Preparation method and application of repairing and soothing composition

Abstract

The invention discloses a preparation method and application of a repairing and soothing composition. Aiming at the dissolvability difference of different components in the three extracts, a step-by-step combined extraction process is adopted for extraction, that is, stephania tetrandra is subjected to composite enzymolysis and then subjected to microwave-assisted extraction, citrus peel is subjected to low-temperature fermentation and then subjected to subcritical water extraction, and pogostemon cablin leaves are subjected to supercritical CO2 extraction and then subjected to ethanol gradient elution. After the three extracts extracted by the process are compounded, the composition has excellent skin barrier repairing, moisturizing and skin relieving effects, is safe and non-irritant, and can be used for uncomfortable skin after medical beauty and season change.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a method for preparing a repairing and soothing composition and its application. Background Technology

[0002] As people's living standards improve and their awareness of skincare increases, the demand for skincare products that repair the skin barrier, moisturize, and soothe the skin is growing. This is especially true for skin that has undergone cosmetic procedures or is prone to discomfort during seasonal changes, which require effective repair to restore the skin barrier function.

[0003] In the existing technology, there are various compositions for skin repair, many of which contain plant extracts. Common plant raw materials include Stephania tetrandra, citrus peel, and patchouli leaves, whose extracts possess certain bioactivity. However, current extraction processes for these extracts mostly employ conventional solvent extraction methods, which struggle to fully extract the active ingredients and fail to fully realize the synergistic effects between different extracts, resulting in less than ideal effects in repairing the skin barrier, moisturizing, and soothing the skin.

[0004] CN111870568B discloses an anti-allergic and antipruritic plant composition, characterized in that it contains the following components obtained from raw materials in parts by weight: 1-8 parts of Stephania tetrandra, 0-6 parts of Ilex chinensis, 1-6 parts of Scutellaria baicalensis, 1-6 parts of Polygonum cuspidatum, 1-6 parts of Glycyrrhiza glabra, 0.5-6 parts of green tea, 0-6 parts of Smilax china, 0-6 parts of Melaleuca alternifolia, 0.5-6 parts of Matricaria champaca, 0.5-6 parts of Centella asiatica, and 0.5-6 parts of Rosemary; the bioactive substances of the raw materials are extracted by mixing the powders of the raw materials, including: grinding and mixing Stephania tetrandra, optional Ilex chinensis, Scutellaria baicalensis, Polygonum cuspidatum, and Glycyrrhiza glabra, and then extracting them with 40-80% ethanol; grinding and mixing green tea, optional Smilax china, optional Melaleuca alternifolia, Matricaria champaca, Centella asiatica, and Rosemary, and then extracting their volatile oils. This composition contains a variety of plant raw materials, the synergistic effect between the components is unclear, and it is extracted using a 40-80% ethanol maceration process. It does not have the function of repairing the skin barrier.

[0005] CN118649114A discloses an olive oil soothing complex with synergistic soothing effects, its preparation method, and its application. The olive oil soothing complex comprises: 5-10 parts olive leaf extract powder, 45-55 parts citrus fruit extract, 25-45 parts polyol, and 0-20 parts water. It has good soothing effects and can be used in the preparation of products for sensitive skin repair. The preparation method of the citrus fruit extract involves water extraction of citrus peel, resulting in a low yield of effective components and lacking the function of repairing the skin barrier.

[0006] Therefore, an optimized extraction process was developed for extracts of three plant materials: Stephania tetrandra, citrus peel, and patchouli leaves, to improve the efficacy of active ingredients and enhance the synergistic effect between the extracts. This resulted in a safe and non-irritating composition with excellent skin barrier repair, moisturizing, and soothing effects, suitable for various types of damaged skin, such as those after cosmetic procedures or seasonal sensitivity. Summary of the Invention

[0007] The purpose of this invention is to overcome the shortcomings of the prior art and provide a method for preparing a repairing and soothing composition and its application.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0009] In a first aspect, the present invention provides a method for preparing a repairing and soothing composition, comprising the following steps:

[0010] (1) Preparation of Stephania tetrandra extract: Take Stephania tetrandra powder and deionized water at a mass ratio of 1:(8-12), mix and stir evenly, add compound enzyme solution, and enzymatically hydrolyze for 1-2 hours at 35-40℃ and pH 4.5-5.0. After enzymatic hydrolysis, microwave-assisted extraction is performed with a microwave power of 240-360W and an extraction time of 20-25 minutes. After extraction, filter, concentrate and dry to obtain Stephania tetrandra extract.

