Compound antibacterial spray for pets as well as preparation method and application of compound antibacterial spray
This compound pet antibacterial spray, made by loading multiple antibacterial ingredients onto modified mesoporous silica, solves the problems of slow treatment speed and poor durability of existing single-component antibacterial products, achieving long-lasting antibacterial effects and good stability, and is suitable for the treatment of pet skin diseases.
Patent Information
- Application Number
- CN202511462285.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-14
- Publication Date
- 2026-01-09
AI Technical Summary
Existing single-component antibacterial products are slow to treat pet skin diseases and have poor antibacterial durability, resulting in mediocre efficacy. Furthermore, pet skin diseases are prone to mixed infections and recurrence.
Using D-cysteine and/or polyethyleneimine-modified mesoporous silica as a carrier, chlorhexidine gluconate, miconazole nitrate, ivermectin, and jojoba oil are loaded to form a compound pet antibacterial spray. It is prepared by ultrasonic mixing and vacuum drying to achieve multi-layer loading and coating. Combined with the use of carboxymethyl cellulose solution, the antibacterial effect and stability are improved.
It achieves long-lasting antibacterial function, has good storage stability, and its antibacterial performance declines less under strong light irradiation. The combination of chlorhexidine gluconate, miconazole nitrate, and ivermectin significantly improves the antibacterial performance and has high safety.
Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the technical field of pet medicine, and particularly relates to a compound pet bacteriostatic spray and a preparation method and application thereof. BACKGROUND
[0002] Pet skin disease is one of the most common diseases of pets. A series of reasons such as contact with other pets with skin diseases, too frequent bathing, and failure to timely deworming can cause pet skin disease. Generally, pet skin disease is divided into eight categories, including ectoparasitic skin disease, endocrine disorder alopecia, folliculitis, ear mite, pyoderma, eczema, fungal infection, and scabies mite. Pets with skin disease will have itching, hair loss, and pus formation at the affected site, and can also cause other inflammations to cause fever in pets. Some pet skin diseases can also be transmitted to pet owners.
[0003] At present, the main treatments for pet skin disease are medicated bath or skin spray, tablets, etc., such as terbinafine hydrochloride spray, terbinafine hydrochloride tablets, and zinc oxide ointment. However, pet skin disease is prone to mixed infection and repeated occurrence. Single bacteriostatic product has slow treatment speed and poor bacteriostatic durability, resulting in general curative effect. Based on this problem, the application provides a compound pet bacteriostatic spray and a preparation method and application thereof. SUMMARY
[0004] The application aims to solve the above problems and provide a compound pet bacteriostatic spray and a preparation method and application thereof.
[0005] The application achieves the above-mentioned purpose through the following technical scheme: The application provides a compound pet bacteriostatic spray, which comprises the following preparation raw materials in mass percentage: chlorhexidine gluconate 3-7%, miconazole nitrate 3-8%, ivermectin 3-8%, glycerol 5-10%, carboxymethyl cellulose solution 2-8%, modified mesoporous silica 12-18%, jojoba oil 5-10%, Tween 80 0.1-2%, oil-soluble azone 1-3%, anhydrous ethanol 8-15%, and purified water 35-45%. The modified mesoporous silica is obtained after modification by D-cysteine and / or polyethyleneimine.
[0006] As a further optimization scheme of the application, the compound pet bacteriostatic spray comprises the following preparation raw materials in mass percentage: chlorhexidine gluconate 5%, miconazole nitrate 5%, ivermectin 5%, glycerol 8%, carboxymethyl cellulose solution 6%, modified mesoporous silica 14%, jojoba oil 7%, Tween 80 1%, oil-soluble azone 2%, anhydrous ethanol 12%, and purified water 35%.
