A composition for improving blood vessel aging and a preparation method and application thereof

Abstract

The present application relates to a kind of improving vascular senility composition and its preparation method and application, belong to natural product technical field.The present application provides a kind of improving vascular senility composition, including osmanthus fragrans extract and pumpkin seed protein peptide, the mass ratio of osmanthus fragrans extract and pumpkin seed protein peptide is osmanthus fragrans extract:pumpkin seed protein peptide=1:(0.2-8).The present application is improved vascular senility composition by osmanthus fragrans extract and pumpkin seed protein peptide collocation, can effectively inhibit angiotensin II induced vasoconstriction, enhance cardiac output, prevent zebra fish heart structure and function damage, while also can enhance blood flow velocity, reduce vascular injury, from multidimensional synergistic prevention vascular senility formation.

Description

Technical Field

[0001] This invention relates to the field of natural product technology, and in particular to a composition for improving vascular aging, its preparation method, and its application. Background Technology

[0002] Vascular aging refers to the process of vascular structural remodeling and functional decline caused by factors such as age, environment, and inflammatory stimulation. It is not only a significant factor contributing to cardiovascular disease (CVD) in the elderly but also an independent risk factor. Controlling and delaying vascular aging is crucial for preventing age-related CVD and improving its prognosis. "Prevention" and "gateway shifting" are the core of addressing this problem.

[0003] Angiotensin II is the main effector of CVD mediated by the renin-angiotensin-aldosterone system. Excessive stimulation of angiotensin II leads to various pathological manifestations such as vascular endothelial dysfunction, oxidative stress, myocardial and vascular remodeling, and inflammatory reactions. These pathological damages together promote the formation of vascular aging.

[0004] Addressing the sub-healthy state of vascular aging induced by modern lifestyles, nutritional intervention to inhibit early vascular lesions and restore physiological function has become an urgent need and core strategy for developing highly effective and safe vascular health compositions. However, health foods specifically designed to improve vascular aging are currently very rare. Furthermore, most existing anti-vascular aging ingredients act through a single mechanism, and compositions often lack a clearly defined mechanism of action.

[0005] Therefore, there is an urgent need to develop a highly efficient and safe composition that promotes vascular health. Summary of the Invention

[0006] The purpose of this invention is to overcome the shortcomings of the prior art and provide a composition for improving vascular aging, its preparation method, and its application.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a composition for improving vascular aging, comprising osmanthus extract and pumpkin seed protein peptide, wherein the mass ratio of osmanthus extract to pumpkin seed protein peptide is osmanthus extract: pumpkin seed protein peptide = 1: (0.2-8).

[0008] This invention combines osmanthus extract and pumpkin seed protein peptides to create a composition that improves vascular aging. This composition effectively inhibits angiotensin II-induced vasoconstriction, enhances cardiac output, and prevents structural and functional damage to the zebrafish heart. It also increases blood flow velocity and reduces vascular damage, thus synergistically preventing vascular aging from multiple dimensions. Osmanthus extract (rich in rhodioloside and ligustrazine) has the core advantage of powerful antioxidant (activating the Nrf2 pathway) and anti-inflammatory (inhibiting the NF-κB pathway) capabilities, clearing upstream "inducing factors" that lead to vascular damage. Pumpkin seed protein peptides, as "repair agents," have the core advantage of improving vascular endothelial function and promoting endothelial repair. The combined effect of these two ingredients on improving vascular aging far surpasses that of any single-mechanism ingredient.

[0009] As a preferred embodiment of the composition for improving vascular aging described in this invention, the mass ratio of osmanthus extract to pumpkin seed protein peptide is osmanthus extract: pumpkin seed protein peptide = 1: (0.33-6).

[0010] As a preferred embodiment of the composition for improving vascular aging described in this invention, the mass ratio of osmanthus extract to pumpkin seed protein peptide is osmanthus extract: pumpkin seed protein peptide = 1: (1-5).

[0011] In a preferred embodiment of the vascular aging improvement composition of the present invention, the mass ratio of osmanthus extract to pumpkin seed protein peptide is osmanthus extract: pumpkin seed protein peptide = 1:3. The present invention has experimentally demonstrated that when the mass ratio of osmanthus extract to pumpkin seed protein peptide is 1:3, the composition exhibits the best vascular aging improvement effect.