[0011] (2) Preparation of citrus peel extract: Citrus peel powder and deionized water were mixed and stirred evenly at a mass ratio of 1:(6-10). Lactobacillus plantarum was inoculated and fermented at 30-35℃ for 8-12 hours. After fermentation, the fermentation mixture was transferred to an extraction vessel. Deionized water was added at a mass ratio of citrus peel powder to deionized water of 1:(10-20). Subcritical water extraction was then carried out at an extraction temperature of 120-130℃ and a pressure of 3-4 MPa. After extraction, the mixture was filtered, concentrated and dried to obtain citrus peel extract.

[0012] (3) Preparation of patchouli leaf extract: Patchouli leaf raw material was crushed and added to an extraction vessel. A 65-75% ethanol aqueous solution was added at a rate of 0.05-0.15 L / h as an entrainer for supercritical CO2 extraction to obtain volatile oil. The residue after extraction was refluxed with a 50%-70% ethanol aqueous solution at 60-70℃, filtered, concentrated and dried to obtain non-volatile component extract. The volatile oil and non-volatile component extract were mixed to obtain patchouli leaf extract.

[0013] (4) Mix the Stephania tetrandra extract obtained in step (1), the citrus peel extract obtained in step (2), and the patchouli leaf extract obtained in step (3) at a mass ratio of 1:(0.5-1.5):(0.5-1.5) to obtain a repair and soothing composition.

[0014] Preferably, in step (1), the complex enzyme is xylanase and acidic protease, the mass ratio of xylanase to acidic protease is 1:(0.5-1), and the amount of the complex enzyme solution is 0.5-1 wt% of the mass of Stephania tetrandra powder.

[0015] Preferably, in step (2), the inoculation amount of *Lactobacillus plantarum* is 0.4-0.8 wt% of the citrus peel powder.

[0016] Preferably, in step (3), the supercritical CO2 extraction pressure is 25-30 MPa, the extraction temperature is 40-50℃, and the extraction time is 1-1.5 h.

[0017] Preferably, in step (4), the mass ratio of the Stephania tetrandra extract, the citrus peel extract, and the patchouli leaf extract is 1:1:1.

[0018] Secondly, the present invention provides a repairing and soothing composition prepared by the above method.

[0019] Thirdly, the present invention provides the application of the above-mentioned repairing and soothing composition in the preparation of skin barrier repairing skin care products, wherein the amount of the repairing and soothing composition in the skin care product is 0.5-5 wt%, and the skin care product includes any one of cream, lotion, and serum.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0021] (1) A step-by-step combined extraction process was adopted, and extraction methods were designed for the characteristics of Stephania tetrandra, citrus peel, and patchouli leaves. The combination of complex enzymatic hydrolysis (xylanase and acidic protease) of Stephania tetrandra and microwave-assisted extraction improved the yield of active ingredients; the combination of low-temperature fermentation (Lactobacillus plantarum) of citrus peel and subcritical water extraction decomposed allergenic substances and optimized the dissolution of components; the combination of supercritical CO2 extraction and ethanol gradient elution of patchouli leaves achieved the effective extraction of volatile oils and non-volatile components.

[0022] (2) When the three extracts—Stephania tetrandra extract, Citrus peel extract, and Patchouli leaf extract—are combined in a specific ratio, they exhibit synergistic effects in repairing the skin barrier, moisturizing, and soothing the skin, and are safe and non-irritating. The anti-inflammatory properties of Stephania tetrandra extract, the stratum corneum repair-promoting properties of Citrus peel extract, and the microecological regulation properties of Patchouli leaf extract form a multi-dimensional synergistic mechanism, which is more effective than mixed extracts produced by traditional solvent extraction processes.

[0023] (3) Through innovative processes, it effectively removes irritating impurities. Skin patch tests have proven that it is non-irritating and suitable for various types of damaged skin, such as those after cosmetic procedures or those experiencing seasonal sensitivity. It can be applied to a variety of skincare products, including creams, lotions, and serums, to meet the needs of different groups, and has broad market application prospects. Detailed Implementation

[0024] To better illustrate the purpose, technical solution, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Unless otherwise specified, the materials and reagents used in the embodiments are commercially available.

