Soil conditioner containing earthworm enzymatic hydrolysate and preparation method thereof

Abstract

The invention relates to the technical field of soil amendments, in particular to a soil amendment containing earthworm enzymatic hydrolysate and a preparation method of the soil amendment. The soil conditioner containing the earthworm enzymatic hydrolysate comprises the following components: earthworm enzymatic hydrolysate microcapsules and adjuvant particles in a mass ratio of (2-4): 1, the earthworm enzymatic hydrolysate is prepared by performing enzymolysis on fresh earthworm homogenate by compound enzyme, and the compound enzyme comprises cellulase and protease; the wall material of the earthworm enzymatic hydrolysate microcapsule comprises at least one of chitosan, calcium alginate, gelatin and Arabic gum; the auxiliary agent comprises humic acid and inorganic natural minerals. According to the soil conditioner containing the earthworm enzymatic hydrolysate, the earthworm enzymatic hydrolysate is wrapped by the microcapsules, the retention rate of active ingredients in the earthworm enzymatic hydrolysate can be remarkably increased, and the soil condition can be further improved by matching with humic acid and inorganic natural minerals for use.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of soil improver, in particular to a soil improver containing earthworm enzymatic hydrolysate and a preparation method thereof. BACKGROUND

[0002] Soil degradation mainly manifests as soil acidification, compaction, and decline in fertility. In order to improve the soil condition, various soil improvers have emerged.

[0003] In the prior art, traditional chemical improvers such as lime and chemical fertilizer can alleviate soil degradation to some extent, but can easily lead to secondary pollution of the soil and have a short-term improvement effect, and thus cannot fundamentally solve the problem of soil degradation. Biological improvers such as straw humic substances and microbial preparations are relatively environmentally friendly, but have defects such as instability of active ingredients and short action period, and thus cannot meet the long-term needs of agricultural production.

[0004] Earthworms are rich in biological active substances such as proteins, active enzymes, polypeptides, and amino acids, and the enzymatic hydrolysate of earthworms has the potential to promote the formation of soil aggregate structure, activate nutrients, and improve biological activity. Therefore, soil improvers based on earthworm enzymatic hydrolysate have become a research hotspot. However, existing products are mostly directly packaged earthworm enzymatic hydrolysate, which is easily affected by factors such as microbial contamination and oxidation during storage and transportation, and thus is prone to decomposition and deterioration, resulting in a decrease in the content of effective components and a reduction in the efficacy of the improver. Meanwhile, when directly applied, the earthworm enzymatic hydrolysate is easily rapidly decomposed by soil microorganisms, resulting in a short action period and an inability to continuously exert an improvement effect.

[0005] In addition, the existing microencapsulation technology often uses chemical crosslinking agents to embed the enzymatic hydrolysate, which may introduce harmful substances and does not meet the development needs of green agriculture, thereby limiting its wide application in the agricultural field. It can be seen that the existing technology needs to be improved and enhanced. SUMMARY

[0006] In view of the above deficiencies of the prior art, the present application aims to provide a soil improver containing earthworm enzymatic hydrolysate and a preparation method thereof, and aims to solve the technical problems of a short action period of earthworm enzymatic hydrolysate and a short-term effect of existing soil improvers.

[0007] In order to achieve the above-mentioned purpose, the present application adopts the following technical solutions: The present application provides a soil improver containing earthworm enzymatic hydrolysate, which comprises the following components: earthworm enzymatic hydrolysate microcapsules and auxiliary agent particles in a mass ratio of (2-4):1; the earthworm enzymatic hydrolysate is prepared by enzymatic hydrolysis of fresh earthworm homogenate using a complex enzyme, and the complex enzyme comprises cellulase and protease; the wall material of the earthworm enzymatic hydrolysate microcapsules comprises at least one of chitosan, calcium alginate, gelatin, and gum arabic; and the auxiliary agent comprises humic acid and inorganic natural minerals. The earthworm enzyme solution-containing soil conditioner, wherein the mass ratio of the cellulase and the protease is 1: (3-5).

[0008] The earthworm enzyme solution-containing soil conditioner, wherein the protease comprises one or more of papain, bromelin, trypsin, acid protease, neutral protease, and alkaline protease.

