Whitening and sunscreen composition containing synotis erythropappa and preparation method of whitening and sunscreen composition
By combining natural extracts such as red chrysanthemum and Tibetan calamus with microbial fermentation, a whitening and sun protection composition was prepared, which solved the problems of single function and high irritation of existing products, and achieved a synergistic effect of highly effective whitening and sun protection.
Patent Information
- Application Number
- CN202511792161.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-01
- Publication Date
- 2026-02-27
AI Technical Summary
Existing whitening and sun protection products have limited functions, most of which use chemical ingredients that are highly irritating to the skin and cannot effectively alleviate skin damage and aging caused by ultraviolet rays.
A whitening and sun protection composition is prepared by combining natural extracts such as red chrysanthemum, Tibetan calamus, and lygodium japonicum with specific microbial fermentation. Through synergistic effects, it enhances the inhibition of tyrosinase and the absorption of ultraviolet rays, thereby reducing melanin production and moisture loss.
It effectively enhances whitening effects, strengthens sun protection, reduces UV irritation, repairs skin damage, and enhances the skin's resistance to external stimuli.
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Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of whitening sunscreen products, in particular to a whitening sunscreen composition containing red and yellow ear chrysanthemum and a preparation method thereof. BACKGROUND
[0002] Long-term ultraviolet radiation and other factors can cause the skin to be easily sunburned, tanned and aged. With the improvement of living standards, people pay more and more attention to their skin problems, and white and tender skin has always been the pursuit of people. Therefore, sunscreen products and whitening products are more common in daily necessities. However, at present, most of the products have relatively single functions, and often only have the functions of sun protection or whitening, and most of the whitening or sunscreen ingredients are chemical ingredients, which can cause strong irritation to the skin. And some sunscreen products are greasy and closed during use, which can easily cause burden to the skin, and the whitening effect is also very limited, and it is also unable to alleviate the skin damage, aging and other problems caused by ultraviolet radiation. SUMMARY
[0003] In view of this, the present application provides a whitening sunscreen composition containing red and yellow ear chrysanthemum and a preparation method thereof.
[0004] The technical scheme of the present application is as follows: A whitening sunscreen composition containing red and yellow ear chrysanthemum, comprising the following raw materials by weight: red and yellow ear chrysanthemum extract 17-20 parts, aconite extract 10-13 parts, Gentiana straminea Maxim extract 12-16 parts, high mountain fireweed extract 13-15 parts, sea gold sand extract 11-14 parts, sea buckthorn extract 13-15 parts, nicotinamide 5-8 parts, collagen 4-7 parts, sodium hyaluronate 1.5-3 parts, squalane 4-9 parts, decyl glucoside 1-3 parts, lactobacillus fermentation product 6-9 parts, ethylene glycol distearate 3-5 parts, sorbitan olivate 2-3 parts, Tween 80 4-6 parts, 1,3-propanediol 9-12 parts, and water 70-80 parts.
[0005] Preferably, the red and yellow ear chrysanthemum extract is obtained by fermenting red and yellow ear chrysanthemum with Eurotium cristatum.
[0006] Preferably, the preparation method of the red and yellow ear chrysanthemum extract is as follows: the red and yellow ear chrysanthemum is crushed into powder, then activated Eurotium cristatum bacterial suspension and culture medium are added and stirred uniformly, fermentation culture is carried out, sterilization is carried out, and then a crude fermentation liquor is obtained, the crude fermentation liquor is centrifuged, the precipitate and bacterial bodies are discarded, and the liquid is concentrated to obtain the red and yellow ear chrysanthemum extract.
[0007] Preferably, the mass ratio of the powder, the Eurotium cristatum bacterial suspension and the culture medium is 23-27:0.1-0.2:100; the concentration of the Eurotium cristatum bacterial suspension is 10 5~10 6 CFU / mL.
[0008] Preferably, the fermentation culture temperature is 28~30℃, the rotation speed is 120~150r / min, and the time is 95~100h; the centrifugal rotation speed is 1200~1500r / min, and the time is 10~12min.
