An anti-inflammatory soothing traditional Chinese medicine composition fermentation product and a preparation method and application thereof

Abstract

The application discloses an anti-inflammatory soothing traditional Chinese medicine composition ferment and a preparation method and application thereof, and relates to the field of cosmetics. The traditional Chinese medicine composition ferment is obtained by fermenting traditional Chinese medicine composition forsythia, phellodendron, honeysuckle and dandelion by lactobacillus johnsonii with strain number CICC 6252, significantly improves the content of total flavonoids, chlorogenic acid, berberine and forsythia liposide in the traditional Chinese medicine composition, and obviously enhances the effects of antioxidation, antibiosis, anti-inflammation and soothing, and has a wide application prospect in the fields of cosmetics and medicine. The application also adjusts the mass ratio of the traditional Chinese medicine composition ferment, glycyrrhizic acid dipotassium and allantoin to (3.0-7.0):0.2:0.2, and obtains a composition for anti-inflammation and soothing, and the three components synergistically act to enhance the anti-inflammatory effect.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, specifically to a fermented traditional Chinese medicine composition for anti-inflammatory and soothing purposes, its preparation method, and its application. Background Technology

[0002] Cosmetics, as a traditional skincare product, have functions such as care, repair, and protection for human skin. However, most commercially available cosmetics are formulated with chemically synthesized raw materials and mostly only have the function of moisturizing and hydrating, and cannot repair or care for inflamed, red, swollen, or allergic skin.

[0003] Based on this, CN113967184A provides a compound Phellodendron bark composition with antibacterial and anti-inflammatory properties. This composition achieves its antibacterial and anti-inflammatory effects by adjusting the weight ratio of Forsythia suspensa, Phellodendron bark, Lonicera japonica, and Taraxacum mongolicum. However, this technology uses a traditional water extraction combined with chitosan purification method. Although this method can also obtain certain bioactive components, the extraction rate is very low, which easily leads to the waste of bioactive components.

[0004] Current research indicates that the content of active ingredients in traditional Chinese medicine (TCM) can be increased through microbial fermentation. However, different microorganisms have completely different effects on different TCMs. For example, Aspergillus niger fermentation can increase the flavonoid content in dandelion extract and Phellodendron amurense extract, but it has little effect on the chlorogenic acid content in dandelion extract. On the other hand, fermentation of crude dandelion extract with Bacillus subtilis can increase the chlorogenic acid content in crude dandelion extract. Therefore, when the same strain ferments a TCM composition, the changes in the content of compounds in the fermentation broth are still difficult to predict. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a fermented traditional Chinese medicine composition for anti-inflammatory and soothing purposes, its preparation method, and its applications. The fermented traditional Chinese medicine composition of this invention exhibits significantly increased contents of total flavonoids, chlorogenic acid, berberine, and forsythosides, resulting in significantly enhanced antioxidant, antibacterial, anti-inflammatory, and soothing effects. It has broad application prospects in the cosmetics and pharmaceutical fields. The specific details of this invention are as follows: In a first aspect, the present invention provides a method for preparing a fermentation product of a traditional Chinese medicine composition, the method comprising at least the following steps: S1. Preparation of traditional Chinese medicine composition: Forsythia, Phellodendron bark, honeysuckle and dandelion powders are prepared into powder of traditional Chinese medicine composition in a mass ratio of 2:1:1:1; S2. Preparation of fermentation medium: The fermentation medium comprises 50% to 80% by mass of the traditional Chinese medicine composition powder prepared in S1, 1.5% to 4% by mass of glucose, and 0.5% to 1.0% by mass of yeast extract; S3. Fermentation of microorganisms: The activated strain of Lactobacillus johnsonii, numbered CICC 6252, is inoculated into the fermentation medium obtained in step S2 at an inoculation rate of 2% to 5% to obtain the fermented product of the traditional Chinese medicine composition. The Lactobacillus johnsonii strain numbered CICC 6252 was purchased from the China Industrial Microbial Culture Collection Center.

[0006] Furthermore, the preparation method of Forsythia suspensa, Phellodendron chinense, Lonicera japonica, and Taraxacum mongolicum powders in step S1 includes the following steps: S1-1: Forsythia, Phellodendron bark, honeysuckle and dandelion are pulverized and passed through a 40-mesh sieve to obtain the corresponding Chinese herbal powders.

[0007] Further, the mass fraction of the traditional Chinese medicine composition powder in the fermentation culture medium in step S2 can be 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, or any value between these.

