Detection method of portable blood potassium tester and portable blood potassium tester

Abstract

The invention discloses a detection method of a portable blood potassium tester and the portable blood potassium tester. The method comprises the steps that a communication mechanism is controlled to be selectively communicated with a cleaning liquid containing bottle, a peristaltic pump is controlled to conduct suction, and a first potential measurement value of an electrode detection mechanism is obtained; the communication mechanism is controlled to selectively communicate with the standard liquid containing bottle, the peristaltic pump is controlled to perform suction, and a second potential measurement value of the electrode detection mechanism is obtained; the communication mechanism is controlled to selectively communicate with the sampling bottle, the peristaltic pump is controlled to perform suction, and a third potential measurement value of the electrode detection mechanism is obtained; and determining the concentration of the target potassium ions in the liquid sample to be measured according to the three potential measurement values. According to the method, the detection result can be quickly obtained, the method can be suitable for scenes such as community medical centers and home detection, trace finger tip whole blood of the patient can be obtained during detection, operation is convenient, the patient can conduct self-detection or relatives assist detection, and therefore the medical cost of the patient is effectively reduced, and the convenience and compliance of blood potassium monitoring of the patient are improved.

Description

TECHNICAL FIELD

[0001] The embodiment of the present application relates to the technical field of medical devices, in particular to a detection method of a portable blood potassium detector and the portable blood potassium detector. BACKGROUND

[0002] Potassium ions play a key role in maintaining cell membrane potential, nerve conduction, muscle contraction and heart function. Abnormal blood potassium levels can interfere with myocardial cell electrical activity, such as hyperkalemia or hypokalemia, which can cause severe damage to heart function, respiratory function, kidney function and nervous system function. Therefore, it is crucial to detect blood potassium in time to correct its disorder, especially for some kidney patients, who may need to monitor blood potassium multiple times a week or even daily.

[0003] Currently, patients need to go to the hospital to detect blood potassium, which often requires high cost due to the particularity of the patient's condition (such as poor mobility, high frequency of medical treatment) and the limitation of the medical scene (such as complicated process, uneven distribution of resources). The blood potassium detection instruments provided by the hospital are usually electrolyte analyzers or biochemical analyzers / flow lines, which can provide several to dozens of test items including blood potassium to meet the needs of different patients, so the instruments are large in size, expensive in price, and require high supporting water, electricity equipment / facilities. In addition, the current detection sample is usually venous blood, which needs to be extracted by professional personnel and separated by centrifuge before being detected by the instrument, and at the same time, because the detection method is mostly batch detection, it usually takes 2-4 hours from blood extraction to result. It can be seen that the existing hospital blood potassium detection method is slow in detection efficiency and cannot be applied to community medical point (including drugstore) detection or home detection scene. SUMMARY

[0004] The embodiment of the present application provides a detection method of a portable blood potassium detector and the portable blood potassium detector to meet the needs of community medical point detection and home detection, improve the efficiency and convenience of blood potassium detection, and reduce the detection cost.

[0005] In a first aspect, the embodiment of the present application provides a detection method of a portable blood potassium detector, the portable blood potassium detector comprising an electrode detection mechanism, a communication mechanism and a peristaltic pump; the communication mechanism is used for selecting and communicating with a cleaning liquid containing bottle, a standard liquid containing bottle or a sampling bottle, the cleaning liquid containing bottle is used for storing cleaning liquid, the standard liquid containing bottle is used for storing standard liquid, and the sampling bottle is used for storing a liquid sample to be detected; the peristaltic pump is used for pumping the liquid in the target bottle communicated by the communication mechanism into the electrode detection mechanism for detection and then flowing out; the detection method comprises:

[0006] controlling the communicating mechanism to select communication with the cleaning solution containing bottle, controlling the peristaltic pump to perform pumping, and obtaining a first electrode potential measurement value of the electrode detection mechanism;

[0007] controlling the communicating mechanism to select communication with the standard solution containing bottle, controlling the peristaltic pump to perform pumping, and obtaining a second electrode potential measurement value of the electrode detection mechanism;

[0008] controlling the communicating mechanism to select communication with the sampling bottle, controlling the peristaltic pump to perform pumping, and obtaining a third electrode potential measurement value of the electrode detection mechanism;

[0009] determining a target potassium ion concentration in the liquid sample to be measured according to the first electrode potential measurement value, the second electrode potential measurement value, and the third electrode potential measurement value.

[0010] Optionally, the determining a target potassium ion concentration in the liquid sample to be measured according to the first electrode potential measurement value, the second electrode potential measurement value, and the third electrode potential measurement value comprises:

[0011] determining a quantitative relationship between electrode potential and potassium ion concentration according to the first electrode potential measurement value and the second electrode potential measurement value;

[0012] substituting the third electrode potential measurement value into the quantitative relationship to calculate the target potassium ion concentration.

