Systematic evaluation method for oral fat composition containing natural extract

Abstract

The invention relates to the field of cosmetic testing, in particular to a systematic evaluation method of a natural avocado extract-containing mouth fat composition, which comprises the following steps: mixing pulp with an extraction solvent, condensing and refluxing for 14-18 hours to obtain an avocado extract, adding allantoin for corrosion prevention, and storing; the fixed substrate comprises the following components: beeswax, paraffin, coconut oil, lake, lemon oil, vanillin, glyceryl monostearate, titanium dioxide, castor oil, wool fat and isopropyl palmitate, and an avocado extract is added to form a contrast formula; a multi-dimensional performance test pack is adopted, a non-linear correlation model is established, and the optimal adding proportion of the adding amount of the avocado extract is determined; and standardized testing. According to the method, three technical problems of fragmentation of evaluation dimensions, insufficient parameter association and lack of method standardization in the prior art are solved through a full-process framework of directional extraction, gradient formula, multi-dimensional test, parameter association and standardization specification.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetic evaluation methods, and more particularly to a systematic evaluation method for a lip balm composition containing natural extracts. BACKGROUND

[0002] As widely used cosmetics, lip balm compositions are of great concern due to their direct contact with the mucous membrane of the lips. Traditional synthetic lip balm compositions rely on ingredients such as paraffin, mineral oil, and synthetic pigments, but long-term use may introduce harmful substances such as lead, p-hydroxybenzoic acid esters, and oleic acid alcohol. Studies have shown that the detection rate of lead in commercially available lip balm compositions is as high as 62%, and the lead content of some products exceeds the safety limit by 3 times. The daily intake of p-hydroxybenzoic acid esters can reach 0.1 mg / kg, and 30% of the subjects have allergic reactions to lip balm compositions containing 10% oleic acid alcohol. In addition, residual particles of synthetic pigments may cause minor damage to the lips.

[0003] With the increasing demand for natural and safe products among consumers, natural ingredients such as avocado oil and purple yam extract have gradually become the core raw materials for lip balm composition formulations. For example, lip balm compositions prepared from purple yam extract have high melting points, high hardness, and stable pH values, and their antioxidant activity is significantly better than that of synthetic ingredients. Similarly, pomegranate extract and lycopene also demonstrate the advantages of natural extracts.

[0004] However, the existing technology has significant limitations: first, research focuses on a single link or indicator, and lacks a comprehensive evaluation system. For example: the study of purple yam lip balm composition only tests physical properties, without analyzing the impact of extraction process on pigment stability; the beetroot juice lip balm composition does not compare the impact of extraction temperature on pigment stability, resulting in a color difference ΔE of 5.2; the dragon fruit extract lip balm composition only tests antibacterial properties, without analyzing the impact of extraction liquid pH on formula stability.

[0005] Secondly, in the purple yam lip balm composition, the correlation between high melting point and extract concentration, wax compounding, and castor oil content is not clear; when beeswax is compounded with avocado oil, the impact mechanism of the ratio on melting point and hardness is not quantified; the synergistic effect of the ratio of avocado oil and dragon fruit seed oil on heat resistance is not analyzed, resulting in an unclear optimal addition ratio.

[0006] Thirdly, the melting point test methods differ greatly; hardness data is not comparable; stability testing only observes changes at room temperature for 30 days, without following the 6-month 40°C / 75%RH accelerated test required by the ICH guidelines, and cannot predict long-term shelf life.

[0007] For example, Chaudhari et al. (2019) did not quantify the effect of extraction temperature on pigment stability when preparing a lip balm composition with beetroot juice, resulting in a finished product with a color difference ΔE of 5.2; whereas the present invention controls ΔE ≤ 3.5 through standardized lighting tests, significantly improving color stability.

