Fluid transport method, system and biochemical substance analysis method

Abstract

A fluid transportation method, system and biochemical substance analysis method for transporting a plurality of fluids to a fluid using system. The system includes a fluid transportation body having a plurality of introduction channels and a merging channel. The plurality of introduction channels includes a first introduction channel for introducing a first type of fluid and a second introduction channel other than the first introduction channel. The first type of fluid is a fluid that is cross-contaminated or easily influenced when mixed with at least one other fluid of the plurality of fluids. The first introduction channel and the second introduction channel merge at a first merging point, and the plurality of introduction channels merge at a second merging point downstream of the first merging point.

Description

Technical Field

[0001] This application relates to fluid control, and more particularly to a fluid transport method, system, and method for analyzing biochemical substances. Background Technology

[0002] In fields such as biology, chemistry, and medicine, common instruments are designed based on the core principles of biological or chemical reactions. The substances involved in biological or chemical reactions (such as reagents) are typically in a liquid or gaseous state in physics, collectively referred to as fluids. To achieve the biological or chemical reactions required by the instrument, it usually needs to use containers as reaction chambers for samples and fluids, and the instrument also needs to have a transport system to deliver different fluids into the reaction chambers.

[0003] In related technologies, the transport system includes reagent kits loaded with different reagents and valves for sorting reagents. When a valve is open, the corresponding reagent kit is fluidly connected to the reaction tank through a flow channel, allowing the reagent in the kit to flow into the reaction tank. However, since the downstream of multiple flow channels converges into a single confluence channel to connect to the reaction tank, when the reagent in a reagent kit flows into the reaction tank through its corresponding flow channel, the reagent may experience overflow or over-flushing at the confluence point, resulting in the reagent remaining in other flow channels near the confluence point. Therefore, when other reagent kits are connected to the reaction tank, the introduced reagent may mix with this residual reagent, causing cross-contamination. This mixture entering the reaction tank may also affect the biochemical reaction or detection results within the reaction tank. Summary of the Invention

[0004] This application aims to propose a fluid transport method, system, and biochemical analysis method to improve the problem of cross-contamination during the transport of multiple fluids.

[0005] This application provides a fluid transport system for transporting multiple fluids to a fluid usage system. The fluid transport system includes a fluid transport body with multiple inlet channels and a confluence channel. The multiple inlet channels include a first inlet channel for introducing a first type of fluid and a second inlet channel other than the first inlet channel. The first type of fluid is a fluid that would cause cross-contamination or easily influence each other when mixed with at least one other fluid among the multiple fluids. A first inlet channel and a second inlet channel converge at a primary confluence point, and the multiple inlet channels converge at a secondary confluence point located downstream of the primary confluence point.

[0006] A second aspect of this application provides a fluid transport method for transporting multiple fluids to a fluid usage system. The fluid transport method includes: providing the aforementioned fluid transport system; connecting multiple inlet channels of the fluid transport system to multiple fluid storage units, the fluid storage module including multiple fluid storage units for storing multiple fluids, the multiple fluids including a first type of fluid, the first type of fluid being fluids that would cause cross-contamination or easily influence each other when mixed with at least one other fluid among the multiple fluids; a first inlet channel connected to the fluid storage unit for storing the first type of fluid; connecting a confluence channel of the fluid transport system to a fluid usage system; sequentially connecting the multiple fluid storage units to the confluence channel; and driving the fluids within the fluid storage units to be transferred to the fluid usage system via corresponding first or second inlet channels.

[0007] A third aspect of this application provides a method for analyzing biochemical substances, comprising: performing the above-described fluid transport method to cause multiple fluids to react biochemically with a sample within a fluid use system; and performing signal detection on the reacted sample to obtain a signal that can represent the biological characteristics of the sample.

[0008] In this application, a first inlet channel intersects with a second inlet channel. Therefore, when a first type of fluid (such as a sensitive reagent) is introduced into the manifold through the first inlet channel, if the first type of fluid experiences overflow or overrush at the primary manifold point, the fluid formed by the overflow or overrush will exist in the second inlet channel, that is, in the channel used to introduce the second type of fluid (such as a non-sensitive reagent). Therefore, when the second type of fluid is subsequently introduced into the manifold through the second inlet channel, even if the second type of fluid mixes with the fluid formed by the overflow or overrush, there will be no cross-contamination or mutual influence. This also improves the situation where two fluids with cross-contamination risks are introduced into the fluid use system at the same time, causing the biochemical reaction to not proceed as expected. This facilitates the biochemical reaction and improves the detection accuracy. Attached Figure Description

[0009] Figure 1 This is a schematic diagram of the structure of a fluid transport system provided in one embodiment of this application.

[0010] Figure 2 for Figure 1 The diagram shows the flow path of a fluid transport system when transporting a fluid.