[0013] Optionally, the determining a quantitative relationship between electrode potential and potassium ion concentration according to the first electrode potential measurement value and the second electrode potential measurement value comprises:

[0014] substituting the first electrode potential measurement value and a first preset potassium ion concentration of the cleaning solution into the Nernst equation to obtain a first relationship;

[0015] substituting the second electrode potential measurement value and a second preset potassium ion concentration of the standard solution into the Nernst equation to obtain a second relationship;

[0016] the Nernst equation is E = E0 + S * log 10 (C), wherein E represents electrode potential, C represents potassium ion concentration, E0 represents intercept, and S represents slope;

[0017] calculating the intercept and the slope according to the first relationship and the second relationship to obtain the quantitative relationship.

[0018] Optionally, the substituting the third electrode potential measurement value into the quantitative relationship to calculate the target potassium ion concentration comprises:

[0019] according to the formula E T = E0 + S * log10 (K + E = E T represents the third potential measurement value, K + represents the target potassium ion concentration, and M represents a preset dilution multiple.

[0020] Optionally, before the control of the communication mechanism to select the communication with the sampling bottle, the control of the peristaltic pump to perform the pumping, and the acquisition of the third potential measurement value of the electrode detection mechanism, the method further comprises:

[0021] The communication mechanism is controlled to select the communication with the cleaning liquid containing bottle, and the peristaltic pump is controlled to perform the pumping to clean the pipeline.

[0022] Optionally, after the control of the communication mechanism to select the communication with the cleaning liquid containing bottle, and the peristaltic pump to perform the pumping to clean the pipeline, the method further comprises:

[0023] The fourth potential measurement value of the electrode detection mechanism is acquired.

[0024] If the deviation between the fourth potential measurement value and the first potential measurement value exceeds a preset deviation threshold, the peristaltic pump is repeatedly controlled to perform the pumping until a new deviation is less than the preset deviation threshold.

[0025] Optionally, after the control of the communication mechanism to select the communication with the sampling bottle, the control of the peristaltic pump to perform the pumping, and the acquisition of the third potential measurement value of the electrode detection mechanism, the method further comprises:

[0026] The communication mechanism is controlled to select the communication with the cleaning liquid containing bottle, and the peristaltic pump is repeatedly controlled to perform the pumping.

[0027] Optionally, the control of the peristaltic pump to perform the pumping and the acquisition of the potential measurement value of the electrode detection mechanism comprises:

[0028] The peristaltic pump is controlled to perform the pumping to send the liquid in the target bottle into the electrode detection mechanism.

[0029] The potential measurement value of the electrode detection mechanism is acquired after a preset time length.

[0030] The peristaltic pump is controlled to perform the pumping to make the liquid in the electrode detection mechanism flow out.

[0031] Optionally, the electrode detection mechanism comprises a heating assembly; before the control of the communication mechanism to select the communication with the cleaning liquid containing bottle, the control of the peristaltic pump to perform the pumping, and the acquisition of the first potential measurement value of the electrode detection mechanism, the method further comprises:

[0032] controlling the heating assembly to perform constant temperature heating, so that the electrode detection mechanism works at a preset temperature.

[0033] In a second aspect, the embodiments of the present application further provide a portable blood potassium measuring instrument, which comprises a control module configured to execute the detection method of the portable blood potassium measuring instrument provided by any of the embodiments of the present application.

[0034] The embodiments of the present application provide a detection method of a portable blood potassium measuring instrument. First, the communication mechanism is controlled to select the communication with the cleaning liquid containing bottle, the peristaltic pump is controlled to perform suction, and the first potential measurement value of the electrode detection mechanism is obtained. Then, the communication mechanism is controlled to select the communication with the standard liquid containing bottle, the peristaltic pump is controlled to perform suction, and the second potential measurement value of the electrode detection mechanism is obtained. Then, the communication mechanism is controlled to select the communication with the sampling bottle, the peristaltic pump is controlled to perform suction, and the third potential measurement value of the electrode detection mechanism is obtained. Finally, the target potassium ion concentration in the liquid sample to be measured is determined according to the first potential measurement value, the second potential measurement value and the third potential measurement value. The detection method of the portable blood potassium measuring instrument provided by the embodiments of the present application can quickly obtain the detection result by detecting based on the portable blood potassium measuring instrument and calculating the required target potassium ion concentration through simple multiple potential measurements. The detection method can be applied to community medical point detection and home detection scenarios. During detection, a patient can take a small amount of finger tip peripheral blood without professional operations such as venous blood drawing and centrifugal separation of serum. The operation is convenient, and the patient can self-test or test with the assistance of relatives. Therefore, the cost of the patient for medical treatment is effectively reduced, and the convenience and compliance of the patient for monitoring blood potassium are improved. BRIEF DESCRIPTION OF DRAWINGS

[0035] Figure 1 A flowchart of the detection method of the portable blood potassium measuring instrument provided by the first embodiment of the present application;

[0036] Figure 2 A schematic diagram of the overall structure of the portable blood potassium measuring instrument provided by the first embodiment of the present application;

[0037] Figure 3 A schematic diagram of the local structure of the portable blood potassium measuring instrument provided by the first embodiment of the present application;

[0038] Figure 4 Another schematic diagram of the local structure of the portable blood potassium measuring instrument provided by the first embodiment of the present application;

[0039] Figure 5 Still another schematic diagram of the local structure of the portable blood potassium measuring instrument provided by the first embodiment of the present application;

[0040] Figure 6 Still another schematic diagram of the local structure of the portable blood potassium measuring instrument provided by the first embodiment of the present application;

[0041] Figure 7 The explosion view of the connecting mechanism of the portable blood potassium tester provided in the embodiment one of the present application is shown in the figure.

