Anti-wrinkle composition containing recombinant collagen as well as preparation method and application of anti-wrinkle composition

Abstract

The invention discloses an anti-wrinkle composition containing recombinant collagen as well as a preparation method and application of the anti-wrinkle composition, and belongs to the technical field of cosmetics and skin care products. The composition is prepared from the following active ingredients in percentage by mass: 0.01 percent to 20 percent of recombinant humanized I type collagen, 0.01 percent to 20 percent of recombinant humanized III type collagen, 0.01 percent to 10 percent of recombinant humanized XVII type collagen and 0.01 percent to 20 percent of space breeding desert algae extract. The three kinds of recombinant humanized collagen and the space breeding desert algae extract form a compound system, and when the total mass ratio of the three kinds of recombinant humanized collagen and the space breeding desert algae extract is within a specific range of 1.3: 1-4: 1, the three kinds of recombinant humanized collagen and the space breeding desert algae extract can achieve synergistic interaction to improve the skin barrier function. Based on the synergistic mechanism of the compound system, the composition disclosed by the invention can effectively promote and enhance the skin moisturizing and anti-wrinkle capability and repair the skin barrier, and is suitable for the development and application of anti-wrinkle, tightening and skin aging delaying skin care products.

Description

Technical Field

[0001] This invention relates to the field of cosmetics and skincare technology, and more specifically, to an anti-wrinkle composition containing recombinant collagen, its preparation method, and its application. Background Technology

[0002] With rising living standards and increased awareness of skincare, anti-wrinkle, firming, and anti-aging skincare products have become a significant development direction in the cosmetics market. The formation of wrinkles is primarily related to the reduction of collagen in the dermis, structural degeneration, and weakened basement membrane function. Simultaneously, impaired skin barrier function and moisture loss accelerate skin aging. Therefore, modern anti-wrinkle skincare products need to comprehensively enhance collagen regeneration, repair the skin barrier, and strengthen moisturizing capabilities to achieve a more comprehensive anti-aging effect.

[0003] Current anti-wrinkle products mostly use ingredients such as retinol, peptides, hyaluronic acid, and natural extracts, which work by promoting collagen production or improving hydration. However, these ingredients still have shortcomings in terms of safety, penetration, and efficacy. On the one hand, some ingredients may irritate or cause sensitization to the skin, especially limiting their effectiveness for sensitive skin. On the other hand, due to their molecular structure or physicochemical properties, these ingredients often have difficulty effectively penetrating the dermis, thus affecting their anti-wrinkle effect. In addition, the mechanisms of action of single ingredients are limited, making it difficult to meet multiple needs such as anti-wrinkle, firming, moisturizing, and anti-oxidation.

[0004] With the development of bioengineering technology, recombinant humanized collagen has become a research hotspot in the field of anti-wrinkle skincare due to its high homology with human collagen structure and good biocompatibility. Recombinant humanized type I, III, and XVII collagen can not only be effectively absorbed by the skin, but also play roles in replenishing collagen, rebuilding the dermal reticular structure, and repairing the basement membrane, significantly improving the skin's structural stability and elasticity. However, relying solely on recombinant collagen is still insufficient to achieve a multi-dimensional synergistic anti-aging effect.

[0005] In view of this, the present invention is proposed. Summary of the Invention

[0006] The purpose of this invention is to provide an anti-wrinkle composition containing recombinant collagen, its preparation method, and its application. This anti-wrinkle composition promotes collagen production, enhances the skin's moisturizing ability, and repairs the skin barrier through the synergistic effect of recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract. This significantly improves the skin's elasticity, firmness, and smoothness, effectively combats skin aging, and overcomes the traditional anti-aging product's challenge of balancing transdermal absorption, efficacy, and safety, thus fundamentally solving the problem of skin aging.

[0007] This invention is implemented as follows: In a first aspect, the present invention provides an anti-wrinkle composition containing recombinant collagen, comprising the following components by mass percentage: 0.01%~20% recombinant humanized type I collagen, 0.01%~20% recombinant humanized type III collagen, 0.01%~10% recombinant humanized type XVII collagen, and 0.01%~20% space-bred desert algae extract, wherein the recombinant humanized type I collagen, the recombinant humanized type III collagen, and the recombinant humanized type XVII collagen constitute recombinant humanized collagen, and the mass ratio of the recombinant humanized collagen to the space-bred desert algae extract is 1.3-4:1.

