Selenium-enriched yeast additive for relieving tear stains of pets

Abstract

The invention belongs to the technical field of functional feed additives for pets, and discloses a selenium-enriched yeast additive for relieving tear stains of pets, the additive takes selenium-enriched yeast as a core effective component, the content of organic selenium in the selenium-enriched yeast is not less than 1000 mg / kg, and the content of organic selenium in the selenium-enriched yeast is not less than 1000 mg / kg. The chrysanthemum extract, the honeysuckle flower extract and the semen cassiae extract are compounded to form an anti-inflammatory heat-clearing component which is used for relieving lacrimal gland inflammation reaction and reducing abnormal tear secretion; meanwhile, L-lysine, taurine, vitamin C and xanthophyll are introduced as nutrition synergistic components, so that the lacrimal gland mucosa conditioning and eye protection effects are enhanced. According to the additive, low-sodium and low-iodine carrier raw materials are further adopted, and a micronization dispersion treatment process is combined, so that the uniformity and palatability of the product in pet feed are improved. Through multiple synergistic effects of antioxidation, anti-inflammation, heat clearing and nutrition regulation, the pet feed can effectively relieve tear stains of pets from the level of nutrition regulation, and is suitable for long-term feeding of pets.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pet functional feed additives, and particularly relates to a selenium-rich yeast additive for reducing pet tear marks. BACKGROUND

[0002] In recent years, with the increase in the number of pet families, the awareness of pet health care is constantly improving, and pet appearance problems are particularly widely concerned. Among them, tear marks are one of the common appearance problems of cats and dogs, which are manifested as brown, wet and peculiar smell of hair around the eyes, not only affecting the appearance, but also reflecting the health risks of lacrimal gland abnormalities, eye inflammation or nutritional imbalance. The causes of tear marks are relatively complex, mainly including chronic irritation of the lacrimal gland, oxidative stress reaction around the eyes, repeated local inflammation, and excessive intake of sodium and iodine in feed leading to tear gland secretion disorder.

[0003] At present, the commercially available tear-reducing products are mainly eye drops, wipes, antibiotic additives or single antioxidant substances, which can temporarily relieve symptoms, but are difficult to fundamentally improve the systemic causes of tear marks in pets. In addition, some products have poor palatability, low compliance, and lack of scientific basis for ingredient proportioning, resulting in unstable long-term use effect and easy recurrence. Under this background, the development of a pet-specific composite additive with antioxidant, anti-inflammatory and heat-clearing, immune-regulating and eye tissue protection functions from the perspective of nutritional intervention has become a research hotspot in the field of pet nutrition. In particular, selenium-rich yeast as a high-quality source of organic selenium has good performance in removing free radicals and slowing down oxidative damage, and the synergistic combination of Chinese herbal extract and nutritional amino acids also provides a new solution for regulating lacrimal gland function. SUMMARY

[0004] To solve the problems mentioned in the background, the purpose of the present application is to provide a selenium-rich yeast additive for reducing pet tear marks, which is mainly composed of selenium-rich yeast, chrysanthemum extract, honeysuckle extract, cassia seed extract, L-lysine, taurine, vitamin C, lutein and low-sodium and low-iodine carriers, and has multiple functions such as antioxidant, anti-inflammatory and heat-clearing, and lacrimal gland conditioning, which can effectively relieve pet tear marks and is suitable for long-term feeding.

[0005] The purpose of the present application can be achieved by the following technical solutions: A selenium-rich yeast additive for reducing pet tear marks, comprising the following raw materials in parts by weight: 2-6 parts of selenium-rich yeast, 1-3 parts of chrysanthemum extract, 1-3 parts of honeysuckle extract, 1-2 parts of cassia seed extract, 1-3 parts of L-lysine, 1-2 parts of taurine, 0.5-2 parts of vitamin C, 0.5-1 part of lutein, and the balance of carrier raw materials composed of low-sodium corn starch and low-iodine wheat bran.

