Angelica dahurica and oyster peptide beverage for preventing nodules and preparation method thereof

Abstract

The invention relates to the technical field of preparation of health-care beverages, and particularly discloses a nodule-preventing radix angelicae and oyster peptide beverage and a preparation method thereof, and the nodule-preventing radix angelicae and oyster peptide beverage comprises the following raw materials: oyster peptide powder, a compound traditional Chinese medicine extract, zero-calorie syrup, xanthan gum, citric acid and purified water. The preparation method of the radix angelicae and oyster peptide beverage comprises the following steps: mixing the oyster peptide powder, the compound traditional Chinese medicine extract and the purified water, uniformly stirring, adding the zero-calorie syrup, the xanthan gum and the citric acid, homogenizing, and sterilizing to obtain the radix angelicae and oyster peptide beverage for preventing nodules. The prepared radix angelicae and oyster peptide beverage is high in nodule inhibition rate, serum E2 and P can be accurately adjusted to be balanced to be close to the normal level, and the core inducement of hormone imbalance is effectively corrected. The composition can significantly reduce the content of inflammatory factors such as TNF-alpha and IL-6 in mammary tissue, improve the SOD activity and reduce the MDA level, and strongly inhibit inflammatory response and oxidative stress injury.

Description

Technical Field

[0001] This application relates to the field of health beverage preparation technology, and more specifically, it relates to an Angelica dahurica and oyster peptide beverage for preventing nodules and its preparation method. Background Technology

[0002] Nodular diseases (such as thyroid nodules and breast hyperplasia) have become a common health problem affecting modern people, and the demand for their prevention and treatment is increasing. In the search for solutions, the combination of traditional Chinese medicine, which is both food and medicine, and modern bioactive peptide technology has shown great potential.

[0003] Oysters, a traditional food and medicinal resource, have long been recorded in Traditional Chinese Medicine (TCM) as having the ability to "soften and disperse lumps," and are believed to be able to dissolve lumps in the body. Modern pharmacological research has further confirmed that oysters and their active ingredients possess various biological activities, including antioxidant, antitumor, and immunomodulatory effects. Oyster peptides, in particular, are small molecule fragments obtained through enzymatic hydrolysis of oyster protein, which are not only more easily absorbed by the human body but have also been found to have antitumor, antioxidant, and immunomodulatory effects. Studies show that these small molecule peptides can be directly absorbed in the intestines, at a rate faster than free amino acids and proteins. However, traditional oyster peptide preparation processes generally suffer from low yields of short peptides and complex ultrafiltration and purification processes after enzymatic hydrolysis, directly hindering their industrialization. Angelica dahurica, a traditional Chinese medicine, has the effects of dispelling wind and dampness, reducing swelling and draining pus, and is often used in TCM surgical prescriptions for nodule-related diseases. Combining Angelica dahurica with oysters to exert a synergistic effect provides a new approach to nodule prevention. Currently, some oyster peptide products have appeared on the market, but their quality varies, affecting market expansion. While there are reports on oyster peptide health drinks in the current technology, these products mostly focus on providing nutritional and health benefits, lacking specialized formulation designs for nodule prevention. Furthermore, these products suffer from significant shortcomings in taste, with strong fishy odors and poor palatability affecting consumer acceptance. In terms of preparation technology, traditional enzymatic hydrolysis techniques offer limited improvement in the yield of oyster short peptides, and subsequent separation and purification steps are cumbersome and costly. Therefore, developing a scientifically formulated oyster peptide drink that targets nodule prevention, has a pleasant taste, and utilizes advanced preparation technology not only meets market demand but also fully leverages the health benefits of oysters and angelica. Summary of the Invention

[0004] To address the technical problems mentioned in the background section, this application provides an Angelica dahurica and oyster peptide beverage for preventing nodules and its preparation method.

[0005] In the first aspect, this application provides an Angelica dahurica and oyster peptide beverage for preventing nodules, employing the following technical solution:

[0006] A angelica and oyster peptide beverage for preventing nodules comprises the following ingredients in parts by weight: 5-12 parts oyster peptide powder, 10-12 parts compound Chinese herbal extract, 5-10 parts zero-calorie syrup, 0.3-0.8 parts xanthan gum, 0.2-0.6 parts citric acid, and 100-120 parts purified water.

[0007] Preferably, the preparation method of the oyster peptide powder is as follows:

[0008] Step 1: Take fresh oysters, freeze them at -20℃ for 10-12 hours, homogenize them, add walnut protein, extraction aid and purified water, mix them and then sonicate for 20-30 minutes to obtain the enzymatic hydrolysis precursor;

[0009] Step 2: Add the compound enzyme preparation to the enzymatic hydrolysis precursor, and enzymatically hydrolyze at a constant temperature of 50-55℃ for 4-6 hours. Then, raise the temperature to 85-100℃ to inactivate the enzyme for 15-30 minutes. After cooling, add activated carbon for decolorization, filter, and dry to obtain oyster peptide powder.

[0010] Preferably, the extraction adjuvant in step 1 is composed of chitosan quaternary ammonium salt, soybean phosphatidylcholine and γ-polyglutamic acid in a mass ratio of 10-20:2-4:1-3; wherein the molecular weight of γ-polyglutamic acid is 50-100kDa.

[0011] Preferably, in step 1, the mass ratio of fresh oysters, walnut protein, extraction aid, and purified water is 30-40:10-15:5-10:100-120.

[0012] Preferably, the compound enzyme preparation in step 2 is composed of animal protein hydrolase, Bacillus subtilis protease and flavor protease in a mass ratio of 10-20:5-10:3-8.

[0013] Preferably, in step 2, the mass ratio of the enzymatic hydrolysis precursor to the complex enzyme preparation is 100-120:1-2.

[0014] Preferably, the preparation method of the compound traditional Chinese medicine extract is as follows:

[0015] (1) Ganoderma lucidum and Poria cocos are mixed, pulverized and sieved to obtain mushroom coarse powder; Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa and Prunus persica are mixed, pulverized and sieved to obtain herb coarse powder;

[0016] (2) Add the coarse powder of fungi to purified water and extract at a constant temperature of 90-95℃ for 2-3 times, each time for 2-4 hours. Combine the extracts to obtain fungi extract. Then add the coarse powder of herbs to purified water and reflux at 100-110℃ for 2-4 times, each time for 1-3 hours. Combine the extracts to obtain herbal extract.

