Bio-organic fertilizer rich in deep sea enzymolysis fish protein and preparation method of bio-organic fertilizer

Abstract

The invention relates to the technical field of fertilizer preparation, and particularly discloses a biological organic fertilizer rich in deep sea enzymolysis fish protein and a preparation method of the biological organic fertilizer. The biological organic fertilizer is prepared from the following components in parts by weight: 35 to 45 parts of organic fertilizer (containing deep sea enzymolysis fish protein, tobacco powder, meat powder and bone meal), 6 to 15 parts of compound microorganism (such as bacillus licheniformis and the like), 5 to 10 parts of medium element fertilizer, 1 to 2 parts of trace element fertilizer, 0.1 to 1 part of xanthan gum and sulfur suspension, 30 to 35 parts of outer layer fertilizer (containing humic acid, urea and the like) and 0.1 to 0.5 part of anti-blocking agent. The preparation method comprises the following steps: separately fermenting microorganisms to prepare bacterial powder; mixing, crushing and granulating the raw materials to form master batches; spraying xanthan gum and sulfur suspension on the surface of the master batch to form a film; spraying the outer-layer fertilizer slurry for several times; and finally, an anti-blocking agent is sprayed, and air drying is conducted. The deep sea enzymolysis fish protein is taken as a core, the balance of quick-acting fertilizer efficiency and slow release is realized, the soil structure can be improved, the crop growth is promoted, the stress resistance and the fertilizer utilization rate are improved, the soil-borne diseases are inhibited, and the method has the advantages of high efficiency, environment friendliness, economy, practicability and the like.

Description

Technical Field

[0001] This invention relates to the field of fertilizer preparation technology, and in particular to a bio-organic fertilizer rich in deep-sea enzymatically hydrolyzed fish protein and its preparation method. Background Technology

[0002] Currently, producing green and efficient organic fertilizer from livestock and poultry manure through aerobic composting and applying it to the soil is an important link in the organic integration of animal husbandry and ecological agriculture. However, a series of problems, such as low fertilizer efficiency, slow results, and difficulty in promotion, seriously restrict the application of organic fertilizers.

[0003] Meanwhile, numerous research institutions both domestically and internationally have been working to address challenges such as organic fertilizer utilization and environmental pollution. Researchers, based on local conditions and the different growth patterns and nutrient requirements of various crops, have developed and promoted the application of slow-release organic compound fertilizers and compound microbial fertilizers, achieving significant social, economic, and ecological benefits. These fertilizers have demonstrated promising effects in terms of long-lasting effectiveness, improved fertilizer utilization, soil loosening, soil fertility enhancement, prevention of soil-borne pests and diseases, and increased fruit quality and yield. Especially with the gradual decrease in rural labor, the labor-saving and convenient "one-step base fertilizer application" method is highly welcomed by farmers.

[0004] However, research results show that the release of inorganic nutrients in bio-organic fertilizers is closely related to the type, quantity, fineness of organic materials, and the degree to which inorganic nutrients are adsorbed by organic matter after dissolving during production. The higher the specific gravity, content, and fineness of the organic materials, and the more inorganic nutrients are adsorbed by the organic matter, the slower the fertilizer disintegrates, the longer the nutrient release is delayed, and the more significant the slow-release effect of the fertilizer. Conversely, the fertilizer's rapid effectiveness decreases. Therefore, the poor rapid effectiveness of nutrients in organic fertilizers and compound microbial fertilizers severely restricts their development and affects their application. Nevertheless, the slow-release characteristics of bio-organic fertilizers play an important role in reducing the amount and loss of chemical fertilizers, slowing the release rate of inorganic nutrients in fertilizers, delaying the nutrient release time, and reducing the labor required for topdressing in crop cultivation. How to improve the utilization rate of bio-organic fertilizer products, prevent or inhibit various soil-borne diseases, increase yield, improve quality, and accelerate the replacement of chemical fertilizers with bio-organic fertilizers are urgent problems to be solved. Summary of the Invention

[0005] This invention aims to address the shortcomings of existing technologies by providing a bio-organic fertilizer rich in deep-sea enzymatically hydrolyzed fish protein and its preparation method. This fertilizer uses deep-sea enzymatically hydrolyzed fish protein as its core functional component, combined with various organic and inorganic raw materials, to achieve a balance between rapid and slow-release fertilizer effects.

