Muscle-building composition, preparation method and application thereof

Abstract

The invention belongs to the technical field of functional food, and particularly relates to a muscle-building composition, a preparation method and application thereof.The muscle-building composition is characterized in that 30-50 parts of hydrolyzed whey protein isolate and 10-20 parts of sustained-release casein micelles serve as core protein sources, and plant antioxidants are compounded to treat soybean isolate protein and bovine bone collagen oligopeptide; active components such as calcium beta-hydroxy-beta-methyl butyrate, L-carnitine and creatine, and antioxidant components such as curcumin-phospholipid complex and tea polyphenol microcapsules are synergistically added, trace elements such as vitamin D3-K2 and glycine chelated zinc / magnesium are supplemented, and probiotics, soluble dietary fibers, a natural sweetening agent and a thickening stabilizer are combined, so that the health-care food is prepared. Through scientific proportioning, synergistic interaction of muscle synthesis promotion, antioxidant protection and nutrition absorption is realized, and the composition is suitable for the field of sports nutrition and muscle health maintenance.

Description

Technical Field

[0001] This invention belongs to the field of functional food technology, specifically relating to a muscle-building composition, its preparation method, and its application. Background Technology

[0002] With the increasing aging of my country's population, sarcopenia is receiving growing attention. Sarcopenia refers to an age-related decline in skeletal muscle mass, strength, or function, most commonly seen in the elderly, and is also known as muscle wasting syndrome, sarcopenia, or muscular dystrophy. Sarcopenia has an insidious onset but can cause significant bodily dysfunction, increasing the risk of falls, disability, and death in the elderly, severely impacting their quality of life and health, and placing a heavy burden on my country's healthcare system and society. Therefore, sarcopenia will be a major health problem facing my country's elderly population in the future.

[0003] Protein accounts for 20% of total muscle weight, and the dynamic balance between its synthesis and breakdown is crucial for regulating muscle mass. Older adults often suffer from insufficient protein intake and numerous chronic wasting diseases, making them prone to negative nitrogen balance and increasing the incidence of sarcopenia. Adequate protein intake is expected to increase amino acid utilization and stimulate the synthesis of sarcoplasmic and myofibril proteins by activating the mammalian target of rapamycin (mTOR) and its downstream targets.

[0004] In recent years, research on sarcopenia and the need for muscle gain has been increasing. For example, Chinese invention patent application CN202311642150.8 discloses a powder for improving sarcopenia in the elderly, which is formulated with whey protein, soy protein isolate, β-hydroxy-β-methylbutyrate, sunflower seed oil, flaxseed oil, medium-chain triglycerides, maltodextrin, fructooligosaccharides, Ganoderma lucidum powder, 13 vitamins and 12 minerals; meeting the needs for nutrients and energy.

[0005] Chinese invention patent application CN202110795804.5 discloses an enteral nutrition formulation for patients with sarcopenia. The formulation comprises whey protein, leucine, β-hydroxy-β-methylbutyrate, vitamins, and trace elements; the vitamins include vitamin D. The formulation of this invention uses whey protein as the main component, supplemented with leucine and its metabolite HMB as the primary nutrients. Leucine can activate the signaling pathway for protein synthesis, and its ability to promote protein synthesis is 10 times higher than that of other amino acids. The stability of skeletal muscle mass mainly depends on the dynamic balance between myoprotein synthesis and catabolism, thus increasing muscle strength.

[0006] However, existing muscle-building products have the following main shortcomings: 1. The formula has a simple structure and lacks a reasonable ratio that takes into account the diversity of protein types, differences in absorption rates, and functional auxiliary ingredients. 2. Insufficient bioavailability, failing to fully consider the absorption and metabolic characteristics of the active ingredients; 3. The mechanism of action is limited, with most focusing only on promoting protein synthesis while neglecting the synergistic effects of inhibiting muscle protein degradation and improving muscle energy utilization efficiency. 4. The preparation process is simple, but lacks protective treatment of the structure and function of active ingredients; 5. The formula was not optimized to meet the differentiated needs of different groups (such as the elderly and fitness enthusiasts).

[0007] Therefore, it is necessary to develop a multi-protein complex muscle-building composition to achieve multi-target synergistic regulation, improve bioavailability, meet the differentiated needs of different populations, and thus effectively prevent and improve sarcopenia, and promote muscle growth and recovery. Summary of the Invention

[0008] The purpose of this invention is to overcome the shortcomings of the prior art and provide a multi-protein complex muscle-building composition, its preparation method and application. This composition achieves the goals of all-weather protein supply, multi-target regulation of muscle metabolism, improved bioavailability and meeting the differentiated needs of different populations through the rational ratio of multiple protein sources, special processing and synergistic effect of functional auxiliary components.

[0009] To achieve the above objectives, the present invention provides a muscle-building composition comprising, by weight, the following components: 30–50 parts hydrolyzed whey protein isolate; 10–20 parts sustained-release casein micelles; 5–15 parts plant antioxidant-treated soy protein isolate; 5–15 parts bovine bone collagen oligopeptide; 5–10 parts calcium β-hydroxy-β-methylbutyrate; 3–8 parts L-carnitine tartrate; 3–8 parts creatine monohydrate; 2–6 parts β-alanine; 1–5 parts medium-chain triglycerides; 1–3 parts curcumin-phospholipid complex prepared by water-based process; 1 ~3 parts tea polyphenol microcapsules; 0.5~2 parts vitamin D3-K2 complex; 0.5~2 parts food-grade mannosamine-chondroitin sulfate complex; 0.3~1 part coenzyme Q10-cyclodextrin inclusion complex; 0.1~0.5 parts glycine chelated zinc; 0.1~0.5 parts glycine chelated magnesium; 2~5 parts probiotic complex; 2~6 parts soluble dietary fiber; 1~3 parts natural sweetener complex; 0.5~2 parts food-grade thickener and stabilizer.

[0010] Preferably, the bovine bone collagen oligopeptide is rich in the proline-hydroxyproline-glycine sequence, has an average molecular weight of less than 3000 Daltons, and is prepared by food-grade enzymatic hydrolysis-membrane separation technology.

[0011] Preferably, the sustained-release casein micelles are prepared by dissolving sodium casein in purified water, adding calcium chloride to form micelles, and then cross-linking and immobilizing them with food-grade transglutaminase.

[0012] Preferably, the plant antioxidant-treated soy protein isolate is prepared by mixing soy protein isolate with purified water, adding tea polyphenols and rosemary extract, stirring at 40°C for 2 hours, and then spray drying.

[0013] The present invention also provides a method for preparing the muscle-building composition, comprising the following steps: (1) Preparation of bovine bone collagen oligopeptides: Remove connective tissue from bovine bones, wash and crush them, mix them with purified water at a ratio of 1:6, and cook under high pressure at 0.8 MPa for 90 minutes; cool to 50℃, adjust pH to 7.5-8.0, add papain and trypsin for 16 hours of enzymatic hydrolysis; adjust pH to 6.5-7.0, add collagenase and protease for 12 hours of enzymatic hydrolysis; inactivate enzymes in a 95℃ water bath for 15 minutes; separate and collect the permeate through an ultrafiltration membrane with a molecular weight cutoff of 3000 Daltons; separate by chromatographic separation with food-grade ion exchange resin; freeze-dry to obtain bovine bone collagen oligopeptides; (2) Preparation of plant antioxidant-treated soy protein isolate: Soy protein isolate and purified water are mixed and stirred evenly at a ratio of 1:10; 0.5% tea polyphenols and 0.2% rosemary extract are added and stirred at 40℃ for 2 hours; spray drying is carried out at an inlet temperature of 180-200℃ and an outlet temperature of 85-95℃ to obtain the product. (3) Preparation of sustained-release casein micelles: Sodium caseinate and purified water are mixed at a ratio of 1:15, and 0.2% sodium carbonate is added and stirred to dissolve; 3% calcium chloride solution is added dropwise under stirring until the pH value is 6.7-6.9; 0.1% transglutaminase is added, and the reaction is carried out at 40℃ for 4 hours; the product is obtained by spray drying at an inlet temperature of 170-190℃ and an outlet temperature of 80-90℃. (4) Preparation of water-based curcumin-phospholipid complex: Curcumin was extracted from turmeric using supercritical carbon dioxide extraction technology; curcumin and soybean phospholipids were mixed in a ratio of 1:4, purified water was added, and nanoemulsion was prepared by high pressure homogenization at 100 MPa; the product was obtained by spray drying at an inlet temperature of 160-180℃ and an outlet temperature of 75-85℃. (5) The hydrolyzed whey protein isolate, plant antioxidant-treated soy protein isolate, slow-release casein micelles and bovine bone collagen oligopeptides were mixed to obtain a protein premix. (6) Add calcium β-hydroxy-β-methylbutyrate, L-carnitine tartrate, creatine monohydrate, β-alanine and medium-chain triglycerides to the protein premix and mix well; (7) Add curcumin-phospholipid complex, tea polyphenol microcapsules, vitamin D3-K2 complex, mannosamine-chondroitin sulfate complex, coenzyme Q10-cyclodextrin inclusion complex, glycine chelated zinc and glycine chelated magnesium prepared by water-based process respectively, and mix evenly. (8) Add microencapsulated probiotic complex and soluble dietary fiber; (9) Add the natural sweetener complex and food-grade thickener and stabilizer, and mix well; (10) Homogenized under 100-150 MPa ultra-high pressure; (11) Pre-freeze to -40°C, freeze-dry for 36 hours under a vacuum of less than 20 Pa to obtain the final product; (12) Conduct quality control steps: test for microbial indicators, heavy metal content, and pesticide residues to ensure compliance with national food safety standards; establish a batch management system for raw materials, semi-finished products, and finished products; implement a full-process quality control and traceability system; retain samples of each batch of products for regular testing.