[0012] As a preferred embodiment of the composition for improving vascular aging described in this invention, the osmanthus extract contains rhodioloside and ligustrum lucidum glycoside, wherein the mass percentage of rhodioloside in the osmanthus extract is ≥2.0% and the mass percentage of ligustrum lucidum glycoside in the osmanthus extract is ≥0.8%.

[0013] In a preferred embodiment of the composition for improving vascular aging according to the present invention, the osmanthus extract is mainly obtained by heating and extracting osmanthus flowers, water, and acidic fruit juice. Preferably, the osmanthus flowers are either edible Osmanthus fragrans or Osmanthus fragrans var. aurantiacus.

[0014] As a preferred embodiment of the composition for improving vascular aging according to the present invention, the preparation method of the osmanthus extract includes the following steps: A1. Mix osmanthus flowers with water and concentrated lemon juice, heat at 85-100℃ for 1-3 hours, and filter to obtain the first filtrate and residue; A2. Mix the first filter residue obtained in step A1 with water and concentrated lemon juice, heat and extract at 85-100℃ for 1-3 hours, and filter to obtain the second filtrate; A3. Combine the first filtrate obtained in step A1 and the second filtrate obtained in step A2, concentrate and dry to obtain osmanthus extract.

[0015] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in step A1, the mass ratio of osmanthus, water and concentrated lemon juice is osmanthus:water:concentrated lemon juice = 1:(12-18):(0.02-0.06).

[0016] As a preferred embodiment of the composition for improving vascular aging described in this invention, in step A1, the mass ratio of osmanthus, water and concentrated lemon juice is osmanthus:water:concentrated lemon juice = 1:(12-15):(0.03-0.05).

[0017] As a preferred embodiment of the composition for improving vascular aging described in this invention, in step A1, the mass ratio of osmanthus, water and concentrated lemon juice is osmanthus:water:concentrated lemon juice = 1:15:0.05.

[0018] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in step A2, the mass ratio of the filter residue, water and concentrated lemon juice is filter residue: water: concentrated lemon juice = 1: (8-12): (0.02-0.06).

[0019] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in step A2, the mass ratio of the filter residue, water and concentrated lemon juice is filter residue: water: concentrated lemon juice = 1: (10-12): (0.03-0.05).

[0020] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in step A2, the mass ratio of the filter residue, water and concentrated lemon juice is filter residue: water: concentrated lemon juice = 1:10:0.03.

[0021] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in steps A1 and A2, the heating extraction conditions are heating and extraction at 90°C for 2 hours.

[0022] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in steps A1 and A2, the filtration is performed through a 100-400 mesh filter cloth.

[0023] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in steps A1 and A2, the total acid content in the concentrated lemon juice is 29-34% by mass.

[0024] As a preferred embodiment of the composition for improving vascular aging described in this invention, in step A3, after the concentration treatment, excipients are added for preparation and then dried.

[0025] As a preferred embodiment of the composition for improving vascular aging according to the present invention, the excipients include, but are not limited to, at least one of maltodextrin, resistant dextrin, α-cyclodextrin and polydextrin.

[0026] In a preferred embodiment of the composition for improving vascular aging described in this invention, the amount of excipients added is 10-40 wt% of the total mass of the concentrate.

[0027] The pumpkin seed protein peptides selected in this invention have a strong ability to scavenge peroxide free radicals, which can remove ROS in cells and reduce oxidative stress. They also have an ameliorative effect on vascular endothelial cell dysfunction caused by oxidative stress, can significantly increase NO production, reduce inflammatory factor levels, and fundamentally improve vascular endothelial cell dysfunction.

[0028] As a preferred embodiment of the composition for improving vascular aging described in this invention, the pumpkin seed protein peptide is mainly obtained by enzymatic hydrolysis of crude pumpkin seed protein by a complex protease. The complex protease includes alkaline protease and trypsin, and the mass ratio of alkaline protease to trypsin in the complex protease is alkaline protease:trypsin = (1-3):(1-3).