[0008] The mass fraction of glucose in the fermentation medium in step S2 can be 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, or any value between these values.

[0009] The mass fraction of yeast extract in the fermentation medium in step S2 can be 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, 1.0%, or any value between these values.

[0010] Furthermore, in step S3, the fermentation conditions are as follows: place the fermenter in a constant temperature incubator at 35~40℃, allow it to stand or stir at low speed, and ferment for 48-72 hours.

[0011] Furthermore, the method also includes a step of sterilizing and filtering the obtained fermented Chinese medicine composition.

[0012] In a second aspect, the present invention provides a fermented product of a traditional Chinese medicine composition prepared by the method described above.

[0013] In a third aspect, the present invention provides a composition comprising the fermented product of the aforementioned traditional Chinese medicine composition.

[0014] Furthermore, the composition comprises the fermented product of the traditional Chinese medicine composition, dipotassium glycyrrhizate, and allantoin.

[0015] Furthermore, the mass ratio of the fermented Chinese medicine composition, dipotassium glycyrrhizate, and allantoin in the composition is (3.0-7.0):0.2:0.2.

[0016] Furthermore, the composition also includes excipients, matrices, and additives acceptable in the cosmetic and pharmaceutical fields.

[0017] In a fourth aspect, the present invention provides the application of the method or the fermented product of the traditional Chinese medicine composition or the composition in any of the following aspects: A1) Applications in the preparation of anti-inflammatory products; A2) Applications in the preparation of antioxidant products; A3) Applications in the preparation of antibacterial products; A4) Applications in the preparation of anti-aging products; A5) Applications in the preparation of soothing products.

[0018] Furthermore, the product includes one of cosmetics and / or pharmaceuticals.

[0019] Furthermore, the product achieves its antioxidant effect by scavenging free radicals, thereby achieving an anti-aging effect.

[0020] Furthermore, the product achieves a soothing effect through anti-inflammatory and antibacterial properties.

[0021] Furthermore, the drug is an antibacterial or anti-inflammatory drug.

[0022] The beneficial effects of the present invention include, but are not limited to: The fermented traditional Chinese medicine composition of the present invention was obtained by fermenting the traditional Chinese medicine composition of Forsythia suspensa, Phellodendron chinense, Lonicera japonica and Taraxacum mongolicum with Lactobacillus strain number CICC 6252. It significantly increased the content of total flavonoids, chlorogenic acid, berberine and forsythoside in the traditional Chinese medicine composition, and its antioxidant, antibacterial, anti-inflammatory and soothing effects were significantly enhanced. It has broad application prospects in the fields of cosmetics and medicine.

[0023] The present invention also obtained a composition for anti-inflammatory and soothing by adjusting the mass ratio of fermented Chinese medicine composition, dipotassium glycyrrhizate and allantoin to (3.0-7.0):0.2:0.2. The three components work synergistically to further enhance the anti-inflammatory effect. Attached Figure Description

[0024] The accompanying drawings, which are included to provide a further understanding of the invention and form part of this invention, illustrate exemplary embodiments of the invention and are used to explain the invention, but do not constitute an undue limitation of the invention. In the drawings: Figure 1 This is a schematic diagram of the experimental results of DPPH scavenging rate of the fermentation product of the traditional Chinese medicine composition in the embodiments of the present invention; Figure 2 This is a schematic diagram showing the cytotoxicity test results of the fermentation product of the traditional Chinese medicine composition in the embodiments of the present invention. Detailed Implementation

[0025] The present invention is described in detail below with reference to the embodiments, but the present invention is not limited to these embodiments. Unless otherwise specified, the raw materials and catalysts in the embodiments of the present invention are all purchased through commercial channels.

[0026] raw material: Lactobacillus johnsonii: Lactobacillus johnsonii 1: purchased from China Industrial Microbial Culture Collection Center, strain number CICC 10862; Lactobacillus johnsonii 2: purchased from China Industrial Microbial Culture Collection Center, strain number CICC 10861; Lactobacillus johnsonii 3: purchased from China Industrial Microbial Culture Collection Center, strain number CICC 6252.

[0027] MRS culture medium: purchased from Shanghai Crown Biotechnology Co., Ltd.

[0028] Strain activation: Lactobacillus johnsonii: Lactobacillus johnsonii 1-3 were inoculated into MRS medium and cultured anaerobically at 37°C for 24 hours.