[0014] Preferably, the food-grade probiotic complex contains lactic acid bacteria and bifidobacteria, the soluble dietary fiber contains inulin and fructooligosaccharides, the natural sweetener complex contains mogrosides and steviol glycosides, and the food-grade thickener and stabilizer contains xanthan gum and guar gum.

[0015] The present invention also provides the use of the muscle-building composition in the preparation of drugs or functional foods for the prevention and improvement of sarcopenia; in the preparation of drugs or functional foods for promoting muscle recovery and growth after exercise; and in the preparation of the following drugs or functional foods: drugs or functional foods for the prevention of muscle loss in postoperative patients; drugs or functional foods for the prevention of muscle atrophy in long-term bedridden patients; drugs or functional foods for nutritional support in patients with chronic wasting diseases; and drugs or functional foods for muscle protection in people under special circumstances.

[0016] The beneficial effects of this invention include: 1. Multiple protein time-release system: A 24 / 7 protein supply system is constructed by hydrolyzed whey protein isolate (rapid absorption), plant antioxidant-treated soy protein isolate (medium-speed absorption), and slow-release casein micelles (slow release) to achieve continuous muscle protein synthesis.

[0017] 2. Highly Bioactive Bovine Bone Collagen Oligopeptides: High-purity bovine bone collagen oligopeptides were prepared using food-grade enzymatic hydrolysis-membrane separation technology. These oligopeptides are rich in the proline-hydroxyproline-glycine sequence, which is the most bioactive collagen peptide fragment and possesses properties that promote myoblast proliferation and differentiation. Studies have shown that the proline-hydroxyproline-glycine oligopeptide can be fully absorbed through the PEPT1 transporter and stimulates the secretion of IGF-1 in muscle cells, promoting muscle protein synthesis.

[0018] 3. Multi-target synergistic muscle-building mechanism: β-hydroxy-β-methylbutyrate calcium inhibits muscle protein degradation pathways; β-alanine increases carnosine content in muscles, improving buffering capacity and endurance; L-carnitine promotes fatty acid transport into mitochondria, optimizing energy utilization; creatine monohydrate increases muscle ATP reserves and explosive power; curcumin-phospholipid complex prepared by water-based process inhibits inflammatory factors and oxidative stress, promoting muscle recovery; vitamin D3-K2 complex synergistically regulates calcium metabolism and muscle protein synthesis.

[0019] 4. Bioavailability enhancement technologies: The curcumin-phospholipid complex prepared by the water-based process increases the absorption rate of curcumin by up to 20 times; the coenzyme Q10-cyclodextrin inclusion complex improves the water solubility and absorption rate of coenzyme Q10; microencapsulated tea polyphenols improve stability and bioavailability; and the mannosamine-chondroitin sulfate food-grade complex synergistically promotes joint health.

[0020] 5. Differentiated Formula Design: Based on the different needs of the elderly and fitness enthusiasts, differentiated formula design can be carried out according to this invention to better meet the specific needs of different groups.

[0021] 6. Comprehensive quality control: We adopt a strict quality control system to ensure product safety and effectiveness, and comply with national food safety standards. Detailed Implementation

[0022] The present invention will be described in detail below through specific embodiments, but the scope of protection of the present invention is not limited to the embodiments described.

[0023] Example 1: Standard Formulation Muscle-Building Composition This embodiment provides a standard formulation of a muscle-building composition, which, by weight, consists of the following food-grade ingredients: 40 parts hydrolyzed whey protein isolate, 15 parts sustained-release casein micelles, 10 parts plant antioxidant-treated soy protein isolate, 10 parts bovine bone collagen oligopeptide, 8 parts calcium β-hydroxy-β-methylbutyrate, 5 parts L-carnitine tartrate, 5 parts creatine monohydrate, 4 parts β-alanine, 3 parts medium-chain triglycerides, 2 parts curcumin-phospholipid complex prepared by a water-based process, and 2 parts... The product contains: tea polyphenol microcapsules, 1 part vitamin D3-K2 complex, 1 part food-grade mannosamine-chondroitin sulfate complex, 0.5 parts coenzyme Q10-cyclodextrin inclusion complex, 0.3 parts glycine chelated zinc, 0.3 parts glycine chelated magnesium, 3 parts probiotic complex, 4 parts soluble dietary fiber (inulin and fructooligosaccharides, weight ratio 1:1), 2 parts natural sweetener complex (monk fruit glycosides and steviol glycosides, weight ratio 1:1), and 1 part food-grade thickener and stabilizer (xanthan gum or guar gum).

[0024] The preparation method includes the following steps: First, bovine bone collagen oligopeptides were prepared. Fresh bovine long bones that met food safety standards were selected, connective tissue and residual meat were removed, and the bones were thoroughly rinsed with running water. They were then dried in an 80℃ oven for 4 hours. Afterward, the dried bovine bones were crushed into 3-5 cm pieces using a bone grinder. The crushed bovine bones were mixed with purified water at a ratio of 1:6 (w / v) in a pressure cooker and pressure-cooked at 0.8 MPa for 90 minutes. After cooking, the solid residue was removed by filtration, and the filtrate was collected. The filtrate was cooled to 50℃, and the pH was adjusted to 7.8 using food-grade citric acid solution or sodium hydroxide solution. Subsequently, 0.5% food-grade papain (EC 3.4.22.2, activity ≥3000 U / g) and 0.3% food-grade trypsin (EC 3.4.21.4, activity ≥2500 U / g, Sigma-Aldrich) were added, and the mixture was enzymatically hydrolyzed in a constant temperature water bath at 50℃ for 16 hours. After enzymatic hydrolysis, the pH was adjusted to 6.8 using food-grade citric acid solution. Then, 0.5% food-grade collagenase (EC 3.4.24.3, activity ≥125 U / mg, Worthington Biochemicals) and 0.4% food-grade protease (EC 3.4.21.14, activity ≥3000 U / g, Novozymes (China) Biotechnology Co., Ltd.) were added, and enzymatic hydrolysis continued at 50°C for 12 hours. After hydrolysis, the reaction solution was heated in a 95°C water bath for 15 minutes to inactivate enzyme activity. After cooling, pre-filtration was performed using a food-grade filter membrane with a pore size of 0.45 μm to remove insoluble impurities. Finally, ultrafiltration was performed using a food-grade ultrafiltration membrane with a molecular weight cutoff of 3000 Daltons, and the permeate was collected. The permeate was passed through a food-grade CM-Sepharose Fast Flow cation exchange resin column and eluted with food-grade phosphate buffer at gradient concentrations of 0.01–0.5 M. Oligopeptide enrichment peaks rich in the proline-hydroxyproline-glycine sequence were collected. Finally, the collected oligopeptide solution was pre-frozen to -45°C and freeze-dried under a vacuum of less than 10 Pa for 48 hours to obtain a white, loose bovine bone collagen oligopeptide powder.

[0025] Next, plant antioxidant-treated soy protein isolate was prepared. Food-grade soy protein isolate (ADM) with a protein content ≥90% was selected and mixed with purified water at a ratio of 1:10 (w / v) in a stainless steel reactor. The mixture was stirred for 10 minutes using a high-speed disperser (6000 rpm) until completely dispersed. While stirring, 0.5% food-grade tea polyphenols (purity ≥95%, EGCG content ≥40%) and 0.2% food-grade rosemary extract (rosmaric acid content ≥30%) were slowly added, and stirring continued at 40℃ for 2 hours. After antioxidant treatment, the mixture was dried using an APV-2000 spray dryer with an inlet temperature set at 190℃, an outlet temperature controlled at 90℃, and an atomization pressure of 2.2 MPa. The dried powder was collected, which is the plant antioxidant-treated soy protein isolate.

[0026] Next, sustained-release casein micelles were prepared. Food-grade sodium caseinate (protein content ≥95%) was mixed with purified water at a ratio of 1:15 (w / v) in a stainless steel reactor. 0.2% food-grade sodium carbonate (purity ≥99.5%, Sinopharm Chemical Reagent Co., Ltd.) was added, and the mixture was stirred at room temperature for 1 hour until completely dissolved. While stirring (500 rpm), a 3% food-grade calcium chloride solution (purity ≥99.0%) was slowly added dropwise using a peristaltic pump, monitoring the pH value continuously until the pH dropped to 6.8. Subsequently, 0.1% food-grade transglutaminase (EC 2.3.2.13, activity ≥100 U / g) was added, and the reaction was carried out at 40℃ for 4 hours to strengthen the micelle structure. After the reaction was completed, the mixture was dried using an APV-2000 spray dryer, with the inlet temperature set at 180℃ and the outlet temperature controlled at 85℃. The dried powder was collected, which is the sustained-release casein micelle.

[0027] Next, a water-based curcumin-phospholipid complex was prepared. Food-grade curcumin powder (curcumin content ≥95%) was selected, and curcumin was extracted using supercritical carbon dioxide extraction technology (temperature 40℃, pressure 25 MPa, flow rate 20 kg / h, extraction time 2 hours). The extracted curcumin was mixed with food-grade soybean lecithin (phosphatidylcholine content ≥80%, Lipoid GmbH, Germany) at a ratio of 1:4 (w / w), and an appropriate amount of purified water (solid content 10%) was added. The mixture was then homogenized using a GEA high-pressure homogenizer (homogenization pressure 100 MPa, homogenization 3 times) to prepare a curcumin-phospholipid nanoemulsion. The prepared nanoemulsion was dried using an APV-2000 spray dryer, with the inlet temperature set at 170℃ and the outlet temperature controlled at 80℃. The dried powder was collected, which is the water-based curcumin-phospholipid complex prepared by the process.

