Cosmetic oil with whitening, soothing and anti-aging effects and preparation method thereof

Abstract

The invention discloses cosmetic oil with whitening, soothing and anti-aging effects and a preparation method of the cosmetic oil. The plant essential oil is prepared from the following raw materials in parts by weight: 0.5 to 1.5 parts of sunflower seed oil, 0.01 to 0.05 part of lupinus albus seed extract, 0.0001 to 0.001 part of tocopherol, 0.01 to 0.05 part of rosemary leaf extract, 0.001 to 0.1 part of dimethyl methoxy benzodihydropyranol, 50 to 90 parts of caprylic / capric triglyceride, 0.01 to 0.2 part of ascorbyl tetraisopalmitate, 0.01 to 0.2 part of bisabolol, 5 to 20 parts of squalane, 2 to 8 parts of simmondsia chinensis seed oil, 5 to 15 parts of meadowfoam seed oil and 0.1 to 1 part of lavender oil. And 0.3-1 part of a centella asiatica extract. According to the cosmetic oil product provided by the invention, by optimizing the use of substances, the composition has triple effects of whitening, relieving and resisting aging, can meet multiple skin care requirements of consumers at the same time, and is wide in market application range.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a cosmetic oil with whitening, soothing and anti-aging effects and a preparation method thereof. BACKGROUND

[0002] With the popularity of consumption upgrading and healthy lifestyle, consumers have higher requirements for the efficacy, safety and experience of skin care products. Cosmetic oils are favored due to their natural ingredients, high efficiency of nourishment and multi-functionality (such as moisturizing, anti-aging and barrier repair), and show an increasing market demand and market size. According to forecasts of some organizations, the global facial skin care oil market size will exceed 4 billion US dollars in 2030. Therefore, the research and development and production of different types of cosmetic oil products are valued by various companies.

[0003] Generally, the soothing, anti-aging and whitening effects of cosmetics are concerned by consumers. In the prior art, patent document CN119950366A provides an anti-aging cosmetic, grape pomace oil and a preparation method thereof, which comprises: grape pomace oil; the characteristic components of the grape pomace oil include: oleanolic acid, alpha-humulene, beta-tocotrienol, beta-sitosterol, campesterol, soybean saponin E, ganoderic acid F, ganoderol I and D-erythro-dihydrosphingosine; the total phenol content of the grape pomace oil is ≥1 mg / g, the total sterol content of the grape pomace oil is ≥26 mg / g, and the total flavonoid content of the grape pomace oil is ≥1 mg / g; the grape pomace oil is extracted from grape pomace; the grape pomace is composed of grape skin, grape stems and grape seeds.

[0004] For another example, patent document CN120189362A provides a cosmetic base oil rich in whitening active ingredients and a preparation method thereof, which comprises plant effective components and surfactants, and the amount of the surfactants is 1% to 5% of the mass of the plant effective components; the plant effective components include a plant raw material and a plant oil liquid in a weight ratio of 1:4 to 1:10. The plant oil refers to virgin oil or refined oil extracted from the fruits, seeds or germ of plants. The cosmetic base oil provided by the patent not only contains more whitening active ingredients, but also performs well in maintaining product stability.

[0005] For example, patent document CN116196246A provides a cosmetic composition with oil control, soothing and free radical scavenging effects and a preparation method thereof. The composition is obtained by co-fermenting gentian, capillaris, licorice, dried tangerine or orange peel and water using yeast and lactic acid bacteria, and then adding a stabilizing agent. The patent discloses a cosmetic composition obtained by co-fermenting gentian, capillaris, licorice, dried tangerine or orange peel and water using yeast and lactic acid bacteria, and then adding a stabilizing agent. At a lower concentration of 0.5% by mass, the composition shows a significant inhibitory effect on the secretion of oil by SZ95 human sebaceous gland cells, and has a significant oil control effect.

[0006] It can be seen that the cosmetic products provided in the above patent documents have relatively single functions, and it is necessary to provide a cosmetic oil with whitening, soothing and anti-aging triple effects, which has a good market sales prospect. Compared with traditional water-based whitening essence, oil-soluble whitening ingredients can more effectively penetrate the stratum corneum, target inhibit melanin production, reduce skin irritation, and are suitable for dry, sensitive and barrier-damaged skin.

[0007] Therefore, in view of the above problems, the present application is proposed. SUMMARY

[0008] In view of the above technical problems, the present application aims to provide a cosmetic oil with whitening, soothing and anti-aging effects and a preparation method thereof. The prepared cosmetic oil has multiple effects and has a good market application prospect.

[0009] To achieve the above-mentioned purpose, the present application adopts the following technical solutions: In a first aspect, the present application provides a cosmetic oil with whitening, soothing and anti-aging effects, which is prepared from the following raw materials in parts by weight: .