[0009] The earthworm enzyme solution-containing soil conditioner, wherein the mass ratio of the humic acid and the inorganic natural mineral is (1-2): 1.

[0010] The earthworm enzyme solution-containing soil conditioner, wherein the inorganic natural mineral comprises one or more of gypsum powder, zeolite powder, sepiolite, attapulgite, bentonite, and diatomite.

[0011] The earthworm enzyme solution-containing soil conditioner, wherein the particle size of the earthworm enzyme solution microcapsule and the auxiliary agent particle is 2-4 mm.

[0012] The second aspect of the present application provides a preparation method of a soil conditioner, which is used for preparing the earthworm enzyme solution-containing soil conditioner as described above, and comprises the following steps: S1. Preparing an earthworm enzyme solution: obtaining fresh earthworms, cleaning them, preparing a homogenate, then adding a compound enzyme, and performing an enzymatic reaction under the condition of 40-60 DEG C and 150-200 rpm, with a reaction time of 8-24 h, and with nitrogen being introduced for protection during the reaction process; after the enzymatic reaction is completed, the obtained hydrolysis product is filtered, the pH value of the filtrate is adjusted to 6-8, and an earthworm enzyme solution is obtained; S2. Microencapsulation embedding: the earthworm enzyme solution is added into a 0.01-0.03 g / mL sodium alginate solution, stirred uniformly, then an earthworm enzyme solution-containing sodium alginate solution is taken by using a sterile syringe, and is dropped into a 0.1-0.2 mol / L CaCl2 solution drop by drop to form a calcium alginate gel, which is hardened after being left to stand, and then is washed with distilled water to obtain a single-layer microcapsule; then the single-layer microcapsule is placed in a 0.005-0.02 g / mL chitosan solution for oscillation for 30-60 min, so as to prepare chitosan-calcium alginate double-layer microcapsule particles, which are freeze-dried to obtain earthworm enzyme solution microcapsules; S3. Preparing an auxiliary agent: mixing humic acid and an inorganic natural mineral, granulating, and drying to obtain auxiliary agent particles; S4. Mixing the earthworm enzyme solution microcapsules and the auxiliary agent particles to obtain the earthworm enzyme solution-containing soil conditioner.

[0013] The preparation method of the soil conditioner, wherein in S1, the addition amount of the compound enzyme is 1-2% (w / w).

[0014] The preparation method of the soil conditioner, wherein, in S2, the standing hardening time of the calcium alginate gel is 30-60 min.

[0015] The preparation method of the soil conditioner, wherein, in S2, the temperature of the freeze-drying is -40 to -20 DEG C, and the freeze-drying time is 10-20 h.

[0016] Beneficial effects: the soil conditioner containing earthworm enzymatic hydrolysate provided by the application adopts earthworm enzymatic hydrolysate microcapsules, and the earthworm enzymatic hydrolysate is wrapped by the microcapsules, so that the retention rate of active ingredients in the earthworm enzymatic hydrolysate can be significantly improved, the denaturation and inactivation of active substances such as amino acids and enzymes can be prevented, the shelf life is prolonged, there is no chemical residue, the environmental compatibility is good, the soil biological activity can be effectively increased, the soil activity can be stimulated, and the soil hardening can be quickly broken. The soil conditioner provided by the application is further used in combination with humic acid and inorganic natural minerals, so that the soil condition can be further improved, the crop yield can be improved, and the soil conditioner has a good application prospect. DETAILED DESCRIPTION

[0017] The application provides a soil conditioner containing earthworm enzymatic hydrolysate and a preparation method thereof, in order to make the purpose, technical scheme and effects of the application more clear and definite, the application is further described in detail in the following embodiments. It should be understood that the specific embodiments described herein are only used to explain the application and not to limit the application.