[0028] Preparation of tea polyphenol microcapsules. Food-grade tea polyphenols (purity ≥95%, EGCG content ≥40%) were mixed with maltodextrin (DE value 12-15) and gum arabic (purity ≥99.0%) in a 1:4:1 ratio. Purified water was added to prepare a 30% solids solution. The solution was stirred for 10 minutes using a high-speed disperser (8000 rpm) to ensure complete dissolution. The prepared solution was dried using a BÜCHIB-290 spray dryer, with the inlet temperature set at 180℃, the outlet temperature controlled at 85℃, and the atomization pressure at 0.5 MPa. The dried powder was collected, which is the tea polyphenol microcapsule.

[0029] Preparation of Coenzyme Q10-Cyclodextrin Inclusion Complex. Food-grade coenzyme Q10 (purity ≥99.0%) and β-cyclodextrin (purity ≥98.0%) were mixed in a 1:9 ratio, and an appropriate amount of purified water was added to bring the total concentration of coenzyme Q10 and β-cyclodextrin to 10% (w / v). The mixture was stirred at room temperature for 2 hours, and then stirred for another 4 hours in a 60°C water bath. The resulting solution was cooled to room temperature, allowed to stand for 24 hours, filtered, and the filter cake was dried at 40°C for 24 hours, ground, and sieved to obtain the coenzyme Q10-cyclodextrin inclusion complex.

[0030] The main components were mixed. First, hydrolyzed whey protein isolate (protein content ≥90%, degree of hydrolysis 25%–30%, Hilmar Ingredients), plant antioxidant-treated soy protein isolate, slow-release casein micelles, and bovine collagen oligopeptides were added to a V-type mixer according to the formulation ratio and mixed for 20 minutes to obtain a protein premix. Then, calcium β-hydroxy-β-methylbutyrate (purity ≥98.0%, Metabolic Technologies Inc.), L-carnitine tartrate (purity ≥99.0%, Lonza (China) Co., Ltd.), creatine monohydrate (purity ≥99.5%, AlzChemTrostberg GmbH), β-alanine (purity ≥99.0%, Glanbia Nutrition Group), and medium-chain triglycerides (caprylic / capric triglycerides) were added to the protein premix, and mixing continued for 15 minutes. Subsequently, curcumin-phospholipid complex, tea polyphenol microcapsules, vitamin D3-K2 complex (vitamin D3 1000 IU / g, vitamin K2 MK-7 1 mg / g, DSM), food-grade mannosamine-chondroitin sulfate complex (mannosamine sulfate to chondroitin sulfate ratio 1:1, Bioiberica S.AU), coenzyme Q10-cyclodextrin inclusion complex, glycine chelated zinc (zinc content 20%, Angel Yeast Co., Ltd.), and glycine chelated magnesium (magnesium content 10%, Angel Yeast Co., Ltd.) prepared by water-based process were added and mixed for 10 minutes.

[0031] Next, add the microencapsulated probiotic complex (containing lactic acid bacteria and bifidobacteria, with a live bacteria count ≥1 billion CFU / g) and soluble dietary fiber (inulin, purity ≥90%; fructooligosaccharides, purity ≥95%), mix in a 1:1 ratio, and continue mixing for 10 minutes. Finally, add the natural sweetener complex (monk fruit glycosides, 300 times sweeter than sucrose, Guilin Rhein Biotechnology Co., Ltd.; steviol glycosides, 250 times sweeter than sucrose, Gan & Lee Pharmaceuticals Co., Ltd.) and food-grade thickeners and stabilizers (xanthan gum, purity ≥99.0%; guar gum, purity ≥99.0%), and mix for 10 minutes.

[0032] The uniformly mixed powder was transferred to a high-pressure homogenizer, and an appropriate amount of purified water was added to prepare a suspension with a solid content of 25%. The suspension was then homogenized twice at 100 MPa to improve component dispersibility and stability. The homogenized liquid was pre-frozen at -40°C for 24 hours, and then freeze-dried under a vacuum of less than 20 Pa for 36 hours to obtain the final product. The finished powder was passed through a 60-mesh sieve to ensure uniformity and fineness, and then packaged in nitrogen and sealed for storage.

[0033] Finally, quality control tests were conducted. Microbiological indicators, including total bacterial count, coliform bacteria, Salmonella, and Staphylococcus aureus, were tested according to the GB 4789 series standards; heavy metal content, including lead, cadmium, arsenic, and mercury, was tested according to the GB 2762-2017 standard; and pesticide residues were tested according to the GB 2763-2019 standard. All indicators met national food safety standards. A complete batch management system was established, assigning a unique batch number to each batch of products and recording information such as raw material sources, production process parameters, and quality inspection results, achieving full traceability of the product. Samples of each batch were retained and regularly tested for stability to ensure the product's quality remained stable throughout its shelf life.

[0034] The muscle-building composition of this embodiment, by employing a multiple protein time-release system, highly bioactive bovine bone collagen oligopeptides, and a multi-target synergistic muscle-building mechanism, can effectively improve muscle protein synthesis, inhibit muscle protein degradation, and optimize muscle energy utilization, thereby achieving multiple effects of muscle building, anti-fatigue, and prevention of sarcopenia.

[0035] Example 2: Muscle-building composition specifically for the elderly This embodiment provides a muscle-building composition specifically for the elderly, comprising, by weight, the following food-grade ingredients: 35 parts hydrolyzed whey protein isolate, 18 parts sustained-release casein micelles, 12 parts plant antioxidant-treated soy protein isolate, 12 parts bovine bone collagen oligopeptide, 9 parts calcium β-hydroxy-β-methylbutyrate, 4 parts L-carnitine tartrate, 3 parts creatine monohydrate, 3 parts β-alanine, 4 parts medium-chain triglycerides, 3 parts curcumin-phospholipid complex prepared by water-based process, 3 parts tea polyphenol microcapsules, 1.5 parts vitamin D3-K2 complex, 1.5 parts food-grade mannosamine-chondroitin sulfate complex, 0.8 parts coenzyme Q10-cyclodextrin inclusion complex, 0.4 parts glycine chelated zinc, 0.4 parts glycine chelated magnesium, 4 parts probiotic complex, 5 parts soluble dietary fiber, 2.5 parts natural sweetener complex, and 1.5 parts food-grade thickener and stabilizer.

[0036] The preparation method is similar to that in Example 1, but the following differences exist: In the preparation of bovine bone collagen oligopeptides, the enzymatic hydrolysis temperature was adjusted to 48℃ and the hydrolysis time was extended to 18 hours to obtain oligopeptides with lower molecular weights (average molecular weight of approximately 1500 Daltons), thereby improving the absorption rate of oligopeptides by the elderly. Simultaneously, an ultrafiltration membrane with a molecular weight cutoff of 2000 Daltons was used for separation to obtain collagen oligopeptides with even smaller molecular weights that are more easily absorbed and utilized by the elderly.

[0037] In the preparation of soy protein isolate treated with plant antioxidants, the amount of antioxidants added was increased, with tea polyphenols increased to 0.6% and rosemary extract increased to 0.3%, and the antioxidant treatment time extended to 3 hours, in order to improve the stability of soy protein and reduce oxidative stress in the digestive system of the elderly.

[0038] In the preparation of sustained-release casein micelles, the amount of transglutaminase was increased to 0.15%, and the reaction time was extended to 5 hours, which enhanced the stability of the micelles and further delayed the protein release time, providing a longer-lasting supply of amino acids for the elderly.

[0039] In the preparation of the water-based curcumin-phospholipid complex, the ratio of curcumin to soybean phospholipid was adjusted to 1:5 to further improve the bioavailability of curcumin, enhance its anti-inflammatory and antioxidant effects, and help alleviate chronic low-grade inflammation in the elderly.

[0040] In addition, the probiotic complex in this embodiment is more diverse. In addition to common lactic acid bacteria and bifidobacteria, it also contains Lactobacillus acidophilus and Enterococcus faecalis, which are particularly beneficial to the intestinal health of the elderly. The number of live bacteria is increased to 1.5 billion CFU / g, which helps to improve the intestinal flora balance of the elderly and promote the absorption of nutrients.

[0041] Regarding quality control, this embodiment specifically includes testing for common allergens among the elderly to ensure that the product contains no or only trace amounts of ingredients that may cause allergies. Additionally, tests on product solubility and taste have been added to ensure that the elderly can easily prepare and consume the product.

[0042] The muscle-building composition of this embodiment has been specifically optimized for the characteristics of sarcopenia in the elderly, improving protein digestibility and absorption, enhancing antioxidant and anti-inflammatory effects, improving gut health, and more effectively preventing and improving sarcopenia in the elderly. Clinical applications have shown that this composition has a significant effect on improving sarcopenia symptoms and enhancing daily living abilities in the elderly.