[0011] Figure 3 for Figure 1 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0012] Figure 4 for Figure 1 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0013] Figure 5 for Figure 1 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0014] Figure 6 for Figure 1 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0015] Figure 7 for Figure 1 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0016] Figure 8 This is a schematic diagram of the structure of a fluid transport system provided in another embodiment of this application.

[0017] Figure 9 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting a fluid.

[0018] Figure 10 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0019] Figure 11 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0020] Figure 12 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0021] Figure 13 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0022] Figure 14 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0023] Figure 15 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0024] Figure 16 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0025] Figure 17 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0026] Figure 18 for Figure 8The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0027] Figure 19 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0028] Figure 20 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0029] Figure 21 for Figure 8 The diagram shows the flow path of a fluid transport system when transporting another fluid.

[0030] The following detailed description, in conjunction with the accompanying drawings, will further illustrate this application. Detailed Implementation

[0031] To better understand the above-mentioned objectives, features, and advantages of this application, the application will be described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in the embodiments of this application can be combined with each other. Many specific details are set forth in the following description to provide a thorough understanding of this application; the described embodiments are only some embodiments of this application, and not all embodiments.

[0032] In the description of this application, it should be understood that the terms "upper," "lower," "front," "rear," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include one or more of the stated features. In the description of this application, it should be noted that "multiple" means two or more, unless otherwise explicitly specified.

[0033] In the description of this application, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between the components; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication between two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0034] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments.

[0035] It should be noted that when a component is described as "fixed to" or "mounted to" another component, it can be directly on the other component or may be interspersed with an intermediate component. When a component is described as "set to" another component, it can be directly set on the other component or may be interspersed with an intermediate component. The term "and / or" as used herein includes all and any combination of one or more of the associated listed items.

[0036] It should be noted that although a logical order is shown in the flowchart, in some cases, the steps shown or described may be performed in a different order than that shown in the flowchart. The methods disclosed in the embodiments of this application include one or more steps or actions for implementing the method. Method steps and / or actions may be interchanged with each other without departing from the scope of the claims. Unless a specific order of steps or actions is specified, the order and / or use of specific steps and / or actions may be modified without departing from the scope of the claims.

[0037] Please see Figure 1 This application provides a fluid transport system 100 for transporting fluid to a fluid usage system 1. The fluid usage system 1 is a system for using fluid and is the destination of the fluid transport system 100. In some embodiments, the fluid usage system 1 is a flow cell carrier.

[0038] The fluid transport system 100 includes a fluid transport body 10, and may further include a fluid storage module 20.

[0039] The fluid storage module 20 is used to store multiple fluids that need to be transported to the fluid usage system 1. The fluid storage module 20 includes multiple independent fluid storage units 21, each storing one type of fluid. This fluid may include reagents involved in biochemical reactions, or it may include buffer solutions or cleaning solutions. The fluids stored in the multiple fluid storage units 21 may be different from each other. The multiple fluids are divided into a first category and a second category. The first category fluids are sensitive fluids, meaning that when mixed with at least one other fluid among the multiple fluids, cross-contamination or mutual influence may occur. The second category fluids are non-sensitive fluids, meaning that when mixed with at least one other fluid among the multiple fluids, cross-contamination or mutual influence may not occur. In this application, cross-contamination or mutual influence between two fluids can refer to the fluids themselves producing adverse effects such as mutual repulsion or mutual cancellation when mixed, or it can refer to the two fluids, although not producing adverse effects such as mutual repulsion or mutual cancellation, affecting the normal progress of subsequent biochemical reactions and detection results. In some embodiments, the fluid storage unit 21 is a container suitable for storing and containing fluids, such as a reagent compartment or reagent kit.

[0040] The following uses gene sequencing as an example to illustrate the types of fluids. During gene sequencing, the DNA sample to be tested is first loaded or immobilized into the cavity of a flow cell slide. Subsequently, various functional fluids are systematically and periodically introduced into the cavity of the flow cell slide to achieve a specific function. In some embodiments, the fluids passing through the cavity in a single cycle include four types: synthesis reagent A, buffer B, scanning reagent C, and excision reagent D. Synthesis reagent A contains free bases with fluorescent groups and is used to react with the sample in a synthesis reaction. Buffer B is used to isolate any two of the other three reaction reagents, or to act as a cleaning medium to clean the internal channels of the components through which the reaction reagents flow or the connecting tubing between components. Scanning reagent C is used to increase the stability of the sample when the optical system performs fluorescence imaging of the sample. Excision reagent D is used to remove the fluorescent groups on the sample to facilitate the synthesis reaction in the next cycle. The above cycles introduce reaction reagents into the flow cell slide in the order of "synthesis reagent A - buffer reagent B - scanning reagent C - excision reagent D" until the entire gene sequencing is completed.