[0007] As a further optimization of the present invention, the method for obtaining the modified mesoporous silica is as follows: the mesoporous silica is dispersed in an 8-12 mg / mL D-cysteine solution, stirred at 35-45°C for 12-18 h, then 0.1-1.5 g / mL polyethyleneimine solution is added, and the mixture is heated in a water bath at 40-45°C for 2-3 h, during which time it is stirred at a speed of 80-100 r / min. The mixture is then washed with pure water, filtered, and dried to obtain the modified mesoporous silica.
[0008] As a further optimization of the present invention, the mesoporous silicon has a particle size of 200-300 nm.
[0009] As a further optimization of the present invention, the mass ratio of the mesoporous silica to the 8-12 mg / mL L-cysteine solution is 1:3-5; The mass ratio of the mesoporous silica to the 0.1-1.5 g / mL polyethyleneimine solution is 1:5-10.
[0010] A method for preparing a compound antibacterial spray for pets, the specific steps of which are as follows: (1) Chlorhexidine gluconate, miconazole nitrate, ivermectin and modified mesoporous silica were ultrasonically mixed, and then half of the total amount of carboxymethyl cellulose solution was added. Ultrasonic mixing was continued, and the load material was obtained after vacuum drying. (2) Load material one is ultrasonically mixed with jojoba oil, oil-soluble azone and Tween 80, and then the remaining amount of carboxymethyl cellulose solution is added. Ultrasonic mixing is continued, and after vacuum drying, load material two is obtained. (3) The loading material II was ultrasonically mixed with glycerol, anhydrous ethanol and purified water to obtain a compound pet antibacterial spray.
[0011] As a further optimization of the present invention, in step one, the power of the ultrasonic mixing treatment is 300-400W, the time is 30-40min, and the temperature is 20-40℃; In step two, the ultrasonic mixing treatment power is 100-150W, the time is 20-30min, and the temperature is 30-45℃; In step three, the ultrasonic mixing treatment has a power of 500-700W, a time of 20-30min, and a temperature of 20-25℃.
[0012] The use of a compound pet antibacterial spray as described above in the preparation of medications for treating or improving pet skin diseases.
[0013] As a further optimization of the present invention, the topical dosage of the compound pet antibacterial spray is 1-3 times a day, with each application being 0.02-1g / kg.
[0014] The beneficial effects of this invention are as follows: This invention utilizes modified mesoporous silica prepared by D-cysteine and / or polyethyleneimine modification to load chlorhexidine gluconate, miconazole nitrate, ivermectin, jojoba oil, Tween 80, and oil-soluble azone in a multilayer configuration, and then coats it with carboxymethyl cellulose. This mixture is then combined with anhydrous ethanol and purified water to obtain a compound pet antibacterial spray. This compound pet antibacterial spray achieves long-lasting antibacterial function and exhibits good storage stability. Under strong light irradiation, its antibacterial performance shows minimal degradation. Furthermore, the combination of chlorhexidine gluconate, miconazole nitrate, and ivermectin may have a synergistic effect. While their individual antibacterial properties are relatively poor, their combined use significantly improves their sustained antibacterial performance. Detailed Implementation
[0015] The present application will now be described in further detail. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.
[0016] I. Materials 1. Mesoporous silica: Particle size 200-300 nm, specific surface area 573±20 m² 2 g -1 The pore volume is 0.8 ± 0.05 cm. 3 g -1 ; 2. Chlorhexidine gluconate: Purchased from Wuhan Xinxin Jiali Biotechnology Co., Ltd., purity 99%; 3. Miconazole nitrate: Purchased from Shanghai Yuanye Biotechnology Co., Ltd., purity 98.5%; 4. Ivermectin: Purchased from Jiangsu Yiheng Pharmaceutical Co., Ltd., purity 99%; 5. D-cysteine: Purchased from Hebei Qiansheng Biotechnology Co., Ltd., purity 99%; 6. Polyethyleneimine: Purchased from Nantong Runfeng Petrochemical Co., Ltd., purity 99%; 7. Carboxymethyl cellulose solution: Carboxymethyl cellulose was purchased from Hubei Xinmingtai Chemical Co., Ltd., with a purity of 98%; it was dissolved in deionized water to obtain a carboxymethyl cellulose solution with a mass concentration of 5-8%. In the following examples, a carboxymethyl cellulose solution with a mass concentration of 6.8% was selected.