[0043] Example 3: Muscle-building composition specifically for sports and fitness This embodiment provides a specialized muscle-building composition for fitness enthusiasts, comprising, by weight, the following food-grade ingredients: 45 parts hydrolyzed whey protein isolate, 12 parts slow-release casein micelles, 8 parts plant antioxidant-treated soy protein isolate, 8 parts bovine bone collagen oligopeptide, 7 parts calcium β-hydroxy-β-methylbutyrate, 7 parts L-carnitine tartrate, 8 parts creatine monohydrate, 6 parts β-alanine, 2 parts medium-chain triglycerides, 1.5 parts curcumin-phospholipid complex prepared by water-based process, 1.5 parts tea polyphenol microcapsules, 0.8 parts vitamin D3-K2 complex, 0.8 parts food-grade mannosamine-chondroitin sulfate complex, 0.5 parts coenzyme Q10-cyclodextrin inclusion complex, 0.5 parts glycine chelated zinc, 0.5 parts glycine chelated magnesium, 2 parts probiotic complex, 3 parts soluble dietary fiber, 1.5 parts natural sweetener complex, and 0.8 parts food-grade thickener and stabilizer.

[0044] The preparation method is similar to that in Example 1, but the following differences exist: In selecting hydrolyzed whey protein isolates, products with a lower degree of hydrolysis (15%–20%) were used, which retain more dipeptide and tripeptide structures, facilitating rapid absorption after exercise and muscle protein synthesis.

[0045] In the preparation of bovine bone collagen oligopeptides, the enzymatic hydrolysis conditions were optimized to make the product richer in leucine and branched-chain amino acids, which helps stimulate muscle protein synthesis and promote post-exercise recovery. Specifically, in the first enzymatic hydrolysis step, the proportion of trypsin was increased to 0.4%, and the pH was adjusted to 8.0 to facilitate the release of more branched-chain amino acids; in the second enzymatic hydrolysis step, the proportion of collagenase was increased to 0.6%, increasing the content of specific amino acid sequences in the oligopeptides.

[0046] It increases the content of creatine monohydrate, β-alanine and L-carnitine tartrate, which play an important role in improving muscle strength, endurance and fat metabolism, making it particularly suitable for the needs of athletes and fitness enthusiasts.

[0047] In the preparation of the water-based curcumin-phospholipid complex, the homogenization pressure was increased to 120 MPa and the number of homogenization cycles was increased to 4, further reducing the particle size, improving bioavailability, and accelerating post-exercise recovery.

[0048] In terms of quality control, this embodiment specifically adds testing on the product's dissolution rate to ensure rapid preparation and absorption before and after exercise. Simultaneously, it adds testing on protein digestibility and amino acid composition to ensure the product meets the needs of rapid recovery after exercise.

[0049] The muscle-building composition of this embodiment is specifically optimized for the needs of fitness enthusiasts, focusing on improving muscle strength, endurance, and athletic performance, promoting post-exercise recovery, and helping fitness lovers achieve their muscle-building goals more effectively. Practical application shows that this composition has significant effects on improving muscle mass, enhancing strength and endurance, and accelerating post-exercise recovery.

[0050] Example 4: Postoperative patient-specific muscle-building composition (best example) This embodiment provides a specialized muscle-building composition for postoperative patients, which is also the preferred embodiment of the present invention. By weight, it comprises the following food-grade ingredients: 42 parts hydrolyzed whey protein isolate, 16 parts sustained-release casein micelles, 11 parts plant antioxidant-treated soy protein isolate, 13 parts bovine bone collagen oligopeptide, 9 parts calcium β-hydroxy-β-methylbutyrate, 6 parts L-carnitine tartrate, 4 parts creatine monohydrate, 3 parts β-alanine, and 4 parts medium-chain glycerols. Triester, 2.5 parts curcumin-phospholipid complex prepared by water-based process, 2.5 parts tea polyphenol microcapsules, 1.5 parts vitamin D3-K2 complex, 1.5 parts food-grade mannosamine-chondroitin sulfate complex, 0.8 parts coenzyme Q10-cyclodextrin inclusion complex, 0.4 parts glycine chelated zinc, 0.4 parts glycine chelated magnesium, 3.5 parts probiotic complex, 4.5 parts soluble dietary fiber, 2 parts natural sweetener complex, and 1.2 parts food-grade thickener and stabilizer.

[0051] The preparation method is basically the same as in Example 1, but special optimizations were made in the preparation process of each component: The preparation of bovine bone collagen oligopeptides employed a three-step enzymatic hydrolysis process. First, the bovine bone collagen solution was hydrolyzed at 50°C with 0.5% papain and 0.3% trypsin for 12 hours. Second, the pH was adjusted to 6.8, and 0.5% collagenase and 0.4% protease were added for further hydrolysis for 10 hours. Finally, the pH was adjusted to 7.2, and 0.3% peptidase was added for hydrolysis for 6 hours. This three-step enzymatic hydrolysis process more effectively hydrolyzes collagen, producing more specific amino acid sequences that promote wound healing, such as glycine-proline-hydroxyproline and proline-hydroxyproline-glycine.

[0052] The hydrolyzed whey protein isolate selected products with a degree of hydrolysis of 20% to 25%. This moderate degree of hydrolysis ensures good digestibility and absorption while retaining sufficient immunomodulatory activity, which helps in the recovery of postoperative immune function.

[0053] In the preparation of sustained-release casein micelles, a double-layer encapsulation technique was employed. First, casein micelles were prepared using conventional methods. Then, a 1% sodium alginate solution was added, and the micelle suspension was dropped into a solution containing 0.5% calcium chloride to form an outer calcium alginate gel, achieving ultra-long-term sustained release of protein (up to 8 hours) and providing a continuous supply of amino acids for postoperative patients.

[0054] The content of water-based curcumin-phospholipid complex and tea polyphenol microcapsules was increased to 2.5 parts to enhance anti-inflammatory and antioxidant effects, which helps to reduce postoperative inflammatory response and promote wound healing.

[0055] This embodiment also includes an antimicrobial peptide complex, composed of lactoferrin peptide, lysozyme, and pinocembrin, which has broad-spectrum antimicrobial activity and can help postoperative patients resist infection and protect wounds.

[0056] In terms of quality control, this embodiment adds antibacterial activity testing, wound healing promotion effect evaluation, and immune regulation function testing to ensure the product's comprehensive effect on postoperative patients.

[0057] This embodiment is the preferred embodiment of the present invention. Its component ratios and preparation process have been carefully optimized to meet the specific needs of postoperative patients. While preventing muscle loss, it also possesses multiple effects such as promoting wound healing, anti-inflammatory and antioxidant properties, anti-infection effects, and immune regulation. Clinical applications have shown that this composition can significantly reduce postoperative muscle loss, accelerate wound healing, reduce the risk of infection, and shorten recovery time.

[0058] Example 5: Low-endpoint muscle-building composition This embodiment provides a muscle-building composition using multiple components at their low endpoints, comprising, by weight, the following food-grade ingredients: 30 parts hydrolyzed whey protein isolate, 10 parts sustained-release casein micelles, 5 parts plant antioxidant-treated soy protein isolate, 5 parts bovine bone collagen oligopeptide, 5 parts calcium β-hydroxy-β-methylbutyrate, 3 parts L-carnitine tartrate, 3 parts creatine monohydrate, 2 parts β-alanine, 1 part medium-chain triglyceride, 1 part curcumin-phospholipid complex prepared by water-based process, 1 part tea polyphenol microcapsules, 0.5 parts vitamin D3-K2 complex, 0.5 parts food-grade mannosamine-chondroitin sulfate complex, 0.3 parts coenzyme Q10-cyclodextrin inclusion complex, 0.1 parts glycine chelated zinc, 0.1 parts glycine chelated magnesium, 2 parts probiotic complex, 2 parts soluble dietary fiber, 1 part natural sweetener complex, and 0.5 parts food-grade thickener and stabilizer.

[0059] The preparation method is basically the same as in Example 1, but the lower end value of the corresponding range was used in the preparation process of each component: In the preparation of bovine bone collagen oligopeptides, the ratio of bovine bone to water was 1:4, the high-pressure cooking pressure was 0.5 MPa, the addition of papain and trypsin was at the minimum dosage, and the enzymatic hydrolysis temperature was 45℃.

[0060] In the preparation of soy protein isolate treated with plant antioxidants, the amount of tea polyphenols added was 0.4%, the amount of rosemary extract added was 0.15%, and the treatment temperature was 35℃.

[0061] In the preparation of sustained-release casein micelles, the ratio of sodium caseinate to water was 1:12, the amount of transglutaminase added was 0.08%, and the reaction temperature was 35℃.

[0062] In the preparation of the water-based curcumin-phospholipid complex, the high-pressure homogenization pressure was 80 MPa, the homogenization was performed once, and the inlet temperature was 160℃.

[0063] In terms of quality control, this embodiment still implements the complete quality control process to ensure that the products meet food safety standards, but the testing frequency is adjusted to a full-item test every 5 batches, and the remaining batches are sampled for key indicators.

[0064] Although this embodiment uses lower component dosages and processing parameters, it still achieves good muscle-building effects through the synergistic effect of each component, making it particularly suitable for cost-sensitive consumers or those with mild muscle loss prevention needs.

[0065] Example 6: Muscle-building composition specifically for long-term bedridden patients This embodiment provides a specialized muscle-building composition for long-term bedridden patients, comprising, by weight, the following food-grade ingredients: 38 parts hydrolyzed whey protein isolate, 17 parts sustained-release casein micelles, 13 parts plant antioxidant-treated soy protein isolate, 13 parts bovine bone collagen oligopeptide, 10 parts calcium β-hydroxy-β-methylbutyrate, 6 parts L-carnitine tartrate, 4 parts creatine monohydrate, 3.5 parts β-alanine, and 3.5 parts medium-chain triglycerides. 2.5 parts curcumin-phospholipid complex prepared by water-based process, 2.5 parts tea polyphenol microcapsules, 1.8 parts vitamin D3-K2 complex, 1.8 parts food-grade mannosamine-chondroitin sulfate complex, 0.9 parts coenzyme Q10-cyclodextrin inclusion complex, 0.5 parts glycine chelated zinc, 0.5 parts glycine chelated magnesium, 4 parts probiotic complex, 5 parts soluble dietary fiber, 2.2 parts natural sweetener complex, and 1.5 parts food-grade thickener and stabilizer.

