A composition for eliminating acne and smoothing the skin and a method for preparing the same

Abstract

The application relates to the technical field of medicines or cosmetics, and particularly discloses a composition for eliminating acne and smoothing skin and a preparation method thereof, which is prepared from components including a prebiotic combination, a plant extract, a fermentation extract, a bioactive ingredient and a composite probiotic freeze-dried powder; the prebiotic combination contains raw materials of galactooligosaccharide and isomaltooligosaccharide; the plant extract contains raw materials of cherry powder; the fermentation extract is prepared from spilanthes acmella, houttuynia cordata and lily after probiotic fermentation; the bioactive ingredient contains raw materials of yeast zinc and yeast beta-glucan; the composite probiotic freeze-dried powder contains a probiotic powder composed of paracasei LY12, lactobacillus plantarum LP28 and lactobacillus rhamnosus LRH113, lactobacillus acidophilus LA1 and lactobacillus rhamnosus GG. The application solves the problems of poor acne treatment effect, easy recurrence and large side effect in the prior art, and finally realizes the effect of efficiently eliminating acne and smoothing skin.

Description

Technical Field

[0001] This application relates to the field of pharmaceutical or cosmetic technology, and more specifically, it relates to a composition for eliminating acne and smoothing the skin, and a method for preparing the same. Background Technology

[0002] Acne is a common chronic inflammatory skin disease that primarily affects the pilosebaceous unit. It is most prevalent in adolescents, with an incidence rate as high as 85%, and also affects approximately 20% of adults. Its pathogenesis is complex, mainly related to elevated androgen levels leading to hypersecretion of sebaceous glands, abnormal keratinization of the pilosebaceous duct, excessive proliferation of Propionibacterium acnes, and the resulting inflammatory response. In addition, genetic factors, dietary habits, irregular lifestyle, mental stress, and improper use of cosmetics can all induce or aggravate acne. Acne not only affects the patient's facial appearance but also often leads to psychological problems such as anxiety and low self-esteem; in severe cases, it can even leave permanent scars.

[0003] Existing treatment options mainly include: topical medications, such as retinoids and benzoyl peroxide, aimed at inhibiting sebum secretion, exfoliating, or killing bacteria; oral medications, including antibiotics, isotretinoin, and hormonal drugs, used to control severe acne; and physical therapies, such as photodynamic therapy, used as adjunctive treatment and to prevent scarring. However, existing technologies generally have limitations: long-term use of antibiotics can easily lead to bacterial resistance and damage the original skin and intestinal flora barrier, weakening local immunity and creating conditions for pathogens to invade again, causing secondary infections or endocrine disorders; hormonal drugs are addictive and all come with varying degrees of toxic side effects, with long-term use accumulating risks; in addition, these methods mostly focus on "sterilization," "oil control," or "exfoliation," failing to address the root cause of the problem by regulating the body's immune function and restoring the balance of the microecology, resulting in a high relapse rate after discontinuation of medication.

[0004] Therefore, there is an urgent need to propose a solution to address the aforementioned technical problems. Summary of the Invention

[0005] In order to address the problems of poor treatment effect, easy recurrence and large side effects of acne in the prior art, and to achieve the goal of eliminating acne from the inside out and smoothing the skin, this application provides a composition for eliminating acne and smoothing the skin and a method for preparing the same.

[0006] In a first aspect, this application provides a composition for eliminating acne and smoothing the skin, employing the following technical solution: A composition for eliminating acne and smoothing the skin, comprising a prebiotic blend, plant extracts, fermented extracts, bioactive ingredients and a compound probiotic freeze-dried powder; The prebiotic composition contains raw materials of galactooligosaccharides and isomaltooligosaccharides; The plant extract contains cherry powder as a raw material; The fermented extract was prepared by fermenting purslane, houttuynia cordata, and lily with probiotics. The bioactive components include raw materials for yeast zinc and yeast β-glucan; The compound probiotic freeze-dried powder contains a probiotic powder composed of Lactobacillus paracasei LY12, Lactobacillus plantarum LP28 and Lactobacillus rhamnosus LRH113, Lactobacillus acidophilus LA1 and Lactobacillus rhamnosus GG. The ingredients, by weight, include 0.1-2 parts galactooligosaccharide, 0.1-2 parts isomaltooligosaccharide, 0.1-2 parts cherry powder, 0.1-0.6 parts fermentation extract, 0.1-2 parts yeast zinc, 0.1-2 parts yeast β-glucan, and 0.2-0.5 parts compound probiotic freeze-dried powder. Additionally, each gram of the composition for eliminating acne and smoothing the skin contains: Lactobacillus paracasei LY12 1×10 9 -6×10 9 1×10 probiotic powder composed of CFU, Lactobacillus plantarum LP28 and Lactobacillus rhamnosus LRH113 9 -4×10 9 CFU, Lactobacillus acidophilus LA1 5×10 8 -3×10 9 CFU and Lactobacillus rhamnosus GG 5×10 8 -3×10 9 CFU.