[0025] The microbial sources for this invention are: xylanase (enzyme activity > 100,000 U / g) and acidic protease (enzyme activity 50,000 U / g), both purchased from Shanghai Yuanye Biotechnology Co., Ltd. *Lactobacillus plantarum*: accession number CICC20242, deposited at the China Industrial Microbial Culture Collection Center.

[0026] Example 1

[0027] A method for preparing a repairing and soothing composition includes the following steps:

[0028] (1) Preparation of Stephania tetrandra extract: Stephania tetrandra powder and deionized water were mixed and stirred evenly at a mass ratio of 1:8. A compound enzyme solution (the compound enzyme is xylanase and acidic protease at a mass ratio of 1:0.5, and the amount of compound enzyme solution is 0.5wt% of the mass of Stephania tetrandra powder) was added. Enzymatic hydrolysis was carried out at 35℃ and pH 4.5 for 2 hours. After the enzymatic hydrolysis was completed, microwave-assisted extraction was carried out. The microwave power was 240W and the extraction time was 20min. After extraction, the mixture was filtered, concentrated and dried to obtain Stephania tetrandra extract.

[0029] (2) Preparation of citrus peel extract: Citrus peel powder and deionized water were mixed and stirred evenly at a mass ratio of 1:6. Lactobacillus plantarum was inoculated at an inoculation amount of 0.4 wt% of the citrus peel powder mass. Fermentation was carried out at 30℃ for 12 h. After fermentation, the fermentation mixture was transferred to an extraction vessel. Deionized water was added at a mass ratio of citrus peel powder to deionized water of 1:10. Subcritical water extraction was then carried out at an extraction temperature of 120℃ and a pressure of 3 MPa. After extraction, the mixture was filtered, concentrated and dried to obtain citrus peel extract.

[0030] (3) Preparation of patchouli leaf extract: Patchouli leaf raw material was crushed and added to an extraction vessel. A 65% ethanol aqueous solution was added at a rate of 0.05 L / h as an entrainer for supercritical CO2 extraction. The extraction pressure was 25 MPa, the temperature was 40℃, and the extraction was carried out for 1.5 h to obtain volatile oil. The residue after extraction was refluxed with a 50% ethanol aqueous solution at 60℃, filtered, concentrated, and dried to obtain non-volatile component extract. The volatile oil and non-volatile component extract were mixed to obtain patchouli leaf extract.

[0031] (4) Mix the Stephania tetrandra extract obtained in step (1), the citrus peel extract obtained in step (2), and the patchouli leaf extract obtained in step (3) at a mass ratio of 1:0.5:0.5 to obtain a repair and soothing composition.

[0032] Example 2

[0033] A method for preparing a repairing and soothing composition includes the following steps:

[0034] (1) Preparation of Stephania tetrandra extract: Stephania tetrandra powder and deionized water were mixed and stirred evenly at a mass ratio of 1:10. A compound enzyme solution (the compound enzyme is xylanase and acidic protease, with a mass ratio of 1:0.8, and the amount of compound enzyme solution is 0.8wt% of the mass of Stephania tetrandra powder) was added. Enzymatic hydrolysis was carried out at 37℃ and pH 4.7 for 1.5h. After the enzymatic hydrolysis was completed, microwave-assisted extraction was carried out. The microwave power was 300W and the extraction time was 22min. After extraction, the mixture was filtered, concentrated and dried to obtain Stephania tetrandra extract.

[0035] (2) Preparation of citrus peel extract: Citrus peel powder and deionized water were mixed and stirred evenly at a mass ratio of 1:8. Lactobacillus plantarum was inoculated at an inoculation amount of 0.6 wt% of the citrus peel powder mass. Fermentation was carried out at 33℃ for 10 h. After fermentation, the fermentation mixture was transferred to an extraction vessel. Deionized water was added at a mass ratio of citrus peel powder to deionized water of 1:15. Subcritical water extraction was then carried out at an extraction temperature of 125℃ and a pressure of 3.5 MPa. After extraction, the mixture was filtered, concentrated and dried to obtain citrus peel extract.