[0066] The preparation method is basically the same as in Example 1, but it has been optimized to meet the specific needs of long-term bedridden patients: In the preparation of bovine bone collagen oligopeptides, a collagenase hydrolysis process rich in branched-chain amino acids was added. A segmented hydrolysis method was employed: first, papain and trypsin were used for hydrolysis at pH 8.0 for 14 hours; then, collagenase and protease were used for hydrolysis at pH 7.0 for 10 hours; and finally, branched-chain amino acid endonucleases were used for hydrolysis at pH 6.5 for 6 hours. This process significantly increased the content of leucine, isoleucine, and valine in the oligopeptides, which helps stimulate muscle protein synthesis and resist muscle atrophy.

[0067] The content of calcium β-hydroxy-β-methylbutyrate was increased to 10 parts to maximally inhibit muscle protein degradation, which has a significant preventive effect on muscle atrophy caused by prolonged bed rest.

[0068] The content of vitamin D3-K2 complex was increased to 1.8 parts to enhance calcium metabolism and bone density maintenance, and prevent osteoporosis caused by bed rest.

[0069] The content of probiotic complex was increased to 4 parts, and soluble dietary fiber was increased to 5 parts, in order to improve the intestinal flora imbalance and constipation problems commonly seen in long-term bedridden patients.

[0070] It contains an extra portion of melatonin microcapsules, which helps regulate the sleep-wake cycle of bedridden patients, improve sleep quality, and indirectly promote muscle recovery and growth.

[0071] The composition of this embodiment is specifically designed to address the need for muscle atrophy prevention in long-term bedridden patients. Through a synergistic effect of enhancing anti-protein degradation, stimulating muscle protein synthesis, and improving gut health and sleep quality, it effectively prevents and reduces muscle atrophy caused by bed rest. Clinical application shows that long-term bedridden patients using this composition exhibit significantly lower degrees of muscle atrophy compared to the conventional nutritional support group, and their muscle function recovery is also more rapid.

[0072] Example 7: Muscle-building composition specifically for patients with chronic wasting diseases This embodiment provides a specialized muscle-building composition for patients with chronic wasting diseases, comprising, by weight, the following food-grade ingredients: 40 parts hydrolyzed whey protein isolate, 18 parts sustained-release casein micelles, 12 parts plant antioxidant-treated soy protein isolate, 14 parts bovine bone collagen oligopeptide, 9.5 parts calcium β-hydroxy-β-methylbutyrate, 5.5 parts L-carnitine tartrate, 4.5 parts creatine monohydrate, 4 parts β-alanine, and 4.5 parts medium-chain glycerols. Triester, 2.8 parts curcumin-phospholipid complex prepared by water-based process, 2.8 parts tea polyphenol microcapsules, 1.8 parts vitamin D3-K2 complex, 1.8 parts food-grade mannosamine-chondroitin sulfate complex, 1 part coenzyme Q10-cyclodextrin inclusion complex, 0.5 parts glycine chelated zinc, 0.5 parts glycine chelated magnesium, 4.5 parts probiotic complex, 5.5 parts soluble dietary fiber, 2.5 parts natural sweetener complex, and 1.5 parts food-grade thickener and stabilizer.

[0073] The preparation method is basically the same as in Example 1, but it has been optimized to meet the specific needs of patients with chronic wasting diseases: In the preparation of bovine bone collagen oligopeptides, special enzymatic hydrolysis aids, including 0.2% sodium citrate and 0.1% zinc chloride, are added. These aids can enhance enzyme activity, improve enzymatic hydrolysis efficiency, and promote the generation of specific functional oligopeptides, such as the Pro-Gly-Pro sequence with immunomodulatory effects.

[0074] The hydrolyzed whey protein isolate uses highly hydrolyzed products (degree of hydrolysis >30%), contains almost no intact protein, minimizes allergenicity, and is suitable for patients with chronic diseases and compromised immune function.

[0075] The content of water-based curcumin-phospholipid complex and tea polyphenol microcapsules was increased to 2.8 parts to enhance anti-inflammatory and antioxidant effects, which can help reduce inflammatory responses and oxidative stress associated with chronic diseases.

[0076] The content of medium-chain triglycerides has been increased to 4.5 parts, providing an easily absorbed and utilized energy source, suitable for patients with impaired digestive and absorptive functions.

[0077] The content of coenzyme Q10-cyclodextrin inclusion complex was increased to 1 part, which enhances mitochondrial function and improves cellular energy metabolism, which is especially important for muscle energy supply in patients with chronic diseases.

[0078] The content of probiotic complex has been increased to 4.5 parts and soluble dietary fiber to 5.5 parts, in order to improve gut health and nutrient absorption, which is especially important for patients with chronic wasting diseases.

[0079] It contains 1.5 parts of prickly pear vitamin C complex, which significantly improves the product's antioxidant capacity, helps enhance immune function, and promotes collagen synthesis.

[0080] The composition of this embodiment is specifically designed to meet the nutritional support needs of patients with chronic wasting diseases. Through synergistic effects of enhanced anti-inflammatory and antioxidant properties, improved immune function, and improved energy metabolism and gut health, it effectively prevents muscle loss and promotes muscle recovery. Clinical applications have shown that patients with chronic wasting diseases using this composition exhibit significantly better muscle mass and function maintenance compared to conventional nutritional support.

[0081] Comparative Example 1: Lack of a multiple protein time-release system This comparative example prepared a composition lacking a multiple protein time-release system, which, by weight, consists of the following components: 75 parts hydrolyzed whey protein isolate, 8 parts calcium β-hydroxy-β-methylbutyrate, 5 parts L-carnitine tartrate, 5 parts creatine monohydrate, 4 parts β-alanine, 3 parts medium-chain triglycerides, 2 parts curcumin-phospholipid complex prepared by water-based process, 2 parts tea polyphenol microcapsules, 1 part vitamin D3-K2 complex, 1 part food-grade mannosamine-chondroitin sulfate complex, 0.5 parts coenzyme Q10-cyclodextrin inclusion complex, 0.3 parts glycine chelated zinc, 0.3 parts glycine chelated magnesium, 3 parts probiotic complex, 4 parts soluble dietary fiber, 2 parts natural sweetener complex, and 1 part food-grade thickener and stabilizer.

[0082] The main difference between this comparative example and Example 1 is that only hydrolyzed whey protein isolate was used as the protein source, and the slow-release casein micelles, plant antioxidant-treated soy protein isolate, and bovine bone collagen oligopeptides were not used, thus failing to form a multiple protein time-release system.

[0083] The preparation method is similar to that in Example 1, but the preparation steps of the corresponding components are omitted. The hydrolyzed whey protein isolate is directly mixed with other functional ingredients, homogenized under high pressure, and then freeze-dried to obtain the final product. Quality control steps are also performed to ensure that the product meets food safety standards.

[0084] Comparative Example 2: Lack of a multi-target synergistic muscle-building mechanism This comparative example prepared a composition lacking a multi-target synergistic muscle-building mechanism, which, by weight, consists of the following components: 40 parts hydrolyzed whey protein isolate, 15 parts sustained-release casein micelles, 10 parts plant antioxidant-treated soy protein isolate, 10 parts bovine bone collagen oligopeptide, 25 parts maltodextrin, 3 parts probiotic complex, 4 parts soluble dietary fiber, 2 parts natural sweetener complex, and 1 part food-grade thickener and stabilizer.

[0085] The main difference between this comparative example and Example 1 is that it lacks functional auxiliary components such as calcium β-hydroxy-β-methylbutyrate, L-carnitine tartrate, creatine monohydrate, β-alanine, medium-chain triglycerides, curcumin-phospholipid complex prepared by water-based process, tea polyphenol microcapsules, vitamin D3-K2 complex, food-grade mannosamine-chondroitin sulfate complex, coenzyme Q10-cyclodextrin inclusion complex, glycine chelated zinc, and glycine chelated magnesium, thus failing to achieve a multi-target synergistic muscle-building mechanism.

[0086] The preparation method is similar to that of Example 1, but the preparation steps of the corresponding components are omitted, and maltodextrin is used instead of these functional ingredients, while maintaining the same total weight. Quality control steps were also performed to ensure that the product meets food safety standards.

[0087] Comparative Example 3: Lack of bioavailability enhancement technologies This comparative example prepared a composition lacking bioavailability enhancement technology, which, by weight, consists of the following components: 40 parts hydrolyzed whey protein isolate, 15 parts ordinary casein, 10 parts ordinary soy protein isolate, 10 parts ordinary collagen hydrolysate, 8 parts calcium β-hydroxy-β-methylbutyrate, 5 parts L-carnitine tartrate, 5 parts creatine monohydrate, 4 parts β-alanine, 3 parts vegetable oil, 2 parts turmeric powder, 2 parts green tea extract, 1 part vitamin D3, 1 part ordinary glucosamine and chondroitin, 0.5 parts ordinary coenzyme Q10, 0.3 parts zinc sulfate, 0.3 parts magnesium oxide, 3 parts probiotic complex, 4 parts soluble dietary fiber, 2 parts natural sweetener complex, and 1 part food-grade thickener and stabilizer.

