Cell culture method

Abstract

The invention discloses a cell culture method, and relates to the technical field of cell culture.The cell culture system comprises a box body, a stirring assembly used for stirring cells and a culture solution is arranged in the box body, a collecting assembly used for assisting observation is arranged in the box body, and a solution changing assembly used for water injection is further arranged in the box body; a matching plate rotates to drive a long swing rod to swing, the long swing rod swings to drive a large gear to rotate around a small gear, a culture solution in the stirring box can be intermittently added into the stirring box through a liquid inlet pipe, a transmission shaft rotates to drive stirring blades to move, and the culture solution and cells in the stirring box can be stirred; the first cam drives the sweeping rod to do telescopic motion in the fixed rod through the pulley, bubbles generated by stirring and dead cells in the culture process can be discharged from the interior of the stirring box through the sweeping rod while the cells and the culture solution are stirred, and the dead cells can be effectively prevented from influencing the cell culture speed.

Description

Technical Field

[0001] This invention relates to the field of cell culture technology, specifically cell culture methods. Background Technology

[0002] Cell culture is a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce, and maintain their main structures and functions. Cell culture is also called cell cloning technology. The formal term in biology is cell culture technology. Cell culture is an indispensable process for both the whole of bioengineering technology and one of its components, biological cloning technology.

[0003] Chinese Patent (Publication No.: CN112342122A) relates to the field of bacterial culture technology and discloses a contamination-resistant bacterial culture system and method for a laboratory. The contamination-resistant bacterial culture system includes a box, culture dishes, and a placement platform. A vertical plate is bolted to the inner wall of one side of the box, a horizontal plate is fixed to one side of the vertical plate, and an empty box is fixed to one side of the horizontal plate. One end of a straight tube is connected to the bottom of the empty box, and the other end of the straight tube extends into the culture dish. Multiple stirring rods are fixed to the outside of the straight tube. A placement groove is provided on the top of the placement platform. This invention is rationally designed. By rotating the culture dish and simultaneously shaking it up and down, and through the cooperation of the stirring rods, the culture medium is stirred, thus creating circulation in the culture medium. This suspends the bacteria, ensures adequate light exposure, and allows oxygen to be introduced into the culture dish to supplement the oxygen required for aerobic bacterial growth, thereby ensuring the effectiveness of bacterial culture.

[0004] While the aforementioned patent can stir the culture medium to create circulation, suspend the cells, and ensure adequate light exposure, it does not address the issue of dead cells during cultivation. This requires manual cleaning, which can negatively impact the growth of viable cells. Furthermore, prolonged accumulation of cells in the culture vessel reduces growth rate and increases the cultivation cycle. Therefore, a new cell culture method is proposed. Summary of the Invention

[0005] The purpose of this invention is to provide a cell culture method to solve the problems mentioned in the background above.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a cell culture method, wherein the cell culture method is implemented through a cell culture system, and the cell culture method includes the following steps: Step 1, Adding Materials: Add the cleaning solution, culture medium, and cells to be cultured to the designated location; Step 2: Mixing and stirring: Thoroughly mix the culture medium and cells; Step 3, Infusion: The culture medium is intermittently injected into the interior of the mixing tank from different directions; Step 4: Cleaning: Remove dead cells and air bubbles generated during mixing from inside the mixing tank; Step 5: Collection: Collect the impurities and other materials after cleaning.

[0007] Preferably, the cell culture system includes a box, the inside of which is provided with a stirring assembly for stirring cells and culture medium, a collection box slidably connected inside the box, an observation port on the side of the box, a collection assembly for auxiliary observation inside the box, a medium replacement assembly for water filling inside the box, and an observation microscope fixedly connected inside the box.

[0008] Preferably, the stirring assembly includes a motor, a long chute, and a long swing arm. The stirring assembly controls the collecting assembly to perform the collecting operation via the long swing arm, and controls the liquid exchange assembly to perform the filling operation via the long chute. A mating plate is fixedly connected to the output end of the motor. The mating plate is rotatably connected to the long swing arm via a pin. A transmission shaft is rotatably connected to the long swing arm via a pin. A first deflector plate is fixedly connected to the surface of the long swing arm. Several sets of stirring blades and small gears are fixedly connected to the surface of the transmission shaft. The small gears mesh with a large gear.

