Tightening and anti-wrinkle mask containing ginseng tissue culture nano-emulsion and preparation method of firming and anti-wrinkle mask

Abstract

The invention provides a firming and anti-wrinkle mask containing ginseng tissue culture nano-emulsion and a preparation method of the firming and anti-wrinkle mask, and relates to the technical field of ginseng tissue culture nano-carriers. The mask essence is prepared from the following components in parts by weight: 0.5 to 3 parts of ginseng tissue culture nano emulsion, 5 to 15 parts of polyhydric alcohol, 0.1 to 0.3 part of grease, 0.05 to 0.5 part of perigord truffle extract, 0.001 to 0.01 part of anti-wrinkle polypeptide, 0.05 to 0.2 part of dipotassium glycyrrhizinate, 0.05 to 0.15 part of hydrolyzed sodium hyaluronate, 0.1 to 1 part of p-hydroxyacetophenone, 0.1 to 1 part of 1, 2-hexanediol, 0.1 to 0.5 part of hydroxyethyl cellulose and 0.2 to 0.5 part of PEG-40 hydrogenated castor oil. The total weight part is 100, and the mask cloth is ginseng nanofiber membrane cloth. According to the patch type facial mask containing the ginseng tissue culture nano-emulsion and the ginseng nanofiber membrane cloth provided by the invention, through a two-stage permeation enhancing system and a functional component synergistic mechanism, triple improvement of bioavailability, transdermal efficiency and anti-wrinkle effect which are difficult to break through by a traditional facial mask technology is realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of ginseng tissue culture nanocarrier, more specifically, it is a ginseng tissue culture nanolipid emulsion firming anti-wrinkle mask and a preparation method thereof. BACKGROUND

[0002] Patch mask is a mainstream skin care product, the core of which is composed of a mask cloth carrying essence liquid, which is used to cover the face, and through the physical occlusion effect (temporary skin temperature rise, promoting pore relaxation) of the mask cloth and the infiltration of the essence liquid, efficient penetration and intensive nourishment of active ingredients are achieved, thereby achieving the effects of rapid hydration, repair, skin lightening and the like, and it is one of the important choices for modern convenient and efficient skin care. With the development of the cosmetic industry, the traditional ordinary mask essence liquid plus non-woven carrier mask cloth product has been unable to meet the market demand. The patch mask market is developing and changing from the traditional basic care carrier to the technological efficacy skin care direction, in order to improve the absorption and utilization rate of efficacy ingredients and better product use experience, and meet the market consumers' requirements for better efficacy, safety and environmental protection of cosmetic products.

[0003] In the prior art, the scheme of loading ginseng as an active efficacy ingredient includes: (1) Setting a ginseng mask essence liquid layer in the mask: a typical scheme of this type is disclosed in Chinese Utility Model Patent No. CN219230797U, which discloses a face mask cloth body provided with a face through hole assembly, the face mask cloth body including an outer cloth layer, a first silk layer, a moisturizing layer, a ginseng mask essence liquid layer and a second silk layer, and the face mask cloth body is provided with a face sticking assembly, and the ginseng mask essence liquid layer is a ginseng freeze-dried powder sodium hyaluronate gel layer.

[0004] This scheme can effectively load ginseng freeze-dried powder material, and there is no loss of ginseng efficacy material in the preparation process, but this scheme has the problem that the ginseng efficacy material is difficult to penetrate and absorb, and only the moisturizing effect is improved.

[0005] (2) Loading an essence liquid containing ginsenosides in the mask: a typical scheme of this type is disclosed in Chinese Invention Patent No. CN109984969A, which discloses a preparation method of a ginseng whitening mask, including crushing, grinding, filtering and centrifuging fresh undergrowth ginseng to obtain undergrowth ginseng serum, i.e. fresh ginseng extract; the preparation method of the whitening mask is: homogenizing the fresh ginseng extract, oil phase, water phase, emulsifier, co-emulsifier and / or nutritional ingredients by high-pressure microfluidization to prepare a sleep mask.

[0006] The extraction method of fresh ginseng extract used in this scheme is to extract the liquid by grinding, which has insufficient extraction capacity for active ingredients in ginseng, resulting in waste of ginsenosides.

[0007] (3) Use ginseng fiber to prepare mask cloth, and use essence containing ginsenosides at the same time: a typical scheme of this type is disclosed in Chinese patent application CN 117618308 A, which discloses a mask base cloth comprising 8-12 parts of chitosan fiber, 5-10 parts of silk fiber, 6-10 parts of spinning fiber, 3-5 parts of ginseng fiber; the spinning fiber comprises 2-3 parts of gelatin, 0.2-0.4 parts of hyaluronic acid powder, and 0.03-0.08 parts of ginseng polysaccharide powder; the ginseng fiber comprises ginseng hemicellulose; the mask liquid comprises water, moisturizing and hydrating ingredients, glycerol, propylene glycol, thickening agent, chelating agent, preservative, triethanolamine, and ginseng stem and leaf total saponins.

[0008] In this scheme, ginseng stem and leaf total saponins are directly mixed in the mask liquid, which does not solve the problem of the difficulty of transdermal penetration and absorption of ginsenosides as macromolecular active ingredients. SUMMARY

[0009] To solve the above problems, the technical scheme adopted by the present application is a ginseng tissue culture nanometer emulsion firming and anti-wrinkle mask, which comprises a mask cloth and a mask essence, and is characterized in that: The mask essence comprises the following components by weight: 0.5-3 parts of ginseng tissue culture nanometer emulsion, 5-15 parts of polyhydric alcohol, 0.1-0.3 parts of oil, 0.05-0.5 parts of black truffle extract, 0.001-0.01 parts of anti-wrinkle polypeptide, 0.05-0.2 parts of di-potassium glycyrrhizinate, 0.05-0.15 parts of hydrolyzed sodium hyaluronate, 0.1-1 parts of p-hydroxyacetophenone, 0.1-1 parts of 1,2-hexanediol, 0.1-0.5 parts of hydroxyethyl cellulose, 0.2-0.5 parts of PEG-40 hydrogenated castor oil, and the balance is purified water, with a total weight of 100 parts.

[0010] Alternatively, the ginseng tissue culture nanometer emulsion comprises, by weight: 0.5-1.5 parts of ginseng tissue culture, 3-5 parts of butanediol, 1-3 parts of squalane, 0.5-1.5 parts of polysorbate-20, 0.5 parts of p-hydroxyacetophenone, and the balance is purified water, with a total weight of 100 parts; the average particle size of the nanometer emulsion is 100-200 nm, and the particle size distribution coefficient of the nanometer emulsion is less than 0.2 in terms of polydispersity index.