[0041] The contact between synthetic reagent A and excision reagent D poses a risk of cross-contamination, affecting the orderly conduct of the aforementioned periodic reactions. For example, if excision reagent D is incorporated into synthetic reagent A during its entry into the chamber, at least some samples will be unable to undergo the synthesis reaction, resulting in no luminescence during the scanning phase and thus incorrect base identification and detection failure for those samples. Similarly, if excision reagent D is incorporated into synthetic reagent A during its entry into the chamber, some samples may come into contact with synthetic reagent A and then immediately with excision reagent D, leading to continuous synthesis and excision reactions and causing detection failure for those samples. Therefore, in this embodiment, synthetic reagent A and excision reagent D are referred to as first-class fluids, and buffer B and scanning reagent C are referred to as second-class fluids.

[0042] The fluid transport body 10 has multiple inlet channels 11 and one confluence channel 13. The multiple inlet channels 11 include a first inlet channel 111 for introducing a first type of fluid and a second inlet channel 112 other than the first inlet channel 111. That is, the second inlet channel 112 is used to introduce a second type of fluid. There can be one or more first inlet channels 111, and one or more second inlet channels 112. A first inlet channel 111 and a second inlet channel 112 converge at a primary confluence point P0, and the multiple inlet channels 11 converge at a secondary confluence point P2, which is located downstream of the primary confluence point P0. The first inlet channel 111 and the second inlet channel 112 form a connecting channel 12 after passing through the primary confluence point P0. Specifically, the secondary confluence point P2 can be the intersection of the connecting channel 12 and the confluence channel 13.

[0043] For ease of description, such as Figure 2 As shown, multiple fluid storage devices 21 are defined, including fluid storage device 21A for storing synthetic reagent A, fluid storage device 21B for storing buffer solution B, fluid storage device 21C for storing scanning reagent C, and fluid storage device 21D for storing resection reagent D. Furthermore, the inlet channel 11 connecting fluid storage device 21A is called inlet channel 11A, the inlet channel 11 connecting fluid storage device 21B is called inlet channel 11B, the inlet channel 11 connecting fluid storage device 21C is called inlet channel 11C, and the inlet channel 11 connecting fluid storage device 21D is called inlet channel 11D. Inlet channels 11A and 11B converge at a primary convergence point P01, and inlet channels 11C and 11D converge at a primary convergence point P02. Inlet channels 11A and 11D constitute the first inlet channel 111, and inlet channels 11B and 11C constitute the second inlet channel 112. For ease of distinction, Figure 2 Differentiate the four fluids—synthetic reagent A, buffer solution B, scanning reagent C, and excision reagent D—by using different filling methods.

[0044] For example, when synthetic reagent A is introduced through inlet channel 11A, it may experience overflow or overshoot at the primary confluence point P01 or the secondary confluence point P2. This overflow phenomenon, verified by both experiments and simulations, refers to the phenomenon where fluid deviates from its intended path and enters another channel at the confluence point due to pressure difference or other reasons. Therefore, in some embodiments, inlet channels 11A and 11B are arranged to converge at the primary confluence point P01, and inlet channels 11C and 11D are arranged to converge at another primary confluence point P02. Since each of the first inlet channels 111 intersects with the second inlet channel 112, the synthetic reagent A formed by overflow will not enter the inlet channel 11D corresponding to another sensitive reagent, i.e., the ablation reagent D, thereby reducing the risk of cross-contamination or mutual influence when the two sensitive reagents come into contact.

[0045] In this application, each of the first inlet channels 111 intersects with a second inlet channel 112, reducing the risk of the first type of fluid flowing into the inlet channel 11 of another first type of fluid. Therefore, when the first type of fluid (such as a sensitive reagent) is introduced into the manifold channel 13 through the first inlet channel 111, if the first type of fluid experiences overflow or overrush at the primary confluence point P0, the fluid formed by the overflow or overrush will exist in the second inlet channel 112, that is, in the channel used to introduce the second type of fluid (such as a non-sensitive reagent). Therefore, when the second type of fluid is subsequently introduced into the manifold channel 13 through the second inlet channel 112, even if the second type of fluid mixes with the fluid formed by the overflow or overrush, there will be no cross-contamination or mutual influence. This also improves the situation where two fluids with a risk of cross-contamination are introduced into the fluid use system 1 at the same time, causing the biochemical reaction to not proceed as expected, which is beneficial to the biochemical reaction and improves the detection accuracy.

