A cell suction device for immune cell detection of neuroinfection

Abstract

The application discloses a cell suction device for immune cell detection of nerve infection, and relates to the technical field of cell suction devices.The cell suction device comprises a suction device, a cell filter is arranged at the front end of the suction device, and an expansion and contraction structure is fixedly connected in the inner cavity of the cell filter.The cell suction device has an expandable and contractible end head, can be quickly and easily installed and replaced with a suction nozzle, has high connection firmness with the suction nozzle, and has a clamping sleeve arranged on the periphery of the cell suction device and matched with the gripping curve of a hand, the clamping sleeve has an anti-skid pattern on the periphery, and the clamping sleeve can be connected with a wrist, so that the suction device is prevented from being separated from the hand and the stability of the cell suction device is improved.The suction end head has a replaceable filter screen structure, the filter screen is connected through magnetic attraction, and the filter screen can be selected as a mesh structure or a membrane structure, so that the functions of screening out oversized cells, adhering double cells and even dead cells are realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell suction device, and particularly relates to a cell suction device for immune cell detection of nerve infection. BACKGROUND

[0002] In the field of neuroscience research and clinical diagnosis, the detection and treatment of diseases related to nerve infection have always been an important research area. As an important part of the immune system of the body, immune cells play a key role in the process of nerve infection. The changes in the number, type and functional state of immune cells can reflect the severity and progression of nerve infection. Therefore, accurate and efficient detection of immune cells related to nerve infection is of great significance for early diagnosis, disease monitoring and treatment selection. At present, in the field of immune cell detection, cell suction device is an important tool for obtaining cell samples. However, the existing cell suction device has some shortcomings in the detection of nerve infection immune cells.

[0003] On the one hand, during the cell suction process, it is difficult to accurately control the type and quantity of suction cells, and impurities such as large cells and adherent double cells are easily mixed in, affecting the accuracy of subsequent detection results. These impurities may interfere with the normal analysis of immune cells, leading to misdiagnosis or missed diagnosis, and thus affecting the judgment and treatment of nerve infection diseases.

[0004] On the other hand, the existing cell suction device has defects in operation convenience and stability. During a long operation process, due to the smooth surface of the device or unreasonable design, the operator is prone to hand fatigue and hand slip, affecting the precision and efficiency of the suction operation. Moreover, because the suction end of the cell suction device is connected with the suction nozzle through interference, there is a risk of falling during the process of sucking cells, and both installation and replacement require force to pull out. Therefore, it is of great practical significance to develop a cell suction device that can accurately filter nerve infection immune cells, is convenient and stable to operate. SUMMARY

[0005] In view of the defects in the above background art, a technical solution of a cell suction device for immune cell detection of nerve infection is provided.

[0006] The cell suction device comprises a suction device, a cell filter is arranged at the front end of the suction device, an expansion and contraction structure is fixedly connected in the inner cavity of the cell filter, a suction nozzle is connected to the front end of the cell filter, and a silica gel anti-skid member is wrapped around the outer circle of the rear section of the suction device.

[0007] The suction device comprises a suction handle, a suction end fixed at the front end of the suction handle, and a control button arranged at the front section of the outer surface of the suction handle, and the outer side of the middle section of the suction handle is connected with a negative pressure suction pipe of a vacuum pump.

[0008] The silicone anti-slip component includes an anti-slip sleeve, a connector fixedly connected to the rear end face of the anti-slip sleeve, and a canvas strap fixedly connected to the rear end face of the connector, with a rubber restraint ring fitted on the outer surface of the canvas strap.

[0009] The cell filter includes a sleeve screwed onto the front end of the suction end, a front magnetic ring fixed in the rear section of the inner cavity of the sleeve, and a rear magnetic ring placed in the rear section of the inner cavity of the sleeve. A silicone ring is clamped between the rear magnetic ring and the front magnetic ring, and a filter membrane is fixed on the front side of the silicone ring.

