Lung cancer cell culture tank with ozone sterilization function

Abstract

A lung cancer cell culture tank with an ozone sterilization function belongs to the field of biotechnology and cell culture equipment, and is characterized in that a sealing door (2) is arranged at the front part of a tank body (1), a 8W UVC ultraviolet lamp tube (3) is mounted at the top in the tank, and a nano titanium dioxide photocatalyst coating is sprayed on the inner wall of the tank body to form an ultraviolet sterilization and ozone management module; the tank bottom wall (18) is provided with a semiconductor chilling plate (5) and a temperature sensor (6); an air filter (7) with a 0.22 mu m hydrophobic filter membrane is arranged at the upper part of the rear wall (11) of the tank body; the semiconductor chilling plate (5), the temperature sensor (6) and the air filter (7) jointly form a cell culture environment maintaining system; a glove hole (4) is formed in the center of each tank body side wall (12) and is connected with an operation glove (17) through a telescopic sleeve (16); the control panel (8) is located on the front face of the tank body. The invention has the advantages of simple manufacture, convenient use, low cost and obvious effect.

Description

I. Technical Field

[0001] This invention relates to an integrated culture vessel capable of generating ozone for sterilization, enabling aseptic operation and culture of lung cancer cells in a non-sterile laboratory environment, and belongs to the field of biotechnology and cell culture equipment. II. Background Technology

[0002] Lung cancer cell culture is a crucial tool for cancer research, drug screening, and personalized medicine. Currently, conventional cell culture procedures heavily rely on large, specialized equipment, such as laminar flow hoods (for aseptic operation) and CO2 incubators (to provide a constant temperature, humidity, and gas environment). However, this approach has significant drawbacks: strong equipment dependence (it must be performed in a standard cell laboratory and cannot be used in common environments such as wards or field sampling sites); cumbersome operation (samples need to be transferred between different devices, increasing the risk of contamination); high cost (laminar flow hoods and CO2 incubators are expensive and have high maintenance costs); and space limitations (restricting cell culture research in resource-constrained environments). Therefore, there is an urgent need in this field for a miniaturized, integrated device capable of independently completing the entire sterilization and culture process. III. Summary of the Invention

[0003] This invention aims to overcome the shortcomings of existing technologies and provide a compact and easy-to-operate lung cancer cell culture vessel. Its core objective is to autonomously create and maintain a sterile environment within the vessel through a built-in ultraviolet-ozone sterilization system. This allows users to simply place samples into the vessel and perform aseptic operations and cell culture in ordinary environments (such as a regular desktop), without relying on any external sterile equipment.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] A lung cancer cell culture vessel with ozone sterilization function includes: a vessel body (1) which is a sealed, opaque (or partially transparent) container, the inner cavity of which constitutes a cell culture and operation space. A sealed door (2) is provided on the vessel body (1) for taking out and putting in samples. An 8W UVC ultraviolet lamp tube (3) is installed on the top or side wall of the vessel to emit ultraviolet light (preferably UVC band, such as 254nm). After the ultraviolet light irradiates the air (oxygen) in the vessel, ozone (O3) will be generated naturally. The inner wall of the vessel is sprayed with a nano-titanium dioxide photocatalytic coating, which can accelerate the decomposition of residual ozone into harmless oxygen in the later stage of sterilization or culture under ultraviolet photocatalysis. Alternatively, a simple delayed ventilation program can be set up to discharge ozone through a small fan and filter valve. The ultraviolet lamp tube (3) and the nano-titanium dioxide photocatalytic coating form an ultraviolet sterilization and ozone management module, which is connected to the power supply through a control circuit. A semiconductor cooling chip (5) is integrated at the bottom of the tank, and its hot end is connected to the external heat sink and fan. A DS18B20 temperature sensor (6) is installed inside the tank and connected to the control circuit with an STM32 microcontroller as the core. The temperature is precisely controlled at 37±0.2℃ through the PID algorithm. An air filter (7) with a 0.22μm hydrophobic filter membrane is provided at the upper rear of the tank. The air filter (7) is a vent with a sterile filter on the tank, which allows gas exchange and prevents external microorganisms from entering. An interface can be reserved at the air filter (7) for injecting a specific proportion of CO2 mixed gas when needed, or using commercial CO2 buffered culture medium (such as HEPES) to maintain pH stability. A small water tray (10) is set inside the tank to maintain a high humidity environment by evaporating the water of the culture medium itself and prevent excessive evaporation of the culture medium. The semiconductor cooling chip (5), temperature sensor (6), water tray (10) and air filter (7) together constitute the cell culture environment maintenance system. The tank has a pair of sealed glove holes (4) on both sides, which can be inserted into the operating arm and connected to a rubber glove to form an operating interface for users to perform operations such as pipetting and passage inside the tank. The control panel (8) is located on the front of the tank and is the "brain" and "human-computer interaction center" of the entire culture tank. Its core design is intuitive, reliable and safe.

[0006] The beneficial effects of this invention are as follows:

[0007] 1. High integration and independence: It integrates sterilization, operation and culture functions into one, eliminating the dependence on laminar flow hoods and carbon dioxide incubators, and truly achieving "one tank does it all".

[0008] 2. Excellent sterilization effect: Ultraviolet light and the ozone it produces are both powerful sterilizing agents that can kill bacteria, fungi, viruses and their spores, sterilizing without dead corners and significantly reducing the contamination rate.

[0009] 3. Simple and safe operation: Users only come into contact with the container before and after sterilization. The operation is carried out inside the sealed container, which protects both the sample and the operator.

[0010] 4. Wide range of applications: It is particularly suitable for environments that do not have high-level sterile facilities, such as hospital bedsides, field investigations, and primary laboratories, making it possible to provide timely diagnosis and research for lung cancer.

[0011] 5. High cost-effectiveness: The equipment manufacturing cost is much lower than that of traditional large-scale equipment combinations, which is conducive to promotion and popularization. IV. Description of the attached drawings

[0012] Figure 1 : A three-dimensional schematic diagram of the overall structure of the culture tank of the present invention.

[0013] Figure 2 : Schematic diagram of the sealing door structure of the culture tank of the present invention.

[0014] Figure 3 : Schematic diagram of the sealing structure between the sealing door and the sealing door frame of the culture tank of the present invention.

[0015] Figure 4 : A schematic diagram of the working process of the culture tank of the present invention.