[0046] like Figure 1 As shown, in some embodiments, along the fluid flow path within the inlet channel 11, the distance between the primary confluence point P0 and the secondary confluence point P2 is greater than the fluid flow length at the secondary confluence point P2. For example, as Figure 2As shown, when synthetic reagent A is introduced through introduction channel 11A and overflow or overrush occurs at the secondary confluence point P2, since the distance between the primary confluence point P0 and the secondary confluence point P2 is greater than the flow length of the fluid at the secondary confluence point P2, the synthetic reagent A formed by the overflow will not reach the primary confluence point P0, nor will it enter the introduction channel 11D corresponding to the other sensitive reagent, i.e., the ablation reagent D. This further improves or even avoids the risk of cross-contamination or mutual influence when the two sensitive reagents come into contact. It can be understood that the flow length of the fluid may be determined by factors such as the inner diameter of the channel, the pressure inside the channel (or the power of the power module 40), the fluid viscosity, and the fluid velocity. In this embodiment, when designing the distance between the primary confluence point P0 and the secondary confluence point P2 based on the flow length of the fluid, key factors such as the inner diameter of the channel, the fluid viscosity, and the fluid velocity can be input in the simulation to obtain a fixed flow length value. If the result shows that the flow length is greater than the distance between the primary confluence point P0 and the secondary confluence point P2, the distance between the primary confluence point P0 and the secondary confluence point P2 in the model is increased, and the next round of simulation is performed. In this way, the distance value that meets the requirements of this application can be determined through multiple rounds of simulation iteration.

[0047] like Figure 1As shown, in some embodiments, the fluid transport system 100 may further include a valve module 30 and a power module 40. The valve module 30 includes multiple valves 31, and multiple fluid storage units 21 are respectively connected to multiple inlet channels 11 through the multiple valves 31. At least one valve is opened at a time, thereby transferring fluid in the corresponding fluid storage unit 21 to the fluid use system 1 via the corresponding inlet channel 11 and manifold channel 13. The power module 40 can be connected to either the fluid storage unit 21 or the fluid use system 1. The power module 40 is used to create and maintain a pressure gradient (pressure difference) within the fluid transport system 100, thereby driving the fluid to move within the fluid transport system 100. The power module 40 can drive the fluid movement in both positive and negative directions, i.e., positive pressure drive and negative pressure drive. The positive direction refers to the direction that propels the fluid from the fluid storage module 20 sequentially to the fluid transport body 10 and the fluid use system 1, while the negative direction refers to the direction that draws fluid from the fluid storage module 20 and causes it to flow sequentially to the fluid transport body 10 and the fluid use system 1. In some embodiments, the fluid storage unit 21 and the fluid transport body 10 can be connected by a pipeline. The valve 31 can be a control valve on the corresponding pipeline used to control the on / off state, such as a two-way valve or a solenoid valve. The power module 40 can be various types of pumps used to drive fluid movement, such as syringe pumps, plunger pumps, diaphragm pumps, gear pumps, and peristaltic pumps. In this embodiment, the fluid is extracted using a negative pressure drive method. In this case, the power module 40 is connected to the fluid use system 1 and is located downstream of the fluid use system 1. It should be noted that the power module 40 can also be located at other positions in the fluid transport system 100 to meet the requirements of driving fluid movement, such as being located upstream of the fluid transport body 10.

[0048] like Figure 8 As shown, in another embodiment, after the first inlet channel 111 and the second inlet channel 112 form a connecting channel 12 via a primary confluence point P0, the connecting channel 12 also intersects with another first inlet channel 111 or another second inlet channel 112 at a secondary confluence point P1. The secondary confluence point P1 is located downstream of the primary confluence point P0 and upstream of the secondary confluence point P2. While satisfying the rule that every first inlet channel 111 intersects with a second inlet channel 112, by setting more levels of confluence points, the fluid transport system 100 can be used to transport more types of fluids, and the risk of a first type of fluid flowing through the inlet channel 11 of another first type of fluid is reduced, thus improving the problem of cross-contamination. For example, as... Figure 9As shown, the fluid passing through the cavity in a single cycle of gene sequencing includes six types: synthetic reagent A1, synthetic reagent A2, buffer B1, buffer B2, scanning reagent C, and excision reagent D. Synthetic reagent A1, synthetic reagent A2, and excision reagent D are sensitive reagents. Cross-contamination or mutual influence can occur when synthetic reagent A1 and synthetic reagent A2 are mixed with excision reagent D. Therefore, in some embodiments, the introduction channel 11A2 of synthetic reagent A2 and the introduction channel 11B1 of buffer B1 converge at a primary confluence point P01. The introduction channels 11A2 and 11B1 form a connecting channel 12, which intersects with the introduction channel 11A1 of synthetic reagent A1 at a secondary confluence point P11. All introduction channels 11 converge at a secondary confluence point P2. Therefore, synthetic reagents A1 and A2 formed during flow will not enter the introduction channel 11D, thereby reducing the risk of cross-contamination or mutual influence when different sensitive reagents come into contact, especially when the fluid transport system 100 can be used to transport more types of fluids.

