose for free radical graft copolymerization. The hydrophobicity of the methyl methacrylate side chain shields the ether bond of the hydroxypropyl methylcellulose main chain, which significantly reduces the risk of thermal oxidative degradation of hydroxypropyl methylcellulose modified plant extract at high temperature and increases the viscosity retention rate by more than 40%.

[0032] (4) In addition, during the grafting process of methyl methacrylate and hydroxypropyl methylcellulose, an ultraviolet absorbing group (ethyl 2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]acrylate) is introduced. This group preferentially absorbs ultraviolet light and converts it into heat energy, thereby blocking the photolytic reaction of the ether bond of hydroxypropyl methylcellulose. It also effectively solves the problem that methyl methacrylate is prone to chain breakage under ultraviolet irradiation, which leads to a decrease in molecular weight and reduces the stability of the bactericidal component in slow release. Detailed Implementation

[0033] To enable those skilled in the art to better understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

[0034] Example 1

[0035] This invention provides a plant-derived bactericidal and disinfecting granular composition, which comprises the following raw materials in parts by weight:

[0036] 2 parts chelating agent GL47, 8 parts fatty acid, 5 parts oleic acid, 12 parts isomeric tridecyl alcohol polyoxyethylene ether TO8, 10 parts alkyl glycoside APG, 6 parts disodium cocoyl glutamate, 1.5 parts citric acid, 0.5 parts protease P300, 2 parts hepta-enzyme complex enzyme, 3.5 parts modified hydroxypropyl methylcellulose-plant extract.

[0037] The modified hydroxypropyl methylcellulose-plant extract includes the following raw materials by weight: 1.5 parts modified hydroxypropyl methylcellulose-oregano leaf extract, 1 part modified hydroxypropyl methylcellulose-garlic extract, and 1 part modified hydroxypropyl methylcellulose-dandelion extract.

[0038] Specifically, the preparation method of modified hydroxypropyl methylcellulose-plant extract includes the following steps:

[0039] Step 1: Activate the end groups of UV-resistant modified methyl methacrylate:

[0040] Under nitrogen protection, methyl methacrylate was mixed with ethyl 2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]acrylate, and azobisisobutyronitrile was added simultaneously. The reaction was maintained at 65°C for 30 minutes, and then immediately cooled to 50°C to obtain UV-resistant modified methyl methacrylate.

[0041] 2-ethyl isocyanate methacrylate was added to UV-resistant modified methyl methacrylate and reacted at 75°C for 2 hours to obtain end-group activated UV-resistant modified methyl methacrylate.

[0042] The molar ratio of methyl methacrylate and ethyl 2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]acrylate is controlled to be 5:1, and the mass of azobisisobutyronitrile is 0.03 wt% of the mass of the reaction system in step 1.

[0043] Step 2: Preparation of modified hydroxypropyl methylcellulose carrier:

[0044] Hydroxypropyl methylcellulose was dissolved in anhydrous DMF, and then end-group activated UV-resistant modified methyl methacrylate was added. The mixture was reacted at 70°C for 3 hours to obtain the modified hydroxypropyl methylcellulose carrier.

[0045] The mass ratio of hydroxypropyl methylcellulose to anhydrous DMF is 10:100, and the molar ratio of hydroxypropyl methylcellulose to end-group activated UV-resistant modified methyl methacrylate is 3.5:1.

[0046] Step 3: Modified hydroxypropyl methylcellulose is combined with oregano leaf extract, garlic extract and dandelion extract respectively through intermolecular hydrogen bonding to obtain modified hydroxypropyl methylcellulose-plant extract;

[0047] In a first exemplary manner, the reaction process of combining modified hydroxypropyl methylcellulose with oregano leaf extract is as follows:

[0048] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred at 35°C for 1 hour. Then, oregano leaf extract was added and reacted at pH 5.0 and 25°C for 1 hour. After freeze drying, modified hydroxypropyl methylcellulose-oregano leaf extract was obtained.