[0007] By adopting the above technical solutions, galactooligosaccharides (GOS) are a low-molecular-weight, water-soluble dietary fiber. They are the only oligosaccharides that can be utilized by eight major probiotics in the human gut: Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium adolescentis, Lactobacillus acidophilus, Lactobacillus casei, and Lactobacillus salivarius. Harmful bacteria can hardly utilize them. Isomaltooligosaccharides (ISOs) are indigestible sugars, not easily digested and absorbed by the human stomach and small intestine. They directly enter the large intestine and are preferentially utilized by beneficial bacteria such as Bifidobacterium, while harmful bacteria such as Escherichia coli and Salmonella cannot utilize them, thus promoting a positive adjustment of the intestinal microbiota. Combining GOS and ISOs as a prebiotic combination can selectively promote the proliferation of beneficial intestinal bacteria, regulate the intestinal microecological environment, indirectly regulate the body's immune function, and promote the digestion and absorption of vitamins and minerals.

[0008] Cherry powder is rich in nutrients such as protein, sugar, phosphorus, carotene, and vitamin C, and is especially high in iron. It has the effects of moisturizing the skin, eliminating acne scars, promoting skin repair, and reducing the formation of acne marks.

[0009] The fermented extract is made from purslane, houttuynia cordata, and lily through probiotic fermentation. After fermentation by probiotics, the active ingredients are released and transformed, and the bioavailability is significantly improved. It has a significant effect on eliminating pustular, nodular, and conglobate acne.

[0010] Yeast zinc belongs to the third generation of bioavailable zinc. It can enhance the body's immune function, promote normal skin metabolism, enable normal differentiation of epithelial cells, reduce keratinization of the hair follicle sebaceous gland duct opening, and reduce sebum secretion, thereby achieving the effect of preventing and alleviating acne. Yeast β-glucan is the first glucan discovered to have immune activity. It has a unique targeting effect and can directionally remove toxins from the body, enabling the human immune system to quickly reach optimal balance. The combination of these two bioactive ingredients can synergistically assist in the treatment of acne from two dimensions: immune regulation and metabolic regulation.

[0011] The compound probiotic freeze-dried powder, through the synergistic effect of five strains, can not only decompose excess oil on the skin surface and reduce pore blockage through the enzymes produced by metabolism, but also inhibit the excessive growth of pathogenic microorganisms such as Propionibacterium acnes, maintain the skin's microbial balance, soften and dissolve keratin plugs in hair follicles, allowing excess sebum to be discharged normally, and also eliminate bacteria and pathogens that cause skin inflammation, thus eliminating inflammatory reactions.

[0012] By combining the aforementioned prebiotic blends, plant extracts, fermented extracts, bioactive ingredients, and compound probiotic freeze-dried powder, not only is the synergistic effect of "treating bacteria with bacteria" and "internal regulation and external nourishment" achieved, but it also breaks through the traditional "sterilization" mode, rebuilds the microecological barrier, and establishes a multi-mechanism of regulating immunity, controlling oil and reducing inflammation, and repairing the skin, thus treating both the symptoms and the root cause. This achieves the comprehensive goal of regulating the endocrine and immune systems endogenously and repairing the skin's microecological barrier exogenously. It also solves the problems of poor acne treatment effect, easy recurrence, and large side effects in existing technologies, ultimately achieving the effect of effectively eliminating acne and smoothing the skin.

[0013] Preferably, in the probiotic powder composed of *Lactobacillus plantarum* LP28 and *Lactobacillus rhamnosus* LRH113, the ratio of live bacteria of *Lactobacillus plantarum* LP28 to *Lactobacillus rhamnosus* LRH113 is 1:1.

[0014] By adopting the above technical solutions, the two components respectively target systemic anti-inflammatory / antibacterial (LP28) and barrier repair / immune tolerance (LRH113). The compound probiotic powder in this ratio achieves optimal synergy in four dimensions: antibacterial colonization, inflammatory balance regulation, barrier repair, and synergy with prebiotics. Together with other components, it achieves "eliminating acne and smoothing skin" and reduces the recurrence rate. It overcomes the shortcomings of efficacy caused by single strains or arbitrary ratios and exerts a superior effect.

[0015] Preferably, the fermentation extract is prepared by the following operation: S1. Take purslane, houttuynia cordata and lily raw materials, crush and mix them, add pure water, heat to 80-100℃ and decoct for 1-2 hours, filter to obtain the first extract; then add pure water to the obtained filter residue, decoct at 80-100℃ for 0.5-1 hours, filter to obtain the second extract; combine the first extract and the second extract and concentrate to obtain the concentrated solution; S2. Inoculate the activated probiotic seed liquid into the concentrate and anaerobic ferment at 37℃ for 40-50h to obtain the fermentation product. After sterilizing the fermentation product, centrifuge to obtain the supernatant, and then concentrate and spray dry under reduced pressure to obtain the fermentation extract.