[0009] Preferably, a turntable is fixedly connected to the upper end of the drive shaft, a long slide groove is provided above the turntable, a first fixed plate is fixedly connected to the side of the long slide groove, a first slide rod is slidably connected to the inside of the first fixed plate through an arc groove, a first cam is fixedly connected to the surface of the drive shaft, a sweeping rod is slidably connected to the surface of the first cam, a fixed rod is fixedly connected to the surface of the drive shaft, the sweeping rod slides inside the fixed rod, a fixed sleeve is slidably connected to the surface of the sweeping rod, the fixed sleeve is fixedly connected to the mixing tank, and a second cam is fixedly connected to the surface of the drive shaft.

[0010] Preferably, the fluid replacement assembly includes a second fixed plate, which is fixedly connected to a long slide groove. A second slide rod is slidably connected inside the second fixed plate. A fluid storage tank is slidably connected below the second slide rod. An inlet pipe is fixedly connected to the side of the fluid storage tank. A limit sleeve is provided on the surface of the inlet pipe, and a pressure rod is provided on the surface of the limit sleeve. A moving plate is fixedly connected to the side of the second slide rod. An actuating disc is provided inside the moving plate. A fan blade is provided on one side of the actuating disc, and a bellows is provided around the fan blade. The bellows is fixedly connected to the fluid storage tank. A base is connected to the bottom of the fluid storage tank by several sets of springs.

[0011] Preferably, the collection assembly includes a first swing rod, which is rotatably connected to a long swing rod via a pin. A telescopic rod is provided on the side of the first swing rod, and one end of the telescopic rod is connected to a U-shaped groove via a hinge ball. A sliding plate is slidably connected inside the U-shaped groove, and a second deflector is fixedly connected to the upper end of the sliding plate. A spring is provided inside the second deflector. A garbage bin is detachably connected to the side of the first swing rod, and a discharge plate is provided directly above the garbage bin. The discharge plate is movably connected to the mixing tank via a pin.

[0012] Preferably, an L-shaped rod is fixedly connected to the lower part of the feeding plate, and a wiping rod is hinged to the lower end of the L-shaped rod via a hinge ball joint. The wiping rod is located on the surface of the observation mirror. A moving rod is hinged to the lower part of the feeding plate via a hinge ball joint. A connecting rod is rotatably connected to the lower part of the moving rod via a pin. A hinged tube is rotatably connected to the surface of the connecting rod via a pin. A fixed block is hinged to the hinged tube via a hinge ball joint. The fixed block is detachably connected to the trash can. A brush is provided on the surface of the wiping rod. A push rod is fixedly connected to the surface of the moving rod. The first sliding rod is hinged to the feeding plate via a hinge ball joint.

[0013] Preferably, the push rod has a liquid outlet pipe on its side, and a cleaning box is movably connected above the liquid outlet pipe. The cleaning box is fixedly connected to the box body. A support rod is movably connected below the feeding plate. One end of the support rod is movably connected to the mixing box. The motor is fixedly connected to the box body. Several sets of corrugated blocks are fixedly connected to the lower end face of the mixing box. The mixing box is fixedly connected to the box body. The collection box is located on the side of the storage tank. Three sets of stirring blades are arranged at a 120-degree angle along the axis of the transmission shaft. Four sets of springs are arranged at the four corners between the base and the storage tank.

[0014] Preferably, the U-shaped groove and the mixing tank are slidably connected, a reset element is provided below the U-shaped groove, the telescopic rod and the first swing rod are slidably connected, a reset element is provided inside the telescopic rod, a reset element is also provided at the contact position between the limiting sleeve and the liquid inlet pipe, the base and the mixing tank are fixedly connected, the first fixing plate and the second fixing plate are symmetrically arranged along the turntable axis, and a feeding port is provided above the mixing tank.