[0009] Preferably, the preparation method of the Acorus calamus extract is as follows: the Acorus calamus is crushed into Acorus calamus powder, which is mixed with water to obtain a mixture, the fermentation bacteria suspension is added to the mixture and mixed, and then fermentation is carried out, sterilization is performed, and a fermentation liquid is obtained. The fermentation bacteria are centrifuged, and the precipitate and bacteria are discarded. The liquid is concentrated to obtain the Acorus calamus extract.
[0010] Preferably, the ratio of the Acorus calamus powder to water is 1:9~12 g / mL; the amount of the fermentation bacteria suspension added is 4~6% v / v of the mixture; and the fermentation temperature is 30~32℃, the rotation speed is 140~160r / min, and the time is 48~72h.
[0011] Preferably, the bacteria in the fermentation bacteria suspension include 4~6:8~10 by mass ratio of Sporidiobolus pararoseus and Lactobacillus rhamnosus; the concentration of the Sporidiobolus pararoseus is 10 6 ~10 7 CFU / mL; and the concentration of the Lactobacillus rhamnosus is 10 5 ~10 7 CFU / mL.
[0012] The preparation method of the whitening sunscreen composition containing the Saxifraga oppositifolia includes the following steps: (1) Squalane, ethylene glycol distearate, and sorbitan olivate are stirred and mixed to obtain a mixture; (2) Tween 80, 1,3-propanediol, and water are stirred and mixed, and then Saxifraga oppositifolia extract, Acorus calamus extract, Gentiana macrophylla extract, Edelweiss extract, Sargassum fusiforme extract, Hippophae rhamnoides extract, nicotinamide, collagen, and sodium hyaluronate are added and stirred again. Finally, the mixture in step (1) is added and stirred to obtain the target whitening sunscreen composition.
[0013] Preferably, in step (1), the stirring rotation speed is 150~180r / min, the temperature is 60~70℃, and the time is 8~10min; and in step (2), the stirring rotation speed is 200~320r / min, the temperature is 60~65℃, and the time is 15~20min.
[0014] Preferably, the culture medium is CZG liquid medium; the CZG liquid medium formula is: sucrose 40g / L, dipotassium phosphate 1g / L, magnesium sulfate 0.5g / L, ammonium nitrate 3g / L, sodium chloride 50g / L, distilled water 1000mL; the pH is natural.
[0015] Compared with the prior art, the present application has the following advantages: The composition of the present application uses red and yellow ear chrysanthemum extract, aconite extract, sea gold sand extract and the like as raw materials and mixes them in a specific proportion, so that the active ingredients have a synergistic effect, can effectively improve the inhibition of tyrosinase activity, reduce the oxidative stress damage of melanocytes, prevent the generation of melanin, reduce the growth of black spots on the skin after sun exposure, reduce pigmentation, improve the whitening effect, and can improve the ultraviolet absorption capacity of the composition, reduce the penetration of ultraviolet rays into the skin, improve the sun protection effect, and also can reduce the irritation of ultraviolet rays, reduce skin moisture loss, repair skin damage, soothe the skin, and improve the resistance to external stimuli.
[0016] In addition, the present application uses Eurotium cristatum for fermentation when preparing red and yellow ear chrysanthemum extract, and uses Sporidiobolus rupestris and Lactobacillus rhamnosus for fermentation of aconite extract, which can effectively enrich the active ingredients in red and yellow ear chrysanthemum and aconite, and better improve the activity of the active ingredients in red and yellow ear chrysanthemum and aconite, effectively promoting the effect of the composition in whitening and sun protection. DETAILED DESCRIPTION
[0017] In order to better understand the technical content of the present application, the following specific examples are provided to further illustrate the present application.
[0018] The experimental methods used in the embodiments of the present application are conventional methods unless otherwise specified.
[0019] The materials, reagents and the like used in the embodiments of the present application can be obtained from commercial channels unless otherwise specified.
[0020] The raw materials of the plant extract of the present application are as follows: Aconite extract (ACORUS CALAMUS), Gentiana macrophylla extract, Leontopodium alpinum extract, Hippophae rhamnoides extract, LYGODIUM JAPONICUM extract.