[0006] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a bio-organic fertilizer rich in deep-sea enzymatically hydrolyzed fish protein, by weight: The mixture contains 35-45 parts organic fertilizer, 6-15 parts compound microorganisms, 5-10 parts medium-element fertilizer, 1-2 parts micro-element fertilizer, 0.1-1 parts xanthan gum and sulfur suspension, 30-35 parts outer layer fertilizer, and 0.1-0.5 parts anti-caking agent; the organic fertilizer includes tobacco powder, meat meal, bone meal and deep-sea enzymatically hydrolyzed fish protein, with deep-sea enzymatically hydrolyzed fish protein as the key active ingredient.

[0007] Preferably, the composite microorganisms include Bacillus licheniformis, Bacillus jellyoidis, Bacillus subtilis, and Bacillus amyloliquefaciens.

[0008] Preferably, the xanthan gum and sulfur suspension comprises xanthan gum, sulfur powder, and bentonite.

[0009] Preferably, the outer fertilizer comprises humic acid powder, urea, ammonium sulfate, potassium chloride, monoammonium phosphate, magnesium sulfate, zinc sulfate, boric acid, amino acids, and potassium humate.

[0010] Preferably, the anti-caking agent is an alkylamine acetate cationic surfactant.

[0011] This invention uses deep-sea enzymatically hydrolyzed fish protein as its core functional component. Deep-sea enzymatically hydrolyzed fish protein is rich in small-molecule fish protein peptides, free amino acids, vitamins, biopolysaccharides, nucleotides, betaine, taurine, and complex mineral elements, among other organic nutrients. After being applied to the soil, it can be rapidly utilized by beneficial microorganisms, promoting microbial reproduction, optimizing soil structure, and improving the soil's water and fertilizer retention capacity. Its nutrients can regulate and balance plant growth and development, promoting the balanced absorption of required nutrients by crops and enhancing photosynthetic efficiency. Simultaneously, it increases the content of unsaturated fatty acids and proline in crop membrane lipids, stabilizing membrane structure and improving the crop's cold resistance. It exhibits outstanding performance in activating the soil, preventing nutrient loss, and improving the utilization rate of chemical fertilizers by plants.

[0012] This invention also selects tobacco powder, meat meal, and bone meal as organic carriers. Tobacco powder is rich in organic matter and various nutrients, such as organic carbon, total nitrogen, total phosphorus, and potassium, which can improve soil fertility and water content, and improve soil structure. Meat meal contains abundant crude protein, organic nitrogen, phosphorus, potassium, and other elements, providing essential nutrients for crops. Bone meal is rich in nutrients such as phosphorus, calcium, magnesium, and iron, promoting plant root development and disease and pest resistance. These components work synergistically with deep-sea enzymatically hydrolyzed fish protein to enhance fertilizer efficiency.

[0013] This invention incorporates a compound of microorganisms (Bacillus licheniformis, Bacillus mucilaginosus, Bacillus subtilis, and Bacillus amyloliquefaciens) to prepare a high-purity microbial powder through separate fermentation, preserving the metabolic products. When applied to the soil, it improves the rhizosphere microbial community, inhibits harmful pathogens, induces the production of defensive enzymes in crops, and enhances stress resistance.

[0014] This invention stabilizes crop cell membrane structure, stimulates cell elongation, promotes root growth and physiological metabolism, and avoids nutrient deficiency problems by adding medium and trace elements.

[0015] In the preparation process, this invention combines fast-acting and slow-release nutrient engineering by spraying xanthan gum and sulfur suspension, outer fertilizer and anti-caking agent, so that a hydrophobic film is formed on the surface of fertilizer particles to prevent clumping and ensure instant replenishment and continuous absorption of fertilizer.

