Application of selenium-enriched saussurea involucrata fermented Chinese wolfberry primary pulp in preparation of liver protection products

Abstract

The invention relates to the technical field of health intervention food processing, and discloses application of selenium-enriched saussurea involucrata fermented Chinese wolfberry primary pulp in preparation of liver protection products. The product is rich in organic selenium, polyphenol, lactic acid bacteria and other beneficial active components, can effectively improve the activity of antioxidant related enzymes in the liver, reduce the generation of malondialdehyde, relieve oxidative damage of patulin to the liver, and can improve the antioxidant activity of the liver by improving the level of an anti-inflammatory factor IL-10 and reducing the levels of proinflammatory factors IL-6, IL-1beta and TNF-alpha. The inflammatory reaction is relieved. The selenium-enriched saussurea involucrata fermented wolfberry puree provides a potential novel diet strategy for patients with patulin-induced liver injury, and has a considerable injury recovery effect and a wide application prospect.

Description

Technical Field

[0001] This invention relates to the field of processing technology for health intervention foods, specifically to the application of a selenium-enriched snow lotus bacteria fermented wolfberry pulp in the preparation of liver-protecting products. Background Technology

[0002] Patulin (PAT) is a polyketide toxin produced by fungi such as *Penicillium expansum*. It easily contaminates fruits and fruit juices, exhibiting strong neurotoxicity, immunotoxicity, teratogenicity, and carcinogenicity. It also shows toxicity to the intestines, liver, and neuron, seriously threatening human health. Therefore, PAT has been classified as a Group III suspected carcinogen by the International Agency for Research on Cancer. The unsaturated groups in the PAT structure readily react with thiol-containing covalent compounds such as glutathione and cysteine, affecting the dynamic balance of intracellular antioxidant and oxidative reactions, causing oxidative damage and disrupting normal metabolic activities. Selenium, as an important antioxidant trace element, can effectively neutralize free radicals and reduce oxidative stress. However, organisms cannot synthesize selenium themselves and must obtain it through dietary supplementation. Compared to inorganic selenium, organic selenium has the advantages of high bioavailability and low toxicity. Therefore, the development of selenium-enriched foods can concentrate foods with high organic selenium content, making them widely applicable as selenium supplements that can be efficiently utilized by the human body.

[0003] The selenium-enriched fermentation technology of *Symplocos edulis* (a type of fungus) significantly improves the bioavailability of selenium by converting inorganic selenium into organic selenium, and enhances the antioxidant, antibacterial, and gut microbiota-regulating functional properties of *Symplocos edulis*. Goji berries are rich in various active secondary metabolites, such as goji polysaccharides, goji polyphenols, carotenoids, amino acids, microorganisms, and minerals, possessing multiple benefits including improved eyesight, liver protection, antioxidant effects, antibacterial activity, and anti-inflammation. Fermenting goji berry pulp with selenium-enriched *Symplocos edulis* not only enhances the nutritional value of the pulp but also strengthens its antioxidant and anti-inflammatory effects. Currently, no research has been conducted to prepare a *Symplocos edulis*-fermented goji berry pulp with good selenium enrichment effects and to apply it in the field of repairing patulin-induced liver damage. Summary of the Invention

[0004] The purpose of this invention is to provide an application of selenium-enriched snow lotus bacteria fermented wolfberry pulp in the preparation of liver-protecting products. This fermented product is prepared through an optimized selenium-enriched snow lotus bacteria fermentation process. It is rich in organic selenium, polyphenols, lactic acid bacteria, and other beneficial active ingredients. It can effectively increase the activity of antioxidant-related enzymes in the liver, reduce the production of malondialdehyde, alleviate oxidative damage to the liver caused by patulin, and at the same time reduce the inflammatory response by increasing the level of the anti-inflammatory factor IL-10 and decreasing the levels of pro-inflammatory factors IL-6, IL-1β, and TNF-α.

[0005] To achieve the above objectives, this invention provides an application of selenium-enriched snow lotus bacteria fermented wolfberry pulp in the preparation of liver-protecting products.

