Moisturizing anti-wrinkle cream and preparation method thereof

Abstract

The invention provides moisturizing and anti-wrinkle cream and a preparation method thereof. The moisturizing and anti-wrinkle cream is prepared from the following raw materials in percentage by mass: 2%-10% of fibroin composite microsphere solution, 2%-5% of skin conditioner, 1%-2% of emollient, 8%-15% of humectant, 0.1%-1% of emulsifier, 1%-2% of emulsion stabilizer, 0.1%-0.2% of thickener and the balance of water. Wherein the fibroin composite microsphere is a spherical aggregate obtained by interpenetrating and entangling fibroin and poly-desoxyribonucleic acid in a cation environment and folding and compressing the fibroin and the poly-desoxyribonucleic acid; the composite microspheres can be percutaneously absorbed, and when the composite microspheres are applied to a formula of cream, the percutaneous permeation efficiency of the cream is greatly improved, and the moisturizing and anti-wrinkle effects of the cream are improved.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic preparation technology, specifically relating to a moisturizing and anti-wrinkle cream and its preparation method. Background Technology

[0002] Skin aging is a complex, multi-dimensional process that cannot be effectively addressed by single-function products. Internal aging and external environmental aggressors (such as UV rays and pollution) together lead to three core skin problems:

[0003] 1. Decreased water-locking function of the stratum corneum and loss of natural moisturizing factors lead to dryness and roughness (moisturizing needs).

[0004] 2. Damage to the sebum membrane and intercellular lipids (such as ceramides) weakens the barrier function, making it susceptible to irritation and triggering chronic micro-inflammation, thus accelerating aging (repair needs).

[0005] 3. The breakdown and loss of collagen and elastin in the dermis, and the repeated contraction of facial muscles lead to the solidification of dynamic wrinkles (anti-wrinkle needs).

[0006] These three factors are interconnected: dryness exacerbates barrier damage, the damaged barrier triggers inflammation and accelerates collagen degradation, and fragile skin cannot provide a good environment for the absorption of anti-wrinkle ingredients.

[0007] However, many existing products often have only one function or focus on one aspect, requiring consumers to use multiple products in combination. This not only makes the process cumbersome, but may also reduce the effectiveness or even increase the burden on the skin due to ingredient conflicts, sticky feeling, or excessive stimulation.

[0008] Therefore, against the backdrop of the current skincare market's consumption upgrade and the deep integration of scientific skincare concepts, developing a high-end cream product that integrates moisturizing and anti-wrinkle effects has become a key technological direction to respond to consumers' urgent need for efficient and simplified skincare processes. Summary of the Invention

[0009] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a cream with moisturizing and anti-wrinkle effects and its preparation method.

[0010] To achieve this objective, the present invention adopts the following technical solution:

[0011] This invention provides a moisturizing and anti-wrinkle cream, wherein the raw materials for preparing the moisturizing and anti-wrinkle cream include, by weight percentage:

[0012] Silk-core protein composite microsphere solution 2%~10%

[0013] Skin conditioning agent 2%~5%

[0014] Moisturizer 1%~2%

[0015] Moisturizer 8%~15%

[0016] Emulsifier 0.1%~1%

[0017] Emulsion stabilizer 1%~2%

[0018] Thickener 0.1%~0.2%

[0019] The remainder is water.

[0020] The cream provided by this invention is a cream that integrates moisturizing, anti-wrinkle and other effects. The ingredients in this cream are not a simple accumulation of single ingredients, but rather it is dedicated to solving the core problems of existing products such as fragmented efficacy, poor tolerance and low absorption rate through a systematic formula architecture.

[0021] The silk-core protein composite microspheres used in this invention have a small particle size, which allows them to pass through the cell membrane and enter the cell, achieving the effect of transdermal absorption. When used in conjunction with skin conditioning agents and moisturizers, the silk-core protein composite microspheres can help the skin conditioning agents and moisturizers penetrate the stratum corneum of the skin and enter the cell, so as to better exert the effects of moisturizing, anti-oxidation and repair of the active ingredients.

[0022] The silk-core protein composite microsphere solution comprises 2% to 10% by mass, for example, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%; the skin conditioning agent comprises 2% to 5% by mass, for example, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5%; the emollient comprises 1% to 2% by mass, for example, 1%, 1.2%, 1.5%, 1.6%, 1.8%, or 2%; and the moisturizer comprises 8% to 15% by mass, for example, 8%, 9%, 10 ... The percentages of emulsifiers are 1%, 10%, 11%, 12%, 13%, 14%, or 15%, etc.; the mass percentage of emulsifiers is 0.1% to 1%, for example, 0.1%, 0.2%, 0.5%, 0.6%, 0.8%, or 1%, etc.; the mass percentage of emulsion stabilizers is 1% to 2%, for example, 1%, 1.2%, 1.5%, 1.6%, 1.8%, or 2%, etc.; the mass percentage of thickeners is 0.1% to 0.2%, for example, 0.1%, 0.12%, 0.15%, 0.16%, 0.18%, or 2%, etc.