[0010] Ascorbic acid tetraisopalmitate is an oil-soluble VcOS that can be converted to Vc in cells. It is bioavailable and has better transdermal absorption effect than water-soluble Vc. It effectively inhibits melanin production in human melanoma cells and has inhibitory effect on intracellular tyrosinase activity.

[0011] Dimethylmethoxychromanol is a highly effective antioxidant that has dual scavenging effect on RNS and ROS. In addition, it has the effects of whitening and fading spots, protecting cells, resisting ultraviolet rays, inhibiting protein carbonylation and improving skin cell vitality.

[0012] Bisabolol has a very high skin soothing function. In vitro tests show that it has the same inhibitory effect on inflammatory factors as plant / chemical bisabolol, greatly reduces the three major pro-inflammatory cytokines, and has a high efficiency of more than 49%. It has an inhibition rate of up to 61% for IL-1 alpha.

[0013] Mei-er-yan (LIMNANTHES ALBA) seed oil contains 98% long-chain fatty acids (20+ carbon chains), most of which have an unsaturated double bond at the C-5 position, which is different from the more common C-9 and C-12 unsaturation of fatty acids such as oleic acid and linoleic acid, and has stronger stability; and contains natural antioxidants (natural tocopherols) Rosemary (ROSMARINUS OFFICINALIS) leaf extract can effectively reduce the secretion of inflammatory factors such as IL-6 and IL-8, effectively anti-wrinkle by promoting the content of elastin and hyaluronic acid, effectively reducing the production of AGEs, and achieving anti-glycation.

[0014] Sunflower (HELIANTHUS ANNUUS) seed oil effectively softens the skin, forming a protective film on the skin surface to prevent water loss, making the skin soft and smooth. The linoleic acid it contains is an important component of intercellular lipids, which helps to repair and maintain a healthy skin barrier, especially beneficial for dry, damaged or sensitive skin.

[0015] Jojoba (SIMMONDSIA CHINENSIS) seed oil is rich in vitamin E (tocopherol), a natural antioxidant that helps protect the skin from free radical damage; it contains rich long-chain fatty acids (such as erucic acid, eicosanoic acid) and fatty alcohols, which give it excellent lubricity and penetration.

[0016] Lavender (LAVANDULA ANGUSTIFOLIA) oil is rich in antioxidants that can fight free radicals and help reduce fine lines and keep skin elasticity.

[0017] White lupin (LUPINUS ALBUS) seed extract can stimulate the production of high-quality type I collagen and strengthen the extracellular matrix of the dermis that has lost type I collagen due to skin aging or damage (inflammation), helping to improve the mechanical properties of the skin: strong, tight, and elastic.

[0018] Tocopherol (vitamin E) is an important antioxidant that can scavenge free radicals (such as reactive oxygen species ROS) in the body, protecting cell membranes (especially polyunsaturated fatty acids) from oxidative damage. By binding to lipids in the cell membrane, it prevents lipid peroxidation and maintains cell membrane integrity. It works synergistically with vitamin C, glutathione, and other antioxidants to strengthen the antioxidant network.

[0019] The active ingredients in Centella asiatica extract can promote collagen synthesis and enhance skin barrier function; at the same time, asiatic acid and polyphenolic components can scavenge free radicals, activate fibroblasts, and improve skin elasticity; in addition, active substances such as asiatic acid can interfere with the melanin production pathway and reduce pigment deposition.

[0020] The above components realize the comprehensive effects of whitening, soothing and anti-aging by inhibiting the activity of tyrosinase, antioxidation, soothing anti-inflammation, promoting collagen synthesis and other pathways.

[0021] As a further preferred embodiment of the above technical solution, the cosmetic oil is prepared from the following raw materials by weight: .

[0022] As a further preferred embodiment of the above technical solution, the cosmetic oil is prepared from the following raw materials by weight: .

[0023] As a preferred embodiment of the above technical solution, the cosmetic oil is prepared from the following raw materials by weight: .

[0024] As a preferred embodiment of the above technical solution, the cosmetic oil is prepared from the following raw materials by weight: .

[0025] As a preferred embodiment of the above technical solution, the cosmetic oil is prepared from the following raw materials by weight: .

[0026] It should be particularly emphasized that the various substances provided in the above technical solution can be purchased through market channels or self-made. For example, as a preferred embodiment of the above technical solution, the preparation of the Centella asiatica extract includes the following steps: S1, the Centella asiatica is cleaned, dried and crushed, and then added to an aqueous malic acid solution for soaking treatment; S2, the soaked Centella asiatica is subjected to steam explosion treatment, and after the treatment is completed, the pressure is quickly released, and after filtration, a filtrate and a residue are obtained; S3, the residue obtained in step S2 is added to anhydrous ethanol containing Tween 80, and ultrasonic treatment is performed to obtain a secondary filtrate; S4, the secondary filtrate obtained in step S3 is separated by macroporous resin using anhydrous ethanol, and the separated solution is mixed with the filtrate obtained in step S2 to obtain the Centella asiatica extract.