[0049] The mass ratio of modified hydroxypropyl methylcellulose to distilled water is 8:100, and the molar ratio of modified hydroxypropyl methylcellulose to oregano leaf extract is 1:1.

[0050] A second exemplary embodiment describes the reaction process of modified hydroxypropyl methylcellulose combined with garlic extract as follows:

[0051] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred at 35°C for 1 hour. Then, activated garlic extract (garlic extract was dissolved in a 20% ethanol aqueous solution, and the concentration of garlic extract was controlled at 40 mg / mL) was added. After reacting at pH 6 and temperature 25°C for 1 hour, the modified hydroxypropyl methylcellulose-garlic extract was obtained by freeze drying.

[0052] The mass ratio of modified hydroxypropyl methylcellulose to distilled water is 8:100, and the molar ratio of modified hydroxypropyl methylcellulose to garlic extract is 1:1.

[0053] Thirdly, exemplarily, the reaction process of modified hydroxypropyl methylcellulose combined with dandelion extract is as follows:

[0054] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred at 35°C for 1 hour. Then, activated dandelion extract (dandelion extract dissolved in 20% ethanol aqueous solution, and garlic extract concentration controlled at 40 mg / mL) was added. After reacting at pH 6 and temperature 25°C for 1 hour, the modified hydroxypropyl methylcellulose-dandelion extract was obtained by freeze drying.

[0055] The mass ratio of modified hydroxypropyl methylcellulose to distilled water is 8:100, and the molar ratio of modified hydroxypropyl methylcellulose to dandelion extract is 1:1.

[0056] The present invention provides a process for preparing a plant-derived bactericidal and disinfecting granular composition, comprising the following steps:

[0057] Fatty acids, oleic acid, and isomeric tridecyl alcohol polyoxyethylene ether TO8 were mixed at 60°C for 20 minutes to obtain the first phase.

[0058] Deionized water, alkyl glycoside APG, and disodium cocoyl glutamate were mixed at 50°C for 10 min to obtain the second phase; the amount of deionized water used was twice the mass of alkyl glycoside APG08.

[0059] The chelating agent GL47, citric acid, protease P300, modified hydroxypropyl methylcellulose-plant extract, first phase and second phase were stirred at 55℃ for 30 min to obtain a mixed slurry.

[0060] The mixed slurry was granulated using a swing granulator, with the extrusion pressure controlled at 0.5 MPa, and then dried and cured to obtain a granular composition derived from plant sterilization and disinfection.

[0061] Example 2

[0062] This invention provides a plant-derived bactericidal and disinfecting granular composition, which comprises the following raw materials in parts by weight:

[0063] 2.5 parts chelating agent GL47, 9 parts fatty acids, 5.5 parts oleic acid, 13 parts isomeric tridecyl alcohol polyoxyethylene ether TO8, 11 parts alkyl glycoside APG, 7 parts disodium cocoyl glutamate, 1.8 parts citric acid, 0.8 parts protease P300, 2.5 parts hepta-enzyme complex, 4.1 parts modified hydroxypropyl methylcellulose-plant extract;

[0064] The modified hydroxypropyl methylcellulose-plant extract includes the following raw materials in parts by weight: 1.8 parts modified hydroxypropyl methylcellulose-oregano leaf extract, 1.2 parts modified hydroxypropyl methylcellulose-garlic extract and 1.1 parts modified hydroxypropyl methylcellulose-dandelion extract;

[0065] Specifically, the preparation method of modified hydroxypropyl methylcellulose-plant extract includes the following steps:

[0066] Step 1: Activate the end groups of UV-resistant modified methyl methacrylate:

[0067] Under nitrogen protection, methyl methacrylate was mixed with ethyl 2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]acrylate, and azobisisobutyronitrile was added simultaneously. The reaction was maintained at 70°C for 40 minutes, and then immediately cooled to 50°C to obtain UV-resistant modified methyl methacrylate.