[0016] By adopting the above technical solution, in step S1, the anti-inflammatory, antibacterial, and moisturizing active ingredients in the three medicinal materials are maximized through two gradient decoctions, water extraction, and appropriate concentration, and a high-quality natural carbon and nitrogen source culture medium is provided for probiotics. Next, in step S2, the flavonoids in purslane are hydrolyzed into quercetin by probiotic β-glucosidase. The aglycone has a smaller molecular weight, better transdermal absorption, and significantly higher anti-inflammatory activity than the original glycoside. Lily polysaccharides are partially degraded into oligosaccharides / monosaccharides. Oligosaccharides can be used as prebiotics by skin symbiotic bacteria, and their permeability is enhanced after the molecular weight is reduced. The stability of decanoyl acetaldehyde in houttuynia cordata is improved during fermentation. At the same time, the probiotics themselves produce lactic acid and acetic acid, which can inhibit the proliferation of Propionibacterium acnes. The bacteriocins and extracellular polysaccharides (EPS) produced by probiotic metabolism undergo Maillard / esterification reactions with plant components, directly inhibiting and killing Propionibacterium acnes, and achieving immune regulation and assisting in the repair of the skin barrier. In this way, the original anti-inflammatory and antibacterial components in the three medicinal materials of purslane, houttuynia cordata, and lily are deeply explored and upgraded. The fermented extract prepared by the above method can play a triple role in application: "highly effective anti-inflammatory + mild antibacterial + barrier repair". It can also form a highly effective and deep complement to prebiotics, probiotics and yeast zinc / β-glucan, and jointly solve the problems of poor treatment effect, easy recurrence and large side effects of existing technologies for acne treatment, so as to achieve the goal of eliminating acne and smoothing the skin from the inside out.

[0017] Preferably, the compound probiotic freeze-dried powder is obtained by mixing probiotic powder composed of Lactobacillus paracasei LY12, Lactobacillus plantarum LP28 and Lactobacillus rhamnosus LRH113, Lactobacillus acidophilus LA1 and Lactobacillus rhamnosus GG in a certain proportion, adding a protectant, resuspending and freeze-drying; the protectant contains skim milk powder, trehalose and monosodium glutamate, and the weight ratio of skim milk powder, trehalose and monosodium glutamate is (2.5-3.5):(1.5-2.5):1.

[0018] By adopting the above technical solution, the use of protective agents includes skim milk powder providing the main structural framework and volume filling, ensuring good formability, loose and porous structure of the freeze-dried block, and easy reconstitution; trehalose keeping the freeze-dried powder in a glassy state at higher temperatures, delaying the decay of live bacteria; and monosodium glutamate primarily buffering pH and acting as an antioxidant. The synergistic effect of these three agents ensures high viable counts, accurate proportions, and storage stability of multiple strains of bacteria in the compound probiotic freeze-dried powder. While the protective agent does not directly participate in treatment processes such as "sterilization, oil control, and anti-inflammation," it plays a fundamental and prerequisite supporting role by ensuring a continuous supply of live bacterial metabolites, assisting in moisturizing, anti-inflammation, and fading acne scars, and by ensuring the activity of probiotics. This ultimately achieves the core objective of "eliminating acne, smoothing skin, and reducing recurrence."

[0019] Preferably, the prebiotic composition further includes 0.5-1.5 parts by weight of efficacy adjuvant A, which is composed of inulin and arabinoxylan, and the weight ratio of inulin to arabinoxylan is (1.8-2.5):1.

[0020] By adopting the above technical solution, and adding efficacy adjuvant A, which is a compound of inulin and arabinoxylan in a weight ratio of (1.8-2.5):1, to the prebiotic combination, a four-dimensional prebiotic matrix of "rapid start-up - mid-term maintenance - distal coverage + broadening of gut microbiota" is constructed in synergy with the original galactooligosaccharides and isomaltooligosaccharides. Inulin undergoes slow and continuous fermentation in the cecum and proximal colon, selectively promoting the proliferation of bifidobacteria, maintaining the stable release of short-chain fatty acids (especially butyric acid and propionic acid), and inhibiting sebum synthesis and systemic inflammation from the upstream through the gut-epidermal axis. Arabinoxylan undergoes segmental fermentation throughout the colon, broadening the spectrum of stimulated probiotics and further consolidating the dominance of bifidobacteria through cross-feeding effects, enhancing the robustness of the gut microbiota network. The combination of the two effectively prolongs the duration of the microecological regulatory effect, enabling the gut microbiota to maintain a homeostasis of "high beneficial bacteria and low inflammation" after drug withdrawal, significantly reducing the acne recurrence rate, and effectively improving the effect of eliminating acne and smoothing the skin. Meanwhile, all of the functional adjuvant A are natural dietary fiber components, with no drug resistance, no liver or kidney toxicity, and no risk of hormone dependence, making them safe and reliable.

[0021] Preferably, the weight ratio of inulin to arabinoxylan is 2:1.

[0022] By adopting the above technical solution, the combined effects of inulin and arabinoxylan are better, resulting in a composition with better application quality for eliminating acne and smoothing the skin.

[0023] Preferably, the bioactive ingredient further includes 0.3-0.4 parts by weight of efficacy adjuvant B, which is composed of astaxanthin and nicotinamide riboside, and the weight ratio of astaxanthin to nicotinamide riboside is 1:(5-7).