[0006] Further preferably, the organic selenium content in the selenium-rich yeast is not less than 1000 mg / kg.

[0007] Further preferably, the mass ratio of L-lysine to taurine is (2.5-3.5):(1.5-2.5), preferably 3:2.

[0008] Further preferably, the mass ratio of the selenium-rich yeast, the chrysanthemum extract, and the honeysuckle extract is (1.6-2.4):(0.8-1.2):(0.8-1.2), preferably 2:1:1.

[0009] Further preferably, the carrier raw material is composed of 30-50 parts of low-sodium corn starch and 10-20 parts of low-iodine wheat bran.

[0010] Further preferably, the sodium content in the carrier raw material is ≤0.3%, and the iodine content is ≤0.5 mg / kg.

[0011] A preparation method of a selenium-rich yeast additive for reducing pet tear marks, comprising the following steps: S1. Inoculating a high-yield selenium yeast strain into a selenium-containing culture medium for fermentation culture, and recovering the yeast cells after the end of the fermentation culture to obtain a selenium-rich yeast raw material; S2. Taking chrysanthemum extract, honeysuckle extract, and cassia extract, performing screening and crushing treatment to make them reach uniform fineness, and then mixing them according to a set ratio to obtain an anti-inflammatory and heat-clearing component mixture; S3. Taking L-lysine, taurine, vitamin C, and lutein, performing drying and sieving treatment on each component, and then placing them in a mixing device for premixing to obtain a nutrition-enhancing component mixture; S4. Adding the selenium-rich yeast, the anti-inflammatory and heat-clearing component mixture, and the nutrition-enhancing component mixture into the mixing device in sequence, and performing sufficient mixing to form a uniform functional composite material; S5. Adding low-sodium corn starch and low-iodine wheat bran into the composite material, mixing them uniformly, and then performing micronization treatment on the overall material to obtain the selenium-rich yeast additive.

[0012] Further preferably, the preparation of the selenium-rich yeast in step S1 specifically comprises: inoculating a high-yield selenium yeast strain into a selenium-containing culture medium, and performing fermentation at 28-30°C and pH 5.5-6.0 for 48-72 hours, and then performing solid-liquid separation and drying after the end of the fermentation to obtain the selenium-rich yeast.

[0013] Further preferably, the micronization treatment in step S5 adopts a low-temperature crushing process, specifically comprising: sending the uniformly mixed composite material into a cooling airflow crushing device, and performing crushing in a low-temperature environment, controlling the particle size of the finished product powder to be not greater than 200 mesh, and obtaining the selenium-rich yeast additive with uniform particle size.

[0014] Further preferably, the selenium-enriched yeast additive obtained after step S5 is in the form of a dry powder solid preparation, which is suitable for direct dry mixing with pet food.

[0015] Advantages of the present application: The selenium-enriched yeast additive for reducing tear marks of pets provided by the present application is characterized in that selenium-enriched yeast is used as a high bioavailability organic selenium source, which effectively improves the antioxidant capacity in the body of pets, removes free radicals in eye tissues, reduces the chronic stimulation of oxidative stress on the lacrimal gland, and achieves the purpose of relieving abnormal tear secretion from the source. Combined with three functional components of traditional Chinese medicine, i.e., chrysanthemum extract, honeysuckle extract and cassia seed extract, through the synergistic effect of clearing heat and reducing fire, anti-inflammatory and antibacterial, and improving eye microcirculation, the inflammation of lacrimal gland mucosa can be effectively reduced, and the composition of tear can be improved, so as to slow down the formation and deposition of brown tear marks around the eyes. On this basis, L-lysine and taurine are introduced in a scientific ratio of 3:2, which can inhibit the expression of inflammatory factors and assist in regulating immune response, further stabilizing the function of the lacrimal gland; the addition of vitamin C and lutein enhances the antioxidant barrier and retinal protection ability, forming a comprehensive care for eye tissues. The present application also adopts a low-sodium and low-iodine formula design to reduce the stimulation of the lacrimal gland from the aspect of mineral metabolism, effectively avoiding the problem of abnormal tear secretion induced by high salt or excessive iodine. At the same time, through the micronization process, a dry powder product with uniform distribution and appropriate particle size is prepared, which significantly improves its dispersibility and palatability in pet food, ensuring the consistency of nutrient intake and the stability of functional expression. BRIEF DESCRIPTION OF DRAWINGS

[0016] The present application will be further described below in conjunction with the drawings.