[0068] 2-Ethyl isocyanate methacrylate was added to UV-resistant modified methyl methacrylate and reacted at 75°C for 2.5 hours to obtain end-group activated UV-resistant modified methyl methacrylate.

[0069] The molar ratio of methyl methacrylate and ethyl 2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]acrylate is controlled to be 5:1, and the mass of azobisisobutyronitrile is 0.2 wt% of the mass of the reaction system in step 1.

[0070] Step 2: Preparation of modified hydroxypropyl methylcellulose carrier:

[0071] Hydroxypropyl methylcellulose was dissolved in anhydrous DMF, and then end-group activated UV-resistant modified methyl methacrylate was added. The mixture was reacted at 73°C for 3 hours to obtain the modified hydroxypropyl methylcellulose carrier.

[0072] The mass ratio of hydroxypropyl methylcellulose to anhydrous DMF is 12:120, and the molar ratio of hydroxypropyl methylcellulose to end-group activated UV-resistant modified methyl methacrylate is 3.8:1.

[0073] Step 3: Modified hydroxypropyl methylcellulose is combined with oregano leaf extract, garlic extract and dandelion extract respectively through intermolecular hydrogen bonding to obtain modified hydroxypropyl methylcellulose-plant extract;

[0074] In a first exemplary manner, the reaction process of combining modified hydroxypropyl methylcellulose with oregano leaf extract is as follows:

[0075] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred at 38°C for 1 hour. Then, oregano leaf extract was added and reacted at pH 6.0 and 30°C for 1.5 hours. After freeze drying, modified hydroxypropyl methylcellulose-oregano leaf extract was obtained.

[0076] The mass ratio of modified hydroxypropyl methylcellulose to distilled water is 10:100, and the molar ratio of modified hydroxypropyl methylcellulose to oregano leaf extract is 1:1.5.

[0077] A second exemplary embodiment describes the reaction process of modified hydroxypropyl methylcellulose combined with garlic extract as follows:

[0078] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred at 38°C for 1 hour. Then, activated garlic extract (garlic extract was dissolved in a 20% ethanol aqueous solution, and the concentration of garlic extract was controlled at 40 mg / mL) was added. The reaction was carried out at pH 6 and temperature 30°C for 1.5 hours. After freeze drying, modified hydroxypropyl methylcellulose-garlic extract was obtained.

[0079] The mass ratio of modified hydroxypropyl methylcellulose to distilled water is 10:100, and the molar ratio of modified hydroxypropyl methylcellulose to garlic extract is 1:1.5.

[0080] Thirdly, exemplarily, the reaction process of modified hydroxypropyl methylcellulose combined with dandelion extract is as follows:

[0081] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred at 38°C for 1 hour. Then, activated dandelion extract (dandelion extract dissolved in 20% ethanol aqueous solution, and garlic extract concentration controlled at 40 mg / mL) was added. The reaction was carried out at pH 6 and temperature 30°C for 1.5 hours. After freeze drying, modified hydroxypropyl methylcellulose-dandelion extract was obtained.

[0082] The mass ratio of modified hydroxypropyl methylcellulose to distilled water is 10:100, and the molar ratio of modified hydroxypropyl methylcellulose to dandelion extract is 1:1.5.

[0083] The present invention provides a process for preparing a plant-derived bactericidal and disinfecting granular composition, comprising the following steps:

[0084] Fatty acids, oleic acid, and isomeric tridecyl alcohol polyoxyethylene ether TO8 were mixed at 60°C for 20 minutes to obtain the first phase.

[0085] Deionized water, alkyl glycoside APG, and disodium cocoyl glutamate were mixed at 50°C for 10 min to obtain the second phase; the amount of deionized water used was twice the mass of alkyl glycoside APG08.

[0086] The chelating agent GL47, citric acid, protease P300, modified hydroxypropyl methylcellulose-plant extract, first phase and second phase were stirred at 55℃ for 30 min to obtain a mixed slurry.

[0087] The mixed slurry was granulated using a swing granulator, with the extrusion pressure controlled at 0.5 MPa, and then dried and cured to obtain a granular composition derived from plant sterilization and disinfection.