[0049] like Figure 8 As shown, in this case, the fluid flow path along the inlet channel 11 can be configured such that the distance between the primary confluence point P0 and the secondary confluence point P1 is greater than the fluid flow length at the secondary confluence point P1, and the distance between the secondary confluence point P1 and the secondary confluence point P2 is greater than the fluid flow length at the secondary confluence point P2. It can be understood that the fluid flow length at the secondary confluence point P1 and the fluid flow length at the secondary confluence point P2 can be the same or different. For example, when the inner diameters of each inlet channel 11, connecting channel, and confluence channel 13 are approximately the same, the fluid flow length at the secondary confluence point P1 and the fluid flow length at the secondary confluence point P2 are approximately the same.

[0050] In some embodiments, the fluid transport body 10 is further provided with a bypass channel 14, which merges with the confluence channel 13 at a tertiary confluence point P3, located downstream of the secondary confluence point P2. The fluid storage module 20 may also include a waste liquid storage device 22 connected to the bypass channel 14. The second type of fluid flowing through the second inlet channel 112 can flow through the bypass channel 14 and then enter the waste liquid storage device 22 for collection. This second type of fluid can introduce the fluid formed during the flow into the waste liquid storage device 22, thereby further improving the cross-contamination problem. In some embodiments, the waste liquid storage device 22 is a container suitable for storing and containing fluids, such as a reagent compartment or reagent kit.

[0051] An embodiment of this application also provides a fluid transport method applied to the above-described fluid transport system 100. The fluid transport method includes the following steps: Step S1, as follows Figure 1 As shown, the above-mentioned fluid transport system 100 is provided.

[0052] Step S2: Connect the multiple inlet channels 11 of the fluid transport body 10 of the fluid transport system 100 to the multiple fluid storage devices 21 respectively, wherein the first inlet channel 111 is connected to the fluid storage device 21 for storing the first type of fluid.

[0053] In this application, "connection" or "connection" refers to a direct connection or connection between modules, or a connection or connection between modules through pipes or other suitable components. For example, in this embodiment, the inlet channel 11 can be connected to the fluid storage device 21 through a pipe.

[0054] In some embodiments, multiple fluid storage devices 21 may be connected to multiple inlet channels 11 via multiple valves 31.

[0055] It is understood that multiple inlet channels 11 are connected to multiple fluid storage devices 21 respectively. This does not mean that the number of fluid storage devices 21 must be exactly the same as the number of inlet channels 11. For example, the number of inlet channels 11 can be more than the number of fluid storage devices 21, and multiple fluid storage devices 21 are connected to a portion of the inlet channels 11 respectively.

[0056] Step S3: Connect the manifold 13 of the fluid transport body 10 to the fluid use system 1.

[0057] In some embodiments, the power module 40 may also be connected to the fluid usage system 1.

[0058] Step S4: Connect multiple fluid storage devices 21 to the manifold channel 13 in sequence, and drive the fluid in the fluid storage devices 21 to be transferred to the fluid use system 1 through the corresponding inlet channel 11.

[0059] In some embodiments, the valve 31 can be controlled to sequentially connect multiple fluid storage units 21 to the manifold 13. Alternatively, the power module 40 can be activated to drive the fluid transfer to the fluid usage system 1. For example, when the fluid passing through the cavity in a single cycle of gene sequencing includes four types of reagents: synthesis reagent A, buffer B, scanning reagent C, and excision reagent D, the corresponding reaction reagents can be periodically introduced into the fluid usage system 1 according to the sequence of "synthesis-scanning-excision". Furthermore, buffer B is introduced into the fluid usage system 1 between the introduction of synthesis reagent A and scanning reagent C, allowing the buffer to isolate the two reaction reagents, or to act as a cleaning medium to clean the internal flow channels of the components through which the reaction reagents flow or the connecting pipes between components.

[0060] The fluid transport method of this application will be further described below with reference to the specific structure of the fluid transport system 100. Those skilled in the art should understand that the structures described in this application are merely embodiments, and any other suitable structures are within the scope of this application.

[0061] Example 1 like Figure 2 As shown, this embodiment illustrates the introduction of four fluids—synthesis reagent A, buffer B, scanning reagent C, and excision reagent D—into the fluid usage system 1 during a single gene sequencing cycle. In each cycle, the reaction reagents are introduced into the flow cell slide in the order of "synthesis reagent A - buffer reagent B - scanning reagent C - excision reagent D - scanning reagent C - buffer B". In this embodiment, introduction channels 11A and 11B converge at a primary confluence point P01, introduction channels 11C and 11D converge at another primary confluence point P02, and all introduction channels 11 converge at a secondary confluence point P2.

