Plant extract nourishing makeup removing wet tissue and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses a plant extract nourishing makeup removal wet tissue and a preparation method thereof, the plant extract nourishing makeup removal wet tissue comprises the following raw materials by weight: 15-20 parts of an efficient cleaning component, 2-3 parts of a water locking and moisturizing component, 4-8 parts of a plant extract active component, 0.08-0.16 part of a stabilizer, and 84-92 parts of deionized water; the invention starts from two aspects: firstly, the essential oil, the mandelic acid and the glycyrrhizin are compounded; through the synergistic effect of the three components, the trouble that traditional cleansing oil needs to be emulsified is avoided, and the problem that common cleansing water is poor in cleansing power, so that cleaning is not thorough or skin is damaged due to repeated wiping is solved; 2, compounding a cactus extracting solution, an olive leaf extract, a soapberry bark extract and a synergistic surfactant, and performing enzymolysis and fermentation to obtain a plant extraction active component; through the synergistic effect of the multiple components, the dry and tight feeling after makeup removal is reduced, skin irritation is relieved, and meanwhile the antibacterial performance of the makeup removal wet tissue is improved.

Description

Technical Field

[0001] This invention relates to the field of cosmetics technology, and more specifically, to a plant extract nourishing makeup remover wipe and its preparation method. Background Technology

[0002] Makeup remover wipes are moisturizing products made from non-woven fabric with an added amount of makeup remover, primarily used for quickly removing makeup. With rising consumer demand, their role in daily cleansing and skincare is becoming increasingly prominent. High-quality makeup remover wipes not only possess strong cleansing power but also often offer additional soothing and skincare benefits to meet consumers' demands for multifunctional and high-quality makeup removal products.

[0003] Makeup remover is the core ingredient in makeup remover wipes for cleansing and disinfection. However, to maintain antibacterial or bacteriostatic effects, some products add chemical bactericides, which may irritate the skin and pose a risk of sensitization with long-term use. In recent years, the use of plant extracts as natural alternatives has become a trend, such as using natural oils and extracts with soothing properties. However, these active ingredients are easily affected by the environment, presenting technical challenges such as generally poor makeup removal and antibacterial properties.

[0004] Therefore, there is an urgent need in the market for a plant-based nourishing makeup remover wipe that can effectively remove heavy makeup, is non-irritating to the skin, and has excellent antibacterial properties, in order to meet consumers' demand for efficient, gentle, and convenient makeup removal products.

[0005] Based on the above statements, the present invention provides a plant-based nourishing makeup remover wipe and a method for preparing the same. Summary of the Invention

[0006] To address the problems mentioned in the background section, this invention provides a plant-based nourishing makeup remover wipe and its preparation method.

[0007] A plant-based nourishing makeup remover wipe, comprising a wipe base and makeup-removing active ingredients impregnated on the wipe base; Preferably, the makeup-removing active ingredient comprises the following raw materials in parts by weight: 15-20 parts of high-efficiency cleansing component, 2-3 parts of water-locking and moisturizing component, 4-8 parts of plant-derived active ingredient, 0.08-0.16 parts of stabilizer, and 84-92 parts of deionized water.

[0008] A method for preparing plant extract nourishing makeup remover wipes includes the following preparation steps: Step S1: Add the water-locking and moisturizing components to deionized water, heat to 40-45℃, stir evenly to obtain the aqueous phase; Step S2: Add the high-efficiency cleaning component to the aqueous system, heat to 72-74℃, and stir for 24-36 minutes to obtain the composite emulsion phase; Step S3: Add the stabilizer to the composite emulsion phase, continue stirring for 18-22 minutes, cool to 35-40℃, add the plant extract active ingredients, stir evenly, adjust the pH to 5.5-6.5, stir for 8-12 minutes to obtain the makeup removal active ingredients. Step S4: Impregnate the wet wipe substrate with the makeup remover active ingredients, and control the mass ratio of the makeup remover active ingredients to the wet wipe substrate to be 2-6:1 to obtain plant extract nourishing makeup remover wipes.

[0009] Preferably, the highly effective cleaning component is a mixture of essential oils, glycyrrhizin and mandelic acid in a mass ratio of 5-12:2-5:1-3.

[0010] Preferably, the essential oil is at least one of lemon essential oil, Atlantic cedar essential oil, and cypress essential oil.

[0011] Preferably, the water-locking and moisturizing component is at least one of D-panthenol, hyaluronic acid, and γ-polyglutamic acid.

[0012] Preferably, the stabilizer is at least one of carbomer and hydroxyethyl cellulose.

[0013] Preferably, the wet wipe substrate is a blend of corn fiber and Tencel fabric spun at a mass ratio of 2-4:1-3, with a basis weight of 45-55 g / m². 2 .

[0014] Preferably, the method for preparing the plant extract active ingredient includes the following preparation steps: Step A1: Wash and crush the cactus, add deionized water at a material-to-liquid ratio of 1:12-16, soak, filter, and obtain cactus extract. Then add olive leaf extract, soap bark extract, synergistic surfactant, and deionized water, mix evenly, add cellulase for enzymatic hydrolysis, obtain compound enzymatic hydrolysate, sterilize, inoculate with Aspergillus niger for primary fermentation, obtain compound fermentation broth, adjust the pH of compound fermentation broth, inoculate with compound bacteria, carry out secondary fermentation, sterilize at low temperature, and obtain plant fermentation product. The mass ratio of cactus extract, olive leaf extract, soap bark extract, synergistic surfactant, and deionized water is 10-16:2.2-3:0.6-0.8:0.2-0.4:50. Step A2: Mix the plant ferment, purslane extract and jojoba seed oil evenly to obtain the plant extract active components, wherein the mass ratio of plant ferment, purslane extract and jojoba seed oil is 9-13:2.6-3.4:1.2-1.6.