[0011] Alternatively, the ginseng tissue culture is added in the form of a ginseng tissue culture solution, and the weight of the ginseng tissue culture in the nanometer emulsion is calculated based on the actual content of the ginseng tissue culture in the ginseng tissue culture solution; The preparation of the ginseng tissue culture solution comprises: freezing ginseng tissue culture, homogenizing after thawing, adjusting pH of the solution, adding enzyme under temperature control, enzymolysis, inactivating enzyme after the completion of enzymolysis, centrifugation, and reserving supernatant; adding water to the precipitate after centrifugation, reflux extraction, filtration, adding ethanol to the filtrate, and standing, and combining the precipitate and the supernatant to obtain the ginseng tissue culture solution.

[0012] Optionally, the preparation process of the ginseng tissue culture nanoemulsion comprises the following steps: S101: weighing squalane, polysorbate-20 and butanediol, stirring and mixing uniformly, and then warming to 65-75 DEG C for standby, to obtain an oil phase; S102: weighing purified water, p-hydroxyacetophenone and ginseng tissue culture solution, stirring and mixing uniformly, and then warming to 65-75 DEG C for standby, to obtain an aqueous phase; S103: under temperature control and stirring, adding the aqueous phase into the oil phase, the temperature control temperature is 65-75 DEG C, after the completion of the addition, homogenizing for 3-10 minutes, to obtain a coarse emulsion; the stirring speed is 300-500 r / min; the homogenizing speed is 2000-4000 r / min, and the temperature is 65-75 DEG C; S104: cooling the coarse emulsion to below 45 DEG C, high-pressure homogenization for 5 times, the homogenization pressure is 80 MPa, to obtain the ginseng tissue culture nanoemulsion.

[0013] Optionally, the mask cloth is a ginseng nanofiber mask cloth, and the preparation of the ginseng nanofiber mask cloth comprises: chemically modifying inorganic nanocarbon materials to obtain modified inorganic nanocarbon material slurry; adding a dispersant into the modified inorganic nanocarbon material slurry to disperse, to obtain inorganic nanocarbon dispersion liquid; preparing ginsenoside solution; dispersing the ginsenoside solution into the inorganic nanocarbon dispersion liquid to disperse, to obtain inorganic-organic mixed dispersion liquid; first mixing the inorganic-organic mixed dispersion liquid with cellulose spinning solution to obtain composite dispersion liquid; second mixing the composite dispersion liquid with cellulose spinning solution to obtain composite spinning glue; spinning the composite spinning glue by wet spinning to obtain inorganic-organic material synergistic high-sensitivity fiber, and finally spinning into yarn through weaving, and weaving into ginseng nanofiber fabric through double-sided circular knitting machine.

[0014] Optionally, the inorganic nanocarbon material is one or more of fullerene, graphene, carbon nanotube and carbon black, and the cellulose spinning solution is modal fiber spinning solution, and the mass fraction ratio of ginseng multifunctional powder to cellulose spinning solution in the first mixing is 3:7.

[0015] Optionally, the polyhydric alcohol is a mixture of one or more of butanediol, glycerol, methylpropylene glycol and polyethylene glycol-8.

[0016] Optionally, the oil is a mixture of one or more of white pool seed oil, olea europaea fruit oil, helianthus annuus seed oil, simmondsia chinensis seed oil, vitis vinifera seed oil.

[0017] Optionally, the anti-wrinkle polypeptide is a mixture of one or more of tripeptide-1, palmitoyl pentapeptide-4, hexapeptide-9, arginine / lysine polypeptide, carnosine, acetyl hexapeptide-8, acetyl tetrapeptide-7, acetyl tetrapeptide-9. The application also provides a preparation process of a ginseng tissue culture nanometer emulsion firming and anti-wrinkle mask, characterized in that it is suitable for preparing the ginseng tissue culture nanometer emulsion firming and anti-wrinkle mask according to any one of the preceding description, and comprises the following steps: S201: polyhydric alcohol, hydroxyethyl cellulose, and sodium hyaluronate are added into 75 parts of purified water, stirred and dispersed uniformly, and heated to 75-80 DEG C at the same time, and after stirring until completely dissolved, the temperature is lowered; S202: when the temperature is lowered to 55-60 DEG C, p-hydroxyacetophenone and 1,2-hexanediol are added, stirred and dissolved, and then the temperature is continuously lowered; S203: when the temperature is lowered to below 40 DEG C, dipotassium glycyrrhizinate, oil, PEG-40 hydrogenated castor oil, anti-wrinkle polypeptide, black truffle extract, and ginseng tissue culture nanometer emulsion are added under stirring, stirred until completely dispersed uniformly, and the total weight parts are made up to 100 parts with purified water; the temperature is lowered to below 35 DEG C to obtain a mask essence; S204: the mask essence and ginseng nanofiber mask cloth are filled into a mask bag and sealed to prepare a patch type mask.

[0018] The ginseng tissue culture nanometer emulsion firming and anti-wrinkle mask and the preparation method thereof have the following beneficial effects: The patch type mask containing the ginseng tissue culture nanometer emulsion and the ginseng nanofiber mask cloth provided by the application realizes the triple improvement of bioavailability, transdermal efficiency and anti-wrinkle effect which cannot be achieved by traditional mask technology through the synergistic mechanism of the double-stage penetration system and the functional ingredients, and specifically embodies the following aspects: (1) the ginseng tissue culture nanometer emulsion with a particle size of 100-200 nm is used as the raw material of the mask essence, the transdermal absorption rate and absorption speed of rare ginsenosides are improved, and the industry problem that macromolecular ginsenosides are difficult to penetrate the skin barrier is overcome; (2) the oxygen anions and micro-current released by the ginseng nanofiber mask cloth can improve the transdermal rate of active ingredients in the essence by reducing the Zeta potential of the skin surface and expanding the intercellular space, and form a time-space synergistic effect of "nanometer carrier + physical penetration"; (3) Anti-wrinkle efficacy index enhancement: Nanoemulsion protects ginsenoside Rg3 / Rb1 and other high-efficiency penetration into the dermis layer, promotes the production of type I collagen, and removes ROS active oxygen in the skin to achieve the effect of tightening and anti-wrinkles; At the same time, the film cloth continuously activates the skin microcirculation with micro-current, accelerates the removal of metabolic waste and the absorption and utilization of efficacy nutrients; At the same time, it improves the biocompatibility of the cosmetic product and the human body, the nanoemulsion encapsulation rate is ≥80%, avoids the stimulation of chemical penetration enhancers, and the film cloth has no sensitization phenomenon through human body experience test. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or prior art description will be briefly introduced.