[0029] As a preferred embodiment of the composition for improving vascular aging according to the present invention, the method for preparing the pumpkin seed protein peptide includes the following steps: B1. Add a complex protease to the homogenized slurry of crude pumpkin seed protein for enzymatic hydrolysis to obtain the hydrolysate; B2. The enzyme hydrolysate obtained in step B1 is heated to inactivate the enzyme, centrifuged to collect the supernatant, concentrated and dried to obtain pumpkin seed protein peptides.

[0030] As a preferred embodiment of the composition for improving vascular aging described in this invention, in step B1, the pumpkin seed crude protein homogenate is obtained by adding pumpkin seed crude protein to water and homogenizing it, and the mass ratio of pumpkin seed crude protein to water is pumpkin seed crude protein:water = 1:(7.33-15.67).

[0031] As a preferred embodiment of the composition for improving vascular aging described in this invention, in step B1, the pumpkin seed crude protein homogenate is obtained by adding pumpkin seed crude protein to water and homogenizing it, and the mass ratio of pumpkin seed crude protein to water is pumpkin seed crude protein: water = 1:9.

[0032] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in step B1, the complex protease includes alkaline protease and trypsin, and the mass ratio of alkaline protease to trypsin in the complex protease is alkaline protease:trypsin = (1-3):(1-3).

[0033] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in step B1, the mass ratio of alkaline protease and trypsin in the complex protease is alkaline protease:trypsin = 1:1.

[0034] As a preferred embodiment of the composition for improving vascular aging described in this invention, in step B1, the amount of the complex protease added is 0.2-1.1 wt% of the total mass of pumpkin seed crude protein in the pumpkin seed crude protein homogenate.

[0035] As a preferred embodiment of the composition for improving vascular aging described in this invention, in step B1, the amount of the complex protease added is 0.9 wt% of the total mass of pumpkin seed crude protein in the pumpkin seed crude protein homogenate.

[0036] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in step B1, the enzymatic hydrolysis conditions are 48-57°C and pH=7.5-10 for 3-6 hours.

[0037] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in step B1, the enzymatic hydrolysis conditions are 50-54°C and pH=8.0-9.0 for 5 hours.

[0038] As a preferred embodiment of the composition for improving vascular aging according to the present invention, in step B2, the enzyme inactivation by heating is performed by heating at 100°C for 30 min; the centrifugation is performed by centrifugation at 8000 r / min for 10 min.

[0039] Secondly, the present invention provides a method for preparing the above-mentioned composition for improving vascular aging, comprising the following steps: Pumpkin seed protein peptides and osmanthus extract were mixed to obtain a composition for improving vascular aging.

[0040] Thirdly, the present invention provides the application of the above-mentioned composition for improving vascular aging in the preparation of products for improving vascular aging.

[0041] In a preferred embodiment of the application described in this invention, the product includes at least one of food, pharmaceutical, and cosmetic products. This invention uses edible osmanthus flowers, concentrated fruit juice, and water as raw materials to prepare osmanthus extract, which, when combined with equally edible pumpkin seed protein peptides, exhibits high safety.

[0042] Fourthly, the present invention provides a product comprising the above-described composition for improving vascular aging.

[0043] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention combines pumpkin seed protein peptides and osmanthus extract to create a composition for improving vascular aging. It combines the two mechanisms of action of pumpkin seed protein peptides in improving vascular endothelial function and osmanthus extract in enhancing hemodynamics, thereby achieving synergistic effects in improving vasodilation, cardiac output, and blood flow velocity, and thus synergistically improving vascular aging. Attached Figure Description

[0044] Figure 1 A diagram of the vascular-improving composition product obtained in Example 1 of the present invention; Figure 2 This illustrates the effect of different treatments on the diameter of blood vessels in zebrafish in Example 1 of the present invention. Figure 3 This illustrates the effect of different treatments on the blood flow velocity of zebrafish in Example 1 of the present invention. Figure 4 The effect of different treatments on the output of zebrafish hearts is shown in Example 1 of the present invention. Detailed Implementation

[0045] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0046] Unless otherwise specified, all other materials and reagents used in the examples, comparative examples, and effect examples are commercially available.

[0047] The INCI names of the cosmetic ingredients mentioned below are shown in Table 1, and all of them are commercially available.

