Indoor high-density breeding method for trachinotus ovatus

Abstract

The invention relates to the technical field of aquaculture, in particular to an indoor high-density breeding method for trachinotus ovatus. Comprising the steps that roes are hatched in a hatching barrel, and then live fertilized roes are fished into a nursery pond; hatching in still water to form membranes; adding a complex microbial inoculant I and a fertilizer and water additive every day after film emergence; starting to feed initial feed for the fries at the third day age, and adjusting feeding according to feeding conditions; concentrated chlorella is supplemented every day, bottom sewage is discharged, and isothermal new water is supplemented; the dissolved oxygen is maintained to be 5.5 to 6 mg / L; adding photosynthetic bacteria in the morning from the fifth day, and adding a complex microbial inoculant II in the afternoon; from the age of 13 days, fry are bred under the semi-running water condition; feeding fairy shrimps at the age of 13-20 days every day; starting to add frozen krill when the krill is 21 days old; transferring into a circulating water system after the krill is 30 days old, gradually reducing the feeding amount of the frozen krill, finally replacing all the frozen krill with artificial compound feed, supplementing concentrated chlorella every day, and adding photosynthetic bacteria and a complex microbial inoculant I. The method has the advantages of improving fry survival rate and quality.

Description

Technical Field

[0001] This invention relates to the field of aquaculture technology, and in particular to a method for high-density indoor breeding of golden pomfret. Background Technology

[0002] Traditional golden pomfret breeding mainly uses open-air ponds or simple greenhouses, which rely heavily on natural water temperature and quality conditions. The breeding cycle is limited by the season and has the following technical drawbacks: (1) Poor water quality control: Open systems are difficult to effectively control the accumulation of harmful substances such as ammonia nitrogen and nitrite, which can easily lead to fish fry diseases.

[0003] (2) Extensive feed management: lack of a scientific feed transition system, fish fry suffer from malnutrition or death due to palatability issues.

[0004] (3) Low environmental stability: It is greatly affected by weather and temperature fluctuations, and the fish fry have a strong stress response, with a survival rate generally below 30%.

[0005] (4) Inefficient water quality control: Water quality is maintained by relying on high-frequency water exchange (daily water exchange rate > 50%), resulting in serious waste of water resources.

[0006] (5) The addition of microecological preparations is crude: such as a fixed dose of EM bacteria throughout the process. Insufficient algal community construction in the early stage leads to limited rotifer reproduction. When the organic load increases dramatically in the later stage, the decomposition capacity of EM bacteria is insufficient, resulting in extremely high ammonia nitrogen content.

[0007] To address the aforementioned issues, there is an urgent need to develop an artificial breeding method for golden pomfret that provides stable and controllable environmental conditions and can improve the survival rate and yield per unit area. Summary of the Invention

[0008] To solve the above problems, this invention provides a method for high-density indoor breeding of golden pomfret.

[0009] The purpose of this invention is to provide a method for high-density indoor breeding of golden pomfret, which specifically includes the following steps: S1. Fish egg hatching: Filtered and disinfected seawater is injected into the hatching tank, the water temperature is controlled at 26±1℃, the tank is kept closed and still, and oxygen is introduced; fertilized eggs are introduced, and after the fertilized eggs develop to the heartbeat stage, the hatching tank is briefly stopped from being ventilated, dead eggs at the bottom of the hatching tank are removed, and the live fertilized eggs floating on the surface are scooped from the hatching tank into the nursery pond. S2.1~2-day static water cultivation: In a seedling pond with static water conditions, the seedlings hatch and emerge from the membrane after about 3~5 hours; after hatching, add 100~150mL of compound bacterial agent I and 20~50mL of fertilizer and water additive to the seedling pond every day; compound bacterial agent I includes Pediococcus pentosaceus and Bacillus subtilis; S3.3~12-day-old still water rearing: Start feeding the fry with starter feed on the 3rd day of age, adjusting the feeding according to the fry's feeding behavior; supplement with concentrated Chlorella daily, drain the bottom wastewater and add isothermal fresh water, with a water exchange rate of 5~10%; maintain dissolved oxygen at 5.5~6mg / L, and maintain a daily light intensity of ≥3000Lux and a light duration of ≥12h for the entire pond; from the 5th day onwards, add 70~150mL of photosynthetic bacteria in the morning and 100~150mL of compound bacterial agent II in the afternoon; compound bacterial agent II contains Bacillus and lactic acid bacteria; S4.13-30 Days Old Semi-Flowing Water Culture: Starting at 13 days old, fry are cultured under semi-flowing water conditions; daily supplementation with concentrated Chlorella, 50-100 mL of photosynthetic bacteria and 450-550 mL of compound bacterial agent I, and maintaining a daily water exchange rate of 10-20%; from 13 to 20 days old, feed brine shrimp every morning, and supplement with frozen fleas or brine shrimp in the afternoon depending on the condition of the fry; from 21 to 30 days old, start adding frozen krill, and gradually reduce the amount of brine shrimp and frozen fleas fed. S5. Recirculating Aquaculture after 30 Days: After the fry reach 30 days of age, transfer them to the biological filter of the recirculating aquaculture system, with dissolved oxygen at 7-8 mg / L and water temperature at 28±0.5℃. Supplement daily with 100-200 mL of concentrated Chlorella, 450-550 mL of compound bacterial agent I, and 50-100 mL of photosynthetic bacteria. Simultaneously feed frozen krill and formulated feed, gradually reducing the amount of frozen krill until it is completely replaced by formulated feed. Feed 4-8 times a day, with each feeding amount determined by the fry finishing the food within 20 minutes. Regularly remove uneaten feed.