[0042] Figure 8 The explosion view of the electrode detection mechanism of the portable blood potassium tester provided in the embodiment one of the present application is shown in the figure. DETAILED DESCRIPTION

[0043] The present application will be further described below in conjunction with the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application. In addition, it should be noted that only the parts related to the present application are shown in the drawings for the convenience of description, but not all the structures.

[0044] Before discussing the example embodiments in more detail, it should be mentioned that some of the example embodiments are described as processes or methods depicted as flow charts. Although the steps of the processes are depicted in a sequential order, many of the steps can be performed in parallel, concurrently or simultaneously with one another. In addition, the order of the steps can be re-arranged. The processes can be terminated when their operations are completed, but can also have additional steps not included in the figure, which can also be performed after the operations of the processes are completed. The processes can correspond to methods, functions, procedures, subroutines, subprograms, etc.

[0045] Embodiment one

[0046] Figure 1 The flow chart of the detection method of the portable blood potassium tester provided in the embodiment one of the present application is shown in the figure. The present embodiment can be applicable to the case of quickly realizing blood potassium measurement in community medical point detection and home detection scenes. The method can be realized based on a portable blood potassium tester, which comprises an electrode detection mechanism, a connecting mechanism and a peristaltic pump. The connecting mechanism is used to select and communicate with a cleaning liquid containing bottle, a standard liquid containing bottle or a sampling bottle. The cleaning liquid containing bottle is used to store cleaning liquid, the standard liquid containing bottle is used to store standard liquid, and the sampling bottle is used to store liquid sample to be measured. The peristaltic pump is used to pump the liquid in the target bottle selected and communicated by the connecting mechanism into the electrode detection mechanism for detection and then flow out. As shown in the figure, the method specifically comprises the following steps: Figure 1

[0047] S11, control the connecting mechanism to select and communicate with the cleaning liquid containing bottle, control the peristaltic pump to pump, and obtain the first electric potential measurement value of the electrode detection mechanism.

[0048] S12, control the connecting mechanism to select and communicate with the standard liquid containing bottle, control the peristaltic pump to pump, and obtain the second electric potential measurement value of the electrode detection mechanism. ​

[0049] S13, control the communication mechanism to select communication with the sampling bottle, control the peristaltic pump to suck, and obtain the third potential measurement value of the electrode detection mechanism.

[0050] S14, determine the target potassium ion concentration in the liquid sample to be measured according to the first potential measurement value, the second potential measurement value and the third potential measurement value.

[0051] Specifically, for a portable blood potassium tester, such as Figures 2 to 8 , including a shell 1 with an inner cavity, a mounting frame 2 arranged in the shell 1, a communication mechanism 3 arranged on the mounting frame 2, an electrode detection mechanism 4, and a peristaltic pump 5; The shell 1 is provided with a cleaning liquid containing bottle 7 for storing cleaning liquid and a waste liquid containing bottle 8 for temporarily storing waste liquid, a first pipeline 12 is arranged in communication between the cleaning liquid containing bottle 7 and the communication mechanism 3, a second pipeline 13 is arranged in communication between the peristaltic pump 5 and the waste liquid containing bottle 8, the mounting frame 2 is provided with a first accommodation hole 21 for the first pipeline 12 to penetrate at the corresponding position of the communication mechanism 3, and the mounting frame 2 is provided with a second accommodation hole 22 for the second pipeline 13 to penetrate at the corresponding position of the peristaltic pump 5; The communication mechanism 3 and the electrode detection mechanism 4 are in communication with the first conveying pipe 15, the electrode detection mechanism 4 and the peristaltic pump 5 are in communication with the second conveying pipe 16, the peristaltic pump 5 includes a flexible hose 51 for connecting the second conveying pipe 16 and the second pipeline 13, a peristaltic roller 52 for extruding the flexible hose 51, and a driving motor 53 for driving the peristaltic roller 52 to rotate; The communication mechanism 3 is provided with a sampling tube 11 for communicating with a sampling bottle 6 temporarily storing the measured liquid; The mounting frame 2 can be provided with a clamping frame 24 for clamping the sampling tube 11, and the sampling tube 11 is stably arranged at a predetermined position on the shell 1 by the clamping frame 24. The shell 1 can also be provided with a standard liquid containing bottle 9 for storing standard liquid, and the standard liquid containing bottle 9 and the communication mechanism 3 are in communication with the third pipeline 14, and the third pipeline 14 penetrates the first accommodation hole 21.