[0015] Preferably, the second cam, in conjunction with the telescopic rod, enables the slide plate to move forward and backward while simultaneously moving left and right. When the stirring assembly stirs the cells and culture medium, the height of the water and cells inside the stirring tank is always consistent with the height of the feed plate. The airflow generated by the fan blades in the liquid exchange assembly cleans impurities while also cleaning impurities adhering to the surface of the sweeping rod. The movement of the waste bin and the feed plate is achieved through relative movement via a fixed block.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. This invention utilizes an electric motor to rotate a mating plate, which in turn causes a long pendulum to swing. The swinging of the pendulum causes a large gear to rotate around a small gear, which in turn causes a transmission shaft to rotate. Simultaneously, the transmission shaft drives a turntable to rotate. The turntable, via a lever on its surface, moves a long sliding groove left and right, which in turn moves a second fixed plate. The second fixed plate, via an internal groove, causes a second sliding rod to slide inside a storage tank, allowing the culture medium inside to be intermittently added to the mixing tank through an inlet pipe. The rotation of the transmission shaft causes the stirring blades to move, thus stirring the culture medium and cells inside the mixing tank. Simultaneously, the rotation of the transmission shaft causes a first cam to rotate, which, via a pulley, drives a sweeping rod to extend and retract inside the fixed rod. While stirring the cells and culture medium, the sweeping rod removes air bubbles generated during stirring and dead cells from the mixing tank, effectively preventing dead cells from affecting the efficiency of cell culture.

[0017] 2. In this invention, the second fixed plate drives the second sliding rod to move up and down, and the second sliding rod drives the moving plate to move up and down. The moving plate drives the actuating plate to rotate through the protrusions inside, and the actuating plate drives the fan blades to rotate. The wind force generated by the rotating fan blades can gather the air bubbles and dead cells on the surface to one place, so that they can be scraped away when the sweeping rod moves. At the same time, when the sweeping rod rotates, it will press down the pressure rod. The pressure rod presses down the liquid inlet pipe through the limiting sleeve. At this time, the culture medium injected into the mixing tank by the liquid inlet pipe changes from spraying upward to spraying downward, which can effectively reduce the air bubbles generated inside the mixing tank and improve the mixing between the cells and the culture medium.

[0018] 3. This invention uses a long swing arm to drive the first lever plate. The swinging of the first lever plate contacts the wave block at the bottom of the mixing tank, causing the mixing tank to shake. Simultaneously, the swinging of the long swing arm drives the first swing rod, which in turn drives the telescopic rod. At the same time, the transmission shaft drives the second cam to rotate, which in turn drives the sliding plate. The telescopic rod also drives the sliding plate. The movement of the sliding plate drives the second lever plate to move inside the mixing tank, which can push the cells that cannot be stirred and settled inside the mixing tank, so that the cells can fully contact the culture medium. At the same time, the movement of the long slide groove drives the first fixed plate to move. The first fixed plate drives the first slide rod to move up and down through the internal slide groove. The first slide rod drives the discharge plate to move, which can more fully discharge the scraped cells and improve work efficiency.

[0019] 4. In this invention, the movement of the feeding plate drives the L-shaped rod to move up and down. The movement of the L-shaped rod can wipe the mirror surface of the observation mirror. At the same time, the movement of the feeding plate drives the movement of the moving rod, which drives the push rod to push the liquid outlet tube to move. The movement of the liquid outlet tube can evenly spray the cleaning liquid inside the cleaning box onto the mirror surface of the observation mirror for wiping. Simultaneously, the movement of the moving rod drives the connecting rod to swing. The connecting rod drives the hinge tube to swing through the pin. The hinge tube drives the fixed block to shake through the hinge ball. The fixed block drives the waste bin to shake. The shaking of the waste bin can increase the number of cells collected. At the same time, the dead cells and air bubbles scraped off by the rotation of the sweeping rod are intermittently discharged into the waste bin by the intermittent swing of the feeding plate for easy collection. Attached Figure Description