[0021] Eurotium cristatum is purchased from Wuhan Zhanyan Huabiao Technology Co., Ltd., and the preservation number is CICC 2099.
[0022] Yarrowia sp. was purchased from Shanghai Center for Applied
[0023] Lactobacillus rhamnosus was purchased from Shaanxi Boundary
[0024] Example 1 The whitening sunscreen composition containing the Ligularia sibirica in this example comprises the following raw materials by weight: Ligularia sibirica extract 20 parts, Acorus gramineus extract 12 parts, Gentiana macrophylla extract 15 parts, Leontopodium alpinum extract 13 parts, Salsola komarovii extract 12 parts, Hippophae rhamnoides extract 15 parts, nicotinamide 8 parts, collagen 4 parts, sodium hyaluronate 1.5 parts, squalane 9 parts, decyl glucoside 2 parts, Lactobacillus ferment 6 parts, ethylene glycol distearate 4 parts, sorbitan olivate 2 parts, Tween 80 6 parts, 1,3-propanediol 10 parts, purified water 70 parts.
[0025] The preparation method of the Ligularia sibirica extract is as follows: the Ligularia sibirica is crushed into powder, then activated Eurotium cristatum bacterial suspension and CZG liquid medium are added and stirred uniformly (wherein the mass ratio of the powder, the Eurotium cristatum bacterial suspension and the medium is 23:0.2:100, the concentration of the Eurotium cristatum bacterial suspension is 10 6 CFU / mL, and the formula of the CZG liquid medium is: sucrose 40 g / L, di-potassium phosphate 1 g / L, magnesium sulfate 0.5 g / L, ammonium nitrate 3 g / L, sodium chloride 50 g / L, distilled water 1000 mL; the pH is natural), and the fermentation culture is carried out at a temperature of 28-30°C and a rotation speed of 120 r / min for 95 h, sterilized, to obtain a crude fermentation liquor, which is then centrifuged at a rotation speed of 1400 r / min for 12 min, and the precipitate and bacterial body are discarded, and the liquid is concentrated, to obtain the Ligularia sibirica extract.
[0026] The preparation method of the Acorus gramineus extract is as follows: the Acorus gramineus is crushed into Acorus gramineus powder, which is then mixed with purified water to obtain a mixture (wherein the solid-liquid ratio of the Acorus gramineus powder and the purified water is 1:12 g / mL), and the fermentation bacterial suspension is inoculated into the mixture and stirred uniformly, and then the fermentation is carried out at a temperature of 30°C and a rotation speed of 150 r / min for 48 h (wherein the inoculation amount of the fermentation bacterial suspension is 5% v / v of the mixture, and the bacterial species in the fermentation bacterial suspension include Yarrowia sp. and Lactobacillus rhamnosus in a mass ratio of 6:10; the concentration of the Yarrowia sp. is 10 6 CFU / mL; and the concentration of the Lactobacillus rhamnosus is 10 7 CFU / mL), sterilized, to obtain a fermentation liquor, which is then centrifuged at a rotation speed of 1500 r / min for 10 min, and the precipitate and bacterial body are discarded, and the liquid is concentrated, to obtain the Acorus gramineus extract.
[0027] The preparation method of the whitening sunscreen composition containing the red-tasseled ear mushroom includes the following steps: (1) The squalane, ethylene glycol distearate, sorbitan olivate are stirred and mixed uniformly at a speed of 180 r / min and a temperature of 60°C for 8 min to obtain a mixture; (2) The Tween 80, 1,3-propanediol and purified water are stirred and mixed uniformly at a speed of 300 r / min and a temperature of 65°C for 20 min, then the red-tasseled ear mushroom extract, Acorus gramineus Soland extract, Gentiana straminea Maxim extract, Edelweiss extract, Salsola Kallikangr extract, Hippophae rhamnoides Linn extract, nicotinamide, collagen, sodium hyaluronate are added and stirred and mixed uniformly for 15 min, and finally the mixture in step (1) is added and stirred and mixed uniformly for 15 min to obtain the composition.