[0008] In a second aspect, the present invention provides a method for preparing an anti-wrinkle composition containing recombinant collagen according to any of the foregoing embodiments, comprising the following steps: S1. Weigh out each component according to the formula; S2. Dissolve the aqueous phase component at 80℃~90℃ and stir until homogenized and free of particles; S3. After cooling to below 45°C, add the remaining components and stir evenly to prepare an anti-wrinkle composition containing recombinant collagen.

[0009] Thirdly, the present invention provides the use of an anti-wrinkle composition containing recombinant collagen according to any one of the foregoing embodiments in the preparation of cosmetics.

[0010] The present invention has the following beneficial effects: This invention achieves multiple synergistic effects through a composite combination of recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and extracts from space-bred desert algae. This combination promotes collagen production, enhances skin's moisturizing ability, and repairs the skin barrier, thereby significantly improving skin elasticity, firmness, and smoothness. The product of this invention has multiple effects, including skin barrier repair, anti-aging care, collagen protection, collagen production promotion, and water retention, making it suitable for the research and application of anti-wrinkle, firming, and anti-aging skincare products. Detailed Implementation

[0011] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0012] This invention provides an anti-wrinkle composition containing recombinant collagen, comprising the following components by weight percentage: 0.01%~20% recombinant humanized type I collagen, 0.01%~20% recombinant humanized type III collagen, 0.01%~10% recombinant humanized type XVII collagen, and 0.01%~20% space-bred desert algae extract. The recombinant humanized type I collagen, recombinant humanized type III collagen, and recombinant humanized type XVII collagen constitute recombinant humanized collagen, and the mass ratio of recombinant humanized collagen to space-bred desert algae extract is 1.3-4:1.

[0013] This invention utilizes the synergistic effect of recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract to promote collagen production, enhance skin's moisturizing ability, and repair the skin barrier. This significantly improves skin elasticity, firmness, and smoothness, effectively combats skin aging, and overcomes the traditional anti-aging product's challenge of balancing transdermal absorption, efficacy, and safety, thus fundamentally solving the problem of skin aging.

[0014] Among them, the extract of space-bred desert algae, as a novel active ingredient, is rich in polysaccharides, peptides, and antioxidants. It possesses excellent moisturizing capabilities, significantly promotes the proliferation of skin fibroblasts, and improves skin firmness. Simultaneously, this extract also has soothing and antioxidant effects, mitigating damage to the skin from free radicals and external stimuli. Therefore, developing an anti-wrinkle composition that synergistically combines multiple recombinant collagen proteins with the space-bred desert algae extract offers enhanced safety, stronger penetration, and more comprehensive anti-aging efficacy, meeting the urgent needs of current technological development.

[0015] The extract of space-bred desert algae is CODE2.0 of the space-bred algae. Hassallia sp. CODE2.0 (also known as Hassallia The extract of sp. P06), the CODE2.0 of the algae. Hassallia The accession number for sp. CODE2.0 is CCTCC NO: M 20242054, the accession date is September 23, 2024, the depositary institution is the China Center for Type Culture Collection (CCTCC), and the deposit address is Wuhan University, Wuhan, China. The aforementioned *Hassaela* CODE2.0 ( Hassallia sp. CODE2.0) is a self-developed raw material of Danzi Group Co., Ltd.

[0016] Furthermore, it includes the following components by weight percentage: 0.05%~20% recombinant humanized type I collagen, 0.05%~20% recombinant humanized type III collagen, 0.05%~10% recombinant humanized type XVII collagen, and 0.05%~20% space-bred desert algae extract.

[0017] Furthermore, it includes the following components by weight percentage: 0.2%~20% recombinant humanized type I collagen, 0.5%~20% recombinant humanized type III collagen, 0.1%~10% recombinant humanized type XVII collagen, and 0.2%~20% space-bred desert algae extract.

[0018] The anti-wrinkle composition containing recombinant collagen provided by this invention is prepared by compounding recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract, and has the following effects: (1) Synergy between structural support and elastic repair: Recombinant humanized type I collagen: As the main structural protein of the dermis, it can directly replenish lost collagen fibers, build a "skeleton" support structure, and improve skin laxity and wrinkles (such as nasolabial folds and forehead wrinkles) caused by collagen loss. Recombinant humanized type III collagen: Primarily found in infant skin, it is responsible for maintaining skin elasticity and smoothness. Together with type I collagen, it forms a "mesh-like interwoven structure," enhancing skin's extensibility and resilience, and reducing the formation of dynamic wrinkles (such as expression lines).

