Method for separating inorganic selenium from selenoprotein primary product
By combining precipitation, ultrafiltration, reverse osmosis and dialysis techniques, inorganic selenium is separated from selenoproteins, solving the problem of high inorganic selenium content and improving the purity and bioactivity of organic selenium. This method is suitable for direct consumption in food, pharmaceuticals, and health products, as well as for industrial applications.
Patent Information
- Application Number
- CN202411091222.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-09
- Publication Date
- 2026-02-10
AI Technical Summary
Existing selenoprotein products have a high content of inorganic selenium, resulting in low biological activity and side effects, making it difficult to achieve high-purity organic selenium separation.
A technical solution combining precipitation, ultrafiltration, reverse osmosis and dialysis is used to separate inorganic selenium from selenoproteins. Through multiple precipitation and dilution steps, a high-purity organic selenium product is obtained.
It improves the purity and bioactivity of organic selenium, reduces side effects, and purifies selenoproteins, making them suitable for direct consumption in food, pharmaceuticals, and health products, as well as for industrial applications.
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Figure CN121493878A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of selenoprotein technology, specifically a method for separating inorganic selenium from primary selenoprotein products. Background Technology
[0002] Selenium is a nonmetallic element in Group 16 of the fourth period of the periodic table. Its element symbol is Se, its atomic number is 34, and its relative atomic mass is 78.96.
[0003] Selenium is highly toxic, but it can conduct electricity and heat.
[0004] On Earth, selenium is divided into inorganic selenium and organic selenium.
[0005] Inorganic selenium includes sodium selenate, sodium selenite, and inorganic selenides, which exist in the form of inorganic salts. Inorganic selenium is a compound formed by the combination of selenium with inorganic substances, including inorganic selenides, selenates, and selenites. These compounds are widely found in nature, but they generally have varying degrees of toxicity, some even being highly toxic. Inorganic selenium has poor biological activity and low bioavailability; therefore, special attention must be paid to its safety and effectiveness when using it.
[0006] On October 27, 2017, the International Agency for Research on Cancer of the World Health Organization published a preliminary list of carcinogens, which included selenium and selenium compounds in Group 3.
[0007] In the People's Republic of China National Standard for Drinking Water Quality (GB 5749), selenium is listed as a mandatory toxicological indicator, with a limit of ≤10ug / l.
[0008] Selenium (organic selenium) – suitable for (activating) the cellular activities of vital molecules.
[0009] Selenium (organic selenium) exists in the form of selenoamino acids and selenoproteins.
[0010] Selenium (organic selenium) is an essential element for life.
[0011] Compared to inorganic selenium, organic selenium has higher bioactivity and bioavailability, making it a more ideal form of selenium supplementation.
[0012] Selenium is an essential trace element for humans. In 1973, the World Health Organization (WHO) confirmed that selenium is an essential trace element for human and animal life. It is a trace element that the human body cannot synthesize and must be obtained from external sources.
[0013] Selenium is present in human tissues in a fraction of one part per ten million, yet it is essential for the existence of life and its vital role in human health is irreplaceable by any other substance.
[0014] Selenium deficiency can directly lead to a decline in the human immune system.
[0015] The absorption and utilization of selenium largely depend on its form, playing a crucial role in bioavailability and effectiveness. Distinguishing between total selenomethionine and bioavailable selenomethionine is also important, as only free selenoamino acids can replace methionine and participate non-specifically in protein synthesis. Whether it's total selenium or total selenomethionine, the body can only directly utilize it after the selenium source has been digested into free selenomethionine.
[0016] In other words, only pure organic selenium can be digested and utilized by the human body. Inorganic selenium can only be digested, utilized, or organicated by other living organisms besides humans.