[0015] Preferably, in step A1, the amount of cellulase added is 0.16-0.24% of the dry weight of the cactus.

[0016] Preferably, in step A1, the cellulase activity is 60,000-160,000 U / g.

[0017] Preferably, during the enzymatic hydrolysis process in step A1, the pH is 6.8-7.2, the temperature is 48-52℃, and the hydrolysis time is 1.8-2.2h.

[0018] Preferably, in step A1, the inoculum amount of Aspergillus niger is 2.4-2.8% of the mass of the compound enzyme hydrolysate.

[0019] Preferably, in step A2, the inoculation amount of the compound microbial strain is 5.6-6.4% of the mass of the compound fermentation broth.

[0020] Preferably, in step A2, the compound microbial strain is composed of Aspergillus niger and Trichoderma reesei with an effective viable count ratio of 2-3:1.

[0021] Preferably, the synergistic surfactant is prepared by the following steps: Chitosan was dissolved in an aqueous acetic acid solution and stirred until homogeneous. The solution was then filtered through a microporous membrane to obtain a chitosan-acetic acid solution. A mixture of octenyl succinic anhydride and anhydrous ethanol (a) was then added dropwise. After the addition was complete, the temperature was raised to 72-76℃ and the reaction was stirred for 4.2-5.4 hours. The pH was then adjusted to 6.4-6.8. The solution was filtered, washed, and dried to obtain a synergistic surfactant. The mass ratio of chitosan, aqueous acetic acid solution, and mixture a was 1:18-24:60-80. The mass ratio of octenyl succinic anhydride to anhydrous ethanol in mixture a was 1-2:60. In the above process, the hydroxyl groups on the surface of chitosan reacted with octenyl succinic anhydride to undergo a ring-opening esterification reaction, resulting in the grafting of molecular chains containing terminal carboxyl groups and unsaturated double bonds onto the surface of chitosan.

[0022] Preferably, the acetic acid aqueous solution has a mass fraction of 2-4%.

[0023] Preferably, the pore size of the microporous filter membrane is 0.2-0.4 μm.

[0024] Compared with the prior art, the present invention has the following beneficial effects: This invention utilizes a combination of lipophilic essential oils, amphiphilic mandelic acid, and emulsifying glycyrrhizin. These three components work synergistically. The essential oils and mandelic acid, with their excellent lipophilic properties, penetrate and break down solid / semi-solid oily makeup and dirt on the skin's surface, transforming them into a flowable liquid state, preventing makeup residue from clogging pores. Glycyrrhizin, as a natural surfactant, not only encapsulates the broken-down oil droplets to form water-soluble micelles but also exerts its own excellent anti-inflammatory and repairing effects, preventing skin friction damage from repeated wiping while accelerating skin barrier repair and reducing moisture loss after makeup removal. Mandelic acid slowly penetrates the skin, gently exfoliating dead skin cells, avoiding the irritation of traditional salicylic acid or glycolic acid. Through the synergistic effect of these three components, the product avoids the need for emulsification required by traditional cleansing oils and overcomes the problems of weak cleansing power in ordinary cleansing waters, leading to incomplete cleansing or requiring repeated wiping and skin damage. It simultaneously provides the product with the dual benefits of acid-level cleansing and soothing skincare, achieving a balance between high efficiency and gentleness.

[0025] This invention utilizes a compound of cactus extract, olive leaf extract, soap bark extract, and synergistic surfactants, followed by enzymatic hydrolysis and fermentation to obtain plant-derived active components. The cactus extract contains active ingredients such as malonic acid and quercetin-3-glucoside, which, through enzymatic fermentation, are converted into smaller molecules, improving sugar metabolism, reducing the release of inflammatory factors, and enhancing the soothing effect. Olive leaf extract contains oleuropein, which can inhibit LPS-induced inflammatory factor secretion, and hydroxytyrosol has excellent DPPH free radical scavenging ability; both can also prevent skin sensitivity after makeup removal. Soap bark extract is an ideal natural surfactant that can replace... Chemical emulsifiers reduce skin irritation from makeup remover wipes. Additionally, soap bark extract contains tannins, which have antibacterial properties and can alleviate skin redness and swelling caused by friction from the wipes. The synergistic surfactant is prepared by chemically bonding chitosan and octenyl succinic anhydride. This synergistic surfactant has a flexible long-chain alkane structure and a chitosan structure, providing excellent emulsification. Furthermore, the small molecules such as chitosan oligosaccharides obtained after enzymatic hydrolysis and fermentation possess excellent moisturizing, soothing, and antibacterial properties. Through the synergistic effect of these multiple components, the dryness and tightness after makeup removal are reduced, skin irritation is alleviated, and antibacterial properties are improved. Detailed Implementation

[0026] To make the embodiments of the present invention easier to understand, the present invention will be described in detail below with reference to specific embodiments. These embodiments are for illustrative purposes only and are not limited to the application scope of the present invention.

[0027] The present invention will be further described in detail below with reference to the embodiments.

