Bermuda grass slope protection base material and preparation and use method thereof

Abstract

The invention provides a bermuda grass slope protection base material and a preparation and use method thereof, and the preparation and use method comprises the following steps: S1, respectively preparing a bacillus amyloliquefaciens microbial inoculum, cellulose and humic acid, the humic acid takes bottom mud of rivers or lakes as a preparation raw material; s2, mixing the bacillus amyloliquefaciens microbial inoculum prepared in the step S1, cellulose, humic acid and polyacrylamide according to a certain proportion, wherein the mixing proportion is 2-3 parts by mass of the bacillus amyloliquefaciens microbial inoculum; 20-30 parts by mass of cellulose; 5-10 parts by mass of humic acid; 0.1 to 0.4 part by mass of polyacrylamide; and S3, the mixture obtained in the S2 is sieved, and the rapid field planting bermuda grass slope protection base material in the silty soil mud flat reclamation area is obtained. According to the method, the survival rate and disease resistance of the cynodon grass seeds can be improved under the drought condition, the water retention capacity of soil is improved, and meanwhile recycling of waste such as straw and bottom mud is achieved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of biotechnology, and particularly relates to a quick planting of Cynodon dactylon slope protection base in silty soil reclamation area and a preparation and use method thereof. BACKGROUND

[0002] The particle size composition of silty beach soil is mainly silty sand, and the contents of sand and clay are less. The silty sand content is mostly higher than 70%, and the clay content is generally not more than 20%. The silty beach soil has high salt content and poor physical structure, and is deficient in nutrient elements, which is a typical coastal saline soil. The average salt content of the soil profile is between 0.15 and 15.88 g / kg, and the salt return phenomenon is serious in summer. A large amount of white salt particles easily appear and gather on the surface of the soil, and conventional crops are difficult to survive and grow, which leads to crop failure. Research has proved that Cynodon dactylon, as a drought-tolerant, trample-tolerant and rapidly growing planted lawn, can be planted in silty soil beach reclamation and effectively realize soil fixation and slope protection. However, due to the poor water and fertilizer retention and supply capacity of silty beach soil, liquefaction and collapse often occur, which affects the planting effect of Cynodon dactylon.

[0003] Bacillus amyloliquefaciens, as a microorganism preparation with excellent characteristics, can secrete antifungal proteins, surfactin, iturin and other antibacterial substances to inhibit the growth of pathogenic bacteria. It has a wide range of application scenarios in promoting plant nutrient absorption, improving crop stress resistance and improving rhizosphere microecology. In addition, a large amount of crop straw such as rice and wheat is produced every year, and improper treatment of crop straw can cause adverse effects on the environment, society and economy. At the same time, due to factors such as nutrient salts and organic pollutants, the bottom mud of most rivers and lakes in China has the risk of causing eutrophication, algal bloom and black odor, and the existing bottom mud treatment methods cannot exclude the risk of secondary pollution.

[0004] Therefore, how to develop and produce suitable slope protection base to realize the improvement of silty soil beach soil, effectively promote the efficient planting of crops such as Cynodon dactylon, and at the same time alleviate the environmental pressure of straw burning and bottom mud treatment, is the main problem faced by technical personnel at present. SUMMARY

[0005] To solve the above problems, the present application provides a quick planting of Cynodon dactylon slope protection base in silty soil reclamation area and a preparation and use method thereof. The slope protection base made of crop straw, river bottom mud and microorganisms can improve the soil and fertilize, promote the planting and growth of plants, and significantly improve the slope protection effect of the easily collapsed reclamation area or embankment.

[0006] To achieve the above technical effects, the application provides a revetment base preparation method, which comprises the following steps: S1: preparing Bacillus amyloliquefaciens inoculum, cellulose and humic acid respectively, wherein the cellulose is prepared from crop straw, and the humic acid is prepared from river or lake sediment; S2: mixing the Bacillus amyloliquefaciens inoculum, cellulose, humic acid and polyacrylamide prepared in S1 according to a certain proportion, wherein the mixing proportion is as follows: 2-3 parts by mass of Bacillus amyloliquefaciens, 20-30 parts by mass of cellulose, 5-10 parts by mass of humic acid and 0.1-0.4 parts by mass of polyacrylamide; and S3: screening the mixture obtained in S2 to obtain a quick-planting cynodon revetment base in a silty soil reclamation area.

