Application of 3-acetylthiophene in prevention and treatment of aphids
By using 3-acetylthiophene hexane solution on cucumbers, the "3R" problem and the limitations of volatile organic compound application in existing aphid control technologies have been solved, achieving efficient aphid control and enhancing plant insect resistance.
Patent Information
- Application Number
- CN202511184443.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-22
- Publication Date
- 2025-12-05
AI Technical Summary
Existing technologies for controlling aphids suffer from the "3R" problem, and the application of volatile organic compounds is limited, making it difficult to effectively guide plant defense responses to improve insect resistance.
3-Acetylthiophene was used as a repellent. It was dissolved in hexane to form a solution and applied to cucumber leaves, especially during the nymphal stage of aphids. The repellent effect of its natural volatile substances reduced the attraction of aphids.
It effectively reduces the attraction of aphids, enhances the plant's resistance to insects, reduces the use of chemical pesticides, and achieves efficient control of aphids.
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Figure CN121058664A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of agricultural pest control, and particularly relates to application of 3-acetyl thiophene in prevention and treatment of aphids. BACKGROUND
[0002] Cucumber (Cucumis sativus L.) is a kind of annual climbing herbaceous plant of Cucurbitaceae and is one of the important vegetable crops in China and also is an important economic vegetable crop in the world. The fruits of cucumber can be used to extract various free amino acids, volatile oil, cucurbitacin and cucumber enzyme, which are widely used in cosmetic, food and medicine industries and have high economic and health values.
[0003] Aphid is a general term for the superfamily of Aphidoidea in Hemiptera, and there are about 4400 species in total, most of which belong to the family Aphididae and are widely distributed in the world. Aphids cause 5%-20% reduction in annual grain crops and are one of the top ten economic pests in the world.
[0004] Studies have shown that aphids harm crops such as chrysanthemum, melon, eggplant and pepper, especially in the production period of cucumber, which produces a large amount of honeydew on the tender parts of cucumber, causing soot disease, affecting photosynthesis of plants, and further leading to nutrient deficiency of plants. In addition, aphids can also transmit viruses, causing plants to wilt or die, which is very harmful in production. The common method in production is to spray pesticides, which may cause the "3R" problem.
[0005] When plants are subjected to diseases, pests, mechanical damage or other environmental stresses, they release specific volatile organic compounds (VOCs). These compounds can spread in the air and be perceived by surrounding plants, triggering their defense mechanisms. For example, ethylene and jasmonic acid, the release of these volatile substances can be used to warn neighboring plants and induce a series of defense responses, such as increasing the synthesis of insect-resistant proteins and enhancing the strength of cell walls, so that they can prepare in advance and improve their resistance to pests. SUMMARY
[0006] In view of this, in order to solve the limitations of existing volatile organic compounds in the prevention and treatment of aphids and to explore novel aphid-resistant substances, the present application provides application of 3-acetyl thiophene in prevention and treatment of aphids.
[0007] The application of 3-acetyl thiophene in prevention and treatment of aphids, 3-acetyl thiophene is used as a repellent to prevent and treat aphids.
[0008] Further, the application of 3-acetyl thiophene in prevention and treatment of aphids includes: dissolving 3-acetyl thiophene in n-hexane to prepare a n-hexane solution of 3-acetyl thiophene, and using the n-hexane solution of 3-acetyl thiophene for prevention and treatment of aphids.
[0009] Further, the mass concentration of 3-acetyl thiophene in the n-hexane solution of 3-acetyl thiophene is 0.01%-1%.
[0010] Further, the mass concentration of 3-acetyl thiophene in the n-hexane solution of 3-acetyl thiophene is 0.09%-0.11%.
[0011] Further, the mass concentration of 3-acetyl thiophene in the n-hexane solution of 3-acetyl thiophene is 0.1%.
[0012] Further, the n-hexane solution of 3-acetyl thiophene is used for preventing and treating vegetable aphids.
[0013] Further, the vegetable is cucumber.
[0014] Further, the aphid is cotton aphid.
[0015] Further, the application method comprises: applying the n-hexane solution of 3-acetyl thiophene on the leaves of the vegetable, preferably applying the n-hexane solution of 3-acetyl thiophene in the nymph stage of the aphid, more preferably applying the n-hexane solution of 3-acetyl thiophene in the 2-3 instar apterous nymph stage.