[0052] The electrode detection mechanism 4 adopts ion selective electrode method to detect blood potassium, uses specific electrodes (potassium ion selective electrode and reference electrode) to produce selective response to potassium ions in the measured liquid, measures electrode potential to determine potassium ion concentration. Specifically, it can include an electrode detection component 41 for detecting the measured liquid, a heating component 42 for heating the electrode detection component 41, and a liquid level sensor 43 for monitoring the liquid level of the solution in the second delivery pipe 16. The electrode detection component 41 is heated by the heating component 42 so that it can perform detection work within a predetermined environmental temperature, which helps to ensure the accuracy and precision of the detection results. By providing the liquid level sensor 43 on the second delivery pipe 16 to monitor the liquid level of the solution in the second delivery pipe 16, the real-time liquid level in the second delivery pipe 16 can be monitored in real time, and the real-time liquid level of the electrode detection component 41 can be monitored in real time, ensuring that the measured liquid has been delivered to the electrode detection component 41 for detection. The heating component 42 can include a heating sheet 421 for heating the electrode detection component 41, and a temperature sensor 422 for monitoring the temperature of the electrode detection component 41. The electrode detection component 41 is heated to a predetermined temperature by the heating sheet 421, and the temperature of the electrode detection component 41 is monitored in real time by the temperature sensor 422. When the electrode detection component 41 reaches the predetermined temperature, heating is stopped in time. When the electrode detection component 41 is below the predetermined temperature, the heating sheet 421 is started to heat in time, ensuring that the electrode detection component 41 is constantly at the predetermined temperature for detection, which helps to ensure the accuracy and precision of the detection results.

[0053] The communication mechanism 3 can include a telescopic motor 31 and a valve 32 fixedly connected with the telescopic motor 31, and the mounting frame 2 is provided with a fixing frame 23 for fixedly connecting the telescopic motor 31, and the communication mechanism 3 is stably mounted on the mounting frame 2 through the fixing frame 23, and the valve 32 is driven to move up and down relative to the mounting frame 2 by the telescopic motor 31, so as to realize the communication of the valve 32 with the first pipeline 12, the third pipeline 14 and the sampling pipe 11 respectively, and realize the delivery of different solutions to the electrode detection mechanism 4. The valve 32 includes a valve core 321 fixedly connected with the telescopic motor 31 and a valve body 323 fixedly connected with the fixing frame 23, the valve core 321 is provided with a communication hole 322, the valve body 323 is provided with a penetrating hole 324 for the valve core 321 to penetrate, and the valve body 323 is in communication with the first pipeline 12, the third pipeline 14 and the sampling pipe 11 respectively, and the valve core 321 is driven to move up and down in the penetrating hole 324 of the valve body 323 by the telescopic motor 31, so as to realize that the communication hole 322 of the valve core 321 can be in communication with the first pipeline 12, the third pipeline 14 and the sampling pipe 11 at different height positions respectively, so as to realize that the first pipeline 12, the third pipeline 14 and the sampling pipe 11 can be in communication with the first delivery pipe 15 respectively when the valve core 321 is at different height positions, and realize the delivery of the required solution to the electrode detection mechanism 4. The fixing frame 23 can be provided with a first position sensor 33 for monitoring the position of the valve core 321, for real-time monitoring of the height position of the valve core 321, so as to control the start and stop and telescopic stroke of the telescopic motor 31, and realize the delivery of the required solution to the electrode detection mechanism 4.

[0054] The peristaltic pump 5 drives the peristaltic roller 52 to rotate through the driving motor 53, and when the peristaltic roller 52 rolls on the surface of the flexible hose 51, it successively extrudes the surface of the flexible hose 51, so that the extruded part forms a sealed space, and the internal fluid is pushed forward. After the peristaltic roller 52 leaves, the flexible hose 51 restores to its original state due to its own elasticity, forming a local vacuum to suck in the subsequent fluid, thereby realizing continuous delivery, and the advantage is that the fluid only contacts the flexible hose 51, avoiding pollution of the fluid by other parts of the peristaltic pump 5. The mounting frame 2 can be provided with a support frame 25 for mounting the peristaltic pump 5, which realizes stable mounting of the peristaltic pump 5 at a predetermined position on the mounting frame 2, and the support frame 25 is provided with a second position sensor 54 for monitoring the position of the peristaltic roller 52, which realizes real-time monitoring of the rotation angle position of the peristaltic roller 52 through the second position sensor 54, and realizes real-time monitoring of the delivery progress of the peristaltic pump 5 to the fluid.

[0055] The portable blood potassium detector can further include a control module 17 and a touch screen 18 electrically connected with the control module 17, the communication mechanism 3, the electrode detection mechanism 4 and the peristaltic pump 5 are electrically connected with the control module 17, the start and stop and the operation mode of the control module 17 can be controlled through the touch screen 18, the operation state of the communication mechanism 3, the electrode detection mechanism 4 and the peristaltic pump 5 is controlled by the control module 17, and the corresponding data is read and presented to the user for checking through the touch screen 18; meanwhile, the first mounting hole for mounting the touch screen 18 is provided through the shell 1, the touch screen 18 is stably mounted at the preset position of the shell 1, which is convenient for the user to read the detection data and control the portable blood potassium detection device.

[0056] The portable blood potassium detector can further include a control module 17 and a touch screen 18 electrically connected with the control module 17, the communication mechanism 3, the electrode detection mechanism 4 and the peristaltic pump 5 are electrically connected with the control module 17, the start and stop and the operation mode of the control module 17 can be controlled through the touch screen 18, the operation state of the communication mechanism 3, the electrode detection mechanism 4 and the peristaltic pump 5 is controlled by the control module 17, and the corresponding data is read and presented to the user for checking through the touch screen 18; meanwhile, the first mounting hole for mounting the touch screen 18 is provided through the shell 1, the touch screen 18 is stably mounted at the preset position of the shell 1, which is convenient for the user to read the detection data and control the portable blood potassium detection device.