[0018] The application provides a soil conditioner containing earthworm enzymatic hydrolysate, which comprises the following components: earthworm enzymatic hydrolysate microcapsules and auxiliary agent particles in a mass ratio of (2-4):1; the earthworm enzymatic hydrolysate is prepared by enzymolysis of fresh earthworm homogenate through complex enzymes, and the complex enzymes comprise cellulase and protease; the wall material of the earthworm enzymatic hydrolysate microcapsules comprises at least one of chitosan, calcium alginate, gelatin and gum arabic; and the auxiliary agent comprises humic acid and inorganic natural minerals.

[0019] In the soil conditioner, the earthworm enzymatic hydrolysate is rich in various proteolytic enzymes, small molecule proteins, short peptides, free amino acids and other active ingredients, can stimulate soil microorganism reproduction, accelerate organic matter decomposition, promote the formation of granular structure, activate soil nutrients, and provide a good environment for crop growth. In the preparation of the earthworm enzymatic hydrolysate, the mild enzymolysis process is adopted, compared with the traditional earthworm hydrolysis process, the retention rate of active ingredients in the earthworm enzymatic hydrolysate can be significantly improved, and the problem that the active ingredients are prone to inactivation in the prior art is solved.

[0020] The microcapsule wall material can be specifically chitosan-calcium alginate compound. Chitosan is a natural high molecular linear polysaccharide after deacetylation of chitin, and a large number of hydrophilic groups such as hydroxyl and amino groups exist on the molecular chain, which has affinity and water retention to water, can retain water and fertilizer, reduce water and fertilizer loss, improve the drought resistance of crops, and at the same time, chitosan can be biodegraded, does not harden the soil in the soil, and can be slowly degraded into small molecular carbohydrates, which is harmless to the environment, and is an excellent ecological preparation. As a natural high molecular polysaccharide substance, calcium alginate can increase the content of soil organic matter, and can form stable organic-inorganic composite aggregates with clay minerals in the soil, improve the soil permeability and water retention capacity, and effectively reduce soil compaction. Chitosan-calcium alginate has good biocompatibility and film-forming property, can wrap the earthworm enzyme solution, avoid pollution, oxidation and decomposition of the earthworm enzyme solution. The microcapsule wall material can also use other natural high molecular material compounds such as gelatin-arabic gum compound, which has similar biocompatibility and film-forming property, and can realize the embedding of the enzyme solution.

[0021] The humic acid in the auxiliary agent is combined with soil particles through functional groups such as carboxyl and phenolic hydroxyl in the molecule, promotes the formation of aggregate structure, and increases the soil porosity; the inorganic natural mineral has strong adsorption property, can form aggregate structure by adsorbing soil particles, improve the soil permeability and water retention, especially for hardening or heavy clay soil, which is helpful to improve the soil fertility.

[0022] The humic acid, inorganic natural mineral and earthworm enzyme solution microcapsule can form a significant coordination effect in soil improvement, plant nutrition supply and biological stimulation, and the core is that the three can significantly improve the soil quality and plant growth performance through multi-dimensional cooperation such as structure complementation (physical action of inorganic natural mineral), nutrient regulation (chemical action of humic acid) and biological activation (biological action of earthworm enzyme solution).

[0023] Preferably, in the preparation raw material of the earthworm enzyme solution microcapsule, by weight, 60-70 parts of earthworm enzyme solution and 10-20 parts of wall material are included.

[0024] Preferably, the mass ratio of cellulase and protease is 1:(3-5). The combination of cellulase and protease has a synergistic effect, which can improve the enzyme hydrolysis efficiency. At the same time, the enzyme hydrolysis conditions of the two are relatively mild, which can better retain the active substances in the earthworm homogenate.

[0025] Preferably, the protease includes one or more of papain, bromelain, trypsin, acid protease, neutral protease and alkaline protease.

[0026] Preferably, the mass ratio of humic acid and inorganic natural mineral is (1-2):1.

[0027] Specifically, the mass ratio of humic acid to inorganic natural mineral and the type of inorganic natural mineral can be adjusted according to the fertility of different soils, so as to better play the role of the auxiliary agent.

[0028] Preferably, the inorganic natural mineral includes one or more of gypsum powder, zeolite powder, sepiolite, attapulgite, bentonite, and diatomite. Specifically, the attapulgite includes attapulgite clay and attapulgite stone.