[0017] Figure 1 The comparison chart of antioxidant enzyme activity of animals after feeding examples 1-3 and comparative examples 1-2; Figure 2 The comparison chart of eye redness / tear secretion effect of animals after feeding examples 1-3 and comparative examples 1-2; Figure 3 The comparison chart of feed intake and feeding time of animals. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings of the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0019] Example 1 The selenium-rich yeast additive for reducing pet tear marks comprises the following raw materials in terms of weight parts: 2 parts of selenium-rich yeast, 1 part of chrysanthemum extract, 1 part of honeysuckle extract, 1 part of cassia seed extract, 1.8 parts of L-lysine, 1.2 parts of taurine, 0.5 parts of vitamin C, 0.5 parts of lutein, 30 parts of low-sodium corn starch, and 10 parts of low-iodine wheat bran.

[0020] The preparation steps of the selenium-rich yeast additive for reducing pet tear marks are as follows: S1. Select a high-selenium-tolerant yeast strain, inoculate in a selenium-containing culture medium, and set the fermentation conditions as follows: temperature 28℃, pH 5.5, aeration rate 1.0 vvm, stirring speed 120 rpm, and fermentation period 48 hours. After fermentation, centrifugal separation is performed, the yeast cells are recovered, dried at 40℃ to a water content of ≤5%, ground through an 80-mesh sieve, and reserved for use; S2. Weigh 10 g of chrysanthemum extract, 10 g of honeysuckle extract, and 10 g of cassia seed extract, dry them in a 50℃ hot air drying oven for 2 hours, cool them down, grind them through a 120-mesh sieve, and then mix them evenly to obtain an anti-inflammatory and heat-clearing component mixture; S3. Weigh 18 g of L-lysine, 12 g of taurine, 5 g of vitamin C, and 5 g of lutein, dry them in a 40℃ vacuum drying oven for 1 hour, cool them down, pass them through a 100-mesh sieve, and mix them in a high-speed mixer for 3 minutes to obtain a nutrient synergistic component mixture; S4. Add 20 g of selenium-rich yeast, 30 g of the anti-inflammatory and heat-clearing component mixture, and 40 g of the nutrient synergistic component mixture into a V-type mixer in sequence, set the mixing speed to 30 rpm, and mix for 15 minutes to obtain a functional composite material; S5. Weigh 300 g of low-sodium corn starch and 100 g of low-iodine wheat bran, pass them through a 100-mesh sieve, and then add them into the functional composite material, continue to mix for 10 minutes, and then send the mixture into a cooling jet mill, control the temperature to ≤35℃, and control the target particle size to below 200 mesh (about 75 μm) to obtain the selenium-rich yeast additive for reducing pet tear marks.

[0021] Example 2 The selenium-rich yeast additive for reducing pet tear marks comprises the following raw materials in terms of weight parts: 6 parts of selenium-rich yeast, 3 parts of chrysanthemum extract, 3 parts of honeysuckle extract, 2 parts of cassia seed extract, 3 parts of L-lysine, 2 parts of taurine, 2 parts of vitamin C, 1 part of lutein, 50 parts of low-sodium corn starch, and 20 parts of low-iodine wheat bran.