[0048] The pumpkin seed protein peptide 1 mentioned below is mainly obtained by enzymatic hydrolysis of crude pumpkin seed protein with a complex protease, wherein the complex protease includes alkaline protease and trypsin, and the mass ratio of alkaline protease to trypsin in the complex protease is alkaline protease:trypsin = (1-3):(1-3), which is carried out in accordance with paragraphs

[0057] -

[0060] of the specification of CN 119177268 A, specifically including the following operations: 1) Add 1 kg of pumpkin seed crude protein to 9 kg of aqueous solution, stir evenly and then homogenize to obtain a homogenate.

[0049] 2) The pH of the homogenate was adjusted to 8.5±0.5 with 5mol / L sodium hydroxide, and then 4.5g alkaline protease and 4.5g trypsin were added. The mixture was enzymatically hydrolyzed for 5h at 52±2℃ to obtain the hydrolysate.

[0050] 3) The enzyme hydrolysate was heated to 100℃ and held for 30 minutes to inactivate the enzyme. After enzyme inactivation, the solution was centrifuged at 8000 r / min for 10 minutes, and the supernatant was collected, concentrated, and dried to obtain pumpkin seed protein peptide 1.

[0051] The pumpkin seed protein peptide 2 mentioned below is mainly obtained by alkaline protease hydrolysis of crude pumpkin seed protein, as described in sections

[0077] -

[0080] of the specification CN 119177268 A, specifically including the following operations: 1) Add 1 kg of pumpkin seed crude protein to 9 kg of aqueous solution, stir evenly and then homogenize to obtain a homogenate.

[0052] 2) The pH of the homogenate was adjusted to 8.5±0.5 with 5mol / L sodium hydroxide, and then 9g of alkaline protease was added. The homogenate was hydrolyzed for 5h at 52±2℃ to obtain the hydrolysate.

[0053] 3) The enzyme hydrolysate was heated to 100℃ and kept at that temperature for 30 minutes to inactivate the enzyme. After inactivation, the solution was centrifuged at 8000 rpm for 10 minutes, and the supernatant was collected, concentrated, and dried to obtain pumpkin seed protein peptide 2.

[0054] The crude protein in the pumpkin seeds mentioned above comes from Baoderui (Hubei) Health Industry Co., Ltd., with a protein content of approximately 60%.

[0055] In the following examples and comparative examples, the osmanthus extract raw material, osmanthus flowers, was purchased from Green Source Agricultural Products Co., Ltd. The osmanthus flowers were dried flowers and obtained by microwave vacuum drying; the concentrated lemon juice was purchased from Dele Food & Beverage Ingredients (Rizhao) Co., Ltd., and the total acid content in the concentrated lemon juice was 29-34% by mass.

[0056] Examples 1-4 and Comparative Examples 1-4 Examples 1-4 and Comparative Examples 1-4 respectively provide a composition for improving vascular aging and its preparation method. The composition for improving vascular aging includes pumpkin seed protein peptide 1 and osmanthus extract, with the mass ratio of the two shown in Table 1. The preparation method involves mixing pumpkin seed protein peptide 1 and osmanthus extract. The composition for improving vascular aging obtained in Example 1 is as follows: Figure 1 As shown.

[0057] The preparation method of the osmanthus extract includes the following steps: S1. Mix osmanthus, water and concentrated lemon juice according to the mass ratio of osmanthus: water: concentrated lemon juice = 1:15:0.05, heat at 90℃ for 2 hours, and filter through a 100-mesh filter cloth to obtain the first filtrate and filter residue. S2. Mix the first filter residue obtained in step S1, water and concentrated lemon juice according to the mass ratio of filter residue: water: concentrated lemon juice = 1:10:0.03, heat and extract at 90°C for 1 hour, and filter to obtain the second filtrate. S3. The first filtrate obtained in step S1 and the second filtrate obtained in step S2 are combined and concentrated under reduced pressure at 60-75℃ to obtain a concentrate. Resistant dextrin is added, with the amount of resistant dextrin added being 30wt% of the total mass of the concentrate. The concentrate is then spray-dried and passed through a 40-mesh sieve to obtain osmanthus extract. HPLC analysis shows that the osmanthus extract contains 3.29% rhodioloside and 0.92% ligustrol.