[0007] Optionally, the specific steps for preparing the Bacillus amyloliquefaciens inoculum in S1 comprise the following steps: S11a: taking Bacillus amyloliquefaciens out from -80℃, slowly melting to room temperature under natural conditions, inoculating into nutrient agar medium for culture, wherein the culture temperature is 37℃±1℃, and the culture time is 24±2 hours, to obtain activated Bacillus amyloliquefaciens single colony; S12a: inoculating the activated Bacillus amyloliquefaciens single colony obtained in S11a into a salt-improved PDA (Potato Dextrose Agar (Medium)) culture solution for stirring culture, wherein the culture temperature is 37℃±1℃, the stirring culture is performed by mechanical stirring, magnetic stirring or gas stirring, the stirring speed is 180 rpm, and the culture time is 8-12 hours, to obtain Bacillus amyloliquefaciens seed liquid; S13a: inoculating the Bacillus amyloliquefaciens seed liquid obtained in S12a into a fermentation culture solution for liquid fermentation, wherein the fermentation conditions are as follows: the initial pH value is 7.4-7.6, the fermentation temperature is 32℃±5℃, 100% air is introduced, the aeration amount is 1.2 V / (V·min), the stirring culture is performed by mechanical stirring, magnetic stirring or gas stirring, the mechanical stirring speed is 180 rpm, and the fermentation time is 36 hours, to obtain Bacillus amyloliquefaciens fermentation liquor; S14a: inoculating the Bacillus amyloliquefaciens fermentation liquor obtained in S13a into a solid culture medium for stacking fermentation, wherein the fermentation conditions are as follows: the fermentation temperature is 30-33℃, pure oxygen mixed gas is introduced, the oxygen to air ratio is 1:4-1:9, the dissolved oxygen aeration amount is 1-1.2 V / (V·min), the total stacking height is 3-5 cm, and the fermentation time is 3-5 days, to obtain fermented inoculum; S15a: drying the fermented inoculum obtained in S14a at 40-60℃ to a water content of 30-35%, to obtain Bacillus amyloliquefaciens solid inoculum, and then crushing the Bacillus amyloliquefaciens solid inoculum to obtain Bacillus amyloliquefaciens, wherein the solid inoculum contains >1.2×109 cfu / g of Bacillus amyloliquefaciens. -1 .

[0008] Optionally, the step of preparing the PDA culture solution with salt in S12a comprises: S121a: peel and cut a potato into small pieces, put them into water, boil until boiling, and then continue to boil for 30 minutes, cool to 25-30℃, and filter with gauze; S122a: add 20g sucrose and 1g inorganic salt to the filtrate obtained in S121a, wherein the inorganic salt is one or more of KCl, CaCl2, MgSO4, etc. Make up to 1000ml, sterilize at 115℃ for 30min, and prepare the PDA with salt.

[0009] Optionally, the step of preparing the fermentation culture solution in S13a comprises: S131a: peel and cut a potato into small pieces, put them into water, boil until boiling, and then continue to boil for 30 minutes, cool to 25-30℃, and filter with gauze; S132a: add 20g sucrose, 3g KH2PO4, 1.5g MgSO4, and 0.1-3.0mg / L thiamine to the filtrate obtained in S131a, make up to 1000ml, adjust the pH to 6.5-6.7, sterilize at 115℃ for 30min, and obtain the fermentation culture solution.

[0010] Optionally, the specific steps of preparing cellulose in step S1 comprise: S11b: crushing the solid mixture of wheat straw, straw and rice husk into small particles at a ratio of 5:3:2 or 3:5:2; S12b: using a planetary ball mill to grind the small particles of the mixture obtained in S11b at a self-rotation speed of 500-1000rpm and a revolution speed of 300-500rpm, and the grinding mode is continuous dry grinding or wet grinding to destroy the fiber structure; S13b: mixing the ground mixture obtained in S12b with an appropriate amount of water and transferring it to a high-pressure homogenizer to separate cellulose from plant cell walls, coarsely filtering through a 100-mesh screen, and finely filtering by vacuum filtration, plate and frame filter press or centrifugal separation, and drying the obtained filtrate in an oven at 50-60℃ to prepare cellulose.

[0011] Optionally, the specific steps for preparing humic acid in step S1 include: S11c: taking lake or river sediment, the lake sediment mainly consists of humus, and the content of humic acid is 30-60%, under the conditions of temperature 25-35 DEG C and relative humidity ≤60%, the sediment is dried in the dark for 7-15 days, and it is regularly turned over every 1-2 days; after being crushed, large-particle impurities are removed through a 100-mesh sieve to obtain crude sediment powder; S12c: after mixing the crude sediment powder obtained in S11c with an appropriate amount of water, the mixture is placed in a hydrothermal reaction kettle, and the reaction conditions are set as follows: reaction temperature 180-250 DEG C, reaction time 6-8 hours; after the reaction is completed, the reaction kettle is naturally cooled, the reaction liquid is separated by pressure filtration or centrifugation, and the concentrated product is dried under vacuum at 30-80 DEG C to prepare small-molecule humic acid mainly in the form of fulvic acid analogues.