[0023] As a preferred technical solution of the present invention, the silk core protein composite microspheres are prepared by the following method: silk core protein and polydeoxyribonucleic acid (PDRN) are interpenetrated and folded and compressed under the action of polylysine to obtain the silk core protein composite microspheres.

[0024] Molecular docking simulations revealed a supramolecular interaction between PDRN and filamentin. This interaction was then used to induce supramolecular self-assembly between filamentin and PDRN through intermolecular forces. Furthermore, PDRN's foldable nature, characteristic of nucleic acids, was utilized to automatically compress and prepare complex aggregates with well-defined structures and specific functions. The resulting filamentin composite microspheres can penetrate cell membranes and enter cells, achieving transdermal absorption.

[0025] Preferably, the average particle size of the silk core protein composite microspheres is 50 nm to 500 nm, for example, it can be 50 nm, 100 nm, 150 nm, 200 nm, 250 nm, 300 nm, 320 nm, 330 nm, 340 nm, 350 nm, 360 nm, 380 nm, 400 nm, 420 nm, 450 nm, 480 nm or 500 nm, etc.

[0026] As a preferred technical solution of the present invention, in the raw materials for preparing the silk core protein composite microspheres, the mass ratio of silk core protein to polylysine is 1:(0.006~0.2), for example, it can be 1:0.006, 1:0.01, 1:0.02, 1:0.05, 1:0.1, 1:0.15 or 1:0.2, etc.

[0027] Preferably, the mass ratio of the silk core protein to polydeoxyribonucleic acid is 1:(1~10), for example, it can be 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9 or 1:10, etc.

[0028] Preferably, the mass ratio of polylysine to polydeoxyribonucleic acid is 1:(5~150), for example, it can be 1:5, 1:5, 1:10, 1:20, 1:50, 1:60, 1:80, 1:100, 1:120 or 1:150, etc.

[0029] Preferably, the mass percentage of fibroin in the fibroin composite microsphere solution is 1% to 5%, for example, it can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5% or 5%, etc.

[0030] Preferably, the mass percentage of polylysine in the silk core protein composite microsphere solution is 0.01% to 1%, and the polylysine is used to provide a cationic environment. Its mass percentage can be 0.01%, 0.02%, 0.05%, 0.1%, 0.2%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%, etc.

[0031] Preferably, the mass percentage of PDRN in the silk core protein composite microsphere solution is 1% to 5%, for example, it can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5% or 5%, etc.

[0032] Preferably, the polylysine comprises basic polylysine and / or modified polylysine.

[0033] Preferably, the polylysine is a mixture of basic polylysine and modified polylysine in a mass ratio of 1:(0.5~0.8), such as 1:0.5, 1:0.55, 1:0.6, 1:0.65, 1:0.7, 1:0.75 or 1:0.8.

[0034] The basic polylysine provides a cationic environment, which triggers the formation of complex microparticles. Replacing the basic polylysine with modified polylysine further improves transdermal absorption.

[0035] Preferably, the preparation of the modified polylysine includes the following steps:

[0036] Add the carboxyl group of phospholipid polyethylene glycol to water and stir evenly. Add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS), stir at 25~30℃ for 5~10 min, and dropwise add to a buffer solution containing basic polylysine (the buffer solution is a phosphate buffer solution with a pH of 7.4). Stir at 25~30℃ for 20~24 h, purify, and freeze-dry to obtain modified polylysine.

[0037] As a preferred embodiment of the present invention, the silk-core protein composite microsphere solution further contains a pH adjuster, and the pH of the silk-core protein composite microsphere solution is 5.5~6.5, for example, it can be 5.5, 5.6, 5.8, 6.0, 6.2, 6.3 or 6.5, etc.

[0038] Preferably, the pH adjuster includes any one or a combination of at least two of citric acid, lactic acid, malic acid, tartaric acid or glycolic acid, with citric acid being the most preferred.

[0039] Preferably, the silk-core protein composite microsphere solution further contains a thickener, and the content of the thickener in the silk-core protein composite microsphere solution is 0.5% to 1%, for example, it can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1%, etc.

[0040] Preferably, the thickener is hydroxyethyl cellulose.

[0041] In this invention, fibroin, PDRN, and polylysine can form the fibroin composite microspheres. Adding optional components such as thickeners and pH adjusters further facilitates storage and improves compatibility with other components. The amounts of thickener and pH adjuster used can be adjusted according to subsequent application requirements.

[0042] As a preferred embodiment of the present invention, the skin conditioning agent comprises any one or a combination of at least two of the following: vinyl polydimethylsiloxane / polymethylsiloxane sesquisiloxane cross-linked polymer, panthenol, tetrahydromethylpyrimidine carboxylic acid, allantoin, glyceryl glucoside, or silk amino acids.

