Nutritional supplement for pregnant pets and preparation method of nutritional supplement
By using chickpea enzymatic hydrolysis to generate GABA and amino acid chelated calcium, combined with egg yolk powder and lactoferrin encapsulation technology, the problem of insufficient mineral absorption and progesterone deficiency in prenatal pet supplements is solved, achieving comprehensive nutritional support and mood regulation, and improving the health of both mother and fetus.
Patent Information
- Application Number
- CN202512022321.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-30
- Publication Date
- 2026-03-06
AI Technical Summary
Mineral absorption and utilization rates in pet supplements during pregnancy are low, iron, calcium, and zinc compete for absorption sites, progesterone levels are insufficient, existing products do not take into account the needs of mood regulation and intestinal health, and there is no synergistic effect or mutual interference between ingredients.
By enzymatically hydrolyzing chickpeas to generate GABA and amino acid chelated calcium, and combining this with egg yolk powder and lactoferrin encapsulation technology, a nutritional supplement for pregnant pets is prepared to improve mineral absorption and utilization, alleviate anxiety, support progesterone secretion, and provide comprehensive nutrition.
It improves the bioavailability of minerals and nutrients, ensures stable absorption, reduces intestinal irritation, meets the nutritional and mood regulation needs during pregnancy, and protects the health of the mother and fetus.
Smart Images
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of pet food, specifically relating to a nutritional supplement for pregnant pets and its preparation method. Background Technology
[0002] Pregnant pets need to supplement with minerals such as calcium, iron, and zinc, as well as a large amount of protein. However, traditional supplements often directly add inorganic calcium, ferrous fumarate, zinc gluconate, etc. These three substances are prone to forming insoluble complexes in the intestine (such as iron-calcium phosphate and zinc-calcium precipitate), resulting in an absorption and utilization rate of less than 30%, which cannot meet the needs of efficient supplementation during pregnancy. Furthermore, iron, calcium, and zinc compete for intestinal absorption sites and inhibit each other's absorption.
[0003] Progesterone, primarily secreted by the corpus luteum of the ovary and the placenta, is a crucial hormone for maintaining pregnancy and is often referred to as the "fertility hormone," acting as the "lifeline" for pregnancy. Its main functions include: thickening the uterine lining to create a suitable environment for implantation and growth of the fertilized egg; preventing uterine contractions to provide a quiet and stable environment for fetal growth and prevent miscarriage; and closing the cervix to prevent bacterial invasion and protect the fetus. It also plays a role in immune regulation and promotes breast development. Insufficient progesterone levels during pregnancy can lead to a series of risks, such as difficulty in embryo implantation, early miscarriage, or threatened miscarriage. Malnutrition and excessive anxiety and stress can both cause low progesterone levels. Anxiety can affect the function of the hypothalamus-pituitary-ovarian axis, leading to endocrine disorders and consequently low progesterone levels.
[0004] Therefore, in addition to supplementing with healthy fats, vitamins, and minerals such as zinc to regulate progesterone levels during pregnancy, it is also crucial to manage emotions and maintain adequate progesterone levels. Currently, most products focus only on replenishing blood and providing various nutrients, neglecting the needs for emotional regulation and gut health during pregnancy; some products, while containing multiple nutrients, lack synergistic effects between their components, and may even interfere with each other. Summary of the Invention
[0005] The technical problem to be solved: Processing chickpeas to enrich them with GABA can alleviate anxiety and other emotional issues, thereby indirectly affecting progesterone balance. Simultaneously, amino acid chelated calcium is synthesized during GABA production. This solution addresses the antagonistic relationship between mineral absorption through the generation of amino acid chelated calcium and the encapsulation of functional substances. Egg yolk powder and lactoferrin are used to encapsulate GABA and amino acid chelated calcium, achieving a slow-release effect while supplementing nutrients, avoiding reactions with the ferrous fumarate supplemented in the product, and improving bioavailability. This product provides comprehensive nutrition, directly or indirectly supporting progesterone secretion, while also providing abundant minerals and phospholipids, enhancing maternal health and ensuring normal fetal development.
[0006] Technical solution: A method for preparing a nutritional supplement for pregnant pets, comprising the following steps: S1. Enzymatic hydrolysis of chickpeas: Sieve chickpea flour, add water to make a slurry, adjust the pH with citric acid, add protease, and hydrolyze at a constant temperature to obtain the enzymatically hydrolyzed slurry. S2. Enrichment of GABA: After adding GAD enzyme to the enzymatically hydrolyzed slurry and stirring evenly, calcium chloride is slowly added in batches and stirred in a water bath. After the reaction is completed, the enzyme is inactivated by heating and homogenized to obtain a chickpea flour mixture. S3. Oil phase preparation: Egg yolk powder is added to an ethanol solution and stirred until dissolved to obtain an egg yolk powder ethanol solution. Fat-soluble vitamins are added to flaxseed oil and stirred until dissolved to obtain a flaxseed oil mixture. This mixture is then mixed with the egg yolk powder ethanol solution and sonicated to obtain an oil phase mixture. S4. Emulsion preparation: The above oil phase mixture is slowly added to the chickpea flour mixture, homogenized to form a primary emulsion, then lactoferrin and bee pollen mixture is added, pH is adjusted, homogenized again, and spray-dried to obtain the functional ingredient. S5. Basic raw material pretreatment: thaw chicken breast and tuna, steam chicken breast, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve and compound taurine, ferrous fumarate, vitamin C, L-carnitine and folic acid to form excipients. S6. Mixing and molding: Raw materials, auxiliary materials and functional ingredients are fed into a chopper in batches and mixed. The mixture is then filled and sterilized to obtain a nutritional supplement for pregnant pets.
[0007] Furthermore, in step S1, the mass ratio of chickpea flour to water is (1-2):(6-8); the pH is adjusted to 5.2-5.5; the amount of protease added to the slurry is 3000-5000 U / g; the constant temperature is 45-55℃; the stirring speed is 200-500 rpm; and the time is 1-2 hours.