[0019] (2) Synergistic effect of skin barrier repair and anti-aging: Recombinant humanized type XVII collagen: A transmembrane collagen, mainly distributed at the junction of the epidermis and dermis (basement membrane zone). It strengthens intercellular connections, promotes epidermal renewal and repair, and reduces moisture loss and external stimuli invasion caused by barrier damage. Space-bred desert algae extract: Rich in polysaccharides, it forms a moisturizing film to lock in skin moisture. Simultaneously, its antioxidant components (such as flavonoids and vitamin E) can scavenge free radicals, reducing oxidative damage to the skin from UV rays and pollution, indirectly protecting collagen fibers from degradation.

[0020] (3) Synergistic effect in promoting endogenous collagen production Recombinant humanized type I, III, and XVII collagen: As an "exogenous collagen template," it can be absorbed through the skin or bind to receptors on the surface of fibroblasts, activating collagen synthesis signaling pathways (such as the TGF-β pathway) and inducing the skin to synthesize more endogenous collagen, achieving a dual effect of "external supplementation and internal promotion." Space-bred desert algae extract: Its antioxidant components can improve the skin's inflammatory environment, creating favorable conditions for collagen production. Synergistic effect: The combination of direct exogenous collagen supplementation and endogenous collagen induction forms a virtuous cycle of "supplementation-activation-regeneration," fundamentally increasing skin collagen content and delaying the deepening of wrinkles. (4) Synergistic effect of moisturizing and anti-wrinkle metabolism Space-bred desert algae extract: In addition to polysaccharide moisturizing, its natural moisturizing factors (such as glycerin and amino acids) can regulate skin hydration, maintain the moisture content of the stratum corneum, and prevent fine lines (such as dry lines) caused by dryness. Recombinant humanized collagen (type I / III): Collagen fibers themselves are hygroscopic and can combine with water molecules to form a "hydrating gel," enhancing the skin's water-locking ability. Together with the desert algae extract, it maintains the skin's moisture state and reduces the causes of wrinkles caused by dryness. Synergistic effect: Moisturizing and collagen structure support promote each other—sufficient moisture can maintain the flexibility of collagen fibers, while the integrity of the collagen network can prevent rapid moisture loss, forming a "moisturizing-anti-wrinkle" metabolic closed loop.

[0021] In optional embodiments, the anti-wrinkle composition containing recombinant collagen further includes one or more of the following: emulsifiers, antioxidants, emollients, thickeners, moisturizers, chelating agents, skin conditioning agents, fragrances, preservatives, and pH adjusters, with the balance being water. Additionally, other functional ingredients may be included as needed.

[0022] In an optional embodiment, based on 100% of the total mass of the anti-wrinkle composition containing recombinant collagen, the content of emulsifier is 0-20%, the content of antioxidant is 0-10%, the content of emollient is 0-50%, the content of thickener is 0.01%-8%, the content of moisturizer is 0.01%-40%, the content of chelating agent is 0.01%-1%, the content of skin conditioning agent is 0-50%, the content of fragrance is 0-1%, the content of preservative is 0.01-3%, and the content of pH adjuster is 0-3%.

[0023] More specifically, based on 100% of the total mass of the anti-wrinkle composition containing recombinant collagen, the content of emulsifier is 0-10%, the content of antioxidant is 0-5%, the content of emollient is 0-20%, the content of thickener is 0.01%-1%, the content of moisturizer is 0.01%-20%, the content of chelating agent is 0.01%-0.08%, the content of skin conditioning agent is 0-20%, the content of fragrance is 0-0.2%, the content of preservative is 0.01%-2%, and the content of pH adjuster is 0-2%.

[0024] Furthermore, the emulsifier includes, but is not limited to, one or more of PEG-100 stearate, polyglycerol-3-methyl glucoside distearate, cetyl phosphate potassium, cetearyl glucoside, cetearyl olive oil ester, sodium methylstearoyl taurate, sodium methylstearoyl taurate, hydrogenated lecithin, sorbitan olive oil ester, and polyglycerol-3-distearate; it should be noted that, as an emulsifier, any emulsifier that can form a water-in-oil (W / O) or oil-in-water (O / W) emulsion anti-wrinkle cosmetic composition is acceptable, without particular limitation.