[0043] Preferably, the skin conditioning agent is a combination of panthenol, allantoin, and silk amino acids, wherein the mass ratio of panthenol, allantoin, and silk amino acids is (1~30):(0.1~10):1; for example, it can be 1:0.1:1, 2:0.1:1, 5:0.1:1, 10:0.1:1, 15:0.1:1, 20:0.1:1, 25:0.1:1, 30:0.1:1, 1:1:1, 1:2:1, 1:5:1, 1:8:1, 1:10:1, etc.

[0044] Panthenol is a derivative of vitamin B5 (pantothenic acid). It is a colorless, transparent, highly moisturizing, and stable substance. Its effects on the skin are mainly reflected in moisturizing, repairing, anti-inflammatory, antioxidant, and anti-allergic properties. It can be absorbed by the skin or hair and converted into pantothenic acid, which participates in the metabolic process.

[0045] Allantoin is an amphoteric compound that can combine with various substances to form complex salts. It has photoprotective, antibacterial, antiseptic, analgesic, and antioxidant effects, and can help the skin retain moisture, keeping it hydrated and soft. Allantoin has functions such as protecting tissues, hydrophilicity, water absorption, and preventing moisture loss; allantoin promotes tissue growth, cell metabolism, and softens keratin.

[0046] Silk amino acids, with the INCI name SILKAMINOACIDS and CAS 96690-41-4, are a mixture of amino acids extracted from natural silk. These amino acids possess high physiological activity and biocompatibility. Silk amino acids are a highly active cosmetic and skincare ingredient, offering benefits such as moisturizing, nourishing the skin, inhibiting melanin formation, promoting skin tissue regeneration, and preventing cracking and chemical damage. They are widely used in cosmetics.

[0047] Preferably, the present invention also includes glycerol glucoside.

[0048] Glyceryl glucoside has moisturizing and antioxidant functions, and can also protect the stratum corneum from UV damage. After sun exposure, it helps cell regeneration, prevents collagen cross-linking caused by UV rays, inhibits cytokine formation, and prevents inflammation. It also has significant effects on restoring skin elasticity, reducing skin redness, repairing the skin barrier, and reducing wrinkles.

[0049] As a preferred embodiment of the present invention, the emollient includes any one or a combination of at least two of the following: triglyceride (ethylhexanoate), squalane, or meadowfoam seed oil.

[0050] Preferably, the emollient is a combination of squalane and meadowfoam seed oil; wherein the mass ratio of squalane to meadowfoam seed oil is 1:(0.1~1), for example, it can be 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.8, 1:0.9 or 1:1, etc.

[0051] Squalane is a lipid that is similar to human sebum. It has a strong affinity and can integrate with the human sebum membrane to form a natural barrier on the skin surface. It can also inhibit the peroxidation of skin lipids, effectively penetrate the skin, and promote the proliferation of skin basal cells. It has significant physiological effects on delaying skin aging and improving and eliminating age spots.

[0052] Meadowfoam (Limenthanes Alba) seed oil has a high content of long-chain fatty acids (over 98%) and a relatively low content of linoleic acid, thus exhibiting extremely strong antioxidant activity. The oxidative stability of meadowfoam seed oil is more than 10 times that of jojoba oil, making it one of the most stable plant oils. Studies have shown that meadowfoam seed oil has antioxidant and skin barrier repair effects, and it is widely used in cosmetics, especially in essential oil products.

[0053] Preferably, the moisturizer includes any one or a combination of at least two of erythritol, 1,3-propanediol, glycerin, tranexamic acid, trehalose, or 1,2-hexanediol.

[0054] Trehalose has strong moisturizing properties and various physiological benefits such as sun protection and UV protection.

[0055] As a preferred embodiment of the present invention, the emulsifier includes polyglycerol-3-methylglucose distearate.

[0056] Preferably, the emulsifier stabilizer comprises sodium acrylate / sodium acryloyldimethyl taurate copolymer.

[0057] Preferably, the thickener comprises an acrylate / C10-30 alkanol acrylate crosspolymer.

[0058] As a preferred embodiment of the present invention, the repair cream further includes a pH adjuster.

[0059] Preferably, the pH value of the moisturizing and anti-wrinkle cream is 4.0 to 8.5, for example, it can be 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0 or 8.5.

[0060] Preferably, the pH adjuster is aminomethylpropanol.

[0061] Secondly, a method for preparing a moisturizing and anti-wrinkle cream as described in the first aspect, the method comprising the following steps:

[0062] The formula is prepared by mixing the silk protein composite microsphere solution, skin conditioning agent, emollient, moisturizer, emulsifier, emulsion stabilizer, thickener and water, stirring, and after sampling and testing to ensure it passes the test, it is then filled to obtain the cream.