[0017] Unless otherwise specified, the methods used in this invention are conventional methods known to those skilled in the art, and the raw materials and reagents used are commercially available products.
[0018] II. Methods Example 1 (1) Preparation of modified mesoporous silica Mesoporous silica was dispersed in a 10 mg / mL D-cysteine solution at a mass ratio of 1:4 and stirred at 38 °C for 14 h; then washed with pure water, filtered and dried to obtain modified mesoporous silica.
[0019] (2) Preparation of compound pet antibacterial spray In this embodiment, the compound pet antibacterial spray comprises the following raw materials by mass percentage: chlorhexidine gluconate 7%, miconazole nitrate 3%, ivermectin 4%, glycerin 6%, carboxymethyl cellulose solution 2%, modified mesoporous silica 12%, jojoba oil 6%, Tween 80 0.5%, oil-soluble azone 1%, anhydrous ethanol 15%, and purified water 43.5%.
[0020] The specific steps of the preparation method are as follows: 2.1 Chlorhexidine gluconate, miconazole nitrate, ivermectin, and modified mesoporous silica were ultrasonically mixed at a power of 400W for 40 minutes at a temperature of 23℃. Then, half of the total amount of carboxymethyl cellulose solution was added, and ultrasonic mixing was continued at a power of 300W for 30 minutes at a temperature of 35℃. After vacuum drying, the first load material was obtained. 2.2 The loading material one was ultrasonically mixed with jojoba oil, oil-soluble azone, and Tween 80 at a power of 150W for 30 minutes and a temperature of 45℃. Then, the remaining carboxymethyl cellulose solution was added, and ultrasonic mixing was continued at a power of 100W for 20 minutes and a temperature of 30℃. After vacuum drying, the loading material two was obtained and stored at room temperature for later use. 2.3 After ultrasonically mixing the load material II with glycerol, anhydrous ethanol and purified water (ultrasonic mixing power of 550W, time of 25min, temperature of 23℃), a compound pet antibacterial spray is obtained.
[0021] Example 2 In this embodiment, the formulation of the compound pet antibacterial spray was adjusted. After adjustment, the compound pet antibacterial spray includes the following raw materials in the following mass percentages: chlorhexidine gluconate 5%, miconazole nitrate 5%, ivermectin 5%, glycerin 8%, carboxymethyl cellulose solution 6%, modified mesoporous silica 14%, jojoba oil 7%, Tween 80 1%, oil-soluble azone 2%, anhydrous ethanol 12%, and purified water 35%.
[0022] The preparation methods for modified mesoporous silica and compound pet antibacterial spray are consistent with those in Example 1.
[0023] Example 3 In this embodiment, the formulation of the compound pet antibacterial spray was adjusted. After adjustment, the compound pet antibacterial spray includes the following raw materials in the indicated mass percentages: chlorhexidine gluconate 4%, miconazole nitrate 3%, ivermectin 5%, glycerin 8%, carboxymethyl cellulose solution 8%, modified mesoporous silica 12%, jojoba oil 10%, Tween 80 1.2%, oil-soluble azone 1.8%, anhydrous ethanol 10%, and purified water 37%.
[0024] The preparation methods for modified mesoporous silica and compound pet antibacterial spray are consistent with those in Example 1.
[0025] Based on Example 2, this invention also explores technical solutions for preparing modified mesoporous silica using different modifying reagents, as detailed below: Example 4 In this embodiment, polyethyleneimine is used to modify mesoporous silica, as detailed below: The preparation of modified mesoporous silica is as follows: Mesoporous silica was dispersed in a 1.2 g / mL polyethyleneimine solution at a mass ratio of 1:7, heated in a water bath at 42 °C for 2.5 h with stirring at 85 r / min during the heating process, and then washed with pure water, filtered and dried to obtain modified mesoporous silica.