[0057] In addition, a disposable peripheral blood collection kit can also be correspondingly configured, including disposable skin disinfecting supplies, disposable spring blood lancets, disposable micro pipettes, disposable sampling bottles, disposable waste boxes (containing detoxification liquid) and the like, to prevent cross infection. The waste liquid containing bottle 8 can also be a disposable waste liquid collection bottle, which stores disinfectant liquid and can disinfect the waste liquid generated during operation for harmless treatment to prevent biological hazards.

[0058] On the basis of the above structure, each time detection is needed, a small amount (50ul) of peripheral whole blood of the measured object can be taken and placed in the sampling bottle and connected to the designated position of the detector, and detection is started. After starting detection, the potential measurement values of the washing liquid, the standard liquid and the liquid sample to be measured can be measured respectively for calculating the target potassium ion concentration. First, low concentration calibration can be performed, the communication mechanism is controlled to select communication with the washing liquid containing bottle, the peristaltic pump is controlled to perform suction, and the first potential measurement value of the electrode detection mechanism is obtained. Then high concentration calibration can be performed, the communication mechanism is controlled to select communication with the standard liquid containing bottle, the peristaltic pump is controlled to perform suction, and the second potential measurement value of the electrode detection mechanism is obtained. The liquid sample to be measured is measured, the communication mechanism is controlled to select communication with the sampling bottle, the peristaltic pump is controlled to perform suction, and the third potential measurement value of the electrode detection mechanism is obtained. Through the above measurement process, the blood potassium detection result can be obtained within five minutes.

[0059] Optionally, the control of the peristaltic pump to perform the pumping and the acquisition of the potential measurement value of the electrode detection mechanism comprises: controlling the peristaltic pump to perform the pumping to send the liquid in the target bottle into the electrode detection mechanism; waiting for a preset time length to acquire the potential measurement value of the electrode detection mechanism; and controlling the peristaltic pump to perform the pumping to make the liquid in the electrode detection mechanism flow out.

[0060] Specifically, for low concentration calibration, the communication mechanism is controlled to communicate the first pipeline and the first delivery pipe, and then the driving motor of the peristaltic pump is controlled to drive the peristaltic roller to rotate, so that the peristaltic roller extrudes the flexible hose to form a local vacuum, thereby pumping the cleaning liquid in the cleaning liquid containing bottle, and the cleaning liquid flows through the first pipeline, the communication mechanism, the first delivery pipe in sequence and is delivered to the electrode detection mechanism for detection. Then a preset time length (e.g. 5 seconds) can be waited for to balance, and the first potential measurement value of the electrode detection mechanism at this time is acquired. Subsequently, the peristaltic pump is controlled to pump the cleaning liquid to flow out of the electrode detection mechanism, and can flow through the second delivery pipe, the flexible hose, the second pipeline in sequence, and finally be delivered to the waste liquid containing bottle for temporary storage. Similarly, for high concentration calibration, the communication mechanism is controlled to communicate the third pipeline and the first delivery pipe, and the peristaltic pump is controlled to start operation to pump the standard liquid in the standard liquid containing bottle, and the standard liquid flows through the third pipeline, the communication mechanism, the first delivery pipe in sequence and is delivered to the electrode detection mechanism for detection. Then a preset time length (e.g. 5 seconds) can be waited for to balance, and the second potential measurement value of the electrode detection mechanism at this time is acquired. Subsequently, the peristaltic pump is controlled to pump the standard liquid to flow out of the electrode detection mechanism, and flows through the second delivery pipe, the flexible hose, the second pipeline in sequence, and finally is delivered to the waste liquid containing bottle for temporary storage. For the measurement of the liquid sample to be measured, the communication mechanism is controlled to communicate the sampling pipe and the first delivery pipe, and the peristaltic pump is controlled to start operation to pump the liquid sample to be measured in the sampling bottle, and the liquid sample to be measured flows through the sampling pipe, the communication mechanism, the first delivery pipe in sequence and is delivered to the electrode detection mechanism for detection. Then a preset time length (e.g. 5 seconds) can be waited for to balance, and the third potential measurement value of the electrode detection mechanism at this time is acquired. Subsequently, the peristaltic pump is controlled to pump the liquid sample to be measured to flow out of the electrode detection mechanism, and flows through the second delivery pipe, the flexible hose, the second pipeline in sequence, and finally is delivered to the waste liquid containing bottle for temporary storage.

[0061] In an optional embodiment, the determination of the target potassium ion concentration in the liquid sample to be measured according to the first potential measurement value, the second potential measurement value and the third potential measurement value comprises: determining a quantitative relationship between the electrode potential and the potassium ion concentration according to the first potential measurement value and the second potential measurement value; and substituting the third potential measurement value into the quantitative relationship to calculate the target potassium ion concentration.