[0020] Figure 1 This is a flowchart of the cell culture method of the present invention; Figure 2 This is a schematic diagram of the external structure of the present invention; Figure 3 This is a schematic diagram of the structure of the present invention with part of the box removed; Figure 4 This is a schematic diagram of the internal structure of the present invention; Figure 5 This is a schematic diagram showing the internal structure of the mixing tank of the present invention without the mixing tank. Figure 6 This is a schematic diagram of the stirring assembly structure of the present invention; Figure 7 This is a partial structural diagram of the stirring assembly of the present invention; Figure 8 This is a schematic diagram of a partial component structure of the fluid exchange assembly of the present invention; Figure 9 This is a schematic diagram of the component structure for the present invention; Figure 10 This is a schematic diagram of a partial structure of the components collected in this invention; Figure 11 This invention collects schematic diagrams of the internal structure of some parts of the component; Figure 12 This is a schematic diagram of a partial component structure of the present invention; In the diagram: 1. Box body; 2. Observation port; 3. Collection box; 4. Mixing box; 5. Observation mirror; 6. Waste bin; 100. Mixing assembly; 101. Motor; 102. Matching plate; 103. Long swing rod; 104. First lever; 105. Small gear; 106. Large gear; 107. Drive shaft; 108. Mixing blade; 109. Turntable; 110. Long slide; 111. First fixing plate; 112. First slide rod; 113. First cam; 114. Fixing sleeve; 115. Fixing rod; 116. Sweeping rod; 117. Second cam; 118. Wave block; 200. Liquid changing assembly; 201. Pressure rod; 20 2. Limiting sleeve; 203. Inlet pipe; 204. Storage tank; 205. Base; 206. Second slide bar; 207. Second fixing plate; 208. Air box; 209. Fan blade; 210. Actuating disc; 211. Moving plate; 300. Collection assembly; 301. First swing rod; 302. Telescopic rod; 303. Slide plate; 304. U-shaped groove; 305. Second actuating plate; 306. Discharge plate; 307. L-shaped rod; 308. Wiping rod; 309. Cleaning box; 310. Outlet pipe; 311. Support rod; 312. Push rod; 313. Connecting rod; 314. Hinge pipe; 315. Fixing block; 316. Moving rod. Detailed Implementation

[0021] The purpose of this invention is to solve the problems of existing bacterial culture systems, which typically rely on manual stirring and shaking of the culture dish, resulting in low bacterial culture efficiency, poor culture results, and the inability to clean up dead bacteria.

[0022] Please see Figures 1 to 12 This invention provides a technical solution: a cell culture method, which is implemented through a cell culture system and includes the following steps: Step 1, Adding Materials: Add the cleaning solution, culture medium, and cells to be cultured to the designated location; Step 2: Mixing and stirring: Thoroughly mix the culture medium and cells; Step 3, Infusion: The culture medium is intermittently injected into the interior of the mixing tank 4 from different directions; Step 4, Cleaning: Remove dead cells and air bubbles generated during mixing from inside mixing tank 4; Step 5: Collection: Collect the impurities and other materials after cleaning.

[0023] like Figure 3As shown, the cell culture system includes a box 1, inside which is a stirring assembly 100 for stirring cells and culture medium, a collection box 3 is slidably connected inside the box 1, an observation port 2 is opened on the side of the box 1, inside which is a collection assembly 300 for auxiliary observation, a medium replacement assembly 200 for water filling is also provided inside the box 1, and an observation mirror 5 is fixedly connected inside the box 1.

[0024] like Figure 6 As shown, the stirring assembly 100 includes a motor 101, a long chute 110, and a long swing rod 103. The stirring assembly 100 controls the collection assembly 300 to perform collection work through the long swing rod 103, and controls the liquid exchange assembly 200 to perform priming work through the long chute 110. A mating plate 102 is fixedly connected to the output end of the motor 101. The mating plate 102 is rotatably connected to the long swing rod 103 through a pin. The long swing rod 103 is rotatably connected to a transmission shaft 107 through a pin. A first lever plate 104 is fixedly connected to the surface of the long swing rod 103. Several sets of stirring blades 108 and a small gear 105 are fixedly connected to the surface of the transmission shaft 107. The small gear 105 is meshed with a large gear 106.

[0025] like Figure 7 As shown, a turntable 109 is fixedly connected to the upper end of the drive shaft 107. A long slide groove 110 is provided above the turntable 109. A first fixed plate 111 is fixedly connected to the side of the long slide groove 110. A first slide rod 112 is slidably connected to the inside of the first fixed plate 111 through an arc groove. A first cam 113 is fixedly connected to the surface of the drive shaft 107. A sweeping rod 116 is slidably connected to the surface of the first cam 113. A fixed rod 115 is fixedly connected to the surface of the drive shaft 107. The sweeping rod 116 slides inside the fixed rod 115. A fixed sleeve 114 is slidably connected to the surface of the sweeping rod 116. The fixed sleeve 114 is fixedly connected to the mixing tank 4. A second cam 117 is fixedly connected to the surface of the drive shaft 107.