[0016] In the diagram: 1. Tank body, 2. Sealed door, 3. UV lamp, 4. Glove hole, 5. Semiconductor cooling chip, 6. Temperature sensor, 7. Air filter, 8. Control panel, 9. Electrical and functional interface, 10. Water tray, 11. Rear wall of tank body, 12. Side wall of tank body, 13. Power cord, 14. Tank handle, 15. Shelf, 16. Telescopic sleeve, 17. Operating gloves, 18. Bottom wall of tank, 19. Inner cavity of tank, 20. Sealed door frame, 21. Sealed door frame side groove, 22. Sealing magnetic strip, 23. Sealed door frame cavity, 24. Inner and outer sealing strips, 25. Iron-edged glass door, 26. Door handle, 27. Door iron edge, 28. Door hinge. V. Detailed Implementation Methods

[0017] Example 1

[0018] refer to Figure 1 and Figure 2The present invention discloses a lung cancer cell culture vessel with ozone sterilization function, which consists of a vessel body (1), a sealing door (2), an ultraviolet lamp (3), a glove hole (4), a semiconductor cooling chip (5), a temperature sensor (6), an air filter (7), a control panel (8), an electrical and functional interface (9), a water tray (10), a rear wall of the vessel body (11), a side wall of the vessel body (12), a power cord (13), a handle of the vessel body (14), a shelf (15), a telescopic sleeve (16), operating gloves (17), a bottom wall of the vessel body (18), an inner cavity of the vessel body (19), a door frame of the sealing door (20), a side groove of the door frame of the sealing door (21), a sealing magnetic strip (22), a cavity surrounding the door frame of the sealing door (23), inner and outer sealing strips (24), a glass door with iron edge (25), a door handle (26), a door iron edge (27), and a door hinge (28). The vessel body (1) is composed of a stainless steel inner liner and an external insulation material, with dimensions of approximately 40cm long x 30cm wide x 30cm high. A transparent sealing door (2) is provided at the front of the tank (1), which can be sealed by magnetic force; an 8W UVC ultraviolet lamp tube (3) is installed at the top inside the tank, and the inner wall of the tank is sprayed with a nano titanium dioxide photocatalytic coating to form an ultraviolet sterilization and ozone management module. A semiconductor cooling chip (5) is integrated on the bottom wall (18), and its hot end is connected to the external heat sink and fan; a DS18B20 temperature sensor (6) is installed in the bottom wall (18), which is connected to the control circuit with STM32 microcontroller as the core, and the temperature is precisely controlled at 37±0.2℃ through PID algorithm; an air filter (7) with a 0.22μm hydrophobic filter membrane is provided on the upper part of the rear wall (11) of the tank; the semiconductor cooling chip (5), the temperature sensor (6) and the air filter (7) together constitute the cell culture environment maintenance system. Each tank sidewall (12) has a glove hole (4) in the center, through which an operating glove (17) is connected via a telescopic sleeve (16) for internal operation. The control panel (8) is located on the front of the tank and is the "brain" and "human-machine interaction center" of the entire culture tank. Its core design principles are intuitiveness, reliability, and safety.

[0019] (I) Detailed structural components and functions of the tank (1)

[0020] The container (1) is a sealed, multifunctional container that integrates a sterile operating space and cell culture function. Its core design principle is to achieve absolute isolation from the external environment within a minimal space through precise mechanical and material design, while simultaneously providing stable and controllable cell culture conditions. Detailed structural components and functions of the container (1) are described below:

[0021] 1. Structure and materials of the main tank: (1) Outer layer: Made of robust engineering plastics (such as ABS) or metals (such as anodized aluminum) to provide structural support and protection. The outer layer can be designed to be basically opaque to prevent ambient light from interfering with cell growth and to avoid ultraviolet leakage. (2) Inner layer and inner cavity: The inner wall is made of highly biocompatible, corrosion-resistant, easy-to-clean and disinfect materials, such as 316L stainless steel or medical-grade polycarbonate. The inner wall surface should be mirror polished to reduce dead corners and facilitate cleaning and sterilization. The inner cavity is the cell culture and operation space, where all aseptic operations (inoculation, passage, medium change, observation) are completed. (3) Insulation layer: Vacuum insulation board or polyurethane foam insulation material is filled between the outer and inner layers. This is a crucial part, which can effectively reduce the heat exchange between the internal temperature and the external environment, significantly reduce the energy consumption of the temperature control system (semiconductor cooling chip), and maintain a high degree of internal temperature stability. The main tank is rectangular. The cuboid shape has high space utilization and is convenient for placing standard petri dishes / bottles.

[0022] 2. Sealed door (2): This is a key component to ensure airtightness. The design must take into account airtightness, visibility and convenience.