[0010] The expansion and contraction structure includes several support rods fixedly connected to the front section of the inner wall of the sleeve, a micro servo motor fixed to one end of the support rods that converges with each other, and a lead screw connected to the output shaft of the micro servo motor via a coupling. A nut is movable on the thread of the rear section of the outer ring of the lead screw, and a bearing is rotatably provided at the front end of the outer ring of the lead screw. Several sets of X-shaped hinges are hinged to the outer ring of the bearing and the nut. A tile-shaped expansion piece is hinged to the outer end of each X-shaped hinge. The outer surface of the tile-shaped expansion piece contacts the inner wall of the suction nozzle.

[0011] In the above-mentioned technical solution of a cell aspiration device for detecting immune cells in nerve infections, preferably: the front end of the aspiration head is provided with an external threaded nozzle, which is used to connect the rear end of the sleeve to the aspiration head through the external threaded nozzle, and the outer ring surface of the aspiration handle is fixedly connected to the inner wall of the anti-slip sleeve by glue.

[0012] In the above-mentioned technical solution of a cell aspiration device for detecting immune cells in nerve infection, preferably: the outer surface of the anti-slip sleeve is provided with a concave surface that conforms to the grip curve of the human hand, and the outer side wall of the anti-slip sleeve is provided with an opening for the control button and the negative pressure tube to pass through.

[0013] In the above-mentioned technical solution of a cell aspiration device for detecting immune cells in nerve infection, preferably: all corners of the canvas strap are rounded and chamfered, and the inner surface of the canvas strap is wrapped and in contact with the human wrist. Multiple grooves are opened on the inner and outer wall surfaces of the rubber restraint ring to increase the friction with the outer surface of the canvas strap and to constrain the opening and closing size of the rear section of the canvas strap.

[0014] In the above-mentioned technical solution of a cell aspiration device for detecting immune cells in nerve infections, preferably: the front section of the outer ring of the cannula is inserted into the rear section region of the inner cavity of the aspiration nozzle, and the rear end port of the cannula is provided with a thread that matches the external threaded end of the aspiration tip.

[0015] Preferably, the front magnetic ring and the rear magnetic ring are magnetically attracted to each other, and the side walls of the front magnetic ring and the rear magnetic ring that are close to each other are in close contact with the front and rear surfaces of the silica gel ring.

[0016] Preferably, the filter membrane has a plurality of holes for filtering large cells and adhering double cells on the periphery.

[0017] Preferably, the middle and rear sections of the lead screw are provided with threads matched with the nut.

[0018] Preferably, the X-shaped hinges are arranged in a ring array with the middle axis of the lead screw as the base point, and each X-shaped hinge comprises two cross-connected connecting rods, and the front two ends of the X-shaped hinge are respectively hinged to the outer surface of the bearing and the inner front section of the tile-shaped expansion piece; the rear two ends of the X-shaped hinge are respectively hinged to the outer ring of the nut and slidingly connected to the inner rear section of the tile-shaped expansion piece.

[0019] Preferably, the inner rear section of the tile-shaped expansion piece is fixedly connected with a sliding groove, and the rear outer end of the X-shaped hinge is connected with a sliding block matched with the sliding groove.

[0020] The cell suction device for detecting immune cells for nerve infection provided by the above technical solution has the following advantages compared with the prior art:

[0021] The cell suction device for detecting immune cells for nerve infection provided by the above technical solution has the following advantages compared with the prior art: BRIEF DESCRIPTION OF DRAWINGS

[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments of the present invention or the prior art will be briefly introduced and explained below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 A schematic diagram of the overall structure of the cell extractor;

[0024] Figure 2 A schematic diagram of the main body of the suction device;

[0025] Figure 3 A diagram illustrating the anti-slip strap;

[0026] Figure 4 A schematic diagram of the suction head and suction nozzle;

[0027] Figure 5 A schematic diagram of the filter element for drawing cells from inside the end cap;

[0028] Figure 6 A schematic diagram of the expansion and contraction structure inside the end cap.

