Antibacterial kapok fiber-based transparent dressing and preparation method thereof

Abstract

The invention discloses an antibacterial kapok fiber-based transparent dressing and a preparation method thereof. The preparation method comprises the following steps: firstly, carrying out alkali treatment and mechanical pulverization on kapok fibers to prepare microfibrillated kapok fibers; carrying out bleaching treatment on the microfibrillated kapok fibers to remove lignin to obtain microfibrillated kapok fibers without lignin; mixing the lignin-removed microfibrillated kapok fibers with growth factors, and performing vacuum filtration to obtain a growth factor-loaded kapok fiber-based transparent dressing; and grafting the synthesized isocyanate L-borneol to the surface of the growth factor-loaded kapok fiber-based transparent dressing to finally obtain the antibacterial kapok fiber-based transparent dressing. The natural kapok fiber is used as a core raw material, and has the characteristics of greenness and degradability. The dressing has antibacterial property, transparency facilitating real-time monitoring of wound healing and a growth factor slow release function promoting healing.

Description

Technical Field

[0001] This invention relates to the field of medical dressings, specifically to an antibacterial kapok fiber-based transparent dressing and its preparation method. Background Technology

[0002] Wound healing is a crucial and complex physiological process in the human body. Poor healing can lead to chronic wounds, affecting an individual's daily life and work abilities, and in severe cases, even increasing the risk of amputation. During the wound healing process, doctors need to regularly observe the wound to assess its healing status. However, traditional dressings often need to be removed during observation, which can cause pain to the patient and disrupt the healing environment. In contrast, transparent dressings allow healthcare professionals to directly observe the wound, avoiding the discomfort caused to the patient and the interference with the healing environment during dressing removal.

[0003] Currently, most transparent dressings are made of synthetic polymers (such as polyurethane and polyethylene). These materials typically have limited functionality and lack features that further promote wound healing, such as antibacterial and drug release capabilities. Furthermore, these materials are difficult to degrade, and their environmental release after use poses a potential threat to ecosystems. Therefore, developing novel dressings is of great significance for improving wound treatment efficiency and reducing environmental impact. Summary of the Invention

[0004] To address the aforementioned problems, this invention provides an antibacterial kapok fiber-based transparent dressing and its preparation method. Kapok fiber, as a natural fiber, primarily comprises cellulose, hemicellulose, and lignin, which endow it with excellent biocompatibility and biodegradability. This dressing, using kapok fiber as its main material, effectively avoids the environmental pollution problems that may be caused by synthetic polymer materials, thus contributing to environmental sustainability. Furthermore, this dressing not only allows for real-time observation of the wound but also, by integrating antibacterial properties and growth factor release functions, further promotes wound healing.

[0005] This invention provides an antibacterial kapok fiber-based transparent dressing and its preparation method, using the following technical solution: In one aspect, the preparation method provided by the present invention includes the following steps: Step 1): Alkali treatment and mechanical crushing of kapok fibers to obtain microfibrillated kapok fibers; Step 2): The microfibrillated kapok fibers obtained in Step 1) are subjected to a bleaching process to remove lignin, thereby obtaining lignin-free microfibrillated kapok fibers. Step 3): Mix the lignin-free microfibrillated kapok fibers obtained in Step 2) with growth factors until homogeneous, and obtain a transparent kapok dressing loaded with growth factors by vacuum filtration. Step 4): Under nitrogen atmosphere, L-borneol and isophorone diisocyanate are mixed, dibutyltin dilaurate is added to catalyze the reaction, and L-borneol isocyanate is precipitated in petroleum ether to obtain a white solid. Step 5): Dissolve the isocyanate L-borneol obtained in Step 4) in N,N-dimethylformamide, then immerse the transparent kapok dressing obtained in Step 3) in the reaction, and finally dry it at room temperature to obtain the antibacterial kapok fiber-based transparent dressing.

[0006] Preferably, in step 1), the alkaline treatment is performed using an alkaline solution; the alkaline solution is a sodium hydroxide solution; the concentration of the alkaline solution is 1-25 wt%; the treatment time is 5-40 min; and the treatment temperature is 20-80℃.

[0007] Preferably, in step 1), the mechanical crushing is performed using a fiber crusher; the rotation speed of the fiber crusher is 2000-6000 rpm; and the crushing time is 1-10 min.