[0027] In the technical solution, first, the Yunnan Rheum officinale is treated with malic acid, a weak organic acid, to preliminarily soften the cell wall and promote the dissolution of active ingredients (such as swertiamarin) and the stability of some triterpenoid components in an acidic environment; then, the Yunnan Rheum officinale is treated by steam explosion, which, in combination with the promotion of malic acid in the previous step, efficiently destroys the plant cell wall and fully releases intracellular substances; of course, for the treatment of the residue, Tween 80 is used to enhance the penetration of ethanol into liposoluble components (such as rheum acid) to fully improve the extraction efficiency. In the above extraction method, each substance is connected to ensure the extraction efficiency of Yunnan Rheum officinale.

[0028] As a preferred preparation method of the Yunnan Rheum officinale extract, in step S1, the Yunnan Rheum officinale is crushed to 80-150 mesh; the concentration of the malic acid aqueous solution is 6-8.5 g / L; the solid-liquid ratio of the Yunnan Rheum officinale and the malic acid aqueous solution is 1:8-15; the soaking temperature is room temperature, and the time is 2-6 h; In step S2, saturated water vapor is used for steam explosion treatment, the pressure of the steam explosion treatment is 1.05-1.15 MPa, and the treatment time is 60-90 s; In step S3, in anhydrous ethanol, the concentration of Tween 80 is 0.06-0.15 wt%; the dosage ratio of the residue and anhydrous ethanol is 1 g:5-10 mL; the ultrasonic treatment power is 100-150 W, and the treatment time is 15-30 min.

[0029] In the second aspect, the present application also provides a preparation method of the above cosmetic oil, which comprises the following steps: (1) dissolving the Lupinus albus seed extract in a first part of sunflower seed oil, adding tocopherol, and stirring uniformly to obtain phase A; (2) dissolving the Rosmarinus officinalis leaf extract in a second part of sunflower seed oil, adding dimethylmethoxybenzodihydropyranol, and stirring uniformly to obtain phase B; (3) uniformly mixing caprylic acid / capric acid triglyceride, ascorbic acid tetraisopalmitate, bisabalol, squalane, Simmondsia chinensis seed oil, Linum usitatissimum seed oil, Lavandula angustifolia oil, and Yunnan Rheum officinale extract to obtain phase C; (4) adding the obtained phase A, phase B, and phase C into an emulsifying tank, stirring uniformly, filtering, sealing, and aging to obtain the cosmetic oil.

[0030] As a preferred preparation method, in steps (1), (2), and (4), the stirring is performed at room temperature; In step (4), the filtering is performed using a 200-350 mesh filter.

[0031] Compared with the prior art, the present application has the following beneficial effects: (1) The cosmetic oil product provided by the present invention optimizes the use of substances so that the composition of the present invention has three effects of whitening, soothing and anti-aging, which can meet the multiple skin care needs of consumers at the same time and has a wide range of market applications.

[0032] (2) In the cosmetic oil product provided by the present invention, lupinol in the white lupin (LUPINUS ALBUS) seed extract can promote the production of type I collagen and has a good anti-aging effect. Sunflower seed oil, as a solvent carrier and bioactive enhancer of lupinol, can fully dissolve lupinol and enhance the efficiency of lupinol in penetrating the dermis. Tocopherol, as a stabilizer of lupinol, can effectively protect the chemical integrity of lupinol and significantly prolong the duration of action of lupinol.

[0033] (3) In the cosmetic oil product provided by the present invention, sunflower seed oil is used as a solvent for extracting rosemary leaf extract. Its high content of linoleic acid dissolves the fat-soluble active ingredients in rosemary leaf extract and improves the transdermal rate by softening the stratum corneum. At the same time, the present invention uses dimethylmethoxybenzodihydropyranol as a stabilizer to block photodegradation caused by ultraviolet rays and protect the ortho-dihydroxyl structure of rosemary polyphenols from oxidative inactivation. The three components work together to inhibit the inflammatory pathway, reduce the feedback generation of free radicals, and prolong the anti-oxidation and anti-aging effect of the system.

[0034] (4) The Centella asiatica extract used in the cosmetic oil product provided by the present invention is prepared by a specific method, with high extraction efficiency (high yield of flavonoids in the extracted extract), which ensures its efficacy in the cosmetic oil product. Detailed Implementation

[0035] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0036] Unless otherwise specified, all raw materials used in this invention can be purchased from the market and their specifications meet national pharmaceutical standards.

