Tea-pine composition with cell repairing effect as well as preparation method and application of tea-pine composition

Abstract

The invention relates to the technical field of cosmetics, in particular to a tea-pine composition with a cell repairing effect and a preparation method and application thereof. The tea-pine composition with the cell repairing effect is prepared from a Longjing green tea extract and a pine plant extract, the mass ratio of the Longjing green tea extract to the pine plant extract is (2-5): (1.5-5). By compounding the Longjing green tea extract and the pine plant extract, the limitation of a single component can be reduced, multiple antioxidation can be realized, the antioxidation effect is more stable and more lasting, and cell repair is remarkably improved, so that the problems of inflammation and aging of skin are solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetic technology, and in particular to a tea-pine composition with cell repair efficacy, and a preparation method and application thereof. BACKGROUND

[0002] According to relevant reports, the global market size of pine bark extract is expected to grow significantly by 2025, and daily chemicals are an important application field, and the demand for daily chemicals has a clear trend of annual compound growth rate. Pine plant extracts contain multiple skin care active ingredients. Pine bark contains "pinosyl", which has strong antioxidant activity and can inhibit skin aging and fade color spots. Its anti-inflammatory and antibacterial properties can reduce skin sensitivity and inflammation. Pine oil contains polyphenols and other antioxidant ingredients, which can neutralize free radicals, and also has anti-inflammatory and antibacterial properties, which can help soothe inflamed skin and maintain skin luster due to good penetration. In addition, larch bark procyanidins can whiten, moisturize, inhibit melanin production, and even prevent ultraviolet rays.

[0003] At present, consumers increasingly prefer cosmetics containing natural ingredients and expect to reduce contact with chemical synthetic substances. Pine plant extracts are derived from nature and meet the "natural and healthy" consumer preference. In the future, pine plant ingredients have great potential for exploration in the skin care field and are favored by consumers. They can help develop alternative ingredients such as natural preservatives to meet the market demand for safer and milder cosmetics. SUMMARY

[0004] The present application aims to overcome the shortcomings of the prior art and provide a tea-pine composition with cell repair efficacy, which significantly improves the whitening and wrinkle-fading effects of cosmetics through the synergistic effect of the components.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the present application is as follows:

[0006] In a first aspect, the present application provides a tea-pine composition with cell repair efficacy, comprising Longjing green tea extract and pine plant extract; the mass ratio of the Longjing green tea extract and the pine plant extract is Longjing green tea extract:pine plant extract=(2-5):(1.5-5).

[0007] West Lake Longjing is one of the top ten famous teas in China, belonging to green tea, with the reputation of "Paradise Gem" and "Green Tea Queen". According to GB / T 18650-2008, Longjing tea produced in Hangzhou West Lake District is called West Lake Longjing tea, about 168 square kilometers. It is rich in tea polyphenols, mainly catechins, vitamin C / E, caffeine, amino acids, and has the effects of anti-oxidation, anti-aging, and auxiliary whitening. Pine plant ingredients have great potential in the field of skin care. The present application combines Longjing green tea extract and pine plant extract to develop a tea-pine composition with cell repair function, which can reduce the limitations of single components, achieve "multiple antioxidant", make the antioxidant effect more stable and persistent, and significantly improve cell repair, thereby improving skin inflammation and aging problems.

[0008] Preferably, the mass ratio of the Longjing green tea extract and the pine plant extract is Longjing green tea extract: pine plant extract = (3-5) :(1.5-5).

[0009] Preferably, the pine plant extract includes Gan Song root extract and Jin Song root extract.

[0010] Gan Song root extract is an important traditional Chinese medicine, containing volatile oil, valerenol, valerian ketone, flavonoids, triterpenoids, etc., which can inhibit nerve sensitive signals, relieve skin burning and itching, and is especially friendly to sensitive skin with damaged barrier. It can also reduce the release of inflammatory mediators, while promoting the proliferation of keratinocytes and accelerating skin barrier repair.

[0011] Jin Song is originally from Japan and is now planted in various parts of China. Jin Song root is rich in terpenoids, including the iconic Jin Song biflavone and hinokitiol, which have strong antioxidant capacity and can resist multiple exogenous damage such as ultraviolet and blue light, protecting cells from oxidative stress. However, the clinical data and mechanism research of its skin care effect are not as sufficient as other plants, and some of its effects rely on traditional application experience.