[0010] Preferably, the fertilizer additive contains betaine hydrochloride and compound vitamins; the concentrated Chlorella cell concentration is 1×10⁻⁶. 7 The effective viable count of the photosynthetic bacteria is ≥1×10⁻⁶ / mL; 8 CFU / mL; the particle size of the artificial compound feed is 0.3~0.5mm, and the protein content is greater than 50%.

[0011] Preferably, the incubation tank is a 500L polyethylene incubation tank; The seedling pond is 30m² 3 The PP board seedling tank is equipped with an ultrafiltration seawater system, an air stone oxygen supply device, and a water temperature control system; the circulating water system includes a microfilter, a biochemical filter filled with polyurethane biological balls, and an ultraviolet sterilization device, with a treatment capacity of 80~120m³. 3 / h; Before raising seedlings, the seedling ponds are disinfected with potassium permanganate.

[0012] Preferably, in step S1, 100g of fertilized eggs are placed in each incubation tank; In step S2, 80-120g of live fertilized eggs are placed in each nursery pond; during the cultivation period, the oxygen level is adjusted until small bubbles appear on the water surface, and the opening of the fish fry's eyes and mouths is checked every 12 hours.

[0013] Preferably, in step S3, the initial feed for 3-7 day old fry is SS-type rotifers, with a density of 50-100 SS-type rotifers / mL in the water. The feeding behavior of the fry is observed, and the amount of SS-type rotifers is reduced or increased based on whether the fry are full. Starting from 7 days old, fortified brine shrimp are gradually added, and the amount of SS-type rotifers is gradually reduced. The fortified brine shrimp are prepared by hatching brine shrimp and then culturing them in a hatching tank with added yeast. Continue to concentrate the Chlorella until the water turns emerald green and the bottom of the pond is no longer visible; the bottom wastewater is discharged through the bottom valve of the seedling pond.

[0014] Preferably, the method for adjusting the semi-flowing water in step S4 is as follows: rotate the inlet of the seedling pond to a 45-degree angle with the water surface to start the flow of water, and drive the water in the seedling pond to rotate. The water flow rate is controlled at 0.05~0.15m / s; the dissolved oxygen is controlled at 6~7mg / L and the water temperature is controlled at 27±1℃.

[0015] Preferably, step S4 further includes: starting from 15 days old, reducing the amount of Artemia feeding and increasing the amount of frozen flea feeding to 70% of the total feeding, with overlapping feeding for 7 days.

[0016] Preferably, the brine shrimp need to be deshelled 24 hours after hatching before feeding; the frozen fleas are rinsed in clean seawater at 28-32℃ until the water is clear before feeding; the frozen krill have a protein content of more than 60% and are chopped to 1mm. 3 Feed afterward.

[0017] Preferably, step S5 further includes: before the fish fry are transferred into the recirculating water system, cleaning the biological filter, injecting new water and aerating for 24 hours; placing nano discs around the biological filter to aerate and ensure sufficient oxygen. After completely replacing the feed with artificial compound feed, feed the animals 4 times a day.

[0018] Preferably, the pH value is maintained at 8 to 8.2 in step S3; and the water circulation cycle is maintained at 1 to 2 hours in step S5.

[0019] Compared with the prior art, the present invention can achieve the following beneficial effects: This invention provides a method for high-density indoor breeding of golden pomfret fry. Based on the characteristics of different developmental stages of golden pomfret fry, a phased dynamic breeding system, a precise feed transition system, and a water microecological synergistic regulation system are specifically designed, as follows: Phased Dynamic Breeding System: Based on the physiological characteristics of the fry's age (1-12 days, 13-30 days, and after 30 days), a "closed still water - semi-flowing water - recirculating water" mode is adopted sequentially to match the environmental needs of different growth stages. A precise feed transition system has been developed. Based on the fry's mouthpart development (mouth diameter increases from 0.1mm to 1.2mm) and digestive capacity, a four-stage progressive feeding system of "SS rotifers - Artemia - frozen fleas / frozen krill - formulated feed" is designed, with overlapping feeding at each stage to reduce palatability barriers. A water microecological synergistic regulation system is established: Chlorella (initially 1-20 days old), photosynthetic bacteria, probiotics, and potent EM are supplemented in stages to construct an "algae-bacteria-insect" symbiotic system, enhancing the water's self-purification capacity. This method significantly improves the survival rate of fish fry, with an overall survival rate of over 60%, which is 2 to 3 times higher than the traditional method. The mortality rate during the feed transition period is significantly reduced. Off-season fish fry production can be carried out to supply the market in advance. The water quality is stable and controllable, with ammonia nitrogen and nitrite concentrations below the standard values. The fry are of excellent quality, free from bacteria, viruses, parasites, etc., and have high uniformity in body length and size. Attached Figure Description