[0008] In summary, the prior art relies on empirical adjustments and there is an urgent need to establish a systematic evaluation method covering the entire process of "extraction process optimization-formulation ingredient synergy-finished product performance verification" and multiple dimensions of indicators to solve the problems of dispersed evaluation, unclear parameter correlation and lack of standardization. SUMMARY

[0009] To solve the above technical problems in the prior art, the present invention provides a systematic evaluation method for a lip balm composition containing natural avocado extract, comprising the following steps:

[0010] (1) Directional extraction of active ingredients from avocados: mix the pulp and extraction solvent at a mass ratio of 1:(1.8-2.2), condense and reflux at 75-85°C for 14-18 hours to obtain avocado extract, add 0.5-1.5% allantoin for preservation and store at 2-8°C;

[0011] (2) Add 0-10% avocado extract to a fixed matrix, which includes beeswax 6-10%, paraffin 15-20%, coconut oil 6-10%, lake 3-5%, lemon oil 0.01-0.03%, vanilla extract 0.8-1.2%, monostearin 4-6%, titanium dioxide 1-3%, castor oil 40-48%, lanolin 4-6%, isopropyl palmitate 8-12%, to form a comparative formulation;

[0012] (3) Multi-dimensional performance testing includes: melting point determination, hardness testing, homogeneity testing, uniformity testing, and stability testing;

[0013] (4) Parameter correlation analysis: establish a non-linear correlation model to determine the optimal addition ratio of avocado extract addition amount;

[0014] (5) Standardized testing.

[0015] The extraction solvent in step (1) is ethyl acetate, ethanol or n-hexane, the extraction temperature is 65-90°C, and the time is 10-24 hours.

[0016] The fixed matrix in step (2) is a wax matrix, carnauba wax or candelilla wax, with a melting point range of 55-90°C and a hardness breaking load of 80-300g.

[0017] The melting point was determined using the capillary method; the hardness was tested using a cantilever beam fracture test; the homogeneity test involved coating the lip balm composition onto a polydimethylsiloxane film and detecting the transmittance using an ultraviolet spectrophotometer; and the uniformity test involved observing the distribution of lip granules under a microscope and calculating the coefficient of variation.

[0018] The homogeneity test uses a laser particle size analyzer or a colorimeter; the hardness test uses a weight loading method, and a texture analyzer can be selected in parallel.

[0019] The colorimeter measures a color difference value ΔE ≤ 3.5 after illumination.

[0020] The texture analyzer method has a trigger force of 5-15g and an accuracy deviation of ≤10g.

[0021] The optimal addition ratio of avocado extract in step (4) is 5-7%, with corresponding performance indicators as follows: melting point 65-72℃, hardness 200-280g, uniformity coefficient of variation CV≤25%, and pH value 5.5-6.2.

[0022] The optimal addition ratio of avocado extract resulted in an adhesion strength of 0.001-0.004 g (measured by the tape adhesion method).

[0023] The present invention has the following advantages over the prior art:

[0024] This invention, by integrating a comprehensive evaluation framework encompassing "extraction process optimization, formulation gradient design, multi-dimensional performance testing, and parameter correlation analysis," achieves for the first time a systematic evaluation of lip balm compositions containing natural extracts, from raw material preparation to finished product performance. This method covers key dimensions such as physical properties, chemical properties, performance characteristics, and safety, overcoming the limitations of existing technologies that focus only on a single step or indicator, and providing a comprehensive scientific basis for the development of natural lip balm compositions.

[0025] Through gradient formulation design and parameter correlation analysis, this invention clarifies the synergistic mechanism between avocado oil and matrix components, and analyzes their nonlinear influence on finished product performance (such as hardness, uniformity, and heat resistance). This model can accurately identify the optimal addition ratio, solving the problem of formulation blindness caused by insufficient parameter correlation in existing technologies.

[0026] This invention standardizes key testing procedures (such as capillary method for melting point measurement, cantilever beam method for hardness measurement, and ultraviolet spectrophotometry for uniformity assessment) and strictly follows international standards, significantly improving the reliability and cross-study comparability of data. It overcomes the problem of data unavailability caused by methodological differences in existing technologies and provides a replicable technical benchmark for industrial quality control.

[0027] The proposed evaluation method supports flexible replacement of extraction solvents, wax matrices, and testing instruments. It can also predict the performance of compound ingredients through machine learning models and is compatible with the expansion of bioefficacy and sensory evaluation, reserving technical space for the continuous innovation of natural oral balm compositions. Attached Figure Description

[0028] Figure 1 This is an experimental diagram of the melting point determination of this invention;

[0029] Figure 2 This is an experimental diagram showing the hardness test of the oral balm composition of the present invention;

[0030] Figure 3 This is an experimental diagram of the homogeneity test of this invention;

[0031] Figure 4 This is an experimental diagram of the uniformity test of this invention;

[0032] Figure 5 This is an experimental diagram of the friction force of this invention;

[0033] Figure 6 This is an experimental diagram showing the adhesion of the present invention;

[0034] Figure 7 This is an experimental diagram showing the melting point of the oral balm with different amounts of avocado extract added according to the present invention.

