Compound plant essential oil for eradicating helicobacter pylori and preparation method thereof

Abstract

The invention discloses compound plant essential oil for eradicating helicobacter pylori and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The plant essential oil comprises an active component and base oil, wherein the content of the active component is 15-20wt%; the active ingredients comprise the following components in parts by weight: 2-4 parts of sandalwood essential oil, 2-3 parts of patchouli oil, 1-3 parts of frankincense essential oil, 2-3 parts of ginger essential oil, 1-1.5 parts of liquorice essential oil, 2-3 parts of thyme essential oil and 1-2 parts of rhizoma bletillae essential oil. The compound plant essential oil provided by the invention can eradicate helicobacter pylori, and has the advantages of quick response, good curative effect and convenience in use.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, and more specifically, relates to a compound plant essential oil for eradicating Helicobacter pylori and its preparation method. Background Technology

[0002] Gastritis is a common chronic disease in clinical practice, mainly associated with Helicobacter pylori (Hp) infection. It is characterized by its difficulty in curing, frequent relapses, and long course. Clinically, quadruple therapy is commonly used to combat Hp infection and protect the gastric mucosa. However, this regimen has certain toxic side effects and also eradicates beneficial bacteria, making it not the optimal treatment option and hindering gastritis recovery. Clinically, there is an urgent need for treatment methods with better efficacy and fewer side effects. my country has a long history of using aromatherapy techniques. Modern aromatherapy extracts small-molecule substances from herbal plants, which have antibacterial, anti-inflammatory, and gastric mucosa-protective effects. They also have a bidirectional antibacterial regulatory effect, protecting the gastrointestinal flora. They are easily absorbed, fast-acting, and have no toxic side effects. Through human smell and skin absorption, they are excreted via the skin and urinary tract, bypassing liver metabolism. They regulate the neuroendocrine system, acting on target points to achieve therapeutic goals.

[0003] Korona-Glowniak et al. studied the anti-Helicobacter pylori (H. pylori) effects of 26 essential oils and found that lemongrass, cedarwood, thyme, lemon balm, and basil essential oils had the highest antibacterial activity. Knezevic et al. found that creeping thyme essential oil had antibacterial activity against H. pylori, with a MIC of 2.0–4.0 μl / ml. Preuss et al. studied the antibacterial activity of 13 essential oils against H. pylori and showed that lemon verbena and lemongrass had the highest antibacterial activity. Bergonzelli et al. studied the antibacterial activity of 60 essential oils against Helicobacter pylori and found that 30 essential oils could affect the growth of H. pylori in vitro, and 15 essential oils had strong antibacterial activity. Among the components of these essential oils, carvacrol, isoeugenol, nerol, citral, and sapindus mucilage had the strongest anti-H. pylori activity. Ohno et al. studied the antibacterial effects of 13 plant essential oils against clinically isolated and ATCC-isolated Helicobacter pylori. They found that tea tree, Mediterranean cypress, European juniper, lemon verbena, basil, blue gum, peppermint, sweet oregano, aromatic ravensa leaf, lemon, broadleaf lavender, lemongrass, and rosemary essential oils showed antibacterial activity against all tested strains. Compared to untreated mice, lemongrass essential oil significantly reduced the number of Helicobacter pylori in the mice's stomachs. Hartmani et al. studied the anti-Helicobacter pylori activity of a 2:1 mixture of summer peppermint and oregano essential oils, finding that this mixture successfully eradicated Helicobacter pylori in 70% of mice.

[0004] Currently, there are no reports on the external application of compound plant essential oils to eradicate Helicobacter pylori. Summary of the Invention

[0005] Based on the aforementioned technical problems in the existing technology, the purpose of this invention is to provide a compound plant essential oil for eradicating Helicobacter pylori and its preparation method.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows:

[0007] A compound plant essential oil for eradicating Helicobacter pylori, comprising active ingredients and base oil, wherein the content of the active ingredients is 15-20 wt%; by weight, the active ingredients include: 2-4 parts sandalwood essential oil, 2-3 parts patchouli oil, 1-3 parts frankincense essential oil, 2-3 parts ginger essential oil, 1-1.5 parts licorice essential oil, 2-3 parts thyme essential oil, and 1-2 parts Bletilla striata essential oil.

[0008] Furthermore, the content of the active ingredient is 15 wt%.

