Dehydroabietic acid locust bean gum ester as well as preparation method and application thereof
By preparing amphiphilic dehydroabscisic acid locust bean gum ester, the limitations of polysaccharide-modified substances in specific fields have been overcome, enabling their wide application in food, drug carriers, tissue engineering, and environmental protection, with good biocompatibility and degradability.
Patent Information
- Application Number
- CN202511451079.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-11
- Publication Date
- 2025-11-25
AI Technical Summary
The lack of amphiphilic polysaccharide-modified substances in existing technologies limits their application in food, drug carriers, tissue engineering, and environmental protection.
Purified locust bean gum and dehydroabsic acid chloride were used as raw materials. Pyridine was used as a catalyst, acid-binding agent and solvent. The reaction was carried out under a protective atmosphere to generate esters. Dehydroabsic acid locust bean gum esters with low degree of substitution that are insoluble in pyridine and high degree of substitution that are soluble in pyridine were obtained by centrifugation and Soxhlet extraction. The degree of substitution was optimized by combining different reaction conditions.
The prepared dehydroabscitic acid locust bean gum ester exhibits amphiphilic properties, good biocompatibility and biodegradability, and is widely used in the food industry, drug carrier, tissue engineering and environmental protection fields. It is also simple to operate, reacts rapidly and is highly efficient.
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Figure CN121005800A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of polysaccharide modification technology, specifically to a dehydroabscisic acid locust bean gum ester, its preparation method, and its application. Background Technology
[0002] Dehydroabietic acid, also known as dehydrorosin acid, is a bioactive diterpenoid phytochemical found in various pine species. It is a tricyclic diterpenoid compound with a carboxyl group (-COOH) and an aromatic ring in its molecular structure, exhibiting hydrophobicity and some polarity. It has significant applications in various fields, including pharmaceuticals, industry, food, and cosmetics. For example, in industrial applications, dehydroabietic acid and its derivatives are used as tackifiers, curing agents, and stabilizers, and are widely used in adhesives and coatings.
[0003] Robinia pseudoacacia gum (LBG), also known as sophora bean gum, is a natural polysaccharide extracted from the seeds of the black locust tree. It is mainly composed of galactose and mannose and is widely used in food, pharmaceuticals, and cosmetics. Recently, research on the modification of LBG has become a popular focus, as it shows broad application prospects in functional foods, biomaterials, and drug delivery systems. Furthermore, the abundant hydroxyl functional groups (-OH) on the galactomannan chains endow LBG with good hydrophilicity and excellent water solubility.
[0004] Natural polysaccharides and their derivatives have become a research hotspot due to their excellent biocompatibility and biodegradability, providing abundant raw material sources for the development of polysaccharide esters. Meanwhile, polysaccharide esters have significant application value in the food industry, pharmaceuticals, cosmetics, and materials science. For example, chitosan esters and alginate esters can be used as drug carriers to develop sustained-release formulations, effectively regulating drug release rates. Therefore, it is essential to continuously develop novel modified polysaccharide substances. Summary of the Invention
[0005] To address the shortcomings of the existing technology, the purpose of this invention is to provide a dehydroabscisic acid locust bean gum ester, its preparation method, and its application, thereby preparing a novel dehydroabscisic acid locust bean gum ester.
[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution.
[0007] A method for preparing dehydroabscisic acid locust bean gum ester includes the following steps: Using purified locust bean gum and dehydroabscisic acid chloride as raw materials, and pyridine as a catalyst, acid-binding agent and solvent, the reaction was carried out under a protective atmosphere to generate an ester. After the reaction was completed, the precipitate was collected for the first time after the first centrifugation to obtain the first product. The first product was extracted by Soxhlet extraction and dried to obtain dehydroabscisic acid locust bean gum ester that is insoluble in pyridine. The centrifuged liquid from the first centrifugation was added to a mixed solution containing methanol and water, and the precipitate was collected after a second centrifugation to obtain the second product. The second product was extracted by Soxhlet extraction and dried to obtain pyridine-soluble dehydroabscisic acid locust bean gum ester.
[0008] In a preferred embodiment of the present invention, the molar ratio of purified locust bean gum to dehydroabscisic acid chloride is 1:1~10.