[0029] Example 1 1. Raw material processing Forsythia, Phellodendron bark, honeysuckle, and dandelion were pulverized and passed through a 40-mesh sieve to obtain corresponding Chinese herbal powders. The powders of Forsythia, Phellodendron bark, honeysuckle, and dandelion were prepared into Chinese herbal composition 1 in a mass ratio of 2:1:1:1 and added to a culture medium to obtain a fermentation culture medium containing Forsythia, Phellodendron bark, honeysuckle, and dandelion. The mass fractions of various components in the fermentation culture medium were: 50% Chinese herbal composition powder, 2.5% glucose, 0.8% yeast extract, and the remainder was water.

[0030] 2. Strain activation: Lactobacillus johnsonii strains 1-3 were inoculated into MRS liquid medium from slant agar or lyophilized tubes. The culture was incubated statically at 37°C for 18-24 hours, and subcultured twice to obtain a highly active seed culture. Microscopic examination confirmed vigorous bacterial growth and absence of contamination.

[0031] Domestication: The activated strain is inoculated into MRS medium containing 10%-20% of Chinese herbal medicine composition 1, and the proportion of Chinese herbal medicine liquid is gradually increased to adapt it to the fermentation environment.

[0032] 3. Microbial fermentation Under aseptic conditions, the activated *Lactobacillus johnsonii* 1-3 bacterial suspension was inoculated into the cooled fermentation medium (containing 50g of the powder of traditional Chinese medicine composition 1) at an inoculation rate of 3%, and thoroughly mixed. The inoculated medium was placed in a 37℃ constant temperature incubator and allowed to ferment statically or with low-speed stirring. The fermentation time was 72 hours. After fermentation, the fermentation broth was heated in a 75-85℃ water bath for 30 minutes to inactivate the bacteria and terminate the fermentation. The cooled fermentation broth was aliquoted into centrifuge tubes and centrifuged at 6000 rpm for 15-20 minutes. The supernatant was collected to obtain the fermented products of traditional Chinese medicine composition 1-3, which were designated as experimental groups 1-3.

[0033] Comparative Example 1: Forsythia, Phellodendron bark, honeysuckle and dandelion were pulverized and passed through a 40-mesh sieve to obtain the corresponding Chinese herbal powders. The powders of Forsythia, Phellodendron bark, honeysuckle and dandelion were prepared into Chinese herbal composition 1 powder in a mass ratio of 2:1:1:1. 50g of Chinese herbal composition 1 powder was taken and deionized water was added at a material-to-liquid ratio of 1:20. The mixture was extracted in a water bath at about 80℃ for 1 hour. The filtrate was collected by filtration to obtain the Chinese herbal composition extract.

[0034] 4. Content determination and quantitative analysis The contents of total flavonoids (rutin), chlorogenic acid, berberine and forsythoside in the fermentation product of the traditional Chinese medicine composition were detected. The quantitative method is described in Examples 1-3 of the specification of the applicant's invention patent CN110716001A [sections 0063-0111].

[0035] 5. Test Results The content of total flavonoids (rutin), chlorogenic acid, berberine and forsythoside in the extract of the traditional Chinese medicine composition in Comparative Example 1 was recorded as 1, and the relative contents of experimental groups 1-3 were calculated.

[0036] Table 1. Results of determination of total flavonoids (rutin), chlorogenic acid, berberine and forsythoside content

[0037] As shown in Table 1, the berberine content in fermented herbal composition 1 of experimental group 1 was the highest, but the contents of chlorogenic acid and forsythoside were relatively low. The chlorogenic acid content in fermented herbal composition 2 of experimental group 2 did not increase significantly. The total flavonoids, chlorogenic acid, berberine and forsythoside in fermented herbal composition 3 of experimental group 3 were significantly increased. Therefore, fermented herbal composition 3 was selected for subsequent experiments.

[0038] Example 2: Validation of the efficacy of fermented traditional Chinese medicine composition 1. Antioxidant Experiment (1) DPPH removal rate Sample solution preparation: The fermentation product of the traditional Chinese medicine composition 3 and the traditional Chinese medicine composition of Comparative Example 1 were prepared with PBS (pH=7.4) to form a series of concentration gradients (10, 25, 50, 75, 100, 125, 150, 175, 200, 250, 300 μg / mL).

[0039] Preparation of DPPH working solution: Accurately weigh DPPH and prepare a 0.1-0.2 mM working solution with anhydrous methanol. Store in the dark.

[0040] reaction: Take 2.0 mL of sample solution of different concentrations, add 2.0 mL of DPPH working solution, and vortex to mix.

[0041] Blank tube: 2.0 mL PBS (pH=7.4) + 2.0 mL DPPH working solution (to measure initial absorbance A0).