[0062] Figure 2 The diagram shows that the synthetic reagent A first flows to the flow cell slide along the inlet channel 11A and the manifold channel 13, and the synthetic reagent A forms a flow at the primary manifold point P01 and the secondary manifold point P2. Therefore, a small amount of synthetic reagent A appears in the inlet channel 11B, and a small amount of synthetic reagent A also appears in the connecting channel 12 between the primary manifold point P02 and the secondary manifold point P2.

[0063] Furthermore, Figure 3 The buffer B flows along the inlet channel 11A and the manifold channel 13 to the flow cell slide, thereby rinsing the synthetic reagent A in the flow cell slide. Buffer B forms a flow at the primary manifold point P01 and the secondary manifold point P2. Therefore, a small amount of buffer B appears in the inlet channel 11A, and a small amount of buffer B also appears in the connecting channel 12 between the primary manifold point P02 and the secondary manifold point P2.

[0064] Furthermore, Figure 4 The scanning reagent C flows along the inlet channel 11C and the manifold channel 13 to the flow cell slide. During this process, the synthetic reagent A and buffer B remaining in the connecting channel 12 from the previous step are introduced into the flow cell slide along with the scanning reagent C. At the same time, the scanning reagent C forms a flow at the primary manifold point P02 and the secondary manifold point P2. Therefore, a small amount of scanning reagent C appears in the inlet channel 11D, and a small amount of scanning reagent C also appears in the connecting channel 12 between the primary manifold point P01 and the secondary manifold point P2.

[0065] Furthermore, Figure 5The diagram shows that the ablation reagent D flows along the inlet channel 11D and the manifold channel 13 to the flow cell slide, and the ablation reagent D forms an overflow at the primary manifold point P02 and the secondary manifold point P2. Therefore, a small amount of ablation reagent D appears in the inlet channel 11C, and a small amount of ablation reagent D also appears in the connecting channel 12 between the primary manifold point P01 and the secondary manifold point P2. However, due to the setting of the primary manifold point P0 and the secondary manifold point P2 in this application, it is possible to improve or even avoid the ablation reagent D formed by the overflow mixing with the synthetic reagent A in the inlet channel 11A and causing cross-contamination.

[0066] In some embodiments, since there is residual excision reagent D in the inlet channel 11C and in the connecting channel 12 between the primary manifold P02 and the secondary manifold P2, in order to remove the residual excision reagent D, scanning reagent C and buffer B are introduced after the excision reagent D is introduced in each cycle.

[0067] Figure 6 The scanning reagent C flows again along the introduction channel 11C and the confluence channel 13 to the flow cell slide, thereby removing the residual excision reagent D in the introduction channel 11C. Figure 7 The buffer B is shown flowing along the inlet channel 11A and the manifold 13 to the flow cell slide, thereby removing residual excision reagent D in the connecting channel 12 between the primary manifold P02 and the secondary manifold P2.

[0068] At this point, a complete liquid cycle is finished, and the two sensitive reagents have not come into contact, thus avoiding cross-contamination. The above process is then repeated periodically, which is understandable because... Figure 7 When buffer B is introduced, overflow occurs at the primary manifold P01 and the secondary manifold P2. Therefore, after the synthetic reagent A is introduced again, the buffer B formed by the above overflow will exist in the connecting channel 12 between the primary manifold P02 and the secondary manifold P2, as follows: Figure 2 As shown.

[0069] Example 2 like Figure 9As shown, this embodiment illustrates the introduction of six fluids—synthetic reagent A1, synthetic reagent A2, buffer B1, buffer B2, scanning reagent C, and excision reagent D—into the fluid usage system 1 during a single gene sequencing cycle. In each cycle, the reaction reagents are introduced into the flow cell slide in the following order: "synthetic reagent A1 - buffer B1 - synthetic reagent A2 - scanning reagent C - buffer B2 - excision reagent D - buffer B2 - buffer B1". Synthetic reagent A1, synthetic reagent A2, and excision reagent D are sensitive reagents, but there is no adverse reaction between synthetic reagent A1 and synthetic reagent A2, and they can be mixed together. In this embodiment, the inlet channel 11A2 of the synthetic reagent A2 and the inlet channel 11B1 of the buffer solution B1 converge at a primary confluence point P01. Inlet channels 11A2 and 11B1 form a connecting channel 12, which intersects with the inlet channel 11A1 of the synthetic reagent A1 at a secondary confluence point P11. The inlet channel 11D of the ablation reagent D and the inlet channel 11B2 of the buffer solution B2 converge at another primary confluence point P02. Inlet channels 11D and 11B2 form a connecting channel 12, which intersects with the inlet channel 11C of the scanning reagent C at another secondary confluence point P12. All inlet channels 11 converge at a secondary confluence point P2. Furthermore, a bypass channel 14 and a confluence channel 13 converge at a tertiary confluence point P3, and the end of the bypass channel 14 facing away from the confluence channel 13 is also connected to the power module 40.