[0028] The anhydrous ethanol used in the embodiments and comparative examples of this invention is food-grade anhydrous ethanol; lemon essential oil was purchased from Ji'an Huatianbao Traditional Chinese Medicine Biological Products Factory, CAS number 8008-56-8; Atlantic cedarwood essential oil was purchased from Ji'an Huatianbao Traditional Chinese Medicine Biological Products Factory, CAS number 92201-55-3; cypress essential oil was purchased from Ji'an Huatianbao Traditional Chinese Medicine Biological Products Factory, CAS number 8013-86-3; D-panthenol was purchased from Shanghai Quanyan Biotechnology Co., Ltd., CAS number 81-13-0; hyaluronic acid was purchased from Jining Tangyi Chemical Co., Ltd., CAS number 2013-6-25; γ-polyglutamic acid was purchased from Sichuan Huanxu Biotechnology Co., Ltd.; carbomer was purchased from Shaanxi Tangyao Biotechnology Co., Ltd., CAS number 9007-20-9; hydroxyethyl cellulose was purchased from Shandong Xuchen Chemical Technology Co., Ltd. The following products were purchased: Model QP-4400H; Olive leaf extract (CAS No. 149183-75-5) from Hubei Sanxin Biotechnology Co., Ltd.; Glycyrrhizin (CAS No. 1405-86-3) from Hubei Kemaidi Chemical Co., Ltd.; Soapberry bark extract (Catalog No. HH1250OVDOOH) from Shaanxi Shengqi Biotechnology Co., Ltd.; Portulaca oleracea extract from Guangzhou Huayu Biotechnology Co., Ltd.; Jojoba seed oil from Hubei Xingdongcheng Chemical Co., Ltd. (CAS No. 61789-91-1); Cellulase from Hubei Shineng Chemical Technology Co., Ltd. (CAS No. 9012-54-8); Aspergillus niger from Guangdong Fangxin Biotechnology Co., Ltd. (Product No. PC91877); and Trichoderma reesei from Wuhan Lanabai Pharmaceutical Chemical Co., Ltd. (CAS No. 9012-54-8). Chitosan was purchased from Wuhan Kanos Technology Co., Ltd., CAS No. 9012-76-4; mandelic acid was purchased from Shandong Liansheng Chemical Co., Ltd., product number JSY0500; citrate-phosphate buffer was commercially available from Shanghai Yuanye Biotechnology Co., Ltd., pH 6.8, model R27026; all other raw materials not mentioned were commercially available.

[0029] Preparation Examples 1-3 and Comparative Preparation Example 1 provide an enhanced surfactant.

[0030] Preparation Example 1 This preparation example provides a synergistic surfactant, which is prepared by the following steps: Chitosan was dissolved in a 2% (w / w) aqueous acetic acid solution and stirred at 600 rpm for 22 min until homogeneous. The solution was then filtered through a 0.2 μm polyethersulfone microporous membrane to obtain a chitosan-acetic acid solution. A mixture of octenyl succinic anhydride and anhydrous ethanol (a) was then added dropwise over 10 min at a rate of 3 drops / second. After the addition was complete, the temperature was raised to 72 °C and the reaction was stirred for 5.4 h. The pH was then adjusted to 6.4 with a 16% (w / w) sodium carbonate aqueous solution. The solution was filtered and washed three times each with anhydrous ethanol and deionized water. The solution was dried at 55 °C to constant weight to obtain a synergistic surfactant. The mass ratio of chitosan, aqueous acetic acid solution, and mixture a was 1:18:60, and the mass ratio of octenyl succinic anhydride to anhydrous ethanol in mixture a was 1:60.

[0031] Preparation Example 2 This preparation example provides a synergistic surfactant, which is prepared by the following steps: Chitosan was dissolved in a 3% (w / w) aqueous acetic acid solution and stirred at 650 rpm for 18 min until homogeneous. The solution was then filtered through a 0.3 μm polyethersulfone microporous membrane to obtain a chitosan-acetic acid solution. A mixture of octenyl succinic anhydride and anhydrous ethanol (a) was then added dropwise over 10 min at a rate of 4 drops / second. After the addition was complete, the temperature was raised to 74 °C, and the stirring was continued for 4.8 h while maintaining the stirring speed. The pH was then adjusted to 6.6 with an 18% (w / w) sodium carbonate aqueous solution. The solution was filtered and washed four times each with anhydrous ethanol and deionized water. The solution was then dried at 60 °C to constant weight to obtain a synergistic surfactant. The mass ratio of chitosan, aqueous acetic acid solution, and mixture a was 1:21:70, and the mass ratio of octenyl succinic anhydride to anhydrous ethanol in mixture a was 1.5:60.

[0032] Preparation Example 3 This preparation example provides a synergistic surfactant, which is prepared by the following steps: Chitosan was dissolved in a 4% (w / w) aqueous acetic acid solution and stirred at 700 rpm for 14 min until homogeneous. The solution was then filtered through a 0.4 μm polyethersulfone microporous membrane to obtain a chitosan-acetic acid solution. A mixture of octenyl succinic anhydride and anhydrous ethanol (a) was then added dropwise over 10 min at a rate of 5 drops / second. After the addition was complete, the temperature was raised to 76 °C, and the stirring speed was maintained for 4.2 h. The pH was then adjusted to 6.8 with a 20% (w / w) sodium carbonate aqueous solution. The solution was filtered and washed five times each with anhydrous ethanol and deionized water. The solution was dried at 65 °C to constant weight to obtain a synergistic surfactant. The mass ratio of chitosan, aqueous acetic acid solution, and mixture a was 1:24:80, and the mass ratio of octenyl succinic anhydride to anhydrous ethanol in mixture a was 2:60.

[0033] Comparative Preparation Example 1 This comparative preparation example provides a synergistic surfactant, which is prepared by the following steps: Chitosan was dissolved in a 2% (w / w) aqueous acetic acid solution and stirred at 600 rpm for 22 min until homogeneous. The solution was then filtered through a 0.2 μm polyethersulfone microporous membrane to obtain a chitosan-acetic acid solution. A mixture of citric anhydride and anhydrous ethanol (a) was then added dropwise over 10 min at a rate of 3 drops / second. After the addition was complete, the temperature was raised to 72 °C and the reaction was stirred for 5.4 h. The pH was then adjusted to 6.4 with a 16% (w / w) sodium carbonate aqueous solution. The solution was filtered and washed three times each with anhydrous ethanol and deionized water. The solution was then dried at 55 °C to constant weight to obtain a synergistic surfactant. The mass ratio of chitosan, aqueous acetic acid solution, and mixture a was 1:18:60, and the mass ratio of citric anhydride to anhydrous ethanol in mixture a was 1:60.

