Nutrient composition for improving muscle function and application

Abstract

The invention relates to the technical field of nutritional foods, and particularly discloses a nutritional composition for improving muscle functions and application. The nutritional composition for improving the muscle function, provided by the invention, is prepared from the following components in parts by mass: 40 to 60 parts of 1, 3-dioleic acid-2-palmitic acid triglyceride and 20 to 70 parts of 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride. A specific amount of OPO and OPL are compounded, and the two components have a synergistic effect, so that the exercise amount and the ATP content of an organism are remarkably increased, sarcopenia is relieved by improving energy metabolism, the muscle function is improved, and the food has a wide application prospect in the field of food and is worthy of popularization and application. The food can be used in health-care food, formula food for special medical purposes, infant formula food or special diet food.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of nutritional food, in particular to a nutritional composition for improving muscle function and application. BACKGROUND

[0002] The early development of infants is very important, especially the development of large muscle movement. Large muscle movement refers to the movement of infants using large muscle groups of each part of the body. Through large muscle movement, infants can promote the coordinated development of each part of the body, lay the foundation for future walking and movement development. Large muscle movement can help babies strengthen muscle strength and stretching ability, promote bone development; large muscle movement can increase the balance and coordination of babies, improve body control ability, reduce the risk of falling and injury; in addition, through large muscle movement, babies can also improve heart and lung function, enhance resistance, and promote overall healthy development.

[0003] Immunoglobulin can improve the body's ability to resist viral and bacterial infections from the outside world, thereby increasing the body's own immunity. Among them, immunoglobulin G (IgG) is the only protein that can passively immunize the young through the mother, can promote the digestion and absorption of nutrients through the regulation of inflammation, metabolism and other pathways, and participate in skeletal muscle energy metabolism pathways.

[0004] Human milk contains rich nutrients, and fat provides about 50% of the dietary energy for the growth and development of infants, and participates in the rapid growth of multiple organs of infants. The efficient absorption and rapid energy supply characteristics of human milk fat are crucial for infants with fast metabolism and small stomach capacity, and can immediately meet the high energy demand of tissues including muscles. Human milk fat mainly exists in the form of triglycerides, more than 70% of palmitic acid is combined at the sn-2 position of the glycerol skeleton, and the main unsaturated fatty acids such as oleic acid and linoleic acid occupy the sn-1,3 position, forming a special sn-2 palmitate. 1,3-dioleic acid-2-palmitic acid triglyceride (OPO) and 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride (OPL) are the two most abundant sn-2 palmitates in human milk. Existing research shows that OPO and OPL can effectively improve calcium absorption, promote infant bone development, reduce soaps, and improve infant intestinal comfort. So far, there has been no research on the effect of IgG, OPO or OPL on increasing the ATP content of the body and improving muscle function, so it is of great significance to study and explore the specific relationship between them. SUMMARY

[0005] In view of the above problems, the present application provides a nutritional composition for improving muscle function and application. By compounding OPO and OPL in a specific ratio, the ATP content of the body can be increased, and the muscle function can be improved.

[0006] To solve the above technical problems, the technical scheme provided by the present application is: In a first aspect, the present application provides a nutritional composition for improving muscle function, comprising the following components in mass fraction: 1,3-dioleic acid-2-palmitic acid triglyceride 40-60 parts and 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride 20-70 parts.

[0007] Compared with the prior art, the nutritional composition for improving muscle function provided by the present application significantly increases the exercise amount and ATP content of the body by compounding specific amounts of OPO and OPL, and improves muscle function by improving energy metabolism.

[0008] Preferably, the mass ratio of the 1,3-dioleic acid-2-palmitic acid triglyceride and the 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride is 1:(0.4-1.8).

[0009] Further preferably, the mass ratio of the 1,3-dioleic acid-2-palmitic acid triglyceride and the 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride is 1:(0.5-1.5).

[0010] Preferably, the nutritional composition further comprises the following components in mass fraction: immunoglobulin G 5-30 parts.

[0011] Further preferably, the nutritional composition comprises the following components in mass fraction: immunoglobulin G 10-20 parts, 1,3-dioleic acid-2-palmitic acid triglyceride 40-50 parts, and 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride 30-50 parts.

[0012] The preparation method of the nutritional composition for improving muscle function of the present application is not particularly limited, and the components are simply mixed uniformly.

[0013] In a second aspect, the present application provides the use of the nutritional composition for improving muscle function in the preparation of food.

[0014] Preferably, the food comprises at least one of health food, special medical purpose formula food, infant formula food, or special dietary food.