[0017] (3) After mixing the mushroom extract and herbal extract evenly, a fermentation substrate is obtained. Fermentation bacteria are inoculated into the fermentation substrate, fermented for 30-40 hours, and dried at low temperature to obtain the compound Chinese medicine extract.

[0018] Preferably, in step (1), the mass ratio of Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa and Prunus persica is 1-2:1-3:2-4:5-8:2-6:2-6:1-3:1-3:1-2:1-2:1-3.

[0019] Preferably, the fermentation strain in step (3) consists of Bifidobacterium longum and Saccharomyces cerevisiae with an effective live bacteria ratio of 10-15:2-5.

[0020] Preferably, the inoculation amount of the fermentation strain in step (3) is 3-7% of the mass of the fermentation substrate.

[0021] Secondly, this application provides a method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules, using the following technical solution:

[0022] A method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules specifically includes the following preparation steps:

[0023] Mix oyster peptide powder, compound Chinese herbal extracts and purified water, stir well, add zero-calorie syrup, xanthan gum and citric acid, homogenize and sterilize to obtain Angelica dahurica oyster peptide drink for preventing nodules.

[0024] Preferably, the sterilization temperature is 120-135℃ and the sterilization time is 10-15s.

[0025] In summary, this application has the following beneficial effects:

[0026] This application enhances extraction efficiency through the synergistic effect of extraction aids in "solubilization-dispersion-protection": on the one hand, it reduces the interfacial tension between active ingredients and solvents, promoting the dissolution of oyster proteins; on the other hand, its components can form a protective film, reducing the oxidative degradation of active ingredients during ultrasonic and enzymatic hydrolysis. Simultaneously, the compounded extraction aids improve the system's dispersibility, preventing the aggregation of enzymatic precursors and ensuring a uniform and thorough enzymatic hydrolysis reaction. Compared to extraction with a single extraction aid, the dissolution rate and stability of active ingredients are significantly improved. The use of multiple proteases allows for synergistic hydrolysis of peptide bonds in different amino acid sequences of oyster and walnut proteins, broadening the substrate degradation range and avoiding the problems of incomplete hydrolysis and limited types of active peptide fragments associated with single enzymes. The compounded enzymes disrupt the protein's spatial structure through complementary interactions, significantly increasing the yield of oyster peptides while generating more small molecule peptide fragments with anti-inflammatory and hormone-regulating activities. This solves the defects of insufficient activity and limited function in single-enzyme hydrolysis products, laying the material foundation for the product's core efficacy.

[0027] The method employs a dual-strain synergistic fermentation combined with the extraction process of fungi and herbs, which has significant advantages over single-strain fermentation or mixed extraction: the dual strains complement each other metabolically, breaking down large molecular components (such as cellulose and pectin) in traditional Chinese medicine into easily absorbed small molecule active substances, and generating new metabolic products (such as short-chain fatty acids and secondary metabolites) to enhance anti-inflammatory and hormone regulation effects. The extraction process is adapted to the different physicochemical properties of fungi and herbs, maximizing the retention of their respective active ingredients and avoiding mutual interference between components during mixed extraction. Detailed Implementation

[0028] The present application will be further described in detail below with reference to the embodiments.

[0029] The walnut protein (content: 99%) used in the examples and comparative examples of this application was purchased from Hunan Xun'ao Bioengineering Co., Ltd.; chitosan quaternary ammonium salt was purchased from Xi'an Tianzheng Pharmaceutical Excipients Co., Ltd.; soybean phosphatidylcholine was purchased from Hubei Langbowan Biomedical Co., Ltd.; γ-polyglutamic acid (molecular weight 50-100kDa) was purchased from Shanghai Zhongfeng Biotechnology Co., Ltd.; animal protein hydrolase was purchased from Jiangxi Huihai Biotechnology Co., Ltd.; Bacillus subtilis protease (enzyme activity: 100,000 u / g) was purchased from Jiangxi Huihai Biotechnology Co., Ltd.; flavor protease (enzyme activity: 200,000 u / g) was purchased from Hebei Yulongxiang Biotechnology Co., Ltd.; Bifidobacterium longum (product number: B89493) and Saccharomyces cerevisiae (product number: B98029) were both purchased from Ningbo Mingzhou Biotechnology Co., Ltd.; and zero-calorie syrup was purchased from Dongguan Sentang Food Co., Ltd.

[0030] Examples 1-3 provide a Angelica dahurica and oyster peptide beverage for preventing nodules and its preparation method.

[0031] Example 1

[0032] A angelica and oyster peptide beverage for preventing nodules comprises the following ingredients in parts by weight: 5 parts oyster peptide powder, 10 parts compound Chinese herbal extract, 5 parts zero-calorie syrup, 0.3 parts xanthan gum, 0.2 parts citric acid, and 100 parts purified water.

[0033] The preparation method of oyster peptide powder is as follows:

[0034] Step 1: Take fresh oysters, freeze them at -20℃ for 10 hours, homogenize them, add walnut protein, extraction aid and purified water, mix them and sonicate for 20 minutes, control the ultrasonic power to be 100W and the ultrasonic frequency to be 40kHz to obtain the enzymatic hydrolysis precursor. The mass ratio of fresh oysters, walnut protein, extraction aid and purified water is 30:10:5:100. The extraction aid is composed of chitosan quaternary ammonium salt, soybean phosphatidylcholine and γ-polyglutamic acid in a mass ratio of 10:2:1.

[0035] Step 2: Add a compound enzyme preparation to the enzymatic hydrolysis precursor, controlling the mass ratio of the enzymatic hydrolysis precursor to the compound enzyme preparation to be 100:1. Perform enzymatic hydrolysis at 50℃ for 4 hours, then raise the temperature to 85℃ and inactivate the enzyme for 15 minutes. After cooling, add 1% of the mass of the enzymatic hydrolysis precursor with activated carbon for decolorization. After filtration, dry at 40℃ for 10 hours to obtain oyster peptide powder. The compound enzyme preparation consists of animal protein hydrolase, Bacillus subtilis protease and flavor protease in a mass ratio of 10:5:3.