[0006] Furthermore, the selenium-enriched snow lotus bacteria fermented wolfberry pulp can alleviate the oxidative damage to the liver caused by patulin by increasing the activity of antioxidant-related enzymes in the liver and reducing the production of malondialdehyde.

[0007] Furthermore, the selenium-enriched snow lotus bacteria fermented wolfberry pulp reduces inflammatory responses by increasing the level of anti-inflammatory factor IL-10 and decreasing the levels of pro-inflammatory factors IL-6, IL-1β, and TNF-α.

[0008] Furthermore, the product is a food, functional food, or health product.

[0009] The advantages and positive effects of the selenium-enriched snow lotus bacteria fermented wolfberry pulp described in this invention in the preparation of liver-protecting products are as follows: (1) The selenium-enriched snow lotus bacteria fermented wolfberry pulp provided by the present invention is rich in a variety of beneficial active ingredients such as organic selenium, polyphenols, and lactic acid bacteria. Among them, organic selenium has high bioavailability and low toxicity, which is more conducive to human absorption and utilization. The fermented pulp has excellent antioxidant capacity, which can improve the activity of antioxidant enzymes in the liver, reduce the production of MDA, and effectively alleviate the oxidative damage of patulin to the liver. At the same time, it can reduce the liver inflammatory response by regulating the level of inflammatory factors and regulate the balance of intestinal flora, further enhancing the repair effect on the liver.

[0010] (2) The selenium-enriched snow lotus bacteria fermented wolfberry pulp provided by the present invention can be widely used in food, functional food, health products and other fields, and provides a potential new dietary strategy for patients with liver damage induced by patulin, with considerable damage recovery effect and broad application prospects.

[0011] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0012] Figure 1 The antioxidant capacity indicators of selenium-enriched snow lotus bacteria fermented wolfberry pulp were determined, including DPPH scavenging rate, ABTS scavenging rate, and hydroxyl radical scavenging rate. Figure 2 This invention relates to the effect of selenium-enriched snow lotus bacteria fermented wolfberry pulp on liver tissue in mice with patulin-induced liver injury model. In this invention, A is a liver tissue section of mice in group BC, B is a liver tissue section of mice in group SC, C is a liver tissue section of mice in group PC, and D is a liver tissue section of mice in group SFGJP. Figure 3This invention relates to the effects of selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on common serological indicators of liver injury in mice with patulin-induced liver injury. Specifically, A represents the effect of the selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on alanine aminotransferase (ALT), B represents the effect of the selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on aspartate aminotransferase (AST), C represents the effect of the selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on lactate dehydrogenase (LDH), and D represents the effect of the selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on lipopolysaccharides (LPS). Figure 4 This invention relates to the effects of selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on oxidative stress-related factors in the liver tissue of mice with patulin-induced liver injury. Specifically, A represents the effect of the selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on total antioxidant capacity (T-AOC), B represents the effect of the selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on superoxide dismutase (SOD), C represents the effect of the selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on malondialdehyde (MDA), and D represents the effect of the selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on glutathione peroxidase (GSH-Px). Figure 5 This invention relates to the effects of selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on the levels of inflammatory cytokines in the liver tissue of mice with patulin-induced liver injury. A represents the effect of selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on interleukin-10 (IL-10), B represents the effect of selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on interleukin-6 (IL-6), C represents the effect of selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on interleukin-1β (IL-1β), and D represents the effect of selenium-enriched *Saussurea involucrata* fermented *Lycium barbarum* syrup on tumor necrosis factor α (TNF-α). Detailed Implementation

[0013] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0014] Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention. Experimental methods in the following embodiments that do not specify specific conditions are generally determined according to national standards. Experimental instruments, equipment, and reagents in the following embodiments that do not specify their sources are all commercially available materials.

[0015] Unless otherwise defined or stated, all technical and scientific terms used in this invention have the same meaning as those skilled in the art. Furthermore, any methods and materials similar to or equivalent to those described herein can be applied to the methods of this invention. It should be noted that, unless otherwise specified, the embodiments and features described in this invention can be combined with each other.