[0008] Preferably, the solution treated by the bleaching process in step 2) is prepared by dissolving sodium chlorite in a deionized aqueous solution to obtain a 1wt% sodium chlorite solution, and then adding acetic acid and stirring evenly, wherein the volume ratio of acetic acid to sodium chlorite solution is 20:1; the treatment temperature is 25-80℃; and the treatment time is 0.5-4h.

[0009] Preferably, the growth factor in step 3) is recombinant human epidermal growth factor or fibroblast growth factor-2; the concentration of the loaded growth factor is 1000-10000 IU / cm³. 2 The weight of the transparent kapok dressing is 0.0006-0.0024 g / cm³. 2 .

[0010] Preferably, in step 4), the molar ratio of levorotatory borneol, isophorone diisocyanate and dibutyltin dilaurate is 1:1.2-2:0.01-0.02; the reaction temperature is 40-80℃; and the reaction time is 8-16h.

[0011] Preferably, in step 5), the concentration of isocyanate L-borneol after dissolution is 2-10 wt%; and the immersion reaction time is 10-30 min.

[0012] In another aspect, the present invention provides an antibacterial kapok fiber-based transparent dressing prepared by the above-described preparation method.

[0013] Compared with the prior art, the beneficial effects of the present invention are: 1. The transparent dressing prepared by this invention enables real-time observation of the wound, avoiding secondary damage to the wound when conventional dressings are removed for observation.

[0014] 2. The transparent dressing prepared by this invention can effectively prevent bacterial adhesion through the use of isocyanate levonol, reducing the risk of infection. Compared with traditional broad-spectrum bactericidal methods, this targeted antibacterial strategy reduces unnecessary damage to the microbial community, thereby reducing the development of drug resistance. In addition, the good biocompatibility of the transparent dressing reduces inflammation and rejection reactions, which not only improves the safety of treatment, but also provides a more suitable environment for wound healing.

[0015] 3. The transparent dressing prepared by this invention can release growth factors that act on the wound, thereby accelerating wound healing.

[0016] 4. This invention uses natural kapok fiber as the core raw material, which has inherent green attributes and biodegradability. The use of natural kapok fiber not only conforms to the current environmental protection trend, but also effectively avoids the environmental burden caused by the difficulty of degrading synthetic materials because it can decompose naturally under the action of microorganisms. Attached Figure Description

[0017] Figure 1 This is a scanning electron microscope image of the surface of the antibacterial kapok fiber-based transparent dressing of Example 1 of the present invention; Figure 2 This is a comparative image showing the antibacterial properties of the antibacterial kapok fiber-based transparent dressing from Example 1 of the present invention. Figure 3 This is a scanning electron microscope image of the surface of the antibacterial kapok fiber-based transparent dressing of Embodiment 2 of the present invention; Figure 4 This is a comparative image showing the antibacterial properties of the antibacterial kapok fiber-based transparent dressing from Example 2 of the present invention. Figure 5 This is a scanning electron microscope image of the surface of the antibacterial kapok fiber-based transparent dressing of Example 3 of the present invention; Figure 6 This is a comparative image showing the antibacterial properties of the antibacterial kapok fiber-based transparent dressing from Example 3 of the present invention. Figure 7 This is a scanning electron microscope image of the surface of the antibacterial kapok fiber-based transparent dressing of Example 4 of the present invention; Figure 8 This is a comparative image showing the antibacterial properties of the antibacterial kapok fiber-based transparent dressing from Example 4 of the present invention. Detailed Implementation

[0018] The following are preferred embodiments of the present invention, which are only used to further illustrate the present invention and should not be considered as limiting the scope of protection of the present invention.

[0019] This invention discloses an antibacterial kapok fiber-based transparent dressing and its preparation method. The preparation method includes: firstly, alkali treatment and mechanical pulverization of kapok fibers to obtain microfibrillated kapok fibers; bleaching treatment to remove lignin from the microfibrillated kapok fibers to obtain lignin-free microfibrillated kapok fibers; mixing the lignin-free microfibrillated kapok fibers with growth factors, and obtaining a growth factor-loaded kapok fiber-based transparent dressing through vacuum filtration; and grafting synthetic isocyanate L-borneol onto the surface of the growth factor-loaded kapok fiber-based transparent dressing to finally obtain the antibacterial kapok fiber-based transparent dressing. This invention uses natural kapok fiber as the core raw material and has green and biodegradable properties. This dressing combines antibacterial properties, transparency for real-time monitoring of wound healing, and a sustained-release function of growth factors to promote healing.