[0036] (3) Preparation of patchouli leaf extract: Patchouli leaf raw material was crushed and added to an extraction vessel. A 70% ethanol aqueous solution was added at a rate of 0.1 L / h as an entrainer for supercritical CO2 extraction. The extraction pressure was 27 MPa, the temperature was 45℃, and the extraction time was 1.2 h to obtain volatile oil. The residue after extraction was refluxed with a 60% ethanol aqueous solution at 65℃, filtered, concentrated, and dried to obtain non-volatile component extract. The volatile oil and non-volatile component extract were mixed to obtain patchouli leaf extract.

[0037] (4) Mix the Stephania tetrandra extract obtained in step (1), the Citrus peel extract obtained in step (2), and the Patchouli leaf extract obtained in step (3) in a mass ratio of 1:1:1 to obtain a repair and soothing composition.

[0038] Example 3

[0039] A method for preparing a repairing and soothing composition includes the following steps:

[0040] (1) Preparation of Stephania tetrandra extract: Stephania tetrandra powder and deionized water were mixed and stirred evenly at a mass ratio of 1:12. A compound enzyme solution (the compound enzyme is xylanase and acidic protease at a mass ratio of 1:1, and the amount of compound enzyme solution is 1.0 wt% of the mass of Stephania tetrandra powder) was added. Enzymatic hydrolysis was carried out at 40℃ and pH 5.0 for 1 h. After the enzymatic hydrolysis was completed, microwave-assisted extraction was carried out. The microwave power was 360W and the extraction time was 25 min. After extraction, the mixture was filtered, concentrated and dried to obtain Stephania tetrandra extract.

[0041] (2) Preparation of citrus peel extract: Citrus peel powder and deionized water were mixed and stirred evenly at a mass ratio of 1:10. Lactobacillus plantarum was inoculated at an inoculation amount of 0.8 wt% of the citrus peel powder mass. Fermentation was carried out at 35℃ for 8 hours. After fermentation, the fermentation mixture was transferred to an extraction vessel. Deionized water was added at a mass ratio of citrus peel powder to deionized water of 1:20. Subcritical water extraction was then carried out at an extraction temperature of 130℃ and a pressure of 4 MPa. After extraction, the mixture was filtered, concentrated, and dried to obtain citrus peel extract.

[0042] (3) Preparation of patchouli leaf extract: Patchouli leaf raw material was crushed and added to an extraction vessel. A 75% ethanol aqueous solution was added at a rate of 0.15 L / h as an entrainer for supercritical CO2 extraction. The extraction pressure was 30 MPa, the temperature was 50 °C, and the extraction was carried out for 1 h to obtain volatile oil. The residue after extraction was refluxed with a 70% ethanol aqueous solution at 70 °C. The residue was filtered, concentrated, and dried to obtain non-volatile component extract. The volatile oil and non-volatile component extract were mixed to obtain patchouli leaf extract.

[0043] (4) Mix the Stephania tetrandra extract obtained in step (1), the Citrus peel extract obtained in step (2), and the Patchouli leaf extract obtained in step (3) in a mass ratio of 1:1.5:1.5 to obtain a repair and soothing composition.

[0044] Comparative Example 1

[0045] A method for preparing a repairing and soothing composition differs from Example 2 in that it contains only Stephania tetrandra extract, the preparation method of Stephania tetrandra extract is the same as in Example 2, and the mass of citrus peel extract and patchouli leaf extract in the composition is replaced by an equal amount of Stephania tetrandra extract.

[0046] Comparative Example 2

[0047] A method for preparing a repairing and soothing composition differs from Example 2 in that it contains only citrus peel extract, the preparation method of the citrus peel extract is the same as in Example 2, and the mass of Stephania tetrandra extract and patchouli leaf extract in the composition is replaced by an equal amount of citrus peel extract.

[0048] Comparative Example 3

[0049] A method for preparing a repairing and soothing composition differs from Example 2 in that it contains only patchouli leaf extract, the preparation method of patchouli leaf extract is the same as in Example 2, and the mass of Stephania tetrandra extract and citrus peel extract in the composition is replaced by an equal amount of patchouli leaf extract.