[0012] Optionally, the Bacillus amyloliquefaciens is preserved in the Guangdong Microbial Culture Collection Center on November 11, 2021, the address of the preservation center is No. 59, Building 5, 100, Jiefang Road, Guangzhou, Guangdong Province, and the strain preservation number is GDMCC No: 62051.

[0013] Optionally, the strain of the Bacillus amyloliquefaciens is isolated from silty saline-alkali soil in Xinjiang Uygur Autonomous Region, and is obtained by isolating microorganisms in infertile saline-alkali soil through a salt-improved PDA culture medium; the strain of the Bacillus amyloliquefaciens has the following main biological characteristics: the colony is white or light yellow, round, smooth in surface, neat in edge, and viscous in texture; the physiological characteristics are as follows: gram-positive bacteria, bacilli, motile, capable of forming spores, and facultative anaerobic; the suitable growth temperature is 33 DEG C-35 DEG C, and the suitable pH is 7.0-7.2.

[0014] In the second aspect of the present application, a quick planting of kikuyu grass slope protection base in silty soil beach reclamation area is provided, which is prepared by the preparation method, and the slope protection base promotes the resistance of plants and increases the soil water and fertilizer retention performance through the antibacterial substances and metabolic products polyglutamic acid secreted by the growth-promoting microorganisms, wherein the microorganism is Bacillus amyloliquefaciens.

[0015] In the third aspect of the present application, a use method of the quick planting of kikuyu grass slope protection base in silty soil beach reclamation area is provided, which includes the following steps: s1: spreading the kikuyu grass seeds to the reclamation soil according to the amount of 8-12 kg / mu, and lightly pressing the kikuyu grass seeds into the soil; s2: uniformly spraying and covering the slope protection base to the surface of the grass seeds according to the mass ratio of 1:7-9 of the kikuyu grass seeds and the slope protection base; s3: using an atomizing pipe device for irrigation, the first irrigation needs to be poured thoroughly, and then each irrigation is performed once at 5:30-8:30 and 17:30-19:00 every day, so that the soil is kept wet for 5-7 days, and the kikuyu grass seeds germinate.

[0016] The present application has the following beneficial effects: (1) The Bacillus amyloliquefaciens strain is capable of adapting to drought, and by spraying it onto the ground and mixing it with the kikuyu grass seeds, the strain can continue to grow and secrete metabolites, effectively improving the survival rate of the grass seeds and the plant disease resistance.

[0017] (2) As a bio-based product, it has environmental friendly characteristics, and can realize high value-added conversion and utilization of waste such as straw and river sediment, reducing waste treatment cost and improving economic efficiency.

[0018] (3) Due to the naturalness and organic components of the raw materials, the prepared base material can be effectively converted into a seedling substrate or organic fertilizer after a period of use in slope protection, promoting the nutrient absorption of the planted plants, effectively improving the survival rate, and ensuring environmental protection.

[0019] Those skilled in the art will understand that the purposes and advantages that can be achieved by the present application are not limited to the above specific descriptions, and the above and other purposes that can be achieved by the present application will be more clearly understood according to the following detailed description. BRIEF DESCRIPTION OF DRAWINGS

[0020] The drawings described herein are used to provide further understanding of the present application, constitute a part of this application, and do not constitute a limitation on the present application; it should be noted that the pictures themselves are not within the protection scope of the present patent.

[0021] Figure 1 The figure is a schematic diagram of the preparation method of the slope protection base material in the embodiment of the present application; Figure 2 The figure is a schematic diagram of the use method of the slope protection base material in the embodiment of the present application; Figure 3 The figure is a schematic diagram of the specific application of the slope protection base material in the embodiment of the present application. DETAILED DESCRIPTION

[0022] In order to make the purposes, technical solutions and advantages of the present application more clear and explicit, the present application will be further described in detail below in combination with the embodiments and drawings, and the schematic embodiments of the present application and their descriptions are used to explain the present application, but not as a limitation on the present application. Here, it should be noted that in order to avoid obscuring the present application due to unnecessary details, only the structures and / or processing steps closely related to the scheme according to the present application are shown in the drawings, and other details not closely related to the present application are omitted.

[0023] It should be emphasized that the term "comprises / comprising" is used herein to indicate the presence of a feature, element, step or component, but does not exclude the presence or addition of one or more other features, elements, steps or components.