[0088] The main difference between this comparative example and Example 1 is that it uses common forms of ingredients and does not employ special bioavailability enhancement technologies, such as slow-release casein micelles, plant antioxidant-treated soy protein isolate, bovine bone collagen oligopeptides, curcumin-phospholipid complexes prepared by water-based processes, tea polyphenol microcapsules, vitamin D3-K2 complexes, coenzyme Q10-cyclodextrin inclusion complexes, glycine chelated zinc, and glycine chelated magnesium.

[0089] The preparation method is simple: the components are mixed evenly, homogenized under high pressure, and then freeze-dried to obtain the final product. Quality control procedures were also performed to ensure the product meets food safety standards.

[0090] To verify the effectiveness of the muscle-building composition of the present invention, we conducted a series of in vitro and in vivo experiments and compared the results with comparative examples.

[0091] Experiment 1: Evaluation of in vitro protein digestibility The protein digestibility of different compositions was evaluated using a simulated gastrointestinal digestive system. The experimental methods are as follows: Each composition sample (Examples 1-7 and Comparative Examples 1-3) was incubated in simulated gastric fluid (pH 2.0, containing 0.32% pepsin) for 2 hours, and then transferred to simulated intestinal fluid (pH 7.5, containing 0.1% trypsin and 0.3% bile salts) for 4 hours. The protein content before and after digestion was determined using the Bradford method, and the protein digestibility was calculated. The experimental results are shown in Table 1. Table 1. In vitro protein digestibility of different compositions sample Protein digestibility (%) Relative standard deviation (%) Example 1 85.7 2.1 Example 2 87.2 1.8 Example 3 86.5 2.2 Example 4 89.4 1.5 Example 5 82.3 2.5 Example 6 88.1 1.7 Example 7 87.8 1.9 Comparative Example 1 76.8 2.8 Comparative Example 2 83.5 2.3 Comparative Example 3 72.3 3.1 The results showed that the muscle-building compositions of the present invention (Examples 1-7) had high in vitro protein digestibility (82.3%–89.4%), significantly higher than Comparative Example 1 (76.8%) lacking a multiple protein time-release system and Comparative Example 3 (72.3%) lacking bioavailability enhancement technology. In particular, the postoperative patient-specific muscle-building composition (Example 4, the preferred embodiment of the present invention) achieved a protein digestibility as high as 89.4%, indicating that its special formulation and preparation process can maximize protein digestibility.

[0092] The high digestibility is mainly attributed to the following mechanisms: 1) The short peptide chain structure in hydrolyzed whey protein isolate facilitates the action of digestive enzymes; 2) Plant antioxidants treat the antioxidants in soy protein isolate, protecting the protein from oxidative damage and maintaining its digestibility; 3) The low molecular weight of bovine collagen oligopeptides makes them easily absorbed in the gastrointestinal tract; 4) Although the slow-release casein micelles are digested slowly in the stomach, they are gradually released in intestinal fluid, ensuring a continuous supply of amino acids. This synergistic effect of the multiple protein time-release system is key to the high digestibility of this invention.

[0093] Experiment 2: Cell Experiment The effects of different compositions on myotube diameter and myofibrillar protein expression were evaluated in a C2C12 myotube cell model. The experimental methods are as follows: C2C12 myoblasts were cultured in DMEM medium containing 10% fetal bovine serum until 70%–80% confluence, then converted to differentiation medium containing 2% horse serum to induce differentiation into myofibrillar tubes. Six days after differentiation, cells were divided into different treatment groups and treated with the extracts (final concentration 0.1 mg / mL) of Examples 1–7 and Comparative Examples 1–3 for 24 hours. Myofibrillar tube diameter was measured using phase-contrast microscopy, and myofibrillar protein expression levels were detected using Western blot. The experimental results are shown in Table 2. Table 2. Effects of different compositions on C2C12 myotube diameter and myofibrillar protein expression. sample Increase rate of myotube diameter (%) Increase in myofibrillar protein expression rate (%) Example 1 32.4 ± 2.5 41.6 ± 3.2 Example 2 29.8 ± 2.3 38.5 ± 3.1 Example 3 36.7 ± 3.1 45.2 ± 3.7 Example 4 39.5 ± 2.8 49.3 ± 3.5 Example 5 25.6 ± 2.2 33.7 ± 2.9 Example 6 38.2 ± 2.6 47.8 ± 3.4 Example 7 37.5 ± 2.7 46.4 ± 3.3 Comparative Example 1 21.3 ± 2.1 28.6 ± 2.5 Comparative Example 2 18.7 ± 1.9 25.4 ± 2.3 Comparative Example 3 15.9 ± 1.8 22.1 ± 2.0 The results showed that the muscle-building compositions of the present invention (Examples 1-7) significantly increased the diameter of C2C12 myotubes (25.6%–39.5%) and myofibrillar protein expression (33.7%–49.3%), with significantly better effects than the comparative examples. In particular, the best example (Example 4) showed an increase in myotube diameter of 39.5% and an increase in myofibrillar protein expression of 49.3%, indicating that it has the strongest promoting effect on muscle protein synthesis.

[0094] This significant effect on promoting muscle protein synthesis is mainly attributed to the following mechanisms: 1) The multiple time-release protein system provides a continuous supply of amino acids, especially components rich in branched-chain amino acids such as leucine, which can effectively activate the mTOR signaling pathway; 2) β-hydroxy-β-methylbutyrate calcium inhibits muscle protein degradation and reduces protein loss; 3) Specific amino acid sequences in bovine bone collagen oligopeptides can stimulate the secretion of IGF-1, further promoting muscle protein synthesis; 4) Curcumin-phospholipid complex and tea polyphenol microcapsules prepared by water-based processes create a microenvironment conducive to muscle growth by inhibiting inflammatory factors and oxidative stress.

[0095] Experiment 3: Animal Experiment The muscle-building effects of different compositions were evaluated using a hindlimb suspension-induced muscle atrophy model. The experimental methods are as follows: One hundred 8-week-old male SD rats were randomly divided into 10 groups: normal control group, muscle atrophy model group, treatment groups of Examples 1-7, and treatment groups of Comparative Examples 1-3, with 10 rats in each group. Except for the normal control group, the other groups were subjected to hindlimb suspension for 14 days to establish a muscle atrophy model. After model establishment, each treatment group was administered the corresponding composition by gavage (dose 2 g / kg / d) for 28 consecutive days. At the end of the experiment, the weight of the gastrocnemius muscle, grip strength, and fatigue tolerance time of each group were measured. The results are shown in Table 3. Table 3 Effects of different compositions on rats with muscular atrophy Group Gastrocnemius muscle weight recovery rate (%) Grip strength increase rate (%) Increase rate of fatigue tolerance time (%) normal control group 100.0 ± 3.2 - - Muscle atrophy model group 68.5 ± 2.8 0 0 Example 1 Group 94.2 ± 3.1 36.4 ± 2.7 42.7 ± 3.3 Example 2 group 91.8 ± 3.0 32.7 ± 2.5 38.5 ± 3.1 Example 3 Group 95.6 ± 3.2 39.8 ± 2 45.2 ± 3.5 Example 4 group 97.2 ± 3.4 43.2 ± 3.2 49.6 ± 3.8 Example 5 group 88.5 ± 2.9 30.2 ± 2.4 35.6 ± 2.9 Example 6 group 96.3 ± 3.3 41.5 ± 3.0 47.8 ± 3.6 Example 7 group 95.8 ± 3.2 40.3 ± 2.9 46.5 ± 3.5 Comparative Example 1 82.6 ± 2.7 25.8 ± 2.2 29.5 ± 2.5 Comparative Example 2 78.3 ± 2.6 22.4 ± 2.0 26.8 ± 2.3 Comparative Example 3 Groups 75.2 ± 2.5 19.7 ± 1.8 23.5 ± 2.1 The results showed that the muscle-building compositions of the present invention (Examples 1-7) significantly promoted the recovery of gastrocnemius muscle weight (88.5%–97.2%), increased grip strength (30.2%–43.2%), and improved fatigue endurance time (35.6%–49.6%) in rats with muscle atrophy, with effects significantly superior to those of the control groups. In particular, the optimal embodiment (Example 4) showed a gastrocnemius muscle weight recovery rate as high as 97.2%, close to the level of the normal control group, and increases in grip strength and fatigue endurance time of 43.2% and 49.6%, respectively, indicating that it has the strongest anti-muscle atrophy and muscle function-improving effects.

[0096] In addition, Western blot analysis was performed on the muscle protein synthesis signaling pathway in the gastrocnemius muscle tissue of each group of rats, and the results are shown in Table 4: Table 4. Effects of different compositions on muscle protein synthesis signaling pathways (fold relative to the normal control group) Group mTOR phosphorylation level p70S6K phosphorylation level 4E-BP1 phosphorylation level normal control group 1.00 ± 0.10 1.00 ± 0.08 1.00 ± 0.09 Muscle atrophy model group 0.48 ± 0.06 0.42 ± 0.05 0.55 ± 0.07 Example 1 Group 2.76 ± 0.25 3.18 ± 0.30 2.35 ± 0.22 Example 2 group 2.58 ± 0.23 2.95 ± 0.28 2.17 ± 0.20 Example 3 Group 2.94 ± 0.27 3.42 ± 0.32 2.53 ± 0.24 Example 4 group 3.35 ± 0.31 3.85 ± 0.36 2.96 ± 0.28 Example 5 group 2.32 ± 0.21 2.67 ± 0.25 1.96 ± 0.18 Example 6 group 3.15 ± 0.29 3.65 ± 0.34 2.74 ± 0.26 Example 7 group 3.08 ± 0.28 3.53 ± 0.33 2.65 ± 0.25 Comparative Example 1 1.78 ± 0.16 2.08 ± 0.19 1.48 ± 0.14 Comparative Example 2 1.56 ± 0.14 1.82 ± 0.17 1.29 ± 0.12 Comparative Example 3 Groups 1.38 ± 0.13 1.62 ± 0.15 1.17 ± 0.11 The results showed that the muscle-building compositions of the present invention (Examples 1-7) significantly activated the muscle protein synthesis signaling pathway, including the phosphorylation levels of mTOR, p70S6K, and 4E-BP1, with significantly better effects than the control groups. In particular, the best embodiment (Example 4) showed phosphorylation levels of mTOR, p70S6K, and 4E-BP1 that were 3.35 times, 3.85 times, and 2.96 times higher than those of the normal control group, indicating that it had the strongest activating effect on the muscle protein synthesis signaling pathway.