[0026] like Figure 8 As shown, the fluid replacement assembly 200 includes a second fixed plate 207, which is fixedly connected to the long slide groove 110. A second slide rod 206 is slidably connected inside the second fixed plate 207. A storage tank 204 is slidably connected below the second slide rod 206. An inlet pipe 203 is fixedly connected to the side of the storage tank 204. A limit sleeve 202 is provided on the surface of the inlet pipe 203. A pressure rod 201 is provided on the surface of the limit sleeve 202. A moving plate 211 is fixedly connected to the side of the second slide rod 206. An actuating disc 210 is provided inside the moving plate 211. A fan blade 209 is provided on one side of the actuating disc 210. A bellows 208 is provided around the fan blade 209. The bellows 208 is fixedly connected to the storage tank 204. A base 205 is connected to the bottom of the storage tank 204 through several sets of springs.

[0027] like Figure 9 As shown, the collection component 300 includes a first swing rod 301, which is rotatably connected to a long swing rod 103 via a pin. A telescopic rod 302 is provided on the side of the first swing rod 301. One end of the telescopic rod 302 is connected to a U-shaped groove 304 via a hinge ball. A slide plate 303 is slidably connected inside the U-shaped groove 304. A second deflector plate 305 is fixedly connected to the upper end of the slide plate 303. A spring is provided inside the second deflector plate 305. A garbage bin 6 is detachably connected to the side of the first swing rod 301. A discharge plate 306 is provided directly above the garbage bin 6. The discharge plate 306 is movably connected to the mixing tank 4 via a pin.

[0028] like Figure 10 As shown, an L-shaped rod 307 is fixedly connected to the lower part of the feeding plate 306. The lower end of the L-shaped rod 307 is hinged to a wiping rod 308 via a hinge ball joint. The wiping rod 308 is located on the surface of the observation mirror 5. A moving rod 316 is hinged to the lower part of the feeding plate 306 via a hinge ball joint. A connecting rod 313 is rotatably connected to the lower part of the moving rod 316 via a pin. A hinge tube 314 is rotatably connected to the surface of the connecting rod 313 via a pin joint. A fixing block 315 is hinged to the hinge tube 314 via a hinge ball joint. The fixing block 315 is detachably connected to the garbage bin 6. A brush is provided on the surface of the wiping rod 308. A push rod 312 is fixedly connected to the surface of the moving rod 316. The first sliding rod 112 is hinged to the feeding plate 306 via a hinge ball joint.

[0029] like Figure 12 As shown, a liquid outlet pipe 310 is provided on the side of the push rod 312, and a cleaning box 309 is movably connected above the liquid outlet pipe 310. The cleaning box 309 is fixedly connected to the box body 1. A support rod 311 is movably connected below the discharge plate 306. One end of the support rod 311 is movably connected to the mixing box 4. The motor 101 is fixedly connected to the box body 1. Several sets of corrugated blocks 118 are fixedly connected to the lower end face of the mixing box 4. The mixing box 4 is fixedly connected to the box body 1. The collection box 3 is located on the side of the liquid storage tank 204. Three sets of stirring blades 108 are arranged at a 120-degree angle along the axis of the transmission shaft 107. Four sets of springs are arranged at the four corners between the base 205 and the liquid storage tank 204.

[0030] Furthermore, the U-shaped groove 304 is slidably connected to the mixing tank 4, and a reset element is provided below the U-shaped groove 304. The telescopic rod 302 is slidably connected to the first swing rod 301, and a reset element is provided inside the telescopic rod 302. A reset element is also provided at the contact position between the limiting sleeve 202 and the liquid inlet pipe 203. The base 205 is fixedly connected to the mixing tank 4. The first fixed plate 111 and the second fixed plate 207 are symmetrically arranged along the axis of the turntable 109. A feeding port is provided above the mixing tank 4.