[0029] Preferably, the particle size of the earthworm enzymatic hydrolysate microcapsule and the auxiliary agent particle is 2-4 mm.

[0030] The second aspect of the present application provides a preparation method of a soil conditioner, which is used for preparing the earthworm enzymatic hydrolysate-containing soil conditioner as described above, and includes the following steps: S1. Preparing earthworm enzymatic hydrolysate: fresh earthworms are obtained, washed clean, and prepared into a homogenate, and then a compound enzyme is added, and an enzymatic hydrolysis reaction is carried out under the condition of 40-60℃ and 150-200 rpm, and the reaction time is 8-24 h, and nitrogen is introduced for protection during the reaction process, and after the enzymatic hydrolysis reaction is completed, the obtained hydrolysis product is filtered, and the pH value of the filtrate is adjusted to 6-8, so as to obtain the earthworm enzymatic hydrolysate; S2. Microencapsulation embedding: the earthworm enzymatic hydrolysate is added into a 0.01-0.03 g / mL sodium alginate solution, stirred uniformly, and then a sterile syringe is used to suck the sodium alginate solution containing the earthworm enzymatic hydrolysate, and the sodium alginate solution is dropped into a 0.1-0.2 mol / L CaCl2 solution drop by drop, so as to form a calcium alginate gel, and the calcium alginate gel is hardened after being placed and hardened, and then the single-layer microcapsule is obtained after being washed with distilled water, and then the single-layer microcapsule is placed in a 0.005-0.02 g / mL chitosan solution for oscillation for 30-60 min, so as to obtain chitosan-calcium alginate double-layer microcapsule particles, and the chitosan-calcium alginate double-layer microcapsule particles are freeze-dried, so as to obtain the earthworm enzymatic hydrolysate microcapsule; In this step, the microencapsulation embedding is combined with the freeze-drying technology, the natural chitosan-calcium alginate compound is used as the wall material for the microencapsulation embedding, so as to avoid the use of chemical cross-linking agents, the structure of the microcapsule can be guaranteed to be stable by using the freeze-drying technology, the particles are uniform, and the storage period of the microcapsule and its contents is further improved, so as to solve the problems of short action period of the enzymatic hydrolysate and environmental risk in the use process of the microcapsule in the prior art; S3. Preparing an auxiliary agent: humic acid and inorganic natural mineral are mixed, granulated, and dried, so as to obtain the auxiliary agent particle; S4. Mixing the earthworm enzymatic hydrolysate microcapsule and the auxiliary agent particle, so as to obtain the earthworm enzymatic hydrolysate-containing soil conditioner.

[0031] Preferably, in S1, the addition amount of the compound enzyme is 1-2% (w / w). If the addition amount of the compound enzyme is too much, the enzymatic hydrolysis will be too strong, and the retention rate of the active ingredient will be low.

[0032] Preferably, in S2, the standing hardening time of the calcium alginate gel is 30-60 min.

[0033] Preferably, in S2, the temperature of freeze-drying is -40 to -20℃, and the freeze-drying time is 10-20 h.

[0034] Preferably, in S3, a disc granulator is used for granulation.

[0035] Preferably, the parameters of the disc granulator are set as follows: disc rotation speed 15-20 rpm, disc angle 60 degrees, spray flow rate 50-100 mL / min, and distance between scraper and disc bottom 2-4 mm.

[0036] The following examples and comparative examples further illustrate the present application.