[0058] Table 1. Mass ratio of each component in different compositions for improving vascular aging Comparative Example 5 Comparative Example 5 provides a composition for improving vascular aging and its preparation method. The components and their amounts in the composition for improving vascular aging are similar to those in Example 1, except that pumpkin seed protein peptide 1 is replaced with pumpkin seed protein peptide 2. The preparation method is the same as in Example 1, and the remaining components and their amounts remain unchanged.

[0059] Comparative Example 6 Comparative Example 6 provides a composition for improving vascular aging and its preparation method. The components and their amounts in the composition for improving vascular aging are similar to those in Example 1, except that the osmanthus extract is replaced with Rhodiola rosea extract. The preparation method is the same as in Example 1, and the remaining components and their amounts remain unchanged.

[0060] Example of effect 1 To verify the vascular aging improvement effect of the compositions obtained in Examples 1-4 and Comparative Examples 1-6, a zebrafish vascular aging model was constructed for experimentation. The specific scheme is as follows: Angiotensin II (AngII) can activate the renin-angiotensin-aldosterone (RAAS) system. Excessive stimulation by AngII leads to various pathological manifestations, including endothelial dysfunction, oxidative stress, myocardial and vascular remodeling, and inflammatory responses. These pathological damages collectively promote vascular aging. Angiotensin II is a potent vasoconstrictor that induces vasoconstriction in zebrafish, causing changes in the structure and function of the zebrafish heart, reducing cardiac output, and simultaneously decreasing blood flow velocity (mean blood flow velocity) due to increased vascular resistance. Therefore, this experiment used AngII as the modeling agent for a zebrafish vascular aging model, with vessel diameter (D), blood flow velocity (V), and cardiac output (T) as evaluation indicators.

[0061] The zebrafish used in this experiment were transgenic fluorescent vascular endothelial zebrafish (green in color). Due to the characteristics of transgenic zebrafish, changes in blood vessels and blood flow can be clearly observed. Zebrafish of normal size, capable of normal suspension and swimming, and of similar body length, 3 days post-fertilization, were selected and placed in containers made of inert material. The maximum zebrafish load was no more than 10 fish / mL. Angiotensin II was used to establish the vascular aging model. After successful modeling, the solution in the 6-well plates was removed without harming the zebrafish. Some zebrafish were fixed with 3.7% methylcellulose. Fluorescence images of the intestinal vessels of the zebrafish were observed and photographed under a fluorescence microscope. During photography, the zebrafish were positioned with one side facing down and the body kept horizontal. All zebrafish photographs were taken under the same instruments and conditions, and the zebrafish positions were kept consistent. Ten zebrafish were randomly selected from each experimental group and placed in a blood flow analyzer for video recording and data collection. Blood flow velocity (V), vessel diameter (D), and cardiac output were analyzed.

[0062] Experimental group setup: A total of 13 groups were set up, including 1 blank group, 1 model group, 1 positive control group, and 10 sample groups. Blank group: embryo culture medium; Model group: 2 μM Ang II solution; Positive control group: 10 μM valsartan solution + 2 μM Ang II solution; Sample group: 62.5 μg / mL sample + 2 μM Ang II solution. Each group consisted of 15 zebrafish, and the culture medium per well was 3 mL.

[0063] Data statistics: 8-10 zebrafish were randomly selected from each group and observed under a microscope equipped with a blood flow analyzer. Videos were recorded for 20 seconds and saved to statistically analyze the blood vessel diameter, cardiac output, and blood flow velocity of each group. The average value and standard error of each group were calculated, and the percentage improvement in blood vessel diameter, blood flow velocity, and cardiac output were also calculated. The results are shown in Tables 2-4 and 2-4. Figure 2-4 The calculation formula is as follows: Table 2. Effects of different compositions for improving vascular aging on blood vessel diameter in zebrafish.