[0031] Furthermore, the second cam 117, in conjunction with the telescopic rod 302, enables the slide plate 303 to move forward and backward while simultaneously moving left and right. When the stirring assembly 100 stirs the cells and culture medium, the height of the water and cells inside the stirring tank 4 is always consistent with the height of the feed plate 306. The wind generated by the fan blades 209 in the liquid exchange assembly 200 cleans impurities while also cleaning the impurities adhering to the surface of the sweeping rod 116. The movement of the waste bin 6 and the feed plate 306 is achieved through relative movement via the fixing block 315.

[0032] Working principle: After installation, the culture medium is first added to the storage tank 204, and the cleaning solution is added to the cleaning tank 309. The cells to be cultured are then placed inside the stirring tank 4. The motor 101 is then started, driving the mating plate 102 to rotate. The mating plate 102, via a pin, drives the long swing rod 103 to swing. The long swing rod 103, via a pin, drives the large gear 106 to rotate around the small gear 105. The small gear 105 drives the transmission shaft 107 to rotate, and the transmission shaft 107 drives the stirring blade 10... 8 rotates, and at the same time, the drive shaft 107 drives the turntable 109 to rotate. The turntable 109 drives the long slide groove 110 to move left and right through the lever. The long slide groove 110 drives the second fixed plate 207 to move. The second fixed plate 207 drives the second slide rod 206 to slide inside the storage tank 204 through the internal slide groove. It can intermittently spray the culture medium inside the storage tank 204 into the inside of the mixing tank 4 through the liquid inlet pipe 203. The rotation of the stirring blade 108 stirs the cells and culture medium, which can improve the efficiency of cell culture. At this time, the drive shaft 107 drives the first cam 113 to rotate and the fixed rod 115 to rotate. The rotation of the first cam 113 intermittently pushes the sweeping rod 116 to slide inside the fixed rod 115, which can scrape the bubbles generated by the stirring blade 108 and the dead cells to the discharge plate 306 and discharge them into the mixing tank 4. When the sweeping rod 116 is at the closest position between the first cam 113 and the inner surface of the fixed sleeve 114, the first fixed plate 111 drives the first sliding rod 112 to move. The first sliding rod 112 drives the discharge plate 306 to be level with the discharge port of the mixing tank 4. At this time, the movement of the sweeping rod 116 can scrape the bubbles and dead cells to the surface of the discharge plate 306 and discharge them. When the sweeping rod 116 continues to move, it pushes the pressure rod 201 to move downward. The pressure rod 201 pushes the liquid inlet pipe 203 downward through the limiting sleeve 202. By changing the position of the culture medium sprayed from the inside of the storage tank 204 to the inside of the mixing tank 4, the generation of bubbles during cell culture can be effectively reduced, and the efficiency of the sweeping rod 116 in processing bubbles and dead cells can be improved. When the second slide bar 206 moves up and down, it drives the motion plate 211 to move up and down. The motion plate 211 drives the dial 210 to rotate in both directions. The rotation of the dial 210 drives the fan blade 209 to rotate. The wind generated by the rotation of the fan blade 209 can blow the air bubbles and dead cells suspended on the surface of the mixing tank 4 to one side, so that the sweeping bar 116 can scrape them out of the mixing tank 4. At the same time, the wind generated by the rotation of the fan blade 209 can blow off the impurities on the surface of the sweeping bar 116, and the sweeping bar 116 will scrape the impurities out of the system again. At this time, the long swing arm 103 swings, causing the first lever 104 to swing. The first lever 104 can push the wave block 118 to vibrate. The vibration of the wave block 118 causes the mixing tank 4 to vibrate, which can vibrate the culture medium and cells being stirred inside the mixing tank 4. This can fully suspend air bubbles and dead cells on the surface of the culture medium, improving the culture efficiency. At the same time, the long swing arm 103 drives the first swing rod 301 to swing through the pin. The first swing rod 301 pushes the telescopic rod 302 to move. The telescopic rod 302 drives the slide plate 303 to slide inside the U-shaped groove 304 through the hinge ball. At the same time, the second cam 117 pushes the slide plate 303 to slide inside the U-shaped groove 304, which can make the U-shaped groove 304 move back and forth while moving left and right. The movement of the slide plate 303 drives the second lever 305 to move inside the mixing tank 4. The movement of the second lever 305 can suspend the cells that have settled inside the mixing tank 4, improve the contact between the cells and the culture medium, and improve the culture efficiency. When the scraped-off impurities fall through the feed plate 306, the first swing rod 301 swings, causing the garbage bin 6 to move and collect the dead cells that fall from the feed plate 306. At the same time, the swing of the feed plate 306 causes the L-shaped rod 307 to move. The L-shaped rod 307 moves through the hinge ball, causing the wiping rod 308 to swing. The swing of the wiping rod 308 can wipe the surface of the observation mirror 5. At the same time, the movement of the feed plate 306 causes the moving rod 316 to move downward. The moving rod 316 causes the connecting rod 313 to rotate around the pin. The connecting rod 313 drives the hinge tube 314 to move through the pin. The hinge tube 314 drives the fixed block 315 to move through the hinge ball. The movement of the fixed block 315 causes the garbage bin 6 to shake, which can settle the impurities inside the garbage bin 6. While the wiping rod 308 cleans the surface of the observation mirror 5, the movement of the moving rod 316 drives the push rod 312 to move and push the liquid outlet pipe 310 to swing. At the same time, the cleaning solution inside the cleaning box 309 is sprayed onto the surface of the observation mirror 5 through the liquid outlet pipe 310. The swinging of the liquid outlet pipe 310 can increase the area of ​​the cleaning solution sprayed on the surface of the observation mirror 5, which can improve the ability of external personnel to observe the progress of cell culture inside the stirring box 4 at any time.