[0009] Furthermore, the base oil is sweet almond oil.

[0010] The preparation method of the above-mentioned compound plant essential oil includes the following steps:

[0011] Weigh each active ingredient according to the formula, mix and stir, allow to settle, take the upper layer of oil and remove the bottom impurities; add base oil according to the ratio, mix and stir again to obtain the final product.

[0012] Sandalwood essential oil contains beta-terpenes, beta-terpene alcohols, and santalin, which inhibit the colonization enzyme activity of Helicobacter pylori, inhibit the replication of Helicobacter pylori genes, and destroy the cell wall of Helicobacter pylori.

[0013] Patchouli essential oil: contains terpenes and patchouli alcohol, which inhibit Helicobacter pylori colonization and interfere with Helicobacter pylori colonization enzymes; it also enhances gastric motility and aids digestion.

[0014] Frankincense essential oil: contains α-terpinene, limonene, myrrhene, and esters; it has antibacterial and anti-inflammatory properties, repairs the gastric mucosa, and enhances immune function.

[0015] Ginger essential oil: contains gingerol, gingerol, and monoterpenes, which have antibacterial and antimicrobial properties, and can dispel cold and warm the stomach.

[0016] Licorice essential oil: contains glycyrrhizic acid and glycyrrhizin, which have antibacterial and anti-inflammatory properties, inhibit the colonization enzyme activity of Helicobacter pylori, inhibit the replication of Helicobacter pylori genes, destroy the cell wall of Helicobacter pylori, and enhance the immune system.

[0017] Thyme essential oil contains thymol, linalool, and borneol, which inhibit the colonization enzyme activity of Helicobacter pylori, inhibit the replication of Helicobacter pylori genes, and destroy the cell wall of Helicobacter pylori.

[0018] Bletilla striata essential oil: contains Bletilla striata polysaccharides, which have astringent, hemostatic, anti-inflammatory, and tissue-regenerating effects, and can repair the gastric mucosa.

[0019] Sweet almond oil: has a diluent effect.

[0020] The compound plant essential oils are absorbed transdermally, with some reaching the gastric mucosa via blood circulation and others directly penetrating it. A third portion activates the gastric nerve through olfaction, regulating endocrine metabolism through neural modulation. Therefore, the compound plant essential oils in this invention eradicate Helicobacter pylori through multiple pathways.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0022] 1. The compound plant essential oil of this invention has a high inhibitory effect on *Helicobacter pylori* (Hp), with a minimum inhibitory concentration (MIC) of 256 μg / mL. Animal experiments have shown that it has a good inhibitory effect on Hp and can significantly improve gastric diseases. Using the compound plant essential oil can reduce the use of antibiotics, maintain intestinal flora, and avoid the toxic side effects of traditional quadruple therapy.

[0023] 2. The compound plant essential oil of this invention rarely develops drug resistance and has a bidirectional regulatory effect. It is easy to use, fast-acting, and can eradicate Helicobacter pylori with simple external application.

[0024] 3. The essential oil components in the compound plant essential oil of this invention are small molecules, which are easily absorbed, have strong universality, and are convenient and quick for external use. Attached Figure Description

[0025] Figure 1 HE staining of gastric mucosal tissue from each group of rats in test case 4 (HE, ×400).

[0026] Figure 2 The results of the compound plant essential oil in Example 4 on the expression levels of IL-6 (A), IL-1β (B), and TNF-α (C) in the gastric mucosa of rats in each group (x¯±s, n=6);

[0027] Note: "#" indicates P < 0.05 compared with the control group, "##" indicates P < 0.01 compared with the control group; "*" indicates P < 0.05 compared with the model group, "**" indicates P < 0.01 compared with the model group, and so on.

[0028] Figure 3 To test the effect of compound plant essential oil in Example 4 on the relative expression levels of TLR4 (A), MyD88 (B), and NF-κB P65 mRNA (C) in the gastric mucosa of rats in each group (x¯±s, n=3).

[0029] Figure 4 To test the effects of the compound plant essential oil in Example 4 on the expression of TLR4 (B), MyD88 (C), and NF-κBP65 (D) proteins in rat gastric mucosa,

[0030] In the figure, A is a protein electrophoresis image, a is the blank group, b is the model group, c is the low-dose stomach-nourishing oil group, d is the high-dose stomach-nourishing oil group, and e is the standard quadruple therapy group. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be described in further detail below with reference to embodiments, but the embodiments of this invention are not limited thereto. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to limit the invention. It should be noted that, unless otherwise specified, the reagents and other materials used in this embodiment are all common commercially available products.