[0022] The preparation steps of the selenium-rich yeast additive for reducing pet tear marks are as follows: S1. Select high selenium tolerance yeast strain, inoculate it into selenium-containing medium for fermentation culture, fermentation conditions are set as follows: temperature 30℃, pH 6.0, stirring speed 150 rpm, aeration amount 1.2 vvm, fermentation time 72 hours. After fermentation, centrifugal separation is carried out on the culture solution, and the yeast cells are recovered; then the yeast cells are placed in a 45℃ hot air drying oven for drying until the moisture content is ≤5%, crushed and passed through an 80 mesh sieve for standby; S2. Take 30 g of chrysanthemum extract, 30 g of honeysuckle extract and 20 g of cassia seed extract, dry them at 55℃ for 2 hours, cool to room temperature, and then crush and pass through a 120 mesh sieve respectively; then add the three into a mixing container in proportion and mix uniformly to obtain an anti-inflammatory and heat-clearing component mixture; S3. Take 30 g of L-lysine, 20 g of taurine, 20 g of vitamin C and 10 g of lutein, dry each component in a 40℃ vacuum drying oven for 1 hour, cool and then pass through a 100 mesh sieve respectively; then add each component into a high-speed mixer and mix at a speed of 1500 rpm for 5 minutes to obtain a uniform nutrient and synergistic component mixture; S4. Add 60 g of selenium-rich yeast, 80 g of anti-inflammatory and heat-clearing component mixture and 80 g of nutrient and synergistic component mixture into a V-type mixer in sequence, set the mixing speed to 35 rpm and the mixing time to 20 minutes to obtain a uniform functional composite material; S5. Take 500 g of low-sodium corn starch and 200 g of low-iodine wheat bran, pass them through a 100 mesh sieve and then add them into the functional composite material, mix for 15 minutes, and then send the mixture for micronization treatment, control the crushing environment temperature to ≤35℃, and control the particle size of the finished powder to be below 200 mesh to obtain the selenium-rich yeast additive for reducing pet tear marks.

[0023] Example 3 The selenium-rich yeast additive for reducing pet tear marks comprises the following raw materials in parts by weight: 4 parts of selenium-rich yeast, 2 parts of chrysanthemum extract, 2 parts of honeysuckle extract, 1.5 parts of cassia seed extract, 2.4 parts of L-lysine, 1.6 parts of taurine, 1.25 parts of vitamin C, 0.75 parts of lutein, 40 parts of low-sodium corn starch and 15 parts of low-iodine wheat bran.

[0024] The preparation steps of the selenium-rich yeast additive for reducing pet tear marks are as follows: S1. Ferment a high organic selenium content yeast strain, the fermentation conditions are as follows: temperature 29℃, pH 5.8, aeration amount 1.1 vvm, stirring 120 rpm, and fermentation time 60 hours. After fermentation, centrifugal separation is carried out to obtain yeast cells, which are dried at 45℃ to a moisture content of ≤5%, crushed and passed through an 80 mesh sieve, and then 40 g of the yeast cells are taken for standby; S2. Take 20 g of chrysanthemum extract, 20 g of honeysuckle extract, and 15 g of cassia seed extract, and place them in a 50℃ hot air drying oven for 2 hours. After cooling, crush to 120 mesh. Mix according to the proportion to obtain an anti-inflammatory and heat-clearing component mixture; S3. Take 24 g of L-lysine, 16 g of taurine, 12.5 g of vitamin C, and 7.5 g of lutein, and place them in a 40℃ vacuum drying oven for 1 hour. After cooling, pass through a 100 mesh sieve. Then mix the components at 1500 rpm for 5 minutes to obtain a nutrient synergistic component mixture; S4. Add 40 g of selenium-rich yeast, 55 g of anti-inflammatory and heat-clearing component mixture, and 60 g of nutrient synergistic component mixture to a V-type mixer in sequence. Set the mixing speed to 30 rpm and the mixing time to 20 minutes to obtain a uniform functional composite material; S5. Take 400 g of low-sodium corn starch and 150 g of low-iodine wheat bran, pass them through a 100 mesh sieve, and then add them to the functional composite material and mix for 10 minutes. Then send the mixture for micronization treatment, control the crushing environment temperature ≤ 35℃, and control the particle size of the finished powder to be below 200 mesh to obtain the selenium-rich yeast additive for reducing pet tear marks.