[0042] Sample background tube: 2.0 mL sample solution + 2.0 mL methanol (to eliminate the influence of the sample's own color and determine A1).

[0043] Incubation and measurement: All reaction tubes were placed at room temperature in the dark for 30 minutes. Subsequently, their absorbance values ​​(A2) were measured at a wavelength of 517 nm.

[0044] calculate: DPPH radical scavenging rate (%) = [1 - (A2 - A1) / A0] × 100%.

[0045] Plot the dose-response curve with concentration on the x-axis (X) and clearance rate on the y-axis (Y).

[0046] The half-inhibition concentration (IC50) was calculated using software. 50 The value is the sample concentration required to achieve a clearance rate of 50%.

[0047] The results are as follows Figure 1 As shown, the IC50 of fermented product 3 of the traditional Chinese medicine composition... 50 The concentration was 50 μg / mL, and the IC50 of the control group was... 50 The concentration was 105 μg / mL. This demonstrates that the free radical scavenging ability of the fermented herbal composition 3 is significantly stronger than that of the herbal composition extract in Comparative Example 1.

[0048] 2. Antibacterial test The antibacterial experiment was conducted using the experimental steps outlined in Example 1 of CN113967184A (sections 0059-0067 of the instruction manual). The specific steps are as follows: Experimental samples: the extract of the traditional Chinese medicine composition obtained in Comparative Example 1 of Example 1 of this invention, and the fermentation product 3 of the traditional Chinese medicine composition obtained in Experimental Group 3.

[0049] Staphylococcus aureus CMCC(B)26003 (GDMCC1.221): purchased from Guangdong Huankai Microbial Technology Co., Ltd., product number FSCC(T)223005.

[0050] Streptococcus pneumoniae GDMCC1.2749: purchased from Guangdong Huankai Microbial Technology Co., Ltd. (Huankai), product number FSCC(T)225073.

[0051] Escherichia coli CMCC(B)44102 (GDMCC1.223): purchased from Guangdong Huankai Microbial Technology Co., Ltd., product number FSCC(T)149005.

[0052] Experimental method: The 24-hour slant culture of the test bacteria was washed with PBS to prepare a bacterial suspension (the required concentration was: 100 μL was dropped onto the control sample or into 5 ml of sample solution, and the recovered bacterial count was 1). 10 4 9 10 4 (cfu / mL).

[0053] Take 5 ml each of the experimental sample and the control (water), in 4 tubes for each. Add 100 μL of the bacterial suspension to each sample and control, and mix thoroughly. Start timing and incubate for 2, 5, 10, and 20 minutes. Using sterile forceps, take 0.5 mL of the sample and add it to a test tube containing 5 mL of PBS, mix thoroughly, dilute appropriately, and then take 2... For three dilutions (concentrations of 0.5, 1, and 5 mg / mL), pipette 0.5 mL into two petri dishes and cool to 40°C. Pour 15 mL of nutrient agar medium at 45℃ into the plate, rotate the plate to mix thoroughly, invert the plate after the agar solidifies, and incubate at 35℃±2℃ for 48 h, then count the viable colonies.

[0054] The experiment was repeated 3 times, and the inhibition rate was calculated: X = (A B) / A 100%.

[0055] X represents the inhibition rate (%); A represents the average colony count of the control sample; B represents the average colony count of the test sample.

[0056] Evaluation criteria: Antibacterial rate ≥50%, the product has antibacterial effect; bactericidal rate ≥90%, the product has antibacterial effect (from WS / T 466-2014 "Disinfection Professional Terminology").

[0057] The results are shown in Table 2. As can be seen from Table 2, compared with the extract of the traditional Chinese medicine composition in Comparative Example 1, the fermented product 3 of the traditional Chinese medicine composition significantly increased the antibacterial effect, exhibiting good antibacterial activity at a low concentration (0.5 mg / mL). In particular, the inhibition rate against *Escherichia coli* was significantly increased, presumably because new secretions were generated during fermentation, leading to an increased inhibition rate of *E. coli* by the fermented product 3 of the traditional Chinese medicine composition.

[0058] Table 2 shows the antibacterial test results of fermented product 3 of the traditional Chinese medicine composition.

[0059] 3. Anti-inflammatory experiment In vitro: LPS (lipopolysaccharide) induced RAW 264.7 macrophage inflammation model.