[0063] In this invention, the cream preparation method involves mixing the required raw materials according to the dissolution temperature to ensure that the final cream composition is stable, without layering or precipitation.

[0064] As a preferred technical solution of the present invention, the preparation steps are as follows:

[0065] Phase A consists of panthenol, allantoin, erythritol, 1,3-propanediol, glycerol, trehalose, and acrylate / C10-30 alkanol acrylate cross-linked polymers; Phase B consists of glycerol triethylhexanoate, squalane, meadowfoam seed oil, and polyglycerol-3-methylglucopyranoside distearate; Phase C consists of vinyl polydimethylsiloxane / polymethylsiloxane sesquisiloxane cross-linked polymers and sodium acrylate / sodium acryloyldimethyltaurate copolymers; Phase D consists of aminomethylpropanol; and Phase E consists of silk protein composite microsphere solution, tetrahydromethylpyrimidine carboxylic acid, glyceryl glucoside, silk amino acids, tranexamic acid, and 1,2-hexanediol.

[0066] (1) Add phase A to the emulsifying pot, homogenize, stir and heat to dissolve; add phase B to the oil pot, stir and heat to dissolve;

[0067] (2) Maintain a vacuum state, transfer phase B in the oil pan to the emulsification pan, add phase C, emulsify and homogenize, keep warm and defoam;

[0068] (3) Cool down, add phase D and stir evenly, and continue to cool down;

[0069] (4) Cool down and add phase E and stir until homogeneous;

[0070] (5) Cool down to 30~38℃, stop stirring, and obtain the cream.

[0071] As a preferred technical solution of the present invention, the preparation method of the silk core protein composite microsphere solution is as follows: polydeoxyribonucleic acid is added to water and stirred evenly to obtain an aqueous solution of polydeoxyribonucleic acid;

[0072] Dilute the silk protein solution, mix it with an aqueous solution of polydeoxyribonucleic acid, add an aqueous solution of polylysine to obtain a composite solution system, and stir to mix.

[0073] Then add an optional pH adjuster and thickener, and stir to obtain the silk core protein composite microsphere solution.

[0074] As a preferred technical solution of the present invention, the dissolution temperature of phase A is 80℃~90℃, for example, it can be 80℃, 81℃, 82℃, 83℃, 84℃, 85℃, 86℃, 87℃, 88℃, 89℃ or 90℃, etc.

[0075] Preferably, the dissolution temperature of phase B is 80℃~90℃, for example, it can be 80℃, 81℃, 82℃, 83℃, 84℃, 85℃, 86℃, 87℃, 88℃, 89℃ or 90℃, etc.

[0076] Preferably, the temperature at which phase D is added is 70°C to 80°C, for example, it can be 70°C, 71°C, 72°C, 73°C, 74°C, 75°C, 76°C, 77°C, 78°C, 79°C or 80°C.

[0077] Preferably, the temperature at which the E phase is added is 40°C to 50°C, for example, 40°C, 42°C, 44°C, 45°C, 48°C, or 50°C.

[0078] The numerical range described in this invention includes not only the point values ​​listed above, but also any point values ​​within the numerical ranges not listed above. Due to space limitations and for the sake of brevity, this invention will not exhaustively list all the specific point values ​​included in the range.

[0079] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0080] The cream provided by this invention includes silk protein composite microspheres, panthenol, allantoin, silk amino acids, squalane, and meadowfoam seed oil, etc. The effective ingredients are reasonably combined, which has good use effect, good skin feel, and obvious moisturizing and anti-wrinkle effect.

[0081] The silk-core protein composite microspheres are characterized by small particle size and high transdermal absorption rate. When used in combination with skin conditioning agents such as panthenol, allantoin, and silk amino acids, and emollients such as squalane and meadowfoam seed oil, they can maximize the function of various active ingredients and achieve multiple effects such as moisturizing, repairing, and anti-wrinkle. Experiments have shown that after 4 weeks of use, the average wrinkle depth of volunteers changed by more than 10%, with a maximum of 15.68%; the water content changed by more than 18%, with a maximum of 22.71%. Detailed Implementation

[0082] The technical solution of the present invention will be further illustrated below through specific embodiments. However, the following examples are merely simplified examples of the present invention and do not represent or limit the scope of protection of the present invention. The scope of protection of the present invention shall be determined by the claims.

[0083] In the following embodiments, the experimental materials and consumables were all purchased from conventional reagent manufacturers in the art; unless otherwise specified, the experimental methods and techniques used are conventional methods and techniques in the art.