[0023] (1) Structure of the sealed door (2): The magnetic door is composed of a sealed door frame (20), a sealed door frame side groove (21), a sealing magnetic strip (22), a sealed door frame cavity (23), inner and outer sealing strips (24), an iron-edged glass door (25), a door handle (26), a door iron edge (27), and a door hinge (28). The sealed door frame (20) is made of stainless steel or aluminum alloy and is a rectangular frame with an inwardly recessed groove, i.e., the cross-section is L-shaped. The sealed door frame (20) includes two parts: the frame and the support frame. The length of the frame is 1-2 cm and the width is 0.5-1 cm. After welding multiple points between the outer surface of the frame and the front wall of the tank (1) in the length direction, the gaps are filled with glass glue to seal and adhere them together. The support frame is the part that extends into the cavity (23) of the sealed door frame, with a length of 2-3 cm and a width of 0.5-1 cm; the door frame groove (21) is the groove formed by the frame of the sealed door frame (20) and the support frame on the outer part of the sealed door frame (20) to connect the iron-edged glass door (25) and the sealing magnetic strip (22), so that a beautiful embedded sealing connection is formed between the sealed door frame (20) and the iron-edged glass door (25). The depth of the door frame groove (21) is 0.5-1 cm; the sealing magnetic strip (22) is a long strip permanent magnet fixed in the center of the outer surface of the support frame of the sealed door frame (20). For sealing, a silicone material with an isosceles right triangle cross-section is set on the outside of the magnet. The outer casing is attached to the outer surface of the door frame (20) support frame by adhesive. Once the door is closed, a seal is achieved between the door frame (20) and the iron-edged glass door (25). The door frame cavity (23) is the cavity enclosed by the door frame (20) support frame, which is the channel for placing or removing foreign objects into or from the inner cavity (19) of the can. The iron-edged glass door (25) is a door with iron edges around the perimeter and transparent glass in the center. It is 0.5-1 cm thick and is movably connected to the door frame (20) by door hinges (28) so that the iron-edged glass door (25) can be opened or closed. The iron edge (27) is the iron edge around the iron-edged glass door (25). The iron edges (27) of the top, bottom and outer edges of the edge glass door (25) have a U-shaped cross-section. The U-shaped inner cavity is embedded in the glass edge of the iron edge glass door (25). On the inner edge of the iron edge glass door (25), the iron edge (27) has a μ-shaped cross-section. The μ-shaped inner cavity is embedded in the glass edge of the iron edge glass door (25). The μ-shaped protruding wings of the two iron edge glass doors (25) are close together. The outer surface is used to adhere and seal with the middle and outer sealing strips (24), thereby sealing the gap between the two iron edge glass doors (25) that are closed and close together. A groove with a depth of 0.5-1 cm and a width of 0.5-1 cm is formed on the outside of the μ-shaped protruding wings so that the embedding strip of the middle and outer sealing strips (24) can be inserted.The inner and outer sealing strips (24) are made of rubber or plastic, with a T-shaped cross-section. They consist of a handle strip and an insert strip. The length of the inner and outer sealing strips (24) is the same as the length of the iron-edged glass door (25). The handle strip is 2-3 cm wide and 2-3 cm thick, making it easy for the operator to hold the inner and outer sealing strips (24) and remove or insert them from the groove between the two iron-edged glass doors (25). The insert strip is 0.5-1 cm thick and 0.5-1 cm wide. One outer surface is connected to the inner surface of the handle strip to form a whole and is located in the center of the inner surface of the handle strip. The other outer surface is connected to the inner surface of the handle strip to form a whole. Two sealing magnetic strips (22) are provided on each surface. After the middle and outer sealing strips (24) are embedded in the groove between the two iron-edged glass doors (25), the sealing magnetic strips (22) can be attracted together with the μ-shaped protruding wings by magnetic force and seal the gap between the two iron-edged glass doors (25). When it is necessary to open the iron-edged glass door (25), first remove the middle and outer sealing strips (24) from the groove, and then pull the door handle (26) to open the iron-edged glass door (25). When it is necessary to close, hold the door handle (26), push the two iron-edged glass doors (25) together, and then insert the middle and outer sealing strips (24). The door handle (26) is made of stainless steel or aluminum alloy and has the same structure as known door handles. It is set on the inner edge of the iron-edged glass door (25). The connector is equipped with a sealing gasket. After tightening with a screwdriver, the inside and outside are sealed and no gaps will appear. The door hinge (28) has the same structure and function as known door hinges. It is made of stainless steel and is connected between the iron-edged glass door (25) and the sealed door frame (20) to form a movable connection between the iron-edged glass door (25) and the sealed door frame (20).

[0024] The material of the sealed door (2): The main body is metal or engineering plastic, forming the iron edge (27) of the iron-edged glass door (25). A transparent observation window is integrated in the center of the iron-edged glass door (25). The observation window should adopt a double-layer structure: Inner layer: UV-resistant quartz glass or special UV-transmitting glass, allowing ultraviolet rays to pass through for sterilization. Outer layer: Ordinary tempered glass, which serves a protective function. Between the two layers, a metal micro-mesh can be sandwiched as a physical barrier against ultraviolet leakage, ensuring that even if the inner glass breaks, ultraviolet rays will not leak out in large quantities, protecting the operator's safety.

[0025] 3. Operating interface: It is the key gateway to realizing the concept of "performing aseptic operations in ordinary environments". Its design directly determines the ease of operation, comfort and, most importantly, the reliability of the system's sealing.

[0026] Design Goals and Core Considerations for the Operating Interface: Primary Goal: To maintain absolute airtightness of the tank while allowing operators to access it with both arms, preventing the entry of external contaminants and leakage of internal ozone / UV radiation. Core Considerations: ① Sealing Reliability: Absolutely foolproof. ② Operational Flexibility and Tactile Feedback: Allowing for delicate cellular manipulations (such as pipetting and scraping) while maintaining hand dexterity and tactile feedback. ③ Material Durability: Materials must be resistant to ozone and UV aging and possess sufficient mechanical strength. ④ Ergonomics: Reducing operator fatigue. ⑤ Cost and Complexity: Simplifying as much as possible while meeting requirements.

[0027] Fixed glove port system: This is the most reliable solution, offering the experience closest to a traditional biosafety cabinet. The structural components and functions of the fixed glove port system are as follows:

[0028] (1) Tank Flange: Structure and Material of Tank Flange: Two short pipes welded or bolted to the side wall of the tank, made of the same material as the inner wall of the tank (e.g., 316L stainless steel). Their end faces are precision machined to ensure flatness. Function of Tank Flange: Serving as the rigid foundation and main load-bearing structure of the entire glove interface.

[0029] (2) Main sealing ring: Structure and material of the main sealing ring: an O-ring or rectangular flat gasket made of ozone-resistant silicone rubber. The function of the main sealing ring is to be placed in the sealing groove on the end face of the tank flange, and it is the first and most important barrier to prevent the exchange of internal and external gases.

[0030] (3) Glove ring: Structure and material of the glove ring: A robust ring-shaped component, usually made of stainless steel or ozone-resistant engineering plastics (such as PVDF). The function of the glove ring is to press the wrist of the glove against the main sealing ring.

[0031] (4) Quick-opening clamps: Structure and material of quick-opening clamps: A single-ear or double-ear hinged clamp, usually made of stainless steel. Function of quick-opening clamps: This is the core of achieving quick assembly and disassembly. By rotating the handle on the clamp, a huge locking force can be generated, pressing the glove ring evenly against the tank flange, causing the main sealing ring to deform and achieve absolute sealing. During disassembly, simply loosen the clamp to change the operating gloves (17).