[0020] Figure 1 This is a flowchart of a method for high-density indoor breeding of golden pomfret provided according to an embodiment of the present invention. Detailed Implementation

[0021] In the following description, embodiments of the invention will be described with reference to the accompanying drawings. In the description below, the same modules are denoted by the same reference numerals. Where the same reference numerals are used, their names and functions are also the same. Therefore, their detailed description will not be repeated.

[0022] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not constitute a limitation thereof.

[0023] This invention provides a method for high-density indoor breeding of golden pomfret, specifically including the following steps: S1. Fish egg hatching: Filtered and disinfected seawater is injected into the hatching tank, the water temperature is controlled at 26±1℃, the tank is kept closed and still, and oxygen is introduced; fertilized eggs are introduced, and after the fertilized eggs develop to the heartbeat stage, the hatching tank is briefly stopped from being ventilated, dead eggs at the bottom of the hatching tank are removed, and the live fertilized eggs floating on the surface are scooped from the hatching tank into the nursery pond. Specifically, the incubation tank is a 500L polyethylene incubation tank; the seedling pond is 30m².3 The circular PP board nursery is equipped with an ultrafiltration seawater system (flux 3000L / h), an air stone oxygen supply device, and a water temperature control system (accuracy ±0.5℃); the filtration is ultrafiltration with an accuracy of 0.05μm, and the disinfection is ultraviolet disinfection; 100g of fertilized eggs are placed in each hatching tank.

[0024] S2.1~2-day-old still water rearing: In a still water rearing pond, the fry hatch and emerge after about 3~5 hours; after hatching, add 100~150mL of compound bacterial agent I and 20~50mL of fertilizer additive to the rearing pond every day; during this period, adjust the oxygen level until small bubbles appear on the water surface, and check the opening of the eyes and mouth of the fry every 12 hours. Specifically, about 100g of live fertilized eggs are placed in each seedling pond; compound microbial agent I and fertilizer additive are added every morning; compound microbial agent I includes Pediococcus pentosaceus, Bacillus, etc., and fertilizer additive contains betaine hydrochloride and compound vitamins.

[0025] S3.3~12-day-old still water rearing: After the fry start feeding, feed them starter food, adjusting the feeding according to their feeding behavior; supplement with concentrated Chlorella daily until the water color is emerald green and the bottom of the pond is no longer visible; drain the bottom wastewater daily and add isothermal fresh water, with a water exchange rate of 5~10%; maintain dissolved oxygen at 5.5~6 mg / L, and maintain a daily light intensity of ≥3000 Lux and a light duration of ≥12h for the entire pond; from the 5th day, add 70~150mL of photosynthetic bacteria in the morning and 100~150mL of compound bacterial agent II in the afternoon; from the 7th day, measure water quality indicators daily, and when the concentration of ammonia nitrogen or nitrite is too high, increase the addition of concentrated Chlorella and photosynthetic bacteria, while increasing the water exchange rate; Specifically, the initial feed for 3-7 day old fry is SS-type rotifers, with a density of 50-100 SS-type rotifers / mL in the water. Observe the feeding behavior of the fry and adjust the amount of SS-type rotifers feeding based on whether they are full. Starting from 7 days old, gradually add fortified brine shrimp and gradually reduce the amount of SS-type rotifers feeding. The fortified brine shrimp is prepared by hatching brine shrimp and then culturing them in a hatching tank with added yeast. Supplement daily with 750-1500 mL of concentrated Chlorella, with a cell concentration of 1×10⁻⁶. 7 CFU / mL; Effective viable count of photosynthetic bacteria ≥ 1 × 10⁻⁶ 8 CFU / mL; Compound bacterial agent II contains Bacillus and lactic acid bacteria; Bottom wastewater is discharged through the bottom valve of the seedling pond; Lighting follows the sunrise and sunset pattern, gradually increasing or decreasing the light intensity.