[0050] Comparative Example 4

[0051] A method for preparing a repairing and soothing composition differs from Example 2 in that it contains only Stephania tetrandra extract and Citrus peel extract. The preparation methods of Stephania tetrandra extract and Citrus peel extract are the same as in Example 2. The mass of patchouli leaf extract in the composition is replaced by an equal amount of Stephania tetrandra extract and Citrus peel extract, so that the mass ratio of Stephania tetrandra extract and Citrus peel extract in the composition is 1:1.

[0052] Comparative Example 5

[0053] A method for preparing a repairing and soothing composition differs from Example 2 in that it contains only Stephania tetrandra extract and Patchouli leaf extract. The preparation methods for Stephania tetrandra extract and Patchouli leaf extract are the same as in Example 2. The mass of citrus peel extract in the composition is replaced by an equal amount of Stephania tetrandra extract and Patchouli leaf extract, so that the mass ratio of Stephania tetrandra extract and Patchouli leaf extract in the composition is 1:1.

[0054] Comparative Example 6

[0055] The preparation method of a repairing and soothing composition differs from that of Example 2 in that it contains only citrus peel extract and patchouli leaf extract. The preparation methods of citrus peel extract and patchouli leaf extract are the same as in Example 2. The mass of Stephania tetrandra extract in the composition is replaced by an equal amount of citrus peel extract and patchouli leaf extract, so that the mass ratio of citrus peel extract and patchouli leaf extract in the composition is 1:1.

[0056] Comparative Example 7

[0057] A method for preparing a repairing and soothing composition, wherein the extract of Stephania tetrandra is extracted by ethanol reflux extraction (Stephania tetrandra powder and 70% ethanol aqueous solution are taken at a mass ratio of 1:10, refluxed at 80℃ for 2 hours, filtered, concentrated, and dried); the extract of citrus peel is extracted by water extraction (citrus peel powder and deionized water are taken at a mass ratio of 1:8, extracted at 90℃ for 1.5 hours, filtered, concentrated, and dried); and the extract of patchouli leaf is extracted by ethanol extraction (patchouli leaf raw material is taken, pulverized, and extracted by reflux at 65℃ for 1.5 hours with 60% ethanol aqueous solution, filtered, concentrated, and dried). The three extracts are mixed evenly at a mass ratio of 1:1:1 to obtain the composition.

[0058] Test Example 1: Skin Irritation Test

[0059] Human patch tests were conducted in accordance with the "Cosmetic Safety Technical Specifications" (2015 edition). The compositions prepared in Examples 1-3 were selected as experimental products. Thirty healthy subjects, half male and half female, were selected. Closed patch tests were performed on the inner forearm and behind the ear of the subjects. The test patch was removed after 24 hours. The skin was observed for irritation reactions such as erythema, edema, papules, and vesicles at 30 minutes, 24 hours, and 48 hours after removal.

[0060] The experimental results showed that the patch test results of all 30 subjects were negative, and no irritant reactions such as erythema, edema, papules, or vesicles occurred. This indicates that the compositions prepared in Examples 1-3 of this invention have no obvious irritant or sensitizing effects on the human body and have good safety performance.

[0061] Test Example 2: TEWL Test

[0062] Thirty subjects were randomly divided into 10 groups of 3 using a transdermal water loss analyzer. Under the same conditions, the compositions prepared in Examples 1-3 and Comparative Examples 1-7 (each prepared as a 5wt% solution with deionized water) were applied to the skin surface twice daily, morning and evening. TEWL values ​​were measured at the same skin site before use, and after 14 and 28 days of use. Each subject was tested three times, and the average value was calculated. The average TEWL reduction rate after 14 and 28 days of use for each group was calculated as follows: TEWL reduction rate (%) = (TEWL value before use - TEWL value after n days of use) / TEWL value before use × 100%. The average TEWL reduction rate (%) = the sum of TEWL reduction rates for each group / the number of subjects in that group. The results are shown in Table 1.