[0035] Figure 8 This is an experimental diagram showing the hardness of lip balm with different amounts of avocado extract added in this invention. Figure 9 This is an integrated sphere test image of lip balm with different amounts of avocado extract added in this invention; Figure 10 These are microscopic images of oil particles in liposomes with different amounts of avocado extract added in this invention; Figure 11 The Pb of the M04 sample in this invention 2+ Determination of lead ion content in heavy metal; Figure 12 The Cu in the M04 sample of this invention 2+ Determination of lead ion content in heavy metal; Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of this invention clearer, the invention is described below with reference to specific embodiments shown in the accompanying drawings. However, it should be understood that these descriptions are merely exemplary and not intended to limit the scope of the invention. Furthermore, descriptions of well-known structures and technologies are omitted in the following description to avoid unnecessarily obscuring the concept of the invention.

[0037] This invention provides a systematic evaluation method for a lip balm composition containing natural avocado extract, comprising the following steps: (1) directional extraction of avocado active ingredients: mixing the pulp and extraction solvent at a mass ratio of 1:(1.8-2.2), refluxing at 75-85℃ for 14-18 hours to obtain an avocado extract with a yield ≥8%, adding 0.5-1.5% allantoin for preservation and storing at 2-8℃; (2) fixing the matrix components including beeswax 6-10%, paraffin 15-20%, coconut oil 6-10%, lake 3-5%, and lemon oil 0. 0.01-0.03%, vanilla extract 0.8-1.2%, monostearate glycerides 4-6%, titanium dioxide 1-3%, castor oil 40-48%, lanolin 4-6%, isopropyl palmitate 8-12%, and 0-10% avocado extract were added to form a comparative formula; (3) Multi-dimensional performance tests included: melting point determination, hardness test, homogeneity test, uniformity test, and stability test; (4) Parameter correlation analysis: a nonlinear correlation model was established to determine the optimal addition ratio of avocado extract; (5) Standardization test.

[0038] In step (1), the extraction solvent is ethyl acetate, ethanol, or n-hexane, the extraction temperature is 65-90℃, and the time is 10-24 hours. The extraction time can be adjusted according to the target active ingredient, such as n-hexane preferred for fatty acids and ethanol preferred for vitamin E, maintaining a pulp-to-solvent mass ratio of 1:2.

[0039] In step (2), the fixed matrix is ​​a wax matrix, carnauba wax or candelilla wax, with a melting point range of 55-90℃ and a hardness fracture load of 80-300g.

[0040] The melting point was determined using the capillary method; the hardness was tested using a cantilever beam fracture test; the homogeneity test involved coating the lip balm composition onto a polydimethylsiloxane film and detecting the transmittance using an ultraviolet spectrophotometer; and the uniformity test involved observing the distribution of lip granules under a microscope and calculating the coefficient of variation.

[0041] The homogeneity test uses a laser particle size analyzer or a colorimeter; the hardness test uses a weight loading method, and a texture analyzer can be selected in parallel.

[0042] The colorimeter measures a color difference value ΔE ≤ 3.5 after illumination.

[0043] The texture analyzer method has a trigger force of 5-15g and an accuracy deviation of ≤10g.

[0044] The optimal addition ratio of avocado extract in step (4) is 5-7%, with corresponding performance indicators as follows: melting point 65-72℃, hardness 200-280g, uniformity coefficient of variation CV≤25%, and pH value 5.5-6.2.

[0045] The optimal addition ratio of avocado extract resulted in an adhesion strength of 0.001-0.004 g (measured by the tape adhesion method).

[0046] Example 1

[0047] 1) Targeted extraction of active ingredients from avocado: Ethyl acetate was used as the extraction solvent (pulp to solvent mass ratio 1:2). The mixture was refluxed overnight at 80℃, and concentrated by diatomaceous earth filtration and rotary evaporation to obtain avocado extract with a yield ≥10% (containing 71% monounsaturated fatty acids, 13% polyunsaturated fatty acids, vitamin E and phytosterols). 1% allantoin was added for preservation and refrigerated (2-8℃).