[0025] In some embodiments, the antioxidant includes, but is not limited to, one or more of vitamin E and its derivatives, vitamin C and its derivatives, ubiquinone and fullerene; preferably, the antioxidant is selected from vitamin E and its derivatives.

[0026] In some embodiments, the emollient includes, but is not limited to, triglycerides (ethylhexanoate), caprylic / capric triglycerides, pentaerythritol tetra(ethylhexanoate), butyl octyl salicylate, diethoxyethyl succinate, isododecane, rice germ oil, hydrogenated palm kernel oil, cocoa seed butter cetyl ethylhexanoate, isopropyl myristate, isopropyl palmitate, diisopropyl sebacate, polydimethylsiloxane, cetearyl alcohol, dibutyl adipate, C12-15 benzoates, dioctyl carbonate, cocoyl octyl / capric ester, polydimethylsiloxane, octyl polymethylsiloxane, cyclopentamethoxysiloxane, and phenyltrimethylpolysiloxane. It should be noted that other emollients commonly used in the cosmetics field can be used as emollients, without particular limitation. Preferably, the emollient is selected from one or more of C12-15 benzoates, polydimethylsiloxane, and rice germ oil.

[0027] In some embodiments, the thickener, as an aqueous phase component, includes, but is not limited to, xanthan gum, acrylate / C10-30 alkanol acrylate crosspolymers, carbomer, ammonium acryloyldimethyl taurate / VP copolymer, and sodium acrylate / sodium acryloyldimethyl taurate copolymer; preferably, the thickener is selected from, or more of, acrylate / C10-30 alkanol acrylate crosspolymers, ammonium acryloyldimethyl taurate / VP copolymer, and sodium acrylate / sodium acryloyldimethyl taurate copolymer.

[0028] In some embodiments, the moisturizer includes, but is not limited to, one or more of glycerin, propylene glycol, seaweed extract, sodium hyaluronate, aloe vera extract, jojoba oil, polyethylene glycol / polypropylene glycol-14 / 7 dimethyl ether, xylitol, and sorbitol; as the aqueous phase component, any moisturizer commonly used in the cosmetics industry is acceptable, without particular limitations. Preferably, the moisturizer is selected from glycerin.

[0029] In some embodiments, the chelating agent includes, but is not limited to, one or a combination of two or more of disodium EDTA, tetrasodium EDTA, and sodium phytate.

[0030] In some embodiments, the skin conditioning agent includes, but is not limited to, one or more of the following: vitamin E, scutellaria baicalensis extract, bisabolol, scutellaria baicalensis root extract, sea buckthorn fruit extract, glucan, and vitamin B5, which have the effect of repairing damaged skin.

[0031] In some embodiments, the fragrance agent includes, but is not limited to, one or two of fragrances and essential oils.

[0032] In some embodiments, as an aqueous phase component, the preservative includes, but is not limited to, one or more of phenoxyethanol, ethylhexylglycerin, hexanediol, and pentanediol.

[0033] In some embodiments, as an aqueous phase component, the pH adjuster includes, but is not limited to, one or more of arginine, sodium hydroxide, potassium hydroxide, triethanolamine, and aminomethylpropanol.

[0034] Furthermore, the present invention provides a method for preparing the above-mentioned anti-wrinkle composition containing recombinant collagen, comprising the following steps: S1. Weigh out each component according to the formula; S2. Dissolve the aqueous phase component at 80℃~90℃ and stir until homogenized and free of particles; S3. After cooling to below 45°C, add the remaining components and stir evenly to prepare an anti-wrinkle composition containing recombinant collagen.

[0035] The preparation method provided by this invention is simple, easy to operate, and suitable for mass production.

[0036] Furthermore, the present invention also provides the application of the above-mentioned anti-wrinkle composition containing recombinant collagen in the preparation of cosmetics.

[0037] In an optional implementation, the cosmetic is any one of skin care water, essence, lotion, face cream, face mask, sunscreen, BB cream and makeup base.

[0038] The features and performance of the present invention will be further described in detail below with reference to embodiments.