[0026] The preparation of the compound pet antibacterial spray is consistent with that in Example 2.
[0027] Example 5 In this embodiment, D-cysteine and polyethyleneimine are used to modify mesoporous silica sequentially, as detailed below: The preparation of modified mesoporous silica is as follows: 1.1. Disperse mesoporous silica in a 10 mg / mL D-cysteine solution at a mass ratio of 1:4 and stir at 38°C for 14 h; 1.2 Add 1.2 g / mL polyethyleneimine solution to the mixture in step 1.1 at a mass ratio of 1:7 (mass ratio of mesoporous silica to 1.2 g / mL polyethyleneimine solution), heat in a water bath at 42°C for 2.5 h, stirring at 85 r / min during the process, then wash with pure water, filter and dry to obtain modified mesoporous silica.
[0028] The preparation of the compound pet antibacterial spray is consistent with that in Example 2.
[0029] Example 6 In this embodiment, mesoporous silica is modified sequentially with polyethyleneimine and D-cysteine, as detailed below: The preparation of modified mesoporous silica is as follows: 1.1. Disperse mesoporous silica in a 1.2 g / mL polyethyleneimine solution at a mass ratio of 1:7, and heat in a water bath at 42°C for 2.5 h, stirring at 85 r / min during the process; 1.2. In the mixture of step 1.1, disperse the modified mesoporous silica in a 10 mg / mL D-cysteine solution at a mass ratio of 1:4 (mass ratio of mesoporous silica to 10 mg / mL D-cysteine solution), stir at 38°C for 14 h, then wash with pure water, filter and dry to obtain modified mesoporous silica.
[0030] The preparation of the compound pet antibacterial spray is consistent with that in Example 2.
[0031] Based on Example 5, this invention also explores technical solutions for compound pet antibacterial sprays prepared by different methods, as detailed below: Example 7 In this embodiment, the formulation of the compound pet antibacterial spray was adjusted. After adjustment, the compound pet antibacterial spray includes the following raw materials in the following mass percentages: chlorhexidine gluconate 5%, miconazole nitrate 5%, ivermectin 5%, glycerin 8%, carboxymethyl cellulose solution 6%, modified mesoporous silica 14%, jojoba oil 7%, Tween 80 1%, oil-soluble azone 2%, anhydrous ethanol 12%, and purified water 35%.
[0032] The preparation of the compound pet antibacterial spray is as follows: 1.1 Chlorhexidine gluconate, miconazole nitrate, ivermectin, jojoba oil, oil-soluble azone, Tween 80, and modified mesoporous silica were ultrasonically mixed. The ultrasonic mixing process consisted of two stages: the first stage used 400W of power for 40 minutes at 23°C; the second stage used 150W of power for 30 minutes at 45°C. Then, carboxymethyl cellulose solution was added, and the ultrasonic mixing process continued in two stages: the first stage used 300W of power for 30 minutes at 35°C; the second stage used 100W of power for 20 minutes at 30°C. After vacuum drying, the mixed loading material was obtained. 1.2 The mixed loading material was ultrasonically mixed with glycerin, anhydrous ethanol and purified water (ultrasonic mixing power of 550W, time of 25min, temperature of 23℃) to obtain compound pet antibacterial spray.
[0033] Comparative Example 1 In this comparative example, the formulation of the compound pet antibacterial spray was adjusted. After adjustment, the compound pet antibacterial spray includes the following raw materials by mass percentage: chlorhexidine gluconate 5%, miconazole nitrate 5%, ivermectin 5%, glycerin 8%, carboxymethyl cellulose solution 6%, mesoporous silica 14%, jojoba oil 7%, Tween 80 1%, oil-soluble azone 2%, anhydrous ethanol 12%, and purified water 35%.
[0034] The preparation method of the compound pet antibacterial spray is consistent with that in Example 2.