[0032] (5) Operating glove (17): The material is ozone-resistant butyl rubber, which has excellent airtightness, chemical resistance and puncture resistance. The functions of the operating glove (17) are: First, to operate the actuator: the operator's hands are inserted into it to directly perform all delicate operations. Second, to act as a secondary sealing barrier: the glove itself is integrated with the container through clamps, forming part of the sealing system. Third, to transmit tactile feedback: the thin and tough butyl rubber can provide good tactile feedback while ensuring safety, making it convenient for the operator to feel the force of the pipette button, etc. The telescopic sleeve (16) is the connecting part between the operating glove (17) and the glove hole (4). It consists of an inner and outer structure. The inner layer is made of ozone-resistant butyl rubber and the outer layer is made of cotton fiber cloth. One end is fixed to the periphery of the glove hole (4) and the other end is fixed to the glove ring, so as to ensure that there is no seam between the operating glove (17), the telescopic sleeve (16) and the glove hole (4), thus achieving the purpose of sealing.

[0033] Workflow of the fixed glove hole system: The operator inserts both hands into the gloves. All operations are performed within the sealed container. After use, the hands are removed. To change gloves, loosen the clamps to remove the old gloves, put on the new gloves, and then tighten the clamps again. If the operator is prone to sweaty hands, they can first wear a pair of thin fiber gloves with a thickness of 0.1 mm before inserting them into the operating gloves (17). This ensures that there is a gap for sweat to escape and also makes it easier to insert both hands into the gloves.

[0034] Through this comprehensive design with fixed and replaceable gloves, the operating interface of the culture vessel of this invention achieves an optimal balance in terms of sealing, functionality, safety and user experience, supporting its core positioning as an "integrated sterile cell workstation".

[0035] 4. Electrical and functional interfaces: These interfaces need to pass through the tank wall and must be strictly sealed.

[0036] (1) Cable connectors: Cables that supply power and transmit signals to the UV lamp (3), temperature sensor (6), and thermoelectric cooler (5) need to pass through the tank via waterproof aviation plugs or glands. These connectors have built-in rubber seals to ensure airtightness at the cable passage points.

[0037] (2) Ventilation port: One or more ventilation ports with sterile air filters (7) are installed on the tank. The air filter (7) uses a hydrophobic 0.22-micron polyethersulfone filter element, which allows slow exchange of air inside and outside the tank to balance the pressure, while absolutely blocking the entry of microorganisms such as bacteria and fungi. A quick-connect interface with a sealing cap is provided for connecting a gas cylinder when needed to inject a specific proportion of CO2 gas into the tank.

[0038] (3) Sensor interface: The probes of the temperature and humidity sensors are placed inside the tank, and their cables are also led out through the above-mentioned sealed connectors.

[0039] 5. Internal accessories and layout: The inner cavity (19) is the inner cavity of a lung cancer cell culture tank with ozone sterilization function. The inner cavity (19) is equipped with a shelf (15), a water tray (10) and a UVC ultraviolet lamp tube (3). The shelf (15) is a guide rail or slot set on the upper half of the inner wall of the tank (1). After inserting a stainless steel or plastic shelf, the shelf is formed. The shelf height is adjustable and it is used to place culture dishes, cell culture bottles, reagent tubes, etc. The water tray (10) is a shallow water tray placed on the lower inner surface of the tank. The water tray (10) is rectangular, 10 cm long, 5 cm wide, and 1 cm thick. A circular hole with a diameter of 0.8 cm is set in the center of the bottom of the water tray (10) for adding water and for evaporating water in the tray to keep it moist. The outer wall of the water tray (10) is made of plastic or glass with a thickness of 1-2 mm. The inner cavity of the water tray (10) is filled with porous wet sponge to store water but to ensure that the water does not overflow as much as possible. The high humidity environment (>95%) in the tank is maintained by the natural evaporation of water in the water tray (10) to prevent excessive evaporation of the culture medium. The ultraviolet lamp tube (3) is usually fixed to the top of the inner cavity to ensure that there are no dead angles for irradiation. The inner wall of the tank (1) (especially the back and the sides that are not frequently touched) can be coated with a nano titanium dioxide photocatalytic coating, which can catalytically decompose residual organic matter and ozone under ultraviolet light irradiation.

[0040] 6. Workflow and structure of the container (1): ① Preparation and placement: Open the sealed door (2), place the required items into the inner cavity (19) of the container, and place them on the shelf (15). ② Sealing and sterilization: Close and lock the sealed door. Turn on the ultraviolet lamp (3), and ultraviolet light shines through the quartz glass observation window to irradiate the entire mirror-polished inner cavity, generating ozone for sterilization. All sealed interfaces ensure that ozone and ultraviolet light do not leak out. ③ Aseptic operation: The operator inserts their hands into the inner cavity (19) of the container through the glove holes (4) to process lung cancer cell samples in an absolutely sterile environment. ④ Stable culture: After the operation is completed, the temperature control system maintains the internal temperature at 37°C efficiently through the insulation of the heat insulation layer. The air filter (7) performs slow gas exchange, and the water tray (10) maintains humidity. Cells grow in this stable environment. ⑤ Retrieval: After the culture is completed, a brief sterilization can be performed again, and then the sealed door (2) can be opened to take out the sample.

[0041] Through the detailed structural design described above, the culture vessel miniaturizes and integrates a complex cell laboratory environment into a robust, sealed, and intelligent "box," achieving the invention's objective of "performing sterile cell culture in ordinary environments."

[0042] (II) Detailed Structure and Functions of the Ultraviolet Sterilization and Ozone Management Module

[0043] The core function of the ultraviolet sterilization module is to emit ultraviolet light of a specific wavelength, serving as both a sterilization source and an ozone generator for the entire system.

[0044] 1. The core component of the ultraviolet sterilization module: the UVC ultraviolet lamp tube (3) that provides the ultraviolet light source.

[0045] (1) Selection of ultraviolet light source type:

[0046] Preferred option: Low-pressure mercury lamp. This is the most classic and economical choice. Approximately 85% of the energy in its emitted ultraviolet light is concentrated in the 254nm UVC band, which is precisely the peak wavelength absorbed by DNA, resulting in extremely high sterilization efficiency. Simultaneously, it also emits 185nm ultraviolet light, which is crucial for the photolysis of oxygen to produce ozone.

[0047] Alternative: UVC-LED. This is an emerging technology that is smaller, more robust, has a longer lifespan, and allows for instant switching. However, it is currently more expensive, and the power of a single LED is limited, requiring an array of multiple LEDs to achieve sufficient radiation intensity.

