Competitive quenching oligonucleotide, pea detection kit, dynamic range expansion method and applications
By employing competitive quenching oligonucleotide (CQO) active hybridization inhibition and asymmetric probe concentration design, combined with graded thermal pulse melting technology, the contradiction between cross-interference and sensitivity versus dynamic range in PEA technology was resolved, achieving protein detection with high sensitivity and a wide linear dynamic range.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- MUEN (WUHAN) MEDICAL & BIOTECHNOLOGY CO LTD
- Filing Date
- 2026-04-24
- Publication Date
- 2026-07-17
AI Technical Summary
Existing adjacent extension analysis (PEA) technology faces problems such as severe cross-interference, lag in enzyme alignment mechanism, and contradiction between sensitivity and dynamic range caused by probe concentration design in high-magnification multiplex detection, making it difficult to achieve protein detection with high sensitivity and wide linear dynamic range.
Active hybridization inhibition was achieved by competitive quenching oligonucleotides (CQO), combined with asymmetric probe concentration design and graded thermal pulse melting process. Background noise was reduced and dynamic range was expanded by 3' end extension blocking modification and thiophosphate backbone modification.
It effectively reduces background noise by an order of magnitude, expands the linear dynamic range of the signal response curve by several orders of magnitude, and improves the detection limit, accuracy, and reliability of low-abundance targets.
Smart Images
Figure CN122405809A_ABST