Claims

1. A cell culture method, wherein the cell culture method is implemented through a cell culture system, characterized in that: The cell culture method includes the following steps: Step 1, Adding Materials: Add the cleaning solution, culture medium, and cells to be cultured to the designated location; Step 2: Mixing and stirring: Thoroughly mix the culture medium and cells; Step 3, Infusion: The culture medium is intermittently injected into the interior of the mixing tank (4) from different directions; Step 4, Cleaning: Remove dead cells and air bubbles generated by stirring from the inside of the mixing tank (4); Step 5: Collection: Collect the impurities and other materials after cleaning.

2. The cell culture method according to claim 1, characterized in that: The cell culture system includes a box (1), inside which is a stirring assembly (100) for stirring cells and culture medium. Inside the box (1) is a collection box (3) slidably connected. On the side of the box (1) is an observation port (2). Inside the box (1) is a collection assembly (300) for auxiliary observation. Inside the box (1) is a liquid exchange assembly (200) for water injection. Inside the box (1) is an observation mirror (5).

3. The cell culture method according to claim 2, characterized in that: The stirring assembly (100) includes a motor (101), a long chute (110), and a long swing rod (103). The stirring assembly (100) controls the collection assembly (300) to perform collection work through the long swing rod (103). The stirring assembly (100) controls the liquid exchange assembly (200) to perform immersion work through the long chute (110). The output end of the motor (101) is fixedly connected to a mating plate (102). The mating plate (102) is rotatably connected to the long swing rod (103) through a pin. The long swing rod (103) is rotatably connected to a transmission shaft (107) through a pin. A first lever plate (104) is fixedly connected to the surface of the long swing rod (103). Several sets of stirring blades (108) and small gears (105) are fixedly connected to the surface of the transmission shaft (107). The small gears (105) are meshed with a large gear (106).

4. The cell culture method according to claim 3, characterized in that: The upper end of the drive shaft (107) is fixedly connected to a turntable (109). A long slide groove (110) is provided above the turntable (109). A first fixed plate (111) is fixedly connected to the side of the long slide groove (110). A first slide rod (112) is slidably connected inside the first fixed plate (111) through an arc groove. A first cam (113) is fixedly connected to the surface of the drive shaft (107). A sweeping rod (116) is slidably connected to the surface of the first cam (113). A fixed rod (115) is fixedly connected to the surface of the drive shaft (107). The sweeping rod (116) slides inside the fixed rod (115). A fixed sleeve (114) is slidably connected to the surface of the sweeping rod (116). The fixed sleeve (114) is fixedly connected to the mixing tank (4). A second cam (117) is fixedly connected to the surface of the drive shaft (107).