[0016] Example 1: Preparation of fermented wolfberry pulp using selenium-enriched snow lotus bacteria I. Experimental Methods: 1. Preparation steps of selenium-enriched snow lotus bacteria fermented wolfberry pulp: (1) Thaw the snow lotus bacteria at 4°C, sieve to obtain bacterial particles, wash the surface of the bacterial particles with sterile water, and then inoculate the bacterial particles into sterile milk and culture at 28°C for 7 consecutive days to activate the snow lotus bacteria.

[0017] (2) Accurately weigh 0.5000g Na2SeO3 and dissolve it in 100mL of distilled water to prepare a 5mg / mL Na2SeO3 solution. Store at 4℃ away from light.

[0018] (3) After sieving and washing the activated snow lotus bacteria, select bacteria particles with similar diameter and growth status, weigh them, and inoculate 2.5% (w / v) snow lotus bacteria into sterile milk and culture it at 28°C.

[0019] (4) After culturing for 2 hours, add a Na2SeO3 solution with a concentration of 5 mg / mL to the bacterial culture to make the Na2SeO3 concentration in the bacterial culture reach 25 μg / mL.

[0020] (5) Continue to incubate at 28℃ for 34 hours, then sieve and wash to obtain selenium-enriched snow lotus bacteria.

[0021] (6) The wolfberry pulp is aseptically filled and provided by Ningxia Huabao Wolfberry Industry Co., Ltd.

[0022] (7) Take 2% selenium-enriched snow lotus bacteria and inoculate it into wolfberry pulp, and ferment it at 28℃ for 36 hours.

[0023] (8) After fermentation, the selenium-enriched snow lotus bacteria were separated by sieve under sterile conditions to obtain the selenium-enriched snow lotus bacteria fermented wolfberry pulp.

[0024] 2. Determination of selenium content in wolfberry pulp fermented with selenium-enriched snow lotus bacteria: This invention uses hydride atomic fluorescence spectrometry to detect selenium content.

[0025] (1) Selenium standard curve: Take 1.00 mL of selenium standard solution (100 mg / L) into a volumetric flask and dilute to volume with 20% hydrochloric acid solution. Set a series of concentrations of selenium standard solution on the instrument (0, 2, 4, 6, 8, 12, 16, 20 μg / L).

[0026] (2) Microwave digestion: freeze drying: The selenium-enriched bacterial particles obtained after sieving and washing were pre-frozen at -80℃ for 12 hours and then freeze-dried under vacuum to constant weight. The mass was recorded and ready for use.

[0027] Digestion: Place the sample in a digestion vessel that has been soaked overnight in 20% nitric acid, add 5 mL of nitric acid, and pre-digest at 110°C for 30 min. After cooling, add 1 mL of nitric acid and 1 mL of hydrogen peroxide, mix well, and seal the vessel for microwave digestion. The microwave digestion procedure is shown in Table 1 below.

[0028] Acid removal: After digestion, open the digestion vessel to release the pressure and transfer it to an electric furnace at 160°C to remove the acid until about 1 mL of clear liquid remains in the digestion vessel.

[0029] Reduction and volume adjustment: After the digestion vessel has cooled, add 5 mL of 50% hydrochloric acid solution and reduce at 110℃ for 30 min. After cooling, transfer to a 50 mL volumetric flask, dilute to volume with water, and mix well before testing. Dilute the sample to the standard range for determination.

[0030] Table 1 Microwave Digestion Procedure

[0031] (3) Sample determination: Blank and sample groups were introduced into the instrument separately, and their fluorescence intensity values ​​were measured and compared quantitatively with the standard series. The unit selenium enrichment amount in the experiment was calculated according to the following formula: ; X This indicates the selenium content (μg / g) in the sample. ρ This indicates the concentration of selenium in the sample (μg / L). ρ 0 This indicates the concentration of selenium in the blank (μg / L). V Indicates the total volume of the sample after final volume adjustment (mL); m This indicates the mass (g) of the sample after freeze-drying.