[0024] The embodiments of the present application will be described in detail below in combination with the drawings.

[0025] A first aspect of the embodiments of the present application is to provide a method for preparing a revetment base material, comprising the following steps: Figure 1 The method comprises the following steps: S1, preparing Bacillus amyloliquefaciens inoculum, cellulose and humic acid respectively, wherein the cellulose is prepared from crop straw, and the humic acid is prepared from river or lake sediment; S2, mixing the Bacillus amyloliquefaciens inoculum, cellulose, humic acid and polyacrylamide prepared in S1 according to a certain proportion, wherein the mixing proportion is as follows: 2-3 parts by mass of Bacillus amyloliquefaciens, 20-30 parts by mass of cellulose, 5-10 parts by mass of humic acid, and 0.1-0.4 parts by mass of polyacrylamide; and S3, sieving the mixture obtained in S2 to obtain a quick-planting cyperus rotundus revetment base material in a silty soil reclamation area.

[0026] Optionally, the specific steps for preparing the Bacillus amyloliquefaciens inoculum in S1 include the following steps: S11a, taking Bacillus amyloliquefaciens out from -80℃, slowly melting to room temperature under natural conditions, inoculating into nutrient agar medium for culture, wherein the culture temperature is 37℃±1℃, and the culture time is 24±2 hours, to obtain activated Bacillus amyloliquefaciens single colony; S12a, inoculating the activated Bacillus amyloliquefaciens single colony obtained in S11a into a salt-improved PDA (Potato Dextrose Agar (Medium)) culture solution for stirring culture, wherein the culture temperature is 37℃±1℃, the stirring culture is performed by mechanical stirring, magnetic stirring or gas stirring, the stirring speed is 180 rpm, and the culture time is 8-12 hours, to obtain Bacillus amyloliquefaciens seed liquid; S13a, inoculating the Bacillus amyloliquefaciens seed liquid obtained in S12a into a fermentation culture solution for liquid fermentation, wherein the initial pH value is 7.4-7.6, the fermentation temperature is 32℃±5℃, 100% air is introduced, the aeration amount is 1.2 V / (V·min), the stirring culture is performed by mechanical stirring, magnetic stirring or gas stirring, the mechanical stirring speed is 180 rpm, and the fermentation time is 36 hours, to obtain Bacillus amyloliquefaciens fermentation liquor; S14a, inoculating the Bacillus amyloliquefaciens fermentation liquor obtained in S13a into a solid culture medium for stacking fermentation, wherein the fermentation temperature is 30-33℃, pure oxygen mixed gas is introduced, the oxygen to air ratio is 1:4-1:9, the dissolved oxygen aeration amount is 1-1.2 V / (V·min), the total stacking height is 3-5 cm, and the fermentation time is 3-5 days, to obtain fermented inoculum; S15a, drying the fermented inoculum obtained in S14a at 40-60℃ to a water content of 30-35%, to obtain Bacillus amyloliquefaciens solid inoculum, and then crushing the Bacillus amyloliquefaciens solid inoculum to obtain Bacillus amyloliquefaciens, wherein the solid inoculum contains >1.2×109 cfu / g of Bacillus amyloliquefaciens. -1 .

[0027] Optionally, the step of preparing the PDA culture solution with salt in S12a comprises: S121a: 200 g of peeled and diced potatoes are put into water, boiled for 30 min, cooled to 25-30°C, and filtered with gauze; S122a: 20 g of sucrose and 1 g of inorganic salt are added to the filtrate obtained in S121a, wherein the inorganic salt is one or more of KCl, CaCl2, MgSO4, etc. The volume is adjusted to 1000 ml, and sterilized at 115°C for 30 min to prepare the PDA culture solution with salt.

[0028] Optionally, the step of preparing the fermentation culture solution in S13a comprises: S131a: peeled and diced potatoes are put into water, boiled for 30 min, cooled to 25-30°C, and filtered with gauze; S132a: 20 g of sucrose, 3 g of KH2PO4, 1.5 g of MgSO4, and 0.1-3.0 mg / L of thiamine are added to the filtrate obtained in S131a, the volume is adjusted to 1000 ml, the pH value is adjusted to 6.5-6.7, and sterilized at 115°C for 30 min to obtain the fermentation culture solution.