[0020] Example 1 This invention provides a method for preparing an antibacterial kapok fiber-based transparent dressing, comprising the following steps: Step 1): Treat the kapok fiber with a 10wt% sodium hydroxide solution at 80℃ for 10 min, dry it at 60℃, and then put the alkali-treated kapok fiber into a fiber pulverizer and pulverize it at 2000 rpm for 5 min to obtain microfibrillated kapok fiber. Step 2): Dissolve sodium chlorite in deionized water to prepare a 1wt% sodium chlorite solution, then add acetic acid and stir until homogeneous, wherein the volume ratio of acetic acid to sodium chlorite solution is 20:1; then add the microfibrillated kapok fiber obtained in step 1) to the solution and disperse it evenly, and treat it at 80℃ for 1h to obtain white microfibrillated kapok fiber with lignin removed. Step 3): The white, lignin-free, microfibrillated kapok fibers obtained in Step 2) are mixed and stirred evenly with fibroblast growth factor-2. The mixture is then vacuum filtered to obtain a transparent kapok dressing loaded with fibroblast growth factor-2, wherein the concentration of fibroblast growth factor-2 is 1000 IU / cm³. 2 The weight of the transparent kapok dressing is 0.0006 g / cm³. 2 ; Step 4): Under nitrogen atmosphere, add L-borneol, isophorone diisocyanate and dibutyltin dilaurate catalyst in a molar ratio of 1:1.2:0.01, react at 50°C for 8 hours, and then precipitate in petroleum ether to obtain white L-borneol isocyanate solid. Step 5): Dissolve the isocyanate L-borneol obtained in Step 4) in N,N-dimethylformamide to prepare a 5wt% solution; then, immerse the transparent kapok dressing obtained in Step 3) in the solution and react for 10 minutes. Finally, dry at room temperature to obtain an antibacterial kapok fiber-based transparent dressing. The surface of the dressing is shown in the scanning electron microscope as follows: Figure 1 As shown.

[0021] like Figure 2 The antibacterial kapok fiber-based transparent dressing obtained in Example 1 has an antibacterial adhesion performance of 94.186%, a transparency of 88.971% in the visible light wavelength range, and a fibroblast growth factor-2 release rate of over 95% within 200 hours.

[0022] Example 2 This invention provides a method for preparing an antibacterial kapok fiber-based transparent dressing, comprising the following steps: Step 1): Treat the kapok fiber with a 10wt% sodium hydroxide solution at 80℃ for 10 min, dry it at 60℃, and then put the alkali-treated kapok fiber into a fiber pulverizer and pulverize it at 2000 rpm for 5 min to obtain microfibrillated kapok fiber. Step 2): Dissolve sodium chlorite in deionized water to prepare a 1 wt% sodium chlorite solution, then add acetic acid and stir until homogeneous, wherein the volume ratio of acetic acid to sodium chlorite solution is 20:1; then add the microfibrillated kapok fiber obtained in step 1) to the solution and disperse it evenly, and treat it at 80℃ for 2 h to obtain white microfibrillated kapok fiber with lignin removed. Step 3): The white, lignin-free, microfibrillated kapok fibers obtained in Step 2) are mixed and stirred evenly with recombinant human epidermal growth factor (rGF). The mixture is then vacuum-filtered to obtain a transparent kapok dressing loaded with rGF, wherein the concentration of rGF is 1000 IU / cm³. 2 The weight of the transparent kapok dressing is 0.0012 g / cm³. 2 ; Step 4): Under nitrogen atmosphere, add L-borneol, isophorone diisocyanate and dibutyltin dilaurate catalyst in a molar ratio of 1:1.2:0.01, react at 50°C for 8 hours, and then precipitate in petroleum ether to obtain white L-borneol isocyanate solid. Step 5): Dissolve the isocyanate L-borneol obtained in Step 4) in N,N-dimethylformamide to prepare a 5wt% solution; then, immerse the transparent kapok dressing obtained in Step 3) in the solution and react for 10 minutes. Finally, dry at room temperature to obtain an antibacterial kapok fiber-based transparent dressing. The surface of the dressing is shown in the scanning electron microscope as follows: Figure 3 As shown.