[0008] Furthermore, in step S2, the amount of GAD enzyme added to the enzymatically hydrolyzed slurry is 1000-3000 U / g; the batching is done in 3 batches, with an interval of 15-25 min between each batch; the water bath stirring temperature is 30-50℃, and the time is 1.5-2.5 h; the amount of calcium chloride added is 3-5 wt.% of the chickpea flour mass; the temperature for enzyme inactivation is 65-85℃, and the time is 20-30 min; the homogenization speed is 2000-5000 rpm, and the time is 5-10 min.
[0009] Furthermore, in step S3, the concentration of the ethanol solution is 70-80 wt.%; the mass ratio of egg yolk powder to ethanol solution is 1:(5-9); the stirring speed is 400-600 rpm, and the time is 20-40 min; the fat-soluble vitamins are vitamin A, vitamin D3, and vitamin E; the amount of fat-soluble vitamins added is 0.1-0.3 wt.% of the mass of flaxseed oil; the mass ratio of the flaxseed oil mixture to the egg yolk powder ethanol solution is 1:(1-3); the ultrasonic power is 200-500 W, and the ultrasonic time is 10-20 min.
[0010] Furthermore, the mass ratio of vitamin A, vitamin D3, and vitamin E is (5-6):(2-3):(25-30).
[0011] Furthermore, in step S4, the mass ratio of the oil phase mixture, chickpea flour mixture, lactoferrin, and bee pollen mixture is (1-2):1:1; the homogenization speed is 8000-10000 rpm, and the time is 10-20 min; the lactoferrin content in the lactoferrin and bee pollen mixture is 2-3 wt.%, and the bee pollen content is 1-2 wt.%; the pH is adjusted to 6.4-6.6; the homogenization speed is 9000-12000 rpm, and the time is 10-20 min.
[0012] Furthermore, the cooking time in step S5 is 25-30 minutes; the auxiliary materials contain 1 wt.% taurine, 0.5 wt.% ferrous fumarate, 0.5 wt.% vitamin C, 0.15 wt.% L-carnitine, and 0.1 wt.% folic acid.
[0013] Furthermore, in step S6, the raw materials include 40-50 wt.% chicken breast, 15-20 wt.% tuna, 5-10 wt.% chicken liver, 2-5 wt.% chicken oil, 1-2 wt.% fish oil, 0.3-0.5 wt.% angelica, 0.5-0.7 wt.% ginseng, 0.4-0.6 wt.% astragalus, and the remainder is water; the mass ratio of raw materials, excipients and functional ingredients is (80-90):(15-20):(10-20); the sterilization temperature is 121℃ and the time is 30-50 min.
[0014] The above-described preparation method yields a nutritional supplement for pregnant pets.
[0015] Beneficial effects: In this product, the plant estrogens such as soy isoflavones from legumes can increase serum progesterone levels after conception. The GABA produced after processing can alleviate anxiety and other mood swings, thus indirectly affecting progesterone balance. Furthermore, this product also includes flaxseed oil rich in Omega-3 fatty acids, chicken rich in vitamin B6, egg yolk powder rich in zinc and lecithin, as well as lactoferrin, folic acid, and other substances. These substances directly or indirectly support progesterone secretion by participating in steroid hormone synthesis and regulating ovarian function, while also providing rich nutrition and having a calming effect on the fetus.
[0016] This invention simultaneously meets three major needs: calcium, iron, and zinc supplementation, mood regulation, and blood replenishment for a healthy pregnancy. It not only regulates the balance of progesterone in the body to meet the mother's needs for progesterone and blood replenishment, but is also rich in various minerals and proteins, taking into account fetal development. The synergistic effect of various nutrients not only enhances the mother's immunity and reduces maternal pregnancy complications, but also ensures normal fetal development and reduces the risk of growth retardation and malformation.
[0017] This invention activates GAD enzyme with calcium ions, efficiently converting glutamic acid produced by the enzymatic hydrolysis of chickpeas into GABA, providing pets with calming and anti-anxiety functional factors. It considers both prenatal nutrition and the stability of mood during pregnancy. Simultaneously, other amino acids produced by enzymatic hydrolysis are chelated in situ with calcium ions to generate amino acid chelated calcium. Its absorption pathway differs from traditional calcium supplements, resulting in higher bioavailability, a smooth and slow absorption process, less intestinal irritation, and minimal direct interaction with iron. Unreacted peptides and amino acids provide a good matrix for the absorption of other nutrients, greatly improving raw material utilization. The production process is efficient and intensive, yielding two high-value functional components—GABA and amino acid chelated calcium.
[0018] This invention uses amino acid chelated calcium instead of ordinary calcium ions, and encapsulates the amino acid chelated calcium to prevent adverse reactions with other components during processing and storage, and achieves sustained release, further reducing its concentration when it encounters iron ions instantaneously in the intestine. Furthermore, egg yolk powder and lactoferrin are used as wall materials to protect the core functional substance, ensuring that GABA can safely reach the small intestine for release and absorption, and effectively masking the special odor of GABA itself, thus improving the palatability of the product.
[0019] This invention fully utilizes the natural properties of the inherent ingredients in the formula without introducing unnecessary chemically synthesized excipients. The egg yolk powder contains lecithin, which acts as an emulsifier, and protein, which acts as an embedding wall material. It also provides various nutrients such as phospholipids and zinc. Lactoferrin acts as an embedding wall material, as well as an iron carrier and absorption promoter. The lecithin and peptides contained in the chickpea hydrolysate also participate in the formation of the emulsion system. This simplifies the production process, reduces costs, ensures the clean label of the formula, and is more in line with the trend of natural and healthy pet food.
[0020] The dual emulsion structure of this invention allows the aqueous phase to dissolve GABA, amino acid chelated calcium, peptides, etc., while the oil phase can dissolve fat-soluble vitamin D and vitamin E. This not only protects the GABA in the aqueous phase and the unsaturated fatty acids (such as ALA in flaxseed oil) in the oil phase from oxidation and rancidity and helps with vitamin absorption, but also provides strong physical and chemical stability, ensuring the stability of the final product.