[0048] (2) Installation Location and Structure: ① Installation Location: Fixed to the top of the inner wall of the tank. This is the optimal location because ultraviolet light irradiates from top to bottom, minimizing blind spots blocked by items and ensuring the most comprehensive sterilization coverage. ② Fixing Method: Fixed to the top wall using UV-resistant and ozone-resistant plastic or ceramic brackets. Both ends of the lamp tube are connected to a high-frequency ballast or drive power supply via ozone-resistant silicone cables. ③ Protection Design: A perforated metal protective cover (the mesh size must ensure that it does not affect ultraviolet light transmission) can be installed on the outside of the lamp tube to prevent accidental damage to the lamp tube when handling items.

[0049] 2. Electrical and Control Systems of the Ultraviolet Sterilization Module

[0050] (1) Power Supply and Drive: ① The module connects to the external control circuit via a sealed cable interface on the tank wall. ② For low-pressure mercury lamps, a matching electronic ballast is required to provide a stable starting and operating voltage. For UVC-LEDs, a constant current drive power supply is required. ③ Safety Interlock: This is a crucial safety feature. The control circuit must be linked to the door cover switch. When the sealed door is opened, the circuit will forcibly cut off the power supply to the UV lamp, absolutely preventing UV leakage from causing eye and skin damage to operators.

[0051] (2) Control Logic: ① Set a timer knob or digital controller on the control panel (8) to allow users to set the sterilization time (e.g., 10-60 minutes). ② After startup, the UV lamp works, and the "Sterilizing" indicator light (usually purple or red) on the control panel lights up to remind the user not to open the door. ③ After the timer expires, the UV lamp automatically turns off, possibly accompanied by an audible prompt.

[0052] 3. Ozone formation and elimination

[0053] It is necessary to utilize the strong oxidizing properties of ozone to enhance the sterilization effect, while ensuring that it is safely removed before operation.

[0054] (1) Ozone generation: ① Principle: When the 185nm short-wave ultraviolet light emitted by the ultraviolet lamp (3) irradiates the air inside the tank (mainly composed of O2 and N2), the photon energy breaks the chemical bonds of oxygen molecules (O2) to generate oxygen atoms (O). These oxygen atoms then combine with other oxygen molecules to form ozone (O3). ② Reaction formula: O2 + photon (185nm) → 2O; O + O2 → O3. ③ Synergistic sterilization: Ozone (O3) is a strong oxidant that can penetrate the cell wall of microorganisms, destroy their DNA, RNA and proteins, leading to cell death. Its gaseous properties allow it to diffuse throughout the entire tank space, effectively compensating for the shadow dead zones that may be caused by the straight-line propagation of ultraviolet light. The combination of ultraviolet light and ozone constitutes a three-dimensional, dead-angle-free, powerful sterilization network.

[0055] (2) Ozone elimination: To ensure operator safety (ozone is irritating to the human respiratory tract) and cell health (high concentrations of ozone are toxic to cells), the ozone concentration in the tank must be reduced to a safe level after the sterilization process is completed.

[0056] ① Scheme 1: Passive photocatalytic decomposition (preferred scheme, automated): Structure and principle: A layer of nano-titanium dioxide photocatalytic coating is applied to the entire inner wall of the tank (including the top, sides and bottom, but excluding the operation interface and observation window). When the ultraviolet lamp (3) is working, TiO2 will generate electron-hole pairs under the excitation of ultraviolet light. These holes and electrons can react with water vapor and oxygen in the air to generate hydroxyl radicals and superoxide anions with extremely strong oxidizing power. These active groups can not only assist in sterilization, but also catalytically decompose ozone (O3) into harmless oxygen (O2). Reaction formula: O3+OH→O2+HO2. Advantages: Automatic operation: Sterilization and decomposition processes are carried out simultaneously without additional control. No additional energy consumption: It utilizes the ultraviolet light energy of the sterilization stage. Continuous purification: During cell culture, even if trace amounts of organic matter are volatilized, the photocatalyst can continuously decompose them under light, keeping the air fresh.

[0057] Option 2: Active Delayed Ventilation Purification (Backup Option, Fast): Structure and Principle: A small, low-noise DC fan is installed on the tank, with its inlet leading into the tank and its outlet connected to an ozone decomposition filter (filled with catalysts such as MnO2 and activated carbon). Control Logic: After the user-defined UV sterilization program ends, the system automatically enters the "purification stage." At this time, the UV lamps are off, but the fan starts, forcibly blowing the ozone-rich air inside the tank through the decomposition filter, converting it into oxygen before it is discharged back into the tank or to the outside (if discharged to the outside, the outlet must have an anti-contamination filter membrane). Advantages: Fast removal speed: Active airflow can quickly reduce ozone concentration. Disadvantages: First, it adds a moving part (fan), which has the risk of failure and noise; second, it requires additional control and energy; third, if the exhaust is discharged to the outside, there is a potential risk of sealing and contamination.

[0058] ③ Option 3: Using Option 1 as the primary method, photocatalysis is employed for continuous and passive decomposition during and after sterilization. Option 2 can be retained as an optional function; when users urgently need to use the culture vessel quickly, they can manually activate the "rapid purification" mode, using a fan to accelerate the cleaning process.

[0059] (3) Ozone generation and elimination module workflow: ① Start-up: The user closes the tank door, sets the sterilization time, and starts the program. ② Sterilization: The ultraviolet lamp illuminates, emitting 254nm and 185nm ultraviolet light. The 254nm ultraviolet light directly destroys microbial DNA. The 185nm ultraviolet light generates ozone for gas-phase sterilization. Under ultraviolet light excitation, the photocatalyst begins to decompose organic matter and ozone. ③ Purification: (Main Mode): After sterilization, the system enters a static waiting period. At this time, the photocatalyst continues to slowly decompose the remaining ozone under the residual ultraviolet light and ambient light. Usually, after 30-60 minutes of static waiting, the ozone concentration will drop to a safe level. (Fast Mode): After sterilization, the system automatically starts the fan and runs for 10-15 minutes for forced purification. ④ Safe Operation: The "Safety Indicator" on the control panel changes from red to green, or the system emits a beep, indicating that the environment inside the tank is safe (ozone concentration is low, no ultraviolet light). Only then can the tank door be opened or operation performed.

[0060] The aforementioned ultraviolet sterilization and ozone management modules not only provide powerful sterilization capabilities but also ensure the ease of use and absolute safety of the entire device, supporting the core invention concept of "safe and worry-free sterile cell culture in ordinary environments".