[0012] The present application combines Longjing green tea extract, Gan Song root extract and Jin Song root extract, and the three are compounded, with synergistic effect of antioxidant and anti-inflammatory, resisting external damage, regulating and repairing cell damage, delaying aging, and relieving sensitive discomfort. The adaptation of natural ingredients of the three meets the multi-dimensional needs of skin care, is suitable for various skin types, especially for dull and sensitive skin, and realizes the maximization of skin care efficacy.

[0013] Preferably, the mass ratio of the Gan Song root extract and the Jin Song root extract is Gan Song root extract: Jin Song root extract = (1-3) :(0.5-2).

[0014] Preferably, the tea-pine composition with cell repair efficacy comprises the following components in parts by weight: 3-5 parts of Longjing green tea extract, 1-3 parts of Gan Song root extract, and 0.3-2 parts of Jin Song root extract.

[0015] More preferably, the tea-pine composition with cell repair efficacy comprises the following components in parts by weight: 5 parts of Longjing green tea extract, 3 parts of Gan Song root extract, and 2 parts of Jin Song root extract.

[0016] The present application has found through experiments that when the three components are matched in the optimal amount defined above, the respective functions can be maximized, thereby ensuring that the tea-pine composition prepared has excellent effects in cell repair and anti-inflammatory and anti-aging, and has the best cost performance.

[0017] In a second aspect, the present application provides use of the tea-pine composition with cell repair efficacy described above in the preparation of a cosmetic product.

[0018] In a third aspect, the present application provides a cosmetic product with cell repair efficacy, which comprises the tea-pine composition with cell repair efficacy described above and an auxiliary material; the auxiliary material comprises at least one of a humectant, a neutralizing agent, a thickening agent, a preservative, and water.

[0019] Preferably, the cosmetic product comprises the following components in mass percentage: 3.5-10% of the tea-pine composition with cell repair efficacy, 9.6-16.3% of the humectant, 0.1-0.4% of the neutralizing agent, 0.2-0.4% of the thickening agent, 0.9-1.2% of the preservative, and water in the remainder.

[0020] Preferably, the humectant comprises at least one of glycerol, 1,3-propanediol, panthenol, sodium hyaluronate, and tocopheryl acetate.

[0021] Preferably, in the humectant, the mass ratio of glycerol, 1,3-propanediol, panthenol, sodium hyaluronate, and tocopheryl acetate is glycerol: 1,3-propanediol: panthenol: sodium hyaluronate: tocopheryl acetate = (5-8):(3-5):(1-2):(0.1-0.3):(0.5-1).

[0022] Preferably, the neutralizing agent comprises triethanolamine.

[0023] Preferably, the thickening agent comprises at least one of xanthan gum and carbomer.

[0024] Preferably, in the thickening agent, the mass ratio of xanthan gum and carbomer is xanthan gum: carbomer = (0.1-0.2):(0.1-0.2).

[0025] Preferably, the preservative comprises at least one of phenoxyethanol and ethylhexylglycerin.

[0026] Preferably, in the preservative, the mass ratio of phenoxyethanol and ethylhexylglycerin is phenoxyethanol: ethylhexylglycerin = (0.8-1):(0.1-0.2).

[0027] Preferably, the dosage form of the cosmetic includes water, milk, and cream.

[0028] In a fourth aspect, the present application provides a preparation method of the above-mentioned cosmetic with cell repair effect, comprising the following steps:

[0029] S1, sieving the pine plant extract, and then mixing it with part of water to obtain a pine plant extract solution;

[0030] S2, adding 8.1-13.3% of a humectant and a thickening agent to the remaining water, stirring uniformly, heating, and keeping warm to obtain a mixture a;

[0031] S3, cooling the mixture a obtained in step S2, adding the remaining humectant, Longjing green tea extract, pine plant extract solution obtained in step S1, and preservative, stirring and dissolving, homogenizing, adjusting pH with a neutralizing agent, filtering, and standing to obtain the cosmetic with cell repair effect.

[0032] Preferably, in the step S1, the sieving is performed through a 100-mesh sieve.

[0033] Preferably, in the step S1, the mass ratio of the pine plant extract to water is 1:10.