[0021] In addition to high-quality protein, fat, and fatty acids such as flaxseed oil, ferrous fumarate, chicken breast, tuna, chicken liver, chicken oil, and fish oil, this formula also incorporates traditional nourishing herbs such as angelica, ginseng, and astragalus. It aims to regulate qi and blood, enhance immunity, and combat fatigue, which aligns with the needs of pregnant mothers to cope with the enormous physiological pressure. Bee pollen is rich in various nutrients and active substances, providing comprehensive and high-density nutritional support for pregnant mothers and fetuses. Detailed Implementation
[0022] The following embodiments further describe the present invention. These embodiments are illustrative of the present invention, but the present invention is not limited to these embodiments: Example 1
[0023] A nutritional supplement for pregnant pets and its preparation method: S1. Enzymatic hydrolysis of chickpeas: 100g of chickpea flour that has passed through an 80-mesh sieve is mixed with 300g of water to make a slurry. The pH is adjusted to 5.2 with citric acid, and protease is added to make the protease activity reach 3000U / g. The mixture is stirred at 200 rpm for 1.5h at a constant temperature of 45℃ to obtain the enzymatically hydrolyzed slurry. S2. GABA enrichment: GAD enzyme was added to the enzymatically hydrolyzed slurry to make the protease activity reach 3000U / g. After stirring evenly, 3g of calcium chloride was dissolved and added slowly in 3 batches, with an interval of 15min between each batch. The reaction was carried out in a 30℃ water bath for 2h with stirring. After the reaction was completed, the temperature was raised to 65℃ and kept at 20min to inactivate the enzyme. The mixture was homogenized at 5000rpm for 5min to obtain chickpea flour mixture. S3. Oil phase preparation: 35g of egg yolk powder was added to 175g of 70wt.% ethanol solution and stirred at 400 rpm for 20min until dissolved to obtain egg yolk powder ethanol solution. 0.05g of vitamin A, 0.03g of vitamin D3, and 0.25g of vitamin E were added to 99.67g of flaxseed oil and stirred until dissolved to obtain flaxseed oil mixture. This mixture was then mixed with the egg yolk powder ethanol solution and sonicated at 300W for 20min to obtain oil phase mixture. S4. Emulsion preparation: Slowly add the above 300g oil phase mixture to 150g chickpea flour mixture, homogenize to form a primary emulsion, then add 150g of lactoferrin and bee pollen mixture solution, wherein the lactoferrin content is 2wt.% and the bee pollen content is 1wt.%, adjust the pH to 6.4, homogenize again at 9000 rpm for 15min, and then spray dry to obtain the functional ingredient; S5. Basic raw material pretreatment: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 97.75 water to prepare excipients; S6. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of auxiliary materials and 100g of functional ingredients are added to a chopper in batches and mixed. The mixture is then filled and sterilized at 121℃ for 30 minutes to obtain a nutritional supplement for pregnant pets. Example 2
[0024] A nutritional supplement for pregnant pets and its preparation method: S1. Enzymatic hydrolysis of chickpeas: 100g of chickpea flour that has passed through an 80-mesh sieve is mixed with 300g of water to make a slurry. The pH is adjusted to 5.2 with citric acid, and protease is added to make the protease activity reach 3000U / g. The mixture is stirred at 200 rpm for 1.5h at a constant temperature of 45℃ to obtain the enzymatically hydrolyzed slurry. S2. GABA enrichment: GAD enzyme was added to the enzymatically hydrolyzed slurry to make the protease activity reach 3000U / g. After stirring evenly, 3g of calcium chloride was dissolved and added slowly in 3 batches, with an interval of 15min between each batch. The reaction was carried out in a 30℃ water bath for 2h with stirring. After the reaction was completed, the temperature was raised to 65℃ and kept at 20min to inactivate the enzyme. The mixture was homogenized at 5000rpm for 5min to obtain chickpea flour mixture. S3. Oil phase preparation: 35g of egg yolk powder was added to 175g of 70wt.% ethanol solution and stirred at 400 rpm for 20min until dissolved to obtain egg yolk powder ethanol solution. 0.05g of vitamin A, 0.03g of vitamin D3, and 0.25g of vitamin E were added to 99.67g of flaxseed oil and stirred until dissolved to obtain flaxseed oil mixture. This mixture was then mixed with the egg yolk powder ethanol solution and sonicated at 300W for 20min to obtain oil phase mixture. S4. Emulsion preparation: Slowly add 150g of the above oil phase mixture to 150g of chickpea flour mixture, homogenize to form a primary emulsion, then add 150g of a mixture of lactoferrin and bee pollen, wherein the lactoferrin content is 2wt.% and the bee pollen content is 1wt.%, adjust the pH to 6.4, homogenize again at 9000 rpm for 15min, and then spray dry to obtain the functional ingredient; S5. Basic raw material pretreatment: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 97.75 water to prepare excipients; S6. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of auxiliary materials and 100g of functional ingredients are added to a chopper in batches and mixed. The mixture is then filled and sterilized at 121℃ for 30 minutes to obtain a nutritional supplement for pregnant pets. Example 3