[0034] Preparation Examples 4-6 and Comparative Preparation Examples 2-4 provide a method for preparing plant extract active components.

[0035] Preparation Example 4 This preparation example provides a method for preparing a plant extract active ingredient, including the following preparation steps: Step A1: Wash and crush the cactus, soak it for 8 hours at a material-to-liquid ratio of 1:12, filter to obtain cactus extract, then add olive leaf extract, soap bark extract, the synergistic surfactant prepared in Example 1, and deionized water. Stir at 600 rpm for 30 minutes until well mixed, add cellulase with an enzyme activity of 60000 U / g for enzymatic hydrolysis to obtain a compound enzymatic hydrolysate, sterilize, and inoculate with Aspergillus niger at an inoculation rate of 2.4% of the compound enzymatic hydrolysate. Ferment at 28℃ for 68 hours to obtain a compound fermentation broth. The pH of the compound fermentation broth was adjusted to 5.0 using a citrate-phosphate buffer solution of 6.8. A compound microbial culture was inoculated at 5.6% of the broth's mass. Secondary fermentation was carried out at 30℃. During fermentation, the aeration rate was controlled at 0.9 vvm, the stirring speed at 220 rpm, and the fermentation time was 24 h. After fermentation, the mixture was sterilized at 56℃ for 28 min to obtain the plant fermentation product. The mass ratio of cactus extract, olive leaf extract, soap bark extract, synergistic surfactant, and deionized water was 10:2.2:0.6:0.2:50. The amount of cellulase added was 0.16% of the cactus dry weight. The specific enzymatic hydrolysis conditions were: pH 6.8, hydrolysis temperature 48℃, and hydrolysis time 2.2 h. The compound microbial culture consisted of a mixture of Aspergillus niger and Trichoderma reesei with an effective viable count ratio of 2:1. Step A2: Mix the plant ferment, purslane extract and jojoba seed oil at 3000 rpm for 16 minutes until homogeneous to obtain the plant extract active components. The mass ratio of plant ferment, purslane extract and jojoba seed oil is 9:2.6:1.2.

[0036] Preparation Example 5 This preparation example provides a method for preparing a plant extract active ingredient, including the following preparation steps: Step A1: Wash and crush the cactus, add deionized water at a material-to-liquid ratio of 1:14, soak for 10 hours, filter to obtain cactus extract, then add olive leaf extract, soap bark extract, the synergistic surfactant prepared in Example 2, and deionized water, stir at 650 rpm for 26 minutes until uniformly mixed, add cellulase with an enzyme activity of 110,000 U / g for enzymatic hydrolysis to obtain a compound enzymatic hydrolysate, sterilize, inoculate with Aspergillus niger at an inoculation amount of 2.6% of the compound enzymatic hydrolysate, and carry out a single fermentation at 30℃ for 60 hours to obtain a compound fermentation broth, which is then prepared using pH... The pH of the compound fermentation broth was adjusted to 5.2 using a citrate-phosphate buffer solution of 6.8. The compound microbial strain was inoculated at 6% of the broth's mass. Secondary fermentation was carried out at 31℃. During fermentation, the aeration rate was controlled at 1 vvm, the stirring speed at 260 rpm, and the fermentation time was 28 h. After fermentation, the mixture was sterilized at 58℃ for 25 min to obtain the plant fermentation product. The mass ratio of cactus extract, olive leaf extract, soap bark extract, synergistic surfactant, and deionized water was 13:2.6:0.7:0.3:50. The amount of cellulase added was 0.2% of the cactus dry weight. The specific enzymatic hydrolysis conditions were: pH 7, temperature 50℃, and time 2 h. The compound microbial strain consisted of a mixture of Aspergillus niger and Trichoderma reesei with an effective viable count ratio of 2.5:1. Step A2: Mix the plant ferment, purslane extract and jojoba seed oil at 3500 rpm for 14 minutes until homogeneous to obtain the plant extract active components. The mass ratio of plant ferment, purslane extract and jojoba seed oil is 11:3:1.4.

[0037] Preparation Example 6 This preparation example provides a method for preparing a plant extract active ingredient, including the following preparation steps: Step A1: Wash and crush the cactus, add deionized water at a material-to-liquid ratio of 1:16, soak for 12 hours, filter to obtain cactus extract, then add olive leaf extract, soap bark extract, the synergistic surfactant prepared in Example 3, and deionized water, stir at 700 rpm for 22 minutes until uniformly mixed, add cellulase with an enzyme activity of 160,000 U / g for enzymatic hydrolysis to obtain a compound enzymatic hydrolysate, sterilize, inoculate with Aspergillus niger at an inoculation amount of 2.8% of the compound enzymatic hydrolysate, and carry out a single fermentation at 32℃ for 60 hours to obtain a compound fermentation broth, which is then prepared using pH... The pH of the compound fermentation broth was adjusted to 5.5 using a citrate-phosphate buffer solution of 6.8. A compound microbial culture was inoculated at 6.4% of the broth's mass. Secondary fermentation was carried out at 32℃. During fermentation, the aeration rate was controlled at 1.1 vvm, the stirring speed at 300 rpm, and the fermentation time was 30 h. After fermentation, the mixture was sterilized at 60℃ for 22 min to obtain the plant fermentation product. The mass ratio of cactus extract, olive leaf extract, soap bark extract, synergistic surfactant, and deionized water was 16:3:0.8:0.4:50. The amount of cellulase added was 0.24% of the cactus dry weight. The specific enzymatic hydrolysis conditions were: pH 7.2, hydrolysis temperature 52℃, and hydrolysis time 1.8 h. The compound microbial culture consisted of a mixture of Aspergillus niger and Trichoderma reesei with an effective viable cell ratio of 3:1. Step A2: Mix the plant ferment, purslane extract and jojoba seed oil at 4000 rpm for 12 minutes until homogeneous to obtain the plant extract active components. The mass ratio of plant ferment, purslane extract and jojoba seed oil is 13:3.4:1.6.

