Method for separating and extracting astaxanthin by taking haematococcus pluvialis as raw material

By using a combination of natural eutectic solvents and antioxidants, the problems of solvent residue and low cell wall disruption efficiency in the extraction of astaxanthin from Haematococcus pluvialis were solved, achieving efficient and green astaxanthin extraction and purification, improving extraction rate and purity, and inhibiting oxidative degradation and isomerization.

CN122102992APending Publication Date: 2026-05-29ERFA BIOTECHNOLOGY (JIAXING) CO LTD
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Patent Information

Application Number
CN202610545938.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-23
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing technologies for extracting astaxanthin from Haematococcus pluvialis suffer from problems such as excessive solvent residue, low cell wall disruption efficiency, high equipment costs, significant safety risks, fragmented extraction and purification separation steps, and low purity of the target product, making it difficult to achieve green, efficient, and highly selective integrated extraction and purification.

Method used

By using a natural eutectic solvent (composed of betaine, natural organic acids and D-glucose) combined with tocopherol and ascorbyl palmitate, and through steps such as ultrasonic stirring, centrifugation, resin column purification and freeze drying, a synergistic effect of "cell wall disruption-dissolution-stabilization" is constructed to improve the extraction rate and purity of astaxanthin and inhibit oxidative degradation and cis isomerization.

Benefits of technology

It has achieved efficient extraction of astaxanthin from Haematococcus pluvialis with an extraction rate of over 94%, a purity of over 98%, an all-trans astaxanthin ratio of over 93%, and an antioxidant retention rate of over 94%, without the need for the addition of functionalized nanomaterials or chemical modifiers.

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Abstract

The invention relates to the technical field of separation and extraction of natural products, and particularly discloses a method for separating and extracting astaxanthin by taking haematococcus pluvialis as a raw material, which comprises the following steps: (1) pretreating haematococcus pluvialis to prepare haematococcus pluvialis dry powder; (2) separating and extracting astaxanthin from the haematococcus pluvialis dry powder by adopting an ultrasonic-assisted natural eutectic solvent to obtain a crude extract; (3) dynamically adsorbing the crude extract by adopting macroporous resin, purifying and collecting an astaxanthin eluent; and (4) performing rotary evaporation and concentration on the astaxanthin eluent under reduced pressure, and performing freeze drying to obtain astaxanthin crystal powder. According to the method provided by the invention, through ternary synergy of the natural eutectic solvent and cross-phase antioxidant synergy of tocopherol and ascorbyl palmitate, and by matching with a mild process of ultrasonic-assisted extraction and resin gradient elution, the extraction rate of the astaxanthin is effectively improved, the purity of the astaxanthin and the proportion of all-trans astaxanthin are kept, and the purity of the astaxanthin is improved. The antioxidant retention time of the astaxanthin is prolonged.
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