[0060] Cell culture: Mouse mononuclear macrophage leukemia cells (RAW 264.7 cells) were cultured in DMEM high glucose medium (containing 10% FBS and 1% penicillin antibiotics) at 37°C and 5% CO2.

[0061] Cytotoxicity assay (MTT method): Cells were seeded in 96-well plates and cultured for 24 hours until adherence. The medium was then replaced with fresh medium containing different concentrations of fermentation product 3 of the traditional Chinese medicine composition (1, 10, 50, 100, 200 μg / mL). After another 24 hours of culturing, MTT solution was added to each well, and the cells were incubated for 4 hours. The supernatant was discarded, and DMSO was added to dissolve the formazan crystals. The absorbance was measured at 570 nm using a microplate reader. Cell viability was calculated to determine the safe concentration range for subsequent anti-inflammatory experiments (viability >80%). Results are as follows. Figure 2 As shown, by Figure 2 It can be seen that the cell activity of the fermentation product 3 of the traditional Chinese medicine composition is high at concentrations of 1~100 μg / mL.

[0062] Anti-inflammatory activity assay: Cells were divided into the following groups: blank group (complete culture medium), model group (LPS, 1 μg / mL), drug treatment group (LPS + fermentation product 3 of the traditional Chinese medicine composition of experimental group 3 at different concentrations of 10, 25, 50, 100 μg / mL), drug treatment group 5 (100 μg / mL of the traditional Chinese medicine composition extract of comparative example 1), and positive control group 1 (LPS + dexamethasone DEX, 1 μM).

[0063] Except for the control group, all other groups were stimulated with LPS to establish an inflammation model. After co-culturing for 24 hours, the cell supernatant was collected for detection.

[0064] Indicator Testing: NO production determination (Griess method): Take 50 μL of cell supernatant and mix it with an equal volume of Griess reagent (A+B solution) (purchased from Sigma-Aldrich). Incubate at room temperature for 10 min and measure the absorbance at 540 nm. Calculate the NO concentration based on the sodium nitrite standard curve.

[0065] Inflammatory cytokine assay (ELISA method): Using an ELISA kit, strictly following the instructions, the concentrations of TNF-α, IL-6, IL-1β, etc., in the supernatant were measured. The inhibition rates of each inflammatory cytokine were calculated.

[0066] The results are shown in Table 3. It can be seen that the fermentation product 3 of the traditional Chinese medicine composition has a good anti-inflammatory effect when the concentration is 25-100 μg / mL. At the same concentration (100 μg / mL), the anti-inflammatory effect of the fermentation product 3 of the traditional Chinese medicine composition is significantly better than that of the extract of the traditional Chinese medicine composition in Comparative Example 1 (drugated group 5).

[0067] Table 3 shows the anti-inflammatory test results of fermented herbal composition 3.

[0068] Note: This indicates that compared with group 5, P<0.05; This indicates that compared with group 5, P<0.01.

[0069] Example 3: A composition containing fermented Chinese medicine composition 3 (hereinafter referred to as the composition). The fermented product 3 of the traditional Chinese medicine composition was mixed with allantoin and dipotassium glycyrrhizate at a mass ratio of 5.0:0.2:0.2 to obtain a composition containing the fermented product 3 of the traditional Chinese medicine composition. Specifically, the weighed allantoin and dipotassium glycyrrhizate were slowly added to the fermented product 3 of the traditional Chinese medicine composition. The mixture was vigorously shaken with a vortex shaker or thoroughly stirred with a magnetic stirrer to ensure complete and uniform mixing. The mixture was then diluted with deionized water to different concentrations for subsequent experiments.

[0070] Example 4: Efficacy Verification of the Composition 1. Anti-inflammatory experiment The steps are the same as those in the anti-inflammatory experiment in Example 2, Section 3.

[0071] The information for each group is as follows: Treatment group 1: 50 μg / mL of composition 1; Control group 1: 50 μg / mL of fermented traditional Chinese medicine composition 3; Control group 2: 50 μg / mL of allantoin; Control group 3: 50 μg / mL of dipotassium glycyrrhizate. The blank group and model group remained unchanged.

[0072] The results are shown in Table 4. As can be seen from Table 4, dipotassium glycyrrhizate and fermentation product 3 of the traditional Chinese medicine composition synergistically enhance the anti-inflammatory effect, thereby increasing the anti-inflammatory effect.

[0073] Table 4. Anti-inflammatory experimental results of fermentation product 3 of traditional Chinese medicine composition with allantoin and dipotassium glycyrrhizate.