[0036] The preparation method of the compound traditional Chinese medicine extract is as follows:

[0037] (1) Ganoderma lucidum and Poria cocos were mixed and pulverized through a 40-mesh sieve to obtain mushroom coarse powder; Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica were mixed and pulverized through a 40-mesh sieve to obtain herb coarse powder. The mass ratio of Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica was controlled to be 1:1:2:5:2:2:1:1:1:1:1;

[0038] (2) Add 10 times the amount of purified water to the coarse powder of fungi, extract twice at 90℃ for 2 hours each time, combine the extracts to obtain fungi extract; then add 8 times the amount of purified water to the coarse powder of herbs, reflux extract twice at 100℃ for 1 hour each time, combine the extracts to obtain herbal extract.

[0039] (3) Mix the mushroom extract and herbal extract at a stirring speed of 200 rpm for 20 min. After mixing evenly, obtain the fermentation substrate. Inoculate the fermentation substrate with fermentation bacteria, and control the inoculation amount of fermentation bacteria to 3% of the mass of the fermentation substrate. Perform anaerobic fermentation at a fermentation temperature of 30℃ and a fermentation pH of 5.0 for 30 h. Dry at a low temperature of 30℃ for 10 h to obtain the compound Chinese medicine extract. The fermentation bacteria consist of Bifidobacterium longum and Saccharomyces cerevisiae with an effective live bacteria ratio of 10:2.

[0040] A method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules includes the following preparation steps:

[0041] Mix oyster peptide powder, compound Chinese herbal extract and purified water, stir at 200 rpm for 30 minutes until uniform, then add zero-calorie syrup, xanthan gum and citric acid. Homogenize twice under high pressure at 25 MPa and 20℃ for 3 minutes each time. After homogenization, sterilize at 120℃ for 10 seconds to obtain the Angelica dahurica oyster peptide drink for preventing nodules.

[0042] Example 2

[0043] A angelica and oyster peptide beverage for preventing nodules comprises the following ingredients in parts by weight: 8 parts oyster peptide powder, 11 parts compound Chinese herbal extract, 8 parts zero-calorie syrup, 0.5 parts xanthan gum, 0.4 parts citric acid, and 110 parts purified water.

[0044] The preparation method of oyster peptide powder is as follows:

[0045] Step 1: Take fresh oysters, freeze them at -20℃ for 11 hours, homogenize them, add walnut protein, extraction aid and purified water, mix them and sonicate for 25 minutes, control the ultrasonic power at 110W and the ultrasonic frequency at 50kHz to obtain the enzymatic hydrolysis precursor. The mass ratio of fresh oysters, walnut protein, extraction aid and purified water is 35:12:8:110. The extraction aid is composed of chitosan quaternary ammonium salt, soybean phosphatidylcholine and γ-polyglutamic acid in a mass ratio of 15:3:2.

[0046] Step 2: Add a compound enzyme preparation to the enzymatic hydrolysis precursor, controlling the mass ratio of the enzymatic hydrolysis precursor to the compound enzyme preparation to be 110:1.5. Perform enzymatic hydrolysis at 52℃ for 5 hours, then raise the temperature to 92℃ and inactivate the enzyme for 22 minutes. After cooling, add 1.5% of the mass of the enzymatic hydrolysis precursor with activated carbon for decolorization. After filtration, dry at 50℃ for 15 hours to obtain oyster peptide powder. The compound enzyme preparation consists of animal protein hydrolase, Bacillus subtilis protease and flavor protease in a mass ratio of 15:8:5.

[0047] The preparation method of the compound traditional Chinese medicine extract is as follows:

[0048] (1) Ganoderma lucidum and Poria cocos were mixed and pulverized through a 50-mesh sieve to obtain mushroom coarse powder; Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica were mixed and pulverized through a 50-mesh sieve to obtain herb coarse powder. The mass ratio of Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica was controlled to be 1.5:2:3:6:4:4:2:2:1.5:1.5:2;

[0049] (2) Add 11 times the amount of purified water to the coarse powder of fungi, extract at 92℃ for 3 times, each time for 3 hours, combine the extracts to obtain fungi extract; then add 9 times the amount of purified water to the coarse powder of herbs, reflux at 105℃ for 3 times, each time for 2 hours, combine the extracts to obtain herbal extract.

[0050] (3) Mix the mushroom extract and herbal extract at a stirring speed of 300 rpm for 25 min. After mixing evenly, a fermentation substrate is obtained. Fermentation bacteria are inoculated into the fermentation substrate, and the inoculation amount of fermentation bacteria is controlled to be 5% of the mass of the fermentation substrate. The fermentation is carried out at a fermentation temperature of 32℃ and a fermentation pH of 5.5 for 35 h, and then dried at a low temperature of 35℃ for 15 h to obtain the compound Chinese medicine extract. The fermentation bacteria consist of Bifidobacterium longum and Saccharomyces cerevisiae with an effective live bacteria ratio of 12:3.

[0051] A method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules includes the following preparation steps:

[0052] Mix oyster peptide powder, compound Chinese herbal extract and purified water, stir at 300 rpm for 45 minutes until uniform, then add zero-calorie syrup, xanthan gum and citric acid. Homogenize under high pressure at 35 MPa and 28℃ for 58 minutes each time. After homogenization, sterilize at 128℃ for 12 seconds to obtain the Angelica dahurica oyster peptide drink for preventing nodules.

[0053] Example 3

[0054] A angelica and oyster peptide beverage for preventing nodules comprises the following ingredients in parts by weight: 12 parts oyster peptide powder, 12 parts compound Chinese herbal extract, 10 parts zero-calorie syrup, 0.8 parts xanthan gum, 0.6 parts citric acid, and 120 parts purified water.

[0055] The preparation method of oyster peptide powder is as follows:

[0056] Step 1: Take fresh oysters, freeze them at -20℃ for 10-12 hours, homogenize them, add walnut protein, extraction aid and purified water, mix them and sonicate for 30 minutes, control the ultrasonic power to be 120W and the ultrasonic frequency to be 60kHz to obtain the enzymatic hydrolysis precursor. The mass ratio of fresh oysters, walnut protein, extraction aid and purified water is 40:15:10:120. The extraction aid is composed of chitosan quaternary ammonium salt, soybean phosphatidylcholine and γ-polyglutamic acid in a mass ratio of 20:4:3.

[0057] Step 2: Add a compound enzyme preparation to the enzymatic hydrolysis precursor, controlling the mass ratio of the enzymatic hydrolysis precursor to the compound enzyme preparation to be 120:2. Perform enzymatic hydrolysis at 55℃ for 6 hours, then raise the temperature to 100℃ and inactivate the enzyme for 30 minutes. After cooling, add 2% of the mass of the enzymatic hydrolysis precursor with activated carbon for decolorization. After filtration, dry at 60℃ for 20 hours to obtain oyster peptide powder. The compound enzyme preparation consists of animal protein hydrolase, Bacillus subtilis protease and flavor protease in a mass ratio of 20:10:8.