[0029] Appendix Figure 1 -Appendix Figure 6 The correspondence between the components is as follows:

[0030] 1. Suction device; 11. Suction handle; 12. Suction end; 2. Silicone anti-slip component; 21. Anti-slip sleeve; 22. Canvas strap; 23. Connector; 24. Rubber restraint ring; 3. Cell filter component; 31. Sleeve; 32. Filter membrane; 33. Silicone ring; 34. Rear magnetic ring; 35. Front magnetic ring; 4. Suction nozzle; 5. Expansion and contraction structure; 51. Support rod; 52. Miniature servo motor; 53. Lead screw; 54. Nut; 55. Bearing; 56. Tile-shaped expansion plate; 57. X-shaped hinge; 58. Slide groove. Detailed Implementation

[0031] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described below are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0032] To provide a clearer explanation and illustration of the technical solution and implementation of the present invention, the following describes preferred embodiments of the technical solution of the present invention.

[0033] Embodiment one: the embodiment is a kind of for nerve infection immune cell detection with cell suction device.The suction device 1 includes suction handle 11 and suction end head 12, suction handle 11 front end fixed suction end head 12, control button is arranged on the outer surface front section, and the outer side middle section is connected with the negative pressure suction pipe of vacuum pump.Silicone antiskid part 2, the inner cavity side wall of antiskid jacket 21 is fixedly connected with the outer circle surface of suction handle 11 by glue, antiskid jacket 21 rear end face fixed connector 23, connector 23 rear end head fixed canvas hanging belt 22, canvas hanging belt 22 outer surface is covered with rubber restraint ring 24.Cell filter 3 sleeve 31 is connected with suction end head 12 by the external thread end mouth of suction end head 12 front end and is screwed, sleeve 31 inner cavity middle rear section fixed front end magnetic ring 35, rear section places rear end magnetic ring 34, rear end magnetic ring 34 and front end magnetic ring 35 between clamping silica gel ring 33, silica gel ring 33 front side fixed filter membrane 32, filter membrane 32 has a plurality of for filtering large cell, adhesion double cell holes all around.Inflatable structure 5, several pieces of support rod 51 are fixedly connected with the inner cavity side wall front section of sleeve 31, support rod 51 are gathered to one end fixed micro servo motor 52, screw rod 53 is connected with the output shaft of micro servo motor 52 by coupling, screw rod 53 outer circle rear section screw thread moves up movable nut 54, outer circle front end rotationally arranged bearing 55, bearing 55 and movable nut 54 outer circle are articulated with several groups of X-shaped articulating pieces 57, X-shaped articulating pieces 57 outer side end head is respectively articulated tile-shaped expansion piece 56, tile-shaped expansion piece 56 outer surface and suction nozzle 4 inner cavity side wall contact.In actual use, suction nozzle 4 is clamped in cell filter 3 front end, operator holds suction handle 11, carries out negative pressure suction by control button control vacuum pump, makes cell from suction nozzle 4, when passing through cell filter 3, filter membrane 32 filters out oversized cell, adhesion double cell, completes cell suction.

[0034] In the initial state, the nut 54 is located at the outer rear section of the screw rod 53 close to the micro servo motor 52, at this time, the X-shaped hinge 57 is in the contracted state, driving the tile-shaped expansion piece 56 to gather inward, so that the inner cavity space of the suction nozzle 4 is relatively small. When it is necessary to adjust the inner cavity space of the suction nozzle 4 to adapt to different cell suction requirements, the micro servo motor 52 is started by the control button, the micro servo motor 52 drives the screw rod 53 to rotate, the nut 54 moves forward on the thread of the outer rear section of the screw rod 53, pushing the rear end of the X-shaped hinge 57 to move forward. Since the front two ends of the X-shaped hinge 57 are respectively hinged to the outer surface of the bearing 55 and the inner front section of the tile-shaped expansion piece 56, the rear two ends are respectively hinged to the outer circle of the nut 54 and slidingly connected to the inner rear section of the tile-shaped expansion piece 56, and the inner rear section of the tile-shaped expansion piece 56 is fixedly connected with the sliding groove 58, and the rear outer end of the X-shaped hinge 57 is connected with the sliding block matched with the sliding groove 58, the X-shaped hinge 57 is gradually unfolded, driving the tile-shaped expansion piece 56 to expand outward, thereby increasing the inner cavity space of the suction nozzle 4 to meet the requirements of different cell suction.