[0032] 3. Detection and comparison of antioxidant active ingredient indicators in selenium-enriched snow lotus bacteria fermented wolfberry pulp: (1) Three experimental groups were set up: wolfberry pulp group (GJ), wolfberry pulp fermented with snow lotus bacteria group (FGJ), and wolfberry pulp fermented with selenium-enriched snow lotus bacteria group (SFGJ). The antioxidant activity indicators in the three groups of wolfberry pulp were detected and compared.

[0033] (2) Determination of DPPH free radical scavenging rate: 100 μL of fermentation broth diluted 10 times was added to 1 mL of 0.02 mM DPPH solution. A control group (1 mL of anhydrous ethanol and 100 μL of sample) and a blank group (1 mL of DPPH and 100 μL of deionized water) were also set up. After reacting in the dark for 30 min, the absorbance was measured at 517 nm, and the DPPH free radical scavenging rate was calculated using the following formula: ; in, A S Indicates the absorbance value of the sample; A B This represents the absorbance value of the control group; A C This represents the absorbance value of the blank group.

[0034] (3) Determination of ABTS radical scavenging rate: Prepare 7.4 mM ABTS stock solution and 2.6 mM K2S2O8 stock solution. Take 3 mL of each and mix them, vortex to mix well, and react in the dark for 12 h. When using in the experiment, dilute with 95% ethanol solution to make the absorbance at 734 nm 0.7 ± 0.02. Take 200 μL of sample (diluted 50 times) and 1.8 mL of ABTS reaction solution, and set up a blank group (200 μL of deionized water + 1.8 mL of ABTS reaction solution). Measure the absorbance at 734 nm and calculate the ABTS radical scavenging rate using the following formula: ; in, A 1 Indicates the absorbance value of the sample; A 0 This represents the absorbance value of the blank group.

[0035] (4) Determination of hydroxyl radical scavenging rate: 0.5 mL of a 10-fold diluted sample, 0.2 mL of 9 mmol / L FeSO4, and 0.5 mL of 8.8 mmol / L H2O2 were added to a centrifuge tube and vortexed to mix. After reacting for 10 min, 0.5 mL of 9 mmol / L salicylic acid was added to the mixture and the mixture was incubated in a water bath at 37℃ for 10 min. A blank group (with the sample replaced by deionized water) and a control group (with salicylic acid replaced by ethanol) were also set up. Immediately after the reaction was complete, the absorbance was measured at 510 nm, and the hydroxyl radical scavenging rate was calculated using the following formula: ; in, A 0 This represents the absorbance value of the blank group; A 1 This indicates the absorbance value of the sample group; A 2 This represents the absorbance value of the control group.

[0036] II. Experimental Results: 1. Through fermentation, selenium was successfully transferred and converted into the wolfberry pulp. The final test results showed that the total selenium content in the wolfberry pulp fermented with selenium-enriched snow lotus bacteria was 0.92±0.01μg / mL, which meets the standards for selenium-enriched foods, based on the recommended intake of 60μg / d (2023) and the maximum intake of 400μg / d recommended by the Chinese Nutrition Society.

[0037] 2. By measuring DPPH, ABTS, and hydroxyl radical scavenging rate, the antioxidant capacities of GJ, FGJ, and SFGJ can be compared. Their antioxidant capacities can, to some extent, reflect the repair effect of wolfberry juice on patulin-induced liver injury. Figure 1 The results showed that the DPPH radical scavenging rate of SFGJ was 71.15±0.93%, which was 17.50% higher than that of FGJ and 38.88% higher than that of GJ (p<0.05). The ABTS radical scavenging rate of SFGJ was 64.77±0.54%, which was 9.53% higher than that of FGJ and 31.18% higher than that of GJ (p<0.05). The hydroxyl radical scavenging ability was significantly increased by 9.99% after selenium-enriched fermentation compared to FGJ (p<0.05). This indicates that the antioxidant capacity of selenium-enriched fermented wolfberry pulp is greatly enhanced.