[0032] Directions for use: Take 5 drops each time and apply evenly to the area below the xiphoid process (chest hollow), about the size of your palm, until there is no visible liquid on the skin surface. Use 4 times a day. Do not drink alcohol or eat cold, raw, or spicy foods during use.

[0033] Example 1

[0034] A compound plant essential oil for eradicating Helicobacter pylori, composed of active ingredients and base oil, with the content of active ingredients being 15wt%; by weight, the active ingredients are: 3 parts sandalwood essential oil, 2 parts patchouli oil, 2 parts frankincense essential oil, 3 parts ginger essential oil, 1 part licorice essential oil, 2 parts thyme essential oil, and 1 part Bletilla striata essential oil; the base oil is sweet almond oil.

[0035] It is prepared by the following method: mix the active ingredients, stir and let stand, take the supernatant and add it to the base oil, mix evenly, and it is ready.

[0036] Example 2

[0037] A compound plant essential oil for eradicating Helicobacter pylori, composed of active ingredients and base oil, with the active ingredients content being 20wt%; by weight, the active ingredients are: 4 parts sandalwood essential oil, 3 parts patchouli oil, 1 part frankincense essential oil, 2 parts ginger essential oil, 1.5 parts licorice essential oil, 3 parts thyme essential oil, and 2 parts Bletilla striata essential oil; the base oil is sweet almond oil.

[0038] It is prepared by the following method: weigh each active ingredient according to the formula, mix and stir, precipitate, take the upper oil and remove the bottom impurities; add base oil according to the ratio, mix and stir again to obtain the product.

[0039] Example 3

[0040] A compound plant essential oil for eradicating Helicobacter pylori, composed of active ingredients and base oil, with the content of active ingredients being 18wt%; by weight, the active ingredients are: 2 parts sandalwood essential oil, 3 parts patchouli oil, 1 part frankincense essential oil, 3 parts ginger essential oil, 1.5 parts licorice essential oil, 2 parts thyme essential oil, and 1 part Bletilla striata essential oil.

[0041] It is prepared by the following method: weigh each active ingredient according to the formula, mix and stir, precipitate, take the upper oil and remove the bottom impurities; add base oil according to the ratio, mix and stir again to obtain the product.

[0042] Performance testing

[0043] The compound plant essential oil prepared using the examples was tested using urea respiration experiments conducted by volunteers at Hunan Water Conservancy Hospital. 13 C was used to detect Hp before and after application.

[0044] A DOB value ≥ 4.0 (positive) indicates that the subject is infected with Helicobacter pylori; a DOB value < 4.0 (negative) indicates that the subject does not have Helicobacter pylori, and in this case, it means that Helicobacter pylori has been eradicated.

[0045] The application method is as follows: Apply 5 drops to the upper abdomen 4 times a day for 4 consecutive weeks, and have a follow-up examination 30 days after stopping treatment. 13 C. Assess the gut microbiota and clinical symptoms. During the observation period, strictly limit irritating foods, alcohol, cold foods, and other unhealthy lifestyle habits.

[0046] Test Example 1

[0047] The subjects underwent urea respiration on the same day. 13 The C test was positive. Starting that day, only the compound plant essential oil prepared as described in Example 1 was applied, without any other intervention. After 10 days of applying the plant essential oil, 4 of the 6 patients showed improvement. 13 C DOB value <4.0 (negative), 2 cases 13 C DOB value ≥ 4.0 (positive); apply plant essential oil for 30 days, then stop for 30 days, 6 patients 13 C DOB values ​​were all <4.0 (negative). Results showed that, according to the expert consensus criteria for Helicobacter pylori quadruple therapy, Helicobacter pylori was eradicated in all 6 patients in this case group.

[0048] Table 1. Plant essential oils for eradicating Helicobacter pylori 13 Results of C on the day before, 10 days before, and 60 days after the test.