[0025] A nutritional supplement for pregnant pets and its preparation method: S1. Enzymatic hydrolysis of chickpeas: 100g of chickpea flour that has passed through an 80-mesh sieve is mixed with 300g of water to make a slurry. The pH is adjusted to 5.2 with citric acid, and protease is added to make the protease activity reach 3000U / g. The mixture is stirred at 200 rpm for 1.5h at a constant temperature of 45℃ to obtain the enzymatically hydrolyzed slurry. S2. GABA enrichment: GAD enzyme was added to the enzymatically hydrolyzed slurry to make the protease activity reach 3000U / g. After stirring evenly, 3g of calcium chloride was dissolved and added slowly in 3 batches, with an interval of 15min between each batch. The reaction was carried out in a 30℃ water bath for 2h with stirring. After the reaction was completed, the temperature was raised to 65℃ and kept at 20min to inactivate the enzyme. The mixture was homogenized at 5000rpm for 5min to obtain chickpea flour mixture. S3. Oil phase preparation: 50g of egg yolk powder was added to 250g of 70wt.% ethanol solution and stirred at 400 rpm for 20min until dissolved to obtain egg yolk powder ethanol solution. 0.05g of vitamin A, 0.03g of vitamin D3, and 0.25g of vitamin E were added to 99.67g of flaxseed oil and stirred until dissolved to obtain flaxseed oil mixture. This mixture was then mixed with the egg yolk powder ethanol solution and sonicated at 300W for 20min to obtain oil phase mixture. S4. Emulsion preparation: Slowly add the above 300g oil phase mixture to 150g chickpea flour mixture, homogenize to form a primary emulsion, then add 150g of lactoferrin and bee pollen mixture solution, wherein the lactoferrin content is 2wt.% and the bee pollen content is 1wt.%, adjust the pH to 6.4, homogenize again at 9000 rpm for 15min, and then spray dry to obtain the functional ingredient; S5. Basic raw material pretreatment: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 97.75 water to prepare excipients; S6. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of auxiliary materials and 100g of functional ingredients are added to a chopper in batches and mixed. The mixture is then filled and sterilized at 121℃ for 30 minutes to obtain a nutritional supplement for pregnant pets. Example 4
[0026] A nutritional supplement for pregnant pets and its preparation method: S1. Enzymatic hydrolysis of chickpeas: 100g of chickpea flour that has passed through an 80-mesh sieve is mixed with 300g of water to make a slurry. The pH is adjusted to 5.2 with citric acid, and protease is added to make the protease activity reach 3000U / g. The mixture is stirred at 200 rpm for 1.5h at a constant temperature of 45℃ to obtain the enzymatically hydrolyzed slurry. S2. GABA enrichment: GAD enzyme was added to the enzymatically hydrolyzed slurry to make the protease activity reach 3000 U / g. After stirring evenly, 4.5g of calcium chloride was dissolved and added slowly in 3 batches, with an interval of 15 min between each batch. The mixture was stirred in a 30℃ water bath for 2 hours. After the reaction was completed, the temperature was raised to 65℃ and kept at 20 min to inactivate the enzyme. The mixture was homogenized at 5000 rpm for 5 min to obtain a chickpea flour mixture. S3. Oil phase preparation: 35g of egg yolk powder was added to 175g of 70wt.% ethanol solution and stirred at 400 rpm for 20min until dissolved to obtain egg yolk powder ethanol solution. 0.05g of vitamin A, 0.03g of vitamin D3, and 0.25g of vitamin E were added to 99.67g of flaxseed oil and stirred until dissolved to obtain flaxseed oil mixture. This mixture was then mixed with the egg yolk powder ethanol solution and sonicated at 300W for 20min to obtain oil phase mixture. S4. Emulsion preparation: Slowly add the above 300g oil phase mixture to 150g chickpea flour mixture, homogenize to form a primary emulsion, then add 150g of lactoferrin and bee pollen mixture solution, wherein the lactoferrin content is 2wt.% and the bee pollen content is 1wt.%, adjust the pH to 6.4, homogenize again at 9000 rpm for 15min, and then spray dry to obtain the functional ingredient; S5. Basic raw material pretreatment: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 97.75 water to prepare excipients; S6. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of auxiliary materials and 100g of functional ingredients are added to a chopper in batches and mixed. The mixture is then filled and sterilized at 121℃ for 30 minutes to obtain a nutritional supplement for pregnant pets. Example 5
[0027] A nutritional supplement for pregnant pets and its preparation method: S1. Enzymatic hydrolysis of chickpeas: 100g of chickpea flour that has passed through an 80-mesh sieve is mixed with 600g of water to make a slurry. The pH is adjusted to 5.2 with citric acid, and protease is added to make the protease activity reach 3000U / g. The mixture is stirred at 200 rpm for 1.5h at a constant temperature of 45℃ to obtain the enzymatically hydrolyzed slurry. S2. GABA enrichment: GAD enzyme was added to the enzymatically hydrolyzed slurry to make the protease activity reach 3000U / g. After stirring evenly, 3g of calcium chloride was dissolved and added slowly in 3 batches, with an interval of 15min between each batch. The reaction was carried out in a 30℃ water bath for 2h with stirring. After the reaction was completed, the temperature was raised to 65℃ and kept at 20min to inactivate the enzyme. The mixture was homogenized at 5000rpm for 5min to obtain chickpea flour mixture. S3. Oil phase preparation: 35g of egg yolk powder was added to 175g of 70wt.% ethanol solution and stirred at 400 rpm for 20min until dissolved to obtain egg yolk powder ethanol solution. 0.05g of vitamin A, 0.03g of vitamin D3, and 0.25g of vitamin E were added to 99.67g of flaxseed oil and stirred until dissolved to obtain flaxseed oil mixture. This mixture was then mixed with the egg yolk powder ethanol solution and sonicated at 300W for 20min to obtain oil phase mixture. S4. Emulsion preparation: Slowly add the above 300g oil phase mixture to 150g chickpea flour mixture, homogenize to form a primary emulsion, then add 150g of lactoferrin and bee pollen mixture solution, wherein the lactoferrin content is 2wt.% and the bee pollen content is 1wt.%, adjust the pH to 6.4, homogenize again at 9000 rpm for 15min, and then spray dry to obtain the functional ingredient; S5. Basic raw material pretreatment: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 97.75 water to prepare excipients; S6. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of auxiliary materials and 100g of functional ingredients are added to a chopper in batches and mixed. The mixture is then filled and sterilized at 121℃ for 30 minutes to obtain a nutritional supplement for pregnant pets. Example 6