[0024] By employing the above technical solution, an efficacy adjuvant B, composed of astaxanthin and nicotinamide riboside in a weight ratio of 1:(5-7), is added to the bioactive components. This adjuvant works synergistically with the existing yeast zinc and yeast β-glucan to construct a four-in-one system of action: "oil control, immune regulation, cellular energy repair, and antioxidant defense." Astaxanthin, as a potent fat-soluble antioxidant, embeds itself in the cell membrane bilayer structure, efficiently quenching reactive oxygen species, blocking inflammatory signaling pathways, and protecting mitochondrial function; nicotinamide riboside, as an NAD+... + The precursor, after being converted into NMN by the NRK1 enzyme, increases intracellular NAD. + The pool activates Sirt1 / Sirt3, promoting mitochondrial oxidative phosphorylation repair in sebaceous gland cells, upregulating the expression of keratinocyte barrier-related proteins (figrin, stigmacin), and synergistically inhibiting NF-κB transcription through Sirt1. The combination of these two components forms a dual protective mechanism of "antioxidant protection + energy metabolism reconstruction," clearing high levels of reactive oxygen species in acne lesions and preventing NAD+ damage. + On the one hand, it is over-consumed, and on the other hand, it directly replenishes NAD. + The precursor repairs cellular energy metabolism, restoring damaged sebaceous gland cells to normal differentiation and lipid metabolism, thus correcting deep-seated abnormalities in acne pathogenesis at the cellular metabolic level. Simultaneously, astaxanthin inhibits melanocyte tyrosinase activity, and nicotinamide riboside promotes the synthesis of type I and type III collagen. These two components synergistically accelerate the fading of post-inflammatory hyperpigmentation and dermal matrix remodeling, significantly improving post-healing skin texture. Therefore, the use of efficacy adjuvant B can effectively enhance acne treatment by eliminating acne from the inside out, resulting in smooth skin, low recurrence, and high safety.

[0025] Preferably, the weight ratio of astaxanthin to nicotinamide riboside is 1:6.

[0026] By adopting the above technical solution, the combined effects of astaxanthin and nicotinamide riboside are better, resulting in a composition with better application quality for eliminating acne and smoothing the skin.

[0027] Secondly, this application provides a method for preparing a composition for eliminating acne and smoothing the skin, employing the following technical solution: A method for preparing a composition for eliminating acne and smoothing the skin includes the following steps: (1) Prepare raw materials containing prebiotic combination, plant extract, fermentation extract, bioactive ingredients and compound probiotic freeze-dried powder according to the formula; (2) After mixing the prebiotic combination, plant extract, fermentation extract and bioactive ingredients in step (1) evenly, add the compound probiotic freeze-dried powder and mix to obtain a composition for eliminating acne and smoothing the skin.

[0028] Thirdly, this application provides the use of a composition for eliminating acne and smoothing skin in the preparation of pharmaceuticals, health products or cosmetics for eliminating acne and smoothing skin.

[0029] In summary, this application has the following beneficial effects: 1. This application, through the synergistic combination of a prebiotic combination containing galactooligosaccharides and isomaltooligosaccharides, a plant extract containing cherry powder, a fermented extract obtained from purslane-houttuynia cordata-lily through probiotic fermentation, bioactive components containing yeast zinc and yeast β-glucan, and a probiotic powder composed of Lactobacillus paracasei LY12, Lactobacillus plantarum LP28 and Lactobacillus rhamnosus LRH113, and a compound probiotic freeze-dried powder of Lactobacillus acidophilus LA1 and Lactobacillus rhamnosus GG, can effectively solve the problems of poor acne treatment effect, easy recurrence and large side effects in the prior art, and ultimately achieve the effect of effectively eliminating acne and smoothing the skin.

[0030] 2. This application adds efficacy adjuvant A, which is a compound of inulin and arabinoxylan in a weight ratio of (1.8-2.5):1, to the prebiotic combination, and efficacy adjuvant B, which is a compound of astaxanthin and nicotinamide riboside in a weight ratio of 1:(5-7), to the bioactive ingredients. Both of these can effectively enhance the effects of "eliminating acne from the inside out, smoothing the skin, reducing recurrence, and increasing safety", ultimately resulting in a composition with better application quality for eliminating acne and smoothing the skin. Detailed Implementation

[0031] The present application will be further described in detail below with reference to preparation examples, embodiments and comparative examples.

[0032] Unless otherwise specified, all raw materials used in the preparation examples, embodiments and comparative examples of this application are commercially available.

[0033] The cherry powder was purchased from Jiangsu Caiwei Biotechnology Co., Ltd., and is food grade. Inulin was purchased from Guangdong Mingtong Biotechnology Co., Ltd., and is food grade. The arabinoxylan was purchased from Hebei Kelongduo Biotechnology Co., Ltd., and is food grade.