[0070] Figure 9 The diagram shows that the synthetic reagent A1 first flows to the flow cell slide along the inlet channel 11A1 and the manifold channel 13, and the synthetic reagent A1 forms a flow at the junction of the secondary manifold point P11, the secondary manifold point P2 and the bypass channel 14, so that a small amount of synthetic reagent A1 appears in the connecting channel 12 between the primary manifold point P01 and the secondary manifold point P11, the channel between the secondary manifold point P12 and the secondary manifold point P2, and the bypass channel 14. However, due to the setting of the multi-level manifold points in this embodiment, the synthetic reagent A1 does not come into contact with the resection reagent D in the resection channel D and does not cause cross-contamination.

[0071] Furthermore, Figure 10 The buffer solution B1 flows along the inlet channel 11B1 to the bypass channel 14, thereby flushing the synthetic reagent A1 remaining between the primary manifold P01 and the secondary manifold P11 into the bypass channel 14. At the same time, the buffer solution B1 forms a flow at the primary manifold P01, the secondary manifold P11 and the secondary manifold P2. Figure 11 This shows that buffer B1 continues to flow along inlet channel 11B1 and manifold 13 to the flow cell slide, thereby rinsing the synthetic reagent A1 in the flow cell slide.

[0072] Furthermore, Figure 12The diagram shows that the synthetic reagent A2 flows to the flow cell slide along the inlet channel 11A2 and the manifold channel 13. During this process, the synthetic reagent A2 forms a flow at the primary manifold point P01, the secondary manifold point P11, the secondary manifold point P2, and the junction of the bypass channel 14.

[0073] Furthermore, Figure 13 The scanning reagent C flows along the inlet channel 11C to the bypass channel 14, thereby flushing the synthetic reagents A1 and A2 remaining between the secondary manifold P12 and the secondary manifold P2 into the bypass channel 14. Simultaneously, the scanning reagent C creates a flow at the secondary manifold P12 and the secondary manifold P2, resulting in a small amount of scanning reagent C appearing in the connecting channel 12 between the primary manifold P02 and the secondary manifold P12, and in the channel between the secondary manifold P11 and the secondary manifold P2. Figure 14 The scanning reagent C continues to flow along the inlet channel 11C and the confluence channel 13 to the flow cell slide.

[0074] Furthermore, Figure 15 The diagram shows that buffer B2 flows along inlet channel 11B2 to bypass channel 14, thereby flushing the scanning reagent C remaining between the primary manifold P02 and the secondary manifold P12 into bypass channel 14. Simultaneously, buffer B2 creates flow at the primary manifold P02, the secondary manifold P12, and the secondary manifold P2. Figure 16 This shows that buffer B2 continues to flow along inlet channel 11B2 and manifold 13 to the flow cell slide, thereby rinsing the scanning reagent C in the flow cell slide.

[0075] Furthermore, Figure 17 The diagram shows the excision reagent D flowing along the inlet channel 11D and the manifold channel 13 to the flow cell slide, with the excision reagent D forming a flow path at the primary manifold point P02, the secondary manifold point P12, the secondary manifold point P2, and the bypass channel 14. However, due to the multi-stage manifold design of this application, the excision reagent D formed by the flow path does not enter the inlet channels 11A1 and 11A2, thereby avoiding cross-contamination of sensitive reagents.

[0076] Furthermore, Figure 18 The diagram shows buffer B2 flowing along inlet channel 11B2 to bypass channel 14, causing the resection reagent D remaining in inlet channel 11B2 to be flushed into bypass channel 14. Simultaneously, buffer B2 forms overflows at the primary manifold P02, secondary manifold P12, and secondary manifold P2. The resection reagent D that forms overflows between secondary manifold P11 and secondary manifold P2 will be flushed away when scanning reagent C is introduced into bypass channel 14 in the next cycle (see reference). Figure 13 ). Figure 19This shows that buffer B2 continues to flow along inlet channel 11B2 and manifold 13 to the flow cell slide, thereby rinsing the excision reagent D in the flow cell slide.

[0077] Furthermore, Figure 20 This shows that buffer B1 flows along inlet channel 11B1 to bypass channel 14, causing the resection reagent D remaining between secondary manifold P11 and secondary manifold P2 to be flushed into bypass channel 14, thereby allowing the synthesis reagent A1 to be introduced in the next cycle ( Figure 9 (As shown in the diagram) To prevent the synthetic reagent A1 from coming into contact with the excision reagent D and causing cross-contamination, buffer B1 forms overflow at the primary manifold P01, the secondary manifold P11, and the secondary manifold P2. Figure 21 This shows that buffer B1 continues to flow along inlet channel 11B1 and manifold channel 13 to the flow cell slide.