[0025] Comparative Example 1 The additive for reducing pet tear marks comprises the following raw materials by weight: chrysanthemum extract 2 parts, honeysuckle extract 2 parts, cassia seed extract 1.5 parts, L-lysine 2.4 parts, taurine 1.6 parts, vitamin C 1.25 parts, lutein 0.75 parts, low-sodium corn starch 40 parts, and low-iodine wheat bran 15 parts.

[0026] The preparation steps of the additive for reducing pet tear marks are the same as those of Example 3 except that there is no step S1 of preparing and adding selenium-rich yeast.

[0027] Comparative Example 2 The selenium-rich yeast additive for reducing pet tear marks comprises the following raw materials by weight: selenium-rich yeast 4 parts, chrysanthemum extract 2 parts, honeysuckle extract 2 parts, cassia seed extract 1.5 parts, L-lysine 2.4 parts, taurine 1.6 parts, vitamin C 1.25 parts, lutein 0.75 parts, ordinary starch 40 parts, and ordinary wheat bran 15 parts.

[0028] The preparation steps of the additive for reducing pet tear marks are the same as those of Example 3 except that in step S5, low-sodium corn starch is replaced by ordinary starch and low-iodine wheat bran is replaced by ordinary wheat bran.

[0029] Performance Test 1. Test of tear mark reduction effect Dogs and cats with obvious tear stains and good health were selected as experimental subjects. Before the experiment, they were uniformly fed a basal diet for 7 days as an acclimatization period, and the initial tear stain condition of each animal was recorded. During the experiment, the additives prepared in Examples 1-3 and Comparative Examples 1-2 were added to the basal diet, fed daily in fixed quantities for 14 consecutive days. Before and after the experiment, the tear stains around the eyes of the animals were assessed, mainly observing changes in tear stain color depth, length, and coverage area. A 0-5 point tear stain scoring method was used for quantitative evaluation, where 0 points indicated no obvious tear stains and 5 points indicated severe and obvious tear stains. The results are shown in Table 1 below.

[0030] Table 1. Tear stain reduction effect As shown in Table 1, after 14 days of continuous feeding, the tear stain scores of Examples 1-3 were significantly lower than the initial scores, indicating that the additive of the present invention can effectively reduce tear stains around the eyes of pets. Among them, the tear stain score of Example 3 decreased from 4.2 to 2.0, a reduction of 2.2, with a tear stain improvement rate of 52.4%, showing the best improvement effect. Although the dosage of Example 1 was lower, the tear stain improvement rate still reached 33.3%, indicating that the present invention still has a certain effect at a low dosage. The tear stain scores of Comparative Examples 1 and 2 decreased significantly. The improvement rate of Comparative Example 1 without selenium-enriched yeast was only 16.3%, indicating that selenium-enriched yeast played a key role in alleviating tear stains.

[0031] 2. Antioxidant capacity test Dogs and cats with obvious tear stains and good health were selected as experimental subjects. After being fed a uniform basal diet for 7 days, they were divided into the example group and the control group, with the corresponding additive products added to each group. They were fed a fixed amount of food daily for 14 consecutive days. After the experiment, venous blood was collected from the animals, and serum was separated. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) in the serum were detected using an ELISA kit. The results are shown in Table 2 below.