[0016] This invention also provides a method for preparing bio-organic fertilizer rich in deep-sea enzymatically hydrolyzed fish protein, comprising the following steps: Step 1: Inoculate the inoculum from the compound microorganisms at a rate of 1.0%-2.0% (g / L) into an aqueous solution in a fermenter that has been sterilized at 121℃ for 30 min and then cooled to 35℃. The solution contains 0.5% glucose, 2.0% starch, 1.5% soybean meal, 1% yeast powder, 0.2% magnesium sulfate, 0.3% calcium carbonate, 0.15% dipotassium hydrogen phosphate, 0.03% manganese sulfate, and has a pH of 7.0. Ferment for 50-55 h to obtain fermentation broths of Bacillus licheniformis, Bacillus mucilaginosus, Bacillus subtilis, and Bacillus amyloliquefaciens, respectively. Step 2: Weigh the various bacterial powder raw materials prepared in Step 1, mix them evenly, and make the first mixture; Step 3: Weigh the raw materials of each medium-element fertilizer and trace element raw material, mix them, crush them, pass them through a 100-mesh sieve, add them to the first mixture, and stir them thoroughly to obtain the second mixture. Step 4: Weigh the raw materials of each organic fertilizer (including tobacco powder, meat meal, bone meal and deep-sea enzymatically hydrolyzed fish protein), mix them, crush them, pass them through a 100-mesh sieve, add the second mixture, and stir thoroughly until evenly mixed and ready for use. Step 5: After stirring the material processed in Step 4 at a speed of 50-70 r / min for 20-30 minutes, place it in a granulator for granulation to obtain masterbatch for later use. Step 6: Spray xanthan gum and sulfur suspension onto the surface of the masterbatch fertilizer. Spray 5 kg of xanthan gum and sulfur suspension onto every 1000 kg of masterbatch fertilizer. Then put it into the drying drum for drying. During drying, the inlet temperature is 300℃ and the outlet temperature is 120℃, so that a film is formed on the surface of the masterbatch fertilizer. Step 7: Pour the raw materials of the outer layer fertilizer into a slurry tank containing 100℃ hot water and stir while dissolving to make fertilizer slurry. Adjust the drum speed to control the fertilizer flow rate to 60kg / minute. Spray the outer layer fertilizer slurry onto the masterbatch fertilizer sprayed in Step 6 in three stages. Step 8: Spray the raw material with the outer layer fertilizer sprayed in Step 7 with an alkylamine acetate anti-caking agent. Spray 3 kg of alkylamine acetate anti-caking agent for every 1000 kg of fertilizer. Then put it into the drying drum for drying and cooling, and then package and store it.

[0017] Beneficial effects This invention uses deep-sea enzymatically hydrolyzed fish protein as its core functional component. It has a fast and long-lasting fertilizer effect, can quickly replenish the various organic nutrients required by crops, and achieves continuous nutrient supply through slow-release technology. It is an ideal fertilizer for high-efficiency, high-yield agriculture and green agriculture.

[0018] 1. The organic raw material of this invention is deep-sea enzymatically hydrolyzed fish protein, combined with tobacco powder, meat meal and bone meal, which can activate beneficial soil microorganisms, promote root growth and improve the soil ecological environment.

[0019] 2. The deep-sea enzymatically hydrolyzed fish protein added in this invention is rich in small molecule fish protein peptides, free amino acids and other active substances, which promote the proliferation of beneficial bacteria, stimulate the development of crop roots, make the roots and stems thicker, extend the functional period of leaves, and significantly improve fertilizer utilization.

[0020] 3. This invention fundamentally improves soil structure, making the soil loose and eliminating the need for deep tillage, and enhancing the supply capacity of water, fertilizer, air and heat. It has a significant effect on soil compaction, acidification, salinity and alkali, and plant death caused by continuous cropping.