[0017] To date, most selenoprotein / organic selenium products are a mixture of organic and inorganic selenium, making them primary products. For example, commercial selenium yeast / selenium-enriched yeast products generally have less than 80% organic selenium and more than 20% inorganic selenium. Therefore, they can cause significant side effects on the human body, such as hair loss, digestive disorders, general weakness, nail deformities, and liver and kidney metabolic damage. In severe cases, chronic selenium poisoning may occur. Although DYG selenoprotein, selenium mushroom powder, selenium shepherd's purse powder, and selenium algae powder have an organic selenium content of up to 90%, they are still in the primary product stage, with about 10% inorganic selenium remaining in the selenoprotein, negatively impacting the body and limiting their consumption.
[0018] Strictly speaking, selenoproteins / organic selenium must have an organic selenium content of over 95% to be considered selenoproteins / organic selenium and to be suitable for human consumption. Summary of the Invention
[0019] To address the shortcomings of existing inorganic selenium in selenoproteins / organic selenium.
[0020] The purpose of this invention is to solve the technical problem of separating inorganic selenium from existing selenoproteins, making the selenoproteins purer, with a higher organic selenium content (99.99%), improving the biological activity of selenium, making it more conducive to the absorption and utilization of living organisms, and reducing its side effects.
[0021] Based on the characteristics of inorganic selenium having a higher density and higher conductivity than organic selenium, this technical solution adopts a combination of precipitation, ultrafiltration and dialysis to separate the inorganic selenium present in selenoproteins, thereby obtaining a purer selenoprotein product.
[0022] Technical solution of the present invention A method for separating inorganic selenium from selenoprotein primary products, characterized by the following steps: Step 1: Selenium protein powder / selenium yeast powder / selenium mushroom powder / selenium shepherd's purse powder / selenium algae powder into the selenium protein raw material preparation tank (1) Add pure water, adjust the pH value of the solution to alkaline, ozone treatment / no ozone treatment, heating / no heating; Step 2: Centrifuge the selenoprotein solution described in Step 1 in a centrifuge (2) at low temperature to obtain the selenoprotein solution; Step 3: Place the selenoprotein solution described in Step 2 into the selenoprotein solution precipitation processor (3) for natural precipitation / physical precipitation / chemical precipitation, 2H-24H, to separate the selenoprotein supernatant and selenoprotein sub-concentrate; Step 4: Take out the selenoprotein supernatant from step 3 and place it in the selenoprotein supernatant storage container (4) to obtain the selenoprotein subconcentrate slurry; Step 5: Dilute the selenoprotein sub-concentrated slurry described in Step 4 with pure water, precipitate, take the supernatant, and place the secondary selenoprotein supernatant in the selenoprotein supernatant storage container (4) to obtain the secondary selenoprotein sub-concentrated slurry. Step 6: Dilute the secondary selenoprotein slurry obtained in Step 5 with pure water, precipitate, take the supernatant, and place the tertiary selenoprotein supernatant in the selenoprotein supernatant storage container (4) to obtain the tertiary selenoprotein slurry. Step 7: The first, second, and third selenoprotein supernatants described in steps 1 to 6 are sterilized, packaged / concentrated, and dried in a sterilization-concentration-drying device (6). The concentration is preferably carried out by reverse osmosis to separate the selenoprotein supernatant to obtain selenoprotein slurry and purified water. The drying is carried out by freeze-drying the selenoprotein slurry to obtain high-purity (Grade 1 - 99.99%) selenoprotein liquid / selenoprotein powder, which can be directly consumed and used in food, medicine, health products, and nutritional supplements. The purified water can be reused in production. Step 8: Dilute the concentrated slurry of selenoprotein obtained in Step 5 with pure water, and use ultrafiltration to separate it into clear selenoprotein and turbid selenoprotein. Step 9: The selenoprotein turbid liquid described in Step 8 is preferably fed into a reverse osmosis and dialysis device (5) for separation by reverse osmosis to obtain selenoprotein (2nd grade) slurry and pure water. The selenoprotein (2nd grade) slurry is preferably freeze-dried to obtain selenoprotein (2nd grade - 98%) powder. The obtained pure water is recycled for production. Step 10: The selenoprotein solution described in Step 8 is preferably fed into a reverse osmosis and dialysis device (5) and dialysis is used to separate it into selenoprotein (3-stage) solution and inorganic selenium solution. Step 11: The selenoprotein (grade 3) solution described in step 10 is preferably fed into a reverse osmosis and dialysis device (5) for separation by reverse osmosis to obtain selenoprotein (grade 3) concentrate and purified water. The selenoprotein (grade 3) concentrate is preferably freeze-dried to obtain selenoprotein (grade 3-95%) powder. The purified water obtained is recycled for production. Step 12: The inorganic selenium solution described in Step 10 is preferably fed into a reverse osmosis and dialysis device (5) for separation by reverse osmosis to obtain inorganic selenium concentrate and pure water. The inorganic selenium concentrate is dried to obtain inorganic selenium powder, which is recycled for selenium organic / industrial use, and the pure water is recycled for production.