[0064] As shown in Table 2 and Figure 2The results showed a significant difference between the model group and the blank group, indicating successful modeling. Compared with the model group, Examples 1-4 all improved blood vessel diameter, while Comparative Examples 1-2 showed no significant difference. This indicates that the combination of pumpkin seed protein peptide and osmanthus extract in a specific ratio has a vasodilatory effect. While improvements were observed when the ratio was exceeded compared to the model group, they did not reach a significant level. The ratio of pumpkin seed protein peptide to osmanthus extract at 3:1 (Example 1) showed the most significant effect in vasodilating, with a blood vessel diameter improvement rate of 27.91%, comparable to the positive group. The ratios of 1:1 (Example 2) and 0.33:1 (Example 3) showed the next best effects, with blood vessel diameter improvement rates of 20.57% and 21.80%, respectively.

[0065] Using pumpkin seed protein peptides alone (Comparative Example 3) or osmanthus extract alone (Comparative Example 4) did not result in significant differences compared to the model group. Compared to Example 1, both groups showed significant differences. P <0.05), indicating that the combination of pumpkin seed protein peptide and osmanthus extract in a specific ratio can further improve the vascular diameter improvement effect of the composition.

[0066] The same compound was prepared in the same proportion as in Example 1, but was replaced with pumpkin seed protein peptide 2 (Comparative Example 5) and Rhodiola rosea extract (main component rhodioloside) (Comparative Example 6). Neither group showed significant differences compared to the model group, but both groups showed significant differences compared to Example 1. P <0.05), indicating that the composition of the present invention requires a specific type of pumpkin seed protein peptide to be combined with osmanthus extract to achieve excellent vasodilatory effects. The osmanthus extract prepared by the present invention not only contains rhodioloside but also contains active ingredients such as ligustrum lucidum glycoside, exhibiting multi-component and multi-target effects.

[0067] Table 3. Effects of different compositions for improving vascular aging on blood flow velocity in zebrafish.

[0068] As shown in Table 3 and Figure 3 As shown, all compound groups exhibited significant improvement in blood flow velocity, with marked differences in the intensity of the effect. Examples 1-3 showed the best results, with improvement rates exceeding 50%, all higher than the positive control group. This indicates that a compound ratio of pumpkin seed protein peptide to osmanthus extract of (0.33-3):1 has a strong effect on improving blood flow velocity. When the compound ratio of pumpkin seed protein peptide to osmanthus extract was (0.11-0.2):1 or 9:1 (i.e., Example 4, Comparative Examples 1-2), although blood flow velocity was improved, the effect was significantly reduced compared to Examples 1-3. Specifically, Comparative Example 2 (9:1) showed a 77.6% decrease in improvement rate compared to Example 3 (0.33:1).

[0069] Pumpkin seed protein peptide alone (Comparative Example 3) was ineffective in improving blood flow velocity. Osmanthus extract alone (Comparative Example 4) did improve blood flow velocity, but when combined with pumpkin seed protein peptide, it further improved blood flow and enhanced the effect of using it alone. Compared with Comparative Example 4, Example 3 showed a 60% increase in the effect of improving blood flow.

[0070] Although the compound was prepared in the same proportion as in Example 1, replacing it with Rhodiola rosea extract (Comparative Example 6) had the effect of improving blood flow velocity, the improvement rate decreased by 67.6% compared with Example 1.

[0071] The above data shows that although osmanthus extract can improve blood flow velocity, it can achieve a synergistic effect when combined with pumpkin seed protein peptides in a better mass ratio.

[0072] Table 4. Effects of different compositions for improving vascular aging on cardiac output in zebrafish.

[0073] As shown in Table 4 and Figure 4 As shown, all experimental groups showed improvement in cardiac output. However, Example 1 demonstrated a clear leading advantage, followed by Examples 3 and 2, with all showing better improvement than Comparative Examples 1-6. Specifically, Example 1 showed a 53.1% improvement over Comparative Example 3 and a 50.5% improvement over Comparative Example 4, indicating that the combination of pumpkin seed protein peptides and osmanthus extract can synergistically enhance the effect of using them alone.

[0074] The same compound was prepared in the same proportion as in Example 1, and replaced with pumpkin seed protein peptide 2 (Comparative Example 5) and Rhodiola rosea extract (Comparative Example 6). Although both groups showed significant differences compared with the model group, the improvement effect was significantly lower than that of Examples 1-3. Among them, the improvement effect of Comparative Examples 5-6 decreased by 59.7% and 47.7% respectively compared with Example 1.