[0016] Compared with the prior art, the present application at least has the following beneficial effects:
[0017] The application provides the application of 3-acetyl thiophene in preventing and treating aphids, wherein 3-acetyl thiophene is a natural volatile organic compound released by cucumber, and 3-acetyl thiophene plays a role of reducing the attraction effect on aphids and has a certain repellent effect on aphids. BRIEF DESCRIPTION OF DRAWINGS
[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.
[0019] Figure 1 The figure shows the attraction effect of different concentrations of 3-acetyl thiophene on aphids in the embodiment 1 of the present application. DETAILED DESCRIPTION
[0020] In order to better understand the above technical solutions, the technical solutions of the embodiments of the present application are described in detail below through the drawings and specific embodiments. It should be understood that the specific features in the embodiments of the present application and the specific features in the embodiments are detailed descriptions of the technical solutions of the embodiments of the present application, rather than limitations of the technical solutions of the present application. In the case of no conflict, the technical features in the embodiments of the present application and the embodiments can be combined with each other.
[0021] The application provides the application of 3-acetyl thiophene in the prevention and control of aphids, and 3-acetyl thiophene is used as a repellent to prevent and control aphids.
[0022] Research has found that 3-acetyl thiophene is a natural volatile organic compound released by stressed cucumbers, and 3-acetyl thiophene can play a role in reducing the attraction effect on aphids and has a certain repellent effect on aphids.
[0023] In some embodiments, 3-acetyl thiophene is dissolved in n-hexane to prepare a n-hexane solution of 3-acetyl thiophene, and the n-hexane solution of 3-acetyl thiophene is used to prevent and control aphids.
[0024] Specifically, n-hexane has no effect on aphids itself and is a good solvent for dissolving 3-acetyl thiophene.
[0025] In some embodiments, the mass concentration of 3-acetyl thiophene in the n-hexane solution of 3-acetyl thiophene is 0.01%-1%; preferably, the mass concentration of 3-acetyl thiophene in the n-hexane solution of 3-acetyl thiophene is 0.09%-0.11%; more preferably, the mass concentration of 3-acetyl thiophene in the n-hexane solution of 3-acetyl thiophene is 0.1%.
[0026] Specifically, the attraction effect of 3-acetyl thiophene with different concentrations on aphids is tested by a four-arm olfactometer, and the results show that the attraction effect of 3-acetyl thiophene on aphids is significantly reduced relative to the control when the mass concentration of 3-acetyl thiophene is 0.01%-1%, and the attraction effect of the n-hexane solution of 3-acetyl thiophene with a mass concentration of 0.1% is the lowest, and 3-acetyl thiophene has a certain repellent effect on aphids.
[0027] In some embodiments, the n-hexane solution of 3-acetyl thiophene is used to prevent and control vegetable aphids, preferably cucumbers, and preferably cotton aphids.
[0028] In some embodiments, the application method comprises: applying the n-hexane solution of 3-acetyl thiophene on the leaves of vegetables; preferably, the n-hexane solution of 3-acetyl thiophene is applied in the nymph stage of aphids; more preferably, the n-hexane solution of 3-acetyl thiophene is applied in the 2-3 instar apterous nymph stage.
[0029] Specifically, the prepared 3-acetyl thiophene n-hexane solution is directly sprayed or applied to the leaves of the vegetable crops in need of protection. By accurately applying the pesticide at the most sensitive and critical (pre-adult formation) development stage of the aphids (2-3 instar apterous nymphs), the repelling effect is maximized, thereby effectively inhibiting the establishment and growth of the aphid population and protecting the vegetable crops.
[0030] The application of 3-acetyl thiophene in inducing aphid resistance in vegetables will be further described below in combination with the accompanying drawings and Example 1.
[0031] Example 13-acetyl thiophene in the application of preventing and treating vegetable aphids
[0032] (I) The application of 3-acetyl thiophene in preventing and treating vegetable aphids, wherein the attracting experiment comprises the following steps:
[0033] (1) Select 2-3 instar apterous nymphs and starve them for two hours;
[0034] (2) Dissolve 3-acetyl thiophene in n-hexane to prepare 3-acetyl thiophene n-hexane solutions with mass concentrations of 1%, 0.1%, and 0.01%, respectively.