[0023] In step one, it is preferable to adjust the pH of the selenoprotein solution to 7.8-8.8.
[0024] Steps four and five are preferably repeated three times, but can be repeated multiple times if necessary.
[0025] In step one, it is preferable to treat the selenium yeast selenoprotein solution with ozone at a volume ratio of 5%-6% for 15-30 minutes, while simultaneously heating it to 60℃-70℃, letting it stand for 15-30 minutes, and then cooling it to room temperature.
[0026] In step two, centrifugation at a low temperature of 2℃-4℃ is preferred.
[0027] Steps one through twelve are preferably configured as a pure selenoprotein and inorganic selenium separation system device. Attached Figure Description
[0028] The accompanying drawings, which form part of this specification, are provided to further illustrate embodiments of the invention and, together with the textual description, explain the principles of the invention. It is obvious that the drawings described below are merely some embodiments of the invention, and those skilled in the art can obtain other drawings based on these drawings without any creative effort.
[0029] Figure 1 A schematic diagram of a single-unit pure selenoprotein and inorganic selenium separation system device according to a specific embodiment of the present invention is shown; The above figures include the following reference numerals: 1. Selenium protein raw material preparation tank; 2. Centrifuge; 3. Selenium protein solution precipitation processor; 4. Selenium protein supernatant storage tank; 5. Reverse osmosis and dialysis device; 6. Sterilization-concentration-drying treatment device.
[0030] The beneficial effects of the present invention: The present invention discloses a method for separating inorganic selenium from primary selenoprotein products.
[0031] The technical problem of separating inorganic selenium from existing selenoproteins has been solved, making the selenoproteins purer and with a higher organic selenium content (99.99%), improving the bioactivity and effectiveness of selenium, making it more conducive to the absorption and utilization of living organisms, and reducing its side effects.
[0032] Based on the characteristics of inorganic selenium having a higher density and higher conductivity than organic selenium, the technical solution of this invention adopts a combination of precipitation, ultrafiltration, reverse osmosis and dialysis to separate the inorganic selenium present in selenoproteins, thereby obtaining a purer selenoprotein product.
[0033] After being separated by the process steps of this invention, organic selenium primary products such as selenium protein powder, selenium yeast powder, selenium mushroom powder and selenium algae powder are obtained as highly pure (95%-99.99%) selenium protein, inorganic selenium and purified water.
[0034] Prioritizing environmental protection requirements, the separation process achieves zero emissions, making it extremely environmentally friendly.
[0035] The advent of pure selenoproteins has made selenium supplementation no longer restricted, making it possible for ordinary people to access selenium supplementation.