[0037] Example 1 A soil conditioner composed of microcapsules of earthworm enzymatic hydrolysate and granules of auxiliary agent at a mass ratio of 2:1; The preparation method is as follows: S1. Preparation of earthworm enzymatic hydrolysate After fresh earthworms are washed and homogenized, 0.02 g / mL of a complex enzyme solution is added, and the mixture is stirred uniformly. In the complex enzyme, the mass ratio of protease to cellulase is 3:1, and the protease is composed of papain and acid protease at a mass ratio of 2:1. The earthworm homogenate and the complex enzyme are poured into a fermentation tank for enzymatic hydrolysis at a reaction temperature of 50℃ for 12 h at a stirring speed of 150 rpm, and nitrogen is introduced throughout the reaction process for protection. After the enzymatic hydrolysis reaction is completed, the hydrolysate is filtered, and the pH of the filtrate is adjusted to 7 to obtain the earthworm enzymatic hydrolysate. S2. Microencapsulation treatment The earthworm enzymatic hydrolysate is added to 0.02 g / mL of a sodium alginate solution at a mass ratio of 5:1, and the mixture is stirred uniformly. The above-mentioned sodium alginate solution containing the earthworm enzymatic hydrolysate is dropped into 0.2 mol / L of a CaCl2 solution using a sterile syringe to form a calcium alginate gel, with each drop being 0.1-0.2 mL. The gel is allowed to stand for 30 min for hardening, and then it is washed with distilled water to obtain single-layer microcapsules. The single-layer microcapsules obtained by the above-mentioned method are placed in 0.02 g / mL of a chitosan solution for oscillation for 30 min to allow slow film formation, thereby preparing chitosan-calcium alginate double-layer microcapsule granules. The chitosan-calcium alginate double-layer microcapsule granules are subjected to freeze-drying treatment at a temperature of -35℃ for 16 h to obtain dried earthworm enzymatic hydrolysate microcapsules with a particle size of 2-4 mm. S3. Preparation of auxiliary agent The humic acid and zeolite powder are mixed in a mass ratio of 2:1, stirred uniformly, and then the mixture is placed in a disc granulator for granulation, with a disc rotation speed of 15 rpm, a disc angle of 60 degrees, a spraying flow of 50 mL / min, a distance between the scraper and the disc bottom of 3 mm, and drying treatment after granulation, to obtain the granules of the auxiliary agent with a particle size of 2-4 mm; S4. The earthworm enzyme hydrolysate microcapsules and the auxiliary agent granules are mixed in a mass ratio of 2:1 to obtain the soil conditioner containing the earthworm enzyme hydrolysate.

[0038] Example 2 A soil conditioner, the preparation method of which is different from that of Example 1 in that, in the preparation of the earthworm enzyme hydrolysate, the mass ratio of the protease to the cellulase in the compound enzyme is 5:1, and the protease is composed of papain, neutral protease and bromelain, with a mass ratio of 2:1:2.

[0039] Example 3 A soil conditioner, the preparation method of which is different from that of Example 1 in that, in S2, the wall material for microencapsulation treatment is 0.01 g / mL of sodium alginate solution, 0.1 mol / L of CaCl2 solution and 0.01 g / mL of chitosan solution.

[0040] Example 4 A soil conditioner, the preparation method of which is different from that of Example 1 in that, in S2, after the addition of the CaCl2 solution, the gel is hardened for 60 min, and the single-layer microcapsules are oscillated in the chitosan solution for 60 min.

[0041] Example 5 A soil conditioner, the preparation method of which is different from that of Example 1 in that, in S3, the auxiliary agent is a mixture of humic acid and inorganic natural minerals (zeolite powder + diatomite) in a mass ratio of 1:1, and the mass ratio of the zeolite powder to the diatomite is 1:1.

[0042] Example 6 A soil conditioner, the preparation method of which is different from that of Example 1 in that, it is composed of earthworm enzyme hydrolysate microcapsules and auxiliary agent granules in a mass ratio of 4:1.

[0043] Comparative Example 1 A soil conditioner, the preparation method of which is different from that of Example 1 in that, in S1, no cellulase is used, and the protease is composed of papain, acid protease and bromelain in a mass ratio of 2:1:2.

[0044] Comparative Example 2 A soil conditioner, the preparation method of which is different from that of Example 1 in that, in S1, no nitrogen gas is introduced into the fermenter during the preparation of the earthworm enzymatic hydrolysate.

[0045] Comparative Example 3 A soil conditioner, the preparation method of which is different from that of Example 1 in that, in S2, the wall material is microencapsulated using a 0.02 g / mL sodium alginate solution and a 0.2 mol / L CaCl2 solution, and no chitosan is used for secondary coating.

[0046] Comparative Example 4 A soil conditioner, the preparation method of which is different from that of Example 1 in that, in the preparation of the auxiliary agent particles, only humic acid is used, and no inorganic natural mineral is compounded.