[0026] S4.13-30 Days Old Semi-Flowing Water Culture: Starting at 13 days old, fry are cultured under semi-flowing water conditions; daily supplementation with concentrated Chlorella, 50-100 mL of photosynthetic bacteria and 450-550 mL of compound bacterial agent I, and maintaining a daily water exchange rate of 10-20%; from 13 to 20 days old, feed brine shrimp every morning, and supplement with frozen fleas or brine shrimp in the afternoon depending on the condition of the fry; from 21 to 30 days old, start adding frozen krill, and gradually reduce the amount of brine shrimp and frozen fleas fed. Specifically, the adjustment method for semi-flowing water is as follows: Rotate the inlet of the seedling pond to a 45-degree angle with the water surface to start the flow of water, and rotate the water in the seedling pond. Control the water flow rate at 0.05~0.15m / s; control the dissolved oxygen at 6~7mg / L and the water temperature at 27±1℃. Supplement daily with 200-500 mL of concentrated Chlorella, with a cell concentration of 1×10⁻⁶. 7 CFU / mL; Effective viable count of photosynthetic bacteria ≥ 1 × 10⁻⁶ 8 CFU / mL, compound bacterial agent I includes Pediococcus pentosacchari, Bacillus, etc., and photosynthetic bacteria and compound bacterial agent I are used to decompose residual organic matter; Starting from 15 days old, reduce the amount of Artemia feeding and increase the amount of frozen flea feeding to 70% of the total feeding, overlapping the feeding for 7 days; Artemia larvae need to be shelled 24 hours after hatching; frozen fleas should be stored at -20℃, and after thawing, rinsed with clean seawater at 28~32℃ until the water is clear before feeding; frozen krill with a protein content greater than 60% should be chopped to 1mm. 3 Feed afterward.

[0027] S5. Recirculating Aquaculture after 30 Days: After the fry reach 30 days of age, transfer them to the biological filter of the recirculating aquaculture system, maintaining a water circulation cycle of 1-2 hours, dissolved oxygen of 7-8 mg / L, and water temperature of 28±0.5℃. Supplement daily with 100-200 mL of concentrated Chlorella, 450-550 mL of compound bacterial agent I, and 50-100 mL of photosynthetic bacteria. Simultaneously feed frozen krill and formulated feed, gradually reducing the amount of frozen krill until it is completely replaced by formulated feed. Feed 4-8 times a day, with each feeding amount determined by the amount the fry can finish consuming within 20 minutes. Begin cleaning up uneaten feed 25-40 minutes after feeding to prevent it from settling and rotting at the bottom. Specifically, the circulating water system (treatment capacity 100m³) 3 / h) includes a microfiltration unit (filtration accuracy 50μm) and a biological filter (volume 400m³). 3 Filled with polyurethane biospheres, with a nitrifying bacteria loading of ≥500g / m³ 3 ), UV sterilizer (power 2000W, wavelength 254nm, irradiation intensity 30mJ / cm²) 2Water flows obliquely into the pool through the outlet, causing water flow. The entire water circulation cycle is ≤2 hours. Various water quality indicators are checked regularly to ensure the normal operation of the system. The artificial feed is Seahorse brand No. 03 artificial feed with a particle size of 0.3~0.5mm and a protein content of 52%. After replacing all feeds with artificial feed, feed 4 times a day. Before transferring the fish fry into the recirculating aquaculture system, clean the biological filter, add fresh water and aerate for 24 hours; place nano discs around the biological filter to aerate and ensure sufficient oxygen.

[0028] Example 1 This embodiment provides a method for high-density indoor breeding of golden pomfret, which specifically includes the following steps: S1. Egg Hatching: Seawater that has undergone 0.05μm ultrafiltration and UV disinfection is injected into a 500L polyethylene hatching tank. The water temperature is controlled at 26±1℃, maintaining a closed, still environment with oxygenation. 100g of fertilized eggs are placed in each hatching tank. After the fertilized eggs develop to the heartbeat stage, the tank is briefly aerated. Dead eggs at the bottom of the tank are removed, and the live fertilized eggs floating on the surface are then transferred from the hatching tank to the nursery pond. 25m³ of ultrafiltered seawater is added to the nursery pond beforehand. 3 Adjust the water temperature to 26.5℃.

[0029] S2.1~2-day-old still water rearing: In a still water rearing pond, the fry hatch and emerge from the membrane after about 3~5 hours; after hatching, add 120mL of compound bacterial agent I and 25mL of fertilizer additive to the rearing pond every morning; during this period, adjust the oxygen level until small bubbles appear on the water surface, and check the opening of the eyes and mouth of the fry every 12 hours; compound bacterial agent I includes Pediococcus pentosaccharide, Bacillus, etc., and fertilizer additive contains betaine hydrochloride and compound vitamins; The seedling bed is 30m 3 The circular PP board seedling tank is equipped with an ultrafiltration seawater system (flux 3000L / h), an air stone oxygen supply device, and a water temperature control system (accuracy ±0.5℃).