[0015] Further preferably, the food comprises at least one of infant formula milk powder, infant complementary food, child formula milk powder, pregnant and lying-in woman milk powder, middle-aged and old person milk powder, liquid milk, nutritional supplement, or dietary supplement.

[0016] The present application has the following beneficial effects: The present application significantly increases the ATP content of the body and improves muscle function by compounding specific amounts of IgG, OPO and OPL. As can be seen from the test results of the examples, when IgG, OPO or OPL is used alone, it cannot improve the sarcopenia of zebrafish and cannot effectively increase the ATP content in the body of zebrafish; only using OPO and OPL compounding has an improvement effect on the moving distance of zebrafish, and can also significantly increase the ATP content in the body of zebrafish, and the improvement effect is different with the change of the dosage ratio of OPO and OPL; using IgG, OPO and OPL compounding, the moving distance of zebrafish is significantly increased, and the ATP content in the body of zebrafish is also significantly increased. This shows that the components can synergistically relieve sarcopenia, improve energy metabolism, and thus promote the muscle function of the body. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 Comparison chart of the moving distance of zebrafish in each group in the verification test of the present application; Figure 2 Comparison chart of the ATP content in the body of zebrafish in each group in the verification test of the present application; In the figure, * represents p<0.05 compared with the model control group, ** represents p<0.01 compared with the model control group, and *** represents p<0.001 compared with the model control group. DETAILED DESCRIPTION

[0018] In order to make the purpose, technical scheme and advantages of the present application more clear and explicit, the present application will be further described in detail below in combination with examples. It should be understood that the specific examples described here are only used to explain the present application and do not limit the present application.

[0019] In the examples and comparative examples of the present application, IgG is purchased from Shanghai Yuanye Biotechnology Co., Ltd., OPO is purchased from Sigma Company, and OPL is purchased from Sigma Company. Other manufacturers not specified are products that can be obtained by purchase in the market.

[0020] In order to better illustrate the present application, further example is made by example below.

[0021] Example 1 The present example provides a nutritional composition for improving muscle function, which is composed of OPO and OPL with a mass ratio of 60:24.

[0022] Dimethyl sulfoxide (DMSO) is used to prepare OPO and OPL into 10.0 mg / mL stock solution respectively, and after adjusting the mass ratio, standard dilution water is added to prepare a nutritional composition solution with a total concentration of 500 μg / mL, which is ready for use.

[0023] Example 2 The embodiment provides a nutritional composition for improving muscle function, which is composed of OPO and OPL with a mass ratio of 45:36.

[0024] The OPO and the OPL are respectively prepared into 10.0 mg / mL mother liquor by using DMSO, and then are mixed according to the mass ratio to prepare a nutritional composition solution with a total concentration of 500 μg / mL by adding standard dilution water.

[0025] Embodiment 3 The embodiment provides a nutritional composition for improving muscle function, which is composed of OPO and OPL with a mass ratio of 45:45.

[0026] The OPO and the OPL are respectively prepared into 10.0 mg / mL mother liquor by using DMSO, and then are mixed according to the mass ratio to prepare a nutritional composition solution with a total concentration of 500 μg / mL by adding standard dilution water.

[0027] Embodiment 4 The embodiment provides a nutritional composition for improving muscle function, which is composed of OPO and OPL with a mass ratio of 40:48.

[0028] The OPO and the OPL are respectively prepared into 10.0 mg / mL mother liquor by using DMSO, and then are mixed according to the mass ratio to prepare a nutritional composition solution with a total concentration of 500 μg / mL by adding standard dilution water.

[0029] Embodiment 5 The embodiment provides a nutritional composition for improving muscle function, which is composed of IgG, OPO and OPL with a mass ratio of 15:60:24.

[0030] The IgG is prepared into 10.0 mg / mL mother liquor by using standard dilution water, and the OPO and the OPL are respectively prepared into 10.0 mg / mL mother liquor by using DMSO; then, the nutritional composition solution with a total concentration of 500 μg / mL is prepared by mixing according to the mass ratio and adding standard dilution water.

[0031] Embodiment 6 The embodiment provides a nutritional composition for improving muscle function, which is composed of IgG, OPO and OPL with a mass ratio of 15:45:36.

[0032] The IgG is prepared into 10.0 mg / mL mother liquor by using standard dilution water, and the OPO and the OPL are respectively prepared into 10.0 mg / mL mother liquor by using DMSO; then, the nutritional composition solution with a total concentration of 500 μg / mL is prepared by mixing according to the mass ratio and adding standard dilution water.