[0020] Figure 1 is the particle size light intensity distribution diagram of the nanoemulsion containing ginseng tissue culture prepared immediately before testing provided by the embodiments of the present application; Figure 2 is the particle size light intensity distribution diagram of the nanoemulsion containing ginseng tissue culture prepared and stored at 25℃ environment for 90 days before testing provided by the embodiments of the present application; Figure 3 is the particle size light intensity distribution diagram of the nanoemulsion containing ginseng tissue culture prepared and stored at 40℃ / 75% R.H. environment for 90 days before testing provided by the embodiments of the present application; Figure 4 is the liquid chromatogram of the ginseng tissue culture encapsulation rate detection of the sample to be tested provided by the embodiment 1 of the present application; Figure 5 is the liquid chromatogram of the ginseng tissue culture encapsulation rate detection of the control sample provided by the embodiment 1 of the present application. DETAILED DESCRIPTION

[0021] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application more clearly understood, the present application will be further described in detail below in combination with the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, and are not used to limit the present application.

[0022] Example 1: The mask essence is composed of the following ingredients: 1.5 parts of ginseng tissue culture nanoemulsion, 13 parts of polyhydric alcohol (including 2 parts of butanediol, 5 parts of glycerol and 6 parts of methyl propylene glycol), 0.2 parts of oil and fat (including 0.1 part of white pool seed oil, 0.05 part of olive fruit oil, 0.05 part of sunflower seed oil), 0.25 parts of black truffle extract, 0.005 parts of anti-wrinkle polypeptide (including tripeptide-1, palmitoyl pentapeptide-4 and hexapeptide-9 at a mass ratio of 1:1:1), 0.1 parts of disodium glycyrrhizinate, 0.1 parts of sodium hyaluronate, 0.5 parts of p-hydroxyacetophenone, 0.5 parts of 1,2-hexanediol, 0.3 parts of hydroxyethyl cellulose, and the rest is purified water, with a total weight of 100 parts; the mass parts of each raw material are calculated based on pure products.

[0023] The ginseng nanofiber fabric uses ginseng adventitious root extract, is mixed with inorganic nanocarbon material, is prepared, and then obtains an inorganic-organic nanometer mixed dispersion liquid containing ginseng fibers. Then the dispersion liquid is added to the cellulose spinning solution, and after treatment, a composite spinning glue is obtained. Then, using a wet spinning process, a high-sensitivity fiber with inorganic-organic material structure-activity synergy is obtained. Finally, it is woven into yarn, knitted on a double-needle circular knitting machine, and finally ginseng nanofiber fabric is obtained. The ginseng nanofiber fabric is cut into a facial mask shape.

[0024] The mask essence and the ginseng nanofiber mask cloth are filled into the mask bag and sealed to make a patch type mask.

[0025] The preparation of the ginseng nanofiber fabric includes: The inorganic nanocarbon material is chemically modified to obtain a modified inorganic nanocarbon material slurry.

[0026] In deionized water, 20% of fullerene is mixed and stirred, then 2% of the modifier is added and stirred, the temperature is 30°C, after adding the modifier, stirring for 7 hours, the stirring speed is 3000 revolutions / minute, to prepare the modified inorganic nanocarbon material slurry, the particle size of fullerene is required to be D90≤10 microns, the mass percentage of fullerene and modifier is based on the total mass of the inorganic nanocarbon material slurry as the measurement basis, and the modifier includes silane coupling agent and polyvinylpyrrolidone at a mass ratio of 1:1.

[0027] In this embodiment, the silane coupling agent uses 3-(trimethoxysilyl) methyl propyl methacrylate (purity 98%, boiling point 190°C, density 1.045 g / mL at 25°C) from Merck.

[0028] The dispersant is added to the modified inorganic nanocarbon material slurry for dispersion to obtain an inorganic nanocarbon dispersion liquid.

[0029] Squalane is added to the modified inorganic nanocarbon material slurry, and stirring is performed while adding, the stirring speed is 3000 revolutions / minute, to accelerate dispersion, after the addition is completed, the mass percentage of squalane is 2%, the temperature is kept at 40°C, and stirring is performed for 6 hours to obtain an inorganic nanocarbon dispersion liquid, and the mass percentage of squalane is taken as the measurement basis of the total mass of the inorganic nanocarbon dispersion liquid.

[0030] A ginsenoside solution is prepared.

[0031] The ginseng extract is a ginseng adventitious root extract, and the ginseng adventitious root extract cultured by the plant stem cell technology of Anran Nano is micronized, and the culture method of the ginseng adventitious root is referred to the authorized patents of Anran Nano (patent numbers ZL202110061539.8, ZL202110061540.0, ZL202110361903.2, ZL202110360926.1, and ZL202110365313.7).

[0032] The preparation method of the ginseng extract is performed according to the authorized patent of Anran Nano with the authorized announcement number CN115813967B, and in this embodiment, the technical index of the ginseng extract is that the total ginsenoside content in the ginseng extract is 75%, the drying loss is 1.6%, and the particle size is less than 80 mesh.

[0033] The ginseng extract is added to deionized water, and an ultrasonic processor is used to fully dissolve the ginseng extract, the ultrasonic power is 400w, and the time is 20 minutes. At the same time, the temperature is kept at 40°C to obtain a ginsenoside solution, and the mass fraction of the ginseng extract in the ginseng extract solution is 50%.

[0034] The ginsenoside solution is added to the inorganic nanocarbon dispersion liquid to disperse, and an inorganic-organic mixed dispersion liquid is obtained.

[0035] The ginsenoside solution is added to the inorganic nanocarbon dispersion liquid, and stirring is performed while adding, the stirring is continuously performed for 2h, and the speed is 600 revolutions / minute, so that the ginsenoside is uniformly coated in the inorganic nanocarbon material in the process of slow agglomeration, thereby obtaining an inorganic-organic mixed dispersion liquid, and the mass ratio of the ginsenoside solution to the inorganic nanocarbon dispersion liquid is 1:1.

[0036] The inorganic-organic mixed dispersion liquid is mixed with the cellulose spinning liquid for the first time to obtain a composite dispersion liquid.

[0037] The inorganic-organic mixed dispersion liquid is mixed with the cellulose spinning liquid once, and the mass fraction ratio of the inorganic-organic mixed dispersion liquid to the cellulose spinning liquid in the first mixing is 30%:70%, a composite dispersion liquid is obtained, a high-speed stirrer is stirred for 0.5h, the speed is 3000 revolutions / minute, and the standing time is 6h. The cellulose spinning liquid is modal fiber spinning liquid.

[0038] The composite dispersion liquid is mixed with the cellulose spinning solution for the second time to obtain a composite spinning dope.

[0039] The composite dispersion liquid is mixed with the cellulose spinning solution for the second time, and the composite dispersion liquid and the cellulose spinning solution are mixed at a ratio of 1:10. The mixture is stirred by a high-speed blender for 0.5 h at a speed of 3000 r / min to obtain a composite spinning dope, which is left for 2 h. The type of the cellulose spinning solution in step S6 is consistent with that in step S5.

[0040] The composite spinning dope is spun by a wet spinning method to obtain an inorganic-organic material structure-activity synergistic high-sensitivity fiber.

