Micro-fluidic chip for preparing high-throughput tumor organoids as well as preparation method and application of micro-fluidic chip

By designing microfluidic chips with top and bottom layer structures, the problems of low throughput, low space utilization, complex recycling, and frequent culture medium updates in existing technologies have been solved, enabling high-throughput, uniform-sized tumor organoid culture and convenient recycling.

CN122344518APending Publication Date: 2026-07-07JINAN UNIVERSITY
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Patent Information

Application Number
CN202510017215.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-06
Publication Date
2026-07-07

AI Technical Summary

Technical Problem

Existing microfluidic chips suffer from problems such as low throughput, low space utilization, complex recycling, non-reusability, and frequent culture medium updates when preparing tumor organoids, making it difficult to achieve high-throughput, uniform-sized tumor sphere culture.

Method used

A microfluidic chip consisting of a top layer and a bottom layer was designed. The top layer has four right-angled fan-shaped hollow areas, and the bottom layer has four cell culture areas and four medium exchange buffers. The cell culture areas contain small chambers, and the medium exchange buffers are connected to the center. The chip is made of polydimethylsiloxane with a hydrophilic polymer coating and a light-transmitting chamber structure, which facilitates observation and recycling.

Benefits of technology

It enables high-throughput, uniform-sized tumor organoid culture, reduces cell interference and loss during medium changes, facilitates uniform seeding of tumor cells and organ recovery, reduces the frequency of medium replacement, and provides higher space utilization and throughput.

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Abstract

The invention discloses a micro-fluidic chip for preparing high-throughput tumor organs as well as a preparation method and application of the micro-fluidic chip. The micro-fluidic chip comprises a top layer and a matched bottom layer connected with the top layer, the top layer comprises four right-angle fan-shaped hollow-out areas which are connected with the bottom layer to form a liquid storage area; four cell culture areas and four liquid change buffer areas are arranged in the bottom layer area corresponding to the top layer hollow area, and one cell culture area and one liquid change buffer area form a right-angle sector; the cell culture area is provided with a plurality of small chambers; the liquid changing buffer area is connected with the circle center. The micro-fluidic chip is provided with the liquid change buffer area, so that the interference and loss to the growth of cells during liquid change are greatly reduced, thereby facilitating the formation of uniform tumor organs, keeping the tumor organs to stay in situ, and facilitating continuous observation; and the structural design with the open top is adopted, so that uniform implantation and dispersion of tumor cells are facilitated, and recovery of tumor organs is facilitated.
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