[0034] Preparation examples of raw materials and / or intermediates Preparation Example 1 A fermentation extract is prepared by the following steps: S1. Take dried purslane, houttuynia cordata, and lily bulb raw materials, crush them to 40 mesh in a mass ratio of 1:1:1, mix them, add pure water, the material-to-liquid ratio is 1:15 (w / v), heat to 90℃ and decoct for 1.5 hours, filter to obtain the first extract; then add pure water to the obtained filter residue, the material-to-liquid ratio is 1:10 (w / v), decoct at 90℃ for 0.75 hours, filter to obtain the second extract; combine the first extract and the second extract and concentrate to one-third of the original volume to obtain the concentrated liquid; S2. Inoculate the concentrated liquid with activated Lactobacillus plantarum LP28 probiotic seed liquid (inoculation amount is 5% of the volume of concentrated liquid), and anaerobic ferment at 37℃ for 45h to obtain fermentation product; sterilize the fermentation product at 75℃ for 20min, then centrifuge to obtain the supernatant, then concentrate under reduced pressure to one-fifth of the original volume and spray dry to obtain fermentation extract.

[0035] Preparation Example 2 A fermentation extract, which differs from Preparation Example 1, is prepared by the following operation: S1. Take dried purslane, houttuynia cordata, and lily bulb raw materials, grind them to 40 mesh in a mass ratio of 1:1:1, mix them, add pure water, the material-to-liquid ratio is 1:15 (w / v), heat to 80℃ and decoct for 2 hours, filter to obtain the first extract; then add pure water to the obtained filter residue, the material-to-liquid ratio is 1:10 (w / v), decoct at 80℃ for 1 hour, filter to obtain the second extract; combine the first extract and the second extract and concentrate to one-third of the original volume to obtain the concentrated liquid; S2. Inoculate the concentrated liquid with activated Lactobacillus plantarum LP28 probiotic seed liquid (inoculation amount is 5% of the volume of concentrated liquid), and anaerobic ferment at 37℃ for 50h to obtain fermentation product; sterilize the fermentation product at 75℃ for 20min, then centrifuge to obtain the supernatant, then concentrate under reduced pressure to one-fifth of the original volume and spray dry to obtain fermentation extract.

[0036] Preparation Example 3 A fermentation extract, which differs from Preparation Example 1, is prepared by the following operation: S1. Take dried purslane, houttuynia cordata, and lily bulb raw materials, grind them to 40 mesh in a mass ratio of 1:1:1, mix them, add pure water, the material-to-liquid ratio is 1:15 (w / v), heat to 100℃ and decoct for 1 hour, filter to obtain the first extract; then add pure water to the obtained filter residue, the material-to-liquid ratio is 1:10 (w / v), decoct at 100℃ for 0.5 hours, filter to obtain the second extract; combine the first extract and the second extract and concentrate to one-third of the original volume to obtain the concentrated liquid; S2. Inoculate the concentrated liquid with activated Lactobacillus plantarum LP28 probiotic seed liquid (inoculation amount is 5% of the volume of concentrated liquid), and anaerobic ferment at 37℃ for 40h to obtain fermentation product; sterilize the fermentation product at 75℃ for 20min, then centrifuge to obtain the supernatant, then concentrate under reduced pressure to one-fifth of the original volume and spray dry to obtain fermentation extract.

[0037] Example Example 1

[0038] A composition for eliminating acne and smoothing the skin is made from components comprising a prebiotic blend, plant extracts, fermented extracts, bioactive ingredients, and a compound probiotic freeze-dried powder; the raw materials and their corresponding weight parts are shown in Table 1, and it is prepared by the following steps: (1) Prepare raw materials containing prebiotic combination, plant extract, fermentation extract, bioactive ingredients and compound probiotic freeze-dried powder according to the formula; (2) After mixing the prebiotic combination, plant extract, fermentation extract and bioactive ingredients in step (1) evenly, add the compound probiotic freeze-dried powder and mix to obtain a composition for eliminating acne and smoothing the skin.

[0039] Note: In the above operations, the prebiotic composition consists of galactooligosaccharides and isomaltooligosaccharides; the plant extract is cherry powder; the fermented extract is obtained from purslane, houttuynia cordata, and lily through probiotic fermentation, and is obtained from Preparation Example 1; the bioactive components consist of yeast zinc and yeast β-glucan; the compound probiotic freeze-dried powder contains probiotic powder composed of *Lactobacillus paracasei* LY12, *Lactobacillus plantarum* LP28, and *Lactobacillus rhamnosus* LRH113, *Lactobacillus acidophilus* LA1, and *Lactobacillus rhamnosus* GG, wherein the live bacteria ratio of *Lactobacillus plantarum* LP28 and *Lactobacillus rhamnosus* LRH113 is 1:1, and each gram of the composition for eliminating acne and smoothing the skin contains: *Lactobacillus paracasei* LY12 3.5 × 10 9 Probiotic powder composed of CFU, Lactobacillus plantarum LP28, and Lactobacillus rhamnosus LRH113 2.5×10 9 CFU, Lactobacillus acidophilus LA1 1.75×10 9 CFU and Lactobacillus rhamnosus GG 1.75×10 9 CFU.

[0040] Meanwhile, the compound probiotic freeze-dried powder is obtained by mixing probiotic powder composed of Lactobacillus paracasei LY12, Lactobacillus plantarum LP28 and Lactobacillus rhamnosus LRH113, Lactobacillus acidophilus LA1 and Lactobacillus rhamnosus GG in a certain proportion, adding a protectant, resuspending and freeze-drying; the protectant contains skim milk powder, trehalose and monosodium glutamate, and the weight ratio of skim milk powder, trehalose and monosodium glutamate is 3:2:1.