[0035] Comparative Example 2 In this comparative example, the formulation of the compound pet antibacterial spray was adjusted. After adjustment, the compound pet antibacterial spray includes the following raw materials by mass percentage: chlorhexidine gluconate 15%, glycerin 8%, carboxymethyl cellulose solution 6%, modified mesoporous silica 14%, jojoba oil 7%, Tween 80 1%, oil-soluble azone 2%, anhydrous ethanol 12%, and purified water 35%.
[0036] The preparation methods for modified mesoporous silica and compound pet antibacterial spray are consistent with those in Example 5.
[0037] Comparative Example 3 In this comparative example, the formulation of the compound pet antibacterial spray was adjusted. After adjustment, the compound pet antibacterial spray includes the following raw materials by mass percentage: miconazole nitrate 15%, glycerin 8%, carboxymethyl cellulose solution 6%, modified mesoporous silica 14%, jojoba oil 7%, Tween 80 1%, oil-soluble azone 2%, anhydrous ethanol 12%, and purified water 35%.
[0038] The preparation methods for modified mesoporous silica and compound pet antibacterial spray are consistent with those in Example 5.
[0039] Comparative Example 4 In this comparative example, the formulation of the compound pet antibacterial spray was adjusted. After adjustment, the compound pet antibacterial spray includes the following raw materials by mass percentage: ivermectin 15%, glycerin 8%, carboxymethyl cellulose solution 6%, modified mesoporous silica 14%, jojoba oil 7%, Tween 80 1%, oil-soluble azone 2%, anhydrous ethanol 12%, and purified water 35%.
[0040] The preparation methods for modified mesoporous silica and compound pet antibacterial spray are consistent with those in Example 5.
[0041] Blank group In this blank group, the formulation of the compound pet antibacterial spray was adjusted. After adjustment, the compound pet antibacterial spray includes the following raw materials by mass percentage: chlorhexidine gluconate 5%, miconazole nitrate 5%, ivermectin 5%, glycerin 8%, carboxymethyl cellulose solution 6%, jojoba oil 7%, Tween 80 1%, oil-soluble azone 2%, anhydrous ethanol 12%, and purified water 49%.
[0042] The preparation method of compound antibacterial spray for pets, with specific steps as follows: 1.1 Chlorhexidine gluconate, miconazole nitrate, ivermectin, jojoba oil, oil-soluble azone, and Tween 80 were ultrasonically mixed. The ultrasonic mixing process consisted of two stages: the first stage used 400W of power for 40 minutes at 23°C; the second stage used 150W of power for 30 minutes at 45°C. Then, carboxymethyl cellulose solution was added, and ultrasonic mixing was continued in two stages: the first stage used 300W of power for 30 minutes at 35°C; the second stage used 100W of power for 20 minutes at 30°C. After vacuum drying, the mixed material was obtained. 1.2 The mixture was ultrasonically mixed with glycerin, anhydrous ethanol and purified water (ultrasonic mixing power of 550W, time of 25min, temperature of 23℃) to obtain compound pet antibacterial spray.
[0043] III. Experiment (1) Antibacterial performance test of compound pet antibacterial spray under shade and strong light irradiation treatment Shade-avoiding compound antibacterial spray for pets: The compound antibacterial sprays for pets prepared in Examples 1-7, Comparative Examples 1-4 and the blank group were placed under an illuminance of 5-50 lx for 65 days.
[0044] Compound pet antibacterial spray treated with strong light: The compound pet antibacterial sprays prepared in Examples 1-7, Comparative Examples 1-4 and the blank group were placed under an illuminance of 9000-1000 lx for 65 days.