[0088] Example 3

[0089] This invention provides a plant-derived bactericidal and disinfecting granular composition, which comprises the following raw materials in parts by weight:

[0090] 3 parts chelating agent GL47, 10 parts fatty acid, 6 parts oleic acid, 15 parts isomeric tridecyl alcohol polyoxyethylene ether TO8, 12 parts alkyl glycoside APG, 8 parts disodium cocoyl glutamate, 2 parts citric acid, 1 part protease P300, 3 parts hepta-enzyme complex enzyme, 4.7 parts modified hydroxypropyl methylcellulose-plant extract.

[0091] The modified hydroxypropyl methylcellulose-plant extract includes the following raw materials in parts by weight: 2 parts modified hydroxypropyl methylcellulose-oregano leaf extract, 1.5 parts modified hydroxypropyl methylcellulose-garlic extract and 1.2 parts modified hydroxypropyl methylcellulose-dandelion extract.

[0092] Specifically, the preparation method of modified hydroxypropyl methylcellulose-plant extract includes the following steps:

[0093] Step 1: Activate the end groups of UV-resistant modified methyl methacrylate:

[0094] Under nitrogen protection, methyl methacrylate was mixed with ethyl 2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]acrylate, and azobisisobutyronitrile was added simultaneously. The reaction was maintained at 80°C for 60 minutes, and then immediately cooled to 50°C to obtain UV-resistant modified methyl methacrylate.

[0095] 2-ethyl isocyanate methacrylate was added to UV-resistant modified methyl methacrylate and reacted at 75°C for 3 hours to obtain end-group activated UV-resistant modified methyl methacrylate.

[0096] The molar ratio of methyl methacrylate and ethyl 2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]acrylate is controlled to be 5:1, and the mass of azobisisobutyronitrile is 0.5 wt% of the mass of the reaction system in step 1.

[0097] Step 2: Preparation of modified hydroxypropyl methylcellulose carrier:

[0098] Hydroxypropyl methylcellulose was dissolved in anhydrous DMF, and then end-group activated UV-resistant modified methyl methacrylate was added. The mixture was reacted at 75°C for 3 hours to obtain the modified hydroxypropyl methylcellulose carrier.

[0099] The mass ratio of hydroxypropyl methylcellulose to anhydrous DMF is 15:150, and the molar ratio of hydroxypropyl methylcellulose to end-group activated UV-resistant modified methyl methacrylate is 4:1.

[0100] Step 3: Modified hydroxypropyl methylcellulose is combined with oregano leaf extract, garlic extract and dandelion extract respectively through intermolecular hydrogen bonding to obtain modified hydroxypropyl methylcellulose-plant extract;

[0101] In a first exemplary manner, the reaction process of combining modified hydroxypropyl methylcellulose with oregano leaf extract is as follows:

[0102] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred at 40°C for 1 hour. Then, oregano leaf extract was added and reacted at pH 6.5 and 35°C for 2 hours. After freeze drying, modified hydroxypropyl methylcellulose-oregano leaf extract was obtained.

[0103] The mass ratio of modified hydroxypropyl methylcellulose to distilled water is 12:100, and the molar ratio of modified hydroxypropyl methylcellulose to oregano leaf extract is 1:2.

[0104] A second exemplary embodiment describes the reaction process of modified hydroxypropyl methylcellulose combined with garlic extract as follows:

[0105] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred at 40°C for 1 hour. Then, activated garlic extract (garlic extract was dissolved in a 20% ethanol aqueous solution, and the concentration of garlic extract was controlled at 40 mg / mL) was added. After reacting at pH 7 and temperature 35°C for 2 hours, the modified hydroxypropyl methylcellulose-garlic extract was obtained by freeze drying.

[0106] The mass ratio of modified hydroxypropyl methylcellulose to distilled water is 12:100, and the molar ratio of modified hydroxypropyl methylcellulose to garlic extract is 1:2.