[0028] Example 2 The whitening sunscreen composition containing the red-tasseled ear mushroom of the present example includes the following raw materials by weight: red-tasseled ear mushroom extract 17 parts, Acorus gramineus Soland extract 13 parts, Gentiana straminea Maxim extract 16 parts, Edelweiss extract 14 parts, Salsola Kallikangr extract 11 parts, Hippophae rhamnoides Linn extract 13 parts, nicotinamide 5 parts, collagen 5 parts, sodium hyaluronate 3 parts, squalane 8 parts, decyl glucoside 1 part, lactobacillus fermentation product 7 parts, ethylene glycol distearate 5 parts, sorbitan olivate 3 parts, Tween 80 4 parts, 1,3-propanediol 12 parts, and purified water 80 parts.
[0029] The preparation method of the red-tasseled ear mushroom extract is as follows: the red-tasseled ear mushroom is crushed into powder, then activated Eurotium cristatum bacterial suspension and CZG liquid medium are added and stirred uniformly (wherein the mass ratio of the powder, Eurotium cristatum bacterial suspension and medium is 27:0.1:100, and the concentration of the Eurotium cristatum bacterial suspension is 10 5 CFU / mL), and the fermentation culture is carried out at a temperature of 29°C and a speed of 150 r / min for 100 h, then sterilized to obtain a crude fermentation liquid, which is then centrifuged at a speed of 1500 r / min for 10 min, then the precipitate and bacterial body are discarded, and the liquid is concentrated to obtain the red-tasseled ear mushroom extract.
[0030] The preparation method of the Acorus gramineus Soland extract is as follows: the Acorus gramineus Soland is crushed into Acorus gramineus Soland powder, which is then mixed with purified water to obtain a mixture (wherein the solid-liquid ratio of the Acorus gramineus Soland powder and purified water is 1:9 g / mL), then the fermentation bacterial suspension is added to the mixture and stirred uniformly, and the fermentation is carried out at a temperature of 32°C and a speed of 160 r / min for 48 h (wherein the amount of the fermentation bacterial suspension added is 4% v / v of the mixture, and the bacterial species in the fermentation bacterial suspension include Saccharomycopsis fibuligera and Lactobacillus rhamnosus in a mass ratio of 5:8; the concentration of the Saccharomycopsis fibuligera is 10 7 CFU / mL; and the concentration of the Lactobacillus rhamnosus is 105 CFU / mL), sterilized, to obtain a fermentation broth, centrifuging the fermentation bacteria at a speed of 1300 r / min for 12 min, then discarding the precipitate and the bacterial bodies, concentrating the liquid to obtain the extract of Acorus calamus.
[0031] The preparation method of the whitening sunscreen composition containing the red-tasseled hybrid ear chrysanthemum includes the following steps: (1) Stir and mix squalane, ethylene glycol distearate, and sorbitan olivate at a stirring speed of 150 r / min and a temperature of 70℃ for 10 min to obtain a mixture; (2) Stir and mix Tween 80, 1,3-propanediol, and purified water at a stirring speed of 200 r / min and a temperature of 65℃ for 18 min, then add the extract of red-tasseled hybrid ear chrysanthemum, the extract of Acorus calamus, the extract of Gentiana macrophylla, the extract of high mountain fire Chirita, the extract of sea silver sand, the extract of Hippophae rhamnoides, nicotinamide, collagen, and sodium hyaluronate, and stir and mix again for 20 min, finally add the mixture in step (1) and stir and mix for 20 min to obtain the composition.
[0032] Example 3 The whitening sunscreen composition containing the red-tasseled hybrid ear chrysanthemum of the present example includes the following raw materials by weight: 18 parts of the extract of red-tasseled hybrid ear chrysanthemum, 10 parts of the extract of Acorus calamus, 12 parts of the extract of Gentiana macrophylla, 15 parts of the extract of high mountain fire Chirita, 14 parts of the extract of sea silver sand, 14 parts of the extract of Hippophae rhamnoides, 6 parts of nicotinamide, 7 parts of collagen, 2 parts of sodium hyaluronate, 4 parts of squalane, 3 parts of decyl glucoside, 9 parts of the fermentation product of lactobacillus, 3 parts of ethylene glycol distearate, 2 parts of sorbitan olivate, 5 parts of Tween 80, 9 parts of 1,3-propanediol, and 75 parts of purified water.