[0048] 2) Formulation Design: The base components were fixed at 8% beeswax, 18% paraffin, 8% coconut oil, 4% lake, 0.02% lemon oil, 1% vanilla extract, 5% monostearate, 2% titanium dioxide, 44.80% castor oil, 5% lanolin, and 10% isopropyl palmitate. Avocado extract was added at different amounts (0%, 4%, 6%, 8%, and 10%) to form five comparative formulations (M01-M05).

[0049] 3) Multi-dimensional performance testing: Melting point determination (using capillary method, 2mm inner diameter × 7cm long capillary, water bath heating rate 1℃ / min, recording the initial melting to full melting temperature); Hardness test (cantilever beam fracture test, the lip balm composition is suspended for 12.1mm, 10g gradient loading until fracture); Uniformity evaluation (observing the distribution of oil particles under a microscope at 40×0.25x magnification, calculating the coefficient of variation CV; detecting the transmittance of 400nm blue light with a UV spectrophotometer, the transmittance is the highest at 8% addition); pH value determination (1.000g of lip balm composition is dissolved in an ethanol / water (v:v 3:1) mixture, filtered and measured with a pH meter, pH = 5.95-5.99 when avocado extract is added at 6% and 8% addition); Heavy metal detection (flame atomic absorption spectrometry, lead and copper content are both ≤1mg / kg); Adhesion test (adhesive tape adhesion method, weighing the adhered mass);

[0050] 4) Parameter correlation analysis: A nonlinear correlation model of "avocado extract concentration - matrix compatibility - performance index" was established based on experimental data. The optimal addition amount of avocado extract was 6% (melting point 69℃, hardness 250g, CV=21.5%, pH=5.95, and optimal adhesion).

[0051] 5) Standardized testing: Standardize the operating procedures and instrument parameters for each test, including: homogeneity testing using a UV spectrophotometer (185-900nm wavelength) or microscopic observation; pH testing using an ethanol / water (3:1) mixture dissolved and filtered for measurement; melting point determination using the capillary method (water bath heating rate 1℃ / min); hardness testing using a fixed cantilever length (12.1mm); and stability testing following the ICH guidelines (40℃ / 75%RH accelerated test).

[0052] Example 2

[0053] Ethanol was used as the extraction solvent (pulp:solvent = 1:2), and the mixture was refluxed at 70°C for 14 hours to obtain an extract with a yield of 9% (vitamin E content increased by 15%). Carnauba wax (melting point 82°C) was used to replace beeswax, and other matrix components were the same as in Example 1. Avocado extract was added in gradients of 0%, 4%, 6%, 8%, and 10%. At a 6% addition, the hardness reached 270g (cantilever beam method) and the melting point was 71°C (capillary method). The compatibility of the wax matrix replacement was verified.

[0054] Example 3

[0055] The fixed matrix components include 6% beeswax, 15% paraffin, 6% coconut oil, 3% lake, 0.01% lemon oil, 0.8% vanilla extract, 4% monostearate, 1% titanium dioxide, 40% castor oil, 4% lanolin, and 8% isopropyl palmitate. Other steps are the same as in Example 1.

[0056] Example 4

[0057] The fixed matrix components include 10% beeswax, 20% paraffin, 10% coconut oil, 5% lake, 0.03% lemon oil, 1.2% vanilla extract, 6% monostearate, 3% titanium dioxide, 48% castor oil, 6% lanolin, and 12% isopropyl palmitate. Other steps are the same as in Example 1.

[0058] Example 5

[0059] In this embodiment, the mass ratio of fruit pulp to solvent is 1:1.8, and the other steps are the same as in Example 1.

[0060] Example 6

[0061] In this embodiment, the mass ratio of fruit pulp to solvent is 1:2.2, and the other steps are the same as in Example 1.

[0062] Example 7

[0063] In this embodiment, step (2) involves fixing the matrix components as follows: beeswax 6-10%, paraffin 15-20%, coconut oil 6-10%, lake 3-5%, lemon oil 0.01-0.03%, vanilla extract 0.8-1.2%, monostearate 4-6%, titanium dioxide 1-3%, castor oil 40-48%, lanolin 4-6%, and isopropyl palmitate 8-12%. Then, 0-10% avocado extract and 0.5% acetone tetrahydropyranol are added to form a comparative formulation. Other steps are the same as in Example 1.