[0009] In a preferred embodiment of the present invention, the heating reaction temperature is 30 ℃ to 120 ℃, and the heating reaction time is 2 h to 36 h.
[0010] In a preferred embodiment of the present invention, in the mixed solution containing methanol and water, the volume percentage of methanol is 40% to 80%, and the remainder is water. Preferably, the water is deionized water.
[0011] In a preferred embodiment of the present invention, the solvent for Soxhlet extraction is methanol.
[0012] In a preferred embodiment of the present invention, dehydroabscisic acid chloride is obtained by chlorinating dehydroabscisic acid with oxalyl chloride.
[0013] In a preferred embodiment of the present invention, the purified locust bean gum is obtained by alcohol precipitation of locust bean gum.
[0014] Another object of the present invention is to provide a dehydroabscisic acid locust bean gum ester prepared by any of the above preparation methods.
[0015] The chemical reaction equation for the preparation of the dehydroabscisic acid locust bean gum ester is as follows: ; Where (n) = 10~100. In this embodiment of the invention, (n) = 40 is selected.
[0016] In a preferred embodiment of the present invention, the degree of substitution of the dehydroabscitic acid locust bean gum ester is 0.001 to 2.00.
[0017] The present invention also provides an application of dehydroabscisic acid locust bean gum ester, wherein the application is as an emulsifier.
[0018] Compared with the prior art, the beneficial effects of the present invention are: 1. The dehydroabscisic acid locust bean gum ester prepared by this invention uses purified locust bean gum and dehydroabscisic acid chloride as raw materials, and pyridine as a catalyst, acid-binding agent and solvent. The reaction is carried out under a protective atmosphere by heating to generate ester. After the reaction is completed, the precipitate is collected for the first time after a first centrifugation to obtain the first product. The first product is extracted by Soxhlet extraction and dried to obtain dehydroabscisic acid locust bean gum ester that is insoluble in pyridine. The centrifuged liquid after the first centrifugation is added to a mixed solution containing methanol and water to precipitate. After a second centrifugation, the precipitate is collected for the second time to obtain the second product. The second product is extracted by Soxhlet extraction and dried to obtain dehydroabscisic acid locust bean gum ester that is soluble in pyridine. The above method can combine the hydrophilicity of locust bean gum and the hydrophobicity of dehydroabsic acid, so that dehydroabsic acid locust bean gum ester exhibits amphiphilic properties, namely hydrophilic and lipophilic. It can be used as an emulsifier and has a wide range of applications in the food industry, drug carriers, tissue engineering, biosensing, environmental protection and other fields. Moreover, both locust bean gum and dehydroabsic acid are natural products with wide sources and excellent biocompatibility and biodegradability.
[0019] 2. The preparation method of this invention has significant advantages such as simple operation, rapid reaction, and high efficiency. Furthermore, according to the verification of this invention, pyridine is crucial for improving the yield of dehydroabscisic acid locust bean gum.
[0020] 3. This invention also provides guidance for the synthesis of polysaccharide ester compounds with controllable substitution degree by adjusting the reaction temperature within the range of 30 ℃ to 120 ℃, the reaction time within the range of 2 h to 36 h, and the molar ratio of purified locust bean gum to dehydroabscisic acid chloride within the range of 1:1 to 10. Attached Figure Description
[0021] Figure 1 FT-IR spectra of DA-Cl, LBG, DLE, and DLE at different raw material molar ratios; Among them, (a) is the FT-IR spectrum of DA-Cl, LBG and DLE, (b) is the FT-IR spectrum of the first product and (c) is the FT-IR spectrum of the second product.
[0022] Figure 2 Here are the H-NMR spectra of DA-Cl, LBG, and DLE.
[0023] Figure 3 Raman plots for LBG, DLE, and DLE at different feed molar ratios; Among them, (a) is the Raman diagram of LBG and DLE, (b) is the Raman diagram of the first product, and (c) is the Raman diagram of the second product.
[0024] Figure 4 This is a GPC diagram of DLE under different raw material molar ratios according to the present invention.
[0025] Figure 5 The contact angle diagrams of DLE under different raw material molar ratios according to the present invention are shown. (a) is the contact angle diagram of the first product, and (b) is the contact angle diagram of the second product.