[0058] The preparation method of the compound traditional Chinese medicine extract is as follows:

[0059] (1) Ganoderma lucidum and Poria cocos were mixed and pulverized through a 60-mesh sieve to obtain mushroom coarse powder; Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica were mixed and pulverized through a 60-mesh sieve to obtain herb coarse powder. The mass ratio of Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica was controlled to be 2:3:4:8:6:6:3:3:2:2:3;

[0060] (2) Add 12 times the amount of purified water to the coarse powder of fungi, extract at 95℃ for 3 times, 4 hours each time, combine the extracts to obtain fungi extract; then add 10 times the amount of purified water to the coarse powder of herbs, reflux at 110℃ for 4 times, 3 hours each time, combine the extracts to obtain herbal extract.

[0061] (3) Mix the mushroom extract and herbal extract at a stirring speed of 400 rpm for 30 min. After mixing evenly, a fermentation substrate is obtained. Fermentation bacteria are inoculated into the fermentation substrate, and the inoculation amount of fermentation bacteria is controlled to be 7% of the mass of the fermentation substrate. The fermentation is carried out at a fermentation temperature of 35℃ and a fermentation pH of 6.0 for 40 h, and then dried at a low temperature of 40℃ for 20 h to obtain the compound Chinese medicine extract. The fermentation bacteria consist of Bifidobacterium longum and Saccharomyces cerevisiae with an effective live bacteria ratio of 15:5.

[0062] A method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules includes the following preparation steps:

[0063] Mix oyster peptide powder, compound Chinese herbal extract and purified water, stir at 400 rpm for 60 minutes until uniform, then add zero-calorie syrup, xanthan gum and citric acid. Homogenize three times under high pressure at 40 MPa and 35℃ for 8 minutes each time. After homogenization, sterilize at 135℃ for 15 seconds to obtain the Angelica dahurica oyster peptide drink for preventing nodules.

[0064] Comparative Example 1

[0065] A angelica and oyster peptide beverage for preventing nodules comprises the following ingredients in parts by weight: 5 parts oyster peptide powder, 10 parts compound Chinese herbal extract, 5 parts zero-calorie syrup, 0.3 parts xanthan gum, 0.2 parts citric acid, and 100 parts purified water.

[0066] The preparation method of oyster peptide powder is as follows:

[0067] Step 1: Take fresh oysters, freeze them at -20℃ for 10 hours, homogenize them, add walnut protein, extraction aid and purified water, mix them and sonicate for 20 minutes, control the ultrasonic power to be 100W and the ultrasonic frequency to be 40kHz to obtain the enzymatic hydrolysis precursor. The mass ratio of fresh oysters, walnut protein, extraction aid and purified water is 30:10:5:100. The extraction aid is composed of chitosan quaternary ammonium salt and soybean phosphatidylcholine in a mass ratio of 10:3.

[0068] Step 2: Add a compound enzyme preparation to the enzymatic hydrolysis precursor, controlling the mass ratio of the enzymatic hydrolysis precursor to the compound enzyme preparation to be 100:1. Perform enzymatic hydrolysis at 50℃ for 4 hours, then raise the temperature to 85℃ and inactivate the enzyme for 15 minutes. After cooling, add 1% of the mass of the enzymatic hydrolysis precursor with activated carbon for decolorization. After filtration, dry at 40℃ for 10 hours to obtain oyster peptide powder. The compound enzyme preparation consists of animal protein hydrolase, Bacillus subtilis protease and flavor protease in a mass ratio of 10:5:3.

[0069] The preparation method of the compound traditional Chinese medicine extract is as follows:

[0070] (1) Ganoderma lucidum and Poria cocos were mixed and pulverized through a 40-mesh sieve to obtain mushroom coarse powder; Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica were mixed and pulverized through a 40-mesh sieve to obtain herb coarse powder. The mass ratio of Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica was controlled to be 1:1:2:5:2:2:1:1:1:1:1;

[0071] (2) Add 10 times the amount of purified water to the coarse powder of fungi, extract twice at 90℃ for 2 hours each time, combine the extracts to obtain fungi extract; then add 8 times the amount of purified water to the coarse powder of herbs, reflux extract twice at 100℃ for 1 hour each time, combine the extracts to obtain herbal extract.

[0072] (3) Mix the mushroom extract and herbal extract at a stirring speed of 200 rpm for 20 min. After mixing evenly, obtain the fermentation substrate. Inoculate the fermentation substrate with fermentation bacteria, and control the inoculation amount of fermentation bacteria to 3% of the mass of the fermentation substrate. Perform anaerobic fermentation at a fermentation temperature of 30℃ and a fermentation pH of 5.0 for 30 h. Dry at a low temperature of 30℃ for 10 h to obtain the compound Chinese medicine extract. The fermentation bacteria consist of Bifidobacterium longum and Saccharomyces cerevisiae with an effective live bacteria ratio of 10:2.

[0073] A method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules includes the following preparation steps:

[0074] Mix oyster peptide powder, compound Chinese herbal extract and purified water, stir at 200 rpm for 30 minutes until uniform, then add zero-calorie syrup, xanthan gum and citric acid. Homogenize twice under high pressure at 25 MPa and 20℃ for 3 minutes each time. After homogenization, sterilize at 120℃ for 10 seconds to obtain the Angelica dahurica oyster peptide drink for preventing nodules.

[0075] Comparative Example 2

[0076] A angelica and oyster peptide beverage for preventing nodules comprises the following ingredients in parts by weight: 5 parts oyster peptide powder, 10 parts compound Chinese herbal extract, 5 parts zero-calorie syrup, 0.3 parts xanthan gum, 0.2 parts citric acid, and 100 parts purified water.

[0077] The preparation method of oyster peptide powder is as follows:

[0078] Step 1: Take fresh oysters, freeze them at -20℃ for 10 hours, homogenize them, add walnut protein, extraction aid and purified water, mix them and sonicate for 20 minutes, control the ultrasonic power to be 100W and the ultrasonic frequency to be 40kHz to obtain the enzymatic hydrolysis precursor. The mass ratio of fresh oysters, walnut protein, extraction aid and purified water is 30:10:5:100. The extraction aid is composed of chitosan quaternary ammonium salt, soybean phosphatidylcholine and γ-polyglutamic acid in a mass ratio of 10:2:1.