[0041] Example 2-3

[0042] A composition for eliminating acne and smoothing the skin, which differs from Example 1 in that the raw materials and corresponding weight parts are shown in Table 1.

[0043] Table 1. Raw materials and corresponding weight parts (parts / kg) for the preparation of Examples 1-3

[0044] Example 4

[0045] A composition for eliminating acne and smoothing the skin, which differs from Example 1 in that the fermented extract is obtained from Preparation Example 2.

[0046] Example 5

[0047] A composition for eliminating acne and smoothing the skin, which differs from Example 1 in that the fermented extract is obtained from Preparation Example 3.

[0048] Example 6

[0049] A composition for eliminating acne and smoothing the skin, differing from Example 1 in that each gram of the composition for eliminating acne and smoothing the skin contains: Lactobacillus paracasei LY12 1×10 9 1×10 probiotic powder composed of CFU, Lactobacillus plantarum LP28 and Lactobacillus rhamnosus LRH113 9 CFU, Lactobacillus acidophilus LA1 5×10 8 CFU and Lactobacillus rhamnosus GG 5×10 8 CFU.

[0050] Example 7

[0051] A composition for eliminating acne and smoothing the skin, differing from Example 1 in that each gram of the composition for eliminating acne and smoothing the skin contains: Lactobacillus paracasei LY12 6 × 10 9 4×10g of probiotic powder composed of CFU, Lactobacillus plantarum LP28 and Lactobacillus rhamnosus LRH113 9 CFU, Lactobacillus acidophilus LA1 3×10 9CFU and Lactobacillus rhamnosus GG 3×10 9 CFU.

[0052] Example 8

[0053] A composition for eliminating acne and smoothing the skin, differing from Example 1 in that the weight ratio of skim milk powder, trehalose, and monosodium glutamate is 2.5:1.5:1.

[0054] Example 9

[0055] A composition for eliminating acne and smoothing the skin, differing from Example 1 in that the weight ratio of skim milk powder, trehalose, and monosodium glutamate is 3.5:2.5:1.

[0056] Example 10

[0057] A composition for eliminating acne and smoothing the skin, differing from Example 1 in that the prebiotic composition also contains 1 part by weight of efficacy adjuvant A, which is composed of inulin and arabinoxylan in a weight ratio of 2:1.

[0058] Example 11

[0059] A composition for eliminating acne and smoothing the skin, which differs from Example 10 in that the efficacy adjuvant A is added in 0.5 parts by weight.

[0060] Example 12

[0061] A composition for eliminating acne and smoothing the skin, which differs from Example 10 in that the efficacy adjuvant A is added in 1.5 parts by weight.

[0062] Example 13

[0063] A composition for eliminating acne and smoothing the skin, differing from Example 10 in that the weight ratio of inulin to arabinoxylan is 1.8:1.

[0064] Example 14

[0065] A composition for eliminating acne and smoothing the skin, differing from Example 10 in that the weight ratio of inulin to arabinoxylan is 2.5:1.

[0066] Example 15

[0067] A composition for eliminating acne and smoothing the skin, differing from Example 10 in that the weight ratio of inulin to arabinoxylan is 2.15:1.

[0068] Example 16

[0069] A composition for eliminating acne and smoothing the skin, which differs from Example 10 in that inulin is not used in the prebiotic composition.

[0070] Example 17

[0071] A composition for eliminating acne and smoothing the skin, which differs from Example 10 in that arabinoxylan is not used in the prebiotic composition.

[0072] Example 18

[0073] A composition for eliminating acne and smoothing the skin, differing from Example 1 in that the bioactive ingredients also include 0.35 parts by weight of efficacy adjuvant B, which is composed of astaxanthin and nicotinamide riboside in a weight ratio of 1:6.

[0074] Example 19

[0075] A composition for eliminating acne and smoothing the skin, differing from Example 18 in that the efficacy adjuvant B is added in 0.3 parts by weight.

[0076] Example 20

[0077] A composition for eliminating acne and smoothing the skin, differing from Example 18 in that the efficacy adjuvant B is added in 0.4 parts by weight.

[0078] Example 21

[0079] A composition for eliminating acne and smoothing the skin, differing from Example 18 in that the weight ratio of astaxanthin and nicotinamide riboside is 1:5.

[0080] Example 22

[0081] A composition for eliminating acne and smoothing the skin, differing from Example 18 in that the weight ratio of astaxanthin and nicotinamide riboside is 1:7.

[0082] Example 23

[0083] A composition for eliminating acne and smoothing the skin, which differs from Example 18 in that astaxanthin and nicotinamide riboside are not used in the bioactive ingredients.

[0084] Example 24

[0085] A composition for eliminating acne and smoothing the skin, which differs from Example 18 in that astaxanthin and nicotinamide riboside are not used in the bioactive ingredients.

[0086] Example 25

[0087] A composition for eliminating acne and smoothing the skin, differing from Example 10 in that the bioactive ingredients also include 0.35 parts by weight of efficacy adjuvant B, which is composed of astaxanthin and nicotinamide riboside in a weight ratio of 1:6.