[0032] Table 2 Antioxidant Capacity Results As can be seen from Table 2, the effects of different additive formulations on the antioxidant levels in animals are obviously different. After 14 days of continuous feeding, the SOD and GSH-Px activities in the serum of Examples 1-3 are significantly higher than those of the comparative examples, and the MDA content is significantly reduced, indicating that the additive of the application can effectively enhance the antioxidant defense ability in animals. Among them, the SOD activity and GSH-Px activity of Example 3 are 152 U / mL and 218 U / mL respectively, and the MDA content is the lowest, only 2.8 nmol / mL, showing the best antioxidant effect; while the antioxidant enzyme activity of Comparative Example 1 is significantly reduced and the MDA content is the highest due to the absence of selenium-rich yeast, indicating that selenium-rich yeast plays a key role in improving the antioxidant capacity of the body; the antioxidant effect of Comparative Example 2 is also significantly weaker than that of the example group, further indicating that the synergistic design of low-sodium and low-iodine carriers and functional components helps to maintain the redox balance in the body.

[0033] 3. Eye redness / tear secretion Select dogs and cats with obvious tear marks and good health as experimental objects, and after 7 days of adaptive feeding of the basic daily ration, divide them into the example group and the comparative example group. Respectively, the selenium-rich yeast additive is fed quantitatively every day for 14 days. On the 0th day and the 14th day, record the degree of conjunctival redness and abnormality of tear secretion of each animal, and use 0-3 points to make a comprehensive score: 0 points for normal, 1 point for slight redness or slight tear abnormality, 2 points for moderate, and 3 points for severe redness or secretion abnormality. The results are shown in Table 3 below.

[0034] Table 3 Eye redness and tear abnormality score results As can be seen from Table 3, after 14 days of continuous feeding, the eye comprehensive score of Examples 1-3 is significantly lower than the initial score, indicating that the selenium-rich yeast additive of the application can effectively reduce the conjunctival redness and improve the tear secretion abnormality. Among them, the score of Example 3 is reduced from 2.8 to 1.0, with a score reduction value of 1.8, showing the best improvement effect; while the score of Comparative Example 1 after the test is still 2.3, only reduced by 0.5, indicating that selenium-rich yeast plays a key role in antioxidant and mucosa conditioning, and its absence significantly weakens the overall efficacy of the product. The score reduction value of Comparative Example 2 is also lower than that of the example group, indicating that the low-sodium and low-iodine carrier can effectively reduce the stimulation of lacrimal gland and mineral interference, and is an important part of improving the overall synergistic effect of the formulation.

[0035] 4. Palatability test Dogs and cats with obvious tear marks and normal feeding behavior were selected as experimental subjects, and after 7 days of adaptive feeding of the basic diet, they were divided into Examples 1-3 and Comparative Examples 1-2. Each group was added with the corresponding Example and Comparative Example selenium-rich yeast additive in a fixed proportion in the basic diet, and a free-feeding test was conducted. The same amount of feed was fed at a fixed time every day, and the actual feed intake and the time required to complete the feeding of the animals were recorded, and this was done continuously for 7 days. The palatability score was analyzed using the average daily feed intake (g) and average feeding time (min) as indicators, and the results are shown in Table 4.

[0036] Table 4 Palatability test results As can be seen from Table 4, the average daily feed intake of the animals in Examples 1-3 was higher than that of the comparative examples, and the feeding time was significantly shortened, indicating that the selenium-rich yeast additive prepared by the present application has good palatability under different formula conditions. Among them, the average feed intake of Example 3 was 175 g, and the feeding time was only 4.4 minutes, which performed best, indicating that under the synergistic effect of selenium-rich yeast, vitamin C, lutein and other functional ingredients, not only did it not affect the flavor of the feed, but it also improved the feeding enthusiasm of the animals. After removing the selenium-rich yeast in Comparative Example 1, the animal feed intake decreased to 160 g, and the feeding time increased to 6.3 minutes, indicating that the organic selenium in the selenium-rich yeast may play a positive role in regulating taste sensitivity and enhancing feeding behavior; while the use of ordinary carriers in Comparative Example 2 resulted in a more significant decrease in feed intake, indicating that the carrier system composed of low-sodium corn starch and low-iodine bran has important significance in controlling mineral stimulation and maintaining the palatability of the feed.