[0079] Step 2: Add a compound enzyme preparation to the enzymatic hydrolysis precursor, controlling the mass ratio of the enzymatic hydrolysis precursor to the compound enzyme preparation to be 100:1. Perform enzymatic hydrolysis at 50℃ for 4 hours, then raise the temperature to 85℃ and inactivate the enzyme for 15 minutes. After cooling, add 1% of the mass of the enzymatic hydrolysis precursor with activated carbon for decolorization. After filtration, dry at 40℃ for 10 hours to obtain oyster peptide powder. The compound enzyme preparation consists of Bacillus subtilis protease and flavor protease in a mass ratio of 5:3.

[0080] The preparation method of the compound traditional Chinese medicine extract is as follows:

[0081] (1) Ganoderma lucidum and Poria cocos were mixed and pulverized through a 40-mesh sieve to obtain mushroom coarse powder; Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica were mixed and pulverized through a 40-mesh sieve to obtain herb coarse powder. The mass ratio of Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica was controlled to be 1:1:2:5:2:2:1:1:1:1:1;

[0082] (2) Add 10 times the amount of purified water to the coarse powder of fungi, extract twice at 90℃ for 2 hours each time, combine the extracts to obtain fungi extract; then add 8 times the amount of purified water to the coarse powder of herbs, reflux extract twice at 100℃ for 1 hour each time, combine the extracts to obtain herbal extract.

[0083] (3) Mix the mushroom extract and herbal extract at a stirring speed of 200 rpm for 20 min. After mixing evenly, obtain the fermentation substrate. Inoculate the fermentation substrate with fermentation bacteria, and control the inoculation amount of fermentation bacteria to 3% of the mass of the fermentation substrate. Perform anaerobic fermentation at a fermentation temperature of 30℃ and a fermentation pH of 5.0 for 30 h. Dry at a low temperature of 30℃ for 10 h to obtain the compound Chinese medicine extract. The fermentation bacteria consist of Bifidobacterium longum and Saccharomyces cerevisiae with an effective live bacteria ratio of 10:2.

[0084] A method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules includes the following preparation steps:

[0085] Mix oyster peptide powder, compound Chinese herbal extract and purified water, stir at 200 rpm for 30 minutes until uniform, then add zero-calorie syrup, xanthan gum and citric acid. Homogenize twice under high pressure at 25 MPa and 20℃ for 3 minutes each time. After homogenization, sterilize at 120℃ for 10 seconds to obtain the Angelica dahurica oyster peptide drink for preventing nodules.

[0086] Comparative Example 3

[0087] A angelica and oyster peptide beverage for preventing nodules comprises the following ingredients in parts by weight: 5 parts oyster peptide powder, 10 parts compound Chinese herbal extract, 5 parts zero-calorie syrup, 0.3 parts xanthan gum, 0.2 parts citric acid, and 100 parts purified water.

[0088] The preparation method of oyster peptide powder is as follows:

[0089] Step 1: Take fresh oysters, freeze them at -20℃ for 10 hours, homogenize them, add walnut protein, extraction aid and purified water, mix them and sonicate for 20 minutes, control the ultrasonic power to be 100W and the ultrasonic frequency to be 40kHz to obtain the enzymatic hydrolysis precursor. The mass ratio of fresh oysters, walnut protein, extraction aid and purified water is 30:10:5:100. The extraction aid is composed of chitosan quaternary ammonium salt, soybean phosphatidylcholine and γ-polyglutamic acid in a mass ratio of 10:2:1.

[0090] Step 2: Add a compound enzyme preparation to the enzymatic hydrolysis precursor, controlling the mass ratio of the enzymatic hydrolysis precursor to the compound enzyme preparation to be 100:1. Perform enzymatic hydrolysis at 50℃ for 4 hours, then raise the temperature to 85℃ and inactivate the enzyme for 15 minutes. After cooling, add 1% of the mass of the enzymatic hydrolysis precursor with activated carbon for decolorization. After filtration, dry at 40℃ for 10 hours to obtain oyster peptide powder. The compound enzyme preparation consists of animal protein hydrolysate and flavor protease in a mass ratio of 10:8.

[0091] The preparation method of the compound traditional Chinese medicine extract is as follows:

[0092] (1) Ganoderma lucidum and Poria cocos were mixed and pulverized through a 40-mesh sieve to obtain mushroom coarse powder; Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica were mixed and pulverized through a 40-mesh sieve to obtain herb coarse powder. The mass ratio of Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica was controlled to be 1:1:2:5:2:2:1:1:1:1:1;

[0093] (2) Add 10 times the amount of purified water to the coarse powder of fungi, extract twice at 90℃ for 2 hours each time, combine the extracts to obtain fungi extract; then add 8 times the amount of purified water to the coarse powder of herbs, reflux extract twice at 100℃ for 1 hour each time, combine the extracts to obtain herbal extract.

[0094] (3) Mix the mushroom extract and herbal extract at a stirring speed of 200 rpm for 20 min. After mixing evenly, obtain the fermentation substrate. Inoculate the fermentation substrate with fermentation bacteria, and control the inoculation amount of fermentation bacteria to 3% of the mass of the fermentation substrate. Perform anaerobic fermentation at a fermentation temperature of 30℃ and a fermentation pH of 5.0 for 30 h. Dry at a low temperature of 30℃ for 10 h to obtain the compound Chinese medicine extract. The fermentation bacteria consist of Bifidobacterium longum and Saccharomyces cerevisiae with an effective live bacteria ratio of 10:2.

[0095] A method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules includes the following preparation steps:

[0096] Mix oyster peptide powder, compound Chinese herbal extract and purified water, stir at 200 rpm for 30 minutes until uniform, then add zero-calorie syrup, xanthan gum and citric acid. Homogenize twice under high pressure at 25 MPa and 20℃ for 3 minutes each time. After homogenization, sterilize at 120℃ for 10 seconds to obtain the Angelica dahurica oyster peptide drink for preventing nodules.

[0097] Comparative Example 4

[0098] A angelica and oyster peptide beverage for preventing nodules comprises the following ingredients in parts by weight: 5 parts oyster peptide powder, 10 parts compound Chinese herbal extract, 5 parts zero-calorie syrup, 0.3 parts xanthan gum, 0.2 parts citric acid, and 100 parts purified water.