[0063] Table 1 TEWL Test Results

[0064]

[0065] TEWL value reflects skin barrier integrity; the higher the TEWL reduction rate, the better the skin barrier repair effect. When subjects continuously used the compositions of Examples 1-3 above, the skin TEWL value gradually decreased, the average TEWL reduction rate increased, and the effects of Examples 1-3 were significantly better than those of Comparative Examples 1-7, indicating that the compositions prepared in this invention have a good water-retention effect. This is closely related to the synergistic skin barrier repair effects of the three extracts: Stephania tetrandra extract, Citrus peel extract, and Patchouli leaf extract. The tetrandrine in Stephania tetrandra extract can inhibit the release of inflammatory factors, reduce the damage to the skin barrier caused by skin inflammation, and improve skin microcirculation, promoting water transport. Hesperidin in citrus peel extract can promote keratinocyte proliferation and enhance the structural stability of the stratum corneum. The polysaccharides in citrus peel extract have strong water-absorbing properties, locking in moisture. Patchouli ketone in patchouli leaf extract can regulate the skin microecology, reducing the invasion of harmful bacteria on the barrier. Patchouli leaf extract can also enhance the synthesis of ceramides in the stratum corneum. Ceramides are important lipids for maintaining the hydration state of the stratum corneum. These three components synergistically repair the skin barrier, thereby effectively reducing the TEWL value and improving skin water retention. However, comparative examples 1-6, lacking one or two of these extracts, showed a significantly reduced skin barrier repair effect. Comparative example 7, using the traditional solvent extraction process, had a smaller reduction in the TEWL value due to the low yield of the active ingredients.

[0066] Test Example 3: Stratum Corneum Moisture Content Test

[0067] Thirty subjects were randomly divided into 10 groups of 3 using a skin moisture meter. Under the same conditions, the compositions prepared in Examples 1-3 and Comparative Examples 1-7 (each prepared as a 5wt% solution with deionized water) were applied to the skin surface twice daily, morning and evening. The stratum corneum moisture content of the same skin area was measured before use, and after 14 and 28 days of use. Each subject was tested three times, and the average value was calculated. The average increase rate of stratum corneum moisture content after 14 and 28 days of use was calculated as follows: Moisture content increase rate (%) = (Moisture content after n days of use - Moisture content before use) / Moisture content before use × 100%. The average moisture content increase rate (%) = Sum of moisture content increase rates for each group / Number of subjects in that group. The results are shown in Table 2.

[0068] Table 2 Results of stratum corneum moisture content test

[0069]

[0070]

[0071] The stratum corneum moisture content reflects the skin's moisturizing ability; the higher the moisture content increase rate, the better the moisturizing effect. When subjects continuously used the compositions of Examples 1-3, the skin moisture content increase rate gradually increased, and the effects of Examples 1-3 were significantly better than those of Comparative Examples 1-7, indicating that the compositions prepared in this invention have excellent moisturizing and hydrating effects. This is attributed to the synergistic moisturizing and skin barrier repair mechanisms of the three extracts. Stephania tetrandra extract can improve skin microcirculation and promote water transport; the polysaccharide components in citrus peel extract have strong water absorption properties and can lock in moisture; patchouli leaf extract can enhance the synthesis of ceramides in the stratum corneum. Ceramides are important lipids for maintaining the hydration state of the stratum corneum; the three synergistically increase the stratum corneum moisture content. Comparative Example 7, using a traditional solvent extraction process, had a lower increase rate than the examples due to the low yield of effective components and insufficient water-retaining components.

[0072] Test Example 4: Skin Redness Improvement Test

[0073] Thirty subjects were randomly divided into 10 groups of 3 using a skin colorimeter. Under the same conditions, the compositions prepared in Examples 1-3 and Comparative Examples 1-7 (each prepared as a 5wt% solution with deionized water) were applied to the skin surface twice daily, morning and evening. The a-values ​​of the reddened areas on the subjects' faces were measured before use, and after 14 and 28 days of use. Each subject was tested three times, and the average value was calculated. The average improvement rate of skin redness after 14 and 28 days of use was calculated as follows: Redness improvement rate (%) = (a-value before use - a-value after n days of use) / a-value before use × 100%. The average redness improvement rate (%) = the sum of redness improvement rates in each group / the number of subjects in that group. The results are shown in Table 3.