[0036] The present invention will be further described below with reference to the embodiments. Detailed Implementation
[0037] Example: A method for separating inorganic selenium from selenoprotein primary products, characterized by the following steps: Step 1: Selenium protein powder / selenium yeast powder / selenium mushroom powder / selenium shepherd's purse powder / selenium algae powder into the selenium protein raw material preparation tank (1) Add pure water, adjust the pH value of the solution to alkaline, ozone treatment / no ozone treatment, heating / no heating; Step 2: Centrifuge the selenoprotein solution described in Step 1 in a centrifuge (2) at low temperature to obtain the selenoprotein solution; Step 3: Place the selenoprotein solution described in Step 2 into the selenoprotein solution precipitation processor (3) for natural precipitation / physical precipitation / chemical precipitation, 2H-24H, to separate the selenoprotein supernatant and selenoprotein sub-concentrate; Step 4: Take out the selenoprotein supernatant from step 3 and place it in the selenoprotein supernatant storage container (4) to obtain the selenoprotein subconcentrate slurry; Step 5: Dilute the selenoprotein sub-concentrated slurry described in Step 4 with pure water, precipitate, take the supernatant, and place the secondary selenoprotein supernatant in the selenoprotein supernatant storage container (4) to obtain the secondary selenoprotein sub-concentrated slurry. Step 6: Dilute the secondary selenoprotein slurry obtained in Step 5 with pure water, precipitate, take the supernatant, and place the tertiary selenoprotein supernatant in the selenoprotein supernatant storage container (4) to obtain the tertiary selenoprotein slurry. Step 7: The first, second, and third selenoprotein supernatants described in steps 1 to 6 are sterilized, packaged / concentrated, and dried in a sterilization-concentration-drying device (6). The concentration is preferably carried out by reverse osmosis to separate the selenoprotein supernatant to obtain selenoprotein slurry and purified water. The drying is carried out by freeze-drying the selenoprotein slurry to obtain high-purity (Grade 1 - 99.99%) selenoprotein liquid / selenoprotein powder, which can be directly consumed and used in food, medicine, health products, and nutritional supplements. The purified water can be reused in production. Step 8: Dilute the concentrated slurry of selenoprotein obtained in Step 5 with pure water, and use ultrafiltration to separate it into clear selenoprotein and turbid selenoprotein. Step 9: The selenoprotein turbid liquid described in Step 8 is preferably fed into a reverse osmosis and dialysis device (5) for separation by reverse osmosis to obtain selenoprotein (2nd grade) slurry and pure water. The selenoprotein (2nd grade) slurry is preferably freeze-dried to obtain selenoprotein (2nd grade - 98%) powder. The obtained pure water is recycled for production. Step 10: The selenoprotein solution described in Step 8 is preferably fed into a reverse osmosis and dialysis device (5) and dialysis is used to separate it into selenoprotein (3-stage) solution and inorganic selenium solution. Step 11: The selenoprotein (grade 3) solution described in step 10 is preferably fed into a reverse osmosis and dialysis device (5) for separation by reverse osmosis to obtain selenoprotein (grade 3) concentrate and purified water. The selenoprotein (grade 3) concentrate is preferably freeze-dried to obtain selenoprotein (grade 3-95%) powder. The purified water obtained is recycled for production. Step 12: The inorganic selenium solution described in Step 10 is preferably fed into a reverse osmosis and dialysis device (5) for separation by reverse osmosis to obtain inorganic selenium concentrate and pure water. The inorganic selenium concentrate is dried to obtain inorganic selenium powder, which is recycled for selenium organic / industrial use, and the pure water is recycled for production.
[0038] In step one, it is preferable to adjust the pH of the selenoprotein solution to 7.8-8.8.
[0039] Steps four and five are preferably repeated three times, but can be repeated multiple times if necessary.
[0040] In step one, it is preferable to treat the selenium yeast selenoprotein solution with ozone at a volume ratio of 5%-6% for 15-30 minutes, while simultaneously heating it to 60℃-70℃, letting it stand for 15-30 minutes, and then cooling it to room temperature.
[0041] In step two, centrifugation at a low temperature of 2℃-4℃ is preferred.
[0042] Steps one through twelve are preferably configured as a pure selenoprotein and inorganic selenium separation system device.