[0026] Figure 6 This is a diagram showing the degree of substitution of DLE under different raw material molar ratios according to the present invention; Among them, (a) is the substitution degree diagram of the first product, and (b) is the substitution degree diagram of the second product; Figure 7 This is a diagram showing the emulsification of rapeseed oil by DLE under different degrees of substitution and different water-oil ratios according to the present invention. Among them, (a) is a graph showing the emulsification at different times when the DS of the PI in Example 2 is 0.26, and (b) is a graph showing the emulsification at different times when the DS of the PI in Example 4 is 1.01. Detailed Implementation
[0027] The following detailed description, in conjunction with embodiments of the present invention and accompanying drawings, provides a clear and complete illustration of the technical solutions in these embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.
[0028] It should be noted that all technical terms used in this invention are for the purpose of describing specific embodiments only and are not intended to limit the scope of protection of this invention. Unless otherwise specified, all raw materials, reagents, instruments and equipment used in the following embodiments of this invention can be purchased from the market or prepared by existing methods.
[0029] The inventive concept of this invention is as follows: To develop new polysaccharide-modified substances, this invention purifies purchased locust bean gum (purchased from Aladdin, catalog number L118711) to obtain purified locust bean gum. Using purified locust bean gum and dehydroabsic acid chloride as raw materials, pyridine is used simultaneously as a catalyst, acid-binding agent, and solvent. When pyridine acts as an acid-binding agent, it primarily absorbs the hydrogen chloride produced in the reaction. The locust bean gum, dehydroabsic acid chloride, and pyridine are mixed to form a heterogeneous system. This heterogeneous system is heated under a protective atmosphere, causing the hydroxyl groups of the locust bean gum and the acyl chloride of the dehydroabsic acid chloride to react and form an ester. After the reaction, a first centrifugation is performed to obtain the first product, which is a low-substituted product. After Soxhlet extraction and drying, low-substituted dehydroabsic acid locust bean gum ester is obtained. The low-substituted dehydroabsic acid locust bean gum ester refers to the product that is insoluble in pyridine.
[0030] The centrifuged liquid from the first centrifugation was added to a mixed solution containing methanol and water, with methanol as the diluent and water as the precipitant. After precipitation, a second centrifugation was performed to obtain the second product. After Soxhlet extraction and drying, highly substituted dehydroabietic locust bean gum ester was obtained. The highly substituted dehydroabietic locust bean gum ester refers to the product soluble in pyridine.
[0031] The dehydroabscitic acid locust bean gum ester obtained by this invention is amphiphilic, that is, hydrophilic and hydrophobic, and can be applied in the fields of food industry, drug carrier, tissue engineering, biosensing, and environmental protection. Moreover, the raw materials locust bean gum and dehydroabscitic acid are both natural sources, and have good biocompatibility and safety.
[0032] To investigate the effect of different raw material molar ratios on dehydroabscisic acid locust bean gum ester, this invention includes Examples 1 to 5.
[0033] Example 1 A method for preparing dehydroabscisic acid locust bean gum ester includes the following steps: (1) Purification of locust bean gum: Take 10 g of locust bean gum and add it to 1400 mL of water. Stir the mixture at 80 °C and centrifuge at 3500 rpm for 20 min to remove the precipitate. Add anhydrous ethanol to the supernatant for precipitation. The volume ratio of supernatant to anhydrous ethanol is 1:1. Filter with rapid qualitative filter paper. Wash the precipitate three times with acetone and anhydrous ether respectively. Then freeze-dry and grind it into powder to obtain purified locust bean gum for later use.
[0034] (2) Obtaining dehydroabietic acid chloride by oxalyl chloride: 5 g of dehydroabietic acid was dissolved in 10 mL of dichloromethane, filtered through rapid qualitative filter paper, and 3 mL of oxalyl chloride was added under ice bath conditions. The reaction was carried out at 30 °C for 6 h. After the reaction was completed, the solution was distilled under reduced pressure at 70 °C for 2 h, and the tail gas was absorbed with 10% sodium hydroxide solution to obtain a golden yellow viscous dehydroabietic acid chloride.
[0035] The above process uses oxalyl chloride as the acylating agent and dichloromethane as the solvent. After the reaction is completed, the dichloromethane and oxalyl chloride in the reaction solution are distilled off to obtain a golden yellow viscous dehydroabicyl chloride.