[0034] This step is to avoid the subsequent addition of pine plant extract clumps, which affects the skin care effect and formula stability.

[0035] Preferably, the pine plant extract is mixed separately.

[0036] Preferably, in the step S2, the humectant is glycerin, 1,3-propanediol, and sodium hyaluronate.

[0037] Preferably, in the step S3, the pH is adjusted to 5.5-6.5.

[0038] Preferably, in the step S3, the mixture a is first cooled to 45℃ before adding other components, in order to avoid high temperature damage to the activity of Longjing green tea extract and pine plant extract, thereby reducing the skin care effect.

[0039] Preferably, the stirring rate of S1 and S3 is 400 rpm.

[0040] The present application has the following beneficial effects:

[0041] The Longjing green tea extract, the Herba phrymarium extract and the Pinus massoniana extract are organically combined, the three are compounded, the antioxidation and the anti-inflammation are synergistically enhanced, the external damage is resisted, the cell damage is adjusted and repaired, the aging is delayed, sensitive discomfort is relieved, the natural ingredients are matched, the efficacy and mildness are considered, the multi-dimensional skin care demand is met, the skin care performance maximization is realized. BRIEF DESCRIPTION OF DRAWINGS

[0042] Figure 1 The hyaluronidase inhibition rate test results of different test samples. DETAILED DESCRIPTION

[0043] For the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples.

[0044] In the following examples and comparative examples, each raw material involved is a commercially available general material unless otherwise specified.

[0045] In the following examples and comparative examples:

[0046] The Herba phrymarium extract is purchased from Ningxia Xiangcao Biotechnology Co., Ltd., the Pinus massoniana extract is purchased from Shanghai Yuyu New Material Technology Co., Ltd., the Lycopodium clavatum extract is purchased from Shaanxi Bolin Biotechnology Co., Ltd., and the Pinus roxburghii extract is purchased from Wuhan Chengtian Fine Chemical Co., Ltd.

[0047] The Longjing green tea extract is a group variety, which is purchased from the Tea Research Institute of the Chinese Academy of Agricultural Sciences, and the preparation process is as follows:

[0048] S1, 10g of fresh and tender leaves of Xihu Longjing group variety is selected, rinsed with water to remove surface dust, dried at a low temperature of 40-45℃, and crushed into a powder of 20-40 mesh, and sealed for use;

[0049] S2, 1g of the powder is added with 15-20mL of deionized water, and the mixture is stirred and extracted at 60℃ for 2 hours, and the filtrate is combined and filtered with a 300 mesh filter cloth to remove residue;

[0050] S3, food-grade ethanol is slowly added to the filtrate to make the ethanol concentration reach 70% (volume fraction), and the mixture is placed at 4℃ for 12 hours, centrifuged at 3000r / min for 15 minutes, and the supernatant is taken, distilled at 40-50℃ under reduced pressure to remove ethanol, and the concentrated liquid is filtered through a 0.22μm microfiltration membrane, and the remaining amount is supplemented with butanediol to make the total weight parts reach 100 parts.

[0051] The preparation process of Xinyang Maojian tea extract is as follows: fresh green tea is selected, dried and crushed to 80 mesh. The green tea and deionized water are mixed in a mass ratio of green tea: deionized water = 1:10, and extracted at 75°C for 1.5 hours. After filtration, the filtrate is taken and left to stand for use as tea extract. Green tea, Yifutang tea industry authentic Mingqian special Xinyang Maojian, commercially available product.

[0052] Examples 1-5:

[0053] Examples 1-5 respectively provide a tea-pine composition with cell repair efficacy, components and weight ratio as shown in Table 1.

[0054] Table 1

[0055] Ingredients Example 1 Example 2 Example 3 Example 4 Example 5 Longjing green tea extract 5 2 4 5 3 Gansong root extract 3 1 2 1 3 Jinsong root extract 2 0.5 1 0.5 2

[0056] Comparative examples 1-5:

[0057] Comparative examples 1-5 respectively provide a tea-pine composition with cell repair efficacy, components and weight ratio as shown in Table 2.

[0058] Table 2

[0059] Ingredients Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Longjing green tea extract 10 / / 6 1.5 Gansong root extract / 10 / 0.5 7 Jinsong root extract / / 10 3 1.5

[0060] Application examples 1-5:

[0061] Application examples 1-5 respectively provide a serum with cell repair efficacy, and application examples 1-5 respectively include the tea-pine composition with cell repair efficacy described in examples 1-5, the specific formula table is shown in Table 3 (total weight parts are 100 parts).