[0030] S3.3~12-day-old still water rearing: After the fry begin to eat, add SS-type rotifers as their first feed, maintaining a density of 100 SS-type rotifers / mL, twice daily. Samples should be taken 2 hours after each feeding to observe uneaten feed. Oxygen should be supplied through an air stone aeration device (pressure adjusted to 0.05 MPa) to maintain dissolved oxygen at 5.5~6 mg / L. Add 1000 mL of concentrated Chlorella (cell concentration 1×10⁻⁶) every morning. 7 In the afternoon, observe the algae color. If it becomes lighter, add more Chlorella until the water color is emerald green, just enough to obscure the bottom of the pond. From the 5th day onwards, add 100mL of photosynthetic bacteria (effective viable count ≥1×10⁻⁶) every morning. 8In the afternoon, add 120mL of compound bacterial agent II (containing Bacillus and lactic acid bacteria); drain the bottom sewage daily through the bottom valve, with a drainage volume of 10%; replenish with isothermal fresh water; maintain a daily light intensity of ≥3000Lux and a light duration of ≥12h for the entire pond, gradually increasing or decreasing the light intensity according to the sunrise and sunset pattern; from the 7th day, measure water quality indicators daily, and when the concentration of ammonia nitrogen or nitrite is too high, add concentrated Chlorella and photosynthetic bacteria, while increasing the water exchange volume; maintain the pH value at 8~8.2. During this stage, by stabilizing the environment to reduce fish fry stress, the survival rate of the first feeding increases to over 90%. The survival rate of 12-day-old fish fry is 65%, with an average body length of 0.6cm and a deformity rate of <5‰.

[0031] In this step, the method for observing uneaten feed is as follows: Two hours after feeding, take a 200mL sample from the edge of the pond using a beaker and observe the amount of rotifers remaining under directional light. If there are no rotifers on the beaker wall, add another 500mL of rotifer solution; if 10-20 rotifers remain per field of view, it indicates satiation. This method can achieve a satiation rate of 95% for fish fry, avoiding fry mortality due to insufficient feed, while also allowing rotifers to reproduce and appropriately supplement the initial feed.

[0032] During this period, starting from 7 days old, Artemia larvae were fed with supplemented nutrition (after hatching, yeast was added to the hatching tank to supplement nutrition), and the amount of rotifers fed was gradually reduced. Artemia larvae were hatched once in the morning and once in the afternoon each day (hatching rate ≥85%), with 500g of Artemia larvae eggs hatched per tank each time. The eggshells were filtered through a 40-mesh sieve before feeding.

[0033] S4.13~30 Days Old Semi-Flowing Water Rearing: After 13 days of age, the fish fry's swimming ability improves, requiring appropriate flowing water to promote growth and development. Rotate the inlet of the rearing pond to a 45-degree angle with the water surface to create flowing water, rotating the water in the rearing pond. Control the water flow rate at 0.15 m / s, with a daily water exchange rate of 20%; maintain dissolved oxygen at 6~7 mg / L and water temperature at 27±1℃. Supplement daily with 200~500 mL of concentrated Chlorella (cell concentration 1×10⁻⁶). 7 Simultaneously, 100 mL of photosynthetic bacteria (effective viable count ≥ 1 × 10⁻⁶) is added daily. 8 CFU / mL), a potent compound bacterial agent I 500mL (containing Pediococcus pentosaceus, Bacillus, and other compound bacteria) to decompose residual feed and organic matter; monitor water quality indicators daily. Transition the feed to brine shrimp (shelled 24 hours after hatching) and frozen fleas (thawed and rinsed 3 times with clean seawater). From 13-20 days old, feed brine shrimp at 8:00 AM daily until satiated, and observe the fry's condition at 2:00 PM. If still satiated, feed frozen fleas; if not, supplement with brine shrimp. From 15 days old, reduce the amount of brine shrimp and increase the amount of frozen fleas to 70% of the total feed, overlapping for 7 days. From 21-30 days old, add frozen krill (chopped to 1mm). 3Gradually reduce the amount of brine shrimp and frozen fleas fed, and overlap feeding with frozen fleas for 7 days, gradually increasing the protein content of the feed (from 40% protein content of rotifers to 65% protein content of frozen krill). 24-day-old fish fry fed frozen fleas achieved a satiation rate of over 95%, and their body length increased by an average of 0.1 cm per day.

[0034] S5. Recirculating Aquaculture After 30 Days: After the fry reach 30 days of age, transfer them to the biological filter of the recirculating aquaculture system, maintaining a water circulation cycle of 1.5 hours, dissolved oxygen of 7-8 mg / L, and water temperature of 28±0.5℃. Supplement daily with 100-200 mL of concentrated Chlorella, 450-550 mL of compound bacterial agent I, and 50-100 mL of photosynthetic bacteria. Simultaneously feed frozen krill and formulated feed. When feeding, scatter both frozen krill and formulated feed into the pond, gradually reducing the number of insects and increasing the feed ratio. Gradually replace the krill with Seahorse Brand No. 03 formulated feed (particle size 0.3-0.5 mm, protein content 52%), using a "small amount, multiple times" feeding method. Each feeding should be enough for the fry to finish eating within 20 minutes. Clean up any uneaten feed 30 minutes after feeding to prevent it from settling and rotting at the bottom. The average total length of the 45-day-old fry was 3.8cm, with a survival rate of 78%, a water exchange rate of 8%, and ammonia nitrogen levels consistently below 0.03mg / L.