[0064] In the multi-dimensional performance test, the homogeneity test can be replaced by a laser particle size analyzer (to directly measure the particle size distribution of oil particles) or a colorimeter (to measure the color difference value ΔE≤3.0 after illumination), and the hardness test can be replaced by a texture analyzer (trigger force 10g, accuracy ±5g), but it must be ensured that the test results can be cross-validated with the original method data.

[0065] In the parameter correlation analysis, the nonlinear correlation model established using experimental data (melting point 64-69℃, hardness 100-250g, CV = 21.5-29.7%, maximum transmittance 8%, pH = 5.95-5.99) can be extended to predict the formulation performance of other natural extracts (such as pomegranate extract and lycopene) (e.g., antioxidant activity increased to 90% DPPH scavenging rate).

[0066] This invention effectively overcomes three major technical problems in the background technology—"fragmented evaluation dimensions," "insufficient correlation of key parameters," and "lack of standardized evaluation methods"—through techniques such as extraction process optimization, formulation gradient design, multi-dimensional performance testing, and parameter correlation analysis. Specific experimental methods and results are as follows:

[0067] 1) Experimental methods

[0068] a. Physical property testing:

[0069] Melting point determination: Use a capillary tube with an inner diameter of 2 mm and a length of 7 cm, filled to a height of 2-3 mm, heat in a water bath using a B-shaped tube, and record the initial melting and complete melting temperatures. (See below) Figure 1 , 7 The 6% addition group had a peak melting point of 69℃, which was 7.8% higher than the 0% group (64℃).

[0070] Hardness test: The lip balm composition was suspended halfway above the table to form a cantilever, and a 10g gradient load was applied until it broke.

[0071] (as follows Figure 2 )

[0072] Homogeneity test: Six pieces of polydimethylsiloxane film (PDMS, DC20) were cut and coated with lip balm compositions containing different avocado proportions: 0% (M01), 4% (M02), 6% (M03), 8% (M04), and 10% (M05). The transmittance was measured using a UV spectrophotometer (400nm). (See below) Figure 3 ).

[0073] Table 1 - Application Amount of PDMS Lip Gloss Composition

[0074] M01 M02 M03 M04 M05 Thin film / g 0.20836 0.22299 0.21644 0.21310 0.22008 Thin film + lip balm composition / g 0.22525 0.22727 0.22329 0.22526 0.23097 Lip balm composition / g 0.01689 0.00428 0.00685 0.01216 0.01089

[0075] Uniformity Test: 0.08000 mg, 0.04000 mg, 0.67000 mg, 0.12000 mg, and 0.09000 mg of oral balm compositions M01 to M05 were taken respectively, placed on a glass slide, and pressed evenly with a coverslip to form a thin, round dot. The distribution was observed under a biological microscope at magnifications of 4×0.25, 10×0.25, 20×0.25, and 40×0.25. Ten oil particles were selected from the images at 40×0.25 magnification for uniformity analysis based on particle size. (See below) Figure 4 )

[0076]

[0077] CV: Coefficient of variation; SD: Standard deviation; Mean: U

[0078] Table 2 - Sampling Amount of Lip Gloss Composition on Glass Slide

[0079]

[0080] b. Chemical property testing:

[0081] pH value determination: Dissolve 1.000g of the oral balm composition in an ethanol / water (3:1) mixture and measure the pH value using a pH meter.

[0082] Heavy metal detection: Five portions of the oral balm composition (1.000 g each) were dissolved in dichloromethane (50.00 mL), extracted with sulfuric acid (1.00 mol / L, 25.00 mL), and the supernatant was poured into a volumetric flask. Five portions of copper and lead standard solutions and sample solutions of different concentrations (0.09 μg / mL, 1.20 μg / mL, 1.50 μg / mL, 1.80 μg / mL, 2.10 μg / mL) were taken and determined using an atomic absorption spectrophotometer (4510GF). Figures 11-12 The atomic absorption standard curve showed that the highest lead content was 0.7125 μg / L (far below the national standard of 1 mg / kg).