[0075] In conclusion, the combination of pumpkin seed protein peptides and osmanthus extract exhibits synergistic effects, both superior to their individual uses. Within a specific mass ratio range, both demonstrate vasodilatory effects, improving blood flow velocity and cardiac output. The optimal mass ratio of pumpkin seed protein peptides to osmanthus extract is 0.33:1, resulting in the best effect on improving blood flow velocity; while a mass ratio of 3:1 shows the best effects on vasodilatory and cardiac output-enhancing effects.

[0076] This also corresponds to the core mechanism of the raw materials. The mechanism of action of pumpkin seed protein peptides is mainly to dilate blood vessels, reduce cardiac load, and increase cardiac output by improving vascular endothelial function; the mechanism of action of osmanthus extract is mainly to reduce cardiac load and dilate blood vessels by enhancing hemodynamics.

[0077] The combined use of these two agents not only inhibits angiotensin II-induced vasoconstriction, increases cardiac output, and prevents structural and functional damage to the zebrafish heart, but also enhances blood flow velocity, reduces vascular damage, and prevents vascular aging from multiple dimensions.

[0078] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for improving vascular aging, characterized in that, It includes osmanthus extract and pumpkin seed protein peptide, wherein the mass ratio of osmanthus extract to pumpkin seed protein peptide is osmanthus extract: pumpkin seed protein peptide = 1: (0.2-8).

2. The composition for improving vascular aging as described in claim 1, characterized in that, Includes at least one of the following (I)-(II): (I) The osmanthus extract contains rhodioloside and privetin, wherein the mass percentage of rhodioloside in the osmanthus extract is ≥2.0%, and the mass percentage of privetin in the osmanthus extract is ≥0.8%; (II) The osmanthus extract is mainly obtained by heating and extracting osmanthus, water and acidic fruit juice.

3. The composition for improving vascular aging as described in claim 1 or 2, characterized in that, The preparation method of the osmanthus extract includes the following steps: A1. Mix osmanthus flowers with water and concentrated lemon juice, heat at 85-100℃ for 1-3 hours, and filter to obtain the first filtrate and residue; A2. Mix the first filter residue obtained in step A1 with water and concentrated lemon juice, heat and extract at 85-100℃ for 1-3 hours, and filter to obtain the second filtrate; A3. Combine the first filtrate obtained in step A1 and the second filtrate obtained in step A2, concentrate and dry to obtain osmanthus extract.

4. The composition for improving vascular aging as described in claim 3, characterized in that, Includes at least one of the following (III)-(V): (III) In step A1, the mass ratio of osmanthus, water and concentrated lemon juice is osmanthus:water:concentrated lemon juice = 1:(12-18):(0.02-0.06); (IV) In step A2, the mass ratio of the filter residue, water, and concentrated lemon juice is filter residue:water:concentrated lemon juice = 1:(8-12):(0.02-0.06). (V) In step A3, after the concentration treatment, the auxiliary materials are added and then dried.

5. The composition for improving vascular aging as described in claim 1, characterized in that, The pumpkin seed protein peptide has a peptide content of ≥50% by mass, and the number of peptides with a relative molecular mass of 189-6000 Da accounts for 60% or more of the total number of peptides in the pumpkin seed protein peptide.

6. The composition for improving vascular aging as described in claim 1, characterized in that, The pumpkin seed protein peptide is mainly obtained by enzymatic hydrolysis of crude pumpkin seed protein by a complex protease. The complex protease includes alkaline protease and trypsin, and the mass ratio of alkaline protease to trypsin in the complex protease is alkaline protease:trypsin = (1-3):(1-3).

7. The method for preparing the composition for improving vascular aging according to any one of claims 1-6, characterized in that, Includes the following steps: Pumpkin seed protein peptides and osmanthus extract were mixed to obtain a composition for improving vascular aging.

8. The use of the composition for improving vascular aging as described in any one of claims 1-6 in the preparation of products for improving vascular aging.

9. The application as described in claim 8, characterized in that, The products include at least one of food, medicine, and cosmetics.

10. A product characterized in that, Includes the vascular aging improvement composition according to any one of claims 1-6.

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