[0099] The preparation method of oyster peptide powder is as follows:

[0100] Step 1: Take fresh oysters, freeze them at -20℃ for 10 hours, homogenize them, add walnut protein, extraction aid and purified water, mix them and sonicate for 20 minutes, control the ultrasonic power to be 100W and the ultrasonic frequency to be 40kHz to obtain the enzymatic hydrolysis precursor. The mass ratio of fresh oysters, walnut protein, extraction aid and purified water is 30:10:5:100. The extraction aid is composed of chitosan quaternary ammonium salt, soybean phosphatidylcholine and γ-polyglutamic acid in a mass ratio of 10:2:1.

[0101] Step 2: Add a compound enzyme preparation to the enzymatic hydrolysis precursor, controlling the mass ratio of the enzymatic hydrolysis precursor to the compound enzyme preparation to be 100:1. Perform enzymatic hydrolysis at 50℃ for 4 hours, then raise the temperature to 85℃ and inactivate the enzyme for 15 minutes. After cooling, add 1% of the mass of the enzymatic hydrolysis precursor with activated carbon for decolorization. After filtration, dry at 40℃ for 10 hours to obtain oyster peptide powder. The compound enzyme preparation consists of animal protein hydrolase and Bacillus subtilis protease in a mass ratio of 10:8.

[0102] The preparation method of the compound traditional Chinese medicine extract is as follows:

[0103] (1) Ganoderma lucidum and Poria cocos were mixed and pulverized through a 40-mesh sieve to obtain mushroom coarse powder; Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica were mixed and pulverized through a 40-mesh sieve to obtain herb coarse powder. The mass ratio of Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica was controlled to be 1:1:2:5:2:2:1:1:1:1:1;

[0104] (2) Add 10 times the amount of purified water to the coarse powder of fungi, extract twice at 90℃ for 2 hours each time, combine the extracts to obtain fungi extract; then add 8 times the amount of purified water to the coarse powder of herbs, reflux extract twice at 100℃ for 1 hour each time, combine the extracts to obtain herbal extract.

[0105] (3) Mix the mushroom extract and herbal extract at a stirring speed of 200 rpm for 20 min. After mixing evenly, obtain the fermentation substrate. Inoculate the fermentation substrate with fermentation bacteria, and control the inoculation amount of fermentation bacteria to 3% of the mass of the fermentation substrate. Perform anaerobic fermentation at a fermentation temperature of 30℃ and a fermentation pH of 5.0 for 30 h. Dry at a low temperature of 30℃ for 10 h to obtain the compound Chinese medicine extract. The fermentation bacteria consist of Bifidobacterium longum and Saccharomyces cerevisiae with an effective live bacteria ratio of 10:2.

[0106] A method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules includes the following preparation steps:

[0107] Mix oyster peptide powder, compound Chinese herbal extract and purified water, stir at 200 rpm for 30 minutes until uniform, then add zero-calorie syrup, xanthan gum and citric acid. Homogenize twice under high pressure at 25 MPa and 20℃ for 3 minutes each time. After homogenization, sterilize at 120℃ for 10 seconds to obtain the Angelica dahurica oyster peptide drink for preventing nodules.

[0108] Comparative Example 5

[0109] A angelica and oyster peptide beverage for preventing nodules comprises the following ingredients in parts by weight: 5 parts oyster peptide powder, 10 parts compound Chinese herbal extract, 5 parts zero-calorie syrup, 0.3 parts xanthan gum, 0.2 parts citric acid, and 100 parts purified water.

[0110] The preparation method of oyster peptide powder is as follows:

[0111] Step 1: Take fresh oysters, freeze them at -20℃ for 10 hours, homogenize them, add walnut protein, extraction aid and purified water, mix them and sonicate for 20 minutes, control the ultrasonic power to be 100W and the ultrasonic frequency to be 40kHz to obtain the enzymatic hydrolysis precursor. The mass ratio of fresh oysters, walnut protein, extraction aid and purified water is 30:10:5:100. The extraction aid is composed of chitosan quaternary ammonium salt, soybean phosphatidylcholine and γ-polyglutamic acid in a mass ratio of 10:2:1.

[0112] Step 2: Add a compound enzyme preparation to the enzymatic hydrolysis precursor, controlling the mass ratio of the enzymatic hydrolysis precursor to the compound enzyme preparation to be 100:1. Perform enzymatic hydrolysis at 50℃ for 4 hours, then raise the temperature to 85℃ and inactivate the enzyme for 15 minutes. After cooling, add 1% of the mass of the enzymatic hydrolysis precursor with activated carbon for decolorization. After filtration, dry at 40℃ for 10 hours to obtain oyster peptide powder. The compound enzyme preparation consists of animal protein hydrolase, Bacillus subtilis protease and flavor protease in a mass ratio of 10:5:3.

[0113] The preparation method of the compound traditional Chinese medicine extract is as follows:

[0114] (1) Ganoderma lucidum and Poria cocos were mixed and pulverized through a 40-mesh sieve to obtain mushroom coarse powder; Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica were mixed and pulverized through a 40-mesh sieve to obtain herb coarse powder. The mass ratio of Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica was controlled to be 1:1:2:5:2:2:1:1:1:1:1;

[0115] (2) Add 10 times the amount of purified water to the coarse powder of fungi, extract twice at 90℃ for 2 hours each time, combine the extracts to obtain fungi extract; then add 8 times the amount of purified water to the coarse powder of herbs, reflux extract twice at 100℃ for 1 hour each time, combine the extracts to obtain herbal extract.

[0116] (3) Mix the mushroom extract and herbal extract at a stirring speed of 200 rpm for 20 min. After mixing evenly, a fermentation substrate is obtained. Fermentation bacteria are inoculated into the fermentation substrate, and the inoculation amount of fermentation bacteria is controlled to be 3% of the mass of the fermentation substrate. The fermentation is carried out at a fermentation temperature of 30℃ and a fermentation pH of 5.0 for 30 h, followed by low-temperature drying at 30℃ for 10 h to obtain the compound Chinese medicine extract. The fermentation bacteria is brewing yeast.

[0117] A method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules includes the following preparation steps:

[0118] Mix oyster peptide powder, compound Chinese herbal extract and purified water, stir at 200 rpm for 30 minutes until uniform, then add zero-calorie syrup, xanthan gum and citric acid. Homogenize twice under high pressure at 25 MPa and 20℃ for 3 minutes each time. After homogenization, sterilize at 120℃ for 10 seconds to obtain the Angelica dahurica oyster peptide drink for preventing nodules.