[0084] Among them, silk heart protein can be purchased from Suzhou Suhao Biomaterials Co., Ltd.; PDRN can be purchased from Ruijiming Biotechnology Co., Ltd. or Shanghai Huiwen Biotechnology Co., Ltd.; polylysine can be purchased from Shandong Freda Biotechnology Co., Ltd. or Shanghai Maclean Biochemical Technology Co., Ltd.; vinyl polydimethylsiloxane / polymethylsiloxane sesquisiloxane cross-linked polymer can be purchased from Qingyuan Ruixin Biotechnology Co., Ltd.; allantoin can be purchased from Shandong Ailitong New Materials Co., Ltd.; glycerol glucoside can be purchased from Guangdong Xuanjia Medical Health Technology Co., Ltd.; silk amino acids can be purchased from Beijing Aosimei Biotechnology Co., Ltd., under the product name Sishuyuan; squalane can be purchased from Yichun Dahai Turtle Life Science Co., Ltd.; Limenthanhes Alba seed oil can be purchased from Elementis Specialties, Inc.; glycerol can be purchased from Procter & Gamble International Operations SA Singapore Branch; tranexamic acid can be purchased from Shaanxi Yueda Tianrun Biotechnology Co., Ltd.; 1,2-hexanediol can be purchased from Microcare Emollient HXD(P). Tor Chemicals; polyglycerol-3-methylglucose distearate is available from Evonik Operations GmbH; sodium acrylate / sodium acryloyldimethyl taurate copolymer is available from Guangzhou Baifurun Chemical Co., Ltd.; acrylate / C10-30 alkanol acrylate crosspolymer is available from Lubrizol Advanced Materials, Inc.

[0085] All other materials not mentioned can be purchased from conventional manufacturers in this field.

[0086] The silk-core protein composite microsphere solution described in this invention can be prepared according to the following method:

[0087] (1) Dissolve 1 g of PDRN (DNA content ≥70%, purchased from Ruijiming Biotechnology Co., Ltd.) in 50 g of water and stir until homogeneous to obtain PDRN solution; dissolve 1 g of silk protein (purchased from Suzhou Suhao Biomaterials Co., Ltd.) in 25 g of water and stir until homogeneous; then dissolve 0.05 g of polylysine (purchased from Shandong Freda Biotechnology Co., Ltd.) in 25 g of water and stir.

[0088] (2) Mix the silk core protein solution prepared in step (1) with the PDRN solution and stir for 60 min at a stirring speed of 600 rpm. Then, add the polylysine solution and stir at 600 rpm for 30 min.

[0089] (3) Add 0.5% by mass of hydroxyethyl cellulose (purchased from Beijing Kangtailong Technology Development Co., Ltd., molecular weight and viscosity 3400~5000 mPa.s), and then use citric acid to adjust the pH of the solution to about 6 to obtain an aqueous solution containing the silk core protein composite microspheres, wherein the particle size of the silk core protein composite microspheres is <500 nm.

[0090] Example 1

[0091] This embodiment provides a moisturizing and anti-wrinkle cream, the raw materials of which are shown in Table 1, including:

[0092] Table 1

[0093] Serial Number name mass percentage effect 1 Silk core protein composite microsphere solution 5 Skin protectant 2 Vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane crosslinked polymer 2 Skin conditioning agent 3 Panthenol 0.3 Skin conditioning agent 4 Tetrahydromethylpyrimidine carboxylic acid 0.1 Skin conditioning agent 5 Allantoin 0.1 Skin conditioning agent 6 Glyceryl glucoside 0.1 Skin conditioning agent 7 Silk amino acids 0.01 Skin conditioning agent 8 Glyceryl triethylhexanoate 1 Moisturizer 9 squalane 0.1 Moisturizer 10 Meadowfoam (Limenthanes Alba) seed oil 0.1 Moisturizer 11 Erythritol 3 Moisturizer 12 1,3-Propanediol 3 Moisturizer 13 glycerin 2 Moisturizer 14 tranexamic acid 0.5 Moisturizer 15 Trehalose 0.5 Moisturizer 16 1,2-Hexanediol 0.5 Moisturizer 17 Polyglycerol-3-methylglucose distearate 0.4 emulsifier 18 Sodium acrylate / sodium acryloyldimethyl taurate copolymer 1.5 Emulsion stabilizers 19 Acrylic (ester) crosspolymers / C10-30 alkanol acrylate crosspolymers 0.2 Thickener 20 aminomethylpropanol 0.13 pH adjuster 21 water Replenish to 100%

[0094] The preparation method is as follows:

[0095] Phase A consists of panthenol, allantoin, erythritol, 1,3-propanediol, glycerol, trehalose, and acrylate / C10-30 alkanol acrylate cross-linked polymers; Phase B consists of glycerol triethylhexanoate, squalane, meadowfoam seed oil, and polyglycerol-3-methylglucopyranoside distearate; Phase C consists of vinyl polydimethylsiloxane / polymethylsiloxane sesquisiloxane cross-linked polymers and sodium acrylate / sodium acryloyldimethyltaurate copolymers; Phase D consists of aminomethylpropanol; and Phase E consists of silk protein composite microsphere solution, tetrahydromethylpyrimidine carboxylic acid, glyceryl glucoside, silk amino acids, tranexamic acid, and 1,2-hexanediol.