[0029] Optionally, the specific steps of preparing cellulose in step S1 comprise: S11b: straw, rice straw, and rice husk are mixed in a solid mixture at a ratio of 5:3:2 or 3:5:2, and the mixture is crushed into small particles; S12b: the small particles of the mixture obtained in S11b are ground with a planetary ball mill at a self-rotation speed of 500-1000 rpm and a revolution speed of 300-500 rpm, and the grinding mode is continuous dry grinding or wet grinding to destroy the fiber structure; S13b: the ground mixture obtained in S12b is mixed with an appropriate amount of water and transferred to a high-pressure homogenizer to separate cellulose from plant cell walls, and the obtained filtrate is pretreated by coarse filtration through a 100-mesh screen, and refined by vacuum filtration, plate and frame filter pressing, or centrifugal separation, and the obtained filtrate is dried in an oven at 50-60°C to obtain cellulose.

[0030] Optionally, the specific steps of preparing humic acid in step S1 include: S11c: taking lake or river sediment, the lake sediment mainly consists of humus, and the content of humic acid is 30-60%, under the conditions of temperature 25-35 DEG C and relative humidity less than or equal to 60%, the lake sediment is dried in the dark for 7-15 days, and the lake sediment is turned regularly every 1-2 days; after crushing, the lake sediment is sieved through a 100 mesh screen to remove large particle impurities, and a crude lake sediment powder is obtained; S12c: the crude lake sediment powder obtained in S11c is mixed with an appropriate amount of water, and then placed in a hydrothermal reaction kettle, and the reaction conditions are set as follows: reaction temperature 180-250 DEG C, reaction time 6-8 hours; after the reaction is completed, the reaction kettle is naturally cooled, the reaction liquid is separated by pressure filtration or centrifugation, and the reaction liquid is concentrated by vacuum drying at 30-80 DEG C to obtain small molecule humic acid mainly composed of fulvic acid analogues.

[0031] Optionally, the Bacillus amyloliquefaciens is preserved in the Guangdong Microbial Culture Collection Center on November 11, 2021, the address of the preservation center is No. 59, Building 5, 100, Martyrs' Road, Guangzhou, Guangdong Province, and the strain preservation number is GDMCC No: 62051.

[0032] Optionally, the strain of Bacillus amyloliquefaciens is separated from silty saline-alkali soil in Xinjiang Uygur Autonomous Region, and is obtained by separating microorganisms in poor saline-alkali soil through a salt-improved PDA culture medium; the main biological characteristics of the strain of Bacillus amyloliquefaciens are as follows: the colony is white or light yellow, round, smooth surface, neat edge, and viscous texture; the physiological characteristics are as follows: gram-positive bacteria, bacilli, motile, can form spores, and facultative anaerobic; the suitable growth temperature is 33 DEG C-35 DEG C, and the suitable pH is 7.0-7.2.

[0033] In a second aspect of the embodiments of the present application, a quick planting of Cynodon dactylon slope protection base in silty soil beach reclamation area is provided, which is prepared by the preparation method, and is characterized by that: the slope protection base promotes the resistance of plants and increases the soil water and fertilizer retention performance through the antibacterial substances and metabolic products polyglutamic acid secreted by the growth-promoting microorganisms, and the microorganisms are Bacillus amyloliquefaciens.

[0034] In a third aspect of the embodiments of the present application, as shown in Figure 2 The use method of the quick planting of Cynodon dactylon slope protection base in silty soil beach reclamation area includes the following steps: s1: the Cynodon dactylon seeds are sown in the reclamation soil at a quantity of 8-12 kg / mu, and the Cynodon dactylon seeds are lightly pressed into the soil; s2: the Cynodon dactylon seeds and the slope protection base are mixed at a mass ratio of 1:7-9, and the slope protection base is uniformly sprayed and covered on the surface of the seeds; s3: irrigation is performed by using an atomizing pipe device, the first irrigation needs to be poured thoroughly, and then each irrigation is performed once at 5:30-8:30 and 17:30-19:00 every day, the soil is kept wet for 5-7 days, and the Cynodon dactylon seeds germinate.

[0035] The application will be further described in detail below through specific examples, which are only descriptive and not restrictive, and cannot limit the protection scope of the application.

[0036] In this example, a mixture of wheat straw, rice straw and rice husk (mass ratio 3:5:2) was crushed into small particles, ground using a planetary ball mill, and after the fiber structure of the mixture was destroyed, an appropriate amount of water was added to transfer it to a high-pressure homogenizer to separate cellulose from the plant cell wall. After filtration and drying, the resulting product was cellulose, which was ready for use. A certain amount of lake or river sediment was air-dried, crushed, and sieved to remove large particle impurities. After mixing with an appropriate amount of water, it was placed in a hydrothermal reaction kettle, and the reaction conditions were as follows: 180-250℃, reaction time 6-8 hours. After the reaction was completed, the reaction kettle was naturally cooled, the reaction liquid was filtered, dried and concentrated, and the resulting product was humic acid, which was ready for use. The cellulose, humic acid, polyacrylamide and bacillus amyloliquefaciens inoculum were mixed and sieved (18 mesh, 1 mm aperture) to prepare a slope protection base material.