[0045] Experimental Methods: Two common pathogenic fungi in canine dermatophyte cases, Microsporum canis and Malassezia, were selected. SDA medium was prepared using standard methods. The two pathogenic fungi were inoculated into the center of the SDA medium and incubated at 28°C for 7-10 days. The bacterial concentration was then adjusted to 7.8 × 10⁻⁶. 6The compound pet antibacterial spray of this invention was diluted 3-18 times with water before use (5 times dilution was used in this experiment). It was sprayed onto the surface of SDA culture medium using a nozzle at a dosage of 0.5 g / mL. The antibacterial rate was statistically analyzed at 1 h and 6 h, as shown in Table 1. Table 1. Statistical analysis of efficacy test data for compound pet antibacterial sprays treated with shade and strong light. ; Experimental conclusions: Based on the data analysis in Table 1, it can be seen that the modified mesoporous silica prepared by sequentially modifying D-cysteine and polyethyleneimine in this invention, loaded with chlorhexidine gluconate, miconazole nitrate, ivermectin, jojoba oil, Tween 80, and oil-soluble azone in a multilayer form, can achieve a long-lasting antibacterial function. Moreover, the prepared compound pet antibacterial spray has good storage stability, and its antibacterial performance deteriorates less under strong light irradiation conditions. Among them, the group in Example 5 is the best. In addition, the combination of chlorhexidine gluconate, miconazole nitrate, and ivermectin may have a synergistic effect. When used alone, their long-lasting antibacterial performance is poor, but when the three are used in combination, the sustained antibacterial performance is significantly improved.
[0046] (2) Antibacterial performance test of compound pet antibacterial spray after high temperature and room temperature storage treatment High-temperature storage treatment of compound pet antibacterial spray: The compound pet antibacterial sprays prepared in Examples 1-7, Comparative Examples 1-4 and the blank group were kept at a high temperature of 45°C for 3 months.
[0047] Compound pet antibacterial spray for room temperature storage treatment: The compound pet antibacterial sprays prepared in Examples 1-7, Comparative Examples 1-4 and the blank group were kept at room temperature of 20°C for 3 months.
[0048] The experimental method is the same as above, and the specific experimental data are shown in Table 2: Table 2. Statistical analysis of efficacy test data for compound pet antibacterial sprays stored at room temperature and high temperature. ; Experimental conclusion: Based on the data in Table 2, it can be seen that the compound pet antibacterial spray prepared according to the method of the present invention has a high antibacterial effect on the pathogenic fungi of canine dermatophyte disease, and the effect is long-lasting. Moreover, it can still maintain relatively stable performance when stored in a high-temperature environment. This shows that the compound pet antibacterial spray of the present invention has good storage stability.
[0049] (3) Safety test One hundred and thirty healthy ICR mice, weighing 20-30g, were randomly divided into experimental groups (Examples 1-7, Comparative Examples 1-4, and blank group) and control groups, for a total of 13 groups, with 10 mice in each group, half male and half female. The experimental groups were given compound pet antibacterial spray at a concentration of 300mg / mL, administered by gavage at a dose of 0.2mL / 10g body weight three times, with an interval of 8 hours between each dose. The control group was given physiological saline instead of compound pet antibacterial spray. The mice were observed for 7 consecutive days after administration. On the 7th day, all mice were euthanized by cervical dislocation. The pathological changes of the heart, liver, spleen, lungs, kidneys and other organs were recorded by necropsy.
[0050] The experimental results showed that the mice in the experimental group did not show any abnormal changes in spirit, appetite, or activity compared to the control group, and no pathological changes were observed in their internal organs; thus, it is indicated that the compound antibacterial spray for pets of the present invention has good safety.
[0051] (4) Clinical trials of drug efficacy Experimental subjects: 168 cats and dogs infected with Microsporum canis in a pet hospital clinic, with symptoms including hair loss, itching and scaling.
[0052] Experimental procedure: Sick pets were randomly given the drug, with a total of 6 groups of 28 pets in each group.