[0074] Note: # indicates that compared with control group 1, P<0.05; # indicates that compared with control group 3, P<0.05; ## indicates that compared with control group 3, P<0.01.

[0075] Example 5: Composition Proportion Optimization The ratios were adjusted to 2:0.2:0.2 (group 2), 3.0:0.2:0.2 (group 3), 7.0:0.2:0.2 (group 4), and 8:0.2:0.2 (group 5), and the steps of Examples 3 and 4 were repeated. The results are shown in Table 5. The results showed that when the ratio of the fermented herbal composition 3 to dipotassium glycyrrhizate and allantoin was 3.0:0.2:0.2 and 7.0:0.2:0.2, the anti-inflammatory effect was significant. However, the effects of the fermented herbal composition 3 to dipotassium glycyrrhizate and allantoin at the ratios of 2.0:0.2:0.2 (group 2) and 8:0.2:0.2 (group 5) were not significantly different from the effect of the fermented herbal composition 3 alone. This demonstrates that when the ratio of the fermented herbal composition 3 to dipotassium glycyrrhizate and allantoin was (3.0~7.0):0.2:0.2, the three components produced a synergistic effect, resulting in the best efficacy.

[0076] Table 5. Anti-inflammatory experimental results of fermented Chinese medicine compositions with different proportions of allantoin and dipotassium glycyrrhizate.

[0077] Note: # indicates that compared with control group 1, P<0.05; # indicates that compared with control group 3, P<0.05; ## indicates that compared with control group 3, P<0.01.

[0078] The above description is merely an embodiment of the present invention, and the scope of protection of the present invention is not limited to these specific embodiments, but is determined by the claims of the present invention. For those skilled in the art, the present invention can have various modifications and variations. Any modifications, equivalent substitutions, improvements, etc., made within the technical concept and principle of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for preparing a fermented product of a traditional Chinese medicine composition, characterized in that, The method includes at least the following steps: S1. Preparation of traditional Chinese medicine composition: Forsythia, Phellodendron bark, honeysuckle and dandelion powders are prepared into powder of traditional Chinese medicine composition in a mass ratio of 2:1:1:1; S2. Preparation of fermentation medium: The fermentation medium comprises 50% to 80% by mass of the traditional Chinese medicine composition powder prepared in S1, 1.5% to 4% by mass of glucose, and 0.5% to 1.0% by mass of yeast extract; S3. Fermentation of microorganisms: The activated strain of Lactobacillus johnsonii, numbered CICC 6252, is inoculated into the fermentation medium obtained in step S2 at an inoculation rate of 2% to 5% to obtain the fermented product of the traditional Chinese medicine composition. The Lactobacillus johnsonii strain numbered CICC 6252 was purchased from the China Industrial Microbial Culture Collection Center.

2. The method according to claim 1, characterized in that, The preparation method of Forsythia suspensa, Phellodendron chinense, Lonicera japonica, and Taraxacum mongolicum powders in step S1 includes the following steps: S1-1: Forsythia, Phellodendron bark, honeysuckle and dandelion are pulverized and passed through a 40-mesh sieve to obtain the corresponding Chinese herbal powders.

3. The method according to claim 1, characterized in that, In step S3, the fermentation conditions are as follows: place the fermenter in a constant temperature incubator at 35~40℃, allow it to stand or stir at low speed, and ferment for 48~72 hours.

4. The method according to claim 1, characterized in that, The method further includes a step of sterilizing and filtering the obtained fermented Chinese medicine composition.

5. Fermented traditional Chinese medicine composition prepared by the method according to any one of claims 1-4.

6. A composition, characterized in that, The composition comprises the fermented traditional Chinese medicine composition of claim 5.

7. A composition, characterized in that, The composition comprises the fermented product of the traditional Chinese medicine composition according to claim 5, dipotassium glycyrrhizate, and allantoin.

8. The composition according to claim 7, characterized in that, The mass ratio of the fermented Chinese herbal medicine composition, dipotassium glycyrrhizate, and allantoin in the composition is (3.0-7.0):0.2:0.

2.

9. The use of the method according to any one of claims 1-4, the fermented product of the traditional Chinese medicine composition according to claim 5, or the composition according to any one of claims 6-8 in any of the following aspects: A1) Applications in the preparation of anti-inflammatory products; A2) Applications in the preparation of antioxidant products; A3) Applications in the preparation of antibacterial products; A4) Applications in the preparation of anti-aging products; A5) Applications in the preparation of soothing products.

10. The application according to claim 9, characterized in that, The product includes at least one of cosmetics and / or pharmaceuticals.

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