[0049]

[0050] As shown in Table 1, the compound plant essential oil provided by this invention is fast-acting. After ten days of application, it can basically eliminate Helicobacter pylori in patients with mild symptoms and also eliminate most of Helicobacter pylori in patients with severe symptoms. After completing the entire course of treatment for 30 days, there was no rebound, indicating that the compound plant essential oil can eradicate Helicobacter pylori.

[0051] Test Example 2

[0052] Subjects were performing urea respiration. 13 After a positive C test, no antibiotics or other traditional Chinese medicines were taken. During the treatment period, only the compound plant essential oil prepared in Example 2 was applied, without any other intervention. The plant essential oil was applied for 30 days, then stopped for another 30 days. (15 patients) 13 C DOB values ​​were all <4.0 (negative). Results showed that, according to the expert consensus criteria for Helicobacter pylori quadruple therapy, Helicobacter pylori was eradicated in all 15 patients in this case group.

[0053] Table 2. Plant essential oils for eradicating Helicobacter pylori 13 Results before and 60 days after test C

[0054]

[0055] As shown in Table 2, the compound plant essential oil prepared in the embodiments of the present invention can eradicate Helicobacter pylori and has good efficacy for patients of all ages, with advantages such as strong universality and ease of use. Figure 1 and Figure 2 After intervention with the compound plant essential oil of the present invention, Helicobacter pylori can be significantly eradicated.

[0056] Test Example 3: In vitro antibacterial test of compound plant essential oils

[0057] Experimental strain: Helicobacter pylori standard strain NCTC1639, provided by the Microbiology Research Laboratory of Guizhou Medical University. The compound plant essential oil was prepared according to Example 1. MH(A) medium and phosphate-buffered saline (1xPBS) were purchased from Wuhan Sewell Biotechnology Co., Ltd., 0.9% physiological saline (NS) was purchased from Guizhou Kelun Pharmaceutical Co., Ltd., and anhydrous ethanol was purchased from Chengdu Jinshan Chemical Reagent Co., Ltd.

[0058] Test method:

[0059] 1. Preparation of MH(A) medium

[0060] Weigh 3.65 g of MH(A) culture medium and dissolve it in 100 mL of RO water (deionized water). Autoclave at 121℃ for 15 minutes and set aside.

[0061] 2. Preparation of egg liquid

[0062] After sterilizing the eggshell, aseptically mix the egg yolk with sterile 1xPBS at a 1:1 (volume ratio) to prepare an egg solution for later use.

[0063] 3. Prepare the compound plant essential oil stock solution (10 mg / mL)

[0064] Weigh 10 mg of the compound plant essential oil and dissolve it in 1 mL of anhydrous ethanol. After it is fully dissolved, store it in a refrigerator at 2-8℃.

[0065] 4. Prepare various dilution ratios of the compound plant essential oils (prepare immediately before use).

[0066] Ten sterile 1.5 mL EP tubes were used, each labeled with a different dilution concentration. The stock solution was diluted with anhydrous ethanol to a concentration of 5120 μg / mL. Then, using anhydrous ethanol as the diluent, the solution was diluted twofold to obtain the following concentration gradients: 5120 μg / mL, 2560 μg / mL, 1280 μg / mL, 640 μg / mL, 320 μg / mL, 160 μg / mL, 80 μg / mL, 40 μg / mL, 20 μg / mL, and 10 μg / mL.

[0067] 5. Preparation of MH(A) Egg Culture Medium

[0068] Remove the autoclaved MH(A) medium and cool it to approximately 55-60°C. Add 12 mL of egg liquid to every 100 mL of MH(A) medium and mix thoroughly.

[0069] 6. Prepare drug-containing culture medium plates and control plates.

[0070] Add 50 μL of each of the above dilutions of compound plant essential oil to each well of a 24-well or 48-well plate, so that the final concentrations of the compound plant essential oil are: 256 μg / mL, 128 μg / mL, 64 μg / mL, 32 μg / mL, 16 μg / mL, 8 μg / mL, 4 μg / mL, 2 μg / mL, 1 μg / mL, and 0.5 μg / mL, respectively.

[0071] Each compound plant essential oil final concentration was set up in triplicate; simultaneously, NS / PBS control, anhydrous ethanol control (anhydrous ethanol antibacterial control, anhydrous ethanol sterile control), culture medium control (denoted as B), and bacterial growth control (two groups, denoted as B1 and B2) were also set up, with each control having three replicates and each well having a volume of 1 mL. The preparation methods for each group are as follows:

[0072] Compound plant essential oil group: 50 μL compound plant essential oil + 950 μL MH(A) egg culture medium.