[0021] 4. This invention has a unique preventive and inhibitory effect on preventing or inhibiting various soil-borne diseases such as ginger wilt.

[0022] 5. This invention combines the long-lasting effect of ordinary organic fertilizer with the rapid effect of ordinary inorganic fertilizer. The release of micronutrients and macronutrients is synchronized, achieving a highly efficient fertilization effect that integrates rapid and slow-release fertilizer effects.

[0023] 6. The present invention has simple steps, high reusability, and low cost, and has high economic value and industrial application prospects. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0025] Example 1 This invention provides a bio-organic fertilizer rich in deep-sea enzymatically hydrolyzed fish protein, by weight: 42 parts organic fertilizer (including tobacco powder, meat meal, bone meal and deep-sea enzymatically hydrolyzed fish protein), 14 parts compound microorganisms, 9 parts medium-element fertilizer, 1.5 parts micro-element fertilizer, 0.5 parts xanthan gum and sulfur suspension, 32.5 parts outer layer fertilizer, and 0.5 parts anti-caking agent.

[0026] Preferably, the composite microorganisms include Bacillus licheniformis, Bacillus jellyoidis, Bacillus subtilis, and Bacillus amyloliquefaciens.

[0027] Preferably, the xanthan gum and sulfur suspension comprises xanthan gum, sulfur powder, and bentonite.

[0028] Preferably, the outer fertilizer comprises humic acid powder, urea, ammonium sulfate, potassium chloride, monoammonium phosphate, magnesium sulfate, zinc sulfate, boric acid, amino acids, and potassium humate.

[0029] Preferably, the anti-caking agent is an alkylamine acetate cationic surfactant.

[0030] Example 2 This invention also provides a method for preparing bio-organic fertilizer rich in deep-sea enzymatically hydrolyzed fish protein, comprising the following steps: Step 1: Inoculate the inoculum from the compound microorganisms at a rate of 1.5% (g / L) into an aqueous solution in a fermenter that has been sterilized at 121℃ for 30 min and then cooled to 35℃. The solution contains 0.5% glucose, 2.0% starch, 1.5% soybean meal, 1% yeast powder, 0.2% magnesium sulfate, 0.3% calcium carbonate, 0.15% dipotassium hydrogen phosphate, 0.03% manganese sulfate, and has a pH of 7.0. Ferment for 52 h to obtain fermentation broths of Bacillus licheniformis, Bacillus colloidis, Bacillus subtilis, and Bacillus amyloliquefaciens, respectively. Step 2: Weigh the various bacterial powder raw materials prepared in Step 1, mix them evenly, and make the first mixture; Step 3: Weigh the raw materials of each medium-element fertilizer and trace element raw material, mix them, crush them, pass them through a 100-mesh sieve, add them to the first mixture, and stir them thoroughly to obtain the second mixture. Step 4: Weigh the raw materials of each organic fertilizer (including tobacco powder, meat meal, bone meal and deep-sea enzymatically hydrolyzed fish protein), mix them, crush them, pass them through a 100-mesh sieve, add the second mixture, and stir thoroughly until evenly mixed and ready for use. Step 5: After stirring the material processed in Step 4 at a speed of 55 r / min for 25 min, place it in a granulator for granulation to obtain masterbatch for later use. Step 6: Spray xanthan gum and sulfur suspension onto the surface of the masterbatch fertilizer. Spray 5 kg of xanthan gum and sulfur suspension onto every 1000 kg of masterbatch fertilizer. Then put it into the drying drum for drying. During drying, the inlet temperature is 300℃ and the outlet temperature is 120℃, so that a film is formed on the surface of the masterbatch fertilizer. Step 7: Pour the raw materials of the outer layer fertilizer into a slurry tank containing 100℃ hot water and stir while dissolving to make fertilizer slurry. Adjust the drum speed to control the fertilizer flow rate to 60kg / minute. Spray the outer layer fertilizer slurry onto the masterbatch fertilizer sprayed in Step 6 in three stages. Step 8: Spray the raw material with the outer layer fertilizer sprayed in Step 7 with an alkylamine acetate anti-caking agent. Spray 3 kg of alkylamine acetate anti-caking agent for every 1000 kg of fertilizer. Then put it into the drying drum for drying and cooling, and then package and store it.