[0038] Comparative Preparation Example 2 This comparative preparation example provides a method for preparing a plant extract active ingredient, including the following preparation steps: Step A1: Wash and crush the cactus, add deionized water at a material-to-liquid ratio of 1:12, soak for 8 hours, filter to obtain cactus extract, then add olive leaf extract, soap bark extract, the synergistic surfactant prepared in Comparative Preparation Example 1, and deionized water, stir at 600 rpm for 30 minutes until uniformly mixed, add cellulase with an enzyme activity of 60000 U / g for enzymatic hydrolysis to obtain a compound enzymatic hydrolysate, sterilize, inoculate with Aspergillus niger at an inoculation amount of 2.4% of the compound enzymatic hydrolysate, and carry out a single fermentation at 28℃ for 68 hours to obtain a compound fermentation broth, and use pH... The pH of the compound fermentation broth was adjusted to 5.0 using a citrate-phosphate buffer solution of 6.8. A compound microbial culture was inoculated at 5.6% of the broth's mass. Secondary fermentation was carried out at 30℃. During fermentation, the aeration rate was controlled at 0.9 vvm, the stirring speed at 220 rpm, and the fermentation time was 24 h. After fermentation, the mixture was sterilized at 56℃ for 28 min to obtain the plant fermentation product. The mass ratio of cactus extract, olive leaf extract, soap bark extract, synergistic surfactant, and deionized water was 10:2.2:0.6:0.2:50. The amount of cellulase added was 0.16% of the cactus dry weight. The specific enzymatic hydrolysis conditions were: pH 6.8, temperature 48℃, and hydrolysis time 2.2 h. The compound microbial culture consisted of a mixture of *Aspergillus niger* and *Trichoderma reesei* with an effective viable cell ratio of 2:1. Step A2: Mix the plant ferment, purslane extract and jojoba seed oil at 3000 rpm for 16 minutes until homogeneous to obtain the plant extract active components. The mass ratio of plant ferment, purslane extract and jojoba seed oil is 9:2.6:1.2.

[0039] Comparative preparation example 3 This comparative preparation example provides a method for preparing a plant extract active ingredient, including the following preparation steps: Step A1: Wash and crush the cactus, add deionized water at a material-to-liquid ratio of 1:12, soak for 8 hours, filter to obtain cactus extract, then add olive leaf extract, the synergistic surfactant prepared in Example 1, and deionized water, stir at 600 rpm for 30 minutes until uniformly mixed, add cellulase with an enzyme activity of 60000 U / g for enzymatic hydrolysis to obtain a compound enzymatic hydrolysate, sterilize, inoculate with Aspergillus niger at an inoculation amount of 2.4% of the compound enzymatic hydrolysate, and carry out a single fermentation at 28℃ for 68 hours to obtain a compound fermentation broth, and use pH... The pH of the compound fermentation broth was adjusted to 5.0 using a citrate-phosphate buffer solution of 6.8. A compound microbial culture was inoculated at 5.6% of the broth's mass. Secondary fermentation was carried out at 30℃. During fermentation, the aeration rate was controlled at 0.9 vvm, the stirring speed at 220 rpm, and the fermentation time was 24 h. After fermentation, the mixture was sterilized at 56℃ for 28 min to obtain the plant fermentation product. The mass ratio of cactus extract, olive leaf extract, synergistic surfactant, and deionized water was 10:2.8:0.2:50. The amount of cellulase added was 0.16% of the dry weight of the cactus. Specific enzymatic hydrolysis conditions were: pH controlled to 6.8 using a phosphate buffer solution of pH 7.4, temperature at 48℃, and hydrolysis time at 2.2 h. The compound microbial culture consisted of a mixture of *Aspergillus niger* and *Trichoderma reesei* with an effective viable cell ratio of 2:1. Step A2: Mix the plant ferment, purslane extract and jojoba seed oil at 3000 rpm for 16 minutes until homogeneous to obtain the plant extract active components. The mass ratio of plant ferment, purslane extract and jojoba seed oil is 9:2.6:1.2.