[0037] As partly exemplary, information on some of the substances used in the embodiments of the present invention is as follows: White lupinus (LUPINUS ALBUS) seed extract was purchased from Laboratoires Expanscience; Rosemary (ROSMARINUS OFFICINALIS) leaf extract was purchased from Shanghai Boshuo Industrial Co., Ltd. Jojoba (Simondisia Chinese) seed oil was purchased from Vantage Specialty Chemicals, Inc. The meadowfoam (Limenthanhes alba) seed oil was purchased from Xianting (Guangzhou) Trading Co., Ltd. The lavender (LAVANDULA ANGUSTIFOLIA) oil was purchased from Mutelli Immortelle (Albania) Ltd.

[0038] Example 1 A method for preparing Centella asiatica extract includes the following steps: S1. After washing and drying Centella asiatica, pulverize it to 100 mesh, then add it to a malic acid aqueous solution (7g / L) and soak it at room temperature for 2.5 days; the material-to-liquid ratio is 1:10. S2. The soaked Centella asiatica is subjected to steam explosion treatment (saturated steam, 1.1 MPa, 80s). After the treatment is completed, the pressure is quickly released, and the mixture is filtered to obtain filtrate and filter residue. S3. Add the filter residue obtained in step S2 to anhydrous ethanol containing Tween 80 (the concentration of Tween 80 is 0.1wt%), and sonicate (120W, 20min) to obtain a secondary filtrate; the ratio of filter residue to anhydrous ethanol is 1g:8mL. S4. Separate the secondary filtrate obtained in step S3 with anhydrous ethanol using macroporous resin. Mix the separated solution with the filtrate obtained in step S2 to obtain Centella asiatica extract.

[0039] Example 2 A cosmetic oil with whitening, soothing, and anti-aging effects is prepared by weight from the following raw materials: .

[0040] In this embodiment, a method for preparing the above-mentioned cosmetic oil is also provided, comprising the following steps: (1) Dissolve the white lupin seed extract in the first part of sunflower seed oil (accounting for 50% of the total mass), add tocopherol, and stir evenly at room temperature (30r / m) to obtain phase A; (2) Dissolve the rosemary leaf extract in the second part of sunflower seed oil (50% remaining), add dimethylmethoxybenzodihydropyranol, and stir evenly at room temperature (40 r / m) to obtain phase B; (3) After mixing caprylic / capric triglyceride, ascorbic acid tetraisopalmitate, bisabolol, squalane, jojoba seed oil, meadowfoam seed oil, lavender oil and centella asiatica extract evenly, phase C is obtained; (4) Add the obtained phase A, phase B and phase C to the emulsification tank, stir evenly at room temperature (35r / m), filter through a 300-mesh filter and seal, and age for 24 hours.

[0041] Example 3 A cosmetic oil with whitening, soothing, and anti-aging effects is prepared by weight from the following raw materials: .

[0042] In this embodiment, a method for preparing the above-mentioned cosmetic oil is also provided, comprising the following steps: (1) Dissolve the white lupin seed extract in the first part of sunflower seed oil (accounting for 50% of the total mass), add tocopherol, and stir evenly at room temperature (30r / m) to obtain phase A; (2) Dissolve the rosemary leaf extract in the second part of sunflower seed oil (50% remaining), add dimethylmethoxybenzodihydropyranol, and stir evenly at room temperature (40 r / m) to obtain phase B; (3) After mixing caprylic / capric triglyceride, ascorbic acid tetraisopalmitate, bisabolol, squalane, jojoba seed oil, meadowfoam seed oil, lavender oil and centella asiatica extract evenly, phase C is obtained; (4) Add the obtained phase A, phase B and phase C to the emulsification tank, stir evenly at room temperature (35r / m), filter through a 300-mesh filter and seal, and age for 24 hours.

[0043] Example 4 A cosmetic oil with whitening, soothing, and anti-aging effects is prepared by weight from the following raw materials: .

[0044] In this embodiment, a method for preparing the above-mentioned cosmetic oil is also provided, comprising the following steps: (1) Dissolve the white lupin seed extract in the first part of sunflower seed oil (accounting for 50% of the total mass), add tocopherol, and stir evenly at room temperature (30r / m) to obtain phase A; (2) Dissolve the rosemary leaf extract in the second part of sunflower seed oil (50% remaining), add dimethylmethoxybenzodihydropyranol, and stir evenly at room temperature (40 r / m) to obtain phase B; (3) After mixing caprylic / capric triglyceride, ascorbic acid tetraisopalmitate, bisabolol, squalane, jojoba seed oil, meadowfoam seed oil, lavender oil and centella asiatica extract evenly, phase C is obtained; (4) Add the obtained phase A, phase B and phase C to the emulsification tank, stir evenly at room temperature (35r / m), filter through a 300-mesh filter and seal, and age for 24 hours.

[0045] Comparative Example 1 Compared with Example 1, step S1 is omitted in Comparative Example 1, the process is adjusted accordingly, and the rest are the same.