[0033] The preparation method of the extract of red-tasseled hybrid ear chrysanthemum is as follows: the red-tasseled hybrid ear chrysanthemum is crushed into powder, then activated Eurotium cristatum bacterial suspension and CZG liquid medium are added and stirred uniformly (wherein the mass ratio of the powder, the Eurotium cristatum bacterial suspension, and the medium is 25:0.15:100, and the concentration of the Eurotium cristatum bacterial suspension is 10 6 CFU / mL), fermentation culture is carried out at a temperature of 28℃ and a stirring speed of 140 r / min for 98 h, sterilization is performed to obtain a crude fermentation broth, then centrifugation is carried out at a speed of 1200 r / min for 12 min, the precipitate and the bacterial bodies are discarded, the liquid is concentrated to obtain the extract of red-tasseled hybrid ear chrysanthemum.
[0034] The preparation method of the B. tatarinowii extract includes the following steps: crushing B. tatarinowii into B. tatarinowii powder, mixing the B. tatarinowii powder with purified water to obtain a mixture (wherein the ratio of B. tatarinowii powder to purified water is 1:10 g / mL), mixing the mixture with a fermentation bacteria suspension, and then performing fermentation at a temperature of 30°C and a rotation speed of 140 r / min for 48 h (wherein the amount of the fermentation bacteria suspension is 6% v / v of the mixture, the fermentation bacteria suspension contains 4:9 of Mortierella isabellina and Lactobacillus rhamnosus by mass ratio, the concentration of the M. isabellina is 10 6 CFU / mL, and the concentration of the L. rhamnosus is 10 6 CFU / mL), sterilizing, centrifuging the fermentation bacteria at a rotation speed of 1400 r / min for 10 min, discarding the precipitate and the bacteria, and concentrating the liquid to obtain the B. tatarinowii extract.
[0035] The preparation method of the whitening sunscreen composition containing the S. rubroviolacea includes the following steps: (1) mixing squalane, ethylene glycol distearate, and sorbitan olivate at a rotation speed of 170 r / min and a temperature of 65°C for 9 min to obtain a mixture; (2) mixing Tween 80, 1,3-propanediol, and purified water at a rotation speed of 250 r / min and a temperature of 60°C for 15 min, then adding S. rubroviolacea extract, B. tatarinowii extract, G. maximowiczii extract, E. lacerum extract, C. officinalis extract, Hippophae rhamnoides extract, nicotinamide, collagen, and sodium hyaluronate, stirring and mixing for 18 min again, finally adding the mixture in step (1) and stirring and mixing for 15 min to obtain the composition.
[0036] Comparative Example 1 The difference between the present comparative example and Example 1 is that the raw material ratio of the composition is not the same, and the rest is the same.
[0037] The whitening sunscreen composition containing the S. rubroviolacea of the present comparative example includes the following raw materials by weight: S. rubroviolacea extract 10 parts, B. tatarinowii extract 6 parts, G. maximowiczii extract 9 parts, E. lacerum extract 22 parts, C. officinalis extract 5 parts, Hippophae rhamnoides extract 15 parts, nicotinamide 8 parts, collagen 4 parts, sodium hyaluronate 8.5 parts, squalane 9 parts, decyl glucoside 6 parts, Lactobacillus ferment 6 parts, ethylene glycol distearate 13 parts, sorbitan olivate 2 parts, Tween 80 6 parts, 1,3-propanediol 10 parts, and purified water 70 parts.
[0038] Comparative Example 2 The difference between the present comparative example and Example 1 is that the composition does not contain S. rubroviolacea extract in the raw material, and the rest is the same.
[0039] The whitening sunscreen composition containing the red-flowered Rabdosia japonica of the present comparative example comprises the following raw materials by weight: Acorus gramineus extract 17 parts, Gentiana macrophylla extract 20 parts, Edelweiss extract 18 parts, Sargassum fusiforme extract 17 parts, Hippophae rhamnoides extract 15 parts, Nicotinamide 8 parts, Collagen 4 parts, Sodium hyaluronate 1.5 parts, Squalane 9 parts, Decyl glucoside 2 parts, Lactobacillus ferment 6 parts, Ethylene glycol distearate 4 parts, Sorbitan olivate 2 parts, Tween 80 6 parts, 1,3-propanediol 10 parts, purified water 70 parts.