[0073] Anhydrous ethanol control group: 50 μL anhydrous ethanol + 950 μL MH(A) egg culture medium.

[0074] NS / PBS control group, culture medium control group, bacterial growth control group: 1 mL MH(A) egg culture medium.

[0075] 7. Prepare bacterial culture (prepare immediately before use)

[0076] Take fresh culture from S7 egg culture medium and prepare a McFarland 4.0 bacterial suspension using sterile normal saline (NS) or PBS. (Note: In the three replicate experiments, sterile PBS was used to prepare the bacterial suspension in the first experiment, while sterile normal saline was used in the second and third experiments.)

[0077] 8. Inoculation solution

[0078] For each dilution of compound plant essential oil wells, anhydrous ethanol antibacterial control, and bacterial growth control, add 10 μL of bacterial solution to each well. (Note: The order of adding bacterial solution is: B1 → each dilution of compound plant essential oil wells → anhydrous ethanol antibacterial control → B2).

[0079] NS / PBS control: Add 10 μL of NS or PBS to each well.

[0080] Culture medium control, anhydrous ethanol sterile control: no bacterial solution or NS / PBS added.

[0081] 9. Cultivation and Observation Results

[0082] Incubate the above-mentioned drug-containing culture medium (24 or 48 wells) with bacterial solution in a 37°C, 10% CO2 incubator for 72 hours. After bacterial growth is observed in controls B1 and B2 wells, prepare the oxidase reagent (prepare fresh, dissolve 0.001 g of oxidase powder in 100 μL of sterile water). Add 10 μL of the oxidase reagent to each well. A blue-purple color within 2 minutes indicates a positive oxidase test, signifying bacterial growth.

[0083] The experimental results are shown in Table 3. After adding the oxidase reagent, both the bacterial growth control group and the anhydrous ethanol control (5%) were observed to be positive (i.e., bacterial growth was observed), indicating that 5% anhydrous ethanol had no significant antibacterial effect. However, the compound plant essential oil group showed negative results (i.e., no bacterial growth) at a concentration of 256 μg / mL, indicating that the minimum inhibitory concentration (MIC) of the compound plant essential oil against Helicobacter pylori was 256 μg / mL.

[0084] Table 3. MIC determination results of compound plant essential oils against Helicobacter pylori S7 strain

[0085]

[0086] Note:

[0087] "+" indicates a positive oxidase test (i.e., bacterial growth).

[0088] "-" indicates a negative oxidase test (i.e., no bacterial growth).

[0089] " / " indicates that the repeat hole was not made;

[0090] B1 and B2 indicate inoculation of bacterial suspension into drug-free culture medium, with the purpose of determining whether the bacteria in the prepared bacterial suspension are active.

[0091] Test Example 4: Inhibition of Helicobacter pylori-associated gastritis in rats by compound plant essential oils

[0092] Construction of rat model: Fifty SD rats weighing 25-30g were randomly divided into 5 groups (blank group, model group, low-dose compound plant essential oil group, high-dose compound plant essential oil group, and standard quadruple therapy group) according to sex, with 10 rats in each group. After grouping, all rats were given antibiotics by gavage for 7 days. A rapid urease test was performed, and all 50 rats were negative.

[0093] The model group, the low-dose compound plant essential oil group, the high-dose compound plant essential oil group, and the standard quadruple therapy group were then administered Helicobacter pylori standard strain (NCTC1639) by gavage for 2 weeks. A rapid urease test was performed, and all 40 mice tested positive, confirming the successful modeling of Helicobacter pylori infection in SD mice. The control group underwent no treatment after gastrointestinal sterilization, did not receive any Helicobacter pylori strain gavage, and were fed normally.

[0094] Administration method

[0095] Low-dose compound plant essential oil group: Each time, 0.05 ml of compound plant essential oil was applied to the area below the xiphoid process of SD mice, with an application area of ​​about 1 cm in diameter. The application was repeated 4 times a day for 30 consecutive days. After each application, the application area was covered with gauze.