[0061] (III) Cell Culture Environment Maintenance System

[0062] The cell culture environment maintenance system is crucial for ensuring the healthy proliferation of lung cancer cells within the container, requiring precise replication of the environmental conditions of a standard cell culture incubator. The overall goal of the cell culture environment maintenance system is to independently and stably maintain the following three core environmental parameters within a sealed container: 1. Temperature: kept constant at 37.0 ± 0.2℃. 2. Gas environment: providing oxygen and managing carbon dioxide to maintain the culture medium pH at 7.2-7.4. 3. Humidity: maintaining a relative humidity >95% to prevent culture medium evaporation.

[0063] 1. Temperature control system: It is an active, closed-loop, precise temperature control system.

[0064] The structure and components of the temperature control system are as follows:

[0065] (1) The core of the temperature control system for heating and cooling: a thermoelectric cooler (5). Selection and installation of the thermoelectric cooler (5): Select a Peltier element with appropriate power (e.g., 50-100W). The thermoelectric cooler (5) is sandwiched between the inner wall of the tank (usually the bottom or side wall) and the external heat sink, and good thermal contact is ensured by thermal grease. Working principle of the thermoelectric cooler (5): When direct current passes through it, one side absorbs heat (cold end) and the other side releases heat (hot end). By changing the direction of the current, heating or cooling can be achieved. This allows a single element to cope with excessively high or low ambient temperatures, achieving bidirectional precise temperature control.

[0066] (2) Thermal management subsystem of temperature control system: Cold end: The cold end of the thermoelectric cooler (5) is in close contact with the heat-conducting plate (such as an aluminum plate) on the inner wall of the tank, directly transferring cold / heat to the air inside the tank. Hot end: The hot end of the thermoelectric cooler (5) is connected to a large aluminum heat sink fin and equipped with a high-speed DC fan to force the heat to be blown to the external environment. This is the key to ensuring cooling efficiency. If the heat cannot be dissipated in time, the system will fail.

[0067] (3) The core of the temperature control system is the temperature sensor (6). The selection and installation of the temperature sensor (6) are as follows: a high-precision, digital temperature sensor, such as DS18B20 or PT1000, is used. The temperature sensor (6) is installed in an area with good air circulation inside the tank, but it cannot be in direct contact with the ultraviolet lamp or the petri dish, so as to measure the actual temperature of the culture environment.

[0068] (4) The brain of the temperature control system is the control circuit, and the core of the control circuit is a microcontroller, such as STM32 or Arduino. The algorithm of the control circuit is: running a PID control algorithm. The microcontroller continuously reads the value of the temperature sensor (6) and compares it with the set value (37℃). If the temperature is lower than 36.8℃, it outputs a positive current to heat the inside of the tank using the Peltier element. If the temperature is higher than 37.2℃, it outputs a reverse current to cool the inside of the tank using the Peltier element. The output of the control circuit: the magnitude and direction of the current output to the Peltier element are controlled by a MOSFET to achieve smooth power regulation and avoid drastic temperature fluctuations.

[0069] (5) Functional flow of the temperature control system: After the system is powered on, the temperature sensor (6) monitors the temperature T_current inside the tank in real time. The control circuit calculates the difference between T_set and T_current, and dynamically adjusts the direction and magnitude of the current applied to the semiconductor cooling chip (5) through PID calculation, so that it can accurately heat or cool, and finally stabilize the temperature at the set value of 37℃.

[0070] 2. Gas Environment Maintenance System: This system ensures the necessary conditions for cellular respiration and maintains a stable pH in the culture medium. The structure and components of the gas environment maintenance system are as follows:

[0071] (1) Basic gas exchange unit: Air filter (7). Structure of air filter (7): An interface is opened in the tank wall (usually on the top or side) to install a sterile ventilation filter. The core of the sterile ventilation filter is a hydrophobic, ozone-resistant 0.22μm PTFE or PES filter element. Air filter (7) allows slow diffusion and pressure equalization of air (O2) inside and outside the tank, but completely blocks the entry of bacteria, fungi and viruses. Function of air filter: To provide oxygen for lung cancer cells cultured primarily in response to ambient air (20% O2).

[0072] (2) CO2 environmental maintenance plan, there are two options: Plan A and Plan B. You can choose one of the two or combine them.

[0073] ① Option A: Buffered medium (recommended for the simplicity of this design). Principle: Directly use cell culture medium containing HEPES buffer. HEPES can very effectively stabilize the pH of the medium at 7.2-7.4 in atmospheric CO2 concentrations, without the need for additional CO2 gas control. This is the simplest option for achieving "normal environment culture".

[0074] Option B: Active CO2 Injection Interface (Optional Upgrade Function). Structure: A sealed quick connector is pre-installed on the tank, connecting to an external small CO2 cylinder or premixed gas bag (containing 5% CO2) via a hose. Control: Can be equipped with a simple solenoid valve and timer. Users can manually open the valve to inject gas for several minutes after inoculation, or perform more precise feedback control (requires a pH sensor, which is more expensive). Function: Provides standard culture conditions for special culture media or cell types with strict CO2 requirements.

[0075] (3) Functional flow of the gas environment maintenance system: In most cases, the gas environment requirements can be met by relying solely on air filters and HEPES buffered medium. When more precise culture is required, a short-term gas supply is provided through the CO2 injection interface to create a 5% CO2 environment.

[0076] 3. Humidity maintenance system: This is a passive but crucial system designed to prevent the culture medium from becoming concentrated due to evaporation during long-term culture, which could affect osmotic pressure and cell health.

[0077] The humidity maintenance system consists of a humidification source, which is a wide-mouthed, chemically inert Teflon or stainless steel water tray (10) placed at the bottom of the tank. A porous, wet sponge with a high specific surface area is fixed inside the water tray. During system operation, the temperature control system maintains the tank at 37°C. This temperature accelerates the evaporation of sterile water from the water tray or wet sponge. Within the sealed tank, water vapor cannot escape, causing the air inside the tank to quickly reach saturation, creating a high humidity environment of >95%. This high humidity environment significantly reduces the evaporation driving force on the surface of the culture medium, effectively preventing excessive evaporation of the culture medium.

[0078] The humidifier's functional flow is as follows: Users only need to add sufficient sterile distilled water to the water tray before first use or periodically. The system will automatically complete the humidification process during temperature control, requiring no additional control.