[0039] I. Application Examples Example 1 This embodiment provides an anti-wrinkle composition containing recombinant collagen, comprising the following components by weight percentage: Phase B: 0.05% recombinant humanized type I collagen, 0.05% recombinant humanized type III collagen, 0.05% recombinant humanized type XVII collagen, 0.05% space-bred desert algae extract, and 0.5% preservatives; Phase A: 0.05% chelating agent, 0.5% thickener, 5% humectant, and the remainder deionized water.

[0040] The chelating agent is EDTA-2Na, the thickener is sodium acrylate / sodium acryloyl dimethyl taurate copolymer, the humectant is glycerin, and the preservative is phenoxyethanol.

[0041] The specific preparation method is as follows: S1. Weigh out each component according to the formula; S2. Dissolve phase A at 85℃ and stir until homogenized and free of particles; S3. After cooling to below 45 ℃, add phase B and stir evenly to prepare the anti-wrinkle composition.

[0042] Examples 2-6 Examples 2-6 and Comparative Examples 1-2 provide an anti-wrinkle composition containing recombinant collagen. The difference from Example 1 is that the specific amounts of the components are different, while the others remain unchanged. The specific changes in the amounts of each component are shown in Table 1 below.

[0043] Table 1. Formulation details of Examples 1-6 and Comparative Examples 1-2

[0044] II. Application of Comparative Examples Based on Example 3, multiple comparative examples were set up around four important components: recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract. These examples were set up by removing one or more components or replacing one component. These examples are Comparative Examples 3 to 16.

[0045] Among them, Comparative Examples 3 to 6 are selected from one of the four components: recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract, and one of them is omitted.

[0046] Comparative Examples 7 to 10 consist of two of the following four components: recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract, which were omitted from the comparison examples.

[0047] Comparative Example 11 did not include any of the following four components: recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract.

[0048] Comparative Examples 12 to 15 involved replacing four components—recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract—with conventional raw materials. Specifically, Comparative Example 12 used conventional animal-derived type I collagen to replace recombinant humanized type I collagen, Comparative Example 13 used conventional animal-derived type III collagen to replace recombinant humanized type III collagen, Comparative Example 14 used conventional animal-derived type XVII collagen to replace recombinant humanized type XVII collagen, and Comparative Example 15 used conventional trehalose to replace space-bred desert algae extract.

[0049] The amount of key components used in Comparative Example 16 exceeds the limits defined in this invention.

[0050] III. Experimental Testing Experiment 1: Elastase Activity Inhibition Test Elastase activity inhibition assays are significant in anti-aging research because they directly assess the ability of skincare products to inhibit elastin degradation. Elastin is a key component in maintaining skin elasticity and firmness. Although it constitutes a small proportion of the dermis, it plays a crucial role in maintaining skin elasticity and preventing wrinkles and sagging. However, with age, elastin is gradually broken down by elastase, leading to loss of skin elasticity and the appearance of wrinkles, sagging, and other signs of aging. Through elastase activity inhibition assays, researchers can evaluate the inhibitory effect of active ingredients in skincare products on elastase activity. This test can reveal whether a skincare composition effectively prevents or slows down elastin degradation, thus reflecting the product's potential to maintain skin elasticity and delay aging. Inhibiting elastase activity is an important mechanism for delaying wrinkle formation and maintaining skin firmness, and therefore has key evaluative value in anti-aging research.

[0051] Therefore, an elastase activity inhibition test was used, and the specific test procedure is as follows: The compositions prepared in Examples 1-6 and Comparative Examples 1-16 were diluted with deionized water to a concentration of 5% to prepare sample solutions, which were then prepared for later use. 1 mL of the sample solution and 1 mL of elastase solution were added to a 5 mL centrifuge tube, thoroughly mixed, and reacted at 37°C for 5 min. Then, 1 mL of substrate solution was added, and the reaction was continued at 37°C for 30 min. After incubation, the mixture was centrifuged at 1000 rpm for 5 min, and 200 μL of the supernatant was transferred to a 96-well plate. The absorbance was measured at 405 nm using a microplate reader. A blank substrate group (using an equal volume of 10% DMSO instead of the substrate), a 100% enzyme activity control group, and a blank control group were also set up. Each group was performed in triplicate, with sodium selenolex as a positive control. The formula for calculating the elastase inhibition rate is as follows: Elastase inhibition rate = [1 - (Y1 - Y2) / (Y3 - Y4)] x 100% In the formula: Y1 is the absorbance value of the test sample + elastase solution + substrate solution, Y2 is the absorbance value of the test sample + elastase solution + 10% DMSO, Y3 is the absorbance value of the PBS buffer solution + elastase solution + substrate solution, and Y4 is the absorbance value of the PBS buffer solution + elastase solution + 10% DMSO. The results of the elastase activity inhibition test are shown in Table 2 below.