[0096] (1) Add phase A to the emulsifying pot, turn on the homogenizer, homogenize for 5-8 minutes, stir and heat to 85°C, keep warm for 10 minutes to ensure complete dissolution; add phase B to the oil pot, stir and heat to 80°C to dissolve completely.

[0097] (2) Keep the emulsifying pot under vacuum (-0.05 MPa), adjust the homogenization to medium-high speed, draw phase B into the emulsifying pot, add phase C at the same time, emulsify and homogenize for 5 minutes, keep warm and defoam for 20 minutes to ensure no bubbles, and start cooling.

[0098] (3) Cool down to 75°C, add phase D and stir well, then continue cooling;

[0099] (4) Cool down to 45°C, add phase E and stir until homogeneous; among them, tetrahydromethylpyrimidine carboxylic acid, silk amino acids and tranexamic acid should be completely dissolved in an appropriate amount of water in advance;

[0100] (5) Cool down to 38°C, stop stirring, and obtain the cream.

[0101] Example 2

[0102] This embodiment provides a moisturizing and anti-wrinkle cream, which differs from Embodiment 1 in that:

[0103] The composition includes: 8% silk protein composite microsphere solution, 1.5% vinyl polydimethylsiloxane / polymethylsiloxane sesquisiloxane cross-linked polymer, 0.2% panthenol, 0.2% tetrahydromethylpyrimidine carboxylic acid, 0.3% allantoin, 0.3% glycerol glucoside, and 0.1% silk amino acids.

[0104] The remaining components and preparation methods are consistent with those in Example 1.

[0105] Example 3

[0106] This embodiment provides a moisturizing and anti-wrinkle cream, which differs from Embodiment 1 in that:

[0107] The composition includes 2% silk-core protein composite microsphere solution, 1.5% triglyceride (ethylhexanoate), 0.2% squalane, and 0.05% meadowfoam (Limenthanes Alba) seed oil.

[0108] The remaining components and preparation methods are consistent with those in Example 1.

[0109] Example 4

[0110] This embodiment provides a moisturizing and anti-wrinkle cream;

[0111] The difference from Example 1 is that the skin conditioning agent does not contain allantoin, and the missing part is made up by a vinyl polydimethylsiloxane / polymethylsiloxane sesquioxane crosslinked polymer.

[0112] Example 5

[0113] This embodiment provides a moisturizing and anti-wrinkle cream;

[0114] The difference from Example 1 is that the skin conditioning agent does not contain glyceryl glucoside, and the missing part is made up by vinyl polydimethylsiloxane / polymethylsiloxane sesquioxane cross-linked polymer.

[0115] Example 6

[0116] This embodiment provides a moisturizing and anti-wrinkle cream;

[0117] The difference from Example 1 is that the skin conditioning agent does not contain squalane, and the missing part is made up by meadowfoam seed oil.

[0118] Example 7

[0119] This embodiment provides a moisturizing and anti-wrinkle cream;

[0120] The difference from Example 1 is that the skin conditioning agent does not contain meadowfoam seed oil, and the missing part is made up by squalane.

[0121] Example 8

[0122] This embodiment provides a moisturizing and anti-wrinkle cream;

[0123] The difference from Example 1 is that the silk amino acids in the skin conditioning agent are replaced with amino acid moisturizers.

[0124] Comparative Example 1

[0125] This comparative example provides a cream that differs from Example 1 in that it does not contain a silk core protein composite microsphere solution, and the missing portion is made up by a vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane crosslinked polymer.

[0126] Comparative Example 2

[0127] This comparative example provides a cream that differs from Example 1 in that it does not contain emollients, and the missing portion is made up by a silk core protein composite microsphere solution.

[0128] Comparative Example 3

[0129] This comparative example provides a cream that differs from Example 1 in that it does not contain an emulsifying stabilizer, and the missing portion is made up by a silk core protein composite microsphere solution.

[0130] However, the cream prepared in this comparative example had poor stability.

[0131] Comparative Example 4

[0132] This comparative example provides a cream that differs from Example 1 in that the content of the silk core protein composite microsphere solution is 1%, and the missing part is made up by a vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane crosslinked polymer.

[0133] Comparative Example 5

[0134] This comparative example provides a cream that differs from Example 1 in that the content of the vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane crosslinked polymer is 1%, and the missing part is made up by the silk core protein composite microsphere solution.

[0135] Comparative Example 6

[0136] This comparative example provides a cream that differs from Example 1 in that the content of the silk core protein composite microsphere solution is replaced with an equal amount of silk core protein and PDRN; the rest remains the same as in Example 1.