[0053] Administration method: Dilute the compound pet antibacterial spray with water 5 times to obtain a diluted solution, then spray the diluted solution directly and evenly onto the affected area twice a day, with each dose being 0.1-5g / kg (2g / kg was used in this experiment), for 14 consecutive days. The cure rate (complete disappearance of symptoms and normal skin recovery) is shown in Table 3. Table 3 Clinical trial data on drug efficacy ; Experimental conclusion: Based on the data in Table 3, compared with the comparative group and the blank group, the compound pet antibacterial spray of Example 5 of the present invention has a higher cure rate, indicating that it has a better clinical trial effect.
[0054] The experiments described above only illustrate several embodiments of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention.
Claims
1. A compound antibacterial spray for pets, characterized in that: The compound pet antibacterial spray comprises the following raw materials in weight percentage: chlorhexidine gluconate 3-7%, miconazole nitrate 3-8%, ivermectin 3-8%, glycerin 5-10%, carboxymethyl cellulose solution 2-8%, modified mesoporous silica 12-18%, jojoba oil 5-10%, Tween 80 0.1-2%, oil-soluble azone 1-3%, anhydrous ethanol 8-15%, and purified water 35-45%. The modified mesoporous silica is obtained by modification with D-cysteine and / or polyethyleneimine.
2. The compound antibacterial spray for pets according to claim 1, characterized in that: The compound pet antibacterial spray comprises the following raw materials by weight percentage: chlorhexidine gluconate 5%, miconazole nitrate 5%, ivermectin 5%, glycerin 8%, carboxymethyl cellulose solution 6%, modified mesoporous silica 14%, jojoba oil 7%, Tween 80 1%, oil-soluble azone 2%, anhydrous ethanol 12%, and purified water 35%.
3. The compound pet antibacterial spray according to claim 2, characterized in that: The modified mesoporous silica is obtained by dispersing mesoporous silica in an 8-12 mg / mL D-cysteine solution at a certain ratio, stirring at 35-45°C for 12-18 h, then adding 0.1-1.5 g / mL polyethyleneimine solution at a certain ratio, heating in a water bath at 40-45°C for 2-3 h, stirring at 80-100 r / min during the process, and then washing with pure water, filtering and drying to obtain the modified mesoporous silica.
4. A compound antibacterial spray for pets according to claim 3, characterized in that: The mesoporous silicon has a particle size of 200-300 nm.
5. A compound antibacterial spray for pets according to claim 3, characterized in that: The mass ratio of the mesoporous silica to the 8-12 mg / mL L-cysteine solution is 1:3-5; the mass ratio of the mesoporous silica to the 0.1-1.5 g / mL polyethyleneimine solution is 1:5-10.
6. A method for preparing a compound pet antibacterial spray as described in any one of claims 1-5, characterized in that: The specific steps are as follows: (1) Chlorhexidine gluconate, miconazole nitrate, ivermectin and modified mesoporous silica were ultrasonically mixed, and then half of the total amount of carboxymethyl cellulose solution was added. Ultrasonic mixing was continued, and the load material was obtained after vacuum drying. (2) Load material one is ultrasonically mixed with jojoba oil, oil-soluble azone and Tween 80, and then the remaining amount of carboxymethyl cellulose solution is added. Ultrasonic mixing is continued, and after vacuum drying, load material two is obtained. (3) The loading material II was ultrasonically mixed with glycerol, anhydrous ethanol and purified water to obtain a compound pet antibacterial spray.
7. The preparation method of a compound pet antibacterial spray according to claim 6, characterized in that: In step one, the ultrasonic mixing treatment power is 300-400W, the time is 30-40min, and the temperature is 20-40℃; in step two, the ultrasonic mixing treatment power is 100-150W, the time is 20-30min, and the temperature is 30-45℃; in step three, the ultrasonic mixing treatment power is 500-700W, the time is 20-30min, and the temperature is 20-25℃.
8. The use of a compound pet antibacterial spray as described in any one of claims 1-5 in the preparation of a drug for treating or improving pet skin diseases.
9. The application according to claim 9, characterized in that: The topical dosage of the compound pet antibacterial spray is 1-3 times a day, with each application being 0.02-1g / kg.