[0074] Table 3 Results of the Skin Redness Improvement Test

[0075]

[0076] A decrease in the a-value of skin redness and an increase in the redness improvement rate indicate an improvement in skin inflammation and sensitivity. When subjects continued to use the compositions of Examples 1-3, the a-value improvement rate significantly increased, and skin redness was significantly improved, demonstrating a significant anti-inflammatory and soothing effect. This may be because the tetrandrine in the Stephania tetrandra extract can inhibit the release of inflammatory factors, reducing skin redness at its source; the hesperidin in the citrus peel extract has a strong antioxidant effect, which can scavenge free radicals and reduce inflammatory responses caused by oxidative stress; and the volatile oil components in the patchouli leaf extract can soothe nerve endings in the skin and reduce skin sensitivity. The three extracts work synergistically to reduce skin redness through multiple pathways, including inhibiting the release of inflammatory mediators, anti-oxidation, and soothing nerves. In contrast, Comparative Examples 1-6, which lack one or two of the extracts, can only exert anti-inflammatory and soothing effects at some stages, and their effects are worse than those of Examples 1-3. Comparative Example 7, due to the use of traditional extraction processes, has a low yield of effective ingredients and poor skin redness improvement.

[0077] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A method for preparing a repairing and soothing composition, characterized in that, Includes the following steps: (1) Preparation of Stephania tetrandra extract: Take Stephania tetrandra powder and deionized water at a mass ratio of 1:(8-12), mix and stir evenly, add compound enzyme solution, and enzymatically hydrolyze for 1-2 hours at 35-40℃ and pH 4.5-5.

0. After enzymatic hydrolysis, microwave-assisted extraction is performed with a microwave power of 240-360W and an extraction time of 20-25 minutes. After extraction, filter, concentrate and dry to obtain Stephania tetrandra extract. (2) Preparation of citrus peel extract: Citrus peel powder and deionized water were mixed and stirred evenly at a mass ratio of 1:(6-10). Lactobacillus plantarum was inoculated and fermented at 30-35℃ for 8-12 hours. After fermentation, the fermentation mixture was transferred to an extraction vessel. Deionized water was added at a mass ratio of citrus peel powder to deionized water of 1:(10-20). Subcritical water extraction was then carried out at an extraction temperature of 120-130℃ and a pressure of 3-4 MPa. After extraction, the mixture was filtered, concentrated and dried to obtain citrus peel extract. (3) Preparation of patchouli leaf extract: Take patchouli leaf raw material, crush it and add it into the extraction vessel. Add 65-75% ethanol aqueous solution at a rate of 0.05-0.15L / h as an entrainer and carry out supercritical CO2 extraction to obtain volatile oil. The residue after extraction was extracted by reflux with a 50%-70% ethanol aqueous solution at 60-70℃, filtered, concentrated and dried to obtain the non-volatile component extract; the volatile oil and the non-volatile component extract were mixed to obtain the patchouli leaf extract. (4) Mix the Stephania tetrandra extract obtained in step (1), the citrus peel extract obtained in step (2), and the patchouli leaf extract obtained in step (3) at a mass ratio of 1:(0.5-1.5):(0.5-1.5) to obtain a repair and soothing composition.

2. The preparation method according to claim 1, characterized in that, In step (1), the complex enzyme is xylanase and acidic protease, the mass ratio of xylanase to acidic protease is 1:(0.5-1), and the amount of the complex enzyme solution is 0.5-1 wt% of the mass of Stephania tetrandra powder.

3. The preparation method according to claim 1, characterized in that, In step (2), the inoculation amount of *Lactobacillus plantarum* is 0.4-0.8 wt% of the citrus peel powder.

4. The preparation method according to claim 1, characterized in that, In step (3), the supercritical CO2 extraction pressure is 25-30 MPa, the extraction temperature is 40-50℃, and the extraction time is 1-1.5 h.

5. The preparation method according to claim 1, characterized in that, In step (4), the mass ratio of the Stephania tetrandra extract, citrus peel extract, and patchouli leaf extract is 1:1:

1.

6. A repairing and soothing composition prepared by the method according to any one of claims 1-5.

7. The use of the repairing and soothing composition as described in claim 6 in the preparation of skin barrier repairing skin care products, characterized in that, The repairing and soothing composition is used in skincare products at a dosage of 0.5-5 wt%.

8. The application as described in claim 7, characterized in that, The skincare products include any one of creams, lotions, and serums.

Images