[0079] The three subsystems—temperature control, gas environment maintenance, and humidity maintenance—are not isolated but interconnected and work synergistically: ① Temperature is the core: The temperature control system provides a suitable temperature for the cells and also provides an evaporative heat source for the humidity system. ② Humidity and gas are the guarantees: The humidity system relies on temperature to operate, and a high humidity environment also reduces water loss from the culture medium during aeration. The gas system ensures pH and O2 stability based on a stable temperature. ③ Unified control: All electronic components (thermal cooling chips, fans, sensors) are managed by a central control circuit, allowing users to set and monitor the entire culture environment through a simple control panel.

[0080] Through this meticulously designed cell culture environment maintenance system, the culture vessel of this invention successfully miniaturizes and integrates the functions of a standard cell culture chamber into a sealed vessel, ensuring that lung cancer cells can thrive in a stable and suitable environment, completely eliminating the dependence on traditional large CO2 incubators.

[0081] (iv) Specific structural components and functions of the control panel (8)

[0082] Design concept of control panel (8): Clear functional zoning: Clearly divide the control and display areas of different functions to avoid misoperation. Clear interaction logic: The operation process is intuitive and the status feedback is clear at a glance. Safety first: Multiple safety protections are built in through physical and electronic means. Robust and durable: The panel material and components can adapt to the laboratory environment. Specific structure and functions of control panel (8): The control panel adopts embedded installation, flush with or slightly recessed with the surface of the tank to prevent accidental collision. Its structure can be divided into the following parts from top to bottom or according to functional blocks:

[0083] 1. Overall layout and casing of the control panel (8)

[0084] Panel material of control panel (8): industrial-grade ABS plastic or stainless steel, with anti-corrosion and scratch-resistant treatment on the surface. Installation method of control panel (8): embedded, fixed to the tank frame with screws on the back, with sealing rings at the seams to prevent the internal circuit from being affected by moisture or ozone. Main sections of control panel (8): information display area (top), temperature control area (middle left), ultraviolet sterilization control area (middle right), system power and status indicator area (bottom).

[0085] 2. Information display area

[0086] Core Component: LCD Display Screen. Type: A backlit LCD character display screen, approximately 4 lines x 20 characters in size. Functions: Real-time numerical display dynamically shows the following: Current temperature: 37.2℃, Set temperature: 37.0℃, UV time remaining: 00:00; Status indication displays the current system mode, such as [Ready], [Sterilizing], [Cooling], [Constant Temperature]; Alarm information provides clear alerts when abnormalities occur, such as [Alarm: Temperature sensor malfunction], [Please close the door].

[0087] 3. Temperature control zone

[0088] Core Component 1: Temperature Setting Encoder. Type: A rotary encoder with a press function. It has two functions: first, a rotation function, which, in temperature setting mode, increases or decreases the set temperature value (in 0.1℃ steps); second, a press function, used to confirm the temperature setting or enter temperature setting mode. Advantages: Faster and more precise value setting than simple "+" and "-" buttons.

[0089] Core Component 2: Temperature Control Status Indicator. Type: Two LED indicator lights, usually of different colors. Function: The green LED (constant temperature) is constantly lit to indicate that the temperature has reached the set value and is in a stable state; the red LED (heating / cooling) flashes to indicate that the system is actively heating or cooling to approach the set temperature.

[0090] 4. Ultraviolet sterilization control area

[0091] Core Component 1: Ultraviolet Sterilization Timer. Type: A multi-position mechanical knob switch or a digital "+" and "-" button. Function: The mechanical knob has clearly marked timer positions around it, such as OFF->10Min->20Min->30Min->60Min. It is simple, direct, and reliable to operate. The digital button is used in conjunction with the display screen to allow for more flexible time settings. Safety Design: The ultraviolet sterilization timer is connected in series with the door cover safety interlock switch in the physical circuit. Only when the door cover is completely closed can the knob be turned away from the OFF position, and only then can the ultraviolet lamp (3) be lit.

[0092] Core component 2: UV sterilization status indicator light. Type: A high-brightness purple LED or a red LED with a UV symbol. Function: The constantly lit function clearly indicates that the UV lamp (3) is working, there is strong ultraviolet light inside the container, and it is absolutely forbidden to open the sealed door (2); the extinguished function indicates that the UV lamp has been turned off.

[0093] 5. System power and status indicator area

[0094] Core Component 1: Main Power Switch. Type: A rocker switch with indicator light or a self-locking push-button switch. Function: Controls the power supply to the entire culture vessel. The switch itself is backlit (e.g., red or green) for easy location in dimly lit environments.

[0095] Core Component 2: System Status / Alarm Indicator Light. Type: One tri-color LED indicator (or multiple separate indicator lights). Function: A solid green light indicates normal system operation, in standby or incubation mode; a flashing yellow light is a general reminder, such as sterilization complete, please let it stand and wait for ozone decomposition; a flashing / solid red light accompanied by a buzzer is a serious alarm, such as temperature exceeding limits or door not properly closed.

[0096] Core Component 3: Mute / Confirm Button. Type: A touch button, labeled ALM RESET / MUTE. Function: Used to silence the buzzer when an alarm occurs and to confirm that the alarm information has been received.

[0097] Control logic and safety interlock (functional integration) of the control panel (8): These hardware components are connected into an intelligent and safe system through an internal microcontroller (such as STM32): ① Power-on process: Turn on the main power switch, the display screen lights up, performs a self-test, and displays the current temperature and status. ② Temperature setting: Press the temperature setting encoder briefly, the screen prompts the set temperature, rotate the encoder to adjust the value, and press again to confirm. ③ Sterilization process: Prerequisite: The sealed door (2) must be completely closed (triggering the door cover switch); Start: Turn the UV sterilization timer knob from OFF to the required time setting; Execution: The UV sterilization status indicator (purple) lights up immediately, the UV lamp (3) works, and the display screen shows the remaining time; End: After the timer ends, the UV lamp (3) automatically turns off, the purple indicator light turns off, the system emits a prompt sound, and the display screen may indicate that sterilization is complete and safe to operate. ④ Safety Interlock: Door-UV Interlock: As long as the sealed door (2) is opened, the power supply circuit of the UV lamp (3) will be forcibly cut off, and the lamp will not light up even if the timer knob is not in the OFF position; UV-Operation Prompt: During UV sterilization, if someone is detected trying to open the gloves of the operation interface, the system will trigger the highest level alarm.

[0098] (V) Workflow of a lung cancer cell culture vessel with ozone sterilization function

[0099] 1. Preparation stage: Sterilize the petri dishes, culture media, reagents, etc. and put them into the container through the sealed door (2).