[0038] Example 2: Repairing effect of selenium-enriched snow lotus bacteria fermented wolfberry pulp on patulin-induced liver injury I. Experimental Methods: 1. Laboratory animals: The experimental mice were male C57BL / 6J (6 weeks old) purchased from Chongqing Tengxin Biotechnology Co., Ltd. The mice were housed in a standard animal facility with a stable temperature of 22±2℃ and humidity of 50±5%. A 12-hour light / dark cycle was implemented, and the mice had free access to food and water throughout the feeding process. The bedding was changed every two days to maintain a clean living environment for the experimental animals.

[0039] 2. Grouping and setup of experimental mice: (1) Construction of a mouse model of liver injury induced by patulin: Mice with uniform body weight were randomly divided into 4 groups of 12 mice each. Animal models were established according to Table 2. The entire experiment lasted for 24 days, and body weight was recorded every 3 days. After treatment on day 25, the mice were fasted and deprived of water for 24 hours. Blood was then collected, the mice were sacrificed, and the livers were collected and weighed. Part of the liver tissue was cut off and transferred to 4% paraformaldehyde for fixation.

[0040] Table 2. Establishment and treatment of animal models

[0041] 3. Liver histological analysis: Liver tissue fixed with 4% paraformaldehyde was fixed at 4°C for 24 hours. After fixation, the tissue was dehydrated with ethanol, embedded in paraffin, and sectioned. The sections were stained with hematoxylin and eosin (H&E) and observed using a fluorescence inverted microscope.

[0042] 4. Measurement of serological markers and oxidative stress markers: Serum ALT, AST, LDH, and LPS were measured according to the kit method; liver GSH-Px, SOD, MDA, T-AOC, and GSH were measured according to the kit method.

[0043] 5. Measurement of serum inflammatory factors: Serum TNF-α, IL-1β, IL-10, and IL-6 were measured according to the kit instructions.

[0044] II. Experimental Results: 1. Selenium-enriched snow lotus bacteria fermented wolfberry pulp improves liver tissue morphology in mice: The results are as follows Figure 2 As shown, no obvious pathological changes were observed in the liver sections of mice in groups BC and SC. Hepatic cords centered on the central vein were observed to be arranged radially and orderly, with clear and plump cell nuclei and tightly and orderly arranged cells. Figure 2 (A and B in the text). This indicates that the intake of selenium-enriched snow lotus bacteria fermented wolfberry pulp alone does not induce liver tissue lesions. However, inflammatory cell infiltration was clearly observed in the hepatocytes of the PC group mice, accompanied by localized darkening of staining, i.e., increased cytoplasmic eosinophilia, indicating hepatocyte apoptosis or necrosis. In addition, some cell nuclei showed signs of lysis (…). Figure 2 C in the text. Mice treated with selenium-enriched snow lotus bacteria fermented wolfberry pulp showed only mild vacuolar degeneration and increased nuclear volume in liver tissue sections, with no inflammatory cell infiltration observed. Figure 2 (D in the text). This indicates that the selenium-enriched snow lotus bacteria fermented wolfberry pulp can effectively repair hepatocellular tissue lesions caused by patulin.

[0045] 2. Selenium-enriched snow lotus bacteria fermented wolfberry pulp improves serological indicators in mice with patulin-induced liver injury: ALT, AST, LDH, and LPS are four common serological markers for detecting liver injury. Among them, LDH levels rise when the liver develops cirrhosis, hepatitis, or chronic active hepatitis, while LPS can reflect the degree of hepatocellular damage. Results are as follows... Figure 3 As shown, there was no significant difference in serum ALT and AST levels between the SC group and the BC group, indicating that SFGJ alone had no effect on serum ALT and AST levels in mice. In mice treated with PAT, serum ALT and AST levels increased by 61.34% and 56.40% respectively compared to the BC group (p<0.05), while serum ALT levels decreased by 26.61% (p<0.05) and AST levels decreased by 32.08% (p<0.05) in the SFGJP group. Figure 3 (A and B in the text). Similarly, there was no significant difference in serum LDH and LPS levels between the SC group and the BC group. In the PC group, serum LDH and LPS levels increased by 28.88% and 14.78%, respectively (p<0.05). Compared with the PC group, LDH and LPS levels in the SFGJP group decreased by 17.27% and 8.46%, respectively (p<0.05). Figure 3 (C and D in the text).