[0023] like Figure 4 The antibacterial kapok fiber-based transparent dressing obtained in Example 2 has an antibacterial adhesion performance of 92.105%, a transparency of 89.401% in the visible light wavelength range, and a recombinant human epidermal growth factor release rate of over 95% within 200 hours.

[0024] Example 3 This invention provides a method for preparing an antibacterial kapok fiber-based transparent dressing, comprising the following steps: Step 1): Treat the kapok fiber with a 10wt% sodium hydroxide solution at 80℃ for 10 min, dry it at 60℃, and then put the alkali-treated kapok fiber into a fiber pulverizer and pulverize it at 2000 rpm for 5 min to obtain microfibrillated kapok fiber. Step 2): Dissolve sodium chlorite in deionized water to prepare a 1wt% sodium chlorite solution, then add acetic acid and stir until homogeneous, wherein the volume ratio of acetic acid to sodium chlorite solution is 20:1; then add the microfibrillated kapok fiber obtained in step 1) to the solution and disperse it evenly, and treat it at 80℃ for 1h to obtain white microfibrillated kapok fiber with lignin removed. Step 3): The white, lignin-free, microfibrillated kapok fibers obtained in Step 2) are mixed and stirred evenly with recombinant human epidermal growth factor (rHGF). The mixture is then vacuum filtered to obtain a transparent kapok dressing loaded with rHGF, wherein the concentration of rHGF is 5000 IU / cm³. 2 The weight of the transparent kapok dressing is 0.0024 g / cm³. 2 ; Step 4): Under nitrogen atmosphere, add L-borneol, isophorone diisocyanate and dibutyltin dilaurate catalyst in a molar ratio of 1:1.2:0.01, react at 50°C for 10 h, and then precipitate in petroleum ether to obtain white L-borneol isocyanate solid. Step 5): Dissolve the isocyanate L-borneol obtained in Step 4) in N,N-dimethylformamide to prepare a 6wt% solution; then, immerse the transparent kapok dressing obtained in Step 3) in the solution and react for 20 minutes. Finally, dry at room temperature to obtain an antibacterial kapok fiber-based transparent dressing. The surface of the dressing is shown in the scanning electron microscope as follows: Figure 5 As shown.

[0025] like Figure 6 The antibacterial kapok fiber-based transparent dressing obtained in Example 3 has an antibacterial adhesion performance of 94.937%, a transparency of 89.384% in the visible light wavelength range, and a recombinant human epidermal growth factor release rate of over 95% within 200 hours.

[0026] Example 4 This invention provides a method for preparing an antibacterial kapok fiber-based transparent dressing, comprising the following steps: Step 1): Treat the kapok fiber with a 10wt% sodium hydroxide solution at 80℃ for 10 min, dry it at 60℃, and then put the alkali-treated kapok fiber into a fiber pulverizer and pulverize it at 2000 rpm for 5 min to obtain microfibrillated kapok fiber. Step 2): Dissolve sodium chlorite in deionized water to prepare a 1wt% sodium chlorite solution, then add acetic acid and stir until homogeneous, wherein the volume ratio of acetic acid to sodium chlorite solution is 20:1; then add the microfibrillated kapok fiber obtained in step 1) to the solution and disperse it evenly, and treat it at 80℃ for 1h to obtain white microfibrillated kapok fiber with lignin removed. Step 3): The white, lignin-free, microfibrillated kapok fibers obtained in Step 2) are mixed and stirred evenly with fibroblast growth factor-2. The mixture is then vacuum filtered to obtain a transparent kapok dressing loaded with fibroblast growth factor-2, wherein the concentration of fibroblast growth factor-2 is 10000 IU / cm³. 2 The weight of the transparent kapok dressing is 0.0024 g / cm³. 2 ; Step 4): Under nitrogen atmosphere, add L-borneol, isophorone diisocyanate and dibutyltin dilaurate catalyst in a molar ratio of 1:1.8:0.02, react at 50°C for 8 hours, and then precipitate in petroleum ether to obtain white L-borneol isocyanate solid. Step 5): Dissolve the isocyanate L-borneol obtained in Step 4) in N,N-dimethylformamide to prepare a 5wt% solution; then, immerse the transparent kapok dressing obtained in Step 3) in the solution and react for 20 minutes. Finally, dry at room temperature to obtain an antibacterial kapok fiber-based transparent dressing. The surface of the dressing is shown in the scanning electron microscope as follows: Figure 7 As shown.