Claims
1. A method for separating inorganic selenium from selenoprotein primary products, Its features include the following steps: Step 1: Selenium protein powder / selenium yeast powder / selenium mushroom powder / selenium shepherd's purse powder / selenium algae powder into the selenium protein raw material preparation tank (1) Add pure water, adjust the pH value of the solution to alkaline, ozone treatment / no ozone treatment, heating / no heating; Step 2: Centrifuge the selenoprotein solution described in Step 1 in a centrifuge (2) at low temperature to obtain the selenoprotein solution; Step 3: Place the selenoprotein solution described in Step 2 into the selenoprotein solution precipitation processor (3) for natural precipitation / physical precipitation / chemical precipitation, 2H-24H, to separate the selenoprotein supernatant and selenoprotein sub-concentrate; Step 4: Take out the selenoprotein supernatant from step 3 and place it in the selenoprotein supernatant storage container (4) to obtain the selenoprotein subconcentrate slurry; Step 5: Dilute the selenoprotein sub-concentrated slurry described in Step 4 with pure water, precipitate, take the supernatant, and place the secondary selenoprotein supernatant in the selenoprotein supernatant storage container (4) to obtain the secondary selenoprotein sub-concentrated slurry. Step 6: Dilute the secondary selenoprotein slurry obtained in Step 5 with pure water, precipitate, take the supernatant, and place the tertiary selenoprotein supernatant in the selenoprotein supernatant storage container (4) to obtain the tertiary selenoprotein slurry. Step 7: The first, second, and third selenoprotein supernatants described in steps 1 to 6 are sterilized, packaged / concentrated, and dried in a sterilization-concentration-drying device (6). The concentration is preferably carried out by reverse osmosis to separate the selenoprotein supernatant to obtain selenoprotein slurry and purified water. The drying is carried out by freeze-drying the selenoprotein slurry to obtain high-purity (Grade 1 - 99.99%) selenoprotein liquid / selenoprotein powder, which can be directly consumed and used in food, medicine, health products, and nutritional supplements. The purified water can be reused in production. Step 8: Dilute the concentrated slurry of selenoprotein obtained in Step 5 with pure water, and use ultrafiltration to separate it into clear selenoprotein and turbid selenoprotein. Step 9: The selenoprotein turbid liquid described in Step 8 is preferably fed into a reverse osmosis and dialysis device (5) for separation by reverse osmosis to obtain selenoprotein (2nd grade) slurry and pure water. The selenoprotein (2nd grade) slurry is preferably freeze-dried to obtain selenoprotein (2nd grade - 98%) powder. The obtained pure water is recycled for production. Step 10: The selenoprotein solution described in Step 8 is preferably fed into a reverse osmosis and dialysis device (5) and dialysis is used to separate it into selenoprotein (3-stage) solution and inorganic selenium solution. Step 11: The selenoprotein (grade 3) solution described in step 10 is preferably fed into a reverse osmosis and dialysis device (5) for separation by reverse osmosis to obtain selenoprotein (grade 3) concentrate and purified water. The selenoprotein (grade 3) concentrate is preferably freeze-dried to obtain selenoprotein (grade 3-95%) powder. The purified water obtained is recycled for production. Step 12: The inorganic selenium solution described in Step 10 is preferably fed into a reverse osmosis and dialysis device (5) for separation by reverse osmosis to obtain inorganic selenium concentrate and pure water. The inorganic selenium concentrate is dried to obtain inorganic selenium powder, which is recycled for selenium organic / industrial use, and the pure water is recycled for production.
2. In step one according to claim 1, it is preferable to adjust the pH value of the selenoprotein solution to 7.8-8.
8.
3. According to steps four and five of claim 1, it is preferable to repeat them three times, and if necessary, they can be repeated multiple times.
4. In step one according to claim 1, it is preferable to treat the selenium yeast selenoprotein solution with ozone at a volume ratio of 5%-6% for 15-30 minutes, while heating to 60℃-70℃, letting it stand for 15-30 minutes, and then cooling to room temperature.
5. In step two according to claim 1, it is preferred to use low-temperature centrifugation at 2℃-4℃.
6. The method for separating inorganic selenium from selenoprotein according to claims 1-5 is preferably configured as a pure selenoprotein and inorganic selenium separation system device.