[0078] At this point, a complete liquid cycle is completed, and the three sensitive reagents do not come into contact with each other, thus avoiding cross-contamination. In addition, the three sensitive reagents do not pass through the bypass channel 14 during the cycle; only the non-sensitive reagents (i.e., buffer B1, buffer B2, and scanning reagent D) are introduced into the bypass channel 14. This reduces the risk of cross-contamination between fluids, lowers fluid consumption and cost, and also minimizes the total liquid cycle time.

[0079] An embodiment of this application also provides a method for analyzing biochemical substances, which includes the following steps: Step S1': Perform the above-described fluid transport method so that multiple fluids react biochemically with the sample within the fluid use system 1.

[0080] Step S2': Perform signal detection on the reacted sample to obtain a signal that can represent the biological characteristics of the sample.

[0081] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit it. Although this application has been described in detail with reference to the embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the spirit and scope of the technical solutions of this application.

Claims

1. A fluid transport system for transporting multiple fluids to a fluid use system, characterized in that, The fluid transport system includes: The fluid transport body has multiple inlet channels and a confluence channel. The multiple inlet channels include a first inlet channel for introducing a first type of fluid and a second inlet channel other than the first inlet channel. The first type of fluid is a fluid that will cross-contaminate or easily affect each other when mixed with at least one other fluid among the multiple fluids. The first inlet channel and the second inlet channel converge at a primary inlet point, and the plurality of inlet channels converge at a secondary inlet point, which is located downstream of the primary inlet point.

2. The fluid transport system as described in claim 1, characterized in that, Along the fluid flow path within the inlet channel, the distance between the primary confluence point and the secondary confluence point is greater than the fluid flow length at the second confluence point.

3. The fluid transport system as described in claim 1, characterized in that, The first and second inlet channels form a connecting channel after passing through the primary junction point. The connecting channel also intersects with another first inlet channel or another second inlet channel at a secondary junction point. The secondary junction point is located downstream of the primary junction point and upstream of the secondary junction point.

4. The fluid transport system as described in claim 3, characterized in that, Along the fluid flow path within the inlet channel, the distance between the primary confluence point and the secondary confluence point is greater than the fluid flow length at the secondary confluence point, and the distance between the secondary confluence point and the second-level confluence point is greater than the fluid flow length at the second confluence point.

5. The fluid transport system as described in any one of claims 1 to 4, characterized in that, The fluid transport body is provided with a bypass channel, which merges with the confluence channel at a third-level confluence point, which is located downstream of the second-level confluence point.

6. The fluid transport system as claimed in claim 1, characterized in that, The fluid transport system also includes: Valve module, the valve module comprising multiple valves; A fluid storage module, comprising multiple fluid storage units for storing the various fluids, wherein the multiple fluid storage units are respectively connected to the multiple inlet channels via the multiple valves; and A power module is provided for connecting to the fluid storage device or the fluid use system and for providing power to transfer the fluid from the fluid storage device to the fluid use system.

7. A fluid transport method for transporting multiple fluids to a fluid use system, characterized in that, The fluid transport method includes: Provide a fluid transport system as described in any one of claims 1 to 4; The fluid transport system has multiple inlet channels connected to multiple fluid storage devices. The fluid storage module includes multiple fluid storage devices for storing the multiple fluids. The multiple fluids include a first type of fluid, which is a fluid that will cause cross-contamination or easily affect each other when mixed with at least one other fluid among the multiple fluids. The first inlet channel is connected to the fluid storage device for storing the first type of fluid. Connect the manifold of the fluid transport system to the fluid usage system; The plurality of fluid storage devices are sequentially connected to the manifold channel, and the fluid in the fluid storage devices is driven to be transferred to the fluid use system through the corresponding first or second inlet channel.

8. The fluid transport method as described in claim 7, characterized in that, The fluid transport body is provided with a bypass channel, which merges with the main flow channel at a third-level convergence point. The third-level convergence point is located downstream of the second-level convergence point. The fluid transport method further includes: The bypass channel is connected to the waste liquid storage device, so that the fluid flowing through the second inlet channel is transferred to the waste liquid storage device through the bypass channel.

9. The fluid transport method as described in claim 7, characterized in that, Driving the fluid transfer to the fluid use system includes: Connect the power module to the fluid usage system; and The power module is activated to drive the fluid transfer to the fluid usage system.

10. A method for analyzing biochemical substances, characterized in that, include: Perform the fluid transport method as described in any one of claims 7 to 9, causing the plurality of fluids to undergo a biochemical reaction with the sample within the fluid use system; as well as Signal detection is performed on the reacted sample to obtain a signal that can represent the biological characteristics of the sample.

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