[0041] First, the composite spinning dope is filtered by three-stage progressive filtration of 100 μm, 50 μm and 20 μm to remove residual mechanical impurities and small gel particles. Then, the composite spinning dope is left for 18-24 h at 80-90 °C in a vacuum environment to eliminate bubbles in the liquid. Subsequently, the composite spinning dope is precisely controlled in flow rate by a metering pump and delivered to a spinneret assembly. The spinneret is provided with fine holes, and the composite spinning dope is uniformly extruded from the fine holes under pressure. The yarn stream first passes through an air gap zone and is stretched under traction. Subsequently, the yarn stream enters a coagulation bath at 20-40 °C, and the liquid component of the coagulation bath is a 15% NMMO aqueous solution by mass fraction. The yarn bundle stays in the coagulation bath for 15 s, and the total mass ratio of the yarn bundle to the coagulation bath liquid is 1:100 to obtain a nascent yarn.

[0042] The nascent yarn is drawn to a water washing unit by a godet, and is washed by 8 stages of countercurrent washing. The water washing temperature is 60 °C, the water washing bath ratio is 1:50, and the washing time of each stage is 60 s.

[0043] The yarn after water washing enters an oiling tank to adhere to an oil agent with a mass fraction of 2%. The oil agent is an oil agent SM-YJ-80 from Shaoxing Shangyu Smo Organic Chemical Research Institute (effective content ≥80.5%, moisture content ≤19.5%, and pH value of 1% aqueous solution is 7.1), and the solvent of the oil agent is deionized water. The oiling temperature is 30 °C, and the oiling rate is 0.6%.

[0044] The yarn after oiling enters a hot air circulating dryer to be dried at a temperature of 70 °C so that the water content of the fiber is reduced to ≤5%.

[0045] The prepared fiber has a specification of 1.2 D x 38 mm.

[0046] The preparation of the ginseng nanoemulsion includes: The ginseng nanoemulsion provided in the example includes ginseng tissue culture 1 part, butanediol 4 parts, squalane 2 parts, polysorbate-20 1 part, and p-hydroxyacetophenone 0.5 part by weight parts, and the rest is purified water. The specific preparation method is as follows: S101: weigh squalane, polysorbate-20, butanediol, and mix them evenly by mechanical stirring, and heat to 70-75℃ for standby; S102: weigh purified water, p-hydroxyacetophenone, and ginseng tissue culture solution, stir them evenly, and heat to 70-75℃, wherein the concentration of the ginseng tissue culture solution is 2.8%, that is, the addition amount of the pure ginseng tissue culture is 1 part, and the actual addition amount of the ginseng tissue culture solution is 35.71 parts.

[0047] S103: under heat preservation and rapid stirring, add the water phase to the oil phase, stir and mix evenly, and the heat preservation temperature is 70-75℃, during the process of adding the water phase to the oil phase, the adding mode is slow at first and then fast, that is, the first 1 / 3 of the water phase material is added at a speed of 0.1L / min, after stirring evenly, the remaining water phase is directly added to the oil phase, and then homogenized at normal pressure for 5 minutes to obtain a coarse emulsion; the stirring speed is 600r / min; the homogenization speed is 2000r / min, and the normal pressure homogenization temperature is 70-75℃.

[0048] S104: cool the coarse emulsion to below 45℃, and high-pressure homogenize for 6 times with a homogenization pressure of 70MPa to obtain a ginseng tissue culture nanoemulsion.

[0049] The ginseng tissue culture is added in the form of a ginseng tissue culture solution, and the weight parts of the ginseng tissue culture in the nanoemulsion are calculated according to the actual content of the ginseng tissue culture in the ginseng tissue culture solution.

[0050] The ginseng tissue culture is a tissue culture ginseng adventitious root, which is obtained by using the method in the prior art. The ginseng tissue culture in the present application can be obtained by using the method for culturing ginseng adventitious roots in the granted patents CN112931216B, CN112806267B and CN113186150B.

[0051] The ginseng tissue culture in the present embodiment is obtained by using the method for culturing ginseng adventitious roots in the granted patent CN112931216B.

[0052] The preparation of the ginseng tissue culture solution is carried out by using the method for preparing a ginseng tissue culture extract in the published patent CN118830620A, which comprises: freezing the ginseng tissue culture, homogenizing after thawing, adjusting the pH of the solution, adding enzymes under temperature control, enzymolysis for a certain time, enzyme inactivation after enzymolysis is completed, centrifugation, adding water to the precipitate after centrifugation, reflux extraction, filtration, adding ethanol to the filtrate, standing, and combining the precipitate and the supernatant to obtain the ginseng tissue culture solution.

[0053] The specific steps are as follows: (1) freeze-thaw: take 15g of ginseng adventitious roots, Freeze at 80℃ for 12 hours, thaw and add 20 times the volume of water, homogenize and adjust the pH of the solution to 5; (2) Enzymatic hydrolysis: Add 0.4% of a compound enzyme at 50℃. The mass ratio of cellulase: pectinase: β-glucosidase in the compound enzyme is 1:1:1. Hydrolyze for 120 min. After hydrolysis, inactivate the enzyme, centrifuge, and take the supernatant for later use.

[0054] (3) Reflux extraction: Add 20 times the volume of water to the precipitate after centrifugation, reflux at 100℃ for 120 minutes, filter after extraction, add 95% ethanol to the filtrate to make the final mass concentration reach 70%, precipitate overnight, centrifuge to obtain crude polysaccharide precipitate, dissolve in water, combine the supernatant after enzymatic hydrolysis and centrifugation to obtain ginseng tissue culture solution.

[0055] The concentration of the ginseng tissue culture solution was calculated based on the amount of ginseng tissue culture added. The ginseng tissue culture solution was concentrated to 2.8% by mass using a rotary evaporator. This solution was then used in experiments (the amount of ginseng tissue culture added was 15g, and the mass of the ginseng tissue culture solution was the sum of the mass of the supernatant extracted after enzymatic hydrolysis, the mass of the water used to dissolve the crude polysaccharide precipitate after reflux extraction, and the mass of the crude polysaccharide precipitate. When the amount of ginseng tissue culture added exceeded 2.8% by mass, purified water was added and stirred for dilution).

[0056] The preparation process of ginseng tissue culture nanoemulsion firming and anti-wrinkle facial mask includes the following steps: S201: Add polyol, hydroxyethyl cellulose, and hydrolyzed sodium hyaluronate to 75 parts of purified water, stir to disperse evenly, and simultaneously heat to 75-80℃. Stir until completely dissolved, then cool down. S202: Cool to 55-60℃, add p-hydroxyacetophenone and 1,2-hexanediol, stir to dissolve, and continue cooling; S203: When the temperature drops to below 40℃, add dipotassium glycyrrhizate, oil, PEG-40 hydrogenated castor oil, anti-wrinkle peptides, black truffle extract, and ginseng tissue culture nanoemulsion while stirring. Stir until completely dispersed and evenly mixed. Use purified water to make up the total weight to 100 parts. Cool to below 35℃ to obtain the mask essence. S204: Fill the mask essence and ginseng nanofiber mask cloth into a mask bag and seal it to make a sheet mask.