[0137] Test Example 1

[0138] Human skin patch experiment

[0139] Participants: 30 in total, 0 males and 30 females, aged 18-40 years, meeting the criteria for voluntary inclusion.

[0140] Patch method: Select a qualified patch tester and use a closed patch test method. Take about 0.1 g of Examples 1-8 and Comparative Examples 1-6 and apply it to the patch tester. Apply it to the back of the subject with adhesive tape. Remove the test substance after 24 h. Observe the skin reaction at 0.5 h, 24 h and 48 h after removal. Record the results according to the skin reaction grading standard in the "Cosmetic Hygiene Standard".

[0141] Human skin patch test results showed that none of the 30 subjects developed symptoms such as erythema, edematous erythema, significant redness or swelling, or papules; indicating that the creams prepared in Examples 1-8 and Comparative Examples 1-6 are safe to use.

[0142] Test Example 2

[0143] Skin texture and skin hydration test

[0144] (1) Test subjects: For each example sample, volunteers (28-50 years old) were selected. Subjects were randomly grouped and the number of subjects per sample was ≥30. Two samples could be tested on the left and right sides of a single person.

[0145] (2) Method of use: After cleansing the face, apply the creams of Examples 1-8 and Comparative Examples 1-6 to the face. Do not replace or combine with other similar products during the test.

[0146] (3) Testing instruments: EVA FACE rapid optical imaging system for skin to analyze facial wrinkles; MPA580 skin tester to test skin hydration and other indicators.

[0147] (4) Testing method: The volunteers were tested once the day before using the sample and once after 4 weeks of use; the volunteers' skin was kept clean before the test and they sat quietly indoors for 30 minutes before the test.

[0148] The texture of the skin surface was analyzed and tested using the EVA FACE rapid optical imaging system. The texture of the skin is closely related to the plumpness of cells and the density between cells. The plumper the cells, the higher the water content; the higher the density between cells, the smoother the skin texture.

[0149] Skin hydration was tested using the MPA580 skin analyzer, and the results are shown in Table 2.

[0150] Table 2

[0151] sample Rate of change of average wrinkle depth Rate of change in water content Example 1 15.68% 22.71% Example 2 13.79% 20.65% Example 3 10.02% 18.27% Example 4 12.21% 20.73% Example 5 10.54% 18.54% Example 6 12.44% 15.49% Example 7 10.95% 16.91% Example 8 11.17% 17.19% Comparative Example 1 5.43% 8.20% Comparative Example 2 8.01% 13.21% Comparative Example 3 10.33% 19.25% Comparative Example 4 8.29% 14.06% Comparative Example 5 9.97% 15.12% Comparative Example 6 5.74% 8.74%

[0152] Based on the data analysis in the table above, it can be seen that the wrinkle improvement and water content change rate of the moisturizing and anti-wrinkle creams prepared in Examples 1-8 are better than those prepared in the comparative examples; among them, the repair effect of comparative example 3 is close to that of the examples, but its stability is poor because it does not contain emulsifying stabilizers.

[0153] Meanwhile, comparing Example 1 with Comparative Examples 1 and 5-6, it can be seen that the addition of silk protein composite microspheres and the amount added have a significant impact on skin texture and moisture content. Comparing Example 1 with Examples 4-7, it can be seen that when used in combination with skin conditioning agents and moisturizers, with the skin conditioning agents preferably being panthenol, allantoin, or silk amino acids, and the moisturizers preferably being squalane and meadowfoam seed oil, the functions of various active ingredients can be maximized.

[0154] Therefore, the moisturizing and anti-wrinkle cream provided by the present invention contains composite microspheres, which are absorbed through the skin. When applied to the cream formulation, they greatly improve the transdermal penetration efficiency of the cream.

[0155] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A moisturizing and anti-wrinkle cream, characterized in that, The raw materials for preparing the moisturizing and anti-wrinkle cream include, by weight percentage: Silk-core protein composite microsphere solution 2%~10% Skin conditioning agent 2%~5% Moisturizer 1%~2% Moisturizer 8%~15% Emulsifier 0.1%~1% Emulsion stabilizer 1%~2% Thickener 0.1%~0.2% The remainder is water.

2. The moisturizing and anti-wrinkle cream according to claim 1, characterized in that, The silk-core protein composite microsphere solution contains silk-core protein composite microspheres, which are prepared by the following method: silk-core protein and polydeoxyribonucleic acid are interpenetrated, entangled, folded and compressed under the action of polylysine to obtain the silk-core protein composite microspheres; Preferably, the average particle size of the silk-core protein composite microspheres is 50 nm to 500 nm; Preferably, the polylysine comprises basic polylysine and / or modified polylysine.