[0062] Table 3 (unit: weight parts)

[0063]

[0064] The preparation method of the serum includes the following steps:

[0065] S1, the pine root extract and the pine root extract are respectively sieved on a 100 mesh sieve, and are respectively mixed with deionized water in a mass ratio of 1:10, heated to 40°C, and stirred until completely dissolved, to obtain a pine root extract solution and a pine root extract solution respectively;

[0066] S2, add the remaining deionized water to the stirring container, start stirring (400 rpm), and then add glycerol, 1,3-propanediol, and sodium hyaluronate in sequence, and stir until completely dissolved; then add carbomer and xanthan gum, and stir to form a uniform solution, heat to 75°C, and keep for 30 minutes to obtain a mixture a;

[0067] S3, the mixture a is cooled to below 45℃, and panthenol, tocopherol acetate, Longjing green tea extract, and the solution of Pinus masoniana extract and Pinus massoniana extract are added; after stirring uniformly, phenoxyethanol and ethylhexylglycerin are added, and stirring is performed until dissolution, and homogenization is performed at 2000 rpm; the pH of the mixture is adjusted to 5.5-6.5 with triethanolamine solution, filtration is performed, and standing is performed, to obtain the serum.

[0068] Application Comparative Examples 1-5:

[0069] Application Comparative Examples 1-5 each provide a serum having a cell repair effect, and each include the tea-pine composition having a cell repair effect described in Application Comparative Examples 1-5, and the specific formulation table is shown in Table 4 (total weight parts are 100 parts). The preparation method of the serum is the same as that of Example 1.

[0070] Table 4 (unit: weight parts)

[0071] Application Comparative Example 6:

[0072] This application comparative example provides a serum having a cell repair effect, which is different from Application Example 1 only in that the same amount of Xinyang Maojian tea extract is used to replace the Longjing green tea extract, and the content of the remaining components and the preparation method of the serum are the same as those of Application Example 1.

[0073] Application Comparative Example 7:

[0074] This application comparative example provides a serum having a cell repair effect, which is different from Application Example 1 only in that the same amount of Lycopodium clavatum extract is used to replace the Pinus masoniana extract, and the content of the remaining components and the preparation method of the serum are the same as those of Application Example 1.

[0075] Application Comparative Example 8:

[0076] This application comparative example provides a serum having a cell repair effect, which is different from Application Example 1 only in that the same amount of Pinus pinaster extract is used to replace the Pinus massoniana extract, and the content of the remaining components and the preparation method of the serum are the same as those of Application Example 1.

[0077] Application Comparative Example 9:

[0078] This application comparative example provides a serum having a cell repair effect, which is different from Application Example 1 only in that the pre-dissolution treatment (step S1) is not performed in the preparation process of this application comparative example (the raw materials of Pinus masoniana extract and Pinus massoniana extract are added in step S3), and the remaining steps are the same as those of Example 1.

[0079] Application Comparative Example 10:

[0080] The application comparative example provides a serum with cell repair efficacy, which is only different from the application example 1 in that the mixture a is not subjected to the cooling treatment in the preparation process, and the remaining components are directly added in step S3.

[0081] Effect example 1

[0082] The effect example takes the serums prepared from the application examples 1-5 and the application comparative examples 1-9 as test samples, and tests the anti-inflammatory effect of the serums.

[0083] The specific method is as follows:

[0084] 1. Reagents and materials

[0085] 1.1 Reagents: acetic acid-sodium acetate buffer (pH = 5.6), sodium hyaluronate solution, Ehrlich reagent, CaCl2 solution, NaOH solution, hyaluronidase solution, acetylacetone solution.

[0086] 1.2 Preparation of reagents:

[0087] 1.2.1 Preparation of acetic acid-sodium acetate buffer (pH = 5.6):

[0088] 1.155 mL of glacial acetic acid was diluted to 100 mL to prepare an acetic acid solution; 2.72 g of sodium acetate was weighed, dissolved in water, and then diluted to 100 mL to prepare a sodium acetate solution; 45.2 mL of the sodium acetate solution was added with 4.8 mL of the acetic acid solution, and then diluted to 100 mL after mixing to prepare the acetic acid-sodium acetate buffer (pH = 5.6).