[0040] Comparative preparation example 4 This preparation example provides a method for preparing a plant extract active ingredient, including the following preparation steps: Step A1: Wash and crush the cactus, add deionized water at a material-to-liquid ratio of 1:12, soak for 8 hours, filter to obtain cactus extract, then add soap bark extract, the synergistic surfactant prepared in Example 1, and deionized water, stir at 600 rpm for 30 minutes until uniformly mixed, add cellulase with an enzyme activity of 60000 U / g for enzymatic hydrolysis to obtain a compound enzymatic hydrolysate, sterilize, inoculate with Aspergillus niger at an inoculation amount of 2.4% of the compound enzymatic hydrolysate, and carry out a single fermentation at 28℃ for 68 hours to obtain a compound fermentation broth, which is then prepared using pH... The pH of the compound fermentation broth was adjusted to 5.0 using a citrate-phosphate buffer solution of 6.8. A compound microbial culture was inoculated at 5.6% of the broth's mass. Secondary fermentation was carried out at 30℃. During fermentation, the aeration rate was controlled at 0.9 vvm, the stirring speed at 220 rpm, and the fermentation time was 24 h. After fermentation, the mixture was sterilized at 56℃ for 28 min to obtain the plant fermentation product. The mass ratio of cactus extract, soap bark extract, synergistic surfactant, and deionized water was 10:2.8:0.2:50. The amount of cellulase added was 0.16% of the dry weight of the cactus. The specific enzymatic hydrolysis conditions were: pH controlled to 6.8 using a phosphate buffer solution of pH 7.4, a temperature of 48℃, and a hydrolysis time of 2.2 h. The compound microbial culture consisted of a mixture of *Aspergillus niger* and *Trichoderma reesei* with an effective viable cell ratio of 2:1. Step A2: Mix the plant ferment, purslane extract and jojoba seed oil at 3000 rpm for 16 minutes until homogeneous to obtain the plant extract active components. The mass ratio of plant ferment, purslane extract and jojoba seed oil is 9:2.6:1.2.

[0041] Example 1 This embodiment provides a plant-based nourishing makeup remover wipe, comprising a wipe base material and makeup remover active ingredients impregnated on the wipe base material; the makeup remover active ingredients comprise the following raw materials in parts by weight: 15 parts of high-efficiency cleansing component, 2 parts of D-panthenol, 4 parts of the plant-based active ingredient prepared in Preparation Example 4, 0.08 parts of carbomer, and 84 parts of deionized water; the wipe base material is a blend of corn fiber and Tencel fabric in a mass ratio of 2:1, with a basis weight of 45 g / m². 2 ; The preparation method of this plant-based nourishing makeup remover wipe includes the following steps: Step S1: Add D-panthenol to deionized water, heat to 40°C, and stir at 300 rpm for 12 minutes until homogeneous to obtain an aqueous phase; Step S2: Add the high-efficiency cleaning component to the aqueous phase system, heat to 72°C, control the rotation speed at 1600 rpm, and stir for 36 min to obtain a composite emulsion phase. The high-efficiency cleaning component is a mixture of lemon essential oil, glycyrrhizin and mandelic acid in a mass ratio of 5:2:1. Step S3: Add carbomer to the composite emulsion phase, control the speed at 800 rpm, continue stirring for 22 min, cool down to 35℃, add the plant extract active ingredients, control the speed at 30 rpm and stir for 36 min until uniform, adjust the pH of the system to 5.5 with 0.5% Tris-HCl buffer, stir for 8 min to obtain the makeup removal active ingredients; Step S4: Impregnate the wet wipe substrate with the makeup remover active ingredients, and control the mass ratio of the makeup remover active ingredients to the wet wipe substrate to be 2:1 to obtain plant extract nourishing makeup remover wipes.

[0042] Example 2 This embodiment provides a plant-based nourishing makeup remover wipe, comprising a wipe base material and makeup remover active ingredients impregnated on the wipe base material; the makeup remover active ingredients comprise the following raw materials in parts by weight: 17.5 parts of high-efficiency cleansing component, 2.5 parts of hyaluronic acid, 6 parts of plant-based active ingredients prepared in Preparation Example 5, 0.12 parts of hydroxyethyl cellulose, and 88 parts of deionized water; the wipe base material is a blend of corn fiber and Tencel fabric in a mass ratio of 3:2, with a basis weight of 50 g / m². 2 ; The preparation method of this plant-based nourishing makeup remover wipe includes the following steps: Step S1: Add hyaluronic acid to deionized water, heat to 42°C, and stir at 350 rpm for 10 minutes until homogeneous to obtain an aqueous phase; Step S2: Add the high-efficiency cleaning component to the aqueous phase system, heat to 73°C, and stir at 1800 rpm for 30 minutes until homogeneous to obtain a composite emulsion phase. The high-efficiency cleaning component is composed of Atlantic cedarwood essential oil, glycyrrhizin and mandelic acid in a mass ratio of 8.5:3.5:2. Step S3: Add hydroxyethyl cellulose to the composite emulsion phase, control the speed at 850 rpm, continue stirring for 20 min, cool down to 37℃, add the plant extract active ingredients, control the speed at 45 rpm and stir for 32 min until uniform, adjust the pH of the system to 6 with 1% Tris-HCl buffer, stir for 10 min to obtain the makeup removal active ingredients. Step S4: Impregnate the wet wipe substrate with the makeup remover active ingredients, and control the mass ratio of the makeup remover active ingredients to the wet wipe substrate to be 4:1 to obtain plant extract nourishing makeup remover wipes.

[0043] Example 3 This embodiment provides a plant-based nourishing makeup remover wipe, comprising a wipe base material and makeup remover active ingredients impregnated on the wipe base material; the makeup remover active ingredients comprise the following raw materials in parts by weight: 20 parts of high-efficiency cleansing component, 3 parts of γ-polyglutamic acid, 8 parts of plant-based active ingredients prepared in Preparation Example 6, 0.16 parts of carbomer, and 92 parts of deionized water; the wipe base material is a blend of corn fiber and Tencel fabric in a mass ratio of 4:3, with a basis weight of 55 g / m². 2 ; The preparation method of this plant-based nourishing makeup remover wipe includes the following steps: Step S1: Add γ-polyglutamic acid to deionized water, heat to 45°C, and stir at 400 rpm for 8 minutes until homogeneous to obtain an aqueous phase; Step S2: Add the high-efficiency cleaning component to the aqueous phase system, heat to 74°C, and stir at 2000 rpm for 24 minutes to obtain a composite emulsion phase. The high-efficiency cleaning component is a mixture of cypress essential oil, glycyrrhizin and mandelic acid in a mass ratio of 12:5:3. Step S3: Add carbomer to the composite emulsion phase, control the speed at 900 rpm, continue stirring for 18 min, cool down to 35℃, add the plant extract active ingredients, control the speed at 60 rpm and stir for 28 min until uniform, adjust the pH of the system to 6.5 with 1.5% Tris-HCl buffer, stir for 12 min to obtain the makeup removal active ingredients; Step S4: Impregnate the wet wipe substrate with the makeup remover active ingredients, and control the mass ratio of the makeup remover active ingredients to the wet wipe substrate to be 6:1 to obtain plant extract nourishing makeup remover wipes.