[0052] Table 2 Results of elastase activity inhibition test

[0053] Table 2 shows that the anti-wrinkle compositions of Examples 1-6 all exhibited excellent elastase inhibitory activity, with the highest clearance rate reaching 76.32%.

[0054] Comparing the data from Experimental Examples 1-6 and Comparative Examples 1-16, we can see that: The overall performance of the example groups was significantly better than that of the comparative groups. The key indicators were as follows: the elastase inhibition rates of Examples 1-6 were significantly higher than those of all comparative groups. Specifically, Comparative Examples 12-15 replaced a single component in the composition of this invention with a functionally similar ingredient, resulting in a significant decrease in their inhibition rate compared to the example groups, directly demonstrating the irreplaceable nature of the synergistic effect of the four components of this invention. Comparative Example 16 also showed a significant decrease in inhibition rate compared to the example groups because the amount of the key component exceeded the limits specified in this invention.

[0055] Regarding the relationship between concentration and effect, studies have shown that in a four-component coexisting system, the elastase inhibition rate and anti-wrinkle effect exhibit a dose-dependent characteristic—within the concentration range defined in this invention, the higher the concentration, the higher the elastase inhibition rate, and the anti-wrinkle effect is enhanced simultaneously; however, when the concentration exceeds this range (e.g., Comparative Example 16), as the concentration further increases, the elastase inhibition rate decreases, and the anti-wrinkle effect is reduced.

[0056] Comparative Examples 3-15 show that the absence or replacement of a single ingredient leads to a decrease in efficacy: if any ingredient is missing, the inhibition rate decreases by more than 50% compared to the complete formula, and the anti-wrinkle effect is significantly weakened. Among them, the space-bred desert algae extract is an important support for improving the effect; its absence or replacement with ordinary algae extract significantly reduces the inhibition rate; the "space breeding" process is the key to its high activity. After replacing recombinant humanized collagen with ordinary collagen and space algae extract with algae extract, the inhibition rate decreased by 42.7% and 38.5% respectively, confirming that the recombinant humanized structure and the unique active ingredients of space algae are the core of efficacy; when a single component is used alone, the elastase inhibition rate is less than 20%, and the anti-wrinkle effect is weak. At the same time, recombinant humanized collagen and space-bred desert algae extract have a synergistic effect; only when the ratio of the two is 1.3-4:1, the inhibition rate is maintained at a high level of 35.24%-76.32%; when the ratio is outside this range (e.g., comparative examples 1 and 2), the inhibition effect becomes significantly worse.

[0057] Conclusion: Recombinant humanized type I, III, and XVII collagen and space-bred desert algae extract form a complete anti-wrinkle system through complementary components and synergistic functions. The absence or replacement of any component will destroy the synergistic effect, and the optimal synergistic effect can only be achieved when the ratio of the two is within the range of 1.3-4:1. This verifies that the coexistence of the four components and their specific ratio are the key mechanisms for achieving efficient anti-wrinkle effects.

[0058] Experiment 2: Analysis of transepidermal water loss rate of skin Transepidermal water loss (TEWL), measured by a Tewameter TM300, is an important parameter reflecting skin moisture loss and barrier function. A lower measured value indicates better skin barrier function. The detection method for transepidermal water loss analysis is as follows: Subject selection criteria: healthy female subjects aged 20 to 50 years; and subjects with positive skin after a 10% lactic acid stinging test (determined to be sensitive skin); slight facial redness; 110 subjects were selected, and the compositions obtained in Examples 1 to 6 and Comparative Examples 1 to 16 were used as samples for use evaluation, with 5 subjects randomly selected from each sample.

[0059] Application area: Face Usage frequency: Use once in the morning and once in the evening for 28 consecutive days, with each use being 1.2g.