[0089] 1.2.2 Sodium hyaluronate solution: 0.0050 g of sodium hyaluronate was weighed and dissolved in 10 mL of the acetic acid-sodium acetate buffer (pH = 5.6) to prepare a sodium hyaluronate solution with a mass concentration of 0.5 mg / mL.

[0090] 1.2.3 Ehrlich reagent: 0.8 g of p-dimethylaminobenzaldehyde was dissolved in 15 mL of concentrated hydrochloric acid and 15 mL of anhydrous ethanol, and stored in the dark.

[0091] 1.2.4 CaCl2 solution: 2.8 g of anhydrous calcium chloride was dissolved in 10 mL of deionized water and ultrasonicated until completely dissolved.

[0092] 1.2.5 NaOH solution: 0.16 g of NaOH was dissolved in 10 mL of deionized water and ultrasonicated until completely dissolved.

[0093] 1.2.6 Hyaluronidase solution: 0.0100 g of hyaluronidase was weighed and dissolved in 4 mL of acetic acid-sodium acetate buffer to prepare a hyaluronidase solution with a working concentration of 1250 U / mL, which was prepared before use.

[0094] 1.2.7 Acetylacetone solution: 3.5 mL of acetylacetone was measured and dissolved in 50 mL of sodium carbonate solution (1.0 mol / L) to prepare an acetylacetone solution, which was prepared before use.

[0095] 2. Instruments and equipment: analytical balance (accurate to 0.0001 g), 1 L volumetric flask, 50 mL graduated cylinder, 1000 μL pipette, constant temperature water bath, UV-Vis spectrophotometer.

[0096] 3. Determination steps

[0097] The test sample and reagent were sequentially added to the test tube according to Table 5, and after the reaction, the absorbance was detected at 547 nm, and the data was recorded.

[0098] Table 5: Composition of reaction solution (mL)

[0099]

[0100]

[0101] The absolute difference between the two independent determination results obtained under the repeatability conditions should not exceed 10% of the arithmetic mean. The hyaluronidase inhibition rate (%) was calculated according to formula (1), and the results are shown in Table 6.

[0102] Hyaluronidase inhibition rate (%) = [1 - (OD C - OD D ) / (OD A - OD B )] x 100%

[0103] Formula (1)

[0104] In the above formula, OD A is the absorbance value of the enzyme solution control group; OD B is the absorbance value of the enzyme solution control blank group; OD C is the absorbance value of the sample group; and OD D is the absorbance value of the sample blank control.

[0105] Table 6: Hyaluronidase inhibition rate effect of different test samples

[0106]

[0107] Table 6, Figure 1The results show that the antioxidant effect of the essence containing the tea-pine composition prepared by the application examples is better than that of the application examples.

[0108] Effect Example 2

[0109] In this effect example, the essence prepared by application example 1 and application comparative examples 1-10 is used as the test sample to perform skin tightening test, and the specific scheme is as follows:

[0110] Instrument: Laser confocal microscope LAICASP8, electronic balance Sartiorius BSA-224S.

[0111] Main reagent: DNA damage detection kit (γ-H2AX immunofluorescence method, Shanghai Biyun Tian Biotechnology Co., Ltd., C2035S).

[0112] Test principle: Active oxygen, ultraviolet radiation, etc. can cause DNA double-strand breakage, which is considered to be the most serious DNA damage. H2A histone family member X (H2AX) is one of the variants of chromatin histone H2A. When the cell DNA double-strand breaks, H2AX will rapidly undergo phosphorylation modification to form phosphorylated H2AX (γ-H2AX). The formation of γ-H2AX is a rapid response to DNA damage, therefore, γ-H2AX is an important DNA damage marker and is widely used in DNA damage and apoptosis research. The content level of γ-H2AX can effectively reflect the damage of DNA, and the stronger the fluorescence (green) intensity, the more serious the DNA damage. Ultraviolet radiation (UV): ultraviolet is a common DNA damage inducer. The cells are exposed to a certain dose of ultraviolet to simulate the damage of ultraviolet to cell DNA. Ultraviolet can make pyrimidine bases (such as thymine) in DNA molecules form pyrimidine dimers, causing DNA double helix structure to twist, and then trigger DNA double-strand breakage and other damages, so that the level of γ-H2AX increases.