[0062] Further optionally, the determining the quantitative relationship between the electrode potential and the potassium ion concentration according to the first potential measurement value and the second potential measurement value comprises: substituting the first potential measurement value and the first preset potassium ion concentration of the cleaning solution into the Nernst equation to obtain a first relationship; substituting the second potential measurement value and the second preset potassium ion concentration of the standard solution into the Nernst equation to obtain a second relationship; the Nernst equation is: E = E0 + S*log 10 (C), wherein E represents the electrode potential, C represents the potassium ion concentration, E0 represents the intercept, and S represents the slope; and calculating the intercept and the slope according to the first relationship and the second relationship to obtain the quantitative relationship.

[0063] Specifically, substituting the data of the cleaning solution and the standard solution into the Nernst equation to obtain a first relationship E1 = E0 + S*log 10 (C1) and a second relationship E2 = E0 + S*log 10 (C2), wherein E1 represents the first potential measurement value, C1 represents the first preset potassium ion concentration, E2 represents the second potential measurement value, and C2 represents the second preset potassium ion concentration. Subtracting the two equations to obtain E1-E2 = S*[log 10 (C1) - log 10 (C2)], so that the slope formula S = (E1-E2) ÷ [log 10 (C1) - log 10 (C2)] = (E1-E2) ÷ log 10 [(C1) ÷ (C2)] can be obtained. Then substituting the calculated slope S into the first relationship or the second relationship, the intercept E0 can be obtained, such as E0 = E1-S*log 10 (C1) for the first relationship. For example, the cleaning solution C1 = 0.8 mmol / L, E1 = 2.0 mV, the standard solution C2 = 2.0 mmol / L, E2 = 20.0 mV, the calculated slope S = (2.0-20.0) ÷ log 10 (0.8 / 2.0) = 45.23, and the calculated intercept E0 = 2.0-45.23*log 10 (0.8) = 6.383.

[0064] Further optionally, the substituting the third potential measurement value into the quantitative relationship to calculate the target potassium ion concentration comprises: calculating the target potassium ion concentration according to the formula E T = E0 + S*log 10 (K + ÷ M), wherein E T represents the third potential measurement value, K +represents the target potassium ion concentration, and M represents a preset dilution multiple.

[0065] Specifically, after the target object peripheral whole blood is taken, a preset dilution multiple dilution can be performed in the sampling bottle, so as to suck the to-be-measured liquid sample into the electrode detection mechanism for detection. For the concentration calculation formula, the logarithmic form log 10 (K + ÷M)=(E T -E0)÷S, and the concentration (K + )=10 [(ET-E0)÷S] ×M is obtained through the anti-logarithmic operation. As exemplified above, assuming that the third potential measurement value of the to-be-measured liquid sample diluted by 4 times is 15.0 mV, the target potassium ion concentration (K + )T=10 [(15-6.383)÷45.23] ×4=6.20 mmol / L can be calculated based on the above formula.

[0066] On the basis of the above technical solution, before the control of the communication mechanism to select the communication with the sampling bottle, the control of the peristaltic pump to perform the suction, and the acquisition of the third potential measurement value of the electrode detection mechanism, the technical solution further comprises: controlling the communication mechanism to select the communication with the cleaning liquid containing bottle, and controlling the peristaltic pump to perform the suction, so as to clean the pipeline.

[0067] Specifically, after the high-concentration calibration is performed, in order to avoid the influence of the residual standard liquid in the pipeline on the measurement result of the to-be-measured liquid sample, the pipeline can be cleaned before the detection, so as to remove the residual standard liquid in the pipeline. The communication mechanism can be controlled to communicate with the first pipeline and the first conveying pipe, and the peristaltic pump can be controlled to start the operation, so as to suck the cleaning liquid in the cleaning liquid containing bottle, and the cleaning liquid is conveyed to the electrode detection mechanism for cleaning. Then, the peristaltic pump can be controlled to suck the cleaning liquid out of the electrode detection mechanism and convey the cleaning liquid to the waste liquid containing bottle for temporary storage after a preset time period (for example, 5 seconds). The cleaning process can be repeated multiple times to ensure the cleaning effect.

[0068] Further, after the control of the communication mechanism to select the communication with the cleaning liquid containing bottle, and the control of the peristaltic pump to perform the suction, so as to clean the pipeline, the technical solution further comprises: acquiring a fourth potential measurement value of the electrode detection mechanism; and if the deviation between the fourth potential measurement value and the first potential measurement value exceeds a preset deviation threshold, repeatedly controlling the peristaltic pump to perform the suction until the new deviation is less than the preset deviation threshold.

[0069] Specifically, the fourth potential measurement value detected by the electrode detection mechanism can be obtained after the cleaning liquid is delivered to the electrode detection mechanism and after a preset time period elapses, so as to ensure that the measurement value accurately reflects the current liquid condition in the pipeline. Then, the fourth potential measurement value can be compared with the first potential measurement value obtained by the low-concentration calibration. If the deviation between the two values exceeds a preset deviation threshold (e.g., 2.5%), it can be considered that the cleaning is not complete, and the cleaning and potential comparison process can be performed again until the deviation is less than the preset deviation threshold.