[0033] Embodiment 7 The embodiment provides a nutritional composition for improving muscle function, which is composed of IgG, OPO and OPL with a mass ratio of 15:45:45.

[0034] The IgG is prepared into a mother liquor with a concentration of 10.0 mg / mL by using standard dilution water, and the OPO and OPL are respectively prepared into mother liquors with a concentration of 10.0 mg / mL by using DMSO; after being mixed according to the mass ratio, standard dilution water is added to prepare a nutritional composition solution with a total concentration of 500 μg / mL, which is ready for use.

[0035] Example 8 The embodiment provides a nutritional composition for improving muscle function, which is composed of IgG, OPO and OPL with a mass ratio of 15:40:48.

[0036] The IgG is prepared into a mother liquor with a concentration of 10.0 mg / mL by using standard dilution water, and the OPO and OPL are respectively prepared into mother liquors with a concentration of 10.0 mg / mL by using DMSO; after being mixed according to the mass ratio, standard dilution water is added to prepare a nutritional composition solution with a total concentration of 500 μg / mL, which is ready for use.

[0037] Comparative Example 1 The comparative example provides a nutritional substance composed of IgG.

[0038] The IgG is prepared into a mother liquor with a concentration of 10.0 mg / mL by using standard dilution water, and standard dilution water is added to prepare a nutritional solution with a concentration of 500 μg / mL, which is ready for use.

[0039] Comparative Example 2 The comparative example provides a nutritional substance composed of OPO.

[0040] The OPO is prepared into a mother liquor with a concentration of 10.0 mg / mL by using DMSO, and standard dilution water is added to prepare a nutritional solution with a concentration of 500 μg / mL, which is ready for use.

[0041] Comparative Example 3 The comparative example provides a nutritional substance composed of OPL.

[0042] The OPL is prepared into a mother liquor with a concentration of 10.0 mg / mL by using DMSO, and standard dilution water is added to prepare a nutritional composition solution with a concentration of 500 μg / mL, which is ready for use.

[0043] Comparative Example 4 The comparative example provides a nutritional composition for improving muscle function, which is composed of IgG and OPO with a mass ratio of 15:60.

[0044] The IgG was prepared into a mother liquor of 10.0 mg / mL by using standard dilution water, and the OPO was prepared into a mother liquor of 10.0 mg / mL by using DMSO; after being mixed according to the mass ratio, standard dilution water was added to prepare a nutritional composition solution with a total concentration of 500 μg / mL, which was ready for use.

[0045] Comparative Example 5 This comparative example provides a nutritional composition for improving muscle function, which is composed of IgG and OPL in a mass ratio of 15:24.

[0046] The IgG was prepared into a mother liquor of 10.0 mg / mL by using standard dilution water, and the OPL was prepared into a mother liquor of 10.0 mg / mL by using DMSO; after being mixed according to the mass ratio, standard dilution water was added to prepare a nutritional composition solution with a total concentration of 500 μg / mL, which was ready for use.

[0047] Verification Test 1. Test Animals Wild-type AB strain zebrafish at 3 days after fertilization were raised in fish water at 28°C, and the water quality requirements were: 200 mg of instant sea salt was added to each 1 L of reverse osmosis water, the conductivity was 450 μS / cm~550 μS / cm; the pH was 6.5~8.5; the hardness was 50 mg / L CaCO3~100 mg / L CaCO3. The feeding management met the requirements of the International Assessment and Accreditation Committee for Experimental Animal Feeding Management.

[0048] 2. Evaluation of Muscle Function Improvement Zebrafish were randomly selected in a cell culture plate, 30 zebrafish were fed in each well, and the feeding system in each well was 3 mL. The normal control group, the model control group and each sample group (i.e. Example 1~8 groups and Comparative Example 1~5 groups) were set up. Except for the normal control group, the rest of the experimental groups were given 2 μmol / L of dexamethasone to establish a zebrafish sarcopenia model, and the sample groups were respectively given an equal amount of the nutritional liquid provided by Example 1~8 and Comparative Example 1~5. After 1 day of treatment at 28°C, 10 zebrafish were randomly selected from each group and transferred to a 96-well plate, 1 zebrafish per well, and the feeding system in each well was 200 μL. Then the movement distance of zebrafish within 1 h was measured by using a behavior analyzer, and the average value was taken to evaluate the improvement effect of different nutritional compositions on zebrafish sarcopenia. The test results are shown in Table 1 and Figure 1