[0046] Comparative Example 2 Compared with Example 1, in Comparative Example 2, step S3 omits the use of Tween 80 and uses only anhydrous ethanol. The process is adjusted accordingly, but the rest is the same.

[0047] Comparative Example 3 Compared with Example 2, the use of white lupinus albus seed extract was omitted, the process was adjusted accordingly, and the other components and preparation methods remained the same.

[0048] Comparative Example 4 Compared with Example 2, the use of tocopherol was omitted, the process was adjusted accordingly, and the other components and preparation methods were the same.

[0049] Comparative Example 5 Compared with Example 2, the use of dimethylmethoxybenzodihydropyranol was omitted, the process was adjusted accordingly, and the other components and preparation methods were the same.

[0050] Comparative Example 6 Compared with Example 2, the use of Centella asiatica extract was omitted, the process was adjusted accordingly, and the other components and preparation methods remained the same.

[0051] Experimental Example 1 The total flavonoid yield of the extracts obtained in Example 1, Comparative Examples 1 and 2 was calculated. The content was determined by ultraviolet spectrophotometry. The total flavonoid yield (%) was calculated as: (mass of flavonoids in the extract / mass of Centella asiatica used) × 100. The extraction results are shown in Table 1.

[0052] Table 1. Calculation results of total flavonoid yield in Example 1 and Comparative Examples 1-2 As can be seen from Table 1, the extraction method provided by this invention has a high yield of total flavonoids from Centella asiatica and high extraction efficiency.

[0053] Experimental Example 2 This experimental example refers to the standard "TSHRH 027-2019 In Vitro Test of B16 Cell Melanin Synthesis Inhibition Experiment" to determine the whitening efficacy of the products prepared in the examples and comparative proportions. B16 mouse melanoma cells are a commonly used cell model for studying melanin production. By measuring the change in melanin synthesis after the whitening raw materials act on melanocytes, the whitening activity of the raw materials can be evaluated.

[0054] 2.1 Main Reagents and Instruments B16 mouse melanoma cells (Cyber-Tech), CCK-8 kit (Solarbio), CO2 incubator (Esco), clean bench (Sujing Antai), microplate reader (Tecan), inverted microscope (Nikon).

[0055] 2.2 Test Methods A certain number of B16 cells are seeded into 96-well plates, typically 2 × 10⁶ cells. 4 Cells were cultured per well for 24 hours, then the stock solution was discarded. Test samples at different dose gradients were added, 100 μL per well. A solvent control group was also included. After another 24 hours of culture, the culture medium was discarded, and cytotoxicity was assessed according to the CCK-8 kit instructions. Cell viability was calculated. Based on the dose characteristics, cell viability, and cell morphology observed in the preliminary experiments, the sample dose (0.1 mg / mL) with a cell viability ≥80% was selected as the safe dose.

[0056] Samples at safe dosages were used as test groups for efficacy testing (samples from Example 2 were used as test group a, samples from Comparative Example 3 as test group b, samples from Comparative Example 4 as test group c, samples from Comparative Example 5 as test group d, and samples from Comparative Example 6 as test group e). A negative control group (cell culture medium) and a positive control group (0.1 mg / mL kojic acid solution) were also set up. Each group had 3 replicates. The absorbance at 405 nm of each well was measured using an ELISA reader according to the test method of the reference standard, and the melanin synthesis inhibition rate was calculated.

[0057] 1.3 Test Results The experimental results are shown in Table 2.

[0058] Table 2. Results of melanin synthesis inhibition rate of products in comparative and example cases. (Compared with the positive control group, *P<0.05, **P<0.01) As shown in Table 2, based on the effectiveness verification of the experiment, the melanin synthesis inhibition rate of experimental group a was significantly different from that of the positive control group. This indicates that the tested sample has the ability to inhibit melanin synthesis at the corresponding concentration, which can serve as evidence to support the product's whitening efficacy claim. Furthermore, the melanin synthesis inhibition effect of experimental group a was significantly better than that of other experimental groups, suggesting that the product corresponding to experimental group a has better whitening efficacy.

[0059] Experimental Example 3 This experiment followed the standard "T / SHRH 034-2021 Cosmetic Soothing Efficacy Test - In Vitro TNF-α Inflammatory Factor Content Determination Method for Lipopolysaccharide-Induced Macrophage RAW264.7" to determine the soothing efficacy of the products prepared in the examples and comparative examples. Specifically, it utilized the principle of bacterial lipopolysaccharide (LPS) inducing the secretion of the inflammatory factor TNF-α in the RAW264.7 macrophage cell line. The effect of the test substances on inhibiting TNF-α secretion was evaluated by measuring the differences in TNF-α content after treatment with different test substances. The specific evaluation indicators were TNF-α content and TNF-α inhibition rate. TNF-α content was calculated using enzyme-linked immunosorbent assay (ELISA), and the final TNF-α content result was taken as the average of three replicates for each group. Based on the dosage characteristics, cell viability, and cell morphology in the preliminary experiment, the sample dose (250 μg / mL) with a cell viability ≥90% was selected as the safe dose.