[0070] On the basis of the above technical solution, after the control of the communication mechanism to select the communication with the sampling bottle, the control of the peristaltic pump to perform the suction, and the acquisition of the third potential measurement value of the electrode detection mechanism, the method further includes: controlling the communication mechanism to select the communication with the cleaning liquid containing bottle, and repeatedly controlling the peristaltic pump to perform the suction.

[0071] Specifically, after the measurement of the to-be-measured liquid sample is completed, the pipeline can be cleaned multiple times to remove the residual to-be-measured liquid sample in the pipeline, so as to ensure the accuracy of the result of the next detection of a new to-be-measured liquid sample. After the third potential measurement value is obtained, the communication mechanism can be controlled to communicate with the first pipeline and the first delivery pipe, and the peristaltic pump can be controlled to start the operation to perform the suction of the cleaning liquid in the cleaning liquid containing bottle. The cleaning liquid flows through the first pipeline, the communication mechanism, the first delivery pipe, and the electrode detection mechanism in sequence and is delivered to the electrode detection mechanism for cleaning. Then, the peristaltic pump can be controlled to suction the cleaning liquid to flow out of the electrode detection mechanism and to flow through the second delivery pipe, the flexible hose, and the second pipeline in sequence and finally to be delivered to the waste liquid containing bottle for temporary storage. The operation of the peristaltic pump can be repeated multiple times (e.g., no less than three times) to repeatedly clean the pipeline.

[0072] On the basis of the above technical solution, the electrode detection mechanism includes a heating assembly. Before the control of the communication mechanism to select the communication with the cleaning liquid containing bottle, the control of the peristaltic pump to perform the suction, and the acquisition of the first potential measurement value of the electrode detection mechanism, the method further includes: controlling the heating assembly to perform constant temperature heating, so that the electrode detection mechanism works at a preset temperature.

[0073] Specifically, at the beginning of each detection, the heating assembly can first be started to heat the electrode detection assembly, and the temperature of the electrode detection assembly can be monitored in real time by the temperature sensor, so that the electrode detection assembly can be kept at a preset temperature (preferably 37 degrees Celsius), and then the electrode detection assembly can be kept at the preset temperature for detection, thereby improving the accuracy and detection precision of the detection result. In addition, before calibration, the first position sensor and the second position sensor can be used to initialize the positions of the valve core and the peristaltic roller to the preset initial positions. After the potential measurement of the to-be-measured liquid sample is completed, specifically, after the residual to-be-measured liquid sample is removed through multiple cleanings, the valve core and the peristaltic roller are adjusted back to the preset initial positions through the first position sensor and the second position sensor again.

[0074] For each detection process of the potassium concentration in blood, the above low-concentration calibration and high-concentration calibration processes can be performed again, and the target potassium ion concentration can be calculated in combination with the potential measurement value of the current to-be-measured liquid sample, thereby ensuring the accuracy and detection precision of the result. Further preferably, each detection process can sequentially include the above constant-temperature heating, low-concentration calibration, high-concentration calibration, cleaning of residual standard liquid, measurement of the to-be-measured liquid sample, and cleaning of residual to-be-measured liquid sample.

[0075] The technical solution provided by the embodiment of the present application first controls the communication mechanism to select communication with the cleaning liquid containing bottle, controls the peristaltic pump to perform suction, and obtains a first potential measurement value of the electrode detection mechanism, then controls the communication mechanism to select communication with the standard liquid containing bottle, controls the peristaltic pump to perform suction, and obtains a second potential measurement value of the electrode detection mechanism, and then controls the communication mechanism to select communication with the sampling bottle, controls the peristaltic pump to perform suction, and obtains a third potential measurement value of the electrode detection mechanism, so as to determine the target potassium ion concentration in the to-be-measured liquid sample according to the obtained first potential measurement value, second potential measurement value, and third potential measurement value. By detecting based on the portable blood potassium detector and calculating the required target potassium ion concentration through simple multiple potential measurements, the detection result can be quickly obtained, and the device can be applied to community medical point detection and home detection scenes. During detection, a small amount of finger tip peripheral blood of a patient can be taken, without the need for professional operations such as venous blood drawing and centrifugal separation of serum, and the operation is convenient. The patient can self-test or a relative can assist in detection, thereby effectively reducing the medical cost of the patient and improving the convenience and compliance of the patient in monitoring blood potassium.

[0076] Embodiment two

[0077] The second embodiment of the present application provides a portable blood potassium detector, which comprises a control module, and the control module is used to execute the detection method of the portable blood potassium detector provided by any of the embodiments of the present application, and has the beneficial effects corresponding to the execution method. Correspondingly, the detector also comprises an electrode detection mechanism, a communication mechanism and a peristaltic pump, so as to control and collect data of the electrode detection mechanism, the communication mechanism and the peristaltic pump through the control module, thereby realizing blood potassium detection. Further, the specific structure of the portable blood potassium detector provided by the present embodiment can refer to the description in the above embodiments, which will not be repeated here.

[0078] It should be noted that the above are only the preferred embodiments of the present application and the technical principles applied. Those skilled in the art will understand that the present application is not limited to the specific embodiments described herein, and those skilled in the art can make various obvious changes, re-adjustments and substitutions without departing from the scope of the present application. Therefore, although the present application has been described in more detail through the above embodiments, the present application is not limited to the above embodiments, and can include more other equivalent embodiments without departing from the concept of the present application, and the scope of the present application is determined by the scope of the appended claims.