[0097] The excellent anti-muscle atrophy effect of the muscle-building composition of the present invention is mainly due to the following mechanism of action: 1. Protein synthesis promotion: The multiple time-release protein system provides a continuous supply of amino acids, especially components rich in essential and branched-chain amino acids, which activate the mTOR signaling pathway and promote protein synthesis.

[0098] 2. Inhibition of protein degradation: Calcium β-hydroxy-β-methylbutyrate reduces muscle protein degradation by inhibiting the ubiquitin-proteasome system and the autophagy-lysosome system. Studies have shown that calcium β-hydroxy-β-methylbutyrate can significantly inhibit the expression of muscle atrophy-related genes MuRF1 and MAFbx, which play key roles in muscle protein degradation.

[0099] 3. Optimized Energy Metabolism: The synergistic effect of creatine monohydrate, L-carnitine tartrate, and medium-chain triglycerides optimizes muscle energy metabolism and improves the efficiency of ATP production and utilization. Creatine monohydrate generates ATP through rephosphorylation of ADP, providing immediate energy for muscle contraction; L-carnitine promotes the transport of fatty acids into mitochondria for β-oxidation, improving fat utilization efficiency; and medium-chain triglycerides provide a rapidly absorbed energy source.

[0100] 4. Anti-inflammatory and antioxidant effects: The curcumin-phospholipid complex and tea polyphenol microcapsules prepared by water-based processes alleviated the inflammatory response and oxidative stress associated with muscle atrophy by inhibiting the NF-κB signaling pathway and scavenging free radicals. Studies have shown that chronic inflammation and oxidative stress are important factors that accelerate muscle atrophy, and the anti-inflammatory and antioxidant components in this invention can effectively alleviate these adverse factors.

[0101] 5. Skeletal muscle stem cell activation: Specific amino acid sequences in bovine bone collagen oligopeptides can stimulate the secretion of IGF-1, promote the activation, proliferation and differentiation of skeletal muscle stem cells (satellite cells), and enhance muscle regeneration.

[0102] Experiment 4: Human Clinical Trial To verify the efficacy of the muscle-building composition of this invention in humans, we conducted a small-scale clinical trial. The experimental method is as follows: Ninety healthy volunteers were recruited and divided into three groups: an elderly group (30 elderly people aged 65-75), an exercise group (30 fitness enthusiasts aged 25-35), and a rehabilitation group (30 patients in the postoperative recovery period). Each group was further randomly assigned to an experimental group and a control group. The experimental group took a corresponding special muscle-building composition daily (the elderly group took the formula of Example 2, the exercise group took the formula of Example 3, and the rehabilitation group took the formula of Example 4), with a daily dose of 30g; the control group took commercially available muscle-building products, using the dosage according to the product instructions. The trial lasted for 12 weeks, with assessments conducted every 4 weeks to measure relevant indicators. The results are shown in Tables 5, 6, and 7. Table 5. Effects of different compositions on the elderly population (rate of change after 12 weeks) index Experimental group (%) Control group (%) p-value Increase rate of skeletal muscle index (ASMI) in the limbs 8.6 ± 0.7 5.2 ± 0.5 <0.01 Grip strength increase rate 14.3 ± 1.2 8.7 ± 0.8 <0.01 6-minute walk test increase rate 12.8 ± 1.1 7.5 ± 0.7 <0.01 Increase rate of serum creatinine / cystatin C ratio 9.2 ± 0.8 5.8 ± 0.6 <0.01 Daily Living Skills Score Improvement Rate 15.6 ± 1.3 9.3 ± 0.9 <0.01

[0103] Table 6. Effects of different compositions on fitness enthusiasts (rate of change after 12 weeks) index Experimental group (%) Control group (%) p-value Fat-free weight gain rate 6.4 ± 0.6 4.1 ± 0.4 <0.01 Maximum Strength (1RM) Increase Rate 18.7 ± 1.5 11.3 ± 1.0 <0.01 Increase rate of endurance test 23.5 ± 1.9 14.8 ± 1.2 <0.01 24-hour post-exercise creatine kinase level reduction rate 31.2 ± 2.5 19.5 ± 1.6 <0.01 Improvement rate of training recovery speed 27.6 ± 2.2 16.8 ± 1.4 <0.01 Table 7. Effects of different compositions on patients in the postoperative recovery period (change rate after 12 weeks) index Experimental group (%) Control group (%) p-value Muscle mass retention rate 95.3 ± 3.2 82.7 ± 2.8 <0.01 Grip strength recovery rate 92.8 ± 3.1 79.5 ± 2.7 <0.01 Walking ability recovery rate 94.5 ± 3.2 81.2 ± 2.7 <0.01 Rate of reduction of inflammatory markers 38.6 ± 2.5 25.4 ± 1.8 <0.01 Hospital stay reduction rate 18.3 ± 1.5 10.7 ± 0.9 <0.01 The results showed that the muscle-building composition of the present invention exhibited significant muscle-building effects in the elderly, fitness enthusiasts, and patients in the postoperative recovery period, with all indicators superior to the control group, and the differences were statistically significant (p<0.01). In particular, in the postoperative recovery period, Example 4 (the best example) showed the most significant effect, with a muscle mass retention rate of up to 95.3%, a grip strength and walking ability recovery rate of 92.8% and 94.5%, respectively, an inflammatory marker reduction rate of 38.6%, and a hospital stay reduction rate of 18.3%.

[0104] These results fully demonstrate the superior effects of the muscle-building composition of the present invention, especially the differentiated formulation design for different populations, which can precisely meet specific needs. Example 4, as the preferred embodiment of the present invention, exhibits the best results in a patient group during the postoperative recovery period. Its excellent performance mainly stems from the following aspects: 1. Three-step enzymatic hydrolysis process: Bovine bone collagen oligopeptides prepared by a three-step enzymatic hydrolysis process are rich in specific amino acid sequences that promote wound healing, such as glycine-proline-hydroxyproline and proline-hydroxyproline-glycine. These sequences not only promote muscle protein synthesis but also accelerate the wound healing process.

[0105] 2. Immunomodulatory function: Whey protein isolates with moderate degree of hydrolysis retain certain immunomodulatory activities, which can enhance the immune function of postoperative patients and reduce the risk of infection.

[0106] 3. Ultra-long-term sustained-release system: Sustained-release casein micelles prepared by double-layer embedding technology achieve ultra-long-term sustained release of protein (up to 8 hours), providing postoperative patients with a continuous supply of amino acids to meet the nutritional needs of tissue repair and muscle reconstruction.

[0107] 4. Enhanced anti-inflammatory and antioxidant effects: Increased content of curcumin-phospholipid complex and tea polyphenol microcapsules enhanced anti-inflammatory and antioxidant effects, effectively reduced postoperative inflammatory response, and promoted recovery.

[0108] 5. Antimicrobial protection: The specially added antimicrobial peptide complex provides broad-spectrum antimicrobial protection, reduces the risk of postoperative infection, and creates a favorable environment for muscle recovery.

[0109] The molecular mechanism of the synergistic muscle-building effect of the muscle-building composition of the present invention is analyzed as follows: 1. Bidirectional regulation of protein synthesis and degradation balance: The composition of the present invention promotes protein synthesis by activating the mTOR signaling pathway, while inhibiting the expression of MuRF1 and MAFbx genes to reduce protein degradation, thus achieving bidirectional regulation of protein metabolism, which cannot be achieved by a single component.

[0110] 2. Synergistic activation of energy sensing pathways: Simultaneous activation of the mTOR and AMPK signaling pathways optimizes the balance between energy metabolism and protein synthesis, a balance crucial for efficient muscle growth. The mTOR signaling pathway is primarily responsible for protein synthesis, while the AMPK signaling pathway regulates energy metabolism; their synergistic activation creates a metabolic environment conducive to muscle growth.

[0111] 3. Promotion of the entire muscle differentiation process: By upregulating the expression of MyoD and Myogenin, the proliferation, differentiation, and fusion of muscle stem cells were promoted, accelerating the formation and hypertrophy of muscle fibers. MyoD mainly regulates the early differentiation of muscle stem cells, while Myogenin participates in late differentiation and myotube formation. The synergistic upregulation of the two ensures the smooth progress of the muscle differentiation process.

[0112] 4. Inhibition of the inflammation-oxidative stress axis: By reducing the expression of inflammatory factors and increasing the activity of antioxidant enzymes, a microenvironment favorable to muscle growth is created. Chronic inflammation and oxidative stress are important causes of muscle atrophy; inhibiting this axis can effectively protect muscles from damage and promote repair and growth.

[0113] 5. System optimization of bioavailability: Through various bioavailability enhancement technologies, such as curcumin-phospholipid complexes prepared by water-based processes, microencapsulation technology, and chelate formation, the absorption and utilization efficiency of active ingredients has been significantly improved, enhancing the overall effect.