[0049] 3. ATP Content Detection ​Zebrafish were randomly selected in cell culture plates, 30 zebrafish were fed in each well, and the feeding system in each well was 3 mL. The normal control group, the model control group and each sample group were set. Except for the normal control group, the rest of the experimental groups were given 2 μmol / L dexamethasone to establish a zebrafish myopathy model, and the sample groups were given an equal amount of nutritional liquid provided by Examples 1-8 and Comparative Examples 1-5. After 1 day of treatment at 28°C, the zebrafish in each group were collected, and the ATP content determination kit was used according to the operation, and the multifunctional enzyme label instrument was used to collect data, and the ATP content in each group of zebrafish was analyzed, and the average value was taken, and the test results are shown in Table 1 and Figure 2

[0050] Table 1 Test results of the moving distance and ATP content of zebrafish in each group

[0051] In the table, * indicates p<0.05 compared with the model control group, ** indicates p<0.01 compared with the model control group, and *** indicates p<0.001 compared with the model control group.

[0052] From Table 1 and Figure 1 It can be seen that compared with the normal control group, the moving distance of zebrafish in the model control group is reduced, indicating that the myopathy model is successfully established, and the movement ability of zebrafish is reduced. Compared with the model control group, the moving distance of zebrafish in Comparative Examples 1-3 is increased, but the difference is not significant, indicating that the use of IgG, OPO or OPL alone cannot improve the myopathy of zebrafish. From Comparative Examples 4-5 and Examples 1-4, it can be seen that the use of two components of IgG, OPO and OPL for compounding has an improvement effect on the moving distance of zebrafish, but the improvement effect of Comparative Examples 4-5 is not as good as OPO and OPL compounding (Examples 1-4), and with the decrease of the ratio of OPO and OPL, the improvement effect also increases. Compared with the model control group, the moving distance of zebrafish in Examples 5-8 is significantly increased (better than Examples 1-4), indicating that the compounding of IgG, OPO and OPL has a synergistic effect, and each component synergistically relieves myopathy and improves the movement ability of zebrafish.

[0053] From Table 1 and Figure 2 ​As can be seen, compared with the normal control group, the ATP content in the zebrafish in the model control group was significantly reduced, resulting in insufficient energy required for muscle contraction and decreased exercise capacity. Compared with the model control group, the ATP content in the zebrafish in the comparative examples 1-3 was increased, but the difference was not significant. The ATP content in the zebrafish in the comparative examples 4-5, the examples 1-4 and the examples 5-8 was significantly increased, and the effect was enhanced in turn; in particular, the effect was best when IgG, OPO and OPL were used in combination, and there was a synergistic effect. This shows that the nutritional composition provided by the application is helpful to improve muscle function by promoting energy metabolism.

[0054] The above merely describes preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement or improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A nutritional composition for improving muscle function, characterized in that, The nutritional composition comprises the following components in mass parts: 1,3-dioleic acid-2-palmitic acid triglyceride 40-60 parts and 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride 20-70 parts.

2. The nutritional composition for improving muscle function according to claim 1, wherein The mass ratio of the 1,3-dioleic acid-2-palmitic acid triglyceride and the 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride is 1:(0.4-1.8).

3. The nutritional composition for improving muscle function according to claim 2, wherein The mass ratio of the 1,3-dioleic acid-2-palmitic acid triglyceride and the 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride is 1:(0.5-1.5).

4. The nutritional composition for improving muscle function according to Claim 1, wherein The nutritional composition further comprises the following components in mass parts: immunoglobulin G 5-30 parts.

5. The nutritional composition for improving muscle function according to Claim 4, wherein The nutritional composition comprises the following components in mass parts: immunoglobulin G 10-20 parts, 1,3-dioleic acid-2-palmitic acid triglyceride 40-50 parts, and 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride 30-50 parts.

6. Use of the nutritional composition for improving muscle function according to any one of claims 1-5 in the preparation of a food.

7. Use of a nutritional composition for improving muscle function according to claim 6 for the manufacture of a food product, characterized in that, The food comprises at least one of a health food, a special medical purpose formula food, an infant formula food, or a special dietary food.

8. Use of a nutritional composition for improving muscle function according to claim 7, in the manufacture of a food product, characterized in that, The food comprises at least one of an infant formula milk powder, an infant complementary food, a children formula milk powder, a pregnant and lying-in woman milk powder, an old and middle-aged person milk powder, a liquid milk, a nutritional supplement, or a dietary supplement. The food comprises at least one of an infant formula milk powder, an infant complementary food, a children formula milk powder, a pregnant and lying-in woman milk powder, an old and middle-aged person milk powder, a liquid milk, a nutritional supplement, or a dietary supplement.

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