[0060] The samples at this safe dosage were used as the test groups for efficacy testing (samples from Example 2 were used as test group a, samples from Comparative Example 3 as test group b, samples from Comparative Example 4 as test group c, samples from Comparative Example 5 as test group d, and samples from Comparative Example 6 as test group e). The negative control group was given an equal volume of cell culture medium containing LPS, and the positive control group was given an equal volume of cell culture medium containing 100 μg / mL dexamethasone and LPS. The TNF-α content and TNF-α inhibition rate obtained from the products of the comparative examples and examples are shown in Table 3.

[0061] Table 3 Results of the inhibitory effect of the comparative and example products on TNF-α secretion (Compared with the negative control group, *P<0.05, **P<0.01) As shown in Table 3, based on the validation of the experiment, the TNF-α content in experimental group a was significantly different from that in the negative control group, indicating that the test sample has an inhibitory effect on TNF-α secretion, which can serve as evidence to support the product's claim of soothing efficacy. Furthermore, the TNF-α inhibition rate of experimental group a was higher than that of other experimental groups, suggesting that the product corresponding to experimental group a has better soothing efficacy.

[0062] Test Example 4 This experimental example refers to the standard "T / SHRH 031-2020 Cosmetic Firming and Anti-wrinkle Efficacy Test - In Vitro Fibroblast Type I Collagen Content Determination" to determine the firming and anti-wrinkle efficacy of the products prepared in the examples and comparative examples. Specifically, human dermal fibroblasts were used as a cell model to study the increase of type I collagen content. By measuring the upregulation rate of type I collagen content after administration of the test substance, compared with that of the blank control and the test substance, the efficacy of the test substance in promoting collagen synthesis was evaluated.

[0063] The specific evaluation indicators were type I collagen content and upregulation rate. The content was calculated after being measured by enzyme-linked immunosorbent assay (ELISA), and the final result of type I collagen content was the average of three replicates for each group. Based on the characteristics of each dose, cell viability and cell morphology in the preliminary experiment, the sample dose (250 μg / mL) with a cell viability ≥80% was selected as the safe dose.

[0064] 4.1 Main Reagents and Instruments Human dermal fibroblasts (Cyber-Kang), human dermal fibroblast culture medium (Cyber-Kang), transforming growth factor TGF-β1 (Cyber-Kang), human type I collagen enzyme-linked immunosorbent assay kit (Jinma), CCK-8 kit (Solarbio), CO2 incubator (Esco), clean bench (Sujing Antai), microplate reader (Tecan), inverted microscope (Nikon).

[0065] 4.2 Test Methods A certain number of HSF cells are seeded in a 96-well plate, typically 2 × 10⁶ cells / well. 4 Cells were cultured per well for 24 hours, then the stock solution was discarded. Different doses of the test sample were added, 200 μL per well. A solvent control group was also included. After another 24 hours of culture, the culture medium was discarded, and cytotoxicity was assessed according to the CCK-8 kit instructions. Cell viability was calculated. Based on the characteristics, cell viability, and cell morphology of each dose in the preliminary experiment, the sample dose (1250 μg / mL) with a cell viability ≥80% was selected as the safe dose.

[0066] Samples at safe dosages were used as the experimental groups for efficacy testing (samples from Example 2 were used as experimental group a, samples from Comparative Example 3 as experimental group b, samples from Comparative Example 4 as experimental group c, samples from Comparative Example 5 as experimental group d, and samples from Comparative Example 6 as experimental group e). A negative control group (cell culture medium) and a positive control group (TGF-β1 solution) were also included. Each group had three replicates. The optical density (OD value) was measured at 450 nm using a microplate reader according to the reference standard test method, and the type I collagen content was calculated. The formula for calculating the upregulation rate of type I collagen content is: Upregulation rate (%) = ((T-SC) / SC) × 100%; where T is the average type I collagen content of the tested samples, and SC is the average type I collagen content of the negative control.

[0067] 4.3 Test Results The experimental results are shown in Table 4.

[0068] Table 4. Results of Type I Collagen Content Detection in Comparative and Example Products (Compared with the negative control group, *P<0.05, **P<0.01) As shown in Table 4, based on the validation of the experiment, compared with the positive control, the content of type I collagen in experimental group a was significantly increased (P < 0.05), indicating that the experimental group at a concentration of 1.25 mg / mL has the ability to promote the synthesis of type I collagen, which can serve as evidence to support the product's claims of firming and anti-wrinkle effects. Furthermore, the rate of increase in type I collagen content in experimental group a was higher than that in other experimental groups, suggesting that the product corresponding to experimental group a has better firming and anti-wrinkle effects.