[0037] The slope protection base material was set up in 8 groups according to different mass proportions, and the specific proportions are shown in Table 1 (unit: mass parts): Table 1 Slope protection base material proportioning table under different treatments Control Bacillus amyloliquefaciens inoculant Cellulose Humic acid Polyacrylamide Treatment 1 2 20 5 0.1 Treatment 2 2 30 7 0.25 Treatment 3 2 25 6 0.25 Treatment 4 2 25 8 0.3 Treatment 5 2 28 8 0.35 Treatment 6 2.5 25 6 0.3 Treatment 7 3 20 5 0.25 Treatment 8 3 25 8 0.3 Treatment 9 3 30 7 0.2 Treatment 10 3 30 10 0.4 The reclamation area in Dongxin Farm (34°28'~34°37'N, 119°18'~119°30'E) in Lianyungang City, Jiangsu Province was selected as the test site. Dongxin Farm is located in the southern end of the warm temperate zone and has obvious marine climate characteristics. The average annual temperature is 13.7℃, the effective accumulated temperature above 10℃ is 3600-4700℃, the average annual rainfall is 930.2mm, the average annual evaporation is 1510.6mm, and the annual sunshine hours are 2200-2250 hours, with a frost-free period of 200-220 days. Dongxin Farm is located in the coastal saline soil area of northern Jiangsu and the warm temperate brown soil and brown soil belt. The surface layer is saline silt, and the salt content is generally 6-8g / kg.

[0038] On April 17, 2024 (average temperature 15-20℃), 10 kg of dog tooth grass seeds per mu of land were evenly sown on the beach soil in the reclamation area, and the seeds were pressed into the soil by light pressure; the slope protection base material was evenly sprayed and covered on the surface of the seeds at a ratio of dog tooth grass seeds: slope protection base material (mass ratio) = 1:8, and irrigation was carried out using atomizing pipe equipment after sowing. The first irrigation needed to be poured thoroughly, and then water was poured once in the morning and once in the evening every day to keep the soil moist until the seeds germinated (5-7 days). On May 30, 2024, the biomass of dog tooth grass, soil moisture content and salt content were measured. The aboveground biomass of dog tooth grass was measured by shearing method. Representative quadrats were selected, and the plants above ground were cut with scissors, and the aboveground parts were collected and weighed with an electronic balance. Then the samples were placed in an oven and dried to constant weight at 65-75℃, and then the dry weight was measured. The underground biomass was measured by soil drilling method. Soil samples were drilled in the lawn, the roots were collected and cleaned, and the fresh weight was measured with an electronic balance, and then dried to constant weight in an oven and weighed. The soil moisture content was measured by weight method, and the soil salinity was measured by conductivity method. Figure 3 Figure 1 shows a schematic diagram of the specific application of the slope protection base material of the present embodiment.

[0039] The aboveground biomass, underground biomass, soil moisture content and salt content of the reclamation beach soil under different treatments were calculated as shown in Table 2: Table 2 Detection of soil properties under different treatments Control Aboveground biomass kg / m2 Underground biomass kg / m2 Moisture content % Salinity content ‰ Control 0 0.02 0.04 15.13 7.85 Treatment 1 2.72 3.63 20.12 4.56 Treatment 2 2.12 3.52 17.60 6.43 Treatment 3 2.26 3.18 17.83 5.06 Treatment 4 2.94 3.70 19.05 5.01 Treatment 5 2.11 3.21 16.86 5.39 Treatment 6 2.64 3.90 18.32 6.00 Treatment 7 3.86 4.79 25.84 4.62 Treatment 8 3.01 4.00 22.43 5.01 Treatment 9 3.03 3.87 22.34 5.12 Treatment 10 2.90 3.54 18.88 5.75 Comparison 11 0.96 1.33 15.06 7.24 In the above table, control 0 is the original beach soil that has not been cultivated for 3 years in the reclamation area; comparison 11 refers to sowing 10 kg of dog tooth grass seeds per mu on the basis of control 0, and the rest of the plant protection management measures are consistent. The test found that treatment 7 had the best effect on customizing dog tooth grass for beach silty saline soil.