[0036] (3) Dissolve 5.91 g of dehydroabscisic acid chloride (DA-Cl) and 0.50 g of purified locust bean gum (LBG) in 20 mL of pyridine, and heat at 90 °C under a nitrogen atmosphere. Figure 1The reaction was carried out at the indicated temperature for 10 h. After the first centrifugation, the precipitate was collected for the first time to obtain the first product, which was a low-substituted product. The first centrifugation was performed at 3500 rpm for 20 min at room temperature. In this step, the molar ratio of purified locust bean gum to dehydroabscisic acid chloride was 1:6.
[0037] The first product was subjected to Soxhlet extraction in methanol and then dried at 80°C for 12 h to obtain dehydroabscisic acid locust bean gum ester with low degree of substitution, denoted as PI.
[0038] (4) A mixed solution of methanol and water at a volume ratio of 80:20 was used as a precipitant to precipitate the centrifuged liquid after the first centrifugation. A second centrifugation was then performed at 3500 rpm at room temperature for 20 min. The precipitate collected in the second centrifugation was the second product. The second product was a product with a low degree of substitution.
[0039] The second product was subjected to Soxhlet extraction in methanol and then dried at 80°C for 12 h to obtain highly substituted dehydroabscisic acid locust bean gum ester, denoted as PII.
[0040] Example 2 A method for preparing dehydroabscisic acid locust bean gum ester differs from that in Example 1 in that: The molar ratio of LBG to DA-Cl is 1:4.
[0041] Example 3 A method for preparing dehydroabscisic acid locust bean gum ester differs from that in Example 1 in that: The molar ratio of LBG to DA-Cl is 1:5.
[0042] Example 4 A method for preparing dehydroabscisic acid locust bean gum ester differs from that in Example 1 in that: The molar ratio of LBG to DA-Cl is 1:7.
[0043] Example 5 A method for preparing dehydroabscisic acid locust bean gum ester differs from that in Example 1 in that: The molar ratio of LBG to DA-Cl is 1:8.
[0044] To investigate the effect of different temperatures on dehydroabscisic acid locust bean gum ester, Examples 6 to 8 were set up in this invention.
[0045] Example 6 A method for preparing dehydroabscisic acid locust bean gum ester differs from that in Example 1 in that: 5.91 g of DA-Cl and 0.50 g of LBG were dissolved in 20 mL of pyridine and reacted at 60 °C under a nitrogen atmosphere for 10 h.
[0046] Example 7 A method for preparing dehydroabscisic acid locust bean gum ester differs from that in Example 1 in that: 5.91 g of DA-Cl and 0.50 g of LBG were dissolved in 20 mL of pyridine and reacted at 30 °C under a nitrogen atmosphere for 10 h.
[0047] Example 8 A method for preparing dehydroabscisic acid locust bean gum ester differs from that in Example 1 in that: 5.91 g of DA-Cl and 0.50 g of LBG were dissolved in 20 mL of pyridine and reacted at 120 °C under a nitrogen atmosphere for 10 h.
[0048] To investigate the effect of different reaction times on dehydroabscisic acid locust bean gum ester, Examples 9 to 10 were set up in this invention.
[0049] Example 9 A method for preparing dehydroabscisic acid locust bean gum ester differs from that in Example 1 in that: 5.91 g of DA-Cl and 0.50 g of LBG were dissolved in 20 mL of pyridine and reacted at 90 °C under a nitrogen atmosphere for 2 h.
[0050] Example 10 A method for preparing dehydroabscisic acid locust bean gum ester differs from that in Example 1 in that: 5.91 g of DA-Cl and 0.50 g of LBG were dissolved in 20 mL of pyridine and reacted at 90 °C under a nitrogen atmosphere for 36 h.
[0051] To investigate the effects of multiple factors on dehydroabscisic acid locust bean gum ester, Examples 11 and 12 were set up in this invention.
[0052] Example 11 A method for preparing dehydroabscisic acid locust bean gum ester differs from that in Example 1 in that: 2.95 g of DA-Cl and 0.50 g of LBG were dissolved in 20 mL of pyridine and reacted at 60 °C under a nitrogen atmosphere for 12 h.