[0044] Comparative Example 1 Comparative Example 1 is the same as Example 1, except that the plant extract active component in Example 1 is replaced with the plant extract active component prepared in Comparative Preparation Example 2.

[0045] Comparative Example 2 Comparative Example 2 is the same as Example 1, except that the plant extract active component in Example 1 is replaced with the plant extract active component prepared in Comparative Preparation Example 3.

[0046] Comparative Example 3 Comparative Example 3 is the same as Example 1, except that the plant extract active component in Example 1 is replaced with the plant extract active component prepared in Comparative Preparation Example 4.

[0047] Comparative Example 4 Comparative Example 4 is the same as Example 1, except that the mandelic acid in Example 1 is replaced with an equal mass of glycyrrhizin.

[0048] Comparative Example 5 Comparative Example 5 is the same as Example 1, except that the glycyrrhizin in Example 1 is replaced with an equal mass of mandelic acid.

[0049] Performance testing I. Skin Irritation Effect Test: 160 participants meeting the subject criteria were randomly divided into 8 groups of 20 each. Skin irritation tests were conducted on the plant-based nourishing makeup remover wipes provided in Examples 1-3 and Comparative Examples 1-5. The active makeup remover components from the test subjects were placed into the small chambers of the patch applicator, with a dosage of approximately 0.020-0.03 mL. No substance was placed in the control well. The patch applicator containing the test substance was applied to the back or flexor side of the forearm of the subject using hypoallergenic adhesive tape, and the palm was gently pressed to ensure even application to the skin for 24 hours. Skin reactions were observed 30 minutes and 24 hours after the patch applicator was removed, and the results were recorded. The grading criteria are shown in Table 1, and the test results are shown in Table 2. Table 1. Grading Criteria for Skin Reaction in Skin Patch Testing Table 2. Skin irritation test results of plant-based nourishing makeup remover wipes prepared in Examples 1-3 and Comparative Examples 1-5. As shown in Table 2, compared with Comparative Examples 1-5, the plant-based nourishing makeup remover wipes prepared in Examples 1-3 are non-irritating to the skin and have significant advantages.

[0050] II. Makeup Removal and Cleansing Power Test: 160 participants meeting the subject criteria were randomly divided into 8 groups of 20 each. The plant-based nourishing makeup remover wipes provided in Examples 1-3 and Comparative Examples 1-5 were tested. Commercially available makeup products (including foundation, eyeshadow, lipstick, and sunscreen) were applied to the forearm (1.25 mg / cm²). After 0.5 hours, the plant-based nourishing makeup remover wipes provided in Examples 1-3 and Comparative Examples 1-5 were applied to the makeup-covered area. Each subject used 0.4 g of the wipes and wiped for 25 seconds, then rinsed off with water. Skin brightness (L) values ​​of the test areas were measured before, after, and after makeup removal to calculate the makeup removal and cleansing ability (S-value). The average value was calculated using the formula shown below. The test results are shown in Table 3. Where S = makeup removal and cleansing ability, and L = brightness value after washing.

[0051] Table 3. Makeup removal and cleansing ability tests of the plant-based nourishing makeup remover wipes prepared in Examples 1-3 and Comparative Examples 1-5. As shown in Table 3, compared with Comparative Examples 1-5, the plant extract nourishing makeup remover wipes prepared in Examples 1-3 have a better makeup removal effect.