[0028] A nutritional supplement for pregnant pets and its preparation method: S1. Enzymatic hydrolysis of chickpeas: 100g of chickpea flour that has passed through an 80-mesh sieve is mixed with 300g of water to make a slurry. The pH is adjusted to 5.2 with citric acid, and protease is added to make the protease activity reach 3000U / g. The mixture is stirred at 200 rpm for 1.5h at a constant temperature of 45℃ to obtain the enzymatically hydrolyzed slurry. S2. GABA enrichment: GAD enzyme was added to the enzymatically hydrolyzed slurry to make the protease activity reach 3000U / g. After stirring evenly, 3g of calcium chloride was dissolved and added slowly in 3 batches, with an interval of 15min between each batch. The reaction was carried out in a 30℃ water bath for 2h with stirring. After the reaction was completed, the temperature was raised to 65℃ and kept at 20min to inactivate the enzyme. The mixture was homogenized at 5000rpm for 5min to obtain chickpea flour mixture. S3. Oil phase preparation: 35g of egg yolk powder was added to 175g of 70wt.% ethanol solution and stirred at 400 rpm for 20min until dissolved to obtain egg yolk powder ethanol solution. 0.05g of vitamin A, 0.03g of vitamin D3, and 0.25g of vitamin E were added to 99.67g of flaxseed oil and stirred until dissolved to obtain flaxseed oil mixture. This mixture was then mixed with the egg yolk powder ethanol solution and sonicated at 300W for 20min to obtain oil phase mixture. S4. Emulsion preparation: Slowly add the above 300g oil phase mixture to 150g chickpea flour mixture, homogenize to form a primary emulsion, then add 150g of lactoferrin and bee pollen mixture solution, wherein the lactoferrin content is 2wt.% and the bee pollen content is 1wt.%, adjust the pH to 6.4, homogenize again at 9000 rpm for 15min, and then spray dry to obtain the functional ingredient; S5. Basic raw material pretreatment: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 97.75 water to prepare excipients; S6. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of auxiliary materials and 100g of functional ingredients are added to a chopper in batches and mixed. The mixture is then filled and sterilized at 121℃ for 30 minutes to obtain a nutritional supplement for pregnant pets. Comparative Example 1
[0029] The difference between this comparative example and Example 1 is that the chickpeas are not enzymatically hydrolyzed, as detailed below: S1. Enrichment of GABA: 100g of chickpea flour that has passed through an 80-mesh sieve is mixed with 300g of water to form a slurry. The pH is adjusted to 5.2 with citric acid. GAD enzyme is added to make the protease activity reach 3000U / g. After stirring evenly, 3g of calcium chloride is dissolved and added slowly in 3 batches with an interval of 15min between each batch. The mixture is stirred in a 30℃ water bath for 2h. After the reaction is completed, the temperature is raised to 65℃ and kept at 20min to inactivate the enzyme. The mixture is homogenized at 5000rpm for 5min to obtain a chickpea flour mixture. S2. Oil phase preparation: 35g of egg yolk powder was added to 175g of 70wt.% ethanol solution and stirred at 400 rpm for 20min until dissolved to obtain egg yolk powder ethanol solution. 0.05g of vitamin A, 0.03g of vitamin D3, and 0.25g of vitamin E were added to 99.67g of flaxseed oil and stirred until dissolved to obtain flaxseed oil mixture. This mixture was then mixed with the egg yolk powder ethanol solution and sonicated at 300W for 20min to obtain oil phase mixture. S3. Emulsion preparation: Slowly add the above 300g oil phase mixture to 150g chickpea flour mixture, homogenize to form a primary emulsion, then add 150g of lactoferrin and bee pollen mixture, wherein the lactoferrin content is 2wt.% and the bee pollen content is 1wt.%, adjust the pH to 6.4, homogenize again at 9000 rpm for 15min, and then spray dry to obtain the functional ingredient; S4. Basic raw material pretreatment: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 97.75 water to prepare excipients; S5. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of auxiliary materials and 100g of functional ingredients are added to a chopper in batches and mixed. The mixture is then filled and sterilized at 121℃ for 30 minutes to obtain a nutritional supplement for pregnant pets. Comparative Example 2
[0030] The difference between this comparative example and Example 1 is that it does not enrich GABA, as detailed below: S1. Enzymatic hydrolysis of chickpeas: 100g of chickpea flour that has passed through an 80-mesh sieve is mixed with 300g of water to make a slurry. The pH is adjusted to 5.2 with citric acid, and protease is added to make the protease activity reach 3000U / g. The mixture is stirred at 200 rpm for 1.5h at a constant temperature of 45℃ to obtain the enzymatically hydrolyzed slurry. S2. Adding calcium: Dissolve 3g of calcium chloride and slowly add it to the enzymatically hydrolyzed slurry in 3 batches, with an interval of 15min between each batch. Stir the reaction in a 30℃ water bath for 2h. After the reaction is completed, raise the temperature to 65℃ and keep it at that temperature for 20min to inactivate the enzyme. Homogenize at 5000rpm for 5min to obtain a chickpea flour mixture. S3. Oil phase preparation: 35g of egg yolk powder was added to 175g of 70wt.% ethanol solution and stirred at 400 rpm for 20min until dissolved to obtain egg yolk powder ethanol solution. 0.05g of vitamin A, 0.03g of vitamin D3, and 0.25g of vitamin E were added to 99.67g of flaxseed oil and stirred until dissolved to obtain flaxseed oil mixture. This mixture was then mixed with the egg yolk powder ethanol solution and sonicated at 300W for 20min to obtain oil phase mixture. S4. Emulsion preparation: Slowly add the above 300g oil phase mixture to 150g chickpea flour mixture, homogenize to form a primary emulsion, then add 150g of lactoferrin and bee pollen mixture solution, wherein the lactoferrin content is 2wt.% and the bee pollen content is 1wt.%, adjust the pH to 6.4, homogenize again at 9000 rpm for 15min, and then spray dry to obtain the functional ingredient; S5. Basic raw material pretreatment: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 97.75 water to prepare excipients; S6. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of auxiliary materials and 100g of functional ingredients are added to a chopper in batches and mixed. The mixture is then filled and sterilized at 121℃ for 30 minutes to obtain a nutritional supplement for pregnant pets. Comparative Example 3