[0053] Example 12 A method for preparing dehydroabscisic acid locust bean gum ester includes the following steps: 8.86 g of dehydroabscisic acid chloride and 0.50 g of purified locust bean gum were dissolved in 20 mL of pyridine and reacted at 90 °C under a nitrogen atmosphere for 10 h.
[0054] Results Analysis Methods for calculating the degree of substitution: The product was subjected to C, H, N, and S elemental analysis using an elemental analyzer. The degree of substitution of the acyl chloride group was calculated based on the carbon content using the following formula: in, DS Indicates the degree of substitution of the acyl chloride group in dehydroabscisic acid locust bean gum ester. C % indicates the mass percentage of elemental carbon. M AGU Indicates the molecular weight of dehydrated glucose units, M Indicates the molecular weight of the acyl chloride substituent and N This indicates the number of carbon atoms in the acyl chloride.
[0055] Figure 1 FT-IR spectra of DA-Cl, LBG, DLE, and PI and PII prepared in different Examples 1 to 5 (different raw material molar ratios) are shown in the figures. The 3352 cm⁻¹ value is also visible in the figures. -1 The OH absorption peak of the ester is at 2923 cm⁻¹. -1 The absorption peaks for -CH2 and -CH3 are located at 1716 cm⁻¹. -1 The characteristic absorption peak at -C=O is 1240 cm⁻¹. -1 The absorption peaks on the left and right are attributed to the symmetric and asymmetric stretching vibrations of -CH2 and -CH3, 10¹⁸ cm⁻¹. -1 The absorption peaks around the left and right are attributed to the asymmetric stretching vibration of -COC, indicating successful DLE synthesis. It should be noted that since the peak patterns of PI and PII are consistent, [the following is a separate point:] ... Figure 1 In (a), DLE is used to represent the entire document. Figure 1 (a) is mainly to show the peak changes observed in DLE compared to DA-Cl and LBG feedstocks. Similarly, Figure 2 as well as Figure 3 In (a), since the peak patterns of PI and PII are consistent, DLE is also used to represent PI and PII.
[0056] Figure 2 The 1H-NMR spectra of DA-Cl, LBG, and DLE are shown. The figures reveal that LBG exhibits a signal in the δ 3.0 ppm–5.5 ppm range, a typical characteristic region for polysaccharides. Specifically, δ 3.0 ppm–4.5 ppm represents other hydrogen atoms on the sugar ring (H2–H6); δ 4.6 ppm represents the anomeric hydrogen atom of galactose (H1); and δ 5.0 ppm represents the anomeric hydrogen atom of mannose (H1). Comparison of the 1H spectra reveals that the LBG 1H spectrum shows a characteristic peak at δ 3.0 ppm–5.5 ppm, building upon the DA-Cl 1H spectrum, indicating successful DLE synthesis.
[0057] Figure 3 Raman chromatograms of LBG, DLE, and PI and PII prepared in different Examples 1 to 5 are shown in the figure. The figure shows that 1610 cm⁻¹ is the maximum effective value for PI. -1 The new characteristic peak is located at 1450 cm⁻¹, representing the stretching vibration of the conjugated double bond C=C. -1 The characteristic peak at 710 cm⁻¹ represents the asymmetric bending vibration of the CH bond. -1 The characteristic peak at that location corresponds to the bending vibration of C-COO, indicating that the DLE synthesis was successful.
[0058] Figure 4 The GPC diagrams of DLE prepared in Examples 3 and 4 of this invention are shown. It can be seen from the diagrams that the molecular weight of DLE is about 18500 g / mol. Based on the molecular weight, it can be deduced that the molecular structure of DLE consists of 40 mannose chains, indicating that DLE was successfully synthesized.
[0059] Figure 5 The contact angle diagrams of PI and PII prepared in Examples 1 to 5 of this invention are shown. It can be seen from the diagrams that the contact angles of PI and PII are both around 90°, indicating that DLE is amphiphilic.
[0060] Figure 6 The figure shows the degree of substitution of PI and PII prepared in Examples 1 to 5 of this invention. It can be seen from the figure that PⅠ and PⅡ first increase and then decrease with the increase of molar ratio. The degree of substitution of 1:7 in PⅡ is 1.41. The results show that DLE was successfully synthesized and the degree of substitution is controllable.