3. The moisturizing and anti-wrinkle cream according to claim 1 or 2, characterized in that, In the raw materials for preparing the silk core protein composite microspheres, the mass ratio of silk core protein to polylysine is 1:(0.006~0.2), the mass ratio of silk core protein to polydeoxyribonucleic acid is 1:(1~10), and the mass ratio of polylysine to polydeoxyribonucleic acid is 1:(5~150). Preferably, the mass percentage of silk core protein in the silk core protein composite microsphere solution is 1% to 5%; Preferably, the polylysine content in the silk-core protein composite microsphere solution is 0.01%~1% by mass; Preferably, the mass percentage of polydeoxyribonucleic acid in the silk-core protein composite microsphere solution is 1% to 5%; Preferably, the silk-core protein composite microsphere solution further contains a pH adjuster and a thickener.

4. The moisturizing and anti-wrinkle cream according to claim 1, characterized in that, The skin conditioning agent includes any one or a combination of at least two of the following: silk amino acids, panthenol, tetrahydromethylpyrimidine carboxylic acid, allantoin, glyceryl glucoside, or vinyl polydimethylsiloxane / polymethylsiloxane sesquisiloxane crosslinked polymer. Preferably, the skin conditioning agent is a combination of panthenol, allantoin, and silk amino acids, wherein the mass ratio of panthenol, allantoin, and silk amino acids is (1~30):(0.1~10):1; Preferably, the emollient comprises any one or a combination of at least two of glyceryl triethylhexanoate, squalane, or meadowfoam seed oil; Preferably, the emollient is a combination of squalane and meadowfoam seed oil; wherein the mass ratio of squalane to meadowfoam seed oil is 1:(0.1~1); Preferably, the moisturizer includes any one or a combination of at least two of erythritol, 1,3-propanediol, glycerin, tranexamic acid, trehalose, and 1,2-hexanediol.

5. The moisturizing and anti-wrinkle cream according to claim 1, characterized in that, The emulsifier includes polyglycerol-3-methylglucoside distearate; Preferably, the emulsifier stabilizer comprises a sodium acrylate / sodium acryloyl dimethyl taurate copolymer; Preferably, the thickener comprises an acrylate / C10-30 alkanol acrylate crosspolymer.

6. The moisturizing and anti-wrinkle cream according to claim 1, characterized in that, The repair cream also includes a pH adjuster; Preferably, the pH value of the moisturizing and anti-wrinkle cream is 4.0~8.5; Preferably, the pH adjuster is aminomethylpropanol.

7. A method for preparing a moisturizing and anti-wrinkle cream as described in any one of claims 1 to 6, characterized in that, The preparation method includes the following steps: The formula is prepared by mixing the silk protein composite microsphere solution, skin conditioning agent, emollient, moisturizer, emulsifier, emulsion stabilizer, thickener and water, stirring, and after sampling and testing to ensure it passes the test, it is then filled to obtain the cream.

8. The preparation method according to claim 7, characterized in that, The preparation steps are as follows: Phase A consists of panthenol, allantoin, erythritol, 1,3-propanediol, glycerol, trehalose, and acrylate / C10-30 alkanol acrylate crosspolymers. Phase B consisted of glycerol tri(ethylhexanoate), squalane, meadowfoam seed oil, and polyglycerol-3-methylglucose distearate. The C phase consists of a vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane crosslinked polymer and a sodium acrylate / sodium acryloyldimethyltaurate copolymer. Using aminomethylpropanol as phase D; The E phase consisted of silk core protein composite microsphere solution, tetrahydromethylpyrimidine carboxylic acid, glycerol glucoside, silk amino acids, tranexamic acid, and 1,2-hexanediol. (1) Add phase A to the emulsifying pot, homogenize, stir and heat to dissolve; Add phase B material to the oil pan, stir and heat to dissolve; (2) Maintain a vacuum state, transfer phase B in the oil pan to the emulsification pan, add phase C, emulsify and homogenize, and keep warm to defoam; (3) Cool down, add phase D and stir evenly, and continue to cool down; (4) Cool down and add phase E and stir until homogeneous; (5) Cool down to 30~38℃, stop stirring, and obtain the cream.

9. The preparation method according to claim 8, characterized in that, The dissolution temperature of phase A is 80℃~90℃; Preferably, the dissolution temperature of phase B is 80℃~90℃; Preferably, the temperature at which phase D is added is 70℃~80℃; Preferably, the temperature at which the E phase is added is 40℃~50℃.

10. The preparation method according to claim 7, characterized in that, The preparation method of the silk core protein composite microsphere solution is as follows: Add polydeoxyribonucleic acid to water and stir until homogeneous to obtain an aqueous solution of polydeoxyribonucleic acid; Dilute the silk protein solution, mix it with an aqueous solution of polydeoxyribonucleic acid, add an aqueous solution of polylysine to obtain a composite solution system, and stir to mix. Then add an optional pH adjuster and thickener, and stir to obtain the silk core protein composite microsphere solution.