[0031] The difference between this comparative example and Example 1 is that no emulsion is prepared, as detailed below: S1. Enzymatic hydrolysis of chickpeas: 100g of chickpea flour that has passed through an 80-mesh sieve is mixed with 300g of water to make a slurry. The pH is adjusted to 5.2 with citric acid, and protease is added to make the protease activity reach 3000U / g. The mixture is stirred at 200 rpm for 1.5h at a constant temperature of 45℃ to obtain the enzymatically hydrolyzed slurry. S2. GABA enrichment: GAD enzyme was added to the enzymatically hydrolyzed slurry to make the protease activity reach 3000U / g. After stirring evenly, 3g of calcium chloride was dissolved and added slowly in 3 batches, with an interval of 15min between each batch. The reaction was carried out in a 30℃ water bath for 2h with stirring. After the reaction was completed, the temperature was raised to 65℃ and kept at 20min to inactivate the enzyme. The mixture was homogenized at 5000rpm for 5min to obtain chickpea flour mixture. S3. Preparation of functional ingredients: Add 35g of egg yolk powder to 175g of water and stir at 400 rpm for 20 minutes until dissolved to obtain an egg yolk powder mixture. Slowly add the mixture to 150g of chickpea flour mixture and homogenize. Then add 150g of a mixture of lactoferrin and bee pollen, with lactoferrin content of 2wt.% and bee pollen content of 1wt.%. Adjust the pH to 6.4, homogenize again at 9000 rpm for 15 minutes, and then spray dry to obtain the functional ingredients. S5. Basic raw material pretreatment: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 97.75g water to prepare excipients, add 0.05g vitamin A, 0.03g vitamin D3 and 0.25g vitamin E to 99.67g flaxseed oil and stir to dissolve to obtain flaxseed oil mixture; S6. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of excipients, 4g of flaxseed oil mixture and 100g of functional ingredients are added to a chopper in batches and chopped. The mixture is then filled and sterilized at 121℃ for 30 minutes to obtain the nutritional supplement for pregnant pets. Comparative Example 4
[0032] The difference between this comparative example and Example 1 is that the chickpea flour mixture is added directly as an ingredient, as detailed below: S1. Enzymatic hydrolysis of chickpeas: 100g of chickpea flour that has passed through an 80-mesh sieve is mixed with 300g of water to make a slurry. The pH is adjusted to 5.2 with citric acid, and protease is added to make the protease activity reach 3000U / g. The mixture is stirred at 200 rpm for 1.5h at a constant temperature of 45℃ to obtain the enzymatically hydrolyzed slurry. S2. GABA enrichment: GAD enzyme was added to the enzymatically hydrolyzed slurry to make the protease activity reach 3000 U / g. After stirring evenly, 3g of calcium chloride was dissolved and added slowly in 3 batches, with an interval of 15 min between each batch. The reaction was carried out in a 30℃ water bath with stirring for 2 hours. After the reaction was completed, the temperature was raised to 65℃ and kept at 20 min to inactivate the enzyme. The mixture was homogenized at 5000 rpm for 5 min to obtain a chickpea flour mixture. The mixture was then spray-dried to obtain the enzyme-treated chickpea flour. S3. Pretreatment of basic raw materials: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 97.75g water to prepare excipients, add 0.05g vitamin A, 0.03g vitamin D3 and 0.25g vitamin E to 99.67g flaxseed oil and stir to dissolve to obtain flaxseed oil mixture; S4. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of auxiliary materials, 4g of flaxseed oil mixture, 40g of enzyme-treated chickpea flour, 35g of egg yolk powder, 3g of lactoferrin, and 1.5g of bee pollen are added to a chopper in batches and chopped. The mixture is then filled into containers and sterilized at 121℃ for 30 minutes to obtain a nutritional supplement for pregnant pets. Comparative Example 5
[0033] The difference between this comparative example and Example 1 is that calcium and zinc salts are added directly, as detailed below: S1. Enzymatic hydrolysis of chickpeas: 100g of chickpea flour that has passed through an 80-mesh sieve is mixed with 300g of water to make a slurry. The pH is adjusted to 5.2 with citric acid, and protease is added to make the protease activity reach 3000U / g. The mixture is stirred at 200 rpm for 1.5h at a constant temperature of 45℃ to obtain the enzymatically hydrolyzed slurry. S2. Enrichment of GABA: GAD enzyme was added to the hydrolyzed slurry to make the protease activity reach 3000 U / g. After stirring evenly, the pH was adjusted to 5.2 with citric acid again. The mixture was stirred in a 30℃ water bath for 2 hours. After the reaction was completed, the temperature was raised to 65℃ and kept for 20 minutes to inactivate the enzyme. The mixture was homogenized at 5000 rpm for 5 minutes to obtain a chickpea flour mixture. S3. Oil phase preparation: 15g of lecithin was added to 175g of 70wt.% ethanol solution and stirred at 400 rpm for 20min until dissolved to obtain egg yolk powder ethanol solution. 0.05g of vitamin A, 0.03g of vitamin D3, and 0.25g of vitamin E were added to 99.67g of flaxseed oil and stirred until dissolved to obtain flaxseed oil mixture. This mixture was then mixed with the lecithin ethanol solution and sonicated at 300W for 20min to obtain oil phase mixture. S4. Emulsion preparation: Slowly add the above 300g oil phase mixture to 150g chickpea flour mixture, homogenize to form a primary emulsion, then add 150g of lactoferrin and bee pollen mixture solution, wherein the lactoferrin content is 2wt.% and the bee pollen content is 1wt.%, adjust the pH to 6.4, homogenize again at 9000 rpm for 15min, and then spray dry to obtain the functional ingredient; S5. Basic raw material pretreatment: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g calcium carbonate, 0.5g zinc gluconate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 96.75g water to prepare excipients; S6. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of auxiliary materials and 100g of functional ingredients are added to a chopper in batches and mixed. The mixture is then filled and sterilized at 121℃ for 30 minutes to obtain a nutritional supplement for pregnant pets. Comparative Example 6