[0027] like Figure 8 The antibacterial kapok fiber-based transparent dressing obtained in Example 4 has an antibacterial adhesion performance of 94.505%, a transparency of 89.746% in the visible light wavelength range, and a fibroblast growth factor-2 release rate of over 95% within 200 hours.

[0028] The above embodiments demonstrate that the kapok fiber-based transparent dressing prepared by the present invention has good antibacterial properties, good transparency, and a slow-release function of growth factors that promote healing.

[0029] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any form or substance. It should be noted that those skilled in the art can make various improvements and additions without departing from the present invention, and these improvements and additions should also be considered within the scope of protection of the present invention. Any modifications, alterations, and equivalent changes made by those skilled in the art based on the above-disclosed technical content without departing from the spirit and scope of the present invention are equivalent embodiments of the present invention. Furthermore, any modifications, alterations, and evolutions made to the above embodiments based on the essential technology of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A process for the preparation of an antibacterial kapok fiber based transparent dressing, characterized by, The method comprises the following steps: Step 1): alkali treatment and mechanical pulverization of kapok fibers to obtain microfibrillated kapok fibers; Step 2): bleaching process treatment of the microfibrillated kapok fibers obtained in step 1) to remove lignin and obtain microfibrillated kapok fibers with lignin removed; Step 3): mixing and stirring the microfibrillated kapok fibers with lignin removed obtained in step 2) with growth factors to obtain a kapok transparent dressing loaded with growth factors by vacuum filtration; Step 4): mixing levorotatory borneol with isophorone diisocyanate under a nitrogen atmosphere, adding dibutyltin dilaurate to catalyze the reaction, and precipitating in petroleum ether to obtain white solid isocyanate levorotatory borneol; Step 5): dissolving the isocyanate levorotatory borneol obtained in step 4) in N,N-dimethylformamide, then immersing the kapok transparent dressing obtained in step 3) in the reaction, and finally drying at room temperature to obtain an antibacterial kapok fiber-based transparent dressing.

2. The method of preparing an antibacterial kapok fiber based transparent dressing as claimed in claim 1, wherein, The alkali treatment in step 1) is treatment with an alkaline solution; The alkaline solution is a sodium hydroxide solution; the concentration of the alkaline solution is 1-25 wt%; the treatment time is 5-40 min; and the treatment temperature is 20-80℃.

3. The method of preparing the antibacterial kapok fiber based transparent dressing as claimed in claim 1, wherein, The mechanical pulverization in step 1) is pulverization using a fiber pulverizer; the rotational speed of the fiber pulverizer is 2000-6000 rpm; and the pulverization time is 1-10 min.

4. The method of preparing the antibacterial kapok fiber based transparent dressing as claimed in claim 1, wherein, The solution for bleaching process treatment in step 2) is prepared by dissolving sodium chlorite in a deionized water solution to obtain a 1 wt% sodium chlorite solution, then adding acetic acid and stirring uniformly, wherein the volume ratio of acetic acid to sodium chlorite solution is 20:1; the treatment temperature is 25-80℃; and the treatment time is 0.5-4 h.

5. The method of preparing the antibacterial kapok fiber based transparent dressing as claimed in claim 1, wherein, The growth factor in the step 3) is recombinant human epidermal growth factor or fibroblast growth factor-2; the concentration of the growth factor loaded is 1000-10000IU / cm 2 ; the grammage of the kapok transparent dressing is 0.0006-0.0024g / cm 2 .

6. The method of preparing the antibacterial kapok fiber based transparent dressing as claimed in claim 1, wherein, The molar ratio of levorotatory borneol, isophorone diisocyanate and dibutyltin dilaurate in step 4) is 1:1.2-2:0.01-0.02; the reaction temperature is 40-80℃; and the reaction time is 8-16 h.

7. The method of preparing the antibacterial kapok fiber based transparent dressing as claimed in claim 1, wherein, The concentration of the dissolved isocyanate levorotatory borneol in step 5) is 2-10 wt%; and the immersion reaction time is 10-30 min.

8. An antibacterial kapok fiber based transparent dressing characterized in that, The antibacterial kapok fiber-based transparent dressing prepared by the preparation method of any one of claims 1-7.

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