[0035] In the initial state, the nut 54 is located at the outer rear section of the screw rod 53 close to the micro servo motor 52, at this time, the X-shaped hinge 57 is in the contracted state, driving the tile-shaped expansion piece 56 to gather inward, so that the inner cavity space of the suction nozzle 4 is relatively small. When it is necessary to adjust the inner cavity space of the suction nozzle 4 to adapt to different cell suction requirements, the micro servo motor 52 is started by the control button, the micro servo motor 52 drives the screw rod 53 to rotate, the nut 54 moves forward on the thread of the outer rear section of the screw rod 53, pushing the rear end of the X-shaped hinge 57 to move forward. Since the front two ends of the X-shaped hinge 57 are respectively hinged to the outer surface of the bearing 55 and the inner front section of the tile-shaped expansion piece 56, the rear two ends are respectively hinged to the outer circle of the nut 54 and slidingly connected to the inner rear section of the tile-shaped expansion piece 56, and the inner rear section of the tile-shaped expansion piece 56 is fixedly connected with the sliding groove 58, and the rear outer end of the X-shaped hinge 57 is connected with the sliding block matched with the sliding groove 58, the X-shaped hinge 57 is gradually unfolded, driving the tile-shaped expansion piece 56 to expand outward, thereby increasing the inner cavity space of the suction nozzle 4 to meet the requirements of different cell suction.

[0036] In the fourth embodiment, the connection between the front magnetic ring 35 and the rear magnetic ring 34 and the replacement process of the filter membrane 32 in the cell filter 3 are described. The front magnetic ring 35 and the rear magnetic ring 34 are magnetically attracted to each other, and the side walls of the front magnetic ring 35 and the rear magnetic ring 34 close to each other are in close contact with the front and rear surfaces of the silica gel ring 33. This connection mode not only ensures the stability of the silica gel ring 33 and the filter membrane 32 in the sleeve 31, but also facilitates disassembly and replacement. When the filter membrane 32 needs to be replaced, the sleeve 31 is unscrewed from the suction tip 12. Due to the magnetic attraction between the front magnetic ring 35 and the rear magnetic ring 34, the rear magnetic ring 34 can be easily removed from the sleeve 31. Then, the silica gel ring 33 and the filter membrane 32 are removed, a new filter membrane 32 is installed, and the silica gel ring 33, the rear magnetic ring 34, and the front magnetic ring 35 are reinstalled. Finally, the sleeve 31 is re-screwed onto the suction tip 12, and the replacement of the filter membrane 32 is completed, ensuring the filtration of the cell suction device.

[0037] According to the above-mentioned preferred technical solution, the working process of the technical solution is described: the operator first adjusts the opening and closing size of the canvas hanging strap 22 in the silica gel anti-slip part 2 according to the actual use demand and his own operation habit, then puts the rubber restraint ring 24 on the canvas hanging strap 22, and uses the friction force generated between the inner and outer side walls of the rubber restraint ring 24 and the outer surface of the canvas hanging strap 22 to constrain the opening and closing size of the rear section of the canvas hanging strap 22, so that the suction device 1 is stably hung on the wrist, and the palm holds the suction handle 11. At this time, the outer surface of the anti-slip sleeve 21 fits the curved surface of the human hand holding curve to provide a comfortable holding experience, and the opening on the outer side wall of the anti-slip sleeve 21 does not affect the operation of the control button and the connection between the negative pressure pipe and the middle section of the outer side of the suction handle 11.