[0046] The above results preliminarily demonstrate that dietary intake of selenium-enriched snow lotus bacteria fermented wolfberry pulp can play a good role in repairing and protecting against patulin-induced liver damage.

[0047] 3. Selenium-enriched snow lotus bacteria fermented wolfberry pulp enhances the antioxidant capacity of mouse liver tissue: The results are as follows Figure 4 As shown, compared with the SC group, the antioxidant capacity of the liver tissue of mice in the PC group was significantly reduced, inducing oxidative stress in the liver. After ingestion of selenium-enriched snow lotus fermented wolfberry pulp, the activities of SOD and GSH-Px enzymes in the liver tissue of mice were significantly increased compared with the PC group (p<0.05). The level of MDA is related to the lipid peroxidation reaction that occurs in liver disease, and the MDA level was significantly decreased in the SFGJP group. This indicates that selenium-enriched snow lotus fermented wolfberry pulp can effectively improve the antioxidant level of mouse liver and repair oxidative damage to liver tissue.

[0048] 4. Selenium-enriched snow lotus bacteria fermented wolfberry pulp inhibits inflammation in mouse liver tissue: The results are as follows Figure 5As shown, compared with the BC group, the serum levels of the anti-inflammatory factor IL-10 in the PC group mice decreased by 44.72% (p<0.05), while the levels of pro-inflammatory factors IL-6, IL-1β, and TNF-α were significantly increased. Treatment with selenium-enriched *Syzygium aromaticum* fermented wolfberry pulp effectively alleviated the inflammatory response in mouse liver tissue, reduced pro-inflammatory factor levels, and increased anti-inflammatory factor levels. Therefore, dietary supplementation with selenium-enriched *Syzygium aromaticum* fermented wolfberry pulp to regulate liver antioxidant and inflammatory factor levels is a possible approach to repair patulin-induced liver damage.

[0049] This invention demonstrates through gavage experiments on a mouse model of liver injury induced by patulin that fermented wolfberry pulp with selenium-enriched snow lotus bacteria can effectively alleviate liver damage and reduce the level of inflammatory factors in the mouse model, and enhance the antioxidant capacity of liver tissue.

[0050] Therefore, the selenium-enriched snow lotus-infused fermented wolfberry pulp of this invention is rich in organic selenium, polyphenols, lactic acid bacteria, and other beneficial active ingredients. It can effectively increase the activity of antioxidant-related enzymes in the liver, reduce malondialdehyde production, and alleviate oxidative damage to the liver caused by patulin. Simultaneously, it reduces the inflammatory response by increasing the level of the anti-inflammatory factor IL-10 and decreasing the levels of pro-inflammatory factors IL-6, IL-1β, and TNF-α. This selenium-enriched snow lotus-infused fermented wolfberry pulp provides a potential novel dietary strategy for patients with patulin-induced liver injury, demonstrating considerable damage recovery effects and broad application prospects.

[0051] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. Application of a selenium-enriched snow lotus bacteria fermented wolfberry pulp in the preparation of liver-protecting products.

2. The application according to claim 1, characterized in that, The selenium-enriched snow lotus bacteria fermented wolfberry pulp alleviates the oxidative damage to the liver caused by patulin by increasing the activity of antioxidant-related enzymes in the liver and reducing the production of malondialdehyde.

3. The application according to claim 1, characterized in that, The selenium-enriched snow lotus bacteria fermented wolfberry pulp reduces inflammatory responses by increasing the level of anti-inflammatory factor IL-10 and decreasing the levels of pro-inflammatory factors IL-6, IL-1β, and TNF-α.

4. The application according to claim 1, characterized in that, The product is a food, functional food, or health product.

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