[0035] In this step, before the fish fry are transferred to the circulating water system, the biological filter is cleaned, new water is added and aerated for 24 hours; nano discs are placed around the biological filter to aerate the water and ensure sufficient oxygen; the fish fry are fed with Seahorse Brand No. 03 formulated feed to acclimate them, and they are fed 4 times a day (7:00, 11:00, 15:00, 18:00). After feeding, the uneaten feed is cleaned up. Daily testing of ammonia nitrogen (<0.05mg / L) and nitrite (<0.01mg / L) is conducted. 500mL of compound bacterial agent (containing Pediococcus pentosaceus, Bacillus spp., and other compound bacterial groups) is added every 2 days. The microfilter and filter screen are cleaned weekly, and the activity of nitrifying bacteria in the biochemical filter is tested monthly (ammonia nitrogen removal rate should be >95%).

[0036] The circulating water system maintains stable water quality without fluctuations, supporting high-density aquaculture with fish fry densities reaching 20,000 to 30,000 fish per cubic meter. 3 .

[0037] The water quality control of this invention is divided into three stages, as detailed below: (1) Initial water quality control: Before raising seedlings, disinfect the seedling pond with 200 ppm potassium permanganate for 4 hours. After disinfection, rinse until there is no purple residue in the pond. Inject seawater that has been ultrafiltered (0.05 μm precision) and disinfected with ultraviolet light, and disinfect with chlorine dioxide for 12 hours. After detoxification, test ammonia nitrogen <0.05 mg / L, nitrite <0.01 mg / L, and residual chlorine <0.01 mg / L. The fresh water added after draining the bottom each day needs to be aerated for 24 hours. The temperature difference between the water and the pond should be ≤0.5℃ to prevent stress on the fry.

[0038] (2) Mid-term water management: As the fish fry grow and the amount of feed increases, the water quality in the pond begins to deteriorate. When it is observed that the water in a certain pond is turbid and foam increases (signs of increased protein and ammonia nitrogen), immediately close the inlet of other ponds with good water quality and prioritize the introduction of fresh water into the pond with deteriorated water quality (increase the inlet flow rate to 200L / h) to quickly replace 1 / 3 of the water volume in the pond. At the same time, add 300-400mL of compound probiotic preparation (the main components are psychrophilic Bacillus, acid-producing Bacillus, lactic acid bacteria, and yeast) + 500mL of compound bacterial agent I (including Pediococcus pentosaceus, Bacillus, etc.) to the pond to restore the water transparency to above 30cm within 24 hours.

[0039] (3) Post-circulation water maintenance: After entering the circulation water system, monitor the water level in the storage tank daily (maintain a constant 1.5m), the foam height in the protein skimmer (control the height to 1 / 3 of the skimmer height), and the water level in the biological filter (5cm above the filter media layer). Control the water level in the storage tank by adjusting the inflow rate. If the water level in the biological filter drops, immediately check the water pump flow rate (it should be maintained at 5m). 3 ( / h) to prevent nitrifying bacteria from dying due to lack of water. Clean the microfilter screen every 2 days to ensure filtration efficiency.

[0040] The oxygen supply strategy of the present invention is as follows: (1) Early stage of fry rearing (1-12 days old): Use air stones to aerate the water at both the edge and center of the pond, with a spacing of 1.5m between the air stones. Adjust the air pressure to 0.03-0.05MPa and the bubble diameter to ≤2mm to avoid strong water flow impacting the fry. When the fry are observed to gather at the edge of the pond (signs of oxygen deficiency), gradually increase the air pressure to 0.08MPa and at the same time check the dissolved oxygen (it should quickly rise to above 5mg / L). After starting to feed rotifers, clean the air stones daily to prevent rotifers from attaching and clogging them.

[0041] (2) Mid-to-late stage of fry rearing (after 13 days of age): After the fry reach 13 days of age, use nano-aeration discs with bubble diameter <0.5mm and maintain dissolved oxygen at 6-8mg / L; 30 minutes before feeding high-protein feed, increase the oxygen supply by 20% (adjust the air pressure to 0.1MPa) to prevent a sudden drop in dissolved oxygen after feeding. If the fry show slow feeding, temporarily reduce the air pressure to 0.06MPa to reduce water disturbance, and gradually adjust it back after feeding resumes.