[0069] Experimental Example 5 Next, we will conduct a human efficacy evaluation experiment. The specific experimental method is as follows.

[0070] 5.1 Evaluation Indicators 5.1.1 Skin elasticity R2 value Measuring instrument: Cutometer MPA580 (Courage & Khazaka, Germany). Measurement requirements: 3 tests were performed and the average value was taken.

[0071] Parameter explanation: The higher the R2 value, the better the skin elasticity. Unit: dimensionless.

[0072] 5.1.2 Skin firmness F4 value Measuring instrument: Cutometer MPA580 (Courage & Khazaka, Germany). Measurement requirements: 3 tests were performed and the average value was taken.

[0073] Parameter explanation: The lower the F4 value, the better the skin firmness. Unit: dimensionless.

[0074] 5.1.3 Skin luster Measuring instrument: Glossymeter GL200 skin glossiness probe (Courage & Khazaka, Germany). Measurement requirements: 3 tests were performed and the average value was taken.

[0075] Parameter explanation: The higher the value, the more radiant the skin. Unit: dimensionless.

[0076] 5.1.4 Erythema Index (EI) value Measuring instrument: Mexameter MX 18 (Courage & Khazaka, Germany) for measuring skin melanin and hemoglobin. Measurement requirements: Take the average of 3 tests.

[0077] Parameter Explanation: The EI value represents the relative content of hemoglobin in the skin. A higher value indicates a higher relative content of hemoglobin in the skin, and a lower value indicates a lower relative content of hemoglobin in the skin. Unit: dimensionless.

[0078] 5.2 Test Methods Inclusion criteria for participants: Sixty healthy Chinese participants aged 25-50 years with significant periorbital dark circles and an ITA value difference of ≥1° compared to adjacent facial areas were selected. They were divided into three groups of 20 participants each (corresponding to Example 2, Comparative Example 4, and Comparative Example 5), and used the test product continuously for 28 days. Testing was conducted on days 14 and 28 of product use, and the results were statistically analyzed using SPSS software.

[0079] Subjects should cleanse their face and the skin around their eyes every morning and evening. After the skin around the eyes has dried naturally, apply an appropriate amount of facial oil evenly to the skin around the eyes and gently massage until absorbed. During the entire testing period, subjects should avoid prolonged sun exposure, outdoor sports, travel, etc., and should not use cosmetics or medications with similar effects to the product. Subjects should also not change their daily skincare routine during the testing period.

[0080] The test environment was a temperature of 20.0℃~22.0℃ and a humidity of 40%~60%RH.

[0081] 5.3 Test Results 5.3.1 Results of skin elasticity R2 value test The results of the skin elasticity R2 value test are shown in Table 5 below. As can be seen from the table, compared with before using the product, after using the product in the example for 28 days, the skin elasticity R2 value at the corner of the eye improved significantly by 25.5%, indicating that the product can effectively enhance skin elasticity; in contrast, the improvement in skin elasticity R2 in the comparative example was relatively weak.

[0082] Table 5. Results of skin elasticity R2 values ​​of subjects before and after product use (X±S, n=20) (Compared with before product use, 0.01 ≤ *P < 0.05, **P < 0.01) 5.3.2 Skin Tightness F4 Value Test Results The results of the skin firmness F4 value test are shown in Table 6 below. As can be seen from the table, compared with before using the product, after using the product in the example for 28 days, the skin firmness F4 value at the corner of the eyes decreased significantly by 29.2%, indicating that the product can effectively enhance the firmness of the skin at the corner of the eyes; in contrast, the improvement in skin firmness F4 value of the comparative product was relatively weak.

[0083] Table 6. Results of skin firmness F4 value test before and after product use (X±S, n=20) (Compared with before product use, 0.01 ≤ *P < 0.05, **P < 0.01) 5.3.3 Skin Glossiness Test Results The results of the skin gloss test are shown in Table 7 below. As can be seen from the table, compared with before using the product, after using the product in the example for 28 days, the skin gloss improved significantly by 47.7%, indicating that the product can effectively enhance skin gloss. In contrast, the skin gloss improvement effect of the comparative product is relatively weak.

[0084] Table 7. Results of skin radiance test before and after product use (X±S, n=20) (Compared with before product use, 0.01 ≤ *P < 0.05, **P < 0.01) 5.3.4 Results of Erythema Index (EI) Test The results of the Erythema Index (EI) test are shown in Table 8 below. As can be seen from the table, compared to before product use, after 28 days of using the product in this embodiment, the EI value decreased significantly by 28.4%, indicating that this product has a good effect on repairing the skin around the eyes and improving skin erythema reactions. After 28 days of using the comparative product, the change rate of the EI value was not as high as that of the product in this embodiment. Therefore, it can be concluded that the product in this embodiment has a better soothing effect on the skin around the eyes than the comparative product.