[0057] Example 2: The difference from Example 1 is that in this example, the mask essence is composed of the following ingredients: 0.5 parts ginseng tissue culture nanoemulsion, 5 parts polyol (including 2 parts butylene glycol and 3 parts polyethylene glycol), 0.1 parts oil (including 0.05 parts jojoba seed oil and 0.05 parts grape seed oil), 0.1 parts black truffle extract, 0.001 parts anti-wrinkle peptide (including arginine / lysine peptide and carnosine in a mass ratio of 1:1), 0.05 parts dipotassium glycyrrhizate, 0.05 parts hydrolyzed sodium hyaluronate, 0.1 parts p-hydroxyacetophenone, 0.1 parts 1,2-hexanediol, 0.1 parts hydroxyethyl cellulose, 0.2 parts PEG-40 hydrogenated castor oil, and the remainder is purified water, with a total weight of 100 parts. In the preparation of the ginseng nanofiber membrane: the inorganic nanocarbon material is a mixture of graphene and carbon nanotubes in a mass ratio of 1:1, and the particle size of graphene and carbon nanotubes is required to be D90≤10 micrometers. The remaining composition and preparation process of the ginseng nanofiber membrane are the same as in Example 1.

[0058] The preparation of ginseng nanoemulsion includes: The nanoemulsion containing ginseng tissue culture provided in this embodiment comprises, by weight, 0.5 parts ginseng tissue culture, 3 parts butylene glycol, 1 part squalane, 0.5 parts polysorbate-20, 0.5 parts p-hydroxyacetophenone, and the remainder being purified water. The specific preparation method is as follows: Weigh out squalane, polysorbate-20 and butanediol, and stir mechanically until they are completely mixed. Heat the mixture to 70-75℃ and set aside. Weigh out purified water, p-hydroxyacetophenone, and ginseng tissue culture solution, stir well, and heat to 70-75℃. The concentration of the ginseng tissue culture solution is 2.8%, which means that the amount added is 1.5 parts based on pure ginseng tissue culture. The actual amount of ginseng tissue culture solution added is 17.86 parts.

[0059] The aqueous phase is added to the oil phase under heat preservation and rapid stirring, and the mixture is stirred evenly. The heat preservation temperature is 70-75℃. During the process of adding the aqueous phase to the oil phase, the addition method is slow at first and then fast. The first 1 / 3 of the aqueous phase material is added at a rate of 0.1L / min. After stirring evenly, the remaining aqueous phase is directly added to the oil phase. Then, the mixture is homogenized at normal pressure for 3 minutes to obtain a crude emulsion. The stirring speed is 300r / min; the homogenization speed at normal pressure is 2000r / min; and the homogenization temperature at normal pressure is 70-75℃.

[0060] The crude emulsion was cooled to below 45°C and subjected to high-pressure homogenization cycles 5 times at a homogenization pressure of 80 MPa to obtain ginseng tissue culture nanoemulsion.

[0061] The rest is the same as in Example 1, and will not be described in detail.

[0062] Example 3: The difference from Example 1 is that in this example, the mask essence is composed of the following ingredients: 3 parts ginseng tissue culture nanoemulsion, 15 parts polyol (all butylene glycol), 0.3 parts oil (including 0.1 parts meadowfoam seed oil, 0.05 parts olive fruit oil, 0.05 parts sunflower seed oil, 0.05 parts jojoba seed oil, and 0.05 parts grape seed oil), 0.5 parts black truffle extract, 0.01 parts complex anti-wrinkle peptides (including acetyl hexapeptide-8, acetyl tetrapeptide-7, and acetyl tetrapeptide-9 in a mass ratio of 1:1:1), 0.2 parts dipotassium glycyrrhizate, 0.15 parts hydrolyzed sodium hyaluronate, 1 part p-hydroxyacetophenone, 1 part 1,2-hexanediol, 0.5 parts hydroxyethyl cellulose, 0.5 parts PEG-40 hydrogenated castor oil, and the remainder is purified water, for a total weight of 100 parts. In the preparation of the ginseng nanofiber membrane: the inorganic nanocarbon material is a mixture of carbon black and bamboo charcoal in a mass ratio of 1:1. The particle size requirement for both carbon black and bamboo charcoal is D90 ≤ 10 micrometers. The remaining composition and preparation process of the ginseng nanofiber membrane are the same as in Example 1.

[0063] The preparation of ginseng nanoemulsion includes: The nanoemulsion containing ginseng tissue culture provided in this embodiment comprises, by weight, 1.5 parts ginseng tissue culture, 5 parts butylene glycol, 3 parts squalane, 1.5 parts polysorbate-20, 0.5 parts p-hydroxyacetophenone, and the remainder being purified water. The specific preparation method is as follows: Weigh out squalane, polysorbate-20 and butanediol, and mix them thoroughly by mechanical stirring. Heat the mixture to 65-70℃ for later use. Weigh out purified water, p-hydroxyacetophenone, and ginseng tissue culture solution, stir well, and heat to 65-70℃. The concentration of the ginseng tissue culture solution is 2.8%, which means that the amount added is 1.5 parts based on pure ginseng tissue culture. The actual amount of ginseng tissue culture solution added is 53.57 parts.

[0064] The aqueous phase is added to the oil phase under heat preservation and rapid stirring, and the mixture is stirred evenly. The heat preservation temperature is 65-70℃. During the process of adding the aqueous phase to the oil phase, the addition method is slow at first and then fast. The first 1 / 3 of the aqueous phase material is added at a rate of 0.1L / min. After stirring evenly, the remaining aqueous phase is directly added to the oil phase. Then, the mixture is homogenized at atmospheric pressure for 10 minutes to obtain a crude emulsion. The stirring speed is 500r / min; the homogenization speed at atmospheric pressure is 4000r / min; and the homogenization temperature at atmospheric pressure is 65-70℃.

[0065] The crude emulsion was cooled to below 45°C and subjected to high-pressure homogenization cycles 5 times at a homogenization pressure of 80 MPa to obtain ginseng tissue culture nanoemulsion.

[0066] The rest is the same as in Example 1, and will not be described in detail.

[0067] Comparative Example 1: The difference between Comparative Example 1 and Example 1 is that the same concentration of ginseng tissue culture was used instead of ginseng tissue culture nanoemulsion in the mask essence prepared in this comparative example.