[0040] The Bacillus amyloliquefaciens strain used in the application is used as a microbial strain, and the metabolite polyglutamic acid is an anionic polypeptide polymer, and the iron carrier and potassium dissolving substances can promote the absorption of iron and potassium by plants, thereby enhancing the stress resistance of plants. In addition, the Bacillus amyloliquefaciens also has excellent water retention performance, and can be used as a soil conditioner to improve soil water retention and fertility, reduce water evaporation, and promote crop planting and soil improvement in silty beach reclamation areas. At the same time, the cellulose in the base material of the application is obtained from crop straw as raw material, and the humic acid is obtained from river or lake sediment as raw material, which can realize high value-added recovery and utilization of waste raw materials, meet the environmental protection expectation, and further improve the propagation effect of the Bacillus amyloliquefaciens strain. At the same time, the base material of the application can be used as a good seedling raising substrate or organic fertilizer after standing for a period of time (for example, 15-50 days), and the degradation of the corresponding organic matter also provides nutrients for the plants to be planted, such as dog tooth grass, which also achieves the purpose of environmental friendliness and resource recycling.

[0041] The above specific description further details the purpose, technical solution and beneficial effects of the application, and it should be understood that the above description is only a specific embodiment of the application and is not used to limit the protection scope of the application. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the application should be included in the protection scope of the application.

Claims

1. A method for preparing slope protection substrate, characterized in that, Includes the following steps: S1: Prepare Bacillus amyloliquefaciens inoculum, cellulose, and humic acid respectively, wherein: The cellulose is prepared from crop straw; The humic acid is prepared using river or lake sediment as raw material; S2: The Bacillus amyloliquefaciens inoculum, cellulose, humic acid, and polyacrylamide prepared in S1 are mixed in a certain proportion. The mixing proportion is as follows: 2-3 parts by weight of Bacillus amyloliquefaciens inoculum; 20-30 parts by weight of cellulose; 5-10 parts by weight of humic acid; Polyacrylamide 0.1–0.4 parts by weight; S3: The mixture obtained in S2 is sieved to obtain a rapid planting bermudagrass slope protection material for reclamation areas in silty soil tidal flats.

2. The preparation method according to claim 1, characterized in that, The specific steps for preparing the Bacillus amyloliquefaciens inoculum in step S1 include: S11a: Bacillus amyloliquefaciens was removed from -80℃ and slowly thawed to room temperature under natural conditions. It was then inoculated onto nutrient agar medium for culture at a temperature of 37℃±1℃ for 24±2 hours to obtain activated single colonies of Bacillus amyloliquefaciens. S12a: The activated Bacillus amyloliquefaciens single colony obtained from S11a was inoculated into salt-modified PDA culture medium and stirred for culture. The culture temperature was 37℃±1℃, and the culture was carried out by mechanical stirring, magnetic stirring or gas stirring. The stirring speed was 180 rpm, and the culture time was 8 to 12 hours to obtain Bacillus amyloliquefaciens seed culture. S13a: The Bacillus amyloliquefaciens seed culture obtained from S12a was inoculated into the fermentation culture medium for liquid fermentation. The fermentation conditions were as follows: initial pH value of 7.4-7.6, fermentation temperature of 32℃±5℃, 100% air was introduced at a flow rate of 1.2V / (V·min), and the culture was carried out by mechanical stirring, magnetic stirring or gas stirring. The mechanical stirring speed was 180rpm, and the fermentation time was 36 hours to obtain the Bacillus amyloliquefaciens fermentation broth. S14a: The Bacillus amyloliquefaciens fermentation broth obtained from S13a was inoculated into a solid culture medium and fermented. The fermentation conditions were as follows: fermentation temperature was 30-33℃, pure oxygen mixed gas was introduced, the oxygen to air ratio was 1:4-1:9, dissolved oxygen aeration rate was 1-1.2V / (V·min), the total height of the pile was 3-5cm, and the fermentation time was 3-5 days to obtain the fermented strain. S15a: The fermented inoculum obtained from S14a is dried at 40–60°C to a moisture content of 30–35% to obtain a solid inoculum of Bacillus amyloliquefaciens, which is then pulverized to obtain Bacillus amyloliquefaciens. The solid inoculum has a bacterial count >1.2 × 10⁹ cfug. -1 .

3. The preparation method according to claim 2, characterized in that, The preparation steps of the salt-modified PDA culture medium in S12a include: S121a: Peel and cut 200g of potatoes into small pieces and put them into water in every 1L of culture medium. Boil until boiling and continue boiling for 30 minutes. Cool to 25-30℃ and filter with gauze. S122a: Add 20g of sucrose and 1g of inorganic salt to the filtrate obtained in S121a, wherein the inorganic salt is one or more of KCl, CaCl2, MgSO4, etc. Adjust the volume to 1000ml and sterilize at 115℃ for 30min to prepare the salt-modified PDA.