[0060] Test environment conditions: temperature 21±1℃, relative humidity 50±5%.

[0061] Experimental design: single-blind, randomized, self-controlled before-and-after, face (selected on the opposite side of the area where lactic acid solution was applied, according to a randomization table).

[0062] Evaluation method details: Skin transepidermal water loss rate analysis, the results are shown in Table 3 below.

[0063] Table 3. Data on the rate of change in transepidermal water loss from facial skin

[0064] As shown in Table 3, the experimental results indicate that there is a strict synergistic effect range between the total amount of recombinant humanized type I, III, and XVII collagen and the amount of space-bred desert algae extract. When the two are combined in a mass ratio of 1.3:1 to 4:1, the composition shows a significantly better effect than other ratios in reducing transepidermal water loss (TEWL) (TEWL reduction is greater than 20%). This ratio is the critical range for triggering the synergistic effect; outside this range, the efficacy of the composition is significantly reduced, and may even produce adverse effects (as shown in Comparative Examples 1 and 2). This proves that this ratio relationship is an indispensable technical feature of the present invention.

[0065] Regarding the necessity of the active ingredient combination: Comparative examples (Comparative Examples 3-11) demonstrating the elimination of single or multiple components confirm that recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract together constitute the necessary technical features to achieve the aforementioned superior efficacy. The absence of any one or more of these components, or any combination of two components, will prevent the achievement of the TEWL reduction effect of this invention. This indicates that the four active ingredients constitute a functionally interdependent and inseparable synergistic system, and their technical effect is not a simple summation of the functions of each component.

[0066] Regarding the irreplaceability of specific raw material sources: Comparative examples (Comparative Examples 12-15) demonstrating that the specific source of the active ingredient is key to achieving the stated technical effect were confirmed. Replacing recombinant humanized collagen with conventional animal-derived collagen, or replacing space-bred desert algae extract with ordinary algae extract, resulted in the efficacy of the composition reverting to ordinary levels, significantly different from the technical solution of this invention. This result proves that the unique properties imparted by the specific sources, such as "recombinant humanized" and "space-bred," are the basis for the synergistic effect, an effect that cannot be achieved with conventional raw materials. The concentration of the component in Comparative Example 16 exceeded the range of this invention; with further increases in concentration, the effect of reducing transepidermal water loss decreased.

[0067] In summary, the core of this invention lies in the discovery and verification of a compound system composed of recombinant humanized type I, III, and XVII collagen from specific sources and extracts from space-bred desert algae. This system only produces a surprising synergistic effect in improving skin barrier function when the total mass ratio of the active ingredients is within a specific range of 1.3:1 to 4:1. The combination of active ingredients, the specific ratio range, and the source of raw materials in this invention are interconnected and work together to form a complete, non-obvious, yet highly effective technical whole, exhibiting significant substantive features and substantial progress compared to existing technologies.

[0068] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. An anti-wrinkle composition containing recombinant collagen, characterized in that, The product comprises the following components by weight percentage: 0.01%~20% recombinant humanized type I collagen, 0.01%~20% recombinant humanized type III collagen, 0.01%~10% recombinant humanized type XVII collagen, and 0.01%~20% space-bred desert algae extract. The recombinant humanized type I collagen, recombinant humanized type III collagen, and recombinant humanized type XVII collagen constitute recombinant humanized collagen. The mass ratio of the recombinant humanized collagen to the space-bred desert algae extract is 1.3-4:

1.

2. The anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that, It includes the following components by weight percentage: 0.05%~20% recombinant humanized type I collagen, 0.05%~20% recombinant humanized type III collagen, 0.05%~10% recombinant humanized type XVII collagen, and 0.05%~20% space-bred desert algae extract.

3. The anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that, It includes the following components by weight percentage: 0.2%~20% recombinant humanized type I collagen, 0.5%~20% recombinant humanized type III collagen, 0.1%~10% recombinant humanized type XVII collagen, and 0.2%~20% space-bred desert algae extract.

4. The anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that, The anti-wrinkle composition containing recombinant collagen further includes one or more of the following: emulsifier, antioxidant, emollient, thickener, moisturizer, chelating agent, skin conditioning agent, fragrance, preservative, and pH adjuster, with the balance being water.