[0068] In Example 1, 1.5 parts of ginseng tissue culture nanoemulsion were included. The amount of ginseng tissue culture nanoemulsion added was 1 part based on pure ginseng tissue culture, and 0.015 parts of ginseng tissue culture were actually added directly. Therefore, in Comparative Example 1, 0.54 parts of ginseng tissue culture solution with a concentration of 2.8% were added to replace ginseng tissue culture nanoemulsion as the raw material of the mask essence, and the remainder was made up with purified water.

[0069] Comparative Example 2: Ordinary Tencel facial mask fabric was used instead of ginseng nanofiber facial mask fabric, and all other aspects were the same as in Example 1.

[0070] Comparative Example 3: A mask sheet made of ordinary ginseng fiber and Tencel fiber blend was used instead of the ginseng nanofiber mask sheet. The mass ratio of ginseng fiber to Tencel fiber in the mask sheet raw material was 1:1, and the rest was the same as in Example 1.

[0071] Table 1. Components of the mask essence in Examples 1-3 and Comparative Examples 1-3

[0072] Table 2. Components of ginseng tissue culture nanoemulsions from Examples 1-3 and Comparative Examples 1-3

[0073] Experimental Results Example 1: Test Experiment on the Firming and Anti-wrinkle Effect of Facial Mask Sixty healthy participants aged 25-55 were selected and divided into six groups. After training by researchers, they underwent a trial. All participants had no history of skin diseases and were required to use the products provided by the researchers during the trial period, without applying any medications or cosmetics unrelated to the experiment. Before product use, the participants cleansed their faces with the same facial cleanser, dried their faces, and used a VISIA analyzer to photograph and analyze the initial values ​​of skin texture and wrinkles. Simultaneously, a skin elasticity tester was used to measure the initial value of skin elasticity R2. Then, the participants used the prescribed facial mask product once daily, and followed their personal skincare routine morning and evening with the researchers-provided moisturizing toner, lotion, and cream, which only had moisturizing effects. After 7 and 14 days of use, the participants cleansed their faces again with the facial cleanser, dried their faces, and again used the VISIA analyzer and skin elasticity tester for analysis. Comparative analysis revealed changes in skin texture, wrinkles, and the total skin elasticity parameter R2 before and after mask use.

[0074] Groups 1-6 of participants selected and tested the facial mask products obtained in Examples 1, 2, and 3, and Comparative Examples 1, 2, and 3, respectively. All experimental data in this experiment were obtained under the same conditions. The number of skin texture and wrinkle features was measured by taking pictures and analyzing them with the VISIA testing instrument. The smaller the value, the better, indicating that the skin surface is smoother and more delicate. The total elasticity parameter R2 value obtained by the skin elasticity test was also tested. The larger the value, the better. The closer the value is to 1, the better the skin elasticity.

[0075] The results of the human experience efficacy test are summarized in Table 3. The values ​​of each item are the average changes of the 10 participants in each group.

[0076] Table 3 Summary of Human Experience Efficacy Test Results Analysis

[0077] According to the test results in Table 3, the improvement in facial texture, wrinkles, and skin elasticity increased with the increase in the amount of ginseng tissue culture nanoemulsion used. Furthermore, under the same experimental conditions, the skin improvement effects of the ginseng tissue culture nanoemulsion group were significantly better than those of the non-nanoemulsion group. Additionally, the ginseng nanofiber membrane was significantly superior to both the non-nano ginseng fiber mask and ordinary fiber mask. This indicates that the ginseng tissue culture nanoemulsion and ginseng nanofiber membrane work synergistically to improve the transdermal absorption and bioavailability of the active ingredients, resulting in better firming and anti-wrinkle effects for the mask product.

[0078] Experimental Results Example 2: In vitro efficacy test of the mask essence to assess its firming, anti-wrinkle, and soothing effects. The mask of Example 1 of this invention was sent to a third party for external testing of the elastase inhibition rate, collagenase inhibition rate and hyaluronidase inhibition rate of the mask essence liquid.

[0079] Experimental Methods: Samples of the facial mask essence were mixed with elastase, collagenase, and hyaluronidase, respectively, and incubated at 37°C for 10 min. NSAAAp solution was then added, and the mixture was enzymatically hydrolyzed at 37°C for 40 min. The absorbance was measured using a UV spectrophotometer for analysis. The inhibition rates of elastase, collagenase, and hyaluronic acid in the essence were obtained and compared with a blank control. The results are shown in Table 4.

[0080] Table 4. Statistics on the inhibition rates of elastase, collagenase, and hyaluronic acid in the essence emulsion.

[0081] The above tests prove that the facial mask essence of this invention has very significant firming, anti-wrinkle, and soothing effects.

[0082] The ginseng tissue culture nanoemulsion obtained in Example 1 and the non-nanoemulsion with the same concentration of ginseng tissue culture were sent to a third party for human transdermal absorption Raman spectroscopy detection experiments. The results showed that the nanoemulsion was superior to the non-nanoemulsion in terms of penetration depth, transdermal absorption rate and penetration amount. The preparation steps of the non-nanoemulsion were exactly the same as those in Example 1 in steps S101-S103, except for step S104. The test results are shown in Table 5.

[0083] Table 5. Transdermal absorption test results of nanoemulsions and non-nanoemulsions

[0084] The encapsulation efficiency was tested using the method provided in section 2.2.3, "Study on the Stability and Bioavailability of Whey Protein-Based Ginsenoside Rg3 Nanoemulsion," from the Jilin University paper "[1] Hou Pingping. Study on the Stability and Bioavailability of Whey Protein-Based Ginsenoside Rg3 Nanoemulsion [D]. Jilin University, 2020. DOI:10.27162 / d.cnki.gjlin.2020.006329.". Specifically, the encapsulation efficiency was determined using the method provided in section 2.2.3, "Determination of Nanoemulsion Encapsulation Efficiency." Extraction of ginsenosides from nanoemulsions: Take 1 mL of the nanoemulsion containing ginseng tissue culture provided in this application, mix it with 4 mL of methanol, sonicate for 10 min, and then place it in a centrifuge. Set the speed to 10000 rpm and centrifuge for 30 min. After centrifugation, take out the supernatant as the sample to be tested.

[0085] Take the ginseng tissue culture solution corresponding to each example, and add purified water to make up the remaining weight (excluding the ginseng tissue culture) to 100 parts, then mix well as a control sample. Taking Example 1 as an example, Example 1 includes 1 part of ginseng tissue culture, and the actual amount of ginseng tissue culture solution added is 35.71 parts. Add 64.29 parts of purified water to the 35.71 parts of ginseng tissue culture solution and mix well (total weight is 100 parts). Take 1 mL of the mixture and mix it with 4 mL of methanol as a control sample.

[0086] Chromatographic conditions: C18 column (250×4.6mm, inner diameter 5μm), mobile phase acetonitrile:water = 45:55 (v / v), detector wavelength 203nm, mobile phase flow rate 1.0mL / min, injection volume 10μL, column temperature 25℃.