[0088] Comparative Example Comparative Example 1 A composition for eliminating acne and smoothing the skin, which differs from Example 1 in that it removes Lactobacillus paracasei LY12, otherwise it is the same.

[0089] Comparative Example 2 A composition for eliminating acne and smoothing the skin, which differs from Example 1 in that the fermentation extract is removed, otherwise the composition is the same.

[0090] Comparative Example 3 A composition for eliminating acne and smoothing the skin, which differs from Example 1 in that the yeast zinc is removed, otherwise the composition is the same.

[0091] Comparative Example 4 A composition for eliminating acne and smoothing the skin, which differs from Example 1 in that yeast β-glucan is removed, otherwise the composition is the same.

[0092] Comparative Example 5 A composition for eliminating acne and smoothing the skin, differing from Example 1 in that galactooligosaccharides and isomaltooligosaccharides are removed, otherwise the composition is the same.

[0093] Performance testing Test samples: The compositions obtained in Examples 1-25 for eliminating acne and smoothing skin were selected as test samples 1-25, and the compositions obtained in Comparative Examples 1-5 for eliminating acne and smoothing skin were selected as control samples 1-5.

[0094] Experimental Methods: Volunteers aged 18-45 years, regardless of gender, clinically diagnosed with mild to moderate facial acne, who had not used any oral or topical anti-acne medications in the past month, nor any probiotic products or functional skincare products in the past two weeks, and who voluntarily signed informed consent forms.

[0095] Volunteers took one sachet (5g / sachet) of the composition for eliminating acne and smoothing skin orally twice daily, morning and evening, dissolved in warm water, for 28 consecutive days. Control regimens were also administered: Control Regimen 1 consisted of oral minocycline 50mg twice daily, and Control Regimen 2 consisted of topical retinoic acid cream once nightly.

[0096] The acne cure rate (4 weeks) was determined by the percentage of subjects whose facial lesions completely disappeared (excluding active lesions with only pigmentation or superficial scars) at the end of the 4-week treatment period.

[0097] The recurrence rate was determined (3 months after drug discontinuation). Among the subjects who achieved "cure" or "significant effect" at the end of treatment (day 28), the proportion of active acne lesions reappearing on the face (new lesions ≥ 30% of baseline value) 3 months after drug discontinuation follow-up.

[0098] The incidence of adverse reactions was measured, specifically the frequency of adverse events related to the study product that occurred in subjects during treatment and within one week after discontinuation of the drug.

[0099] The test results of test samples 1-25 and control samples 1-5, as well as control scheme 1 and control scheme 2, are recorded in Table 2.

[0100] Table 2 Test Results of Performance Testing

[0101] Combining Examples 1-9 and Control Schemes 1-2 with Table 2, it can be seen that this application achieves its goal of eliminating acne and smoothing skin through the synergistic combination of a prebiotic combination containing galactooligosaccharides and isomaltooligosaccharides, a plant extract containing cherry powder, a fermented extract obtained from purslane-houttuynia cordata-lily through probiotic fermentation, bioactive components containing yeast zinc and yeast β-glucan, and a probiotic powder composed of Lactobacillus paracasei LY12, Lactobacillus plantarum LP28, and Lactobacillus rhamnosus LRH113, as well as a lyophilized compound probiotic powder containing Lactobacillus acidophilus LA1 and Lactobacillus rhamnosus GG. Compared to traditional antibiotics and tretinoin creams, this combination not only has a better therapeutic effect on acne but also reduces the likelihood of recurrence and side effects, ultimately achieving highly effective elimination of acne and smoothing of the skin. Furthermore, considering Comparative Examples 1-5 and Table 2, it can be seen that if any component of the prebiotic combination, plant extracts, fermented extracts, bioactive ingredients, or compound probiotic freeze-dried powder is missing, the acne cure rate (4 weeks), recurrence rate (3 months after discontinuation), and incidence of adverse reactions obtained from the above tests are significantly worse. This demonstrates that the specific combination brings superior results.

[0102] As can be seen from Examples 1 and 10-17 and Table 2, this application, by adding efficacy adjuvant A, which is a compound of inulin and arabinoxylan in a weight ratio of (1.8-2.5):1, to the prebiotic combination, can further improve the acne cure rate (4 weeks) and reduce the recurrence rate (3 months after discontinuation of medication). Furthermore, the combined improvement effect of using inulin or arabinoxylan alone is far less than that of the compound combination. Similarly, as can be seen from Examples 1 and 18-24 and Table 2, by adding efficacy adjuvant B, which is a compound of astaxanthin and nicotinamide riboside in a weight ratio of 1:(5-7), to the bioactive components, can also further improve the acne cure rate (4 weeks) and reduce the recurrence rate (3 months after discontinuation of medication). Furthermore, the combined improvement effect of using astaxanthin or nicotinamide riboside alone is far less than that of the compound combination. Furthermore, in conjunction with Example 25 and Table 2, it can be seen that if the above-mentioned efficacy adjuvant A and efficacy adjuvant B are used together, they can bring about a compound enhancement effect of 1+1>2, ultimately resulting in a composition with better application quality for eliminating acne and smoothing the skin.