[0040] Comparative Example 3 The present comparative example differs from Example 1 in that the preparation method of the red-flowered Rabdosia japonica extract is not the same, and the rest remains the same.
[0041] The preparation method of the red-flowered Rabdosia japonica extract is as follows: The red-flowered Rabdosia japonica is crushed into powder, then mixed with an ethanol solution with a concentration of 75% v / v at a solid-liquid ratio of 1:10 g / mL, and then reflux extracted for 3 h, repeating the operation twice, combining all the filtrates, and then distilling under reduced pressure to obtain the red-flowered Rabdosia japonica extract.
[0042] Comparative Example 4 The present comparative example differs from Example 1 in that the composition does not contain Acorus gramineus extract in the raw materials, and the rest remains the same.
[0043] The whitening sunscreen composition containing the red-flowered Rabdosia japonica of the present comparative example comprises the following raw materials by weight: Acorus gramineus extract 17 parts, Gentiana macrophylla extract 20 parts, Edelweiss extract 18 parts, Sargassum fusiforme extract 17 parts, Hippophae rhamnoides extract 15 parts, Nicotinamide 8 parts, Collagen 4 parts, Sodium hyaluronate 1.5 parts, Squalane 9 parts, Decyl glucoside 2 parts, Lactobacillus ferment 6 parts, Ethylene glycol distearate 4 parts, Sorbitan olivate 2 parts, Tween 80 6 parts, 1,3-propanediol 10 parts, purified water 70 parts.
[0044] Comparative Example 5 The present comparative example differs from Example 1 in that the preparation method of the Acorus gramineus extract is not the same, and the rest remains the same.
[0045] The preparation method of the Acorus gramineus extract is as follows: The Acorus gramineus is crushed into Acorus gramineus powder, then mixed with an ethanol solution with a concentration of 75% v / v at a solid-liquid ratio of 1:10 g / mL, and then reflux extracted for 3 h, repeating the operation twice, combining all the filtrates, and then distilling under reduced pressure to obtain the Acorus gramineus extract.
[0046] Comparative Example 6 The present comparative example differs from Example 1 in that the composition does not contain Sargassum fusiforme extract in the raw materials, and the rest remains the same.
[0047] The whitening sunscreen composition of the present comparative example containing the Heteropappus altaicus contains the following raw materials in parts by weight: Heteropappus altaicus extract 23 parts, Acorus calamus extract 15 parts, Gentiana macrophylla extract 18 parts, Leontopodium alpinum extract 16 parts, Hippophae rhamnoides extract 15 parts, nicotinamide 8 parts, collagen 4 parts, sodium hyaluronate 1.5 parts, squalane 9 parts, decyl glucoside 2 parts, lactobacillus ferment 6 parts, ethylene glycol distearate 4 parts, sorbitan olivate 2 parts, Tween 80 6 parts, 1,3-propanediol 10 parts, and purified water 70 parts.
[0048] The compositions prepared in Examples 1-3 and Comparative Examples 1-6 above were subjected to performance tests for whitening sunscreen.
[0049] (1) Whitening effect test The whitening effect was evaluated in terms of the inhibitory effect of the composition on tyrosinase activity. 0.1 mL of the composition of Examples 1-3 and Comparative Examples 1-6 was placed in a test tube, and 100 μL of a phosphate buffer solution having a pH of 6.8 was added to the test tube and mixed to obtain a mixed solution as a sample solution group, and a phosphate buffer solution was used as a blank group. Human epidermal melanocyte B16 having a concentration of 1 x 10 5 was cultured in a 96-well plate for 24 h, and then the sample solution of the sample solution group and the phosphate buffer solution of the blank group were added and mixed in the dark, and incubated in a 37°C constant temperature water bath for 24 h. Then, 100 μL of a phosphate buffer solution containing 1% v / v of polyethylene glycol monooctylphenyl ether, and 50 μL of L-3,4-dihydroxyphenylalanine (L-DOPA) having a concentration of 0.2 mg / mL were added to each well, and finally the absorbance at 490 nm was measured after treatment at 37°C for 3 h, and the tyrosinase inhibition rate was calculated.