5. The cell culture method according to claim 4, characterized in that: The fluid exchange assembly (200) includes a second fixing plate (207), which is fixedly connected to the long slide groove (110). A second slide rod (206) is slidably connected inside the second fixing plate (207). A storage tank (204) is slidably connected below the second slide rod (206). An inlet pipe (203) is fixedly connected to the side of the storage tank (204). A limiting sleeve (202) is provided on the surface of the inlet pipe (203). The surface is provided with a pressure rod (201), and the second slide rod (206) is fixedly connected to a moving plate (211). The moving plate (211) is provided with a dial plate (210) inside. A fan blade (209) is provided on one side of the dial plate (210). A bellows (208) is provided around the fan blade (209). The bellows (208) is fixedly connected to the liquid storage tank (204). The liquid storage tank (204) is connected to a base (205) below by several sets of springs.

6. The cell culture method according to claim 5, characterized in that: The collection assembly (300) includes a first swing rod (301), which is rotatably connected to a long swing rod (103) via a pin. A telescopic rod (302) is provided on the side of the first swing rod (301). One end of the telescopic rod (302) is connected to a U-shaped groove (304) via a hinge ball. A sliding plate (303) is slidably connected inside the U-shaped groove (304). A second lever (305) is fixedly connected to the upper end of the sliding plate (303). A spring is provided inside the second lever (305). A garbage bin (6) is detachably connected to the side of the first swing rod (301). A feeding plate (306) is provided directly above the garbage bin (6). The feeding plate (306) is movably connected to the mixing tank (4) via a pin.

7. The cell culture method according to claim 6, characterized in that: An L-shaped rod (307) is fixedly connected to the lower part of the feed plate (306). The lower end of the L-shaped rod (307) is connected to a wiping rod (308) via a hinge ball joint. The wiping rod (308) is located on the surface of the observation mirror (5). A moving rod (316) is connected to the lower part of the feed plate (306) via a hinge ball joint. A connecting rod (313) is rotatably connected to the lower part of the moving rod (316) via a pin. A hinged tube (314) is rotatably connected to the surface of the connecting rod (313) via a pin joint. A fixing block (315) is connected to the hinged tube (314) via a hinge ball joint. The fixing block (315) is detachably connected to the trash can (6). A brush is provided on the surface of the wiping rod (308). A push rod (312) is fixedly connected to the surface of the moving rod (316). The first sliding rod (112) is connected to the feed plate (306) via a hinge ball joint.

8. The cell culture method according to claim 7, characterized in that: The push rod (312) is provided with a liquid outlet pipe (310) on its side. A cleaning box (309) is movably connected above the liquid outlet pipe (310). The cleaning box (309) is fixedly connected to the box body (1). A support rod (311) is movably connected below the feed plate (306). One end of the support rod (311) is movably connected to the mixing box (4). The motor (101) is fixedly connected to the box body (1). Several sets of wave blocks (118) are fixedly connected to the lower end face of the mixing box (4). The mixing box (4) is fixedly connected to the box body (1). The collection box (3) is located on the side of the storage tank (204). Three sets of stirring blades (108) are arranged at a 120-degree angle along the axis of the transmission shaft (107). Four sets of springs are arranged at the four corners between the base (205) and the storage tank (204).

9. The cell culture method according to claim 8, characterized in that: The U-shaped groove (304) is slidably connected to the mixing tank (4). A reset element is provided below the U-shaped groove (304). The telescopic rod (302) is slidably connected to the first swing rod (301). A reset element is provided inside the telescopic rod (302). A reset element is also provided at the contact position between the limiting sleeve (202) and the liquid inlet pipe (203). The base (205) is fixedly connected to the mixing tank (4). The first fixing plate (111) and the second fixing plate (207) are symmetrically arranged along the axis of the turntable (109). A feeding port is provided above the mixing tank (4).

10. The cell culture method according to claim 9, characterized in that: The second cam (117) works in conjunction with the telescopic rod (302) to enable the slide plate (303) to move back and forth while moving left and right. When the stirring assembly (100) stirs the cells and culture medium, the height of the water and cells inside the stirring box (4) is always consistent with the height of the feed plate (306). The wind generated by the fan blades (209) in the liquid exchange assembly (200) cleans the impurities and also cleans the impurities adhering to the surface of the sweeping rod (116). The movement of the garbage bin (6) and the feed plate (306) is achieved through the fixed block (315) to achieve relative movement.

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