[0103] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A composition for eliminating acne and smoothing the skin, characterized in that, It is made from a combination of prebiotics, plant extracts, fermented extracts, bioactive ingredients and lyophilized compound probiotic powder; The prebiotic composition contains raw materials of galactooligosaccharides and isomaltooligosaccharides; The plant extract contains cherry powder as a raw material; The fermented extract was prepared by fermenting purslane, houttuynia cordata, and lily with probiotics. The bioactive components include raw materials for yeast zinc and yeast β-glucan; The compound probiotic freeze-dried powder contains a probiotic powder composed of Lactobacillus paracasei LY12, Lactobacillus plantarum LP28 and Lactobacillus rhamnosus LRH113, Lactobacillus acidophilus LA1 and Lactobacillus rhamnosus GG. The ingredients, by weight, include 0.1-2 parts galactooligosaccharide, 0.1-2 parts isomaltooligosaccharide, 0.1-2 parts cherry powder, 0.1-0.6 parts fermentation extract, 0.1-2 parts yeast zinc, 0.1-2 parts yeast β-glucan, and 0.2-0.5 parts compound probiotic freeze-dried powder. Additionally, each gram of the composition for eliminating acne and smoothing the skin contains: Lactobacillus paracasei LY12 1×10 9 -6×10 9 1×10 probiotic powder composed of CFU, Lactobacillus plantarum LP28 and Lactobacillus rhamnosus LRH113 9 -4×10 9 CFU, Lactobacillus acidophilus LA1 5×10 8 -3×10 9 CFU and Lactobacillus rhamnosus GG 5×10 8 -3×10 9 CFU.

2. The composition for eliminating acne and smoothing skin according to claim 1, characterized in that: In the probiotic powder composed of *Lactobacillus plantarum* LP28 and *Lactobacillus rhamnosus* LRH113, the ratio of live bacteria of *Lactobacillus plantarum* LP28 to *Lactobacillus rhamnosus* LRH113 is 1:

1.

3. The composition for eliminating acne and smoothing skin according to claim 1, characterized in that: The fermentation extract was prepared by the following steps: S1. Take purslane, houttuynia cordata and lily raw materials, crush and mix them, add pure water, heat to 80-100℃ and decoct for 1-2 hours, filter to obtain the first extract; then add pure water to the obtained filter residue, decoct at 80-100℃ for 0.5-1 hours, filter to obtain the second extract; combine the first extract and the second extract and concentrate to obtain the concentrated solution; S2. Inoculate the activated probiotic seed liquid into the concentrate and anaerobic ferment at 37℃ for 40-50h to obtain the fermentation product. After sterilizing the fermentation product, centrifuge to obtain the supernatant, and then concentrate and spray dry under reduced pressure to obtain the fermentation extract.

4. The composition for eliminating acne and smoothing skin according to claim 1, characterized in that: The compound probiotic freeze-dried powder is obtained by mixing probiotic powder composed of Lactobacillus paracasei LY12, Lactobacillus plantarum LP28 and Lactobacillus rhamnosus LRH113, Lactobacillus acidophilus LA1 and Lactobacillus rhamnosus GG in a certain proportion, adding a protectant, resuspending and freeze-drying; the protectant contains skim milk powder, trehalose and monosodium glutamate, and the weight ratio of skim milk powder, trehalose and monosodium glutamate is (2.5-3.5):(1.5-2.5):

1.

5. The composition for eliminating acne and smoothing skin according to claim 1, characterized in that: The prebiotic composition also contains 0.5-1.5 parts by weight of efficacy adjuvant A, which is composed of inulin and arabinoxylan, and the weight ratio of inulin to arabinoxylan is (1.8-2.5):

1.

6. The composition for eliminating acne and smoothing skin according to claim 5, characterized in that: The weight ratio of inulin to arabinoxylan is 2:

1.

7. The composition for eliminating acne and smoothing skin according to claim 1, characterized in that: The bioactive ingredient also contains 0.3-0.4 parts by weight of efficacy adjuvant B, which is composed of astaxanthin and nicotinamide riboside, and the weight ratio of astaxanthin to nicotinamide riboside is 1:(5-7).

8. The composition for eliminating acne and smoothing skin according to claim 7, characterized in that: The weight ratio of astaxanthin to nicotinamide riboside is 1:

6.

9. A method for preparing the composition for eliminating acne and smoothing skin according to claim 1, characterized in that: Includes the following steps: (1) Prepare raw materials containing prebiotic combination, plant extract, fermentation extract, bioactive ingredients and compound probiotic freeze-dried powder according to the formula; (2) After mixing the prebiotic combination, plant extract, fermentation extract and bioactive ingredients in step (1) evenly, add the compound probiotic freeze-dried powder and mix to obtain a composition for eliminating acne and smoothing the skin.

10. The use of the composition for eliminating acne and smoothing skin according to any one of claims 1-8 in the preparation of a pharmaceutical product, health product or cosmetic product for eliminating acne and smoothing skin.

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