[0035] (3) Perform indoor test experiment. The indoor test uses a four-arm olfactometer to test the behavioral response of aphids to the volatile substance 3-acetyl thiophene after the cucumber is stressed by aphids. The four-arm olfactometer is used to test the attracting activity of 3-acetyl thiophene with three concentrations to aphids. The specific test method in the indoor experiment is as follows: five minutes at a time, five aphids, thirty times a group, and three groups in total. After the three groups, compare with the control n-hexane, and count the number of aphids crossing the line. In each experiment, three areas are used as treatment areas, 20 μl of 3-acetyl thiophene n-hexane solution with different mass concentrations is absorbed and dropped on sterile filter paper cut into the same size and placed in the collection bottle (the test material is replaced after each experiment during the test process), and the remaining one area is used as the control, 20 μl of n-hexane is absorbed and dropped on sterile filter paper cut into the same size and placed in the collection bottle, and the reaction of aphids is observed.
[0036] (II) Result discussion: During the test process, aphids staying in the base arm are considered not to have reacted, and aphids entering the collection bottle are considered to have selectively reacted. After each test, all pipelines of the olfactometer are washed with alcohol to avoid mutual influence between the two experiments. After each experiment, the number of aphids entering the collection bottle is counted.
[0037] The results are shown in Table 1. Figure 1 As shown in Table 1, the results show that, in the attracting test of aphids by the four-arm olfactometer, the overall attracting activity of 3-acetyl thiophene n-hexane solution with a mass concentration of 0.1% to aphids is significantly lower than that of other concentrations. Figure 1The middle P value represents the difference significance level, and P<0.05 represents significant difference.
[0038] In summary, the application provides the application of 3-acetyl thiophene in the prevention and treatment of aphids. The attraction effect of 3-acetyl thiophene at three concentrations on aphids is tested by four-arm olfactometer, wherein the 0.1% 3-acetyl thiophene solution in n-hexane has the lowest attraction effect and has a certain repelling effect on aphids.
[0039] The above is only a preferred embodiment of the present application, and is not used to limit the present application, any modification, equivalent replacement and improvement made within the spirit and principle of the present application should be included in the protection scope of the present application. The above is only a preferred embodiment of the present application, and it should be pointed out that, for ordinary skilled in the art, without departing from the technical principle of the present application, a number of improvements and variations can also be made, which should be regarded as the protection scope of the present application.
Claims
1. Use of 3-acetylthiophene for controlling aphids, characterized in that, 3-acetylthiophene is used as a repellent to control aphids.
2. Use of 3-acetylthiophene according to claim 1 for controlling aphids, characterized in that, 3-acetylthiophene is dissolved in n-hexane to form a 3-acetylthiophene n-hexane solution, and the 3-acetylthiophene n-hexane solution is used to control aphids.
3. Use of 3-acetylthiophene according to claim 2 for controlling aphids, characterized in that, The mass concentration of 3-acetylthiophene in the 3-acetylthiophene n-hexane solution is 0.01%-1%.
4. Use of 3-acetylthiophene according to claim 3 for controlling aphids, characterized in that, The mass concentration of 3-acetylthiophene in the 3-acetylthiophene n-hexane solution is 0.09%-0.11%.
5. Use of 3-acetylthiophene according to claim 3 for controlling aphids, characterized in that, The mass concentration of 3-acetylthiophene in the 3-acetylthiophene n-hexane solution is 0.1%.
6. Use of 3-acetylthiophene according to any one of claims 2 to 5 for controlling aphids, characterized in that, The 3-acetylthiophene n-hexane solution is used to control vegetable aphids.
7. Use of 3-acetylthiophene according to claim 6 for controlling aphids, characterized in that, The vegetable is cucumber.
8. Use of 3-acetylthiophene according to claim 6 for controlling aphids, characterized in that, The aphid is Aphis gossypii.
9. Use of 3-acetylthiophene according to claim 6 for controlling aphids, characterized in that, The application method comprises applying the 3-acetylthiophene n-hexane solution on the leaves of the vegetable.
10. Use of 3-acetylthiophene according to claim 9 for controlling aphids, characterized in that, The application period is the nymph stage of the aphid.