[0034] The difference between this comparative example and Example 1 is that chickpeas are replaced with wheat that does not contain soy isoflavones, as detailed below: S1. Wheat enzymatic hydrolysis: 100g of wheat flour that has passed through an 80-mesh sieve is mixed with 300g of water to form a slurry. The pH is adjusted to 5.2 with citric acid, and protease is added to make the protease activity reach 3000U / g. The mixture is stirred at 200 rpm for 1.5h at a constant temperature of 45℃ to obtain the enzymatically hydrolyzed slurry. S2. GABA enrichment: GAD enzyme was added to the enzymatically hydrolyzed slurry to make the protease activity reach 3000U / g. After stirring evenly, 3g of calcium chloride was dissolved and added slowly in 3 batches, with an interval of 15min between each batch. The reaction was carried out in a 30℃ water bath for 2h with stirring. After the reaction was completed, the temperature was raised to 65℃ and kept at 20min to inactivate the enzyme. The mixture was homogenized at 5000rpm for 5min to obtain a wheat flour mixture. S3. Oil phase preparation: 35g of egg yolk powder was added to 175g of 70wt.% ethanol solution and stirred at 400 rpm for 20min until dissolved to obtain egg yolk powder ethanol solution. 0.05g of vitamin A, 0.03g of vitamin D3, and 0.25g of vitamin E were added to 99.67g of flaxseed oil and stirred until dissolved to obtain flaxseed oil mixture. This mixture was then mixed with the egg yolk powder ethanol solution and sonicated at 300W for 20min to obtain oil phase mixture. S4. Emulsion preparation: Slowly add the above 300g oil phase mixture to 150g wheat flour mixture, homogenize to form a primary emulsion, then add 150g of lactoferrin and bee pollen mixture solution, wherein the lactoferrin content is 2wt.% and the bee pollen content is 1wt.%, adjust the pH to 6.4, homogenize again at 9000 rpm for 15min, and then spray dry to obtain the functional ingredient; S5. Basic raw material pretreatment: Thaw chicken breast and tuna, steam chicken breast for 25 minutes, grind and sieve angelica, ginseng and astragalus, heat and melt chicken oil and fish oil and filter, dissolve 1g taurine, 0.5g ferrous fumarate, 0.5g vitamin C, 0.15g L-carnitine and 0.1g folic acid in 97.75 water to prepare excipients; S6. Mixing and Molding: The raw materials include 50wt.% chicken breast, 15wt.% tuna, 5wt.% chicken liver, 3wt.% chicken fat, 2wt.% fish oil, 0.5wt.% angelica, 0.5wt.% ginseng, 0.5wt.% astragalus, and the remainder is water. 400g of raw materials, 75g of auxiliary materials and 100g of functional ingredients are added to a chopper in batches and mixed. The mixture is then filled and sterilized at 121℃ for 30 minutes to obtain a nutritional supplement for pregnant pets. Indicator Test
[0035] Palatability study During the experiment, the weight, body size, and living environment of all cats remained consistent before and after the experiment. They were divided into groups of 18 cats each, and food was provided for free access daily. They were fed cat food supplemented with nutritional supplements in the same environment for 28 days, and the average daily food intake of each cat was recorded. The group without supplements served as a control. The preferred choice rate and consumption rate of the experimental cat food were recorded.
[0036] The experimental results are shown in Table 1. The average daily intake of the examples was over 195g. The first-choice rate (83-88%) and intake rate (89-92%) of the example groups were significantly higher than those of the control group. Among them, Example 2 had the highest intake rate and the best palatability. Control group 4 had the worst palatability, indicating that without packaging, the product may have an off-flavor that affects intake. Control group 2 had a high intake rate but a decreased intake, indicating a decrease in total food intake. This suggests that the enriched GABA can alleviate anorexia during pregnancy or improve mood and increase food intake.
[0037] Table 1. Results of palatability test
[0038] Blood samples were taken from the experimental cats to conduct blood tests. Blood test results showed that progesterone levels were increased in all experimental groups compared to the control group, with the highest progesterone levels observed in the serum of the group fed Example 1. This indicates that the present invention improves progesterone levels, and the preparation under the refined process of the present invention yields the best results. The control group had low hemoglobin and serum calcium levels, indicating a need for calcium and iron supplementation. Supplementation improved hemoglobin and serum calcium levels in the experimental groups, particularly in Example 1, while Comparative Example 5 showed no significant change, suggesting that simultaneous addition affected absorption. Similarly, the serum EPA / DHA ratio in Example 1 was higher than in Comparative Examples 3 and 4, indicating that flaxseed oil mixed in emulsion form is more easily absorbed, improving bioavailability.
[0039] Table 2. Relevant blood test results of pregnant cats in the feeding experiment.
[0040] Follow-up monitoring of dogs fed nutritional supplements Changes in progesterone levels in dogs at different gestation stages were recorded 7 days after consuming the supplement.
[0041] The results are shown in Table 3. In the early stages, progesterone levels increased significantly after supplementation, particularly in Example 1 where the increase was 8 ng / mL. In the mid-pregnancy stage, progesterone levels in most samples increased slightly or remained stable, with smaller increases. Late-pregnancy supplementation resulted in the smallest changes in progesterone levels; some samples showed a slight increase, while others remained stable or decreased. This may be related to the decrease in progesterone levels in late pregnancy, which may explain why the increase in progesterone levels after supplementation was not significant, but there was also no significant decrease. Example 1 showed a much higher absolute increase and relative increase rate than all other control groups. Control group 2 in late pregnancy showed poor results, indicating that the stabilizing effect of GABA on mood in late pregnancy can also affect progesterone levels.