[0107] Thirdly, exemplarily, the reaction process of modified hydroxypropyl methylcellulose combined with dandelion extract is as follows:

[0108] Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred at 40°C for 1 hour. Then, activated dandelion extract (dandelion extract dissolved in 20% ethanol aqueous solution, and garlic extract concentration controlled at 40 mg / mL) was added. After reacting at pH 7 and 35°C for 2 hours, the modified hydroxypropyl methylcellulose-dandelion extract was obtained by freeze drying.

[0109] The mass ratio of modified hydroxypropyl methylcellulose to distilled water is 12:100, and the molar ratio of modified hydroxypropyl methylcellulose to dandelion extract is 1:2.

[0110] The present invention provides a process for preparing a plant-derived bactericidal and disinfecting granular composition, comprising the following steps:

[0111] Fatty acids, oleic acid, and isomeric tridecyl alcohol polyoxyethylene ether TO8 were mixed at 60°C for 20 minutes to obtain the first phase.

[0112] Deionized water, alkyl glycoside APG, and disodium cocoyl glutamate were mixed at 50°C for 10 min to obtain the second phase; the amount of deionized water used was twice the mass of alkyl glycoside APG08.

[0113] The chelating agent GL47, citric acid, protease P300, modified hydroxypropyl methylcellulose-plant extract, first phase and second phase were stirred at 55℃ for 30 min to obtain a mixed slurry.

[0114] The mixed slurry was granulated using a swing granulator, with the extrusion pressure controlled at 0.5 MPa, and then dried and cured to obtain a granular composition derived from plant sterilization and disinfection.

[0115] Comparative Example 1

[0116] The difference between Comparative Example 1 and Example 1 is that: unmodified plant extracts (i.e., oregano leaf extract, garlic extract, and dandelion extract) were used as raw materials to prepare a plant-derived bactericidal and disinfecting granular composition;

[0117] The plant-derived bactericidal and disinfectant granular compositions of Examples 1-3 and Comparative Example 1 were tested for antibacterial activity, solubility, and photostability. The antibacterial test followed GB / T 20944.3-2008. The solubility test conditions were: 37°C pure water, 50 rpm rotation speed, 280 nm detection wavelength, and dissolution rate test for 30 minutes, referring to the "Determination of Dissolution and Release Rate" in General Chapter 0931 of the Chinese Pharmacopoeia 2020. The photostability test involved UV-B irradiation for 500 hours, and the decrease in antibacterial rate was calculated. Specific test results are as follows:

[0118]

[0119] As can be seen from the performance test data table above, the plant-derived bactericidal and disinfectant granular composition prepared by the present invention has better antibacterial properties, water solubility, and UV resistance compared to plant-derived bactericidal and disinfectant granular compositions prepared using unmodified plant extracts as raw materials.

[0120] The foregoing has provided a detailed description of one embodiment of the present invention, but this description is merely a preferred embodiment and should not be construed as limiting the scope of the invention. All equivalent variations and modifications made within the scope of the claims of this invention should still fall within the patent coverage of this invention.

Claims

1. A preparation process for a plant-derived bactericidal and disinfectant granular composition, characterized in that, Includes the following steps: Fatty acids, oleic acid, and isomeric tridecyl alcohol polyoxyethylene ether TO8 were mixed at 60°C for 20 minutes to obtain the first phase. Deionized water, alkyl glycoside APG, and disodium cocoyl glutamate were mixed at 50°C for 10 min to obtain the second phase; the amount of deionized water used was twice the mass of alkyl glycoside APG08. The chelating agent GL47, citric acid, protease P300, modified hydroxypropyl methylcellulose-plant extract, first phase and second phase were stirred at 55℃ for 30 min to obtain a mixed slurry. The mixed slurry is granulated to obtain a granular composition derived from plant-based sterilization and disinfection.