[0047] Comparative Example 5 A soil conditioner, the preparation method of which is different from that of Example 1 in that, in the preparation of the auxiliary agent particles, gypsum powder and bentonite are used in a mass ratio of 1:1, and no humic acid is compounded.

[0048] Comparative Example 6 A soil conditioner, which is composed only of earthworm enzymatic hydrolysate microcapsules.

[0049] Test 1: Amino acid and enzyme activity test 5 g of the soil conditioner prepared in the examples or comparative examples is weighed into 50 mL of water, and the microcapsule structure is destroyed by ultrasonic treatment to form a suspension containing earthworm enzymatic hydrolysis amino acids. The amino acid concentration in the suspension is determined by using an automatic amino acid analyzer, and the enzyme activity is determined by using a fibrin plate method. The free amino acid and enzyme activity in the earthworm enzymatic hydrolysate stock solution are also detected, and the preservation efficiency of the amino acid and enzyme after microcapsule treatment is calculated.

[0050] Table 1: Preservation rates of active enzymes and free amino acids in examples and comparative examples

[0051] As can be seen from the data in Table 1, the soil conditioners of Examples 1-6 and Comparative Examples 4-6 have very excellent preservation effects on active enzymes and free amino acids; the preservation rates of active enzymes and free amino acids of the soil conditioners of Comparative Examples 1-3 are significantly reduced, indicating that the use of protease and cellulase in the earthworm enzymatic hydrolysis process, the nitrogen protection treatment during the enzymatic hydrolysis process, and the chitosan secondary coating treatment can all improve the preservation rates of active enzymes and free amino acids in the conditioner, and the chitosan secondary coating treatment has the best effect on the preservation rates of active enzymes and free amino acids. The auxiliary agent has little effect on the preservation rates of active enzymes and free amino acids.

[0052] Test 2: Amino acid release rate test The soil samples were collected, air-dried and mixed, 100 g of soil was placed in a 200 mL beaker, 10 g of soil amendment prepared in the examples or comparative examples was mixed with the test soil, the soil humidity was kept at 60% to 70%, and the mixture was placed at room temperature. After 1, 10, 20, 40 and 60 days, the test soil was extracted with aqueous solution, the amino acid concentration in the extract was determined by using a full-automatic amino acid instrument, the enzyme activity in the extract was determined by using a fibrin plate method, and the free amino acid and enzyme activity in the original soil and earthworm enzyme solution were detected, and the release rate of the active enzyme and free amino acid in each treatment was calculated.

[0053] Table 2: Release rate of active enzyme and free amino acid in examples and comparative examples

[0054] As shown in Table 2, after 60 days of application in soil, the release rates of active enzyme and free amino acid in the soil amendments of examples 1 to 6 and comparative examples 4 to 6 were relatively high, and the release rates of active enzyme and free amino acid in comparative examples 1 to 3 were relatively low. The same rule applied to the preservation rate of active enzyme and free amino acid. In comparative example 3, chitosan was not used for secondary coating, and the active enzyme and free amino acid were rapidly released after application in soil. Due to the low preservation rate of active enzyme and free amino acid, the release rate was also low in the later period.

[0055] Test 3: Soil improvement effect test The soil samples were collected, air-dried and mixed, 2 kg of soil was placed in a culture pot, 100 g of soil amendment prepared in the examples or comparative examples was mixed with the test soil, the soil humidity was kept at 60% to 70%, and the mixture was placed at room temperature. The soil improvement experiment was started, and the soil samples were collected after 2 months. The soil organic matter was determined by using potassium dichromate external heating method, and the soil bulk density was determined by using a cutting ring method. Another control test was added, in which no soil amendment was applied. The change rates of organic matter and bulk density of the soil in each treatment and the control were calculated.

[0056] Table 3: Influence of examples and comparative examples on soil organic matter and bulk density

[0057] As shown in Table 3, after 60 days of application in soil, the soil amendment of examples 1 to 6 had excellent improvement effects on soil organic matter and bulk density. Although comparative examples 1 to 4 improved the soil organic matter, they had poor improvement effect on bulk density. Comparative examples 5 to 6 did not have good improvement effect on soil organic matter and bulk density due to the absence of auxiliary agent humic acid.