[0042] Table 3. Changes in progesterone levels in dogs at different stages of pregnancy.
[0043] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Any person skilled in the art can make many possible variations and modifications to the technical solutions of the present invention, or modify them into equivalent embodiments, without departing from the spirit and technical essence of the present invention. Therefore, any simple modifications, equivalent substitutions, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention, without departing from the content of the technical solutions of the present invention, shall still fall within the scope of protection of the present invention.
Claims
1. A method of preparing a pet nutritional supplement for use during pregnancy, characterized in that, The method comprises the following steps: S1. Chickpea enzymatic treatment: chickpea powder is sieved, water is added to prepare a slurry, the pH is adjusted with citric acid, a protease is added, and constant temperature stirring is performed to obtain an enzymatic slurry; S2. GABA enrichment: after the enzymatic slurry is uniformly stirred with GAD enzyme, calcium chloride is slowly added in batches, water bath stirring is performed, after the reaction is completed, the temperature is increased to inactivate the enzyme, and homogenization is performed to obtain a chickpea powder mixture; S3. Oil phase preparation: egg yolk powder is added to an ethanol solution, stirring is performed until the egg yolk powder is dissolved to obtain an egg yolk powder ethanol solution, fat-soluble vitamins are added to flaxseed oil to obtain a flaxseed oil mixture, the flaxseed oil mixture is mixed with the egg yolk powder ethanol solution, and ultrasonic treatment is performed to obtain an oil phase mixture; S4. Emulsion preparation: the oil phase mixture is slowly added to the chickpea powder mixture, homogenization is performed to form a primary emulsion, a lactoferrin and bee pollen mixed solution is added, the pH is adjusted, and homogenization is performed again, and then spray drying is performed to obtain a functional ingredient; S5. Base raw material pretreatment: chicken breast and tuna are thawed, the chicken breast is steamed, angelica, ginseng and astragalus are crushed and sieved, chicken oil and fish oil are heated, melted, filtered, taurine, ferrous fumarate, vitamin C, L-carnitine and folic acid are dissolved and compounded to form an auxiliary material; S6. Mixing and molding: the raw materials, auxiliary materials and functional ingredients are put into a chopper in batches, chopping is performed, the mixture is filled and sterilized to obtain a pregnant pet nutritional supplement.
2. The method for preparing a nutritional supplement for pregnant pets according to claim 1, characterized in that: In the step S1, the mass ratio of chickpea powder to water is (1-2):(6-8); the pH is adjusted to 5.2-5.5; the amount of protease added in the slurry is 3000-5000 U / g; the constant temperature is 45-55°C; the stirring speed is 200-500 rpm, and the time is 1-2 h.
3. The method for preparing a nutritional supplement for pregnant pets according to claim 1, characterized in that: In the step S2, the amount of GAD enzyme added in the enzymatic slurry is 1000-3000 U / g; the batches are divided into 3 batches, and the interval between each batch is 15-25 min; the water bath stirring temperature is 30-50°C, the time is 1.5-2.5 h; the amount of calcium chloride added is 3-5 wt.% of the mass of chickpea powder; the temperature for inactivating the enzyme is 65-85°C, the time is 20-30 min; the homogenization speed is 2000-5000 rpm, and the time is 5-10 min.
4. The method for preparing a nutritional supplement for pregnant pets according to claim 1, characterized in that: In the step S3, the concentration of the ethanol solution is 70-80 wt.%; the mass ratio of egg yolk powder to ethanol solution is 1:(5-9); the stirring speed is 400-600 rpm, and the time is 20-40 min; the fat-soluble vitamins are vitamin A, vitamin D3 and vitamin E; the amount of fat-soluble vitamins added is 0.1-0.3 wt.% of the mass of flaxseed oil; the mass ratio of the flaxseed oil mixture to the egg yolk powder ethanol solution is 1:(1-3); the ultrasonic power is 200-500 W, and the ultrasonic time is 10-20 min.
5. A method of preparing a pet nutritional supplement for gestation according to claim 4, characterized in that: The mass ratio of vitamin A, vitamin D3 and vitamin E is (5-6):(2-3):(25-30).
6. The method for preparing a nutritional supplement for pregnant pets according to claim 1, characterized in that: The mass ratio of the oil phase mixture, chickpea powder mixture, lactoferrin and bee pollen mixed solution in step S4 is (1-2):1:1; the rotation speed of homogenization is 8000-10000 rpm, and the time is 10-20 min; the content of lactoferrin in the lactoferrin and bee pollen mixed solution is 2-3 wt.%, and the content of bee pollen is 1-2 wt.%; the pH is adjusted to 6.4-6.6; the rotation speed of homogenization is 9000-12000 rpm, and the time is 10-20 min.
7. The method for preparing a nutritional supplement for pregnant pets according to claim 1, characterized in that: The cooking time in step S5 is 25-30 min; the content of taurine in the auxiliary material is 1 wt.%, the content of ferrous fumarate is 0.5 wt.%, the content of vitamin C is 0.5 wt.%, the content of L-carnitine is 0.15 wt.%, and the content of folic acid is 0.1 wt.%.
8. The method for preparing a nutritional supplement for pregnant pets according to claim 1, characterized in that: The raw materials in step S6 include chicken breast 40-50 wt.%, tuna 15-20 wt.%, chicken liver 5-10 wt.%, chicken oil 2-5 wt.%, fish oil 1-2 wt.%, angelica 0.3-0.5 wt.%, ginseng 0.5-0.7 wt.%, and astragalus 0.4-0.6 wt.%, with the balance being water; the mass ratio of the raw materials, auxiliary materials and functional ingredients is (80-90):(15-20):(10-20); the sterilization temperature is 121℃, and the time is 30-50 min.
9. The pet nutritional supplement during pregnancy prepared by the preparation method according to any one of claims 1-8.