[0087] Encapsulation efficiency is calculated using the following formula: ; In the formula, Sto be tested is the total peak area of ​​ginsenoside Rg3, ginsenoside Rk1, ginsenoside Rg5 and ginsenoside Rh2 in the test sample, and Stotal is the total peak area of ​​ginsenoside Rg3, ginsenoside Rk1, ginsenoside Rg5 and ginsenoside Rh2 in the control sample.

[0088] The liquid chromatography test results of the test sample box and the control sample in Example 1 are as follows: Figure 4 and Figure 5 As shown, the encapsulation rate of ginsenosides in Example 1 was calculated to be 83.25%.

[0089] The ginseng tissue culture nanoemulsion obtained in Example 1 was placed at 25℃ and 40℃ / 75%RH for 90 days, respectively. The particle size distribution of the ginseng tissue culture and its aqueous solution after 90 days was tested, and the results are shown in Table 6. Figure 1 , 2 As shown in Figure 3.

[0090] Table 6. Particle size distribution results after stability testing of nanoemulsions

[0091] Zebrafish raw material efficacy test: The ginseng tissue culture nanoemulsion obtained in Example 1 and the non-nanoemulsion with the same ratio were used to test the efficacy of zebrafish in scavenging reactive oxygen species, promoting collagen gene expression and promoting elastin gene expression.

[0092] The test method for Zebrafish ROS scavenging is as follows: Healthy zebrafish embryos, 2 days after fertilization, were selected for testing. A blank control group (fish embryo culture medium), a positive control group (glutathione working solution), and a test substance group (test substance) were set up.

[0093] Twenty-four 2-day-old zebrafish embryos were randomly selected from each group, and 0.2 mL of the corresponding solution was allocated to each embryo. After incubation at a constant temperature for 24 hours, the embryos were transferred. After transfer, 2 mL of reactive oxygen species (ROS) test solution was allocated to each 12 embryos, and the embryos were incubated at a constant temperature for another 2 hours.

[0094] The fish embryos were placed sideways. Images of the embryos were taken under a fluorescence stereomicroscope using standardized parameters. The images were opened with analysis software, and the yolk sac region of each embryo was marked. Then, the "Measurement" tab was selected, and the "Average Intensity" was chosen as the ROS signal intensity. The reactive oxygen species (ROS) scavenging rate was calculated. Clearance rate = (BS) / B × 100%; In the formula, S represents the average ROS signal intensity of fish embryos in the test substance treatment group; B represents the average ROS signal intensity of fish embryos in the blank control group.

[0095] The method for testing the expression promotion rate of zebrafish type I collagen genes is as follows: A blank control group (fish embryo culture medium), a positive control group (acetyl hexapeptide-8), and a test substance group (test substance) were set up. Thirty-six zebrafish were randomly selected from each group, and 2.5 mL of the corresponding solution was allocated to every 12 zebrafish. After 24 hours of incubation at a constant temperature, 12 zebrafish from each well were collected into 1.5 mL tubes, the solution was removed, 0.5 mL of RNAlater solution was added, and the tubes were frozen for storage. RNA extraction, cDNA synthesis, and real-time RT-PCR were then performed.

[0096] RNA extraction included: removing RNA later solution and rinsing three times with PBS. The embryos were placed on ice, the PBS solution was removed, and 500 μL of TRIzol reagent was added. The embryos were homogenized using a pellet mill and placed on ice for 10 min. 100 μL of chloroform was added, the mixture was vortexed for 1 min, and placed on ice for 5 min. The sample was centrifuged for 20 min, the supernatant was transferred to a 1.5 mL centrifuge tube, 250 μL of isopropanol was added, the mixture was vortexed for 1 min, placed on ice for 10 min, and centrifuged for 20 min. All supernatant was removed, 500 μL of ethanol was added, the mixture was vortexed for 1 min, centrifuged for 5 min, and placed on ice for 10 min. This step was repeated three times. The ethanol was removed, the sample was centrifuged for 5 min, and the sample was air-dried in a fume hood with the lid open for 10 min. 10 μL of DNase / RNase-free ultrapure distilled water was added, and the sample was heated at 55°C for 15 min.

[0097] cDNA synthesis involved diluting the RNA sample to 1000 ng / 7 μL with DNase / RNase-free ultrapure distilled water. The RNA was then synthesized into cDNA using a PrimeScript RT kit containing a gDNA Eraser (Takara; Cat no. RR047A), and stored frozen.

[0098] Real-time RT-PCR includes: Prepare primer mixtures and real-time PCR master mixtures for each primer. Dilute cDNA samples 10-fold with DNase / RNase-free ultrapure distilled water. Add 9 μL of PCR master mixture and 1 μL of diluted cDNA sample to each well of a 96-well PCR plate. Seal the 96-well PCR plate with optical sealant, centrifuge the plate for 5 min, and perform real-time PCR amplification.

[0099] Real-time PCR data were then collected. Ct was used as the amplification result, and the amplification amount of the β-actin gene was used as the housekeeping gene. The relative expression levels of each gene (col1a1a, col1a1b) were calculated as the test result, and the gene expression promotion rate was calculated according to the following formula: ; ; ; ; In the formula, This represents the difference between the cycle number of the target gene and the cycle number of the housekeeping gene. This indicates the number of cycles required for the target gene fluorescence signal to reach a set threshold. This indicates the number of cycles required for the housekeeper gene fluorescence signal to reach a set threshold. Indicates blank control group Average value and test group difference, This represents the average difference between the cycle number of the target gene and the cycle number of the housekeeping gene in the blank control group. This represents the difference between the cycle number of the target gene and the cycle number of the housekeeping gene in the test group. This represents the relative expression level of the target gene in the blank control group. This indicates the relative expression level of the target gene in the test group.

[0100] The results of the comparative efficacy test between ginseng tissue culture nanoemulsion and non-nanoemulsion are shown in Table 7: Table 7. Comparative Test Results of Efficacy of Ginseng Tissue Culture Nanoemulsion and Non-Nanoemulsion

[0101] Negative ion performance test: The fibers prepared in Examples 1-7 and Comparative Example 1 were spun into 60S yarn and woven into double-sided knitted fabrics. The fabrics were tested according to GB / T30128-2013 "Detection and Evaluation of Negative Ion Generation in Textiles". GB / T30128-2013 evaluates negative ion generation as follows: >1000 ions indicate high negative ion generation; 550-1000 ions indicate medium negative ion generation; <550 ions indicate low negative ion generation. The test results are shown in Table 8. Table 8. Negative ion performance test results of Examples 1-7 and Comparative Example 1

[0102] The above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application, and should all be included within the protection scope of this application.