[0083] c. Use performance testing:

[0084] Friction test: Pull the strip of paper containing the lip balm composition and record the reading. (See below) Figure 5 )

[0085] Adhesion test: Cut five strips of tape of equal length and width. Apply each of the five lip gloss compositions evenly to your hand, adhere them to the tape, and weigh the mass of the lip gloss composition adhering to the tape. Evaluate the adhesive strength of the lip gloss composition. (See below) Figure 6 )

[0086] Table 3 - Adhesive Tape Adhesive Composition Amount

[0087]

[0088] d. Safety and stability testing:

[0089] Skin irritation test: Apply to arm and observe after 15-20 minutes.

[0090] Stability test: Observe the changes in color, appearance and odor at room temperature (25℃) for 7 days.

[0091] 2) Experimental Results

[0092] a. Physical property testing:

[0093] Melting point determination: The heat resistance of the lip balm composition was quantified. The melting point was 69°C at 6% addition, which was significantly higher than 64°C at 0% and 58°C at 10%.

[0094] Hardness test: Quantitative structural strength, the fracture load at 6% addition is 250g, which is 1.47 times that of the 0% group (170g).

[0095] Homogeneity test: The transmittance is highest at an addition of 6%, indicating that the composition is uniform.

[0096] Uniformity test: CV = 21.5% at 6% addition, significantly better than 29.7% in the 0% group. Figure 10 The CV value for oil droplet distribution was lowest in the 6% group (21.5%).

[0097] Table 4 - Calculation of Coefficient of Variation

[0098] Mean particle size / pm Standard deviation (SD) / pm Coefficient of variation (CV) / % M01 18.30 5.43 29.7 M02 12.26 3.24 26.4 M03 11.67 2.51 21.5 M04 13.72 3.34 24.3 M05 18.75 5.14 27.4

[0099] b. Chemical property testing:

[0100] pH value measurement: When added at 6% and 8%, the pH value is 5.95-5.99, which is within the safe range of 4-6 for human skin.

[0101] Heavy metal testing: lead content 0.1708-0.7125μg / L, copper content 0.09947-2.025μg / L, both <1mg / kg, in accordance with national standard GB / T 35828-2018.

[0102] Table 5 - Determination of lead content in samples

[0103]

[0104]

[0105] Table 6 - Determination of Copper Content in Samples

[0106] M04 sampling concentration 0.09 pg / mL 1.2 pg / mL 1.5 pg / mL 1.8 pg / mL 2.1 pg / mL Copper content (ug / L) 0.09947 1.094 1.581 1.888 2.025 Absorbance (A) 0.010 0.104 0.150 0.179 0.192

[0107] c. Use performance testing:

[0108] Adhesion test: Adhesion is optimal at an addition rate of 6%.

[0109] Friction test: μ = 0.18 at 6% addition, indicating moderate friction.

[0110] Table 7 - Calculation Results of Friction Force Test

[0111] Sample M01 M02 M03 M04 M05 u value 0.44 0.20 0.18 0.26 0.62 Evaluation Reasonable Reasonable Reasonable Reasonable Reasonable Reasonable

[0112] d. Safety and stability testing:

[0113] Skin irritation test: No rash or discomfort.

[0114] Stability test: No significant difference, comparable to the stability of commercially available oral balm composition "R".

[0115] 3) Experimental Conclusions

[0116] Analysis of experimental data revealed that avocado extract at a concentration of 6% formed a stable network structure with the matrix, ensuring hardness and heat resistance while preventing excessive lipids from compatibility with the matrix. Furthermore, at a concentration of 6%, the active ingredients and oils synergistically improved uniformity and adhesion, verifying the non-linear correlation between extract concentration and performance indicators. The study also clarified standardized procedures for each test, providing a standard basis for industrial quality control. This invention systematically solves the problems in existing technologies and provides a scientific method for the development of oral balm compositions containing natural extracts.

[0117] This invention is the first to integrate extraction process optimization (yield ≥8%), formula gradient design (0-10% gradient addition), and 12 performance tests (physical / chemical / usage / safety) to achieve a systematic evaluation from raw materials to finished products. Through a nonlinear correlation model, the synergistic relationship between avocado extract concentration (5-7%) and matrix components is accurately analyzed. Experiments verify that at a 6% addition, hardness increases by 47% (250g vs 170g) and uniformity coefficient of variation decreases by 28% (CV = 21.5% vs 29.7%).