[0119] Comparative Example 6

[0120] A angelica and oyster peptide beverage for preventing nodules comprises the following ingredients in parts by weight: 5 parts oyster peptide powder, 10 parts compound Chinese herbal extract, 5 parts zero-calorie syrup, 0.3 parts xanthan gum, 0.2 parts citric acid, and 100 parts purified water.

[0121] The preparation method of oyster peptide powder is as follows:

[0122] Step 1: Take fresh oysters, freeze them at -20℃ for 10 hours, homogenize them, add walnut protein, extraction aid and purified water, mix them and sonicate for 20 minutes, control the ultrasonic power to be 100W and the ultrasonic frequency to be 40kHz to obtain the enzymatic hydrolysis precursor. The mass ratio of fresh oysters, walnut protein, extraction aid and purified water is 30:10:5:100. The extraction aid is composed of chitosan quaternary ammonium salt, soybean phosphatidylcholine and γ-polyglutamic acid in a mass ratio of 10:2:1.

[0123] Step 2: Add a compound enzyme preparation to the enzymatic hydrolysis precursor, controlling the mass ratio of the enzymatic hydrolysis precursor to the compound enzyme preparation to be 100:1. Perform enzymatic hydrolysis at 50℃ for 4 hours, then raise the temperature to 85℃ and inactivate the enzyme for 15 minutes. After cooling, add 1% of the mass of the enzymatic hydrolysis precursor with activated carbon for decolorization. After filtration, dry at 40℃ for 10 hours to obtain oyster peptide powder. The compound enzyme preparation consists of animal protein hydrolase, Bacillus subtilis protease and flavor protease in a mass ratio of 10:5:3.

[0124] The preparation method of the compound traditional Chinese medicine extract is as follows:

[0125] (1) Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica are mixed, pulverized, and passed through a 40-mesh sieve to obtain mixed powder; the mass ratio of Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa, and Prunus persica is controlled to be 1:1:2:5:2:2:1:1:1:1:1;

[0126] (2) Add 10 times the amount of purified water to the mixed powder, extract twice at 90℃ for 2 hours each time, combine the extracts to obtain the fermentation substrate;

[0127] (3) Inoculate the fermentation substrate with fermentation strains, and control the inoculation amount of fermentation strains to 3% of the mass of fermentation substrate. At a fermentation temperature of 30℃ and a fermentation pH of 5.0, perform anaerobic fermentation for 30h, and dry at a low temperature of 30℃ for 10h to obtain compound Chinese medicine extract. The fermentation strains consist of Bifidobacterium longum and Saccharomyces cerevisiae with an effective live bacteria ratio of 10:2.

[0128] A method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules includes the following preparation steps:

[0129] Mix oyster peptide powder, compound Chinese herbal extract and purified water, stir at 200 rpm for 30 minutes until uniform, then add zero-calorie syrup, xanthan gum and citric acid. Homogenize twice under high pressure at 25 MPa and 20℃ for 3 minutes each time. After homogenization, sterilize at 120℃ for 10 seconds to obtain the Angelica dahurica oyster peptide drink for preventing nodules.

[0130] Performance testing

[0131] Based on the core inducing factor of "estrogen-progesterone imbalance" in clinical breast hyperplasia / nodules, a rat breast nodule model was constructed by exogenous injection of estrogen and progesterone. The breast nodule prevention effects of the Angelica dahurica and oyster peptide drinks prepared in Examples 1-3 and Comparative Examples 1-6 of this application were compared and evaluated to clarify the synergistic advantages of the product formula and process.

[0132] Animal modeling

[0133] SPF-grade SD female nonpregnant rats, weighing 200-220g, were selected. The experiment was conducted after 7 days of acclimatization feeding, with a temperature of 22-25℃, relative humidity of 50%-60%, 12-hour light-dark cycle, and free access to food and water.

[0134] Diethylstilbestrol (purity ≥98%, Shanghai Yuanye Biotechnology Co., Ltd.); progesterone (purity ≥99%, Sigma-Aldrich); rat estrogen (E2) and progesterone (P) ELISA kits; rat TNF-α, IL-6, and IL-1β ELISA kits; superoxide dismutase (SOD) activity assay kits and malondialdehyde (MDA) content assay kits were all purchased from Nanjing Jiancheng Bioengineering Institute.

[0135] Operating procedures:

[0136] Rats that had undergone adaptive feeding were randomly divided into 11 groups of 10 rats each. The specific groupings are as follows: Blank control group; Model control group; Example 1 group; Example 2 group; Example 3 group; Comparative example 1 group; Comparative example 2 group; Comparative example 3 group; Comparative example 4 group; Comparative example 5 group; Comparative example 6 group. The modeling period lasted 4 weeks, using a two-step method of "estrogen pretreatment + progesterone induction." The specific procedure is as follows:

[0137] Modeling procedure: The blank control group was injected with an equal volume of physiological saline throughout the entire process; the model control group and all sample groups (Examples 1-3 and Comparative Examples 1-6) were injected with diethylstilbestrol (0.5 mg / kg body weight, diluted with physiological saline to 0.1 mL / 100 g) intraperitoneally daily from day 1 to day 14, and progesterone (5 mg / kg body weight, diluted with physiological saline to 0.1 mL / 100 g) intraperitoneally daily from day 15 to day 28.

[0138] Administration procedure: On the first day of modeling, the drugs were administered to the groups simultaneously. The blank control group and the model control group were given an equal volume of physiological saline (20 mL / kg body weight) by gavage daily. The groups of Examples 1-3 and Comparative Examples 1-6 were given the corresponding samples by gavage daily (dose 20 mL / kg body weight). The gavage time was fixed from 9:00 to 10:00 am every day for 28 consecutive days.

[0139] On day 28 of modeling, rats were anesthetized intraperitoneally with 10% chloral hydrate (3 mL / kg body weight), and blood was collected from the abdominal aorta. After sacrifice, the second pair of mammary gland tissue from both sides was dissected (standard site for detecting mammary nodules in rats). The total weight of the mammary gland tissue was accurately weighed using an electronic balance. The number of nodules was observed and counted visually and under a dissecting microscope (nodules with a diameter ≥0.5 mm were defined as detectable). The maximum diameter (d) of each nodule was measured using calipers, and the volume of a single nodule was calculated using the formula V=πd³ / 6. The average value of each group was taken. The nodule inhibition rate was calculated using the following formula:

[0140] Nodule inhibition rate (%) = (average nodule weight in the model control group - average nodule weight in the sample group) / average nodule weight in the model control group × 100%;

[0141] Serum hormone level detection: After blood was collected from the abdominal aorta, the blood was centrifuged at 3000 r / min for 15 min (4℃) to separate the serum. The concentrations of E2 and P in the serum were calculated using an ELISA kit.