[0037] In the description of the present specification, the description of the terms "one embodiment", "example", "specific example" and the like means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0038] The basic principles, main features and advantages of the present application are shown and described above. Those skilled in the art should understand that the present application is not limited by the above examples, and the above examples and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application.

Claims

1. A selenium-enriched yeast additive for reducing tear stains in pets, characterized in that, It contains the following ingredients by weight: 2-6 parts selenium-enriched yeast, 1-3 parts chrysanthemum extract, 1-3 parts honeysuckle extract, 1-2 parts cassia seed extract, 1-3 parts L-lysine, 1-2 parts taurine, 0.5-2 parts vitamin C, 0.5-1 part lutein, and the remainder is a carrier ingredient composed of low-sodium corn starch and low-iodine wheat bran.

2. The selenium-enriched yeast additive according to claim 1, characterized in that, The selenium-enriched yeast contains no less than 1000 mg / kg of organic selenium.

3. The selenium-enriched yeast additive according to claim 1, characterized in that, The mass ratio of L-lysine to taurine is (2.5-3.5):(1.5-2.5), preferably 3:

2.

4. The selenium-enriched yeast additive according to claim 1, characterized in that, The mass ratio of the selenium-enriched yeast, chrysanthemum extract, and honeysuckle extract is (1.6-2.4):(0.8-1.2):(0.8-1.2), preferably 2:1:

1.

5. The selenium-enriched yeast additive according to claim 1, characterized in that, The carrier material consists of 30-50 parts of low-sodium corn starch and 10-20 parts of low-iodine wheat bran.

6. The selenium-enriched yeast additive according to claim 1, characterized in that, The carrier raw material has a sodium content of ≤0.3% and an iodine content of ≤0.5mg / kg.

7. A method for preparing a selenium-enriched yeast additive for reducing tear stains in pets, the selenium-enriched yeast additive as described in any one of claims 1 to 6, characterized in that, Includes the following steps: S1. A high-selenium-producing yeast strain was inoculated into a selenium-containing medium for fermentation culture. After the fermentation culture was completed, the yeast cells were recovered to obtain selenium-enriched yeast raw material. S2. Take chrysanthemum extract, honeysuckle extract and cassia seed extract, sieve and pulverize them to achieve uniform fineness, then mix them in a set ratio to obtain an anti-inflammatory and heat-clearing component mixture. S3. Take L-lysine, taurine, vitamin C and lutein, dry and sieve each component, and then place them in a mixing device for premixing to obtain a mixture of nutrient-enhancing components; S4. Sequentially add the selenium-enriched yeast, the anti-inflammatory and heat-clearing component mixture, and the nutritional enhancement component mixture into the mixing equipment and mix them thoroughly to form a uniform functional compound material; S5. Add low-sodium corn starch and low-iodine wheat bran to the compound material, mix evenly, and then perform micronization treatment on the whole material, namely the selenium-enriched yeast additive.

8. The method for preparing the selenium-enriched yeast additive according to claim 7, characterized in that, The preparation of selenium-enriched yeast in step S1 specifically includes: inoculating a high-selenium-producing yeast strain into a selenium-containing culture medium, fermenting it at 28-30℃ and pH 5.5-6.0 for 48-72 hours, and after fermentation, performing solid-liquid separation and drying to obtain selenium-enriched yeast.

9. The method for preparing the selenium-enriched yeast additive according to claim 7, characterized in that, The micronization process described in step S5 employs a low-temperature pulverization process, specifically including: feeding the uniformly mixed composite material into a cooled airflow pulverizer, pulverizing it in a low-temperature environment, controlling the particle size of the finished powder to be no greater than 200 mesh, and obtaining a selenium-enriched yeast additive with uniform particle size.

10. The method for preparing the selenium-enriched yeast additive according to claim 7, characterized in that, The selenium-enriched yeast additive obtained after step S5 is a dry powder solid preparation, suitable for direct dry mixing with pet food.

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