[0038] According to the situation of the cells to be sucked, if it is necessary to adjust the inner cavity space of the suction nozzle 4, the operator starts the micro servo motor 52 in the expansion and contraction structure 5 through the control button. The output shaft of the micro servo motor 52 drives the screw rod 53 to rotate. The screw thread set in the rear section of the screw rod 53 makes the nut 54 move on the screw thread in the rear section of the outer circle of the screw rod 53. Since the X-shaped articulated members 57 are arranged in a ring array with the central axis of the screw rod 53 as the base point, and each X-shaped articulated member 57 consists of two cross-connected connecting rods, the front two ends of the X-shaped articulated member 57 are respectively hinged to the outer surface of the bearing 55 and the inner front section of the tile-shaped expansion piece 56, and the rear two ends are respectively hinged to the outer circle of the nut 54 and slidably connected to the inner rear section of the tile-shaped expansion piece 56. The inner rear section of the tile-shaped expansion piece 56 is fixedly connected with a sliding groove 58, and the rear outer end of the X-shaped articulated member 57 is connected with a sliding block matched with the sliding groove 58. When the nut 54 moves, it pushes the rear end of the X-shaped articulated member 57 to move, so that the X-shaped articulated member 57 gradually expands or contracts, and in turn drives the tile-shaped expansion piece 56 to expand outward or gather inward to be connected with the suction nozzle 4.

[0039] The front end of the suction nozzle 4 is aligned with the nerve infected immune cell sample to be sucked, the vacuum pump is started by controlling the button, the negative pressure suction pipe of the vacuum pump is connected with the middle segment of the outer side of the suction handle 11, the negative pressure is generated in the suction device 1, the cell sample enters the inner cavity of the cell filter 3 under the action of the negative pressure, after the cell sample enters the cell filter 3, it first passes through the filter membrane 32, the filter membrane 32 has a plurality of holes for filtering oversized cells and adherent double cells, the holes block the oversized cells and adherent double cells in front of the filter membrane 32, and the required immune cells continue to move backward through the filter membrane 32; in the cell filter 3, the front end magnetic ring 35 and the rear end magnetic ring 34 are magnetically adsorbed to each other, and are in close contact with the front and rear surfaces of the silica gel ring 33, the silica gel ring 33 plays a role in supporting and fixing the filter membrane 32, and guarantees the stability of the filter membrane 32 when the cell sample passes; the filtered immune cells continue to move backward, enter the inside of the suction handle 11 through the suction end 12, and are collected into the corresponding collection device through the negative pressure suction pipe of the vacuum pump connected with the middle segment of the outer side of the suction handle 11.

[0040] When the cell suction operation is completed, the vacuum pump is turned off, if the filter membrane 32 needs to be replaced, the sleeve 31 is unscrewed from the outer threaded end nozzle at the front end of the suction end 12, due to the magnetic adsorption force between the front end magnetic ring 35 and the rear end magnetic ring 34, the rear end magnetic ring 34 can be easily taken out from the sleeve 31, then the silica gel ring 33 and the filter membrane 32 are taken out, a new filter membrane 32 is replaced, and the silica gel ring 33, the rear end magnetic ring 34 and the front end magnetic ring 35 are reinstalled, finally the sleeve 31 is screwed on the suction end 12 again for next use.

[0041] The present application is not limited to the above-mentioned best embodiment, any person should know that the structural changes made under the inspiration of the present application, any technical solution with the same or similar to the present application, falls within the protection scope of the present application. Finally, it should be noted that the structure, proportion, size, etc. shown in the drawings of the present specification are only used to cooperate with the content disclosed in the specification, for understanding and reading by those skilled in the art, and are not used to limit the limiting conditions of the implementation of the present application, therefore, any modification of the structure, change of the proportion relationship or adjustment of the size, without affecting the effect and the purpose that can be achieved by the present application, should still fall within the scope of the technical content disclosed by the present application.