Claims

1. A detection method using a portable serum potassium analyzer, characterized in that, The portable blood potassium analyzer includes an electrode detection mechanism, a communication mechanism, and a peristaltic pump; the communication mechanism is used to selectively communicate with a cleaning solution container, a standard solution container, or a sampling bottle; the cleaning solution container is used to store cleaning solution, the standard solution container is used to store standard solution, and the sampling bottle is used to store the liquid sample to be tested. The peristaltic pump is used to draw liquid from the target bottle selected and connected by the communication mechanism, send it to the electrode detection mechanism for detection, and then allow it to flow out; the detection method includes: The communication mechanism is controlled to connect with the cleaning fluid container, the peristaltic pump is controlled to perform suction, and the first potential measurement value of the electrode detection mechanism is obtained. The communication mechanism is controlled to connect with the standard solution container, the peristaltic pump is controlled to perform suction, and the second potential measurement value of the electrode detection mechanism is obtained. The communication mechanism is controlled to connect with the sampling bottle, the peristaltic pump is controlled to perform suction, and the third potential measurement value of the electrode detection mechanism is obtained; The target potassium ion concentration in the liquid sample to be tested is determined based on the first potential measurement value, the second potential measurement value, and the third potential measurement value.

2. The detection method of the portable serum potassium analyzer according to claim 1, characterized in that, Determining the target potassium ion concentration in the liquid sample to be tested based on the first potential measurement value, the second potential measurement value, and the third potential measurement value includes: The quantitative relationship between electrode potential and potassium ion concentration is determined based on the first potential measurement value and the second potential measurement value; Substitute the measured value of the third potential into the quantitative relationship to calculate the target potassium ion concentration.

3. The detection method of the portable serum potassium analyzer according to claim 2, characterized in that, Determining the quantitative relationship between electrode potential and potassium ion concentration based on the first potential measurement value and the second potential measurement value includes: Substituting the first potential measurement value and the first preset potassium ion concentration of the cleaning solution into the Nernst equation, we obtain the first relationship; Substituting the second potential measurement value and the second preset potassium ion concentration of the standard solution into the Nernst equation, we obtain the second relationship. The Nernst equation is: E = E0 + S * log 10 (C), where E represents the electrode potential, C represents the potassium ion concentration, E0 represents the intercept, and S represents the slope; The intercept and the slope are calculated based on the first and second relations to obtain the quantitative relationship.

4. The detection method of the portable serum potassium analyzer according to claim 3, characterized in that, The step of substituting the measured value of the third potential into the quantitative relationship to calculate the target potassium ion concentration includes: According to formula E T =E0+S*log 10 (K + ÷M) calculate the target potassium ion concentration, where E T K represents the measured value of the third potential. + The target potassium ion concentration is represented by M, where M represents the preset dilution factor.

5. The detection method of the portable serum potassium analyzer according to claim 1, characterized in that, Before controlling the communication mechanism to connect with the sampling bottle, controlling the peristaltic pump to perform suction, and obtaining the third potential measurement value of the electrode detection mechanism, the method further includes: The control mechanism is configured to connect with the cleaning fluid container and the peristaltic pump is configured to perform suction to clean the pipeline.

6. The detection method of the portable serum potassium analyzer according to claim 5, characterized in that, After controlling the communication mechanism to connect with the cleaning fluid container and controlling the peristaltic pump to perform suction to clean the pipeline, the method further includes: Obtain the fourth potential measurement value of the electrode detection mechanism; If the deviation between the fourth potential measurement value and the first potential measurement value exceeds a preset deviation threshold, the peristaltic pump is repeatedly controlled to perform suction until the new deviation is less than the preset deviation threshold.

7. The detection method of the portable serum potassium analyzer according to claim 1, characterized in that, After controlling the communication mechanism to connect with the sampling bottle, controlling the peristaltic pump to perform suction, and acquiring the third potential measurement value of the electrode detection mechanism, the method further includes: The communication mechanism is controlled to connect with the cleaning fluid container, and the peristaltic pump is repeatedly controlled to perform suction.

8. The detection method of the portable serum potassium analyzer according to claim 1, characterized in that, Controlling the peristaltic pump to perform suction and acquiring the potential measurement value of the electrode detection mechanism includes: The peristaltic pump is controlled to perform suction to deliver the liquid in the target bottle into the electrode detection mechanism; Wait for a preset time to obtain the potential measurement value of the electrode detection mechanism; The peristaltic pump is controlled to draw in liquid so that the liquid in the electrode detection mechanism flows out.

9. The detection method of the portable serum potassium analyzer according to claim 1, characterized in that, The electrode detection mechanism includes a heating component; before controlling the communication mechanism to connect with the cleaning fluid container, controlling the peristaltic pump to perform suction, and acquiring the first potential measurement value of the electrode detection mechanism, it further includes: The heating component is controlled to perform constant temperature heating so that the electrode detection mechanism operates at a preset temperature.

10. A portable serum potassium analyzer, characterized in that, The instrument includes a control module for performing the detection method of the portable blood potassium analyzer as described in any one of claims 1-9.

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