Claims

1. A firming and anti-wrinkle facial mask containing ginseng tissue culture nanoemulsion, comprising a mask sheet and a mask essence, characterized in that: The mask essence contains the following components in parts by weight: The composition consists of 0.5-3 parts ginseng tissue culture nanoemulsion, 5-15 parts polyol, 0.1-0.3 parts oil, 0.05-0.5 parts black truffle extract, 0.001-0.01 parts anti-wrinkle peptide, 0.05-0.2 parts dipotassium glycyrrhizate, 0.05-0.15 parts hydrolyzed sodium hyaluronate, 0.1-1 parts p-hydroxyacetophenone, 0.1-1 parts 1,2-hexanediol, 0.1-0.5 parts hydroxyethyl cellulose, 0.2-0.5 parts PEG-40 hydrogenated castor oil, and the remainder being purified water, for a total weight of 100 parts.

2. The firming and anti-wrinkle facial mask containing ginseng tissue culture nanoemulsion according to claim 1, characterized in that: The ginseng tissue culture nanoemulsion comprises, by weight: 0.5-1.5 parts ginseng tissue culture, 3-5 parts butylene glycol, 1-3 parts squalane, 0.5-1.5 parts polysorbate-20, 0.5 parts p-hydroxyacetophenone, and the balance being purified water, for a total weight of 100 parts; the average particle size of the nanoemulsion is 100-200 nm, and the particle size distribution coefficient of the nanoemulsion, expressed as a polydispersity index, is less than 0.

2.

3. The firming and anti-wrinkle facial mask containing ginseng tissue culture nanoemulsion according to claim 2, characterized in that: The ginseng tissue culture is added in the form of a ginseng tissue culture solution, and the weight of the ginseng tissue culture in the nanoemulsion is calculated based on the actual content of the ginseng tissue culture in the ginseng tissue culture solution. The preparation of ginseng tissue culture solution includes: freezing ginseng tissue culture, thawing it, adding water to homogenize it, adjusting the pH of the solution; adding enzymes while controlling the temperature, performing enzymatic hydrolysis, inactivating the enzymes after enzymatic hydrolysis, centrifuging, and reserving the supernatant; Water was added to the precipitate after centrifugation, and the mixture was refluxed for extraction. After filtration, ethanol was added to the filtrate and allowed to stand. The precipitate was then combined with the supernatant to obtain a ginseng tissue culture solution.

4. The firming and anti-wrinkle facial mask containing ginseng tissue culture nanoemulsion according to claim 3, characterized in that: The preparation process of the ginseng tissue culture nanoemulsion includes the following steps: S101: Weigh out squalane, polysorbate-20 and butanediol, stir and mix them evenly, then heat to 65-75℃ for later use to obtain the oil phase; S102: Weigh purified water, p-hydroxyacetophenone and ginseng tissue culture solution, stir and mix evenly, then heat to 65-75℃ for later use to obtain the aqueous phase; S103: Add the aqueous phase to the oil phase under heat preservation and stirring. The heat preservation temperature is 65-75℃. After the addition is completed, homogenize for 3-10 minutes to obtain a crude emulsion. The stirring speed is 300-500 r / min. The homogenization speed is 2000-4000 r / min and the temperature is 65-75℃. S104: Cool the crude emulsion to below 45℃, homogenize it under high pressure for 5 cycles, and homogenize it at a pressure of 80MPa to obtain ginseng tissue culture nanoemulsion.

5. The firming and anti-wrinkle facial mask containing ginseng tissue culture nanoemulsion according to claim 1, characterized in that: The mask fabric is a ginseng nanofiber mask fabric. The preparation of the ginseng nanofiber mask fabric includes: chemically modifying inorganic nano-carbon materials to obtain a modified inorganic nano-carbon material slurry; adding a dispersant to the modified inorganic nano-carbon material slurry for dispersion to obtain an inorganic nano-carbon dispersion; preparing a ginsenoside solution; adding the ginsenoside solution to the inorganic nano-carbon dispersion for dispersion to obtain an inorganic-organic mixed dispersion; mixing the inorganic-organic mixed dispersion with a cellulose spinning solution for the first time to obtain a composite dispersion; mixing the composite dispersion with the cellulose spinning solution for the second time to obtain a composite spinning adhesive; spinning the composite spinning adhesive using wet spinning to obtain a highly responsive fiber with synergistic structure-activity relationship between inorganic and organic materials; and finally, spinning the fiber into yarn and weaving it into a ginseng nanofiber fabric using a double-sided circular knitting machine.

6. The firming and anti-wrinkle facial mask containing ginseng tissue culture nanoemulsion according to claim 5, characterized in that: The inorganic nano-carbon material is one or more of fullerene, graphene, carbon nanotubes, carbon black, and bamboo charcoal. The cellulose spinning solution is modal fiber spinning solution. In the first mixing, the mass fraction ratio of ginseng multifunctional powder to cellulose spinning solution is 3:

7.

7. The firming and anti-wrinkle facial mask containing ginseng tissue culture nanoemulsion according to claim 1, characterized in that: The polyol is one or a mixture of butanediol, glycerol, methyl propylene glycol, and polyethylene glycol-8.

8. The firming and anti-wrinkle facial mask containing ginseng tissue culture nanoemulsion according to claim 1, characterized in that: The oil is one or more of meadowfoam seed oil, olive fruit oil, sunflower seed oil, jojoba seed oil, and grape seed oil, or a mixture thereof.

9. The firming and anti-wrinkle facial mask containing ginseng tissue culture nanoemulsion according to claim 1, characterized in that: The anti-wrinkle polypeptide is one or more of the following: tripeptide-1, palmitoyl pentapeptide-4, hexapeptide-9, arginine / lysine polypeptide, carnosine, acetyl hexapeptide-8, acetyl tetrapeptide-7, and acetyl tetrapeptide-9.

10. A preparation process for a firming and anti-wrinkle facial mask containing ginseng tissue culture nanoemulsion, characterized in that, The preparation of a firming and anti-wrinkle facial mask containing ginseng tissue culture nanoemulsion as described in claims 1-9 includes the following steps: S201: Add polyol, hydroxyethyl cellulose, and hydrolyzed sodium hyaluronate to 75 parts of purified water, stir to disperse evenly, and simultaneously heat to 75-80℃. Stir until completely dissolved, then cool down. S202: Cool to 55-60℃, add p-hydroxyacetophenone and 1,2-hexanediol, stir to dissolve, and continue cooling; S203: When the temperature drops to below 40℃, add dipotassium glycyrrhizate, oil, PEG-40 hydrogenated castor oil, anti-wrinkle peptides, black truffle extract, and ginseng tissue culture nanoemulsion while stirring. Stir until completely dispersed and evenly mixed. Use purified water to make up the total weight to 100 parts. Cool to below 35℃ to obtain the mask essence. S204: Fill the mask essence and ginseng nanofiber mask cloth into a mask bag and seal it to make a sheet mask.

Images