[0142] Detection of inflammatory factors in breast tissue: Take 0.5g of breast tissue, add 4.5mL of physiological saline, prepare 10% tissue homogenate using a tissue homogenizer, centrifuge at 3000r / min for 15min (4℃), take the supernatant, and detect the content of inflammatory factors TNF-α, IL-6 and IL-1β using an ELISA kit;

[0143] Detection of oxidative stress indicators in breast tissue: Take 10% of the above-mentioned breast tissue homogenate and use the SOD activity detection kit (xanthine oxidase method) and MDA content detection kit (thiobarbituric acid method) to detect SOD activity and MDA content respectively.

[0144] The specific results are shown in Table 1.

[0145] Table 1. The preventive effect of Angelica dahurica and oyster peptide drinks prepared in Examples 1-3 and Comparative Examples 1-6 on nodules (x±s, n=10)

[0146] Table 2. The preventive effect of Angelica dahurica and oyster peptide drinks prepared in Examples 1-3 and Comparative Examples 1-6 on nodules (x±s, n=10)

[0147] As shown in Tables 1 and 2, the Angelica dahurica and oyster peptide beverage prepared in this application exhibits a nodule inhibition rate of up to 97.8%, precisely regulating serum E2 and P balance to near-normal levels and effectively correcting the core cause of hormonal imbalance. It can significantly reduce the content of inflammatory factors such as TNF-α and IL-6 in breast tissue, increase SOD activity, and reduce MDA levels, effectively inhibiting inflammatory responses and oxidative stress damage.

[0148] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A angelica and oyster peptide beverage for preventing nodules, characterized in that, The ingredients include the following parts by weight: 5-12 parts oyster peptide powder, 10-12 parts compound Chinese herbal extract, 5-10 parts zero-calorie syrup, 0.3-0.8 parts xanthan gum, 0.2-0.6 parts citric acid, and 100-120 parts purified water.

2. The Angelica dahurica and oyster peptide beverage for preventing nodules according to claim 1, characterized in that, The preparation method of the oyster peptide powder is as follows: Step 1: Take fresh oysters, freeze them at -20℃ for 10-12 hours, homogenize them, add walnut protein, extraction aid and purified water, mix them and then sonicate for 20-30 minutes to obtain the enzymatic hydrolysis precursor; Step 2: Add the compound enzyme preparation to the enzymatic hydrolysis precursor, and enzymatically hydrolyze at a constant temperature of 50-55℃ for 4-6 hours. Then, raise the temperature to 85-100℃ to inactivate the enzyme for 15-30 minutes. After cooling, add activated carbon for decolorization, filter, and dry to obtain oyster peptide powder.

3. The Angelica dahurica and oyster peptide beverage for preventing nodules according to claim 2, characterized in that, The auxiliary agent extracted in step 1 is composed of chitosan quaternary ammonium salt, soybean phosphatidylcholine and γ-polyglutamic acid in a mass ratio of 10-20:2-4:1-3; wherein, the molecular weight of γ-polyglutamic acid is 50-100kDa.

4. The Angelica dahurica and oyster peptide beverage for preventing nodules according to claim 2, characterized in that, In step 1, the mass ratio of fresh oysters, walnut protein, extraction aid, and purified water is 30-40:10-15:5-10:100-120.

5. The Angelica dahurica and oyster peptide beverage for preventing nodules according to claim 2, characterized in that, In step 2, the compound enzyme preparation consists of animal protein hydrolase, Bacillus subtilis protease, and flavor protease in a mass ratio of 10-20:5-10:3-8.

6. The Angelica dahurica and oyster peptide beverage for preventing nodules according to claim 2, characterized in that, In step 2, the mass ratio of the enzymatic hydrolysis precursor to the complex enzyme preparation is 100-120:1-2.

7. The Angelica dahurica and oyster peptide beverage for preventing nodules according to claim 1, characterized in that, The preparation method of the compound traditional Chinese medicine extract is as follows: (1) Ganoderma lucidum and Poria cocos are mixed, pulverized and sieved to obtain mushroom coarse powder; Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa and Prunus persica are mixed, pulverized and sieved to obtain herb coarse powder; (2) Add the coarse powder of fungi to purified water and extract at a constant temperature of 90-95℃ for 2-3 times, each time for 2-4 hours. Combine the extracts to obtain fungi extract. Then add the coarse powder of herbs to purified water and reflux at 100-110℃ for 2-4 times, each time for 1-3 hours. Combine the extracts to obtain herbal extract. (3) After mixing the mushroom extract and herbal extract evenly, a fermentation substrate is obtained. Fermentation bacteria are inoculated into the fermentation substrate, fermented for 30-40 hours, and dried at low temperature to obtain the compound Chinese medicine extract.

8. The Angelica dahurica and oyster peptide beverage for preventing nodules according to claim 7, characterized in that, In step (1), the mass ratio of Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica dahurica, Platycodon grandiflorus, Prunella vulgaris, Taraxacum mongolicum, Laminaria japonica, Sinapis alba, Curcuma longa and Prunus persica is 1-2:1-3:2-4:5-8:2-6:2-6:1-3:1-3:1-2:1-2:1-3.

9. The Angelica dahurica and oyster peptide beverage for preventing nodules according to claim 7, characterized in that, In step (3), the fermentation strain consists of Bifidobacterium longum and Saccharomyces cerevisiae with an effective live bacteria ratio of 10-15:2-5; the inoculation amount of the fermentation strain is 3-7% of the mass of the fermentation substrate.

10. A method for preparing an Angelica dahurica and oyster peptide beverage for preventing nodules according to any one of claims 1-9, characterized in that, Specifically, the preparation steps include the following: Mix oyster peptide powder, compound Chinese herbal extracts and purified water, stir well, add zero-calorie syrup, xanthan gum and citric acid, homogenize and sterilize to obtain Angelica dahurica oyster peptide drink for preventing nodules.

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