[0050] Tyrosinase inhibition rate (%) = [1 - (A sample solution group - A blank group)] x 100%.
[0051] Table 1 Inhibitory effect of tyrosinase activity
[0052] As can be seen from the data of Examples 1-3 and Comparative Examples 1-6 in Table 1, when the raw material component ratio in the composition is unreasonable, the inhibitory effect on tyrosinase activity is obviously reduced; when the raw material components of the composition do not contain the extract of Gerbera jamesonii, the extract of Acorus gramineus Soland and the extract of Sanguisorba officinalis, the tyrosinase inhibition rate shows a downward trend to varying degrees; changing the preparation method of the extract of Gerbera jamesonii or changing the preparation method of the extract of Acorus gramineus Soland also affects the tyrosinase inhibition rate. The above results show that the addition of the extract of Gerbera jamesonii, the extract of Acorus gramineus Soland and the extract of Sanguisorba officinalis in the composition promotes each other to a certain extent, which can improve the overall inhibitory effect of the composition on tyrosinase, reduce the transport of melanin to the stratum corneum, reduce the deposition of melanin on the surface of the skin, and thus achieve a good whitening effect.
[0053] (2) Sunscreen effect test The compositions prepared in Examples 1-3 and Comparative Examples 1-6 were respectively uniformly coated on PMMA plates (5 cm x 5 cm in size) at a density of 2 mg / cm 2 Then, SPF-290AS ultraviolet spectrophotometer was used for scanning test, and the SPF value was calculated. The results are shown in Table 2.
[0054] Table 2 Sunscreen effect test effect
[0055] As can be seen from the data of Examples 1-3 and Comparative Examples 1-6 in Table 2, the SPF value of Examples 1-3 is higher than that of Comparative Examples 1-6. Compared with Example 1, the different raw material ratios of the composition of Comparative Example 1 result in weakened synergistic effect of each component, and the SPF value decreases, indicating that mixing each raw material according to a specific ratio can promote the sunscreen effect of the composition. Compared with Example 1, the absence of the extract of Gerbera jamesonii in the raw material of Comparative Example 2, the absence of the extract of Acorus gramineus Soland in the raw material of Comparative Example 4 and the absence of the extract of Sanguisorba officinalis in the raw material of Comparative Example 6 also make the SPF value decrease, indicating that using the extract of Gerbera jamesonii, the extract of Acorus gramineus Soland and the extract of Sanguisorba officinalis as raw materials can improve the ultraviolet absorption capacity of the composition, reduce the penetration of ultraviolet into the skin, and improve the sunscreen effect. And it can reduce the irritability of ultraviolet, reduce water loss, enhance the skin barrier function, and enhance the resistance to external stimulation. As can be seen from Examples 1, Comparative Example 3 and Comparative Example 5, using appropriate strains to ferment the extract of Gerbera jamesonii and the extract of Acorus gramineus Soland can make the effective components more easily dissolved and improve the activity of the active components, thereby enhancing the sunscreen effect.
[0056] The above merely provides the preferred embodiment of the present application, and is not used to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims
1. A whitening sunscreen composition comprising a red gill-ear chrysanthemum, characterized in that, The ingredients include the following parts by weight: 17-20 parts of *Hypericum rubrum* extract, 10-13 parts of *Acorus calamus* extract, 12-16 parts of *Gentiana macrophylla* extract, 13-15 parts of *Edelweiss* extract, 11-14 parts of *Lygodium japonicum* extract, 13-15 parts of *Hippophae rhamnoides* extract, 5-8 parts of nicotinamide, 4-7 parts of collagen, 1.5-3 parts of sodium hyaluronate, 4-9 parts of squalane, 1-3 parts of decyl glucoside, 6-9 parts of *Lactobacillus* fermentation product, 3-5 parts of ethylene glycol distearate, 2-3 parts of sorbitan olive oil ester, 4-6 parts of Tween 80, 9-12 parts of 1,3-propanediol, and 70-80 parts of water.