[0031] The embodiments described above are merely illustrative of implementation methods of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A bio-organic fertilizer rich in deep-sea enzymatically hydrolyzed fish protein, characterized in that, The fertilizer comprises the following components by weight: 35-45 parts organic fertilizer, 6-15 parts compound microorganisms, 5-10 parts medium-element fertilizer, 1-2 parts micro-element fertilizer, 0.1-1 parts xanthan gum and sulfur suspension, 30-35 parts outer layer fertilizer, and 0.1-0.5 parts anti-caking agent; the organic fertilizer includes tobacco powder, meat meal, bone meal and deep-sea enzymatically hydrolyzed fish protein, wherein deep-sea enzymatically hydrolyzed fish protein is the key active ingredient.

2. The bio-organic fertilizer according to claim 1, characterized in that, The composite microorganisms include Bacillus licheniformis, Bacillus jellyoidis, Bacillus subtilis, and Bacillus amyloliquefaciens.

3. The bio-organic fertilizer according to claim 1, characterized in that, The xanthan gum and sulfur suspension includes xanthan gum, sulfur powder, and bentonite.

4. The bio-organic fertilizer according to claim 1, characterized in that, The outer fertilizer includes humic acid powder, urea, ammonium sulfate, potassium chloride, monoammonium phosphate, magnesium sulfate, zinc sulfate, boric acid, amino acids, and potassium humate.

5. The bio-organic fertilizer according to claim 1, characterized in that, The anti-caking agent is an alkylamine acetate cationic surfactant.

6. A method for preparing a bio-organic fertilizer rich in deep-sea enzymatically hydrolyzed fish protein as described in any one of claims 1-5, characterized in that, Includes the following steps: Step 1: Inoculate the strains of the compound microorganisms into the fermentation medium for fermentation to obtain the fermentation broth of each strain; Step 2: Mix the various bacterial powder raw materials prepared in Step 1 evenly to form the first mixture; Step 3: Mix and crush the medium-quantity fertilizer and micro-quantity raw materials, then add them to the first mixture and stir evenly to obtain the second mixture; Step 4: After mixing and crushing the raw materials of the organic fertilizer, add the second mixture and stir evenly; Step 5: Granulate the material from Step 4 to obtain masterbatch; Step 6: Spray xanthan gum and sulfur suspension onto the surface of the masterbatch, and then dry to form a film; Step 7: Prepare the raw materials of the outer fertilizer into fertilizer slurry and spray it onto the masterbatch from Step 6 in several stages; Step 8: Spray anti-caking agent, air dry and cool, then seal.

7. The method according to claim 6, characterized in that, In step one, the fermentation medium contains 0.5% glucose, 2.0% starch, 1.5% soybean meal, 1% yeast powder, 0.2% magnesium sulfate, 0.3% calcium carbonate, 0.15% dipotassium hydrogen phosphate, and 0.03% manganese sulfate, with a pH of 7.

0. The fermentation conditions are: inoculum concentration of 1.0%-2.0% (g / L), fermentation temperature of 35℃, and fermentation time of 50-55 h.

8. The method according to claim 6, characterized in that, In step six, 5 kg of xanthan gum and sulfur suspension are sprayed on every 1000 kg of masterbatch. The inlet temperature is 300℃ and the outlet temperature is 120℃ during drying.

9. The method according to claim 6, characterized in that, In step seven, the outer fertilizer slurry is made by dissolving the outer fertilizer raw materials in hot water at 100°C and spraying it onto the masterbatch in three stages, with the fertilizer flow rate controlled at 60 kg / min.

10. The method according to claim 6, characterized in that, In step eight, spray 3 kg of anti-caking agent for every 1000 kg of fertilizer.