[0118] On the other hand, the present invention employs key test parameters such as the unified capillary method (heating rate 1℃ / min), cantilever beam method (cantilever 12.1mm), and ICH accelerated test to ensure that the data is comparable across platforms.

[0119] Furthermore, the method described in this invention supports flexible replacement of extraction solvents (ethanol / n-hexane), wax bases (carnauba wax / candelilla wax), and detection instruments (laser particle size analyzer / texture analyzer), and predicts the performance of compound components through models (such as DPPH removal rate increased to 90%).

[0120] This method provides a replicable scientific evaluation system for the development of natural lip balms, significantly shortening the R&D cycle and reducing the cost of empirical trial and error. Examples demonstrate that the lip balm composition optimized based on this system outperforms commercially available products in key indicators such as heat resistance (melting point 69℃), safety (lead content <0.7125μg / L), and user experience (adhesion 0.00247g, μ=0.18), showing potential for large-scale production.

Claims

1. A systematic evaluation method for lip balm compositions containing natural avocado extract, characterized in that, Includes the following steps: (1) Targeted extraction of active ingredients from avocado: Mix the pulp and extraction solvent at a mass ratio of 1:(1.8-2.2), reflux at 75-85℃ for 14-18 hours to obtain avocado extract, add 0.5-1.5% allantoin for preservation and store at 2-8℃. (2) Add 0-10% avocado extract to the fixation matrix, wherein the fixation matrix components include 6-10% beeswax, 15-20% paraffin, 6-10% coconut oil, 3-5% lake, 0.01-0.03% lemon oil, 0.8-1.2% vanilla extract, 4-6% monostearate, 1-3% titanium dioxide, 40-48% castor oil, 4-6% lanolin, and 8-12% isopropyl palmitate to form a comparative formulation; (3) Multi-dimensional performance tests include: melting point determination, hardness test, homogeneity test, uniformity test, and stability test; (4) Parameter correlation analysis: Establish a nonlinear correlation model to determine the optimal addition ratio of avocado extract; (5) Standardized testing.

2. The systematic evaluation method for lip balm compositions containing natural avocado extract according to claim 1, characterized in that: In step (1), the extraction solvent is ethyl acetate, ethanol or n-hexane, the extraction temperature is 65-90℃, and the extraction time is 10-24 hours.

3. The systematic evaluation method for lip balm compositions containing natural avocado extract according to claim 1, characterized in that: In step (2), the fixed matrix is ​​a wax matrix, carnauba wax or candelilla wax, with a melting point range of 55-90℃ and a hardness fracture load of 80-300g.

4. The systematic evaluation method for lip balm compositions containing natural avocado extract according to claim 1, characterized in that: The melting point was determined using the capillary method; the hardness was tested using a cantilever beam fracture test; the homogeneity test involved coating the lip balm composition onto a polydimethylsiloxane film and detecting the transmittance using an ultraviolet spectrophotometer; and the uniformity test involved observing the distribution of lip granules under a microscope and calculating the coefficient of variation.

5. The systematic evaluation method for lip balm compositions containing natural avocado extract according to claim 1, characterized in that: The homogeneity test uses a laser particle size analyzer or a colorimeter; the hardness test uses a weight loading method, and a texture analyzer can be selected in parallel.

6. The systematic evaluation method for lip balm compositions containing natural avocado extract according to claim 1, characterized in that: The colorimeter measures a color difference value ΔE ≤ 3.5 after illumination.

7. The systematic evaluation method for lip balm compositions containing natural avocado extract according to claim 6, characterized in that: The texture analyzer method has a trigger force of 5-15g and an accuracy deviation of ≤10g.

8. The systematic evaluation method for lip balm compositions containing natural avocado extract according to claim 1, characterized in that: The optimal addition ratio of avocado extract in step (4) is 5-7%, with corresponding performance indicators as follows: melting point 65-72℃, hardness 200-280g, uniformity coefficient of variation CV≤25%, and pH value 5.5-6.

2.

9. The systematic evaluation method for lip balm compositions containing natural avocado extract according to claim 1, characterized in that: The optimal addition ratio of avocado extract resulted in an adhesion strength of 0.001-0.004 g (measured by the tape adhesion method).

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