Claims

1. A cell suction device for immune cell detection of neuroinfection, comprising a suction device (1), characterized in that: The front end of the suction device (1) is provided with a cell filter (3), the inner cavity of the cell filter (3) is fixedly connected with an expansion and contraction structure (5), the front end of the cell filter (3) is clamped with a suction nozzle (4), and the outer circle of the rear section of the suction device (1) is wrapped with a silica gel anti-skid piece (2). The suction device (1) comprises a suction handle (11), a suction end head (12) fixed at the front end of the suction handle (11), and a control button arranged at the front section of the outer surface of the suction handle (11), and the outer side of the middle section of the suction handle (11) is connected with a negative pressure suction pipe of a vacuum pump. The silica gel anti-skid piece (2) comprises an anti-skid jacket (21), a connecting head (23) fixedly connected to the rear end surface of the anti-skid jacket (21), and a canvas hanging belt (22) fixedly connected to the rear end of the connecting head (23), and a rubber restraint ring (24) is sleeved on the outer surface of the canvas hanging belt (22). The cell filter (3) comprises a sleeve (31) screwed at the front end of the suction end head (12), a front end magnetic ring (35) fixed in the inner cavity of the rear section of the sleeve (31), and a rear end magnetic ring (34) placed in the inner cavity of the rear section of the sleeve (31), and the silica gel ring (33) is clamped between the rear end magnetic ring (34) and the front end magnetic ring (35), and the front side of the silica gel ring (33) is fixed with a filter membrane (32). The expansion and contraction structure (5) comprises a plurality of support rods (51) fixedly connected to the inner cavity side wall of the front section of the sleeve (31), a micro servo motor (52) fixed on the mutually gathered one end of the support rods (51), and a lead screw (53) connected with the output shaft of the micro servo motor (52) through a shaft coupling, a nut (54) movably threaded on the rear section of the outer circle of the lead screw (53), a bearing (55) rotatably arranged at the front end of the outer circle of the lead screw (53), wherein a plurality of groups of X-shaped hinge pieces (57) are hinged on the outer circle of the nut (54) and the bearing (55), X-shaped hinge pieces (57) are respectively hinged on the outer side end of the X-shaped hinge piece (57), and the outer surface of the tile-shaped expansion piece (56) is in contact with the inner cavity side wall of the suction nozzle (4), The X-shaped hinge piece (57) is arranged in a ring array with the central axis of the lead screw (53) as the base point, and the X-shaped hinge piece (57) comprises two intersecting connecting rods, and the front two ends of the X-shaped hinge piece (57) are respectively hinged with the outer surface of the bearing (55) and the inner side front section of the tile-shaped expansion piece (56); wherein the rear two ends of the X-shaped hinge piece (57) are respectively hinged with the outer circle of the nut (54), and the inner side rear section of the tile-shaped expansion piece (56) is slidingly connected; The inner side rear section of the tile-shaped expansion piece (56) is fixedly connected with a sliding groove (58), and the rear outer side end of the X-shaped hinge piece (57) is connected with a sliding block matched with the sliding groove (58); The front section of the outer circle of the sleeve (31) is inserted into the inner cavity rear section area of the suction nozzle (4), and the rear end port of the sleeve (31) is provided with a thread matched with the outer thread end nozzle of the front end of the suction end head (12).

2. The cell suction device for immune cell detection of nerve infection according to claim 1, characterized in that: The front end of the suction end head (12) is provided with a threaded end nozzle, which is used for connecting the rear end of the sleeve (31) with the suction end head (12) through the threaded end nozzle.

3. The cell suction device for immune cell detection of nerve infection according to claim 1, characterized in that: The outer surface of the anti-skid sleeve (21) is provided with a concave surface matching the curve of the human hand, and the outer side wall of the anti-skid sleeve (21) is provided with openings for the control button and the negative pressure pipe.

4. The cell suction device for immune cell detection of nerve infection according to claim 1, characterized in that: The edges of the canvas hanging belt (22) are all chamfered, and the inner surface of the canvas hanging belt (22) is in contact with the human wrist, and the inner and outer side walls of the rubber restraint ring (24) are all provided with multiple grooves for increasing the friction with the outer surface of the canvas hanging belt (22) to realize the size restriction of the opening and closing of the rear section of the canvas hanging belt (22).

5. The cell suction device for immune cell detection of nerve infection according to claim 1, characterized in that: The front end magnetic ring (35) and the rear end magnetic ring (34) are magnetically attracted to each other, and the side walls of the front end magnetic ring (35) and the rear end magnetic ring (34) close to each other are in close contact with the front and rear surfaces of the silica gel ring (33).

6. The cell suction device for immune cell detection of nerve infection according to claim 1, characterized in that: The periphery of the filter membrane (32) is provided with multiple holes for filtering large cells and adhering double cells.

7. The cell suction device for immune cell detection of nerve infection according to claim 1, characterized in that: The middle and rear section of the lead screw (53) is provided with threads matched with the lead nut (54).

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