[0085] Table 8. Results of Erythema Index (EI) values ​​of the subjects' skin before and after product use (X±S, n=10) (Compared with before product use, 0.01 ≤ *P < 0.05, **P < 0.01) 5.4 Experimental Conclusions Compared to before using the test product, after 2 and 4 weeks of use of the cosmetic oil prepared in the example, the subjects showed a significant increase in the R2 value of skin elasticity at the corners of their eyes and a significant improvement in the F4 value of skin firmness at the corners of their eyes, indicating that the product of this invention has good anti-aging effects. Compared to before using the test product, after 2 and 4 weeks of use of the cosmetic oil prepared in the example, the subjects showed a significant improvement in the luster of the skin at the corners of their eyes, indicating that the product of this invention has good whitening effects. Compared to before using the test product, after 2 and 4 weeks of use of the cosmetic oil prepared in the example, the subjects showed a significant improvement in the EI value of the erythema index of the skin under the eyes, indicating that the product of this invention has good anti-inflammatory and soothing effects. Furthermore, the adverse reaction evaluation results showed that no subjects experienced any skin adverse reactions related to the use of the product during the trial period.

[0086] In summary, the product of this invention has good whitening, anti-aging, and soothing effects, with significant overall benefits and high safety.

[0087] The applicant declares that the present invention is illustrated by the above embodiments, but the present invention is not limited to the above embodiments, that is, it does not mean that the present invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions of individual raw materials in the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

Claims

1. A cosmetic oil with whitening, soothing, and anti-aging effects, characterized in that, It is prepared from the following raw materials in parts by weight: 。 2. The cosmetic oil with whitening, soothing, and anti-aging effects according to claim 1, characterized in that, It is prepared from the following raw materials in parts by weight: 。 3. The cosmetic oil with whitening, soothing, and anti-aging effects according to claim 1, characterized in that, It is prepared from the following raw materials in parts by weight: 。 4. The cosmetic oil with whitening, soothing, and anti-aging effects according to claim 1, characterized in that, It is prepared from the following raw materials in parts by weight: 。 5. A cosmetic oil with whitening, soothing, and anti-aging effects according to claim 1, characterized in that, It is prepared from the following raw materials in parts by weight: 。 6. The cosmetic oil with whitening, soothing, and anti-aging effects according to claim 1, characterized in that, It is prepared from the following raw materials in parts by weight: 。 7. A cosmetic oil with whitening, soothing, and anti-aging effects according to claim 1, characterized in that, The preparation of the Centella asiatica extract includes the following steps: S1. After washing and drying the Centella asiatica, crush it and then add it to the malic acid solution for soaking treatment; S2. The soaked Centella asiatica is subjected to steam explosion treatment. After the treatment is completed, the pressure is quickly released, and after filtration, filtrate and filter residue are obtained. S3. Add the filter residue obtained in step S2 to anhydrous ethanol containing Tween 80, and sonicate to obtain a secondary filtrate. S4. Separate the secondary filtrate obtained in step S3 with anhydrous ethanol using macroporous resin. Mix the separated solution with the filtrate obtained in step S2 to obtain Centella asiatica extract.

8. A cosmetic oil with whitening, soothing, and anti-aging effects according to claim 5, characterized in that, In step S1, Centella asiatica is pulverized to 80-150 mesh; the concentration of malic acid aqueous solution is 6-8.5 g / L; the ratio of Centella asiatica to malic acid aqueous solution is 1:8-15; the soaking temperature is room temperature and the soaking time is 2-6 hours. In step S2, saturated steam is used for the steam explosion treatment. The pressure of the steam explosion treatment is 1.05~1.15MPa, and the treatment time is 60~90s. In step S3, the concentration of Tween 80 in anhydrous ethanol is 0.06~0.15wt%; the ratio of filter residue to anhydrous ethanol is 1g:5~10mL; the ultrasonic treatment power is 100~150W, and the treatment time is 15~30min.

9. A method for preparing the cosmetic oil according to any one of claims 1 to 8, characterized in that, Includes the following steps: (1) Dissolve white lupin seed extract in the first part of sunflower seed oil, add tocopherol, stir well to obtain phase A; (2) Dissolve rosemary leaf extract in the second part of sunflower seed oil, add dimethylmethoxybenzodihydropyranol, stir well to obtain phase B; (3) After mixing caprylic / capric triglyceride, ascorbic acid tetraisopalmitate, bisabolol, squalane, jojoba seed oil, meadowfoam seed oil, lavender oil and centella asiatica extract evenly, phase C is obtained; (4) Add the obtained phase A, phase B and phase C to the emulsification tank, stir evenly, filter and seal, and then age.

10. The preparation method according to claim 9, characterized in that, In steps (1), (2), and (4), stirring is carried out at room temperature. In step (4), a 200-350 mesh filter is used for filtration.

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