4. The preparation method according to claim 2, characterized in that, The preparation steps of the fermentation culture medium in S13a include: S131a: In each 1L of culture medium, peel potatoes, cut them into small pieces, put them in water, boil them until boiling, continue boiling for 30 minutes, cool them to 25-30℃, and filter them with gauze. S132a: Add 20g sucrose, 3g KH2PO4, 1.5g MgSO4 and 0.1-3.0mg / L thiamine to the filtrate obtained from S131a, bring the volume to 1000ml, adjust the pH to 6.5-6.7, sterilize at 115℃ for 30min to obtain the fermentation culture.

5. The preparation method according to claim 1, characterized in that, The specific steps for preparing cellulose in step S1 include: S11b: crushing the solid mixture of wheat straw, rice straw and rice husk into small particles in a ratio of 5:3:2 or 3:5:2; S12b: grinding the small particles of mixture obtained in S11b using a planetary ball mill at a rotation speed of 500-1000 rpm and a revolution speed of 300-500 rpm, the grinding method being continuous dry grinding or wet grinding, to destroy its fiber structure; S13b: The small particles of the mixture obtained by grinding S12b are mixed with an appropriate amount of water and transferred to a high-pressure homogenizer to separate cellulose from the plant cell wall. The mixture is then pre-treated by coarse filtration through a 100-mesh sieve, followed by fine filtration using vacuum filtration, plate and frame filter press or centrifugation. The resulting filtrate is dried in an oven at 50-60℃ to prepare cellulose.

6. The preparation method according to claim 1, characterized in that, The specific steps for preparing humic acid in step S1 include: S11c: Take lake or river bottom sediment, which is mainly composed of humic substances and contains 30-60% humic acid. Under the conditions of 25-35℃ and relative humidity ≤60%, dry it in the dark for 7-15 days, turning it over regularly every 1-2 days. After crushing, pass it through a 100-mesh sieve to remove large particles of impurities, and obtain coarse bottom sediment powder. S12c: The crude sediment powder obtained from S11c is mixed with an appropriate amount of water and placed in a hydrothermal reactor. The reaction conditions are set as follows: reaction temperature 180-250℃, reaction time 6-8 hours. After the reaction is completed, the reactor is cooled naturally. The reaction liquid is separated by pressure filtration or centrifugation and then vacuum dried and concentrated at 30-80℃ to prepare small molecule humic acid mainly composed of fulvic acid analogs.

7. The preparation method according to claim 1, characterized in that: The Bacillus amyloliquefaciens strain was deposited on November 11, 2021, at the Guangdong Provincial Center for Microbial Culture Collection, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Guangdong Province, with accession number GDMCC No: 62051.

8. The preparation method according to claim 7, characterized in that: The strain of Bacillus amyloliquefaciens was isolated from silty saline-alkali soil in Xinjiang Uygur Autonomous Region and obtained by isolating microorganisms in barren saline-alkali land using salt-modified PDA medium. The main biological characteristics of the strain of *Bacillus amyloliquefaciens* are as follows: the colonies are white or light yellow, round, smooth, with neat edges, and viscous in texture; the physiological characteristics are as follows: Gram-positive, rod-shaped, motile, capable of forming spores, and facultatively anaerobic; the suitable growth temperature is 33℃-35℃, and the suitable pH is 7.0-7.

2.

9. A rapid-planting bermudagrass slope protection substrate for reclamation areas in silty soil tidal flats, prepared by the preparation method described in any one of claims 1-8, characterized in that: The slope protection substrate enhances plant resistance and increases soil water and fertilizer retention capacity by promoting the secretion of antibacterial substances and their metabolites, polyglutamic acid, by microorganisms. The microorganisms are Bacillus amyloliquefaciens.

10. A method for using the rapid planting of Bermuda grass slope protection material in silty soil reclamation areas as described in claim 8, characterized in that, Includes the following steps: s1: Sow Bermuda grass seeds at a rate of 8-12 catties / mu in the cultivated area soil and gently press the Bermuda grass seeds into the soil; s2: Mix bermudagrass seeds and slope protection base material at a mass ratio of 1:7 to 9, and evenly spray the slope protection base material to cover the surface of the grass seeds; S3: Use a misting device for irrigation. The first irrigation should be thorough. After that, water once a day from 5:30 to 8:30 and from 17:30 to 19:00, and keep the soil moist for 5 to 7 days until the bermudagrass seeds germinate.

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