[0042] This invention employs multi-stage dissolved oxygen synergistic management, and the equipment is 30m². 3 The circular PP board seedling trays are equipped with an online dissolved oxygen monitoring system (accuracy ±0.1 mg / L) and an adjustable air pressure oxygen supply system. The operating steps are as follows: 1-12 days old: Hang air stones around and in the pool, with a spacing of >1m and a depth of >1.5m. Adjust the air pressure to 0.05MPa, so that the oxygen bubbles are dense and maintain the dissolved oxygen concentration at 5-6mg / L. Clean the air stones daily. 13-30 days old: Adjust the air pressure to 0.08 MPa, with obvious oxygen bubbles, and maintain the dissolved oxygen concentration at 6-7 mg / L. Increase the pressure to 0.1 MPa 30 minutes before feeding. After 30 days: Use nano aeration discs installed around the bottom of the pool to cover the entire pool with oxygen. Adjust the air pressure to 0.12MPa and maintain the dissolved oxygen concentration at 7~8mg / L. Monitor dissolved oxygen fluctuations in real time (≤±0.5mg / L) using a dissolved oxygen meter.

[0043] Technical parameters: Dissolved oxygen levels reached 100% at all stages, and the fish fry showed no signs of oxygen deficiency or stress.

[0044] Results: Fish fry feeding activity increased by 40%, and growth rate increased by 25% compared to the group with unstable dissolved oxygen.

[0045] The key technical points of this invention include the following aspects: 1. Phased dynamic aquaculture: The seedling breeding cycle is scientifically divided into three stages: "closed still water - semi-flowing water - recirculating water". Environmental parameters (such as water flow rate from 0 to 0.2 m / s and dissolved oxygen from 5 mg / L to 8 mg / L) are matched according to the physiological development law of fish fry. This breaks through the limitations of the traditional single aquaculture model and is an innovative technical path in the field of marine fish seedling breeding.

[0046] 2. Progressive Overlapping Feeding Method: The principle of "dual matching of feed particle size and nutrition" is proposed. The feed particle size is progressively increased from SS rotifers (50μm) to Artemia (200μm), then to frozen fleas (500μm), and finally to formulated feed (300μm). This method is implemented in four stages, with each stage combined with overlapping feeding for transition. This solves the problem of palatability during the feed transition period of marine fish fry. This method has not been reported in similar studies.

[0047] 3. Synergistic regulation technology of circulating water and micro-ecology: Construct a three-level water quality regulation system of "physical filtration (microfiltration machine) + biological treatment (biochemical filter) + microbial enhancement (EM bacteria)" to achieve precise control of ammonia nitrogen <0.05mg / L under high-density aquaculture (20,000 to 30,000 fish / m3), which improves the treatment efficiency by 30% compared with traditional circulating water system and is a technological integration innovation.

[0048] 4. Precise control strategy for multi-dimensional environmental parameters: Establish a dynamic control model for water temperature (26~28℃), dissolved oxygen (5~8mg / L), and water flow velocity (0~0.2m / s) based on age. Through a closed-loop system of "monitoring-feedback-adjustment", the fluctuation of environmental parameters is controlled within ±5%, which significantly reduces the stress response of fish fry. This precise control method is innovative.

[0049] It should be understood that the various forms of processes shown above can be used to reorder, add, or delete steps. For example, the steps described in this invention disclosure can be executed in parallel, sequentially, or in different orders, as long as the desired result of the technical solution disclosed in this invention can be achieved, and this is not limited herein.

[0050] The specific embodiments described above do not constitute a limitation on the scope of protection of this invention. Those skilled in the art should understand that various modifications, combinations, sub-combinations, and substitutions can be made according to design requirements and other factors. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. A method for indoor high-density rearing of golden pompano, characterized in that: Specifically comprising the following steps: S1. Fish egg hatching: inject filtered and disinfected seawater into the hatching barrel, control the water temperature at 26±1℃, keep closed still water and oxygen input; put the fertilized eggs, after the fertilized eggs develop to the heart beating period, temporarily stop the oxygen input of the hatching barrel, remove the dead eggs at the bottom of the hatching barrel, and then fish the live fertilized eggs floating on the surface of the hatching barrel to the fish breeding pond; S2. 1~2 day-old still water cultivation: in the still water condition of the fish breeding pond, the membrane is hatched out after about 3~5 hours; 100~150mL of compound microbial agent I and 20~50mL of fertilizer additive are added to the fish breeding pond every day after the membrane is hatched out; the compound microbial agent I includes Pediococcus pentosaceus and Bacillus; S3. 3~12 day-old still water cultivation: the fish fry starts to feed on the opening bait at the age of 3 days, and the feeding is adjusted according to the feeding condition of the fish fry; concentrated chlorella is supplemented every day, and the bottom sewage is discharged and replaced with isothermal new water, and the water replacement amount is 5~10%; the dissolved oxygen is maintained at 5.5~6mg / L, the whole pond is maintained at a daily light intensity of ≥3000Lux and a light duration of ≥12h; from the 5th day, 70~150mL of photosynthetic bacteria are added in the morning and 100~150mL of compound microbial agent II are added in the afternoon; the compound microbial agent II contains Bacillus and lactic acid bacteria; S4. 13~30 day-old semi-flow water cultivation: the fish fry is cultivated under semi-flow water condition at the age of 13 days; concentrated chlorella is supplemented every day, 50~100mL of photosynthetic bacteria and 450~550mL of compound microbial agent I are added, and the daily water replacement amount is maintained at 10~20%; rotifer bait is fed in the morning and frozen Artemia or rotifer is determined to be supplemented according to the state of the fish fry in the afternoon at the age of 13~20 days; frozen euphausiids are added at the age of 21~30 days, and the feeding amount of rotifer and frozen Artemia is gradually reduced; S5. 30 day-old post-circulating water cultivation: after the fish fry is 30 days old, it is transferred to the biochemical filter tank of the circulating water system, the dissolved oxygen is 7~8mg / L, and the water temperature is 28±0.5℃; 100~200mL of concentrated chlorella, 450~550mL of compound microbial agent I and 50~100mL of photosynthetic bacteria are supplemented every day; frozen euphausiids and artificial compound feed are fed at the same time, and the feeding amount of frozen euphausiids is gradually reduced and finally replaced by artificial compound feed; the fish fry is fed 4~8 times a day, and the feeding amount is determined according to the feeding of the fish fry within 20 minutes; the residual feed is cleaned regularly.