[0052] III. Antibacterial effect test: Referring to GB 15979-2024, the antibacterial properties of the plant-based nourishing makeup remover wipes prepared in Examples 1-3 and Comparative Examples 1-5 were tested. 5 mL of the sample extract and 5 mL of the control extract were placed in test tubes, and 100 mL of the prepared bacterial suspension was added to each. The mixture was stirred thoroughly and the timer was started. After 2 minutes, 0.5 mL of the solution was added to a test tube containing 5 mL of PBS solution, stirred thoroughly, and diluted appropriately. Then, 0.5 mL of each of the 2-3 dilutions was taken and placed in two separate petri dishes. The samples were incubated on inverted plates at 40-45°C to count the average colony count (B) of the sample and the average colony count (A) of the control. The antibacterial rates against *Escherichia coli*, *Staphylococcus aureus*, and *Candida albicans* were tested using the same method. Another set of samples was placed in a constant temperature and humidity environment of 37±1℃ and 70%RH for 12 weeks. Then, the inhibition rate of the samples against Escherichia coli, Staphylococcus aureus, and Candida albicans was tested. The inhibition rate after 2 minutes of bacterial treatment was recorded as the inhibition rate. The formula for calculating the inhibition rate is as follows: ; Wipe-off sterilization test: The plant-based nourishing makeup remover wipes prepared in Examples 1-3 and Comparative Examples 1-5 were tested for wiping sterilization using the following methods. The plastic carrier was soaked in 75% alcohol for 30 minutes and then dried before use. A bacterial suspension of 1×10⁻⁶ was prepared according to standard laboratory methods. 9 -5×10 9The bacterial strain was Gardnerella vaginalis, purchased from Beina Chuanglian Biotechnology Co., Ltd. (product number BNCC354890). During testing, viable bacterial suspension was simultaneously plate-counted. 0.2 mL of bacterial suspension was naturally dropped onto six carriers and incubated at room temperature for 30 min. One carrier was used, and the bacterial count was recovered by elution and counting, designated as A. Colony recovery method: The carrier was placed in 20 mL of sterile physiological saline and eluted thoroughly. 1 mL of the eluent was used for serial dilution, and plate counting was performed at appropriate dilutions. Two replicates were performed. After incubation and counting, the result was multiplied by the dilution factor and then by 20 to obtain the recovered bacterial count A on the carrier. The other five carriers were wiped with the test sample. Wiping method: Each of the five carriers was wiped 1, 2, 3, 4, and 5 times in the same direction, using a new wet wipe each time, folding the wipe in half once during wiping. Wiping force: Simulate the wiping force of actual makeup remover wipes; take the wiped carrier and collect and count the number of colonies on the carrier by colony recovery method, and count them as C; calculate the wiping sterilization rate, and keep the result to 2 decimal places. The calculation formula for the wiping sterilization rate is as follows. At the same time, record the number of wipings required for the wiping sterilization rate to be greater than 99%. The specific test results are shown in Table 4. ; Table 4. Antibacterial performance tests of the plant-based nourishing makeup remover wipes prepared in Examples 1-3 and Comparative Examples 1-5. As shown in Table 4, the plant-based nourishing makeup remover wipes prepared in Comparative Examples 1-5 and Examples 1-3 of this invention all have excellent antibacterial and bactericidal properties, meeting the needs of practical use.

[0053] This specific embodiment is merely an explanation of the present invention and is not intended to limit the invention. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they are within the scope of the claims of the present invention.

Claims

1. A botanical nourishing makeup removing wet wipe, characterized in that, The wet wipe base material and the makeup removal active component impregnated on the wet wipe base material; The makeup removal active component comprises the following raw materials by weight: 15-20 parts of high-efficiency cleaning component, 2-3 parts of water-retention and moisturizing component, 4-8 parts of plant extraction active component, 0.08-0.16 parts of stabilizer and 84-92 parts of deionized water.

2. The botanical extract nourishing makeup removing wet wipe according to claim 1, wherein, The preparation method of the plant extraction active component comprises the following steps: In step A1, the cactus is washed, crushed, soaked in deionized water, filtered to obtain cactus extract, and then mixed with oil olive leaf extract, soap bark extract, synergistic surfactant and deionized water, and then subjected to enzymatic hydrolysis by adding cellulase to obtain a complex enzymatic hydrolysate, which is sterilized, inoculated with Aspergillus niger for primary fermentation, and then subjected to secondary fermentation by adjusting the pH of the complex fermentation liquor and inoculating with a complex strain, and then subjected to low-temperature sterilization to obtain a plant fermentation product. In step A2, the plant fermentation product, the spilanthes acmella williams extract and the jojoba seed oil are mixed to obtain the plant extraction active component.

3. The botanical extract nourishing makeup removing wet wipe according to claim 2, wherein, In step A1, the mass ratio of the cactus extract, the oil olive leaf extract, the soap bark extract, the synergistic surfactant and the deionized water is 10-16:2.2-3:0.6-0.8:0.2-0.4:

50.

4. The botanical extract nourishing makeup removing wet wipe according to claim 2, wherein, The inoculation amount of the complex strain is 5.6-6.4% of the mass of the complex fermentation liquor.

5. The botanical extract nourishing makeup removing wet wipe according to claim 2, wherein, In step A2, the mass ratio of the plant fermentation product, the spilanthes acmella williams extract and the jojoba seed oil is 9-13:2.6-3.4:1.2-1.

6.

6. The botanical extract nourishing makeup removing wet wipe according to claim 2, wherein, The synergistic surfactant is prepared by the following steps: The chitosan is dissolved in an acetic acid aqueous solution, uniformly stirred, filtered through a microporous filter to obtain a chitosan-acetic acid solution, and then a mixed solution a of octenyl succinic anhydride and anhydrous ethanol is added dropwise, heated to 72-76℃, stirred for 4.2-5.4h, and then the pH value is adjusted to 6.4-6.8, and then subjected to suction filtration, washing and drying to obtain the synergistic surfactant.

7. The botanical extract nourishing makeup removing wet wipe according to claim 6, wherein, The mass ratio of the chitosan, the acetic acid aqueous solution and the mixed solution a is 1:18-24:60-80, and the mass ratio of the octenyl succinic anhydride and the anhydrous ethanol in the mixed solution a is 1-2:

60.

8. A preparation method of the plant extraction nourishing makeup removal wet wipe according to any one of claims 1-7, comprising the following steps: In step S1, the water-retention and moisturizing component is added to deionized water, heated to 40-45℃, and uniformly stirred to obtain an aqueous phase; In step S2, the high-efficiency cleaning component is added to the aqueous phase system, heated to 72-74℃, and stirred for 24-36min to obtain a complex emulsified phase; In step S3, the stabilizer is added to the complex emulsified phase, and stirring is continued for 18-22min, and then the temperature is lowered to 35-40℃, the plant extraction active component is added, and uniformly stirred, and then the pH value is adjusted to 5.5-6.5, and stirring is continued for 8-12min to obtain the makeup removal active component; In step S4, the makeup removal active component is impregnated on the wet wipe base material, and the mass ratio of the makeup removal active component and the wet wipe base material is controlled to be 2-6:1 to obtain the plant extraction nourishing makeup removal wet wipe.

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