2. The whitening sunscreen composition containing Rubus coreanus according to claim 1, wherein the Rubus coreanus is in the form of a powder having a particle size of 100 mesh or less. The extract of *Hypericum rubrum* is obtained by fermenting *Hypericum rubrum* with *Aspergillus cristatum*.
3. The whitening sunscreen composition containing Rubus coreanus of claim 2, wherein the Rubus coreanus is extracted from Rubus coreanus S. Lee. The preparation method of the extract of *Hypericum rubrum* is as follows: *Hypericum rubrum* is pulverized into powder, then activated *Eurotium cristatum* bacterial suspension and culture medium are added and stirred evenly, fermented and cultured, sterilized to obtain crude fermentation broth, then the crude fermentation broth is centrifuged, then the precipitate and bacterial cells are discarded, the liquid is concentrated to obtain *Hypericum rubrum* extract.
4. The whitening sunscreen composition containing Rubus coreanus according to claim 3, wherein the Rubus coreanus is in the form of a powder having a particle size of 100 mesh or less. The powder, Eurotium amstelodami bacterial suspension and culture medium mass ratio is 23-27:0.1-0.2:100; the concentration of the Eurotium amstelodami bacterial suspension is 10 5 CFU / mL. 6 CFU / mL.
5. The whitening sunscreen composition containing Rubus coreanus according to claim 3, wherein the Rubus coreanus is in the form of a powder having a particle size of 100 mesh or less. The fermentation culture is carried out at a temperature of 28-30℃, a rotation speed of 120-150 r / min, and a time of 95-100 h; the centrifugation is carried out at a rotation speed of 1200-1500 r / min and a time of 10-12 min.
6. The whitening sunscreen composition containing Ainsliaea henryi according to claim 1, wherein the composition is a cosmetic composition. The preparation method of the Tibetan calamus extract is as follows: Tibetan calamus is crushed into powder and mixed with water to obtain a mixture. A fermentation bacteria suspension is added to the mixture and mixed well before fermentation. After sterilization, a fermentation liquid is obtained. The fermentation bacteria are centrifuged, and then the precipitate and bacterial cells are discarded. The liquid is concentrated to obtain the Tibetan calamus extract.
7. The whitening and sunscreen composition containing *Clerodendrum trichotomum* according to claim 6, characterized in that, The ratio of calamus powder to water in the mixture is 1:9~12 (g / mL); the amount of fermentation bacteria suspension added is 4~6% v / v of the mixture; the fermentation temperature is 30~32℃, the rotation speed is 140~160 r / min, and the time is 48~72h.
8. The whitening and sunscreen composition containing *Clerodendrum trichotomum* according to claim 6, characterized in that, The strains in the fermentation bacterial suspension include 4-6:8-10 of Malassezia furfur and Lactobacillus rhamnosus by mass ratio; the concentration of the Malassezia furfur is 10 6 ~10 7 CFU / mL; and the concentration of the Lactobacillus rhamnosus is 10 5 ~10 7 CFU / mL.
9. A method for preparing the whitening and sunscreen composition containing *Lysimachia christinae* according to any one of claims 1-8, characterized in that, Includes the following steps: (1) Squalane, ethylene glycol distearate and sorbitan olive oil ester are stirred and mixed to obtain a mixture; (2) Mix Tween 80, 1,3-propanediol and water, then add red tassel extract, Tibetan calamus extract, gentian extract, alpine edelweiss extract, lygodium extract, sea buckthorn extract, nicotinamide, collagen and sodium hyaluronate and mix again. Finally, add the mixture from step (1) and mix to obtain the target whitening sunscreen composition.
10. The method for preparing the whitening and sunscreen composition containing *Lysimachia christinae* according to claim 9, characterized in that, In step (1), the stirring speed is 150~180r / min, the temperature is 60~70℃, and the time is 8~10min; in step (2), the stirring speed is 200~320r / min, the temperature is 60~65℃, and the time is 15~20min.