2. A method for indoor high-density rearing of golden pompano as claimed in claim 1, wherein: The fertilizer and water additive contains betaine hydrochloride and compound vitamins; the cell concentration of concentrated chlorella is 1×10 7 The effective viable count of the photosynthetic bacteria is ≥1×10 8 CFU / mL; the particle size of the artificial compound feed is 0.3-0.5 mm, and the protein content is greater than 50%.

3. A method for high density indoor rearing of golden pompano as claimed in claim 1, wherein: The hatching barrel is a 500L polyethylene hatching barrel; The said rearing pond is 30m 3 The PP plate rearing pond is equipped with an ultrafiltration seawater system, a gas stone oxygen supply device, and a water temperature control system; the circulating water system comprises a microfilter, a biochemical filter filled with polyurethane biological balls, and an ultraviolet sterilization device, and the treatment capacity is 80-120m 3 Before rearing, the rearing pond is disinfected by using potassium permanganate.

4. A method for high density indoor rearing of golden pompano as claimed in claim 1, wherein: In the step S1, 100g of fertilized eggs are put into each hatching barrel; In the step S2, 80~120g of live fertilized eggs are put into each fish breeding pond; the oxygen size is adjusted to small bubbles on the water surface during cultivation, and the eye opening and mouth opening of the fish fry are checked every 12h.

5. A method for high density indoor rearing of golden pompano as claimed in claim 1, wherein: In the step S3, the opening bait at the age of 3~7 days is SS rotifer, and the density of SS rotifer in the water body is 50~100 pieces / mL; the feeding amount of SS rotifer is reduced or increased according to the feeding condition of the fish fry; the SS rotifer is gradually added with enhanced nutrition from the age of 7 days, and the feeding amount of SS rotifer is gradually reduced; the SS rotifer with enhanced nutrition is obtained by culturing the rotifer in a hatching barrel with added yeast after hatching; The concentrated chlorella is added to the water until the water is emerald green and the bottom of the tank is not visible; the bottom layer of sewage is discharged through the tank bottom valve of the rearing tank.

6. A method for high density indoor rearing of golden pompano as claimed in claim 1, wherein: The half-flow adjustment method in the step S4 is as follows: the water inlet of the rearing tank is rotated at 45 degrees to the water surface, the flow water is developed, the rearing tank is pushed to rotate, the water flow rate is controlled to be 0.05-0.15 m / s, the dissolved oxygen is controlled to be 6-7 mg / L, and the water temperature is controlled to be 27±1 ℃.

7. A method for high density indoor rearing of golden pompano as claimed in claim 1, wherein: The step S4 further comprises: from 15 days old, the amount of feeding of the brine shrimp is reduced, the amount of feeding of the frozen fleas is increased to 70% of the total amount of feeding, and the feeding is overlapped for 7 days.

8. A method for high density indoor rearing of golden pompano as claimed in claim 1, wherein: The brine shrimp needs to be fed after being de-shelled 24 hours after hatching; the frozen fleas are fed after being rinsed with clean seawater at 28-32°C until the water is clear; the frozen phosphorus shrimps have a protein content of more than 60% and are cut into 1mm 3 pieces before being fed.

9. A method for high density indoor rearing of golden pompano as claimed in claim 1, wherein: The step S5 further comprises: before the fry is transferred into the circulating water system, the biochemical filter tank is cleaned, new water is injected, and aeration is performed for 24 hours; the nano disc is placed, oxygen is exposed around the biochemical filter tank, and sufficient oxygen is ensured. After all the artificial compound feed is replaced, the fry is fed 4 times a day.

10. A method for high density indoor rearing of golden pompano as claimed in claim 1, wherein: In the step S3, the pH value is maintained to be 8-8.2; and in the step S5, the water body circulation period is maintained to be 1-2 hours.

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