2. The preparation process of the plant-derived bactericidal and disinfectant granular composition according to claim 1, characterized in that, A method for preparing modified hydroxypropyl methylcellulose-plant extract, comprising the following steps: Step 1: Under nitrogen protection, methyl methacrylate is mixed with ethyl 2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]acrylate, and azobisisobutyronitrile is added simultaneously. The mixture is reacted at a temperature of 65-80℃ to obtain UV-resistant modified methyl methacrylate. 2-Ethyl isocyanate methacrylate was added to UV-resistant modified methyl methacrylate and reacted at 75°C to obtain end-group activated UV-resistant modified methyl methacrylate. Step 2: Dissolve hydroxypropyl methylcellulose in anhydrous DMF, then add end-group activated UV-resistant modified methyl methacrylate, and react at 70-75℃ for 3 hours to obtain the modified hydroxypropyl methylcellulose carrier. Step 3: Modified hydroxypropyl methylcellulose is combined with oregano leaf extract, garlic extract and dandelion extract respectively through intermolecular hydrogen bonding to obtain modified hydroxypropyl methylcellulose-plant extract.

3. The preparation process of the plant-derived bactericidal and disinfectant granular composition according to claim 2, characterized in that, The molar ratio of methyl methacrylate to ethyl 2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]acrylate was controlled to be 5:

1.

4. The preparation process of the plant-derived bactericidal and disinfectant granular composition according to claim 2, characterized in that, The mass ratio of hydroxypropyl methylcellulose to anhydrous DMF is 10-15:100-150.

5. The preparation process of a plant-derived bactericidal and disinfecting granular composition according to claim 2, characterized in that, The molar ratio of hydroxypropyl methylcellulose to end-group activated UV-resistant modified methyl methacrylate is 3.5-4:

1.

6. The preparation process of a plant-derived bactericidal and disinfecting granular composition according to claim 2, characterized in that, The reaction process of modified hydroxypropyl methylcellulose combined with oregano leaf extract is as follows: Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred, then oregano leaf extract was added. The mixture was reacted at pH 5.0-6.5 and temperature 25-35℃ to obtain modified hydroxypropyl methylcellulose-oregano leaf extract.

7. The preparation process of a plant-derived bactericidal and disinfecting granular composition according to claim 2, characterized in that, The reaction process of modified hydroxypropyl methylcellulose combined with garlic extract is as follows: Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred, then activated garlic extract was added, and the mixture was reacted at pH 6-7 and temperature 25-35℃ to obtain modified hydroxypropyl methylcellulose-garlic extract.

8. The preparation process of a plant-derived bactericidal and disinfecting granular composition according to claim 2, characterized in that, The reaction process of modified hydroxypropyl methylcellulose combined with dandelion extract is as follows: Under nitrogen protection, modified hydroxypropyl methylcellulose was added to distilled water and stirred, followed by the addition of activated dandelion extract. The mixture was reacted at pH 6-7 and temperature 25-35℃ to obtain modified hydroxypropyl methylcellulose-dandelion extract.

9. A granular composition derived from plant-based sterilization and disinfection, characterized in that, The particulate composition is prepared by the method according to any one of claims 1-8, and the particulate composition comprises the following raw materials in parts by weight: 2-3 parts chelating agent GL47, 8-10 parts fatty acids, 5-6 parts oleic acid, 12-15 parts isomeric tridecyl polyoxyethylene ether TO8, 10-12 parts alkyl glycoside APG, 6-8 parts disodium cocoyl glutamate, 1.5-2 parts citric acid, 0.5-1 part protease P300, 2-3 parts hepta-enzyme complex, 3.5-4.7 parts modified hydroxypropyl methylcellulose-plant extract.

10. The granular composition for plant-derived sterilization and disinfection according to claim 9, characterized in that, The modified hydroxypropyl methylcellulose-plant extract comprises the following raw materials in parts by weight: 1.5-2 parts modified hydroxypropyl methylcellulose-oregano leaf extract, 1-1.5 parts modified hydroxypropyl methylcellulose-garlic extract, and 1-1.2 parts modified hydroxypropyl methylcellulose-dandelion extract.

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