[0096] High-dose compound plant essential oil group: Each time, 0.1 ml of compound plant essential oil was applied to the area below the xiphoid process of SD mice, with an application area of ​​about 1 cm in diameter. The application was carried out 4 times a day for 30 consecutive days. After each application, the application area was covered with gauze.

[0097] Standard quadruple therapy group: Based on body weight: omeprazole, 20-40 mg / kg, once daily; amoxicillin, 200 mg / kg, twice daily; clarithromycin (50-100 mg / kg, twice daily); bismuth potassium citrate, 50-100 mg / kg, twice daily. All medications were administered by gavage (1-2 mL syringe for mice), and administration was discontinued after 14 consecutive days.

[0098] Model group: No treatment is given; only feed and water are provided.

[0099] Thirty days after drug withdrawal in the compound herbal essential oil group and 45 days after drug withdrawal in the standard quadruple therapy group, a rapid urease test was performed on all 50 SD rats in the five groups on the same day. All rats were then euthanized by dislocation, and gastric tissue was collected. Hematoxylin-eosin (HE) staining was used to observe pathological changes in the gastric mucosa of each group, the effects of the compound herbal essential oil on the levels of IL-6, IL-1β, and TNF-α in the rat gastric mucosa, the effects of the compound herbal essential oil on the expression of TLR4, MyD88, and NF-κB P65 mRNA in the rat gastric mucosa, and the effects of the compound herbal essential oil on the protein expression of TLR4, MyD88, and NF-κB P65 in the rat gastric mucosa. Multi-omics sequencing was also performed, including RT-PCR, transcriptomics, and protein sequencing.

[0100] Table 4 shows that the low-dose compound plant essential oil group had a 30% eradication rate of *H. pylori*, while the high-dose group had a 60% eradication rate; the standard quadruple therapy group had an 80% eradication rate. Rapid urease test results indicate that the low- and high-dose compound plant essential oil groups, as well as the standard quadruple therapy group, all had a certain degree of effectiveness in eradicating *H. pylori*.

[0101] Table 4. *H. pylori* eradication rate in rats of each group (n=10)

[0102]

[0103] like Figure 1 As shown, compared with the blank group, the gastric mucosa of rats in the model group was significantly damaged after infection with Helicobacter pylori. Compared with the model group, the pathological damage of gastric mucosa in rats in the low- and high-dose compound plant essential oil intervention groups and the standard quadruple therapy group was improved to varying degrees.

[0104] like Figure 2 As shown, compared with the blank group, the levels of IL-6, IL-1β, and TNF-α in the gastric mucosa of rats in the model group were significantly increased (P<0.01). Compared with the model group, the levels of IL-6, IL-1β, and TNF-α in the gastric mucosa of rats in the low-dose compound plant essential oil group, the high-dose compound plant essential oil group, and the standard quadruple therapy group were significantly decreased (P<0.01).

[0105] like Figure 3 and Figure 4As shown, compared with the blank group, the relative expression levels of TLR4, MyD88, and NF-κBP65 mRNA and their corresponding protein expression in the gastric mucosa of rats in the model group were significantly increased (P<0.05 or P<0.01). Compared with the model group, the relative expression levels of TLR4, MyD88, and NF-κBP65 mRNA and their protein expression in the gastric mucosa of rats in the low-dose compound plant essential oil group, the high-dose compound plant essential oil group, and the standard quadruple therapy group were significantly decreased (P<0.05 or P<0.01).

[0106] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A compound plant essential oil for eradicating Helicobacter pylori, characterized in that, It includes active ingredients and base oil, wherein the content of the active ingredients is 15-20 wt%; The active ingredients, by weight, include: 2-4 parts sandalwood essential oil, 2-3 parts patchouli oil, 1-3 parts frankincense essential oil, 2-3 parts ginger essential oil, 1-1.5 parts licorice essential oil, 2-3 parts thyme essential oil, and 1-2 parts bletilla essential oil.

2. The compound plant essential oil according to claim 1, characterized in that, The content of the active ingredient is 15 wt%.

3. The compound plant essential oil according to claim 1, characterized in that, The base oil is sweet almond oil.

4. The method for preparing the compound plant essential oil according to any one of claims 1-3, characterized in that, Includes the following steps: Weigh each ingredient according to the formula, mix and stir, let it settle, take the upper layer of oil and remove the bottom impurities; add the base oil according to the ratio, mix and stir again to obtain the final product.

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