[0114] This multi-target, multi-level synergistic mechanism is the key to the superiority of the muscle-building composition of this invention over the prior art, and it is also the basis for its ability to adapt to the specific needs of different groups of people.

[0115] This invention provides a multi-protein complex muscle-building composition, its preparation method, and its application. Through innovative improvements to traditional muscle-building products, it achieves the following technological breakthroughs: 1. A multi-time protein release system was established: By precisely proportioning hydrolyzed whey protein isolate (rapid absorption), plant antioxidant-treated soy protein isolate (medium-speed absorption), and slow-release casein micelles (slow release), an all-weather protein supply system was constructed to ensure continuous and stable muscle protein synthesis.

[0116] 2. A high-bioactivity bovine bone collagen oligopeptide preparation technology was developed: a unique multi-step enzymatic hydrolysis-membrane separation technology was used to prepare low molecular weight collagen oligopeptides rich in proline-hydroxyproline-glycine sequences, which significantly improved their bioactivity and absorption and utilization rate.

[0117] 3. A multi-target synergistic muscle-building mechanism was constructed: through the synergistic effects of multiple components such as β-hydroxy-β-methylbutyrate calcium inhibiting muscle protein degradation, β-alanine increasing muscle buffering capacity, L-carnitine optimizing fat metabolism, creatine increasing ATP reserves, curcumin-phospholipid complex and tea polyphenols anti-inflammatory and antioxidant effects, and vitamin D3-K2 complex regulating calcium metabolism, a multi-target regulation of protein synthesis promotion, protein degradation inhibition, energy metabolism optimization, and inflammation suppression was achieved.

[0118] 4. Several innovative technologies for enhancing bioavailability have been developed: water-based processes are used to prepare curcumin-phospholipid complexes, microencapsulation technology is used to protect tea polyphenols, cyclodextrin inclusion enhances the water solubility of coenzyme Q10, and amino acid chelation enhances mineral absorption, which significantly improves the bioavailability of active ingredients.

[0119] 5. Differentiated formulation design for different groups: Optimized component ratios and preparation processes to meet the specific needs of different groups such as the elderly, fitness enthusiasts, postoperative patients, long-term bedridden patients, and patients with chronic wasting diseases, providing more precise muscle-building solutions.

[0120] 6. A rigorous quality control system has been established: from raw material selection and production process control to finished product testing and batch management, a comprehensive quality control system has been established to ensure the safety and effectiveness of products.

[0121] Through systematic verification through in vitro experiments, cell experiments, animal experiments, and human clinical trials, it has been demonstrated that the muscle-building composition of the present invention has a significantly better muscle-building effect than the prior art. In particular, Example 4 (the best embodiment) shows excellent performance in protecting the muscles of patients after surgery, demonstrating the important application value of the present invention in the medical field.

[0122] This invention not only fills the gaps in existing muscle-building products in terms of multiple protein time-release systems, multi-target synergistic muscle-building mechanisms, and bioavailability enhancement technologies, but also provides effective solutions for various application scenarios such as preventing and improving sarcopenia, promoting muscle recovery after exercise, preventing muscle loss in postoperative and bedridden patients, and supporting the nutritional needs of patients with chronic wasting diseases. It has significant social value and broad market prospects.

Claims

1. A muscle-building composition, characterized in that... It contains the following components by weight: 30–50 parts of hydrolyzed whey protein isolate; 10–20 portions of sustained-release casein micelles; Soy protein isolate was treated with 5–15 parts of plant antioxidants; 5-15 parts bovine bone collagen oligopeptides; 5–10 parts of calcium β-hydroxy-β-methylbutyrate; 3–8 parts L-carnitine tartrate; 3-8 parts creatine monohydrate; 2–6 parts β-alanine; 1 to 5 parts medium-chain triglycerides; 1-3 parts of curcumin-phospholipid complex prepared by water-based process; 1-3 portions of tea polyphenol microcapsules; 0.5–2 parts of vitamin D3-K2 complex; 0.5–2 parts of food-grade mannosamine-chondroitin sulfate complex; 0.3–1 part coenzyme Q10-cyclodextrin inclusion complex; 0.1–0.5 parts glycine-chelated zinc; 0.1–0.5 parts glycine chelated magnesium; 2-5 parts probiotic complex; 2–6 servings of soluble dietary fiber; 1 to 3 parts of natural sweetener complex; 0.5 to 2 parts of food-grade thickener and stabilizer.

2. The muscle-building composition according to claim 1, characterized in that... The bovine bone collagen oligopeptide is rich in the proline-hydroxyproline-glycine sequence, has an average molecular weight of less than 3,000 Daltons, and is prepared by food-grade enzymatic hydrolysis-membrane separation technology.

3. The muscle-building composition according to claim 1, characterized in that... The sustained-release casein micelles are prepared by dissolving sodium caseinate in purified water, adding calcium chloride to form micelles, and then cross-linking and immobilizing them with food-grade transglutaminase.

4. The muscle-building composition according to claim 1, characterized in that... The plant antioxidant-treated soy protein isolate is prepared by mixing soy protein isolate with purified water, adding tea polyphenols and rosemary extract, stirring at 40°C for 2 hours, and then spray drying.

5. A method for preparing the muscle-building composition according to any one of claims 1-4, characterized in that... Includes the following steps: (1) Preparation of bovine bone collagen oligopeptides: Remove connective tissue from bovine bones, wash and crush them, mix them with purified water at a ratio of 1:6, and cook under high pressure at 0.8 MPa for 90 minutes; cool to 50℃, adjust pH to 7.5-8.0, add papain and trypsin for 16 hours of enzymatic hydrolysis; adjust pH to 6.5-7.0, add collagenase and protease for 12 hours of enzymatic hydrolysis; inactivate enzymes in a 95℃ water bath for 15 minutes; separate and collect the permeate through an ultrafiltration membrane with a molecular weight cutoff of 3000 Daltons; separate by chromatographic separation with food-grade ion exchange resin; freeze-dry to obtain bovine bone collagen oligopeptides; (2) Preparation of plant antioxidant-treated soy protein isolate: Soy protein isolate and purified water are mixed and stirred evenly at a ratio of 1:10; 0.5% tea polyphenols and 0.2% rosemary extract are added and stirred at 40℃ for 2 hours; spray drying is carried out at an inlet temperature of 180-200℃ and an outlet temperature of 85-95℃ to obtain the product. (3) Preparation of sustained-release casein micelles: Sodium caseinate and purified water are mixed at a ratio of 1:15, and 0.2% sodium carbonate is added and stirred to dissolve; 3% calcium chloride solution is added dropwise under stirring until the pH value is 6.7-6.9; 0.1% transglutaminase is added, and the reaction is carried out at 40℃ for 4 hours; the product is obtained by spray drying at an inlet temperature of 170-190℃ and an outlet temperature of 80-90℃. (4) Preparation of water-based curcumin-phospholipid complex: Curcumin was extracted from turmeric using supercritical carbon dioxide extraction technology; curcumin and soybean phospholipids were mixed in a ratio of 1:4, purified water was added, and nanoemulsion was prepared by high pressure homogenization at 100 MPa; the product was obtained by spray drying at an inlet temperature of 160-180℃ and an outlet temperature of 75-85℃. (5) The hydrolyzed whey protein isolate, plant antioxidant-treated soy protein isolate, slow-release casein micelles and bovine bone collagen oligopeptides were mixed to obtain a protein premix. (6) Add calcium β-hydroxy-β-methylbutyrate, L-carnitine tartrate, creatine monohydrate, β-alanine and medium-chain triglycerides to the protein premix and mix well; (7) Add curcumin-phospholipid complex, tea polyphenol microcapsules, vitamin D3-K2 complex, mannosamine-chondroitin sulfate complex, coenzyme Q10-cyclodextrin inclusion complex, glycine chelated zinc and glycine chelated magnesium prepared by water-based process respectively, and mix evenly. (8) Add microencapsulated probiotic complex and soluble dietary fiber; (9) Add the natural sweetener complex and food-grade thickener and stabilizer, and mix well; (10) Homogenized under 100-150 MPa ultra-high pressure; (11) Pre-freeze to -40°C, freeze dry for 36 hours under vacuum conditions of less than 20 Pa to obtain the final product.

6. The preparation method according to claim 5, characterized in that... The food-grade probiotic complex contains lactic acid bacteria and bifidobacteria, the soluble dietary fiber contains inulin and fructooligosaccharides, the natural sweetener complex contains mogrosides and steviol glycosides, and the food-grade thickener and stabilizer contains xanthan gum and guar gum.

7. The preparation method according to claim 5, characterized in that... The preparation method also includes quality control steps: testing for microbial indicators, heavy metal content, and pesticide residues to ensure compliance with national food safety standards; establishing a batch management system for raw materials, semi-finished products, and finished products; implementing a full-process quality control and traceability system; and retaining samples of each batch of products for regular testing.

8. The use of the muscle-building composition according to any one of claims 1-4 in the preparation of a drug or functional food for the prevention and improvement of sarcopenia.

9. The use of the muscle-building composition according to any one of claims 1-4 in the preparation of a drug or functional food for promoting muscle recovery and growth after exercise.

10. The use of the muscle-building composition according to any one of claims 1-4 in the preparation of the following pharmaceuticals or functional foods: pharmaceuticals or functional foods for the prevention of muscle loss in postoperative patients; pharmaceuticals or functional foods for the prevention of muscle atrophy in long-term bedridden patients; pharmaceuticals or functional foods for nutritional support in patients with chronic wasting diseases; and pharmaceuticals or functional foods for muscle protection in people under special circumstances.