[0058] It can be understood that, for those skilled in the art, equivalent replacements or changes can be made according to the technical solutions and inventive concepts of the present application, and all these changes or replacements shall fall within the protection scope of the appended claims of the present application.

Claims

1. A soil conditioner comprising earthworm enzyme lysate, characterized in that, The soil improver comprises the following components: earthworm enzymatic hydrolysate microcapsules and auxiliary agent granules, with a mass ratio of (2-4):

1. The earthworm enzymatic hydrolysate is prepared by enzymatic hydrolysis of fresh earthworm homogenate using a complex enzyme, wherein the complex enzyme comprises cellulase and protease. The wall material of the earthworm enzymatic hydrolysate microcapsules comprises at least one of chitosan, calcium alginate, gelatin, and gum arabic. The auxiliary agent comprises humic acid and inorganic natural mineral.

2. The earthworm enzyme-containing lysate soil amendment of claim 1, wherein, The mass ratio of the cellulase and the protease is 1:(3-5).

3. The earthworm enzyme-containing lysate soil amendment of claim 1, wherein, The protease comprises one or more of papain, bromelain, trypsin, acid protease, neutral protease, and alkaline protease.

4. The earthworm enzyme-containing lysate soil amendment of claim 1, wherein, The mass ratio of the humic acid and the inorganic natural mineral is (1-2):

1.

5. The earthworm enzyme-containing lysate soil amendment of claim 1, wherein, The inorganic natural mineral comprises one or more of gypsum powder, zeolite powder, sepiolite, attapulgite, bentonite, and diatomite.

6. The earthworm enzyme-containing lysate soil amendment of claim 1, wherein, The particle size of the earthworm enzymatic hydrolysate microcapsules and the auxiliary agent granules is 2-4 mm.

7. A method for preparing a soil conditioner for preparing a soil conditioner containing earthworm proteolytic fluid as claimed in any one of claims 1 to 6, characterized in that, The soil improver comprises the following steps: S1. Preparation of earthworm enzymatic hydrolysate: fresh earthworms are obtained, washed clean, and prepared into homogenate, and then a complex enzyme is added, and enzymatic hydrolysis reaction is carried out at 40-60°C and 150-200 rpm, with a reaction time of 8-24 h, and nitrogen is introduced during the reaction process for protection, and after the enzymatic hydrolysis reaction is completed, the obtained hydrolysis product is filtered, and the pH value of the filtrate is adjusted to 6-8, to obtain earthworm enzymatic hydrolysate; S2. Microencapsulation embedding: the earthworm enzymatic hydrolysate is added to a 0.01-0.03 g / mL sodium alginate solution, stirred uniformly, and then a sterile syringe is used to suck the sodium alginate solution containing the earthworm enzymatic hydrolysate, and the solution is dropped into a 0.1-0.2 mol / L CaCl2 solution drop by drop to form a calcium alginate gel, which is allowed to harden after standing, and then the hardened single-layer microcapsules are obtained after being washed with distilled water, and then the single-layer microcapsules are placed in a 0.005-0.02 g / mL chitosan solution for oscillation for 30-60 min, to obtain chitosan-calcium alginate double-layer microcapsule granules, which are freeze-dried to obtain earthworm enzymatic hydrolysate microcapsules; S3. Preparation of auxiliary agent: humic acid and inorganic natural mineral are mixed, granulated, and dried to obtain auxiliary agent granules; S4. The earthworm enzymatic hydrolysate microcapsules and the auxiliary agent granules are mixed to obtain the soil improver containing earthworm enzymatic hydrolysate.

8. The method of claim 7, wherein the soil amendment is prepared by, In S1, the addition amount of the complex enzyme is 1-2% (w / w).

9. The method of claim 7, wherein the soil amendment is prepared by, In S2, the standing hardening time of the calcium alginate gel is 30-60 min.

10. The method of claim 7, wherein the soil amendment is prepared by, In S2, the temperature for freeze-drying is -40 to -20°C, and the freeze-drying time is 10-20 h.

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