[0113] Test sample: test group (application examples 1-5, application comparative examples 1-10), negative control group.

[0114] Test steps: Exponentially growing human skin fibroblast (HFB) cells were prepared at 5×10 4The holes are inoculated on 10mm laser confocal dishes, fixed in a constant temperature incubator at 37℃ with 5% CO2 for 24h, then the positive control group and the test group are irradiated with ultraviolet light for 10min, the test group is given 100μg / mL of test sample respectively, and incubated for 24h. The negative control group uses normal cell culture reagent without adding any factor that can cause DNA damage. Remove the culture medium containing the test sample, wash with PBS buffer solution for 2 times, add 1mL of immunostaining blocking solution. Remove the immunostaining blocking solution, add γ-H2AX rabbit monoclonal antibody, incubate at room temperature for 1h, remove the γ-H2AX rabbit monoclonal antibody, wash with PBS buffer solution for 2 times. Add anti-rabbit 488, incubate at room temperature for 1h, remove the anti-rabbit 488, wash with PBS buffer solution for 2 times. Give 100μL of DAPI, incubate for 30min. The treated cells are observed under a laser confocal microscope, the excitation wavelength is 488nm, and the emission wavelength is 525nm. The DNA damage repair efficacy test results are shown in Table 7.

[0115] Table 7

[0116]

[0117]

[0118] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application and are not intended to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A tea-pine composition with cell repair effects, characterized in that, It includes Longjing green tea extract and pine plant extract; the mass ratio of Longjing green tea extract to pine plant extract is Longjing green tea extract: pine plant extract = (2-5): (1.5-5).

2. The tea-pine composition with cell repair effects as described in claim 1, characterized in that, The mass ratio of Longjing green tea extract to pine plant extract is Longjing green tea extract: pine plant extract = (3-5): (1.5-5).

3. The tea-pine composition with cell repair effects as described in claim 1 or 2, characterized in that, The pine plant extracts include Nardostachys jatamansi root extract and Pinus massoniana root extract.

4. The tea-pine composition with cell repair effects as described in claim 3, characterized in that, The mass ratio of the Nardostachys jatamansi root extract and the Pineapple root extract is Nardostachys jatamansi root extract: Pineapple root extract = (1-3): (0.5-2).

5. The use of the tea-pine composition with cell repair effects as described in any one of claims 1-4 in the preparation of cosmetics.

6. A cosmetic product with cell repair function, characterized in that, The cosmetic product comprises a tea-pine composition with cell repair effects as described in any one of claims 1-4 and excipients; the excipients comprise at least one of a moisturizer, a neutralizer, a thickener, a preservative, and water.

7. The cosmetic product with cell repair function as described in claim 6, characterized in that, The cosmetic comprises the following components by weight percentage: 3.5-10% tea-pine combination with cell repair effects, 9.6-16.3% moisturizer, 0.1-0.4% neutralizer, 0.2-0.4% thickener, 0.9-1.2% preservative, and water as the balance.

8. The cosmetic product with cell repair function as described in claim 6, characterized in that, The humectant includes at least one of glycerin, 1,3-propanediol, panthenol, sodium hyaluronate, and tocopheryl acetate; and / or, the neutralizing agent includes triethanolamine; and / or, the thickener includes at least one of xanthan gum and carbomer; and / or, the preservative includes at least one of phenoxyethanol and ethylhexylglycerin.

9. The cosmetic product with cell repair function as described in claim 6, characterized in that, The dosage forms of the cosmetics include water, lotion, and cream.

10. A method for preparing a cosmetic product with cell repair function as described in any one of claims 6-9, characterized in that, Includes the following steps: S1. The pine plant extract is sieved and then mixed with some water to obtain a pine plant extract solution. S2. Add 8.1-13.3% humectant and thickener to the remaining water, stir well, heat and keep warm to obtain mixture a; S3. The mixture a obtained in step S2 is cooled down, and the remaining moisturizer, Longjing green tea extract, pine plant extract solution obtained in step S1 and preservative are added. The mixture is stirred, dissolved and homogenized, the pH is adjusted with a neutralizing agent, filtered, and allowed to stand to obtain the cosmetic with cell repair effect.

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