[0100] 2. Sterilization Stage: Close the sealed door (2) and start the ultraviolet sterilization program via the control panel (8). The ultraviolet lamp (3) operates for 30 minutes, during which ozone is generated to thoroughly sterilize all items and space inside the container. After sterilization, the ultraviolet lamp (3) is turned off. Let it stand for 1-2 hours, or allow the ozone to decompose naturally / be catalytically through photocatalysis.

[0101] 3. Operation and inoculation stage: The operator digests, centrifuges (a small palm-sized centrifuge can be used to put the sample into the container), resuspends the lung cancer tissue sample through the glove hole (4), and inoculates it into a culture dish.

[0102] 4. Incubation Stage: Close the operating port and start the temperature control system. The incubator can then be used for long-term incubation on a regular laboratory table. Users can periodically change the medium, observe, or perform subculturing operations through the glove hole (4).

[0103] 5. Sterilization before reoperation: Before each opening of the sealed door (2) or before the next operation, a short ultraviolet sterilization procedure can be restarted to ensure that the system is always in a sterile state.

[0104] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present invention, and these improvements and modifications assumed above should also be considered within the scope of protection of the present invention.

Claims

1. A lung cancer cell culture vessel with ozone sterilization function, comprising a vessel body (1), a sealing door (2), an ultraviolet lamp (3), a semiconductor cooling chip (5), a temperature sensor (6), an air filter (7), a control panel (8), an electrical and functional interface (9), a water tray (10), a shelf (15), a telescopic sleeve (16), and operating gloves (17), characterized in that, The tank (1) is made of stainless steel inner liner and external insulation material, with a size of approximately 40cm long x 30cm wide x 30cm high. The tank (1) includes a glove hole (4), a rear wall (11), a side wall (12), a power cord (13), a handle (14), a bottom wall (18), and an inner cavity (19). The tank (1) has a transparent sealing door (2) at the front. An 8W UVC ultraviolet lamp tube (3) is installed on the top inside the tank. The inner wall of the tank is coated with a nano-titanium dioxide photocatalytic coating to form an ultraviolet sterilization and ozone management module. A semiconductor cooling chip (5) is integrated on the bottom wall (18), and its hot end is connected to the external heat sink and fan. A DS18B20 temperature sensor (6) is installed inside the bottom wall (18), which is connected to the control circuit based on the STM32 microcontroller. The circuit is connected, and the temperature is precisely controlled at 37±0.2℃ through PID algorithm; an air filter (7) with a 0.22μm hydrophobic filter membrane is provided on the upper part of the rear wall (11) of the tank; the semiconductor cooling chip (5), temperature sensor (6) and air filter (7) together constitute the cell culture environment maintenance system; a glove hole (4) is provided in the center of each tank side wall (12), and the operating glove (17) is connected through the telescopic sleeve (16); the control panel (8) is located on the front of the tank; the sealing door (2) is a magnetic door, which consists of a sealing door frame (20), a sealing door frame side groove (21), a sealing magnetic strip (22), a sealing door frame cavity (23), and inner and outer sealing strips (24). The door is composed of a glass door with an iron edge (25), a door handle (26), an iron edge (27), and door hinges (28). The sealed door frame (20) is made of stainless steel or aluminum alloy and is a rectangular frame with an inwardly recessed groove, i.e., the cross-section is L-shaped. The sealed door frame (20) includes a frame and a support frame. The frame is 1-2 cm long and 0.5-1 cm wide. The outer surface along the length direction is welded to the front wall of the tank (1) at multiple points, and the gaps are filled with glass glue to seal and adhere them together. The support frame is the part that extends into the cavity (23) of the sealed door frame, with a length of 2-3 cm and a width of 0.5-1 cm. The sealed door frame groove (21) is the part of the sealed door frame ( The frame and support frame of the sealed door frame (20) form a groove on the outer part of the sealed door frame (20) for connecting the iron-edged glass door (25) and the sealing magnetic strip (22). The depth of the sealed door frame side groove (21) is 0.5-1 cm. The sealing magnetic strip (22) is a long strip permanent magnet fixed in the center of the outer surface of the support frame of the sealed door frame (20). A silicone outer sleeve with an isosceles right triangle cross-section is provided outside the magnet. The outer sleeve is adhered to the outer surface of the support frame of the sealed door frame (20) by adhesive. The sealed door frame cavity (23) is the cavity enclosed by the support frame of the sealed door frame (20). The iron-edged glass door (25) is a door with an iron edge around the perimeter and a central transparent glass, with a thickness of 0.5-1 cm, connected movably to the door frame (20) via door hinges (28); door iron edge (27) is the iron edge around the iron edge glass door (25); the door iron edge (27) at the top, bottom and outer edges of the iron edge glass door (25) has a U-shaped cross-section, the U-shaped inner cavity is embedded in the glass edge of the iron edge glass door (25), while the door iron edge (27) at the inner edge of the iron edge glass door (25) has a μ-shaped cross-section, the μ-shaped inner cavity is embedded in the glass edge of the iron edge glass door (25), the μ-shaped protruding wings of the two iron edge glass doors (25) are close together, the outer surface is used to adhere and seal with the middle and outer sealing strips (24), the outside of the μ-shaped protruding wings forms a groove with a depth of 0.5-1 cm and a width of 0.5-1 cm; the middle and outer sealing strips (24) are made of rubber or plastic, with a T-shaped cross-section, and are divided into handles. The door frame consists of two parts: a handle strip and an embedded strip. The length of the outer sealing strip (24) is the same as the length of the iron-edged glass door (25). The handle strip is 2-3 cm wide and 2-3 cm thick. The embedded strip is 0.5-1 cm thick and 0.5-1 cm wide. One outer surface is connected to the inner surface of the handle strip to form a whole and is located in the center of the inner surface of the handle strip. Two sealing magnetic strips (22) are provided on the opposite surface. The door handle (26) is made of stainless steel or aluminum alloy and has the same structure as known door handles. It is set on the inner edge of the iron-edged glass door (25) and the connector is equipped with a sealing gasket. The door hinge (28) has the same structure and function as known door hinges. It is made of stainless steel and is connected between the iron-edged glass door (25) and the sealed door frame (20) to form a movable connection between the iron-edged glass door (25) and the sealed door frame (20).

2. The lung cancer cell culture vessel with ozone sterilization function according to claim 1, characterized in that, The inner wall of the tank (1) is coated with a photocatalytic material.

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