[0061] Control group 1 A method for preparing dehydroabscisic acid locust bean gum ester differs from that in Example 1 in that: The reagent was changed from "20 mL pyridine" to "30 mL 1,4-dioxane" to investigate the effect of the reagent on the yield of dehydroabscisic acid locust bean gum.
[0062] The yield obtained in control group 1 was not as high as that obtained when pyridine was used as a solvent. This is because the dielectric constant of 1,4-dioxane is low, which is not conducive to the polar activation of acyl chloride.
[0063] The application experiment of dehydroabscisic acid locust bean gum ester as an emulsifier is as follows: The PI (DS=0.26) from Example 2 was added to a 5 mL glass bottle, followed by a phosphate buffer solution with pH 7. The mixture was stirred and dispersed at room temperature. Rapeseed oil was then added to a 0.5 wt% suspension of dehydroabscisic acid locust bean gum. The dehydroabscisic acid locust bean gum and different oil phases were mixed at a volume ratio of 3:7 or 7:3, and homogenized at 15000 rpm for 2 min to obtain a stable emulsion. The system was allowed to stand, and the emulsification status over time was observed. Additionally, following the same method, the PI (DS=1.01) from Example 4 was also used in an emulsifier application experiment. The results are shown below. Figure 7 .
[0064] from Figure 7 The results show that when rapeseed oil is used as the oil phase, the oil-water ratio of 3:7 with different degrees of substitution all show stratification after 5 minutes, while the emulsion with an oil-water ratio of 7:3 only shows stratification after 1 hour, indicating a longer stability time. This suggests that an oil-water ratio of 7:3 is the optimal oil-water ratio.
[0065] It should be noted that when numerical ranges are involved in this invention, it should be understood that both endpoints of each numerical range and any value between the two endpoints can be selected. Since the steps and methods used are the same as in the embodiments, preferred embodiments are described here to avoid redundancy. Although preferred embodiments of the invention have been described, those skilled in the art, once they understand the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of this invention.
[0066] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.
Claims
1. A method for preparing dehydroabscisic acid locust bean gum ester, characterized in that, Includes the following steps: Using purified locust bean gum and dehydroabscisic acid chloride as raw materials, and pyridine as a catalyst, acid-binding agent and solvent, the reaction was carried out under a protective atmosphere and heated to generate an ester. After the reaction was completed, the precipitate was collected for the first time after the first centrifugation to obtain the first product. The first product was extracted by Soxhlet and dried to obtain dehydroabscisic acid locust bean gum ester, which is insoluble in pyridine; The liquid obtained after the first centrifugation was added to a mixed solution containing methanol and water, and the precipitate was collected after a second centrifugation to obtain the second product. The second product was extracted using a Soxhlet extract and dried to obtain pyridine-soluble dehydroabscisic acid locust bean gum ester.
2. The method for preparing dehydroabscisic acid locust bean gum ester according to claim 1, characterized in that, The molar ratio of purified locust bean gum to dehydroabscisic acid chloride is 1:1~10.
3. The method for preparing dehydroabscisic acid locust bean gum ester according to claim 1, characterized in that, The heating reaction temperature is 30 ℃~120 ℃, and the heating reaction time is 2 h~36 h.
4. The method for preparing dehydroabscisic acid locust bean gum ester according to claim 1, characterized in that, In a mixed solution containing methanol and water, the volume percentage of methanol is 40% to 80%.
5. The method for preparing dehydroabscisic acid locust bean gum ester according to claim 1, characterized in that, The solvent used for Soxhlet extraction is methanol.
6. The method for preparing dehydroabscisic acid locust bean gum ester according to claim 1, characterized in that, Dehydroabicyl chloride is obtained by chlorinating dehydroabicyl chloride with oxalyl chloride.
7. The method for preparing dehydroabscisic acid locust bean gum ester according to claim 1, characterized in that, The purified locust bean gum was obtained by alcohol precipitation of locust bean gum.
8. A dehydroabscisic acid locust bean gum ester prepared by the preparation method according to any one of claims 1 to 7.
9. The dehydroabscisic acid locust bean gum ester according to claim 8, characterized in that, The degree of substitution of the dehydroabscitic acid locust bean gum ester is 0.001~2.
00.
10. The application of the dehydroabscisic acid locust bean gum ester according to claim 8, characterized in that, The application uses dehydroabscisic acid locust bean gum ester as an emulsifier.