5. The anti-wrinkle composition containing recombinant collagen according to claim 4, characterized in that, Based on 100% of the total mass of the anti-wrinkle composition containing recombinant collagen, the content of the emulsifier is 0-20%, the content of the antioxidant is 0-10%, the content of the emollient is 0-50%, the content of the thickener is 0.01%-8%, the content of the moisturizer is 0.01%-40%, the content of the chelating agent is 0.01%-1%, the content of the skin conditioning agent is 0-50%, the content of the fragrance agent is 0-1%, the content of the preservative is 0.01%-3%, and the content of the pH adjuster is 0-3%.

6. The anti-wrinkle composition containing recombinant collagen according to claim 4, characterized in that, Based on 100% of the total mass of the anti-wrinkle composition containing recombinant collagen, the content of the emulsifier is 0-10%, the content of the antioxidant is 0-5%, the content of the emollient is 0-20%, the content of the thickener is 0.01%-1%, the content of the moisturizer is 0.01%-20%, the content of the chelating agent is 0.01%-0.08%, the content of the skin conditioning agent is 0-20%, the content of the fragrance agent is 0-0.2%, the content of the preservative is 0.01-2%, and the content of the pH adjuster is 0-2%.

7. The anti-wrinkle composition containing recombinant collagen according to any one of claims 4 to 6, characterized in that, The emulsifier is selected from one or more of PEG-100 stearate, polyglycerol-3-methyl glucoside distearate, cetyl phosphate potassium, cetearyl glucoside, cetearyl olive oil ester, sodium methylstearoyl taurate, sodium methylstearoyl taurate, hydrogenated lecithin, sorbitan olive oil ester and polyglycerol-3-distearate; And / or, the antioxidant is selected from one or more of vitamin E and its derivatives, vitamin C and its derivatives, ubiquinone and fullerene; And / or, the emollient is selected from one or more of the following: triglycerides (ethylhexanoate), caprylic / capric triglycerides, pentaerythritol tetra(ethylhexanoate), butyl octyl salicylate, diethoxyethyl succinate, isododecane, rice germ oil, hydrogenated palm kernel oil, cocoa seed butter cetyl ethylhexanoate, isopropyl myristate, isopropyl palmitate, diisopropyl sebacate, polydimethylsiloxane, cetearyl alcohol, dibutyl adipate, C12-15 alcohol benzoate, dioctyl carbonate, cocoyl alcohol-caprylate / capric acid ester, polydimethylsiloxane, octyl polymethylsiloxane, cyclopentamethoxysiloxane, and phenyltrimethyl polysiloxane; And / or, the thickener is selected from one or more of xanthan gum, acrylate / C10~30 alkanol acrylate crosspolymers, carbomer, ammonium acryloyldimethyl taurate / VP copolymer, and sodium acrylate / sodium acryloyldimethyl taurate copolymer; And / or, the moisturizer is selected from one or more of glycerin, propylene glycol, seaweed extract, sodium hyaluronate, aloe vera extract, jojoba oil, polyethylene glycol / polypropylene glycol-14 / 7 dimethyl ether, xylitol, and sorbitol; And / or, the chelating agent is one or a combination of two or more of disodium EDTA, tetrasodium EDTA, and sodium phytate; And / or, the skin conditioning agent is selected from one or more of vitamin E, scutellaria baicalensis extract, bisabolol, scutellaria baicalensis root extract, prickly pear fruit extract, glucan, and vitamin B5; And / or, the fragrance agent includes one or two of fragrances and essential oils; And / or, the preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, hexanediol, and pentanediol; And / or, the pH adjuster is selected from one or more of arginine, sodium hydroxide, potassium hydroxide, triethanolamine, and aminomethylpropanol.

8. A method for preparing an anti-wrinkle composition containing recombinant collagen according to any one of claims 1 to 7, characterized in that, Includes the following steps: S1. Weigh out each component according to the formula; S2. Dissolve the aqueous phase component at 80℃~90℃ and stir until homogenized and free of particles; S3. After cooling to below 45°C, add the remaining components and stir evenly to prepare an anti-wrinkle composition containing recombinant collagen.

9. The use of the anti-wrinkle composition containing recombinant collagen according to any one of claims 1 to 7 in the preparation of cosmetics.

10. The application according to claim 9, characterized in that, The cosmetic product is any one of the following: skin care water, essence, lotion, face cream, face mask, sunscreen, BB cream, and makeup base.

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